CN215263037U

CN215263037U - Non-consumable cell counting sample table based on image method

Info

Publication number: CN215263037U
Application number: CN202121345869.1U
Authority: CN
Inventors: 蔡应辉
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-06-17
Filing date: 2021-06-17
Publication date: 2021-12-21
Anticipated expiration: 2031-06-17

Abstract

The utility model belongs to the technical field of the cell count technique and specifically relates to a no consumptive material cell count sample platform based on image method, including the sample stage base, a plurality of recesses have been seted up along the length direction equidistance in the top of sample stage base, sample stage base top is provided with quartz cover glass, form a plurality of sample chambers between sample stage base and the quartz cover glass, the sample hole that link up quartz cover glass is seted up to the one end that lies in the recess on the quartz cover glass, the waste cell sample leakage fluid dram that link up the sample stage base is seted up to the one end that the sample hole was kept away from to the recess, be provided with hollow cylinder in the waste cell sample leakage fluid dram, the hollow cylinder bottom is connected with the hose, the utility model discloses save operating time greatly, raise the efficiency, avoided the plastics pollution and the cost waste of disposable cell count board.

Description

Non-consumable cell counting sample table based on image method

Technical Field

The utility model relates to a cell count technical field specifically is a no consumptive material cell count sample platform based on image method.

Background

Cells become conventional research objects in industries such as basic scientific research, medical clinic and pharmacy, cell counting almost becomes indispensable work, how to rapidly and accurately count the cells at low cost becomes a first problem, and at present, conventional cell counting methods in the market have two types: the image method and the coulter method operate as follows:

1. an image method: after staining cells, a researcher adds the stained cells into a standard glass counting plate or a disposable plastic counting plate, places the counting plate into a cell counting instrument for photographing, and accurately judges the death and the activity of the cells according to the staining condition of the cells in the photograph, thereby respectively calculating the concentration and the total number of the dead and the active cells. The method is high in speed and detection efficiency, but after the method is used every time, the disposable plastic cell counting plate is directly discarded, white plastic pollution is easily caused, the use cost is high, if the glass counting plate is used, the glass counting plate needs to be cleaned and dried for later use, a large amount of time is needed, and the counting efficiency is low.

2. The coulter method: researchers have processed cell samples into single cell suspensions and added the cell suspensions to capillary or microfluidic chips. The size of the cells is judged by the strength of the electric signal and the number of the cells is counted. However, after each counting is completed, a lot of time is spent on cleaning the capillary and the microfluidic chip, and the capillary is easily blocked due to poor preparation of cell suspension. The coulter method has accurate counting, but the counting time is long, the requirement of rapid counting of dozens of even hundreds of cell samples cannot be met, and the low efficiency is an unavoidable problem.

In order to solve the problems of high use cost and white pollution of disposable plastic consumables of the current image method and the defect of low technical efficiency of the coulter method, a consumable cell counting sample table which can be repeatedly used, is self-cleaning, has quick, accurate and low cost cell counting and is based on the image method is needed to improve the problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a no consumptive material cell count sample platform based on image method to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

the utility model provides a no consumptive material cell count sample platform based on image method, includes sample stage base, a plurality of recesses have been seted up along the length direction equidistance in the top of sample stage base, sample stage base top is provided with quartz cover glass, form a plurality of sample chambeies between sample stage base and the quartz cover glass, the sample hole that link up quartz cover glass is seted up to the one end that lies in the recess on the quartz cover glass, the waste cell sample leakage fluid dram that link up sample stage base is seted up to the one end that the sample hole was kept away from to the recess, be provided with hollow cylinder in the waste cell sample leakage fluid dram, the hollow cylinder bottom is connected with the hose.

As the utility model discloses a preferred scheme, the one end that hollow cylinder was kept away from to the hose is connected with the multichannel solenoid valve jointly and connects the electric vacuum pump device again.

As the utility model discloses an optimal scheme, the surface of sample platform base is through plasma hydrophilic treatment, sample platform base adopts the high wear-resisting quartzy sapphire material of high rigidity.

As the preferred embodiment of the present invention, the diameter of the waste cell sample liquid outlet is the same as the width of the groove, the outer diameter of the hollow cylinder is the same as the diameter of the waste cell sample liquid outlet, and the hollow cylinder and the sample stage base are bonded or adhesively bonded to each other.

