CN107167603A - Circulating tumor cell assay instrument - Google Patents
Circulating tumor cell assay instrument Download PDFInfo
- Publication number
- CN107167603A CN107167603A CN201610969979.2A CN201610969979A CN107167603A CN 107167603 A CN107167603 A CN 107167603A CN 201610969979 A CN201610969979 A CN 201610969979A CN 107167603 A CN107167603 A CN 107167603A
- Authority
- CN
- China
- Prior art keywords
- reagent
- circulating tumor
- micro flow
- tumor cell
- pellosil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
Abstract
The present invention relates to a kind of circulating tumor cell assay instrument, including main frame, liquid control system, sample cell, micro flow chip, waste liquid bottle and pipeline, reagent base and chip coupler are provided with the main frame, it is placed with the reagent base in Reagent Tube, the chip coupler and is placed with micro flow chip；The liquid control system is connected with Reagent Tube, sample cell and waste liquid bottle respectively by pipeline, and the sample cell is connected with micro flow chip, and the micro flow chip is connected by pipeline with waste liquid bottle；Described micro flow chip includes upper lid, lower cover, pellosil and slide, and described pellosil is fitted on slide, is placed between lid and lower cover；The one side of the pellosil laminating slide is provided with path.Fluid present invention control system and micro flow chip are used in combination, and the agents in combination of the pellosil with the Pacific herring fish bone well structure having levels and circulation rare cell specific binding is used, and substantially increases the capture rate of circulation rare cell.
Description
Technical field
The present invention relates to a kind of circulating tumor cell assay instrument, the sorting of circulation rare cell is primarily adapted for use in, belongs to body
Outer diagnostic techniques field.
Background technology
Circulating tumor cell assay instrument is a kind of non-invasive system, rare for capturing, counting, separate and collecting circulation
Cell（Circulating Rare Cells, CRCs）.Translational medicine is studied and the circulation rare cell of clinical research includes three
Individual Main Subtype, is circulating tumor cell respectively（Circulating Tumor Cells, CTCs）, circulating stem cell
（Circulating Stem Cells, CSCs）, and circulation fetal cell（Circulating Fetal Cells,CFCs）.
By standardization, GMP grades of biological reagent separation circulation rare cell, the CRCs of high-purity and high activity can be so obtained, is used
In downstream Characterisation of proteins, genetic analysis and cell analysis.Effective unicellular separation and collection are CRCs in diagnosis and pharmacy work
Industry opens new application.
The content of the invention
It is an object of the invention to provide a kind of reasonable in design, capture rate be high, good separation effect circulating tumor
Cytoanalyze.
The present invention the used technical scheme that solves the above problems is：The circulating tumor cell assay instrument includes main frame, liquid
Body control system, sample cell, micro flow chip, waste liquid bottle and pipeline, it is characterised in that：Reagent base is provided with described main frame
With chip coupler, it is placed with Reagent Tube, described chip coupler on the reagent base and is placed with micro flow chip；It is described
Main frame on be provided with liquid control system, described liquid control system by pipeline respectively with Reagent Tube, sample cell and useless
Liquid bottle is connected, and described sample cell be connected and is interconnected with micro flow chip, and described micro flow chip is by pipeline and gives up
Liquid bottle connection；Described micro flow chip includes upper lid, lower cover, pellosil and slide, and described pellosil is fitted on slide, institute
The pellosil and slide stated are placed between lid and lower cover；The one side of the pellosil laminating slide is provided with path, path
It is the sunk structure on pellosil.It is coated with and can and circulates rare in the one side of slide and the pellosil laminating of the micro flow chip
The material that cell-specific is combined, for capturing circulation rare cell, mainly circulating tumor cell.
