CN106896050A - A kind of blood counting chamber - Google Patents

A kind of blood counting chamber Download PDF

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Publication number
CN106896050A
CN106896050A CN201710228099.4A CN201710228099A CN106896050A CN 106896050 A CN106896050 A CN 106896050A CN 201710228099 A CN201710228099 A CN 201710228099A CN 106896050 A CN106896050 A CN 106896050A
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China
Prior art keywords
counting chamber
liquid
sample liquid
base plate
piece
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CN201710228099.4A
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Chinese (zh)
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CN106896050B (en
Inventor
吕纯莉
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Affiliated Hospital of Zunyi Medical University
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Affiliated Hospital of Zunyi Medical University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a kind of blood counting chamber in biological equipment field;Including base plate and cover plate, the upper surface of base plate is provided with counting chamber;Be additionally provided with base plate can thereon surface movement every liquid piece.This programme is by setting every liquid piece, when in use, sample liquid unnecessary in counting chamber can be released outside counting chamber every liquid piece, so that the actual volume amount of sample liquid is consistent with the volume of counting chamber in counting chamber, the actual volume for avoiding sample liquid in counting chamber occurs more than or less than the situation of counting chamber volume, so as to improve the precision of sample liquid counting.

Description

A kind of blood counting chamber
Technical field
The present invention relates to biological equipment field, more particularly to a kind of blood counting chamber.
Background technology
Blood counting chamber is used to carry out microscopic count to red in human body, white blood cell to be used, and is also commonly used for calculating some thin The quantity of the microorganisms such as bacterium, fungi, yeast, is a kind of common biology instrument.Especially in disease diagnosis and therapy process In, generally require to use blood counting chamber to detect the cell in patient in blood, can be with by the counting to cell and classification For clinical disease make a definite diagnosis or the observation for the treatment of effect provides important evidence.
Current commonly used blood counting chamber has " ox Bao tally " and " the quantitative sediment analysis plates of Fast-read10 " etc., By taking " the quantitative sediment analysis plates of Fast-read10 " as an example, 10 block plaids are provided with this tally altogether, each block plaid Volume is 0.1 μ l, and every block of plate can apply to 10 cell counts of sample.But when cell number is more, work can be caused Counting of the personnel to block plaid inner cell is difficult, so as to cause result error larger.In view of the situation, at present to " Fast- The improvement of the quantitative sediment analysis plates of read10 ", is mainly evenly divided into 9 medium squares by each of which block plaid, then by each Most middle medium square is subdivided into 9 lattices in block plaid, and the cell in 9 lattices is counted under high power lens, And then the cell number contained in every liter of sample liquid can be extrapolated.
But during the grid injection sample liquid on to tally, artificial drop is taken because injection mode is mainly Note, the sample liquid of instillation often occurs the situation overflowed grid or do not fill grid, and is according to each block plaid when counting Volume, so as to cause deviation occur to the counting of sample liquid inner cell, causes the false diagnosis of disease come what is calculated, can be endangered when serious Health of the evil to patient.
The content of the invention
For above-mentioned technical problem, the invention is intended to provide a kind of blood counting chamber, to solve the tally of prior art Sample liquid injection rate does not know in grid, causes cell count the problem of deviation occur.
A kind of blood counting chamber in this programme, including base plate and cover plate, the upper surface of base plate is provided with counting chamber;On base plate Be additionally provided with can thereon surface movement every liquid piece.
The operation principle and its advantage of this programme:Base plate be provided with can thereon surface movement every liquid piece;Use When, after sample measuring liquid to be checked is injected in the counting chamber on base plate, allow sample liquid to overflow counting chamber a little;Then make every liquid piece in base plate Upper movement, during being moved every liquid piece, promotes unnecessary sample liquid in counting chamber to move forward every liquid piece, until by unnecessary sample Liquid is released outside counting chamber, so as to ensure that the sample liquid given volume in counting chamber, it is to avoid the sample liquid actual volume in counting chamber Amount is more than or less than the situation of counting chamber volume, so as to ensure that the accuracy of sample liquid cell count.During counting, cover plate is put Put is carried out above counting chamber, and cell count is carried out using microscope, due to the stop of cover plate, it is to avoid microscope and sample liquid Directly contact, so as to ensure that the accuracy of microscopical health and detection.
Further, the counting chamber is provided with multiple.By setting multiple counting chambers, can be with same blood counting chamber Multiple different types of sample liquids are detected, so as to improve the detection efficiency of sample liquid.
Further, it is provided with crisscross intake chute between the adjacent counting chamber.By setting intake chute so that every liquid piece The unnecessary sample liquid released can be discharged by intake chute, it is to avoid sample liquid is piled up on base plate, is polluted.
Further, the cover plate is provided with button along the catching groove for coordinating with button edge around counting chamber.Cover plate is set to carry Button edge, and coordinate with catching groove, when cell count is carried out, due to being fitted close between button edge and catching groove so that cover plate is close to meter The top in number ponds, it is to avoid rocking for cover plate, so as to improve the accuracy of counting.
Further, the catching groove is connected with intake chute.Catching groove is connected with intake chute, many in the pushing course every liquid piece Remaining sample liquid to being flowed into catching groove, then flows out hemocytometer by after liquid piece releases counting chamber by the intake chute connected with catching groove Number plate, so as to reduce flowing of the unnecessary sample liquid on base plate upper surface, is conducive to keeping the cleaning of tally.
