CN212152254U - Chemofluorescence probe synthesizer - Google Patents
Chemofluorescence probe synthesizer Download PDFInfo
- Publication number
- CN212152254U CN212152254U CN202020511521.4U CN202020511521U CN212152254U CN 212152254 U CN212152254 U CN 212152254U CN 202020511521 U CN202020511521 U CN 202020511521U CN 212152254 U CN212152254 U CN 212152254U
- Authority
- CN
- China
- Prior art keywords
- synthesis
- sample
- chamber
- incubator body
- incubator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000523 sample Substances 0.000 title claims abstract description 90
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 66
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 66
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 34
- 238000004321 preservation Methods 0.000 claims abstract description 21
- 238000009413 insulation Methods 0.000 claims description 12
- 230000002194 synthesizing effect Effects 0.000 claims description 10
- 239000011521 glass Substances 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 6
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 4
- 239000000725 suspension Substances 0.000 abstract description 3
- 238000012921 fluorescence analysis Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 9
- 238000012258 culturing Methods 0.000 description 5
- 230000001678 irradiating effect Effects 0.000 description 4
- 239000011435 rock Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000001392 ultraviolet--visible--near infrared spectroscopy Methods 0.000 description 1
Images
Abstract
The utility model provides a chemoluminescence probe synthesizer, which comprises a biological incubator, wherein the biological incubator comprises an incubator body and a temperature and humidity controller, a synthesis chamber is arranged on the left side of the incubator body, a sample chamber is arranged on the right side of the incubator body, heat preservation covers are hinged above the synthesis chamber and the sample chamber, a plurality of probe synthesis lamps are uniformly arranged on the surfaces of the two heat preservation covers, and a fluorescence analyzer is arranged at the central position below the two heat preservation covers; a synthesis barrel is arranged in the synthesis chamber, the front side and the rear side of the synthesis barrel are hinged on the inner wall of the incubator body through suspension columns, the rear side of the incubator body is connected with the suspension columns and provided with a hand crank, and a sample culture dish is arranged in the sample chamber; this device increases the sample room in conventional synthesis room one side, makes fluorescent probe can cultivate the sample experiment that corresponds biological cell fluorescent probe in the sample culture dish when doing the synthesis, obtains best synthetic ratio through fluorescence analysis appearance detection, improves fluorescent probe synthetic practicality and pertinence.
Description
Technical Field
The utility model belongs to the technical field of fluorescent probe synthesis equipment, concretely relates to chemical fluorescent probe synthesizer.
Background
A class of fluorescent molecules that fluoresce characteristically in the uv-vis-nir and whose fluorescent properties (excitation and emission wavelengths, intensity, lifetime, polarization, etc.) can change sensitively with changes in the properties of the environment, such as polarity, refractive index, viscosity, etc. Small molecule substances that interact non-covalently with nucleic acids (DNA or RNA), proteins or other macromolecular structures to alter one or more fluorescent properties. Can be used for researching the properties and behaviors of macromolecular substances; the fluorescent probe is most commonly used for labeling antigens or antibodies in a fluorescence immunoassay method, and can also be used for detecting micro characteristics of micro environments, such as surfactant micelles, bimolecular membranes, protein active sites and the like.
In the prior art, when a fluorescent probe is synthesized, a special synthesis device is not provided, and when the activity of different biological cells is analyzed, the fluorescent probe with the optimal proportion needs to be used, the test is needed independently, and because the time, the temperature and the humidity and the irradiation environment of a probe synthesis lamp are inconsistent, the activity of a sample and the activity of an actual synthesized probe to the cells are inconsistent, the test result is influenced, and the performance of the biological cells cannot be really detected.
SUMMERY OF THE UTILITY MODEL
The utility model discloses it does not have professional equipment to solve current fluorescence probe synthesis, need carry out many proportions fluorescence concentration test alone during the synthesis, uses inconvenient problem, provides a chemistry fluorescence probe synthesizer for this.
The utility model provides a technical scheme that its technical problem adopted is:
a chemical fluorescence probe synthesis device comprises a biological incubator, wherein the biological incubator comprises an incubator body and a temperature and humidity controller, a synthesis chamber is installed on the left side of the incubator body, a sample chamber is installed on the right side of the incubator body, heat preservation covers are hinged above the synthesis chamber and the sample chamber, a plurality of probe synthesis lamps are uniformly installed on the lower surfaces of the two heat preservation covers, and a fluorescence analyzer is installed at the central position below the two heat preservation covers; install synthetic bucket in the synthetic chamber, both sides articulate on incubator body inner wall through hanging the post around the synthetic bucket, incubator body rear side is connected and is hung the post and install the crank, install the sample culture dish in the sample chamber.
