CN208443738U - A kind of new structural SERS substrate - Google Patents
A kind of new structural SERS substrate Download PDFInfo
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- CN208443738U CN208443738U CN201821279091.7U CN201821279091U CN208443738U CN 208443738 U CN208443738 U CN 208443738U CN 201821279091 U CN201821279091 U CN 201821279091U CN 208443738 U CN208443738 U CN 208443738U
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- grain
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- nano wire
- atp
- atp aptamer
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Abstract
The utility model discloses a kind of new structural SERS substrates, including Fe3O4@Ag-ATP aptamer, AAO template.Wherein Fe3O4@Ag-ATP aptamer structure includes Fe3O4Nano wire, silver nano-grain, ATP aptamer, wherein silver nano-grain is combined closely in Fe3O4On nano wire, the silver nano-grain is in Fe3O4Free arrangement on nano wire has gap between particle, and ATP aptamer connects in the gap between silver nano-grain and and Fe3O4Nano wire is combined closely, Fe3O4Nano wire is grown in AAO template.The structure combination SERS technology establishes simple, quick, sensitive and accurate ATP identification and detection method, has expanded application of the SERS technology in terms of life science, facilitates the fields such as clinical diagnosis, Pharmaceutical Analysis and drug development and shortens detection time.
Description
Technical field
The utility model belongs to spectrum analysis detection technique field, is related to enhancing Raman signal field, and in particular to a kind of
Raman spectrum base.
Background technique
Atriphos (ATP), as " the energy currency " of intracellular energy transmitting, storage and transmitting chemical energy, extensively
It is present in biological cell, provides energy for the vital movement of organism, is the necessary factor of cells survival.It establishes easy to be fast
Victory, highly sensitive and highly selective ATP identification and detection technique not only facilitate the research for pushing life process, comment in drug
The fields such as valence, drug development, clinical diagnosis, microbial contamination detection also have important practical significance.Paper " the detection side ATP
Method progress " in tell about the detection method of traditional ATP include chromatography, electrophoresis and Optical Analysis Method；For chromatography, skill
Art is mature, simple and quick, but its sensitivity is only mM grade；Electrophoresis has high sensitivity, but its is complicated for operation；Optics point
Analysis method non-destructive testing, fast and easy, but the sample ATP extracting method used in every kind of method mention ATP in complex sample
It takes but optimistic not to the utmost.
SERS technology is a kind of emerging analytical technology, has simple pre-treatment, easy to operate, sensitivity and selectivity
It is high, the unique technical advantage such as limit by sample topography, can be carried out non-destructive testing, have in terms of chemistry, biological detection and be cured
It is widely applied, this technology can be used in the quantitative detection of ATP to be cured.And having the active substrate of excellent SERS is that generation is excellent
The prerequisite of elegant SERS signal, the preparation of substrate have a very important influence for SERS, existing SERS substrate there is
Reproducibility is undesirable, the holding time is short, the bad regulation of substrate, the disadvantages of specificity is not strong, causes SERS technology to be applied to quantitative
There are bigger difficulty for analysis.If number of patent application is 201310352817.0, patent name is a kind of SERS nano silver-group
The patent of invention of the preparation method at bottom, have the advantages that simple process, it is easy to operate, prepare it is quick, but prepare substrate it is special
Property is not strong, is difficult to the quantitative detection of microorganism.
Summary of the invention
For the above-mentioned problems in the prior art, the purpose of this utility model is that by the specific recognition of ATP and
SERS technology, which is combined together, provides a kind of novel SERS substrate, its purpose is to provide a kind of stability is strong, hotspot's distribution
Uniformly, high sensitivity, the SERS substrate of high specificity.
In order to solve the above problem, the utility model adopts the following technical solution:
A kind of new structural SERS substrate, including Fe3O4@Ag-ATP aptamer (1), AAO template (2), Fe3O4Nano wire
(3), silver nano-grain (4), ATP aptamer (5)；Fe3O4@Ag-ATP aptamer (1) structure includes Fe3O4Nano wire (3), silver nanoparticle
Particle (4), ATP aptamer (5), the Fe3O4Nano wire (3) is constructed by AAO template (2), and AAO template (2) is for Fe3O4It receives
The region of rice noodles (3) growth is arranged and is separated from each other, Fe by multirow or multiple row3O4Nano wire (3) is vertically arranged in AAO template (2)
Growth district on；Silver nano-grain (4), ATP aptamer (5) are combined closely in Fe3O4On nano wire (3).The silver nanoparticle
Grain (4) is integrated to Fe by magnetron sputtering method3O4On nano wire (3), silver nano-grain (4) is in Fe3O4The position of nano wire (3)
Free arrangement has gap between particle.ATP aptamer (5) connects in the gap between silver nano-grain (4) and and Fe3O4Nano wire
(3) it combines closely.
Above-mentioned technical proposal has the following beneficial effects:
1. the detection of SERS technology and ATP is combined, a kind of novel SERS substrate is provided, can be used for quantifying for ATP
Detection, the choice of the substrates is strong, high sensitivity, and hotspot's distribution is uniform.
2. electrochemical method is combined with mould plate technique, limited using the space of filling and hole to AAO
Nano-material is prepared, manufactured SERS substrate is evenly distributed, spacing-controllable, can generate more outstanding SERS signal, improves
The accuracy of detection provides new approach for the preparation of high request SERS substrate.
3. strong interaction and SERS specific between ATP aptamer and ATP are combined, SERS has been widened in biochemistry
The application range of analysis field also improves the detection sensitivity to object.
