CN108226547A - Circulating tumor cell detecting instrument including micro-fluidic chip - Google Patents
Circulating tumor cell detecting instrument including micro-fluidic chip Download PDFInfo
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- CN108226547A CN108226547A CN201711414340.9A CN201711414340A CN108226547A CN 108226547 A CN108226547 A CN 108226547A CN 201711414340 A CN201711414340 A CN 201711414340A CN 108226547 A CN108226547 A CN 108226547A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
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Abstract
The invention discloses a kind of circulating tumor cell detecting instruments including micro-fluidic chip, further include sampling system, fluid collection device, signal processing and analysis system, core controller and display control interface, core controller and sampling system, signal processing and analysis system and display control interface are electrically connected to control running, sampling system is connect with micro-fluidic chip sample inlet according to core controller order-driven Sample introduction chip, fluid collection device is connect with micro-fluidic chip sample export collects enriched obtained target liquid and waste liquid, signal processing and analysis system analyzes the response electric signal of detecting electrode region impedance variations induction according to core controller instruction acquisition, display control interface instructs display data result according to core controller.The present invention does not need to label cell, and processing flux is high, is automatically separated the live body circulating tumor cell for obtaining high-purity, test process is simple, efficient, applied widely.
Description
Technical field
The present invention relates to cell enrichment instruments, and in particular to a kind of circulating tumor cell detector including micro-fluidic chip
Device.
Background technology
Modern medicine study shows
Cause " seed " of cancer metastasis.Therefore, circulating tumor cell has important clinical diagnosis and scientific research value, main body
Now in：(1) detect circulating tumor cell in blood has higher precision than the conventional diagnostics such as radiography technology, can be the morning of cancer
Phase diagnoses and prognosis evaluation provides new method means, is referred to as liquid Biopsy.(2) with the heredity identical with primary tumor
Information can provide important sample for the research of cancer metastasis mechanism and the screening of anticancer drug.But due to circulating tumor in blood
The quantity of cell is extremely rare, this proposes stern challenge to efficiently separating and detecting rare circulating tumor cell.
Circulating tumor cell detecting instrument CellSearch systems currently on the market, the instrument pass through immunocapture and glimmering
Light enumerates the number to count rare circulating tumor cell in 7.5 milliliters of cancer plasmas, and the existence of patient is predicted with this
Phase.Other than the equipment, more and more circulating tumor cell detection prototype machines are studied report, such as the magnetic of Cynvenio
Mark detecting system, the CTC-iChip platforms reversely marked based on leucocyte etc..But above-mentioned instrument is mainly by complex procedures
Immune labeled method, there is (1) needs by expensive immune biochemical reagent, and testing cost height, marking procedures greatly improved
The processing flux of unit interval is limited simultaneously；(2) high specificity so that instrument is only applicable to specific several expression specificity marks
The cancer disease of will object such as epithelium adhesion factor；(3) loss of activity is not used to subsequent quantitation biology by cell after labeled
Learn research.
Invention content
Goal of the invention：The present invention provides a kind of circulating tumor cell detecting instrument including micro-fluidic chip, solves existing
There is instrument to need to mark cell, processing flux is small, and testing cost is high, is applicable in the problem of disease is few.
Technical solution：A kind of micro-fluidic chip of the present invention, including stack from top to down inertia sorting module and
Electrical impedance detection module, the inertia sort and several fluid channel separating structures are provided in module, and the electrical impedance detects mould
The sinusoidal prefocus inertia runner of series connection and microelectrode sensing runner are provided on block, is connected on the microelectrode sensing runner
Microelectrode is detected, positioning round orifice is both provided on the inertia sorting module and the corresponding position in electrical impedance detection module periphery.
In order to carry out stability of flow and adjusting, the inertia sorting to the sample flow of micro-fluidic chip and other auxiliary fluids
Module stacked on top has flow-rate adjustment stable module, sets multiple passive flux regulating valves and flow manifold thereon, makes sample flow
Divided equally after stabilization by flow manifold and exported.
In order to improve the processing flux of unit interval, several fluid channel separating structures in parallel or series, divide by fluid channel
It is helical flow path structure to select structure.
In order to which difference reduces the signal of acellular blank background liquid generation, microelectrode is detected as a pair or two pairs, and
It is each pair of to be symmetrically distributed in the microelectrode sensing runner left and right sides or upper and lower both sides.
