CN205420426U - Rare cell sorter circulates - Google Patents
Rare cell sorter circulates Download PDFInfo
- Publication number
- CN205420426U CN205420426U CN201620205301.2U CN201620205301U CN205420426U CN 205420426 U CN205420426 U CN 205420426U CN 201620205301 U CN201620205301 U CN 201620205301U CN 205420426 U CN205420426 U CN 205420426U
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- chip
- rare cell
- valve
- main frame
- cell sorter
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Abstract
The utility model relates to a rare cell sorter circulates, including host computer and computer, its characterized in that: be provided with the reagent tube socket above the host computer, placed the reagent pipe on the reagent tube socket, the host computer is preceding to be provided with valve and miniature syringe pump, and the valve has four at least, and one of them valve is connected with the gas vent, and remaining valve is connected with reagent pipe, sample room and waste liquid jar through the pipeline respectively, the host computer base on be provided with chip acceptor and waste liquid jar, be connected with the sample room on the chip acceptor, placed the chip in the chip acceptor, the chip sample room is connected, the chip receiver adopts the pipeline to be connected with the waste liquid jar. The utility model discloses a little fluidic system, temperature control system, nano chips jointly use for the separation and the appraisal of rare cell in people's peripheral blood can obtain high -purity and the activated rare cell of utensil.
Description
Technical field
This utility model relates to a kind of circulation rare cell sorter, is primarily adapted for use in the sorting of rare cell, belongs to vitro diagnostic techniques field.
Background technology
Circulation rare cell sorter is a kind of non-invasive system, is used for catching, count, separate and collect circulation rare cell (CirculatingRareCells, CRCs).The circulation rare cell of translational medicine research and clinical research includes three Main Subtypes, it is circulating tumor cell (CirculatingTumorCells respectively, CTCs), circulating stem cell (CirculatingStemCells, CSCs), and circulation fetal cell (CirculatingFetalCells, CFCs).Separate circulation rare cell by the biological reagent of standardization, GMP level, so can obtain high-purity and highly active CRCs, for downstream Characterisation of proteins, gene analysis and cell analysis.Effective unicellular separation and collection, open new range of application for CRCs in diagnosis and pharmaceuticals industry.
Utility model content
The purpose of this utility model is to provide a kind of reasonable in design, capture rate height, the circulation rare cell sorter of good separation effect.
The technical scheme in the invention for solving the above technical problem is: this circulation rare cell sorter includes: main frame and computer, described main frame and computer are connected by data wire, it is characterized in that: described main frame is provided above with reagent base, and reagent base is placed with Reagent Tube；Being provided with valve and Miniature injection pump before described main frame, described valve is connected with Miniature injection pump；At least four, described valve, one of them valve connects air vent, the gas that air vent is unnecessary in discharge system, and remaining valve is connected with Reagent Tube, sample room and waste liquid cylinder by pipeline respectively；Chip accepter and waste liquid cylinder it is provided with on described main machine base, connect on described chip accepter and have sample room, being placed with chip in described chip accepter, described chip is connected with sample room, and described chip receiver uses pipeline to be connected with waste liquid cylinder.Described chip accepter can be used for fixed chip.Described chip has two kinds of forms: a kind of is blank chip during cleaning, is not coated；A kind of be detection time use nano chips, chip is coated with capture antibody, is used for catching cell.Described sample room is internal is helical structure, and helical structure enables cell fully to break up, and follow-up reacts with nano chips.
Valve described in the utility model has eight, and is all connected with same Miniature injection pump；Described Reagent Tube has five, and described five in eight valves are connected by pipeline with five described Reagent Tubes respectively.Eight valves constitute one eight, and to valve, and Miniature injection pump collectively constitutes eight to valve system, improves the automaticity of whole instrument, convenient cleans and sample-adding detection.
Chip accepter described in the utility model is with temperature control system.Temperature control system realizes by arranging thermal control plate in chip accepter bottom surface, is adjustably controlled the successful capture of temperature during reaction, beneficially rare cell and complete release.
Circulation rare cell sorter described in the utility model, it is characterised in that: it is stamped bacteriological filtration lid on described Reagent Tube.Bacteriological filtration lid can prevent the reagent in Reagent Tube to be contaminated.
Circulation rare cell sorter described in the utility model, it is characterised in that: it is provided with exhaust fan on described main frame.Exhaust fan operating can help main frame to dispel the heat, in order to instrument is properly functioning.
It is provided with digital display screen on main frame described in the utility model.Digital display screen can displays temperature and voltage, it is simple to the temperature of course of reaction and the control of flow rate of liquid.