Detailed description of the invention
With reference to the accompanying drawing and embodiment the utility model is described in further detail.
Fig. 1 is SERS underlying structure figure；
Fig. 2 is SERS substrate top view；
Fig. 3 is Fe3O4@Ag-ATP aptamer structure partial enlarged view；
1 is Fe3O4@Ag-ATP aptamer, 2 be AAO template, 3 be Fe3O4Nano wire, 4 be silver nano-grain, 5 be ATP suitable
Body.
Specific embodiment
It is carried out below with reference to technical effect of the embodiment and attached drawing to the design of the utility model, specific structure and generation
It clearly and completely describes, to be completely understood by the purpose of this utility model, feature and effect.Obviously, described embodiment
It is a part of the embodiment of the utility model, rather than whole embodiments, it is based on the embodiments of the present invention, the skill of this field
Art personnel other embodiments obtained without creative efforts belong to the model of the utility model protection
It encloses.
In Fig. 1, the utility model SERS substrate, including Fe3O4@Ag-ATP aptamer 1, AAO template 2, Fe3O4Nano wire 3,
Silver nano-grain 4, ATP aptamer 5；Wherein Fe3O41 structure of@Ag-ATP aptamer includes Fe3O4Nano wire 3, silver nano-grain 4, ATP
Aptamer 5, Fe3O4Nano wire 3 is constructed by AAO template 2；Silver nano-grain 4, ATP aptamer 5 are combined closely in Fe3O4Nano wire 3
On.There is gap between silver nano-grain 4, ATP aptamer 5 is closely adhered to Fe by the gap between silver nano-grain 43O4Nanometer
On line 3.
In Fig. 2, the utility model includes Fe3O4@Ag-ATP aptamer 1, AAO template 2；Fe3O4@Ag-ATP aptamer 1 is uniformly tied
It closes in AAO template 2.
In Fig. 3, the utility model Fe3O4@Ag-ATP aptamer structure includes Fe3O4Nano wire 3, silver nano-grain 4, ATP are suitable
Body 5；Silver nano-grain 4 is integrated into Fe3O4On nano wire 3, and between have a gap, ATP aptamer 5 passes through between silver nano-grain 4
Gap is closely adhered to Fe3O4On nano wire 3.
Further, the silver nano-grain 4 is in Fe3O4On nano wire 3, silver nano-grain 4 is in Fe3O4Nano wire 3
Position free arrangement has gap between particle.
Further, the Fe3O4Nano wire 3 is constructed by AAO template 2, and AAO template 2 is for Fe3O4Nano wire 3 is grown
Region arranged and be separated from each other, Fe by multirow or multiple row3O4Nano wire 3 is vertically arranged on the growth district of AAO template 2；Silver
Nano particle 4, ATP aptamer 5 are combined closely in Fe3O4On nano wire 3.
The better embodiment of the utility model is illustrated above, but the utility model creation is not limited to
The embodiment, those skilled in the art can also make various etc. without departing from the spirit of the present invention
Same variation or replacement, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (3)
1. a kind of new structural SERS substrate, including Fe3O4@Ag-ATP aptamer (1), AAO template (2), Fe3O4Nano wire (3),
Silver nano-grain (4), ATP aptamer (5)；Fe3O4@Ag-ATP aptamer (1) structure includes Fe3O4Nano wire (3), silver nano-grain
(4), ATP aptamer (5), which is characterized in that Fe3O4Nano wire (3) is constructed by AAO template (2), and AAO template (2) is for Fe3O4It receives
The region of rice noodles (3) growth is arranged and is separated from each other, Fe by multirow or multiple row3O4Nano wire (3) is vertically arranged in AAO template (2)
Growth district on；Silver nano-grain (4), ATP aptamer (5) are combined closely in Fe3O4On nano wire (3).
2. a kind of new structural SERS substrate according to claim 1, it is characterised in that silver nano-grain (4) passes through magnetic
Control sputtering method is integrated to Fe3O4On nano wire (3), silver nano-grain (4) is in Fe3O4The position free arrangement of nano wire (3),
Intergranular has gap.
3. a kind of new structural SERS substrate according to claim 1, it is characterised in that silver nano-grain has between (4)
Gap, ATP aptamer (5) connects in the gap between silver nano-grain (4) and and Fe3O4Nano wire (3) is combined closely.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821279091.7U CN208443738U (en) | 2018-08-09 | 2018-08-09 | A kind of new structural SERS substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821279091.7U CN208443738U (en) | 2018-08-09 | 2018-08-09 | A kind of new structural SERS substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208443738U true CN208443738U (en) | 2019-01-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821279091.7U Expired - Fee Related CN208443738U (en) | 2018-08-09 | 2018-08-09 | A kind of new structural SERS substrate |
Country Status (1)
| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113030064A (en) * | 2021-05-27 | 2021-06-25 | 北京市疾病预防控制中心 | Surface-enhanced Raman scattering substrate and preparation method and application thereof |
-
2018
- 2018-08-09 CN CN201821279091.7U patent/CN208443738U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113030064A (en) * | 2021-05-27 | 2021-06-25 | 北京市疾病预防控制中心 | Surface-enhanced Raman scattering substrate and preparation method and application thereof |
| CN113030064B (en) * | 2021-05-27 | 2021-08-10 | 北京市疾病预防控制中心 | Surface-enhanced Raman scattering substrate and preparation method and application thereof |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190129 Termination date: 20190809 |
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| CF01 | Termination of patent right due to non-payment of annual fee |