Each module alignment is installed for convenience, the flow-rate adjustment stable module, inertia sorting module and electrical impedance detection
Positioning round orifice is both provided on the corresponding position in module periphery, positioning round orifice quantity is four.
Circulating tumor cell detecting instrument of the present invention including micro-fluidic chip, further includes sampling system, liquid
Collection device, signal processing and analysis system, core controller and display control interface, core controller and sampling system, signal
Acquisition analysis system and display control interface are electrically connected to control running, the sampling system and the micro-fluidic chip sample
Entrance connection is according to core controller order-driven Sample introduction chip, the fluid collection device and the micro-fluidic chip sample
Enriched obtained target liquid and waste liquid is collected in product outlet connection, and the signal processing and analysis system connect root with detection microelectrode
According to the response electric signal that core controller instruction acquisition analysis detecting electrode region impedance variations induce, the display control
Interface instructs display data result according to core controller.
In order to sample and buffer solution be pushed to import micro-fluidic chip by air pressure, the sampling system includes coupon, delays
Fliud flushing bottle and diaphragm pump, the developmental tube and buffer solution bottle are connect by airtight pipeline with diaphragm pump, and pass through fluid path silicone tube
It is connect with micro-fluidic chip sample inlet, diaphragm pump, which generates driving air source, drives the liquid in the coupon and buffer solution bottle to be led
Enter micro-fluidic chip.
For the break-make according to pressure control gas circuit, the sampling system further includes solenoid valve and pressure sensor, electromagnetism
Valve is arranged on the break-make that GT tube road controls gas circuit, and solenoid valve and diaphragm pump are electrically connected and with pressure sensor according to pressure
The pressure information control running of sensor feedback.
In order to control the break-make of fluid path, the logical of electromagnet disabling mechanism control fluid path is provided on the fluid path silicone tube
It is disconnected.
Micro-fluidic chip is replaced in order to facilitate dismounting Ei, the micro-fluidic chip is removably installed by slot
On circulating tumor cell detecting instrument.
Advantageous effect：The present invention does not need to label cell, and processing flux is high, is automatically separated the live body cycle for obtaining high-purity
Tumour cell, and the detectable multiple biophysical parameters for obtaining cell, test process is simple, efficient, applied widely.
Description of the drawings
Fig. 1 is circulating tumor cell detecting instrument overall structure diagram；
Fig. 2 is internal structure schematic diagram after instrument removal shell and partition board；
Fig. 3 is the explosive view of micro-fluidic chip；
Fig. 4 is the detail plan structure chart of flow-rate adjustment stable module；
Fig. 5 is the planar structure schematic diagram of inertia sorting module；
Fig. 6 is the planar structure schematic diagram of electrical impedance detection module；
Fig. 7 is the structure diagram of gas circuit control unit；
Fig. 8 is the structure diagram of fluid path control unit；
Fig. 9 is the operational process schematic diagram of signal processing and analysis system；
Figure 10 is air pressure and the output characteristics figure of sheath flow quantity and sample flow；
The sampling that Figure 11 is the initial sample of blood for adulterating cancer cell, collects samples through the enrichment of instrument 1 time and 2 enrichments is micro-
Mirror photo；
Figure 12 is that the statistics haemocyte removal rate obtained after experiment and cancer cell rate of recovery data are repeated several times；
Figure 13 is the focusing effect statistical distribution figure of different sized particles in prefocus sine runner；
Figure 14 is the electrical impedance spectrogram that different sized particles detect acquisition under DC mode；
Figure 15 is to adulterate electrical impedance spectrum and the analysis statistics scatter plot that cancer cell blood obtains after detection zone.
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings.
As shown in Figs. 1-2, circulating tumor cell detecting instrument, including micro-fluidic chip 1, sampling system 2, liquid collect dress
It puts, signal processing and analysis system 3, core controller 4 and display control interface 5.Core controller 4 and sampling system 2, signal is adopted
Set analysis system 3 and display control interface 5 are electrically connected to control running, and sampling system 2 connects with 1 sample inlet of micro-fluidic chip
It connects according to 4 order-driven Sample introduction chip of core controller, fluid collection device connect receipts with 1 sample export of micro-fluidic chip
Collect enriched obtained target liquid and waste liquid, fluid collection device includes target sample pipe 224 and waste liquid bottle 226, signal acquisition
Analysis system 3 connect with detection microelectrode 133 and analyzes detecting electrode region impedance according to 4 instruction acquisition of core controller
Change the response electric signal induced, display control interface 5 instructs display data result according to core controller 4.