Liquid control system of the present invention includes reagent transfer valve, loading valve, waste liquid dump valve, air bleeding valve and injection
Pump；Described reagent transfer valve is connected by pipeline with Reagent Tube, and described loading valve is connected by pipeline with sample cell, described
Waste liquid dump valve be connected by pipeline with waste liquid bottle, described air bleeding valve is communicated with extraneous air；Described syringe pump with it is upper
State four kinds of valve connections.Liquid control system improves the automaticity of whole instrument, convenient cleaning and sample detection.
Preferred described reagent transfer valve at least two of the invention, described Reagent Tube at least two.Reagent Tube and
The quantity of transfer valve can be changed according to using the species and composition of reagent, but at least need to set a kind of cleaning reagent and one kind
Detection reagent, therefore Reagent Tube and reagent transfer valve are at least provided with two.Can be a Reagent Tube correspondence in practical application
One reagent transfer valve or multiple Reagent Tubes one reagent transfer valve of correspondence；When multiple Reagent Tubes one reagent of correspondence
During transfer valve, actual switching can be realized by another sleeving valve between multiple Reagent Tubes.
Preferred at least two described, path of the invention.Described path is preferably one and is divided into two, by two points
Be divided into eight for four, by four, totally eight paths, sample solution is shunted in path, fully with slide with circulate it is dilute
There is the material contact that cell-specific is combined, improve circulation rare cell capture rate.
There are multiple projections in path of the present invention, the raised height is less than the depth in path.Projection makes sample
Resistance is added when flowing through path, but does not influence sample solution flow forward.
Raised height of the present invention gradually increases according to liquid flow direction.The structure having levels can reach gradient filtration
Effect, reduce the loss of the larger target cell of volume.Cell mass or bulky cell can be blocked step by step, and be circulated
The volume of rare cell is more than normal blood cell, and can form cell mass, and such structure is beneficial to normal blood cell
Pass through, stayed in while circulation rare cell is intercepted in micro flow chip, and assisted with the material of circulation rare cell specific binding
Same-action, improves circulation rare cell capture rate.
It is of the present invention multiple raised in herringbone, and direction is consistent, spacing is equal, in regularly arranged.Rule row
The herringbone projection of row collectively forms Pacific herring fish bone well structure, beneficial to sample solution Rolling flow, fully dilute with circulation on slide
There is the contact of cell capture material.
Chip coupler of the present invention is provided with temperature control system.When temperature control system is adjustably controlled reaction
Temperature, be conducive to the successful capture and complete release of cell.Described temperature control system is usually in chip coupler
Bottom sets the heating plate of one piece of temperature-adjustable.
Ventilating fan is provided with main frame of the present invention.Ventilating fan operating can help main frame to radiate, so as to instrument just
Often operation.
Digital display screen is provided with main frame of the present invention.Digital display screen can displays temperature and voltage, be easy to course of reaction
The control of temperature and flow rate of liquid.
Pipeline aperture of the present invention is very tiny, forms miniflow, improves cell capture efficiency.
The present invention has advantages below and effect：Liquid control system and micro flow chip are used in combination, with what is had levels
The pellosil of Pacific herring fish bone well structure and the agents in combination of circulation rare cell specific binding are used, and substantially increase circulation rare
The capture rate of cell.The present invention is used for the separation and identification of rare cell in human peripheral, can obtain high-purity and active
Rare cell, characterized applied to downstream protein, genetic analysis and cell detection, joint detection results have examining for disease
The clinical assistant diagnosis effect such as disconnected, prevention, monitoring.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention.
Fig. 2 is the fractionation structural representation of micro flow chip of the present invention.
Fig. 3 is the structural representation of pellosil laminating slide one side of the present invention.
Fig. 4 is the Section A-A figure in wherein one path of Fig. 3.
In figure：Main frame 1, Reagent Tube 11, reagent base 12, chip coupler 13, ventilating fan 14, digital display screen 15；Liquid control
System 2 processed, transfer valve 21, loading valve 22, waste liquid dump valve 23, exhaust 24, syringe pump 25；Pipeline 3, sample cell 4, micro flow chip
5, waste liquid bottle 6；Pellosil 51, slide 52, upper lid 53, lower cover 54；Path 511, projection 512.
