CN113358545A - Non-consumable cell counting sample table based on image method - Google Patents
Non-consumable cell counting sample table based on image method Download PDFInfo
- Publication number
- CN113358545A CN113358545A CN202110672154.5A CN202110672154A CN113358545A CN 113358545 A CN113358545 A CN 113358545A CN 202110672154 A CN202110672154 A CN 202110672154A CN 113358545 A CN113358545 A CN 113358545A
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- sample
- sample stage
- image method
- consumable
- cover glass
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- 238000000034 method Methods 0.000 title claims abstract description 35
- 239000010453 quartz Substances 0.000 claims abstract description 24
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000006059 cover glass Substances 0.000 claims abstract description 20
- 239000002699 waste material Substances 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 4
- 229910052594 sapphire Inorganic materials 0.000 claims description 4
- 239000010980 sapphire Substances 0.000 claims description 4
- 238000004026 adhesive bonding Methods 0.000 claims description 2
- 238000007599 discharging Methods 0.000 abstract description 4
- 230000000149 penetrating effect Effects 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 description 11
- 229920003023 plastic Polymers 0.000 description 7
- 239000004033 plastic Substances 0.000 description 7
- 239000011521 glass Substances 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G01N2015/1022—
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- G01N2015/1024—
Abstract
The invention relates to the technical field of cell counting, in particular to a consumable-free cell counting sample table based on an image method, which comprises a sample table base, wherein a plurality of grooves are formed in the top end of the sample table base at equal intervals along the length direction, quartz cover glass is arranged at the top of the sample table base, a plurality of sample cavities are formed between the sample table base and the quartz cover glass, a sample hole penetrating through the quartz cover glass is formed in one end, located on each groove, of the quartz cover glass, a waste cell sample liquid discharging port penetrating through the sample table base is formed in one end, far away from each sample hole, of each groove, a hollow cylinder is arranged in each waste cell sample liquid discharging port, and the bottom end of each hollow cylinder is connected with a hose.
Description
Technical Field
The invention relates to the technical field of cell counting, in particular to a consumable-free cell counting sample table based on an image method.
Background
Cells become conventional research objects in industries such as basic scientific research, medical clinic and pharmacy, cell counting almost becomes indispensable work, how to rapidly and accurately count the cells at low cost becomes a first problem, and at present, conventional cell counting methods in the market have two types: the image method and the coulter method operate as follows:
1. an image method: after staining cells, a researcher adds the stained cells into a standard glass counting plate or a disposable plastic counting plate, places the counting plate into a cell counting instrument for photographing, and accurately judges the death and the activity of the cells according to the staining condition of the cells in the photograph, thereby respectively calculating the concentration and the total number of the dead and the active cells. The method is high in speed and detection efficiency, but after the method is used every time, the disposable plastic cell counting plate is directly discarded, white plastic pollution is easily caused, the use cost is high, if the glass counting plate is used, the glass counting plate needs to be cleaned and dried for later use, a large amount of time is needed, and the counting efficiency is low.
2. The coulter method: researchers have processed cell samples into single cell suspensions and added the cell suspensions to capillary or microfluidic chips. The size of the cells is judged by the strength of the electric signal and the number of the cells is counted. However, after each counting is completed, a lot of time is spent on cleaning the capillary and the microfluidic chip, and the capillary is easily blocked due to poor preparation of cell suspension. The coulter method has accurate counting, but the counting time is long, the requirement of rapid counting of dozens of even hundreds of cell samples cannot be met, and the low efficiency is an unavoidable problem.
In order to solve the problems of high use cost and white pollution of disposable plastic consumables of the current image method and the defect of low technical efficiency of the coulter method, a consumable cell counting sample table which can be repeatedly used, is self-cleaning, has quick, accurate and low cost cell counting and is based on the image method is needed to improve the problems.
Disclosure of Invention
The invention aims to provide a non-consumable cell counting sample stage based on an image method, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a no consumptive material cell count sample platform based on image method, includes sample stage base, a plurality of recesses have been seted up along the length direction equidistance in the top of sample stage base, sample stage base top is provided with quartz cover glass, form a plurality of sample chambeies between sample stage base and the quartz cover glass, the sample hole that link up quartz cover glass is seted up to the one end that lies in the recess on the quartz cover glass, the waste cell sample leakage fluid dram that link up sample stage base is seted up to the one end that the sample hole was kept away from to the recess, be provided with hollow cylinder in the waste cell sample leakage fluid dram, the hollow cylinder bottom is connected with the hose.
As a preferable scheme of the invention, one end of the hose, which is far away from the hollow cylinder, is connected with the multi-channel electromagnetic valve and then is connected with the electric vacuum pump device.
As a preferable scheme of the invention, the surface of the sample stage base is subjected to plasma hydrophilic treatment, and the sample stage base is made of high-hardness and high-wear-resistance quartz/sapphire material.