As the preferred scheme of the utility model, adjacent two the central distance of recess is 9 mm.

As the preferred scheme of the utility model, the cross-sectional area of quartz cover glass is the same with the cross-sectional area of sample platform base or is less than the cross-sectional area of sample platform base, the cross-section of sample hole is convex, and the diameter of sample hole is the same with the width of recess.

As the preferred scheme of the utility model, the degree of depth scope of recess is 10um-500 um.

Compared with the prior art, the beneficial effects of the utility model are that:

in the utility model, the cell sample is added into the sample hole, the cell uniformly enters the sample cavity, the cell counter is combined with the image method, the cell sample is counted by taking a picture, the rapid and accurate counting can be realized, after the counting is finished, the electromagnetic valve is opened, the cell sample is pumped out from the sample cavity by the hollow round body connected at the lower part of the sample cavity by the electric vacuum pump device, no residual cell sample exists in the sample cavity under the action of vacuum negative pressure, at the moment, the sample can be continuously added for detection, then the detected cell sample is pumped out to ensure the cleanness of the sample cavity, so that a new sample can be added for detection, the repeated operation is carried out, a disposable cell counting plate is not needed, in addition, the counting process of each cell sample is completed within 10 seconds, the number of the grooves can be 1 to 96, the sample can be added by a standard pipettor 8/12 sample adding devices, the detection of 1 or more samples can be realized, greatly saves the operation time, improves the efficiency, and avoids the plastic pollution and the cost waste of the disposable cell counting plate.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a cell counting sample stage without consumable materials based on an image method according to the present invention;

FIG. 2 is a top view of a cell counting sample stage without consumable materials based on an image method according to the present invention;

FIG. 3 is a side view of a cell counting sample stage without consumable materials based on an image method according to the present invention;

fig. 4 is the utility model relates to a sample platform base structure sketch map of no consumptive material cell count sample platform based on image method.

In the figure: 1. a sample stage base; 101. a groove; 102. a waste cell sample liquid outlet; 2. quartz cover glass; 201. a sample well; 3. a hollow cylinder; 301. a hose.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. Several embodiments of the invention are given in the accompanying drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1-4, the present invention provides a technical solution:

a consumable-free cell counting sample stage based on an image method comprises a sample stage base 1, a plurality of grooves 101 are equidistantly formed in the top end of the sample stage base 1 along the length direction, a quartz cover glass 2 is arranged at the top of the sample stage base 1, a plurality of sample cavities are formed between the sample stage base 1 and the quartz cover glass 2, a sample hole 201 penetrating through the quartz cover glass 2 is formed in one end, located on the groove 101, of the quartz cover glass 2, a waste cell sample liquid discharge port 102 penetrating through the sample stage base 1 is formed in one end, far away from the sample hole 201, of the groove 101, a hollow cylinder 3 is arranged in the waste cell sample liquid discharge port 102, a hose 301 is connected to the bottom end of the hollow cylinder 3, one end, far away from the hollow cylinder 3, of the hose 301 is commonly connected with a multi-channel electromagnetic valve to be connected with an electric vacuum pump device, in the utility model, cells are uniformly introduced into the sample cavities through cell samples added into the sample holes 201, the counting is carried out by taking a picture in combination with an image method cell counting instrument, so that the rapid and accurate counting can be realized, the electromagnetic valve is opened, the electric vacuum pump device works to pump the cell sample out of the sample cavity through the hollow round body 3 connected with the lower part of the sample cavity, under the action of vacuum negative pressure, no residual cell sample exists in the sample cavity, at the moment, the sample can be continuously added for detection, then the cell sample after detection is pumped away to ensure that the sample cavity is clean, so that a new sample can be added for detection, the operation is repeated, a disposable cell counting plate is not needed, and each cell sample counting process is completed within 10 seconds, and the number of the grooves 101 may be 1 to 96, with the application of sample of standard 8/12 way pipettor, realize the detection of 1 or a plurality of samples, save operating time greatly, raise the efficiency, avoided the plastics pollution and the cost waste of disposable cell count board.