Embodiment
Below in conjunction with the accompanying drawings and the invention will be further described by embodiment.
Referring to Fig. 1, the present embodiment includes：Main frame 1, liquid control system 2, pipeline 3, sample cell 4, micro flow chip 5, and it is useless
Liquid bottle 6.
In the present embodiment, main frame 1 is provided with reagent base 12, reagent base 12 is placed with three Reagent Tubes above
11.Chip coupler 13, ventilating fan 14 and digital display screen 15 are additionally provided with main frame 1；Chip coupler 13 is used to place miniflow core
Piece 5；The operating of ventilating fan 14 can help main frame 1 to radiate, so that instrument is normally run；Digital display screen 15 can displays temperature and voltage,
It is easy to the temperature of course of reaction and the control of flow rate of liquid.
In the present embodiment, liquid control system 2 is arranged on main frame 1, and liquid control system 2 includes two transfer valves
21, a loading valve 22, a waste liquid dump valve 23, an air bleeding valve 24 and a syringe pump 25, syringe pump 25 and above-mentioned five
Individual valve connection, power is provided for liquid flowing；One of transfer valve 21 is connected with two Reagent Tubes 11 by pipeline 3, and this two
Individual Reagent Tube 11 is used to hold cleaning reagent, usually buffer solution and ethanol for disinfection；Another transfer valve 21 with remaining one
Reagent Tube 11 is connected by pipeline 3, and this Reagent Tube 11 is used to hold detection reagent, usually cell dyeing pretreating reagent, uses
In the precellular pretreatment of dyeing；Loading valve 22 is connected with sample cell 4 by pipeline 3, when cleaning and detecting, Reagent Tube 11, defeated
Enter valve 21, loading valve 22 to connect with sample cell 4, form a path for leading to micro flow chip 5；Waste liquid dump valve 23 passes through pipeline
3 are connected with waste liquid bottle 6, for discharging the waste liquid in liquid control system 2；Air bleeding valve 24 is connected with extraneous air, when liquid control
When producing bubble in system 2 processed, for discharging excessive gas, have a small amount of liquid and discharge together, wiped clean.
Referring to Fig. 2-4, in the present embodiment, micro flow chip 5 is four-layer structure, be from top to bottom upper lid 53, pellosil 51,
Slide 52 and lower cover 54, wherein, pellosil 51 is fitted in above slide 52, and upper lid 53 is covered on lower cover 54, forms a sky
Chamber, pellosil 51 and slide 52 are placed in this cavity；The one side of the laminating slide 52 of pellosil 51 is the following table of pellosil 51
Face Shang You paths 511, path 511 is to have projection 512 in the list structure of depression, path 511, and the height of projection 512 is less than river in Zhejiang Province
The depth in road 511；Pellosil 51 is PDMS（Dimethyl silicone polymer）Material, path 511 and projection 512 are added using etch process
Work is formed；The material that rare cell specific binding and can be circulated is coated with the one side of the laminating pellosil 51 of slide 52, in sample
When originally flowing through path 511, the circulation rare cell in sample, which can be captured, to be stayed in micro flow chip 5；Remaining waste liquid passes through pipe
Line 3 flows into waste liquid bottle 6.
In the present embodiment, path 511 forms eight paths altogether by one-to-two, by two point four, by four point eight；Eight lead to
Lu Zhongjun has a projection 512, and all raised 512 in herringbone structure, direction be consistent, adjacent spacing is equal, collectively forms Pacific herring
Fish bone well structure；Meanwhile, according to liquid flow direction, the height of projection 512 gradually increases, and forms a kind of Pacific herring fish bone well having levels
Structure, can reach the effect of gradient filtration, i.e. cell mass or bulky cell can be blocked step by step, and circulate rare cell
Volume is more than normal blood cell, and can form cell mass, and such structure passes through beneficial to normal blood cell, simultaneously will
Circulation rare cell, which is intercepted, to be stayed in micro flow chip 5.