Further, it is described to be provided with through hole every liquid piece.By setting through hole, can be by through hole to injecting sample in counting chamber Liquid, because the diameter comparative counting pond of through hole is much smaller, and is brought into close contact every liquid piece and base plate, is injected when in counting chamber Sample liquid fill counting chamber after, can quickly be found by through hole, it is to avoid the waste of sample liquid;And due to unnecessary in counting chamber Sample liquid content is reduced, and is also beneficial to keep the cleaning of tally.
Further, the side wall every liquid piece is provided with the fin for upwardly extending.Promoting unnecessary in counting chamber every liquid piece Sample liquid outflow after, after unnecessary sample liquid is excessive, sample liquid can exceed every the upper surface of liquid piece being piled up every the front end of liquid piece, lead to Cross setting fin so that sample liquid is difficult to be higher by every liquid piece, all the time every the flowing of the front of liquid piece;So as to avoid every the upper of liquid piece End face is contaminated.
Further, the side wall every liquid piece is provided with absorbent sheet, and the bottom surface of absorbent sheet is higher than every the bottom surface of liquid piece.Pass through Absorbent sheet is set, when promoting unnecessary sample liquid to flow every liquid piece, when the sample liquid in front of liquid piece is stacked into a certain amount of, absorbent sheet Unnecessary sample liquid is absorbed, reduces sample liquid content, so as to reduce pollution of the sample liquid to base plate.And the bottom surface of absorbent sheet is high In every the bottom surface of liquid piece so that the bottom surface of absorbent sheet is consistently higher than counting chamber, sample in counting chamber is absorbed so as to avoid absorbent sheet The situation of liquid occurs, it is ensured that the sample liquid given volume in counting chamber, so as to be conducive to improving the counting precision of sample liquid.
Brief description of the drawings
Fig. 1 is a kind of structural representation of blood counting chamber of the invention;
Fig. 2 is a kind of another structural representation of blood counting chamber of the invention.
Specific embodiment
Below by specific embodiment, the present invention is further detailed explanation:
Reference in Figure of description includes:Base plate 1, counting chamber 2, counting lattice 3, intake chute 4, catching groove 5, support column 6, company Extension bar 7, every liquid piece 8, through hole 9, fin 10, absorbent sheet 11, pad 12.
Embodiment 1:
A kind of blood counting chamber as shown in Figure 1, including base plate 1 and the cover plate with button edge, the upper surface of base plate 1 is provided with four Counting chamber 2, is provided with button along the catching groove 5 for coordinating on base plate 1 around counting chamber 2;Each counting chamber 2 is divided into eight counting lattice 3 Volume be 0.1mm3;Two crisscross intake chutes 4 are provided between adjacent counting chamber 2, wherein an intake chute 4 runs through base plate 1 two ends;Connected between intake chute 4 and catching groove 5, the depth of intake chute 4 is more than catching groove 5;Support column is slidably connected in intake chute 4 6, support column 6 by
First support column of " L " shape and the second support column are constituted;The both sides of support column 6 are connected with telescopic connecting rod 7, connection The other end of bar 7 is connected with every liquid piece 8, is fitted with the upper surface of base plate 1 every the lower surface of liquid piece 8;6 are provided with every liquid piece 8 Through hole 9;The fin 10 for upwardly extending is equipped near the side of counting chamber 2 and its opposite side every liquid piece 8;Fin 10 is provided with suction Liquid piece 11, the bottom surface of absorbent sheet 11 is higher than every the bottom surface of liquid piece 8.
When using, mobile support column 6 makes the through hole 9 on support column 6 be located at the top for counting lattice 3, by through hole 9 to meter Injection sample liquid in number lattice 3, after sample liquid fills up counting chamber 2, sample liquid can assemble in through hole 9, now stop the injection of sample liquid, push away Dynamic support column 6, the movement of support column 6 can forward promote sample liquid unnecessary in counting chamber 2, unnecessary due to being provided with fin 10 Sample liquid cannot be introduced into every the upper surface of liquid piece 8, while absorbent sheet 11 can also absorb to the sample liquid being in contact with it, so as to subtract Unnecessary sample liquid, keeps the cleaning of tally on few base plate 1.Meanwhile, the sample liquid not absorbed by absorbent sheet 11 is by catching groove 5 and leads Outside the outflow tally of liquid bath 4;After liquid piece 8 is promoted on base plate 1, because the sample liquid for counting unnecessary in lattice 3 is pushed away every liquid piece 8 Walk, be 0.1mm3 so as to ensure that each counts the sample liquid volume in lattice 3;Then cover plate is fastened in catching groove 5, using micro- Mirror is counted to the cell in sample liquid, so as to ensure that technology acuracy.Simultaneously because connecting rod 7 is scalable, can be by stretching Strut of drawing money on credit will stretch out every liquid piece 8 and be cleaned outside base plate 1;It is easy to use next time, it is to avoid pollution.
Embodiment 2:
A kind of blood counting chamber as shown in Figure 2, it is with the difference of embodiment 1, and two intake chutes 4 run through base plate 1 two ends, support column 6 is cylinder, and one end of support column 6 is connected to the intersection of two intake chutes 4;Connecting rod 7 rotates connection On support column 6, pad 12 is provided between connecting rod 7 and base plate 1;It is strip every liquid piece 8, it is more than meter every the length of liquid piece 8 The most long-diagonal in number pond 2.
Tally in embodiment 1 and embodiment 2 can according to the actual requirements increase or decrease counting chamber 2 when making And count the setting of lattice 3;The volume for counting lattice 3 can also be changed.
The difference of blood counting chamber in embodiment 2 when in use with embodiment 1 is, in embodiment 2 every liquid , it is necessary to rotate every liquid piece 8 when piece is removed to unnecessary sample liquid.
Above-described is only embodiments of the invention, and the general knowledge such as known concrete structure and characteristic is not made herein in scheme Excessive description.It should be pointed out that for a person skilled in the art, on the premise of structure of the present invention is not departed from, can be with Some deformations and improvement are made, these should also be considered as protection scope of the present invention, these are implemented all without the influence present invention Effect and practical applicability.This application claims protection domain should be defined by the content of its claim, in specification Specific embodiment etc. records the content that can be used for explaining claim.

