CN220019608U - Disposable device for pig, cattle and sheep brucella test tube agglutination test - Google Patents
Disposable device for pig, cattle and sheep brucella test tube agglutination test Download PDFInfo
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- CN220019608U CN220019608U CN202321572087.0U CN202321572087U CN220019608U CN 220019608 U CN220019608 U CN 220019608U CN 202321572087 U CN202321572087 U CN 202321572087U CN 220019608 U CN220019608 U CN 220019608U
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- 238000012360 testing method Methods 0.000 title claims abstract description 51
- 230000004520 agglutination Effects 0.000 title claims abstract description 27
- 241000589562 Brucella Species 0.000 title claims abstract description 18
- 241000283690 Bos taurus Species 0.000 title claims abstract description 16
- 241001494479 Pecora Species 0.000 title claims abstract description 15
- 241000282898 Sus scrofa Species 0.000 title claims description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 96
- 239000004793 Polystyrene Substances 0.000 claims abstract description 12
- 229920002223 polystyrene Polymers 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 7
- 238000002834 transmittance Methods 0.000 claims abstract description 5
- 238000001746 injection moulding Methods 0.000 claims description 6
- 241001148111 Brucella suis Species 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 10
- 241000282887 Suidae Species 0.000 abstract description 8
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- 239000004033 plastic Substances 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 206010006500 Brucellosis Diseases 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
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- 238000010586 diagram Methods 0.000 description 4
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- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000012631 diagnostic technique Methods 0.000 description 2
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- 238000000105 evaporative light scattering detection Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000002906 medical waste Substances 0.000 description 2
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- 241000894006 Bacteria Species 0.000 description 1
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- 208000035472 Zoonoses Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000000405 serological effect Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
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- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
- 206010048282 zoonosis Diseases 0.000 description 1
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Abstract
A disposable device for a Brucella test tube agglutination test of pigs, cattle and sheep. The eight-joint reaction tube is made of high-light-transmittance polystyrene materials, the whole eight-joint reaction tube is in a strip shape, and one end of the eight-joint reaction tube is provided with a handheld part which is convenient to hold and has a flat protruding structure; the connecting pipe cover is formed with eight downwardly protruding covers which are matched with eight reaction pipes of the eight-connecting reaction pipes. The disposable device adopts the eight-joint reaction tube and the joint tube cover, and adopts high-light-transmission polystyrene plastic, so that an experimenter can conveniently pick up uniformly-mixed reaction reagents and transparent observation results when detecting in batches, liquid in the tube cannot splash out, and meanwhile, the reagent is prevented from volatilizing in the experimental process.
Description
Technical Field
The utility model relates to the technical field of prevention, control and detection of animal epidemic diseases, in particular to a disposable device for a test tube agglutination test of Brucella in pigs, cattle and sheep.
Background
Brucellosis, also known as brucellosis, is caused mainly by brucellosis infection, and is a worldwide and widely prevalent zoonotic disease. Slaughter personnel and breeders who have a history of intimate contact with cattle, sheep and pigs are high risk groups for developing disease. If the patient is not treated timely and effectively, the patient can be changed from acute to chronic, and the hidden focus is induced, so that the patient's illness is repeatedly started, and the physical health and the normal work ability of the patient are seriously affected. Early diagnosis is of great significance in reasonably preparing treatment schemes and improving prognosis of patients with cloth disease. Although the traditional bacteria culture detection method has higher accuracy, the biological safety risk is high, the infection is easy to spread, the time consumption is longer, the cost is high, and operators need to have professional technical quality. Along with the development of the inspection and analysis technology, the serological agglutination test of the brucellosis becomes an effective detection method in clinic at present, and has the advantages of convenient and rapid operation, easy popularization and convenient judgment. Among them, the tube agglutination test is the most widely used, most effective representative primary screening test method.