The course of work of the present invention is as follows：
First, clean
Transfer valve 21, loading valve 22 and syringe pump 25 that the Reagent Tube 11 of cleaning reagent is connected are controlled and held, makes cleaning reagent
Sample cell 4 is flowed to by pipeline 3 by Reagent Tube 11, micro flow chip 5 is flowed through, waste liquid bottle 6, two examinations are flowed to finally by pipeline 3
The cleaning reagent of agent pipe can be switched by valve, and so on, complete cleaning.
2nd, detect
Loading valve 22 and syringe pump 25, separation sample cell 4 and micro flow chip 5 are controlled, sample suck-back is entered sample cell 4；Connect again
Sample cell 4 and micro flow chip 5, control loading valve 22 and syringe pump 25 are connect, sample is flowed out from sample cell 4 and slowly flows across miniflow
Chip 5, flows to waste liquid bottle 6, circulation rare cell is captured and is enriched in micro flow chip 5 finally by pipeline 3；Control is with containing
Transfer valve 21, loading valve 22 and syringe pump 25 that the Reagent Tube 11 of cell dyeing pretreating reagent is connected are put, makes to locate before cell dyeing
Reason agent flows to sample cell 4 by pipeline 3, flows through micro flow chip 5, flows to waste liquid bottle 6 finally by pipeline 3, cell dyeing is located in advance
Reason is completed；Micro flow chip 5 is taken out, lid 53 and lower cover 54, separation pellosil 51 and slide 52 is taken apart, circulation rare cell is consolidated
It is scheduled on slide 52, follow-up dyeing observation and further analysis can be carried out.
The part being not described in this specification uses structure known in those skilled in the art and principle,
For prior art.
By foregoing description, those skilled in the art can implement.
Furthermore, it is necessary to illustrate, the specific embodiment described in this specification, as long as explanation tool is not known in its part
Body specification, then the part can be any specification being adapted with its function；Meanwhile, the title that part is taken can not also
Together.The equivalent or simple change that all construction, feature and principles according to described in present inventive concept are done, is included in patent of the present invention
Protection domain in.
Claims (10)
1. a kind of circulating tumor cell assay instrument, including main frame, liquid control system, sample cell, micro flow chip, waste liquid bottle and pipe
Line, it is characterised in that：It is provided with described main frame on reagent base and chip coupler, the reagent base and is placed with reagent
Micro flow chip is placed with pipe, described chip coupler；Liquid control system, described liquid are provided with described main frame
Control system is connected with Reagent Tube, sample cell and waste liquid bottle respectively by pipeline, and described sample cell is connected with micro flow chip
And it is interconnected, described micro flow chip is connected by pipeline with waste liquid bottle；Described micro flow chip include upper lid, lower cover,
Pellosil and slide, described pellosil are fitted on slide, and described pellosil and slide is placed between lid and lower cover；
The one side of the pellosil laminating slide is provided with path, and path is the sunk structure on pellosil.
2. circulating tumor cell assay instrument according to claim 1, it is characterised in that：Described liquid control system includes
Reagent transfer valve, loading valve, waste liquid dump valve, air bleeding valve and syringe pump；Described reagent transfer valve passes through pipeline and Reagent Tube
Connection, described loading valve is connected by pipeline with sample cell, and described waste liquid dump valve is connected by pipeline with waste liquid bottle, institute
The air bleeding valve stated is communicated with extraneous air；Described syringe pump is connected with above-mentioned four kinds of valves.
3. circulating tumor cell assay instrument according to claim 2, it is characterised in that：Described reagent transfer valve is at least
Two；Described Reagent Tube at least two.
4. circulating tumor cell assay instrument according to claim 1, it is characterised in that：At least two, described path.
5. the circulating tumor cell assay instrument according to claim 1 or 4, it is characterised in that：Have multiple in described path
Projection, the raised height is less than the depth in path.