Furthermore, two glass covers are arranged below the heat preservation covers and cover the outer sides of the probe synthesis lamps.
Further, a heat insulation plate is arranged in the synthesis chamber and above the outer side of the synthesis barrel, and the height of the barrel opening of the synthesis barrel is lower than that of the heat insulation plate; and the sample chamber is internally provided with a heat insulation plate outside the sample culture dish.
Preferably, the bottom of the sample culture dish is a stepped culture surface.
Preferably, the bottom of the sample culture dish is an inclined culture surface.
Compared with the prior art, the beneficial effects of the utility model are as follows:
this device increases the sample room in conventional synthesis room one side, makes fluorescent probe can cultivate the sample experiment that corresponds biological cell fluorescent probe in the sample culture dish when doing the synthesis, obtains best synthetic ratio through fluorescence analysis appearance detection, improves fluorescent probe synthetic practicality and pertinence. When synthesizing the fluorescent probe, culturing the corresponding biological cells to be tested in the sample culture dish, uniformly dripping a fluorescent probe sample on the bottom of the sample culture dish from a high position to a bottom surface, enabling the bottom of the sample culture dish to present fluorescent concentrations with different levels and concentrations from high to low, completing activity test by irradiating rays of the same probe synthesis lamp, detecting the obtained fluorescent probe concentration through a fluorescence analyzer, uploading the activity degree of the biological cells to a computer end to obtain a corresponding data table, thus obtaining the optimal proportion, performing proportion synthesis in a synthesis barrel, irradiating rays by using the same probe synthesis lamp, detecting through the fluorescence analyzer, and synthesizing the fluorescent probe with pertinence and the most suitable for the corresponding biological cells.
Drawings
The present invention will be further explained with reference to the drawings and examples.
Fig. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a sample petri dish according to a first embodiment of the invention;
FIG. 3 is a cross-sectional view of a sample petri dish in a second embodiment of the invention;
the embodiment of the utility model provides a contain following most main reference character:
the device comprises a biological incubator-1, an incubator body-101, a synthesis chamber-2, a synthesis barrel-201, a suspension column-202, a hand rocker-203, a sample chamber-3, a sample culture dish-301, a heat preservation cover-4, a probe synthesis lamp-5, a fluorescence analyzer-6, a glass cover-7 and a heat preservation plate-8.
Detailed Description
Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention. On the contrary, the embodiments of the invention include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", and the like, indicate orientations and positional relationships based on the orientations and positional relationships shown in the drawings, and are used only for convenience in describing and simplifying the present invention, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example one
As shown in fig. 1, a chemical fluorescence probe synthesizer comprises a biological incubator 1, wherein the biological incubator 1 comprises an incubator body 101 and a temperature and humidity controller, a synthesis chamber 2 is installed on the left side of the incubator body 101, a sample chamber 3 is installed on the right side of the incubator body 101, heat preservation covers 4 are hinged above the synthesis chamber 2 and the sample chamber 3, a plurality of probe synthesis lamps 5 are uniformly installed on the lower surfaces of the two heat preservation covers 4, and a fluorescence analyzer 6 is installed at the central position below the two heat preservation covers 4; install synthetic bucket 201 in the synthetic chamber 2, both sides articulate on incubator body 101 inner wall through hanging post 202 around the synthetic bucket 201, incubator body 101 rear side is connected and is hung post 202 and install crank 203, slightly rocks synthetic bucket 201 through crank 203 and mixes and react, avoids solving current electrical equipment and rocks the problem that synthetic bucket 201 heat production makes the cell inefficacy, install sample culture dish 301 in the sample room 3.
The sample chamber 3 is additionally arranged on one side of the conventional synthesis chamber 2, so that the fluorescent probe can be used for culturing a sample experiment corresponding to the biological cell fluorescent probe in the sample culture dish 301 during synthesis, the optimal synthesis ratio is obtained through detection of the fluorescent analyzer 6, and the practicability and pertinence of the fluorescent probe synthesis are improved.