In a preferred embodiment of the present invention, the diameter of the waste cell sample drain port is equal to the width of the groove, the outer diameter of the hollow cylinder is equal to the diameter of the waste cell sample drain port, and the hollow cylinder and the sample stage base are bonded or adhesively bonded to each other.
As a preferable scheme of the invention, the distance between the centers of two adjacent grooves is 9 mm.
As a preferable scheme of the invention, the cross section area of the quartz cover glass is the same as or smaller than that of the sample stage base, the cross section of the sample hole is in a circular arc shape, and the diameter of the sample hole is the same as the width of the groove.
As a preferable scheme of the invention, the depth of the groove ranges from 10um to 500 um.
Compared with the prior art, the invention has the beneficial effects that:
in the invention, the cell sample is added into the sample hole, the cell uniformly enters the sample cavity, the counting is carried out by taking a picture by combining with a cell counting instrument of an image method, the rapid and accurate counting can be realized, after the counting is finished, the electromagnetic valve is opened, the cell sample is pumped out of the sample cavity by the electric vacuum pump device through the hollow round body connected at the lower part of the sample cavity, no residual cell sample exists in the sample cavity under the action of vacuum negative pressure, at the moment, the sample can be continuously added for detection, then the detected cell sample is pumped out to ensure that the sample cavity is clean, so that a new sample can be added for detection, the operation is repeated, a disposable cell counting plate is not needed, the counting process of each cell sample is finished within 10 seconds, the number of grooves can be 1 to 96, the sample can be added by a standard 8/12 pipettor more pipettors, the detection of 1 or more samples is realized, greatly saves the operation time, improves the efficiency, and avoids the plastic pollution and the cost waste of the disposable cell counting plate.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a non-consumable cell counting sample stage based on an image method according to the present invention;
FIG. 2 is a top view of a non-consumable cell counting sample stage based on an image method according to the present invention;
FIG. 3 is a side view of a non-consumable cell counting sample stage based on an image method according to the present invention;
FIG. 4 is a schematic diagram of a sample stage base structure of a non-consumable cell counting sample stage based on an image method.
In the figure: 1. a sample stage base; 101. a groove; 102. a waste cell sample liquid outlet; 2. quartz cover glass; 201. a sample well; 3. a hollow cylinder; 301. a hose.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Several embodiments of the invention are presented in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-4, the present invention provides a technical solution:
a consumable-free cell counting sample stage based on an image method comprises a sample stage base 1, wherein a plurality of grooves 101 are formed in the top end of the sample stage base 1 at equal intervals along the length direction, a quartz cover glass 2 is arranged at the top of the sample stage base 1, a plurality of sample cavities are formed between the sample stage base 1 and the quartz cover glass 2, a sample hole 201 penetrating through the quartz cover glass 2 is formed in one end, located on the groove 101, of the quartz cover glass 2, a waste cell sample liquid discharging port 102 penetrating through the sample stage base 1 is formed in one end, away from the sample hole 201, of the groove 101, a hollow cylinder 3 is arranged in the waste cell sample liquid discharging port 102, a hose 301 is connected to the bottom end of the hollow cylinder 3, a multi-channel electromagnetic valve is commonly connected to one end, away from the hollow cylinder 3, of the hose 301, and then an electric vacuum pump device is connected, in the invention, cells are uniformly fed into the sample cavities by adding the cell samples into the sample hole 201, the counting is carried out by taking a picture in combination with an image method cell counting instrument, so that the rapid and accurate counting can be realized, the electromagnetic valve is opened, the electric vacuum pump device works to pump the cell sample out of the sample cavity through the hollow round body 3 connected with the lower part of the sample cavity, under the action of vacuum negative pressure, no residual cell sample exists in the sample cavity, at the moment, the sample can be continuously added for detection, then the cell sample after detection is pumped away to ensure that the sample cavity is clean, so that a new sample can be added for detection, the operation is repeated, a disposable cell counting plate is not needed, and each cell sample counting process is completed within 10 seconds, and the number of the grooves 101 may be 1 to 96, with the application of sample of standard 8/12 way pipettor, realize the detection of 1 or a plurality of samples, save operating time greatly, raise the efficiency, avoided the plastics pollution and the cost waste of disposable cell count board.
In an embodiment, referring to fig. 1, fig. 2, fig. 3 and fig. 4, the surface of the sample stage base 1 is subjected to plasma hydrophilic treatment, so that a cell sample can easily enter a hydrophilic micron-sized sample chamber and can be repeatedly used without abrasion or scratch, and the sample stage base 1 is made of high-hardness and high-wear-resistance quartz/sapphire material, which may be quartz, sapphire, high-hardness glass, polydimethylsiloxane, polymethyl methacrylate, or the like.