6. circulating tumor cell assay instrument according to claim 5, it is characterised in that：The raised height is according to liquid flow
Dynamic direction gradually increases.
7. circulating tumor cell assay instrument according to claim 5, it is characterised in that：Described is multiple raised in herringbone
Shape, and direction is consistent, spacing is equal, in regularly arranged.
8. circulating tumor cell assay instrument according to claim 1, it is characterised in that：Described chip coupler is provided with
Temperature control system.
9. circulating tumor cell assay instrument according to claim 1, it is characterised in that：Air draft is provided with described main frame
Fan.
10. circulating tumor cell assay instrument according to claim 1, it is characterised in that：Number is provided with described main frame
Aobvious screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610969979.2A CN107167603B (en) | 2016-11-07 | 2016-11-07 | Circulating tumor cell assay instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610969979.2A CN107167603B (en) | 2016-11-07 | 2016-11-07 | Circulating tumor cell assay instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107167603A true CN107167603A (en) | 2017-09-15 |
| CN107167603B CN107167603B (en) | 2019-09-10 |
Family
ID=59849716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610969979.2A Active CN107167603B (en) | 2016-11-07 | 2016-11-07 | Circulating tumor cell assay instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107167603B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226547A (en) * | 2017-12-22 | 2018-06-29 | 东南大学 | Circulating tumor cell detecting instrument including micro-fluidic chip |
| CN108344615A (en) * | 2018-01-24 | 2018-07-31 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems and control method |
| US20200378925A1 (en) * | 2019-05-30 | 2020-12-03 | The Regents Of The University Of California | Microfluidic Acoustic Devices and Methods |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880329A (en) * | 2005-06-13 | 2006-12-20 | 中国科学院电子学研究所 | Reversible packaging micro fluent chip for separating and purifying biological sample |
| CN205420426U (en) * | 2016-03-17 | 2016-08-03 | 杭州华得森生物技术有限公司 | Rare cell sorter circulates |
| CN205443266U (en) * | 2016-03-23 | 2016-08-10 | 杭州华得森生物技术有限公司 | Rare cell sorter's seizure system circulates |
| CN206132781U (en) * | 2016-11-07 | 2017-04-26 | 杭州华得森生物技术有限公司 | Circulating tumor cell analysis appearance |
-
2016
- 2016-11-07 CN CN201610969979.2A patent/CN107167603B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880329A (en) * | 2005-06-13 | 2006-12-20 | 中国科学院电子学研究所 | Reversible packaging micro fluent chip for separating and purifying biological sample |
| CN205420426U (en) * | 2016-03-17 | 2016-08-03 | 杭州华得森生物技术有限公司 | Rare cell sorter circulates |
| CN205443266U (en) * | 2016-03-23 | 2016-08-10 | 杭州华得森生物技术有限公司 | Rare cell sorter's seizure system circulates |
| CN206132781U (en) * | 2016-11-07 | 2017-04-26 | 杭州华得森生物技术有限公司 | Circulating tumor cell analysis appearance |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226547A (en) * | 2017-12-22 | 2018-06-29 | 东南大学 | Circulating tumor cell detecting instrument including micro-fluidic chip |
| CN108344615A (en) * | 2018-01-24 | 2018-07-31 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems and control method |
| CN108344615B (en) * | 2018-01-24 | 2020-10-23 | 杭州安弼晟生物科技有限公司 | Circulating tumor cell capturing system and control method |
| US20200378925A1 (en) * | 2019-05-30 | 2020-12-03 | The Regents Of The University Of California | Microfluidic Acoustic Devices and Methods |
| US11668676B2 (en) * | 2019-05-30 | 2023-06-06 | The Regents Of The University Of California | Microfluidic acoustic devices and methods |
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| Publication number | Publication date |
|---|---|
| CN107167603B (en) | 2019-09-10 |
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