Two glass covers 7 are installed below the heat preservation cover 4, and the two glass covers 7 cover the outer side of the probe synthesis lamp 5.
An insulation board 8 is arranged above the outer side of the synthesis barrel 201 in the synthesis chamber 2, and the height of the barrel opening of the synthesis barrel 201 is lower than that of the insulation board 8; and a heat insulation plate 8 is arranged on the outer side of the sample culture dish 301 in the sample chamber 3.
As shown in FIG. 2, the bottom of the sample culture dish 301 is a stepped culture surface.
When synthesizing the fluorescent probe, culturing the biological cells to be tested correspondingly in the sample culture dish 301, uniformly dripping a fluorescent probe sample on the bottom of the sample culture dish 301 from a high position to the bottom surface, so that the stepped bottom surface of the sample culture dish 301 has fluorescent concentrations with different levels and concentrations, completing activity test by irradiating the same probe synthesis lamp 5 with rays, obtaining the fluorescent probe concentration through detection of a fluorescence analyzer 6, uploading the activity degree of the biological cells to a computer end to obtain a corresponding data table, thus obtaining the optimal proportion, performing proportion synthesis in the synthesis barrel 201, irradiating the same probe synthesis lamp 5 with rays, detecting through the fluorescence analyzer 6, and synthesizing the fluorescent probe with pertinence and the most suitable for the corresponding biological cells.
Example two
As shown in fig. 1, a chemical fluorescence probe synthesizer comprises a biological incubator 1, wherein the biological incubator 1 comprises an incubator body 101 and a temperature and humidity controller, a synthesis chamber 2 is installed on the left side of the incubator body 101, a sample chamber 3 is installed on the right side of the incubator body 101, heat preservation covers 4 are hinged above the synthesis chamber 2 and the sample chamber 3, a plurality of probe synthesis lamps 5 are uniformly installed on the lower surfaces of the two heat preservation covers 4, and a fluorescence analyzer 6 is installed at the central position below the two heat preservation covers 4; install synthetic bucket 201 in the synthetic chamber 2, both sides articulate on incubator body 101 inner wall through hanging post 202 around the synthetic bucket 201, incubator body 101 rear side is connected and is hung post 202 and install crank 203, slightly rocks synthetic bucket 201 through crank 203 and mixes and react, avoids solving current electrical equipment and rocks the problem that synthetic bucket 201 heat production makes the cell inefficacy, install sample culture dish 301 in the sample room 3.
The sample chamber 3 is additionally arranged on one side of the conventional synthesis chamber 2, so that the fluorescent probe can be used for culturing a sample experiment corresponding to the biological cell fluorescent probe in the sample culture dish 301 during synthesis, the optimal synthesis ratio is obtained through detection of the fluorescent analyzer 6, and the practicability and pertinence of the fluorescent probe synthesis are improved.
Two glass covers 7 are installed below the heat preservation cover 4, and the two glass covers 7 cover the outer side of the probe synthesis lamp 5.
An insulation board 8 is arranged above the outer side of the synthesis barrel 201 in the synthesis chamber 2, and the height of the barrel opening of the synthesis barrel 201 is lower than that of the insulation board 8; and a heat insulation plate 8 is arranged on the outer side of the sample culture dish 301 in the sample chamber 3.
As shown in FIG. 2, the bottom of the sample petri dish 301 is an inclined culture surface.
When synthesizing the fluorescent probe, culturing the biological cells to be tested correspondingly in the sample culture dish 301, uniformly dripping the fluorescent probe sample from a high position to a bottom surface at the bottom of the sample culture dish 301, leading the inclined surface of the sample culture dish 301 from high to low to present fluorescent concentrations with different levels and concentrations, completing activity test by the radiation irradiation of the same probe synthesis lamp 5, obtaining the fluorescent probe concentration by the detection of a fluorescent analyzer 6, uploading the fluorescent probe concentration to a computer end to obtain a corresponding data table according to the activity degree of the biological cells, thereby obtaining the optimal proportion, then performing proportion synthesis in the synthesis barrel 201, using the same probe synthesis lamp 5 for radiation irradiation, and detecting by the fluorescent analyzer 6, and synthesizing the fluorescent probe with pertinence and the most suitable for the corresponding biological cells.