Claims (8)

1. a kind of blood counting chamber, including base plate and cover plate, the upper surface of base plate are provided with counting chamber;Characterized in that, on base plate Be additionally provided with can thereon surface movement every liquid piece.
2. blood counting chamber according to claim 1, it is characterised in that:The counting chamber is provided with multiple.
3. blood counting chamber according to claim 2, it is characterised in that:It is provided with crisscross between the adjacent counting chamber Intake chute.
4. the blood counting chamber according to claim 1 or 3, it is characterised in that:The cover plate is with button edge, the week of counting chamber Enclose and be provided with button along the catching groove for coordinating.
5. blood counting chamber according to claim 4, it is characterised in that:The catching groove is connected with intake chute.
6. blood counting chamber according to claim 1 or 5, it is characterised in that:It is described to be provided with through hole every liquid piece.
7. blood counting chamber according to claim 6, it is characterised in that:The side wall every liquid piece is provided with and upwardly extends Fin.
8. the blood counting chamber according to claim 1 or 7, it is characterised in that:The side wall every liquid piece is provided with imbibition Piece, the bottom surface of absorbent sheet is higher than every the bottom surface of liquid piece.
CN201710228099.4A 2017-04-10 2017-04-10 Blood cell counting plate Active CN106896050B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710228099.4A CN106896050B (en) 2017-04-10 2017-04-10 Blood cell counting plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710228099.4A CN106896050B (en) 2017-04-10 2017-04-10 Blood cell counting plate

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CN106896050A true CN106896050A (en) 2017-06-27
CN106896050B CN106896050B (en) 2023-08-29

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Citations (22)