As shown in figure 3, micro-fluidic chip 1 is the core cell of instrument, the flow-rate adjustment stable module stacked from top to down
11st, inertia sorting module 12 and electrical impedance detection module 13, micro-fluidic chip 1 are removably installed by slot 227
On circulating tumor cell detecting instrument, micro-fluidic chip 1 can be integrated, and plastics, polymer thin can be used in material
One kind in film, rubber, silicon-based semiconductor material, processing method can be micro injection molding, laser combination thermoplastic envelope, fine machine
The manufacturing process such as processing and accurate 3D printing.Micro-fluidic chip 1 is assemblied in fixture 14, and instrument is directly inserted in by dedicated slot
On device, the injection and extraction of fluid are realized.Micro-fluidic chip 1 is set as disposable, jettisonable uses, dirty to avoid intersecting
Dye.Sample flow adjust stable module 11 be nonessential module, be mainly used for respectively to inject micro-fluidic chip 1 sample flow and
Other auxiliary fluids, assist fluid such as blank sheath fluid or cleaning solution, carry out stability of flow and adjusting.Its alternative construction such as Fig. 4 institutes
Show, multiple passive flux regulating valves are arranged in module, and a passive flux regulating valve 111 is arranged for stablizing in specific central area
The flow of sample flow is injected, and passes through flow manifold 113 and divides equally output.8 identical passive flux regulating valves 112 of arrangement up and down
For stablizing the flow of blank sheath fluid.The Flow Output Property of monomer passive flux regulating valve can be by changing parameter of structure design
Realize regulation and control, the overall output flow of flow-rate adjustment stable module 11 can be by changing parallel monomer passive flux regulating valve
111st, 112 quantity obtain.Sample flow adjusts arrangement positioning round orifice 114 realization of 11 periphery of stable module and the alignment of other modules
Assembling.As shown in figure 5, the cell high throughput inertia sorts module 12 by multiple fluid channel separating structures 121 in parallel or series
It forms, to improve the processing flux of unit interval, fluid channel separating structure 121 uses helical flow path structure, other also can be used
Inertia sorts flow passage structure, according to the size difference between circulating tumor cell and haemocyte, using in inertia micro-channel structure
The microfluid inertia effect generated (inertia migrates and Dean Secondary Flows) is induced so that different size cells focus on balance to runner
The different location of width direction, finally from different outlet separation export.The module design is avoided using immune labeled method,
The non-marked for realizing circulating tumor cell and haemocyte using the size difference of pure physics sorts, and can effectively collect acquisition live body and follow
Ring tumour cell sample.The monomer helical flow path of serial or parallel connection can be used in the processing flux of the cell inertia sorting module 12
Quantity controls.12 periphery of cell high throughput inertia sorting module arrangement positioning round orifice 122 realizes that the alignment with other modules fills
Match.
As shown in fig. 6, the electrical impedance detection module 13 senses runner by sinusoidal prefocus inertia runner 131 and microelectrode
132 are connected in series, it is sorted after circulating tumor cell be completely refocused and be arranged in sinusoidal prefocus inertia runner 131
Regular particles arrange, and one by one by detection zone, and microelectrode sensing runner 132 detects microelectrode 133 as a pair or two pairs, detection
Pattern is direct current or exchange, in the case of two pairs of microelectrodes 133, can difference subduction background (acellular blank background liquid) generate
Signal.What is obtained during low frequency under AC mode is the dimension information of cell, what when high frequency, obtained be the dielectric parameter of cell such as
Cell membrane capacitance, cytoplasm resistance etc., and the number of cell is obtained by the electrical impedance peak number amount generated.It is obtained under DC mode
Be cell size and quantity information.Sinusoidal prefocus inertia runner 131 can be that symmetrically or non-symmetrically form, sine wave can be
Circle wave or square wave.Microelectrode 133 can be that silver/silver chloride electrode is formed with high concentration electric medium in microelectrode sensing runner 132
Liquid electrode also can be metal electrode, indium and tin oxide film electrode.Positioning round orifice is arranged on 13 periphery of electrical impedance detection module
134 realize that the alignment with other modules is assembled.