In an embodiment, referring to fig. 1, fig. 2, fig. 3 and fig. 4, the surface of the sample stage base 1 is subjected to plasma hydrophilic treatment, so that a cell sample can easily enter a hydrophilic micron-sized sample chamber and can be repeatedly used without abrasion or scratch, and the sample stage base 1 is made of high-hardness and high-wear-resistance quartz/sapphire material, which may be quartz, sapphire, high-hardness glass, polydimethylsiloxane, polymethyl methacrylate, or the like.

In an embodiment, referring to fig. 1 and fig. 3, a diameter of the waste cell sample liquid discharge port 102 is the same as a width of the groove 101, an outer diameter of the hollow cylinder 3 is the same as the diameter of the waste cell sample liquid discharge port 102, the hollow cylinder 3 and the sample stage base 1 are adhered by gluing, and the hollow cylinder 3 and the sample stage base 1 can be fastened by bonding or screwing, and only needs to be connected and fastened without leakage.

In the embodiment, referring to fig. 1, fig. 2 and fig. 4, the center distance between two adjacent grooves 101 is 9mm, the cross-sectional area of the quartz cover glass 2 is the same as or smaller than that of the base 1 of the sample stage, the cross-section of the sample hole 201 is arc-shaped, and the diameter of the sample hole 201 is the same as the width of the groove 101.

In one embodiment, referring to fig. 1, 3 and 4, the depth of the groove 101 ranges from 10um to 500um, such as 30um, 50um, 100um,200um, 300um, etc., and different depths of the sample wells are selected according to different cell sample concentrations.

The utility model discloses the theory of operation: when the cell sample counting device is used, a cell sample is added into a sample hole 201, the cell uniformly enters a sample cavity, an image method cell counting instrument is combined, counting is carried out by photographing, rapid and accurate counting can be realized, after the counting is finished, an electromagnetic valve is opened, an electric vacuum pump device works to pump the cell sample out of the sample cavity through a hollow round body 3 connected to the lower part of the sample cavity, no residual cell sample exists in the sample cavity under the action of vacuum negative pressure, at the moment, the sample can be continuously added for detection, then the detected cell sample is pumped out to ensure that the sample cavity is clean, so that a new sample can be added for detection, the operation is repeated, a disposable cell counting plate is not needed, the counting process of each cell sample is finished within 10 seconds, the number of the grooves 101 can be 1 to 96, the sample can be added by using a standard 8/12 pipette, and the detection of 1 or more samples can be realized, greatly saves the operation time, improves the efficiency, and avoids the plastic pollution and the cost waste of the disposable cell counting plate.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a no consumptive material cell count sample platform based on image method, includes sample platform base (1), its characterized in that: a plurality of recesses (101) have been seted up along length direction equidistance to the top of sample stage base (1), sample stage base (1) top is provided with quartz cover glass (2), form a plurality of sample chambeies between sample stage base (1) and quartz cover glass (2), sample hole (201) that link up quartz cover glass (2) are seted up to the one end that lies in recess (101) on quartz cover glass (2), waste cell sample leakage fluid dram (102) that link up sample stage base (1) is seted up to the one end that sample hole (201) were kept away from in recess (101), be provided with hollow cylinder (3) in waste cell sample leakage fluid dram (102), hollow cylinder (3) bottom is connected with hose (301).

2. The non-consumable cytometric sample stage based on image method of claim 1, wherein: one end of the hose (301) far away from the hollow cylinder (3) is connected with a multi-channel electromagnetic valve and then connected with an electric vacuum pump device.

3. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the surface of the sample stage base (1) is subjected to plasma hydrophilic treatment, and the sample stage base (1) is made of high-hardness and high-wear-resistance quartz/sapphire materials.

4. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the diameter of the waste cell sample liquid outlet (102) is the same as the width of the groove (101), the outer diameter of the hollow cylinder (3) is the same as the diameter of the waste cell sample liquid outlet (102), and the hollow cylinder (3) is bonded or adhered to the sample stage base (1) through bonding or gluing.

5. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the distance between the centers of two adjacent grooves (101) is 9 mm.

6. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the cross section area of the quartz cover glass (2) is the same as that of the sample table base (1) or smaller than that of the sample table base (1), the cross section of the sample hole (201) is arc-shaped, and the diameter of the sample hole (201) is the same as that of the groove (101).

7. The non-consumable cytometric sample stage based on image method of claim 1, wherein: the depth range of the groove (101) is 10um-500 um.