* Cited by examiner, † Cited by third party
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SU57672A1 (en) * 1938-11-09 1939-11-30 И.Н. Декаристо A device for counting and arranging seeds, etc.
GB1193994A (en) * 1967-07-27 1970-06-03 Vincent John Tune Improvements in or relating to a Counting Device
US4065000A (en) * 1976-06-03 1977-12-27 Gary Murton Pharmaceutical tablet and capsule counter
US4726237A (en) * 1985-06-19 1988-02-23 Sequoia-Turner Corporation Fluid metering apparatus and method
CN2175433Y (en) * 1993-11-09 1994-08-24 金华钧 Counting means for microscope
US5876604A (en) * 1996-10-24 1999-03-02 Compact Membrane Systems, Inc Method of gasifying or degasifying a liquid
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CN200956009Y (en) * 2006-06-12 2007-10-03 河南农业大学 Device for counting eggs by microscope
CN101160520A (en) * 2005-04-08 2008-04-09 布勒医药股份公司 Apparatus for filling a sample volume defining device
CN201229288Y (en) * 2008-07-28 2009-04-29 南京千盛医疗设备制造有限公司 Quantitatively counting plate
CN201402261Y (en) * 2009-05-11 2010-02-10 周惠玲 Micropore board
CN201791096U (en) * 2010-09-26 2011-04-13 李峻 Quick tablet counter
CN103822860A (en) * 2014-03-13 2014-05-28 河北联合大学 Method for measuring total dust number concentration in air of workplace
CN203672880U (en) * 2014-01-08 2014-06-25 陈樱 Blood counting chamber
CN204178390U (en) * 2014-11-07 2015-02-25 四川农业大学 A kind of seed counting device
CN104535760A (en) * 2014-12-31 2015-04-22 重庆科斯迈生物科技有限公司 Detecting box of chemiluminescence immunity analyzer
CN106198936A (en) * 2016-08-25 2016-12-07 刘学红 Liquid coating volume solids content measures scraper plate and using method thereof
CN206696154U (en) * 2017-04-10 2017-12-01 遵义医学院附属医院 A kind of blood counting chamber

Patent Citations (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU57672A1 (en) * 1938-11-09 1939-11-30 И.Н. Декаристо A device for counting and arranging seeds, etc.
GB1193994A (en) * 1967-07-27 1970-06-03 Vincent John Tune Improvements in or relating to a Counting Device
US4065000A (en) * 1976-06-03 1977-12-27 Gary Murton Pharmaceutical tablet and capsule counter
US4726237A (en) * 1985-06-19 1988-02-23 Sequoia-Turner Corporation Fluid metering apparatus and method
CN2175433Y (en) * 1993-11-09 1994-08-24 金华钧 Counting means for microscope
US5876604A (en) * 1996-10-24 1999-03-02 Compact Membrane Systems, Inc Method of gasifying or degasifying a liquid
US20030099577A1 (en) * 2000-04-03 2003-05-29 Philippe Renaud Device for dispensing accurately-controlled small doses of liquid
CN2435323Y (en) * 2000-08-09 2001-06-20 安文静 Hand Western medicine tablet separate packaging machine
CN2478109Y (en) * 2001-03-09 2002-02-20 赵翀 Immunity pick-up plate
CN2480842Y (en) * 2001-04-26 2002-03-06 王卫星 Uropsammus quantitative analysis instrument
CN2578829Y (en) * 2002-11-16 2003-10-08 合肥新月电子技术有限责任公司 Flow counting pool
CN101160520A (en) * 2005-04-08 2008-04-09 布勒医药股份公司 Apparatus for filling a sample volume defining device
CN200956009Y (en) * 2006-06-12 2007-10-03 河南农业大学 Device for counting eggs by microscope
CN201229288Y (en) * 2008-07-28 2009-04-29 南京千盛医疗设备制造有限公司 Quantitatively counting plate
CN201402261Y (en) * 2009-05-11 2010-02-10 周惠玲 Micropore board
CN201791096U (en) * 2010-09-26 2011-04-13 李峻 Quick tablet counter
CN203672880U (en) * 2014-01-08 2014-06-25 陈樱 Blood counting chamber
CN103822860A (en) * 2014-03-13 2014-05-28 河北联合大学 Method for measuring total dust number concentration in air of workplace
CN204178390U (en) * 2014-11-07 2015-02-25 四川农业大学 A kind of seed counting device
CN104535760A (en) * 2014-12-31 2015-04-22 重庆科斯迈生物科技有限公司 Detecting box of chemiluminescence immunity analyzer
CN106198936A (en) * 2016-08-25 2016-12-07 刘学红 Liquid coating volume solids content measures scraper plate and using method thereof
CN206696154U (en) * 2017-04-10 2017-12-01 遵义医学院附属医院 A kind of blood counting chamber

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