It is provided with barcode scanner on main frame described in the utility model.Barcode scanner can the bar code of scanned samples, chip, sample room or reagent etc., facilitate late detection result computer data to arrange.
It is provided with relay indicating light on main frame described in the utility model.Relay indicating light lights and may indicate that opening of device, extinguishes then equipment and closes.
Pipeline aperture described in the utility model is the most tiny, forms miniflow, improves cell capture efficiency.
Described in the utility model eight form a microfluidic system to valve, Miniature injection pump, pipeline, sample room and chip, improve the efficiency of cell capture；The detachable use of temperature control system of this microfluidic system and chip accepter, both are connected by draw-in groove.
This utility model has the following advantages and effect: microfluidic system, temperature control system, nano chips are used in combination, the separation of rare cell and qualification in human peripheral, available high-purity and the rare cell of tool activity, be applied to downstream protein characterize, gene analysis and cell detection, joint detection results have disease diagnosis, prevent, the clinical assistant diagnosis effect such as monitoring.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present utility model.
In figure: main frame 1, Reagent Tube 11, reagent base 12, valve 13, Miniature injection pump 14, sample room 15, chip 16, chip accepter 17, waste liquid cylinder 18, computer 2, data wire 3, pipeline 4, air vent 5, exhaust fan 6, digital display screen 7, barcode scanner 8, relay indicating light 9.
Detailed description of the invention
The utility model is described in further detail below in conjunction with the accompanying drawings and by embodiment.
Seeing Fig. 1, the present embodiment includes: main frame 1, Reagent Tube 11, reagent base 12, valve 13, Miniature injection pump 14, sample room 15, chip 16, chip accepter 17, waste liquid cylinder 18, computer 2, data wire 3, pipeline 4, air vent 5, exhaust fan 6, digital display screen 7, barcode scanner 8, relay indicating light 9.
In the present embodiment, main frame 1 is provided above with reagent base 12, and reagent base 12 is placed with five Reagent Tubes 11；Being provided with eight valves 13 and Miniature injection pump 14 before main frame 1, eight valves 13 are all connected with Miniature injection pump 14, constitute eight to valve system；Chip accepter 17 and waste liquid cylinder 18 it is additionally provided with on the base of main frame 1, connect on chip accepter 17 and have sample room 15, in chip 16 is fixed on chip accepter 17 and being connected with sample room 15, the bottom surface of chip accepter 17 is with thermal control plate, and sample room 15 is internal is helical structure；Chip receiver 17 uses pipeline 4 to be connected with waste liquid cylinder 18；Having five to use pipeline 4 to be connected with five Reagent Tubes 11 respectively in eight valves 13, remaining valve 13 uses pipeline 4 to be connected with sample room 15, waste liquid cylinder 18 and air vent 5 respectively, the excessive gas that air vent 5 produces in removal system；Main frame 1 uses data wire 3 to be connected with computer 2.
In the present embodiment, main frame 1 is additionally provided with exhaust fan 6, digital display screen 7, barcode scanner 8 and relay indicating light 9.Exhaust fan 6 operating is adapted to assist in main frame 1 and dispels the heat, and digital display screen 7 can displays temperature and voltage, it is simple to the temperature of course of reaction and the control of flow rate of liquid；Barcode scanner 8 can scan various bar code, facilitates late detection result computer data to arrange；Relay indicating light 9 lights and may indicate that opening of device, extinguishes then equipment and closes.
Work process of the present utility model is as follows: power-on, relay indicating light 9 lights, uncovered chip 16 is put in chip accepter 17, thereto three Reagent Tubes 11 are separately added into buffer, purified water and pipeline flush liquid, three forms cleaning systems liquid, first by pipeline flush liquid before using, purified water, the order of buffer cleans whole system, liquid enters the blank chip 16 in sample room 15 to chip accepter 17 from Reagent Tube 11 by pipeline 4 under the effect of Miniature injection pump 14 and the control of valve 13, waste liquid cylinder 18 is entered finally by pipeline 4, cleaning completes；By barcode scanner 8 scanned samples bar code, it is separately added into cell cleanout fluid and cell fixative in remaining two Reagent Tubes 11, in sample room 15, add sample to be tested, the blank chip 16 in chip accepter 17 is replaced to the nano chips 16 being coated, then sample room 15 is connected with chip 16；Under the effect of Miniature injection pump 14 and the control of valve 13, cell cleanout fluid in one of them Reagent Tube 11 enters sample room 15 to chip 16, sample to be tested is driven to enter chip 16 and react with chip 16, residual waste solution enters waste liquid cylinder 18 by pipeline 4, carrying out several circulations, cell capture completes；Then, under the effect of Miniature injection pump 14 and the control of valve 13, the cell fixative in another Reagent Tube 11 enters sample room 15 to chip 16, waste liquid cylinder 18 is entered finally by pipeline 4, carrying out several circulations, cell has been fixed, and takes out chip 16 for follow-up further process and detection；Whole system is cleaned by the order of buffer, purified water, pipeline flush liquid after using, liquid enters sample room 15 to chip 16 from Reagent Tube 11 by pipeline 4 under the effect of Miniature injection pump 14 and the control of valve 13, waste liquid cylinder 18 is entered finally by pipeline 4, finally make pipeline flush liquid be full of whole system, prevent from polluting；Close power supply.