Although the test tube agglutination test is taken as an authoritative and effective confirmation method, the test tube agglutination test still has some problems and natural defects, such as the test material sampling 96-hole U-shaped polystyrene plate in the method (4.6.1 micro-method) of test tube agglutination test (SAT) of the current national standard GB/T18646-2018 animal brucellosis diagnosis technology, and the maximum capacity of each hole of the 96-hole polystyrene plate in the market is only 350 to 400ul, so that the test result is inconvenient to observe remarkably due to too little solution quantity; in addition, the 96-well plate has the problems of small space and high density, and difficult observation result after the reaction is finished. So most laboratories do not basically use the 4.6.1 micro method when performing experimental operations, but use the 4.6.2 constant method to select Conn cuvette (glass cuvette) instead of experiments. However, the use of the Conse cuvette also has the following problems:
1. for the experimental operator, a marker is needed to mark the sample information on each Kangshi tube;
2. the Kangshi tube is made of glass, the tube wall is very thin, the Kangshi tube is easy to damage due to carelessness in operation, and the hand is easy to cut;
3. when the test tube agglutination experimental method-constant method detection of the national standard GB/T18646-2018 animal brucellosis diagnostic technique is adopted, as each sample needs to occupy 4 hole sites after dilution by multiple ratio, when a large amount of samples are intensively detected, a plurality of Conn's colorimetric tubes are inconvenient for an experimenter to take up and mix the reaction reagent and the observation result, and are inconvenient to place and fix; in addition, the Cone colorimetric tube is of an open design, and the reagent is easy to volatilize due to overnight reaction in a 37 ℃ incubator, so that most of experimenters can only seal the Cone colorimetric tube one by using a self-sealing film, and the exposure risk of pathogenic microorganisms is caused;
4. most laboratories can clean, dry and disinfect the Confucian colorimetric tubes after the experiment is finished, and recover the Confucian colorimetric tubes, so that the experiment time is prolonged intangibly, and the burden of an experimenter is increased;
5. repeated use of the Cone cuvette may cause residual Brucella positive samples to cause infection to the laboratory staff, especially under insufficient sterilization.
There is also experimental apparatus that adopts many test tube samplings among the prior art, like patent No. "202120335547.2", a nucleic acid extraction bar magnet cover and extraction element "a nucleic acid extraction bar magnet cover that discloses, it includes base plate and strip sleeve pipe subassembly, strip sleeve pipe subassembly includes a plurality of connecting plates and a plurality of sleeve pipes, sheathed tube top and the lower fixed surface of connecting plate are connected, the through-hole has been seted up on the connecting plate, through-hole and sleeve pipe intercommunication, a plurality of connecting plates are arranged in proper order, the base plate is located the one end of connecting plate arrangement direction, interconnect between the adjacent connecting plate, base plate and adjacent connecting plate interconnect, be fixed with the strengthening rib on the lower surface of connecting plate. The device of this patent has several drawbacks and disadvantages: 1. because the upper end of the sleeve pipe component of the magnetic rod sleeve is designed to be an opening, a pipe cover corresponding to each other is not designed, and when an experimenter picks up a uniformly mixed reaction reagent and observes the result, liquid in the pipe is easy to splash out; 2. the magnetic rod sleeve is made of white materials, can not be observed through, and can not be observed basically for weak positive samples; 3. the device is mainly used for the purpose of extracting and purifying sample nucleic acid, and is not suitable for the operation of a brucella tube agglutination test.
Disclosure of Invention
The utility model aims to solve the technical problems and provide a disposable device for a pig, cattle and sheep brucella test tube agglutination test, which adopts an eight-linked reaction tube and a connecting tube cover, and adopts high-light-transmission polystyrene plastic, so that an experimenter can conveniently pick up uniformly mixed reaction reagents and transparent observation results during mass detection, liquid in the tube cannot splash, and meanwhile, the eight-linked reaction tube and the tube cover are disposable medical wastes, so that secondary infection risks are avoided.
The technical scheme of the utility model is as follows:
a disposable device for a pig, cattle and sheep Brucella test tube agglutination test comprises a plurality of eight-linked reaction tubes, a plurality of connecting tube covers matched with the eight-linked reaction tubes, and deep hole plates for inserting and fixing the eight-linked reaction tubes, wherein the eight-linked reaction tubes are integrally strip-shaped and comprise eight reaction tubes which are arranged in parallel, the eight-linked reaction tubes are made of high-light-transmittance polystyrene materials, the eight-linked reaction tubes are integrally strip-shaped, and one end of each eight-linked reaction tube is provided with a handheld part which is convenient to hold and has a flat protruding structure;
the connecting pipe cover is formed with eight downwardly protruding covers, the eight downwardly protruding covers are matched with eight reaction pipes of the eight-connecting reaction pipes, and the eight covers can be pressed into pipe orifices of the eight reaction pipes respectively for preventing leakage and evaporation of reagents.