As Figure 7-8, sampling system includes coupon 223, buffer solution bottle 225, diaphragm pump 211, solenoid valve 212 and pressure
Force snesor 213, sampling system 2 are controlled by gas circuit control unit 21 and fluid path control unit 22, wherein, gas circuit control unit by
Diaphragm pump 211, solenoid valve 212, pressure sensor 213 and air-tightness pipeline 214 are formed, and diaphragm pump 211 drives gas for generating
Source；Solenoid valve 212 is used to control the break-make of gas circuit；Pressure sensor 213 is used to monitor air pressure in pipeline, and feedback control diaphragm
The running of pump 211 and solenoid valve 212, developmental tube 223 and buffer solution bottle 225 are connect by airtight pipeline 214 with diaphragm pump 211,
And pass through fluid path silicone tube 222 and connect with 1 sample inlet of micro-fluidic chip, diaphragm pump 211 generates driving air source and drives coupon
223 and buffer solution bottle 225 in liquid import micro-fluidic chip 1.Solenoid valve 212 is arranged on airtight pipeline 214 and controls gas circuit
Break-make, the gas that solenoid valve 212 and diaphragm pump 211 are electrically connected with pressure sensor 213 and are fed back according to pressure sensor 213
Press information control running.Fluid path control unit 22 has electromagnet disabling mechanism 221 and silicone tube 222 to form, and is provided by gas circuit
Air pressure the liquid of sample cell 224 and buffer solution bottle 225 is pushed to enter silicone tube 222, and the break-make of fluid path adds by electromagnet
Disabling mechanism 221 is pushed to realize after electricity.Live body circulating tumor cell after enriched and detection flows into target sample pipe 224 and receives
Collection, the haemocyte waste liquid after separation are then collected into waste liquid bottle 226.Wherein, under sorting pattern, buffer solution and sample liquid are divided
Not Zhu Ru helical duct two entrances, buffer solution play the role of auxiliary sorting.In a cleaning mode, buffer solution plays cleaning
The effect of pipeline.
As shown in figure 9, signal processing and analysis system 3 includes the generation of pumping signal, the acquisition of response signal and amplification, letter
Number processing and the Core Features such as analysis.It is connect with detection microelectrode, the specific direct current of generation or ac-excited signal is passed through
Convert it is after-applied in detection microelectrode 133 on, when cell by detection microelectrode 133 when due to its space occupation time process, cell
The background solution of respective volume will be replaced, so as to cause the variation of two detection 133 impedances of microelectrode.The response telecommunications of induction
Number by conversion and amplification, and through noise reduction and extraction after be transmitted to computer or instrument integration processor software system, finally may be used
Analysis obtains cell biological physical characteristic data.
In whole device, core controller 4 controls diaphragm pump 211, solenoid valve 212, pressure sensor 213, electromagnet to cut
Locking mechanism 221 connects, the running of signal processing and analysis system 3, and it is clear to have circulating tumor cell enrichment, concentration, detection and pipeline
Multiple functions pattern is washed, sorting pattern obtains target circulation tumour cell by being detached in blood sample, removes a large amount of existing
Background blood cells；Concentration formats in the target circulation tumor cells specimens of collection for will further remove blank solution, to carry
The concentration of high circulation tumour cell；Detection pattern is that further extraction obtains live body circulating tumor cell on the basis of Enrichment Mode
A variety of biophysical properties data.Display control interface 5 is used to implement the control of instrument operation, the alarm of instrument problems, work(
The output of energy pattern switching and testing result is shown.