In an embodiment, referring to fig. 1 and fig. 3, a diameter of the waste cell sample liquid discharge port 102 is the same as a width of the groove 101, an outer diameter of the hollow cylinder 3 is the same as the diameter of the waste cell sample liquid discharge port 102, the hollow cylinder 3 and the sample stage base 1 are adhered by gluing, and the hollow cylinder 3 and the sample stage base 1 can be fastened by bonding or screwing, and only needs to be connected and fastened without leakage.
In the embodiment, referring to fig. 1, fig. 2 and fig. 4, the center distance between two adjacent grooves 101 is 9mm, the cross-sectional area of the quartz cover glass 2 is the same as or smaller than that of the base 1 of the sample stage, the cross-section of the sample hole 201 is arc-shaped, and the diameter of the sample hole 201 is the same as the width of the groove 101.
In one embodiment, referring to fig. 1, 3 and 4, the depth of the groove 101 ranges from 10um to 500um, such as 30um, 50um, 100um,200um, 300um, etc., and different depths of the sample wells are selected according to different cell sample concentrations.
The working principle of the invention is as follows: when the cell sample counting device is used, a cell sample is added into a sample hole 201, the cell uniformly enters a sample cavity, an image method cell counting instrument is combined, counting is carried out by photographing, rapid and accurate counting can be realized, after the counting is finished, an electromagnetic valve is opened, an electric vacuum pump device works to pump the cell sample out of the sample cavity through a hollow round body 3 connected to the lower part of the sample cavity, no residual cell sample exists in the sample cavity under the action of vacuum negative pressure, at the moment, the sample can be continuously added for detection, then the detected cell sample is pumped out to ensure that the sample cavity is clean, so that a new sample can be added for detection, the operation is repeated, a disposable cell counting plate is not needed, the counting process of each cell sample is finished within 10 seconds, the number of the grooves 101 can be 1 to 96, the sample can be added by using a standard 8/12 pipette, and the detection of 1 or more samples can be realized, greatly saves the operation time, improves the efficiency, and avoids the plastic pollution and the cost waste of the disposable cell counting plate.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a no consumptive material cell count sample platform based on image method, includes sample platform base (1), its characterized in that: a plurality of recesses (101) have been seted up along length direction equidistance to the top of sample stage base (1), sample stage base (1) top is provided with quartz cover glass (2), form a plurality of sample chambeies between sample stage base (1) and quartz cover glass (2), sample hole (201) that link up quartz cover glass (2) are seted up to the one end that lies in recess (101) on quartz cover glass (2), waste cell sample leakage fluid dram (102) that link up sample stage base (1) is seted up to the one end that sample hole (201) were kept away from in recess (101), be provided with hollow cylinder (3) in waste cell sample leakage fluid dram (102), hollow cylinder (3) bottom is connected with hose (301).
2. The non-consumable cytometric sample stage based on image method of claim 1, wherein: one end of the hose (301) far away from the hollow cylinder (3) is connected with a multi-channel electromagnetic valve and then connected with an electric vacuum pump device.
3. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the surface of the sample stage base (1) is subjected to plasma hydrophilic treatment, and the sample stage base (1) is made of high-hardness and high-wear-resistance quartz/sapphire materials.
4. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the diameter of the waste cell sample liquid outlet (102) is the same as the width of the groove (101), the outer diameter of the hollow cylinder (3) is the same as the diameter of the waste cell sample liquid outlet (102), and the hollow cylinder (3) is bonded or adhered to the sample stage base (1) through bonding or gluing.
5. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the distance between the centers of two adjacent grooves (101) is 9 mm.
6. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the cross section area of the quartz cover glass (2) is the same as that of the sample table base (1) or smaller than that of the sample table base (1), the cross section of the sample hole (201) is arc-shaped, and the diameter of the sample hole (201) is the same as that of the groove (101).
7. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the depth range of the groove (101) is 10um-500 um.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110672154.5A CN113358545A (en) | 2021-06-17 | 2021-06-17 | Non-consumable cell counting sample table based on image method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110672154.5A CN113358545A (en) | 2021-06-17 | 2021-06-17 | Non-consumable cell counting sample table based on image method |
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| Publication Number | Publication Date |
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| CN113358545A true CN113358545A (en) | 2021-09-07 |
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| CN202110672154.5A Pending CN113358545A (en) | 2021-06-17 | 2021-06-17 | Non-consumable cell counting sample table based on image method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114839133A (en) * | 2022-04-06 | 2022-08-02 | 高分(北京)生物科技有限公司 | Cell imaging counting device |
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2021
- 2021-06-17 CN CN202110672154.5A patent/CN113358545A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114839133A (en) * | 2022-04-06 | 2022-08-02 | 高分(北京)生物科技有限公司 | Cell imaging counting device |
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