Preferably, the eight-connected reaction tubes are integrally formed by injection molding, the tube body of each reaction tube is round, the bottom of the tube body is flat bottom, and each reaction tube is of a blind hole structure with an opening at the top;
further, one end of the single eight-linked reaction tube, which is close to the hand-held part, is connected with a single connecting tube cover, and the connecting tube cover can downwards cover the eight-linked reaction tube and can upwards open the eight-linked reaction tube;
further, the deep hole plate is a 96 hole plate, the deep hole plate is made of polystyrene by injection molding, 96 square deep holes are uniformly formed in the upward direction of the deep hole plate, the number of the 96 square deep holes is 12 in the transverse direction and 8 in the vertical direction, the deep hole plate is matched with eight reaction tubes, the eight reaction tubes can be inserted and fixed into the deep hole plate, the eight reaction tubes of the eight reaction tubes can be respectively inserted into the square deep holes of the deep hole plate, the eight reaction tubes are conveniently fixed in the experimental process and do not shake, meanwhile, the condition of a reagent reaction interface is conveniently observed, and compared with the traditional test tube rack, the test tube rack is very light and convenient, and does not occupy space;
further, the brucella test tube agglutination test is further provided with a box structure, the box structure is provided with a fixing frame for suspending the eight-joint reaction tube, when the eight-joint reaction tube is placed in the box structure, the front end and the rear end of the fixing frame respectively clamp the two ends of the eight-joint reaction tube, the box structure is further provided with a lamplight device, the reaction tube of the eight-joint reaction tube can be suspended and fixedly watched against lamplight, and the observation result is more convenient.
The utility model discloses a disposable device for a brucella tube agglutination test of pigs, cattle and sheep, which has the beneficial effects that:
1. eight reaction tubes are adopted, and a matched connecting tube cover is arranged, so that eight reaction tubes can be operated at one time during mass detection, an experimenter can conveniently pick up uniformly mixed reaction reagents and observation results, and liquid in the tubes cannot splash. When the test tube agglutination experimental method-constant method detection of the national standard GB/T18646-2018 animal brucellosis diagnostic technique is adopted, the reaction lattice of each octave tube design is 8, and each sample needs to occupy 4 hole sites after dilution by double ratio, so that a single octave tube can be used for detecting 2 samples, and 24 samples can be made at most on a deep pore plate at one time, thus greatly improving the experimental efficiency, saving time and labor;
2. the reaction tube material adopts high light transmittance polystyrene plastic, can be conveniently picked up for transparent observation of light, is very clear in naked eye judgment even a weak positive sample, and is not easy to damage;
3. the eight-joint reaction tube and the tube cover are disposable medical waste, and can be sterilized under high pressure without uncovering after use, so that the risk of infection is avoided;
4. the 96-hole deep hole plate can be reused after being sterilized, and no waste exists.
Drawings
FIG. 1 is a block diagram of an octant reaction tube of a disposable device for a Brucella test tube agglutination test of pigs, cattle and sheep according to the present utility model;
FIG. 2 is a schematic diagram showing the connection of the eight-linked reaction tube and the connecting tube cover of the disposable device for the test tube agglutination test of Brucella suis, cattle and sheep;
FIG. 3 is a block diagram of a deep well plate of a disposable device for a Brucella test tube agglutination test of pigs, cattle and sheep according to the present utility model;
FIG. 4 is a block diagram showing the structure of a disposable device for the test tube agglutination test of Brucella suis in pigs, cattle and sheep according to the present utility model.
Detailed Description
The utility model is further illustrated by the following examples:
examples:
referring to fig. 1 to 4, a disposable device for a test tube agglutination test of brucella in pigs, cattle and sheep comprises a plurality of eight-linked reaction tubes 1, a plurality of connecting tube covers 2 matched with the eight-linked reaction tubes, and deep hole plates 3 for inserting and fixing the eight-linked reaction tubes, wherein the eight-linked reaction tubes are integrally strip-shaped and comprise eight reaction tubes 11 arranged in parallel, the eight-linked reaction tubes are made of high-light-transmittance polystyrene materials, the eight-linked reaction tubes are integrally strip-shaped, and one end of each eight-linked reaction tube is provided with a handheld part 12 which is convenient to hold and is of a flat protruding structure;
the coupling tube cover 2 is formed with eight downwardly protruding covers 21, which are matched with eight reaction tubes of the eight coupling reaction tubes, and the eight covers can be respectively pressed into the mouths of the eight reaction tubes for preventing leakage and evaporation of the reagent.