When being detected with the detector of the present invention, self-test, the functional initialization of institute are completed in instrument booting first；In slot 227
Middle insertion is integrated can to abandon micro-fluidic chip 1, load the coupon 223 containing blood to be measured, sample collection tube 224, and filling slow
Fliud flushing is to buffer solution bottle 225.Instrument start key is pressed, also self-defined according to demand can select specific monomer functional mode therein
As sorted, concentrating, detecting and pipeline-cleaning.Instrument automatic running, separation obtain the live body circulating tumor cell sample of high-purity,
It and can a variety of biophysical information data of label-free detection acquisition.Instrument carries out pipeline-cleaning, abandon it is integrated can abandon it is micro-fluidic
Chip 1.Wherein, as shown in figure 9, signal processing and analysis system idiographic flow is that programming generates m-sequence digital signal first, through D/
A is converted to the after-applied response current priority to detecting on microelectrode 133, obtained from microelectrode 133 of analog signal by poor
It is acquired after dividing amplifier, low pass filter conversion, applying quick M to collected digital signal changes to obtain the arteries and veins of system
Punching response, and then it is that can obtain the wideband impedance information of cell to apply Fast Fourier Transform (FFT).Wherein, quick M variations are to realize
A kind of fast method of system response signal and m-sequence pumping signal correlation computations.Figure 10 be develop instrument system pressure and
Sheath fluid and sample liquid output flow performance plot.As seen from the figure, gradual increasing is presented with the work of diaphragm pump 211 in system air pressure
Add, pressure is down to zero when instrument is closed.Sample flow and sheath flow quantity store power in air pressure, and solenoid valve 212 and electromagnet are cut
After locking mechanism 221 is decontroled, instantaneous fluctuation is presented, the immediate stability therewith the presence that stable module 11 is adjusted due to sample flow
To preset value.To reduce influence of the flowed fluctuation to cell sorting and detection result, instrument running section, i.e. instrument can be carried out
Cell sorting and the time section of detection are slightly delayed.
Sample is detected using detecting instrument of the present invention, specific sample is the blood sample for adulterating cancer cell, is accurate table
Separation results are levied, more fluorescent marker breast cancer cell is mixed in 1 milliliter of blood.Once enrichment and two is carried out to sample
After secondary enrichment, with microscope photographing collect target sample microphoto it is as shown in figure 11, be above classified as brightfield mode, under be classified as fluorescence
Pattern, (a) are the sampling microphoto of initial sample liquid, and (b) is the sampling microphoto after primary enrichment, and (c) is rich twice
Sampling microphoto after collection, for shown in the statistical result Figure 12 obtained after different sample test of many times the result shows that
It was found that after completing primary enrichment, the removal rate of haemocyte has reached more than 93%, and cancer cell capture rate is about 85%.To above-mentioned
Product liquid carries out secondary enrichment, after further removing the haemocyte in product liquid, find haemocyte removal rate rise to 99% with
On, and cancer cell capture rate slightly drops to 80% or so.In order to obtain sinusoidal 131 exit region of prefocus inertia runner, runner
50 microns of width, 25 microns of runner height, 4 centimetres, 10 microns, 15 microns and 20 microns compound particles of flow channel length 50~
Focusing alignment characteristics under 200 mul/min of flows shoot Particles Moving picture, and by image stack by high-speed camera
The folded particle distribution trajectory diagram obtained later is as shown in figure 13, and as seen from the figure, good row is presented when entering detection zone for particle
Row particle row, so as to avoid multiple cell/particles simultaneously pass through detection zone caused by false error detection result.Figure 14
To detect 10 microns, 15 microns and 20 micro particles electrical impedance signal spectrums of acquisition under DC mode, single particle is by detection
Microelectrode 133 obtains a signal peak, and the quantity at statistical signal peak can accurately obtain the number by particle.Meanwhile signal peak
Peak value and particle/cell size to be detected between apparent linear corresponding relation is presented, pass through calibration and cell ruler can be achieved
Very little accurate detection.Figure 15 tops are the electrical impedance signal spectrum of doping breast cancer cell line (MCF-7) in splitting erythrocyte blood.
Lower part obtains electrical impedance signal peak value and peak width scatter plot for statistics.As seen from the figure, the peak value of electrical impedance signal peak can be passed through
Effectively differentiate leucocyte and breast cancer cell.Other a variety of biophysical properties data can also be obtained under AC mode.
Claims (10)
1. a kind of micro-fluidic chip (1), which is characterized in that including the inertia sorting module (12) stacked from top to down and electrical impedance
Detection module (13), the inertia sort and are provided with several fluid channel separating structures (121), the electrical impedance in module (12)
The sinusoidal prefocus inertia runner (131) of series connection and microelectrode sensing runner (132) are provided on detection module (13), it is described micro-
Detection microelectrode (133) is connected on electrode sensing runner (132).
2. micro-fluidic chip according to claim 1, which is characterized in that the inertia sorting module stacked on top has flow
Stable module 11 is adjusted, multiple passive flux regulating valves and flow manifold are set thereon, sample flow is made to be flowed after stablizing by shunting
Road, which is divided equally, to be exported.
3. micro-fluidic chip according to claim 1, which is characterized in that several fluid channel separating structures (121) are simultaneously
Connection or series connection, fluid channel separating structure (121) are helical flow path structure.
4. micro-fluidic chip according to claim 1, which is characterized in that the detection microelectrode (133) is a pair or two
It is right, and each pair of microelectrode that is symmetrically distributed in senses runner (132) left and right sides or upper and lower both sides.
5. micro-fluidic chip according to claim 2, which is characterized in that the flow-rate adjustment stable module (11), inertia
Sorting module (12) and electrical impedance detection module (13) periphery is both provided with positioning round orifice on corresponding position.
6. a kind of circulating tumor cell detecting instrument including micro-fluidic chip as described in claim 1, which is characterized in that also
Including sampling system (2), fluid collection device, signal processing and analysis system (3), core controller (4) and display control interface
(5), core controller (4) and sampling system (2), signal processing and analysis system (3) and display control interface (5) be electrically connected with
Control running, the sampling system (2) connect with the micro-fluidic chip (1) sample inlet to be instructed according to core controller (4)
Sample introduction chip is driven, the fluid collection device is connect with the micro-fluidic chip (1) sample export collects enriched obtain
The target liquid and waste liquid arrived, the signal processing and analysis system (3) are connect with detection microelectrode (133) according to core controller
(4) the response electric signal that instruction acquisition analysis detection microelectrode (133) region impedance variations induce, the display control interface
(5) display data result is instructed according to core controller (4).
7. circulating tumor cell detecting instrument according to claim 6, which is characterized in that the sampling system (2) includes
Coupon (223), buffer solution bottle (225) and diaphragm pump (211), the coupon (223) and buffer solution bottle (225) are by airtight
Pipeline (214) is connect with diaphragm pump (211), and is passed through fluid path silicone tube (222) and connect with 1 sample inlet of micro-fluidic chip, every
Membrane pump (211), which generates driving air source, drives the liquid in the coupon (223) and buffer solution bottle (225) to import micro-fluidic chip
1。
8. circulating tumor cell detecting instrument according to claim 7, which is characterized in that the sampling system 2 further includes
Solenoid valve (212) and pressure sensor (213), solenoid valve (212) are arranged on the break-make that gas circuit is controlled on airtight pipeline (214),
What solenoid valve (212) and diaphragm pump (211) were electrically connected with pressure sensor (213) and were fed back according to pressure sensor (213)
Pressure information control running.
9. circulating tumor cell detecting instrument according to claim 6, which is characterized in that the fluid path silicone tube (222)
On be provided with electromagnet disabling mechanism (221) control fluid path break-make.
10. the circulating tumor cell detecting instrument according to claims 6, which is characterized in that the micro-fluidic chip 1
It is removably mounted on circulating tumor cell detecting instrument by slot (227).
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| CN109100286A (en) * | 2018-10-31 | 2018-12-28 | 江苏卓微生物科技有限公司 | cell counter |
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| CN110982665A (en) * | 2019-11-22 | 2020-04-10 | 上海理工大学 | Multi-channel sample introduction device and method for sorting and detecting circulating tumor cells |
| CN111019998A (en) * | 2019-12-26 | 2020-04-17 | 广东省医疗器械研究所 | Method and device for immediately detecting heterogeneous circulating tumor cells |
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| CN112080393A (en) * | 2020-09-29 | 2020-12-15 | 深圳睿思生命科技有限公司 | Automatic separation and enrichment device and method for tumor cells |
| CN112268934A (en) * | 2020-09-16 | 2021-01-26 | 东南大学 | Detection chip and detection method for circulating tumor cells |
| WO2022036891A1 (en) * | 2020-08-19 | 2022-02-24 | 东南大学 | Device for sorting and testing cancer cells |
| WO2022068648A1 (en) * | 2020-09-30 | 2022-04-07 | 苏州莱博睿思生物科技有限公司 | Microfluidic chip cartridge |
| WO2022226920A1 (en) * | 2021-04-29 | 2022-11-03 | 京东方科技集团股份有限公司 | Immunoassay device and immunoassay method |
| CN116875440A (en) * | 2023-06-21 | 2023-10-13 | 无锡百泰克生物技术有限公司 | Multiple nucleic acid detector, test paper and method based on chromatography |
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