In light of the foregoing, it will be apparent to those skilled in the art from this disclosure that various changes and modifications can be made without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (5)
1. The utility model provides a chemical fluorescence probe synthesizer, includes biological incubator (1), biological incubator (1) is including incubator body (101) and atmospheric control ware, its characterized in that: a synthesis chamber (2) is installed on the left side of the incubator body (101), a sample chamber (3) is installed on the right side of the incubator body (101), heat preservation covers (4) are hinged to the upper portions of the synthesis chamber (2) and the sample chamber (3), a plurality of probe synthesis lamps (5) are uniformly installed on the lower surfaces of the two heat preservation covers (4), and a fluorescence analyzer (6) is installed in the center position below the two heat preservation covers (4); install synthetic bucket (201) in synthetic chamber (2), both sides articulate on incubator body (101) inner wall through hanging post (202) around synthetic bucket (201), incubator body (101) rear side is connected and is hung post (202) and install crank (203), install sample culture dish (301) in sample room (3).
2. The apparatus for synthesizing a fluorescent probe according to claim 1, wherein: two glass covers (7) are installed below the heat preservation cover (4), and the two glass covers (7) cover the outer side of the probe synthetic lamp (5).
3. The apparatus for synthesizing a fluorescent probe according to claim 2, wherein: an insulation board (8) is arranged above the outer side of the synthesis barrel (201) in the synthesis chamber (2), and the height of the barrel opening of the synthesis barrel (201) is lower than that of the insulation board (8); and a heat insulation plate (8) is arranged on the outer side of the sample culture dish (301) in the sample chamber (3).
4. The apparatus for synthesizing fluorescent probe according to claim 3, wherein: the bottom of the sample culture dish (301) is a step-shaped culture surface.
5. The apparatus for synthesizing fluorescent probe according to claim 3, wherein: the bottom of the sample culture dish (301) is an inclined culture surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020511521.4U CN212152254U (en) | 2020-04-09 | 2020-04-09 | Chemofluorescence probe synthesizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020511521.4U CN212152254U (en) | 2020-04-09 | 2020-04-09 | Chemofluorescence probe synthesizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212152254U true CN212152254U (en) | 2020-12-15 |
Family
ID=73725587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020511521.4U Expired - Fee Related CN212152254U (en) | 2020-04-09 | 2020-04-09 | Chemofluorescence probe synthesizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212152254U (en) |
-
2020
- 2020-04-09 CN CN202020511521.4U patent/CN212152254U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ou et al. | Microfluidic chip electrophoresis for biochemical analysis | |
| Ruzicka et al. | Peer Reviewed: From Flow Injection to Bead Injection. | |
| Wehry | Modern fluorescence spectroscopy | |
| Sonnleitner | Automated measurement and monitoring of bioprocesses: key elements of the M 3 C strategy | |
| US7041493B2 (en) | Bioreactor and bioprocessing technique | |
| Lu et al. | Recent developments in single-cell analysis | |
| CN108593916A (en) | Cancer detection system and method based on excretion body | |
| CN1251609A (en) | Method and product for analyzing polymers | |
| JPH10160705A (en) | Capillary electrophoretic device | |
| CN106833623A (en) | A kind of fluorescence probe and preparation method thereof | |
| CN102183504B (en) | Microfluidic unicellular active oxygen automatic analyzer | |
| Croney et al. | Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations | |
| US20120237950A1 (en) | Analysis chip, analysis system, and analysis method | |
| CN105928920A (en) | Detection method based on aggregation-induced emission and aptamers | |
| KR20140111192A (en) | Parallel line biochip for multiplex diagnosis | |
| Shoemaker et al. | Multiple sampling in single-cell enzyme assays using CE-laser-induced fluorescence to monitor reaction progress | |
| CN212152254U (en) | Chemofluorescence probe synthesizer | |
| KR20150112913A (en) | Parallel line biochip for multiplex diagnosis | |
| CN208156013U (en) | Cancer detection system based on excretion body | |
| CN202562928U (en) | Visualized protein chip device | |
| CN103454268B (en) | A kind of reducing sugar quantitative detecting method based on click-reaction | |
| CN202201884U (en) | Kit for cell biotechnology | |
| CN115015176B (en) | Optical diffraction tomography enhancement method and device | |
| CN108774325A (en) | The synthetic method of metal coordinating polymer and its application in the detection of diamines substance and equipment | |
| CN200941103Y (en) | Combined high flux album micro-array chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201215 |