Preferably, the eight-connected reaction tubes are integrally formed by injection molding, the tube body of each reaction tube is round, the bottom of the tube body is flat bottom, and each reaction tube is of a blind hole structure with an opening at the top; the volume of the single reaction tube is 1000ul and marked with scales; the experimental requirements are met;
further, one end of the single eight-linked reaction tube, which is close to the hand-held part, is connected with a single connecting tube cover, and the connecting tube cover can downwards cover the eight-linked reaction tube and can upwards open the eight-linked reaction tube;
further, the deep hole plate 3 is a 96-hole plate, the deep hole plate is made of polystyrene by injection molding, 96 square deep holes 31 are uniformly formed in the upward direction of the deep hole plate, the number of the 96 square deep holes is 12 in the transverse direction and 8 in the vertical direction, the deep hole plate is matched with eight-joint reaction tubes, the eight-joint reaction tubes can be inserted and fixed into the deep hole plate, the eight reaction tubes of the eight-joint reaction tubes can be respectively inserted into the square deep holes of the deep hole plate, the eight-joint reaction tubes are conveniently fixed without shaking in the experimental process, and meanwhile, the condition of a reagent reaction interface is conveniently observed;
further, the brucella test tube agglutination test is also provided with a box structure 4, the box structure is provided with a fixing frame 41 for suspending the eight-joint reaction tube, when the eight-joint reaction tube is placed in the box structure, the front end and the rear end of the fixing frame respectively clamp the two ends of the eight-joint reaction tube, the box structure is also provided with a lamplight device, the reaction tube of the eight-joint reaction tube can be suspended and fixedly watched against lamplight, and the observation result is more convenient.
The foregoing detailed description of the utility model is merely illustrative of preferred embodiments of the utility model, and it is intended that the utility model not be limited to the details of the preferred embodiments of the utility model, but that the utility model is capable of modification and variation in accordance with the general principles of the utility model.
Claims (4)
1. A disposable device for pig cattle sheep brucella test tube agglutination test, its characterized in that:
the eight-joint reaction tube is made of high-light-transmittance polystyrene materials, the whole eight-joint reaction tube is in a strip shape, and one end of the eight-joint reaction tube is provided with a handheld part which is convenient to hold and has a flat protruding structure;
the connecting pipe cover is provided with eight downwards-protruding cover bodies which are matched with eight reaction pipes of the eight-connecting reaction pipes, and the eight cover bodies can be respectively pressed into pipe orifices of the eight reaction pipes;
one end of the single eight-linked reaction tube, which is close to the hand-held part, is connected with a single connecting tube cover, and the connecting tube cover can downwards cover the eight-linked reaction tube and can upwards open.
2. The disposable device for the agglutination test of the brucella suis test tube according to claim 1, wherein the eight-joint reaction tube is integrally formed by injection molding, the tube body of the single reaction tube is round, the bottom of the tube body is flat bottom, and the single reaction tube is of a blind hole structure with an open top.
3. The disposable device for the pig, cattle and sheep brucella test tube agglutination test according to claim 1, wherein the deep hole plate is a 96-hole plate, the deep hole plate is made of polystyrene by injection molding, 96 square deep holes are uniformly formed in the upward direction of the deep hole plate, the number of the 96 square deep holes is 12 in the transverse direction and 8 in the vertical direction, the deep hole plate is matched with the eight-connection reaction tubes, the eight-connection reaction tubes can be inserted and fixed into the deep hole plate, and the eight reaction tubes of the eight-connection reaction tubes can be respectively inserted into the square deep holes of the deep hole plate.
4. The disposable device for the pig, cow and sheep brucella tube agglutination test according to claim 1, wherein the brucella tube agglutination test is further provided with a box structure, the box structure is provided with a fixing frame for suspending the eight-linked reaction tube, when the eight-linked reaction tube is placed in the box structure, the front end and the rear end of the fixing frame respectively clamp the two ends of the eight-linked reaction tube, and the box structure is further provided with a light device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321572087.0U CN220019608U (en) | 2023-06-20 | 2023-06-20 | Disposable device for pig, cattle and sheep brucella test tube agglutination test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321572087.0U CN220019608U (en) | 2023-06-20 | 2023-06-20 | Disposable device for pig, cattle and sheep brucella test tube agglutination test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220019608U true CN220019608U (en) | 2023-11-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321572087.0U Active CN220019608U (en) | 2023-06-20 | 2023-06-20 | Disposable device for pig, cattle and sheep brucella test tube agglutination test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220019608U (en) |
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2023
- 2023-06-20 CN CN202321572087.0U patent/CN220019608U/en active Active
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