The part being not described in this specification all uses structure known in those skilled in the art and principle, is prior art.
By foregoing description, those skilled in the art can implement.
Furthermore, it is necessary to illustrate, the specific embodiment described in this specification, as long as its parts do not clearly state concrete specification, then these parts can be any specification adapted with its function；Meanwhile, the title that parts are taken can also be different.All equivalences done according to structure, feature and the principle described in this utility model design or simple change, be all included in the protection domain of this utility model patent.
Claims (8)
1. a circulation rare cell sorter, including main frame and computer, described main frame and computer are connected by data wire, it is characterised in that: described main frame is provided above with reagent base, and reagent base is placed with Reagent Tube；Being provided with valve and Miniature injection pump before described main frame, described valve is connected with Miniature injection pump；At least four, described valve, one of them valve connects air vent, and remaining valve is connected with Reagent Tube, sample room and waste liquid cylinder by pipeline respectively；Chip accepter and waste liquid cylinder it is provided with on described main machine base, connect on described chip accepter and have sample room, being placed with chip in described chip accepter, described chip is connected with sample room, and described chip receiver uses pipeline to be connected with waste liquid cylinder.
Circulation rare cell sorter the most according to claim 1, it is characterised in that: described valve has eight, and is all connected with same Miniature injection pump；Described Reagent Tube has five, and described five in eight valves are connected by pipeline with five described Reagent Tubes respectively.
Circulation rare cell sorter the most according to claim 1, it is characterised in that: described chip accepter is with temperature control system.
Circulation rare cell sorter the most according to claim 1, it is characterised in that: it is stamped bacteriological filtration lid on described Reagent Tube.
Circulation rare cell sorter the most according to claim 1, it is characterised in that: it is provided with exhaust fan on described main frame.
6. according to the circulation rare cell sorter described in claim 1 or 3, it is characterised in that: it is provided with digital display screen on described main frame.
Circulation rare cell sorter the most according to claim 1, it is characterised in that: it is provided with barcode scanner on described main frame.
Circulation rare cell sorter the most according to claim 1, it is characterised in that: it is provided with relay indicating light on described main frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620205301.2U CN205420426U (en) | 2016-03-17 | 2016-03-17 | Rare cell sorter circulates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620205301.2U CN205420426U (en) | 2016-03-17 | 2016-03-17 | Rare cell sorter circulates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205420426U true CN205420426U (en) | 2016-08-03 |
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ID=56547304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620205301.2U Withdrawn - After Issue CN205420426U (en) | 2016-03-17 | 2016-03-17 | Rare cell sorter circulates |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205420426U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106939281A (en) * | 2017-03-14 | 2017-07-11 | 复旦大学附属中山医院 | Circulating tumor cell sorter and its kit |
| CN107167603A (en) * | 2016-11-07 | 2017-09-15 | 杭州华得森生物技术有限公司 | Circulating tumor cell assay instrument |
-
2016
- 2016-03-17 CN CN201620205301.2U patent/CN205420426U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167603A (en) * | 2016-11-07 | 2017-09-15 | 杭州华得森生物技术有限公司 | Circulating tumor cell assay instrument |
| CN107167603B (en) * | 2016-11-07 | 2019-09-10 | 杭州华得森生物技术有限公司 | Circulating tumor cell assay instrument |
| CN106939281A (en) * | 2017-03-14 | 2017-07-11 | 复旦大学附属中山医院 | Circulating tumor cell sorter and its kit |
| CN106939281B (en) * | 2017-03-14 | 2019-09-27 | 复旦大学附属中山医院 | Circulating tumor cell sorter and its kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20160803 Effective date of abandoning: 20160823 |
|
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |