CN110982665A - Multi-channel sample introduction device and method for sorting and detecting circulating tumor cells - Google Patents
Multi-channel sample introduction device and method for sorting and detecting circulating tumor cells Download PDFInfo
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Abstract
The invention relates to a multi-channel sample introduction device and a multi-channel sample introduction method for sorting and detecting circulating tumor cells. Including air pump, the control unit, command input and data display module, flow monitoring module, the module of ventilating and micro-fluidic chip, command input and data display module are used for the programming control to advance kind speed and passageway selection, the control unit links to each other with the air pump and the module of ventilating, the control unit is arranged in making the gas pressure in the air pump can accurate control, flow monitoring module links to each other with the module of ventilating and micro-fluidic chip, flow monitoring module is arranged in monitoring pipeline internal liquid velocity when the malleation advances kind to the velocity of flow of liquid among the control micro-fluidic chip, and negative feedback advances kind speed and liquid flow, the module of ventilating is used for the selection of multichannel and advances kind simultaneously to the multichannel. Compared with the prior art, the device and the method are simple and feasible to operate, and solve the problems of single channel, low stability and the like in the prior art.
Description
Technical Field
The invention relates to a sample introduction device, in particular to a multi-channel sample introduction device and a sample introduction method for sorting and detecting circulating tumor cells.
Background
Circulating Tumor Cells (CTC) have diagnostic value, but are contained in extremely low blood levels in patients with tumors, so that the requirements on the sorting sensitivity and specificity are high; in recent years, single cells in solid tumor tissues also have scientific research values of tumor heterogeneity and immune microenvironment, and become research hotspots in the field.
For detection, control of the injected process fluid is one of the key technologies of the droplet preparation system, and the amount, shape and manner of introducing the sample can affect the subsequent sample processing, because the chip system is small, and the effect is even crucial. Therefore, a stable and reliable fluid control module is important.
Currently, there are many types of drive and control techniques for microfluidics. Conventional driving methods based on static electricity, piezoelectricity, heat, and stepping motors are commonly used. These automatic sample feeding devices have certain limitations, such as the defects of difficult processing and integration, difficult precise control, slow sample feeding and discharging speed and the like; or the defects of complex structure, large volume, heavy weight, high cost, difficulty in miniaturization and the like of the device exist, and the injection pump is very weak when realizing nano-liter fluid, so that a series of problems of lag, long stabilization time, poor repeatability, pulse effect and the like often occur, and the application requirement is difficult to meet.
Therefore, the pressure driving technology is adopted, and the development of a sample feeding device with better comprehensive performance is an effective way for solving the problems.
Disclosure of Invention
The invention aims to provide a multi-channel sample introduction device and a multi-channel sample introduction method for sorting and detecting circulating tumor cells, and aims to solve the problems of single channel, low stability and the like in the prior art. The device and the method can accurately control the flow velocity and the flow of the liquid in the micro-fluidic chip, thereby ensuring that the circulating tumor cell sample liquid can be smoothly sorted and enriched on the micro-fluidic chip and the subsequent cell optical detection.
The purpose of the invention can be realized by the following technical scheme:
the invention provides a multi-channel sample introduction device for sorting and detecting circulating tumor cells, which comprises an air pump, a control unit, a command input and data display module, a flow monitoring module, an aeration module and a micro-fluidic chip,
the command input and data display module is respectively and electrically connected with the control unit, the ventilation module and the flow monitoring module, the command input and data display module is used for programming control of sample introduction speed and channel selection,
the control unit is connected with the air pump and the ventilation module and is used for accurately controlling the air pressure in the air pump,
the flow monitoring module is connected with the ventilation module and the micro-fluidic chip, and is used for monitoring the flow velocity of liquid in the pipeline during positive pressure sample introduction so as to control the flow velocity of the liquid in the micro-fluidic chip and negatively feeding back the sample introduction velocity and the liquid flow,
the ventilation module is used for selecting multiple channels and carrying out simultaneous sample injection on the multiple channels.
In an embodiment of the present invention, the control unit includes a precision pressure regulating valve, the ventilation module includes a multi-channel separation tube, a switching solenoid valve, and a gas-liquid action device, the gas pump is connected to the precision pressure regulating valve through a gas pipeline, the precision pressure regulating valve is connected to the multi-channel separation tube through a gas pipeline, the multi-channel separation tube is connected to the switching solenoid valve through a gas pipeline, the switching solenoid valve is connected to the gas-liquid action device through a gas pipeline, and the gas-liquid action device is connected to the microfluidic chip through a liquid pipeline.
In an embodiment of the present invention, the air pump includes a positive pressure air pump and an air filter, the positive pressure air pump and the air filter are connected through an air pipeline, the air filter is connected with the precision pressure regulating valve through an air pipeline, and the air filter is configured to filter out impurities such as water vapor and dust generated during the air pumping process of the positive pressure air pump 101, so as to ensure the dryness of the air during sample injection.
In one embodiment of the invention, a liquid pipeline between the gas-liquid action device and the microfluidic chip is connected with a flow sensor through an inverted cone connector, and the flow sensor is used for monitoring the sample introduction speed of the sample liquid;
and a pressure sensor is connected on a gas pipeline between the switch electromagnetic valve and the gas-liquid action device and is used for monitoring gas driving pressure.
In one embodiment of the present invention, the flow sensor and the gas pressure sensor are both connected to a data acquisition card through data lines, and the data acquisition card is used for acquiring data of gas pressure and liquid flow.
In one embodiment of the present invention, a gas pressure sensor signal amplifier is connected to a wire between the gas pressure sensor and the data acquisition card, and the gas pressure sensor signal amplifier is used for amplifying a weak gas pressure electrical signal.
In one embodiment of the present invention, the control unit further comprises an embedded processor, the embedded processor is respectively connected to the precise pressure regulating valve and the on-off solenoid valve through wires, controls the precise pressure regulating valve through a voltage signal to control the output of the driving gas pressure, and controls the on-off solenoid valve to control the selection of multiple channels.
In one embodiment of the present invention, the command input and data display module is connected to the data acquisition card and the embedded processor through a USB cable, and the command input and data display module is an upper computer, such as a computer.
In one embodiment of the present invention, the multi-channel separation tube is a device including one inlet channel and a plurality of outlet channels, the number of the switch solenoid valves is the same as the number of the outlet channels of the multi-channel separation tube, and each switch solenoid valve is connected to the outlet channel of one multi-channel separation tube; the number of the gas-liquid action devices is the same as that of the switch electromagnetic valves, and each gas-liquid action device is connected with one switch electromagnetic valve.
In one embodiment of the present invention, the gas-liquid action device is a closed device filled with the sample liquid, and the gas-liquid action device drives the sample liquid in the gas-liquid action device to enter the microfluidic chip at a stable speed by using the air pressure generated by the gas entering through the precise pressure regulating valve, the multi-channel separation tube and the on-off solenoid valve in sequence.
In one embodiment of the invention, the gas pipelines all use PVC gas guide tubes, the liquid pipelines all use Tygon capillaries, the connection parts of the PVC gas guide tubes are all connected by straight pagoda joints to ensure the tightness of the gas circuit, and the Tygon capillaries are connected with the inlet of the microfluidic chip through steel needles.
The air pump comprises a positive pressure inflating pump and an air filter, the control unit comprises a data acquisition card, an embedded processor, an air pressure sensor signal amplifier, an air pressure sensor and a precise pressure regulating valve, the flow monitoring module comprises an inverted cone connector, a Tygon capillary tube and a flow sensor, the ventilation module comprises a straight pagoda connector, a multi-channel separation tube, a switch electromagnetic valve, a PVC air guide tube, a gas-liquid action device bracket and a gas-liquid action device.
In one embodiment of the present invention, the gas-liquid action device is placed on a gas-liquid action device holder.
In the invention, the positive pressure inflating pump, the air filter, the data acquisition card, the embedded processor, the signal amplifier of the gas pressure sensor, the precise pressure regulating valve, the inverted cone connector, the Tygon capillary tube, the flow sensor, the straight-through pagoda connector, the multi-channel separation tube, the switch electromagnetic valve, the PVC gas guide tube, the gas-liquid action device bracket, the gas-liquid action device and the microfluidic chip all adopt the prior art.
In one embodiment of the present invention, the air pump, the control unit, the command input and data display module, the flow monitoring module, the ventilation module, and the microfluidic chip are all disposed on the console.
In one embodiment of the present invention, the electrical connection includes a connection via a USB cable.
In the invention, the ventilation module is provided with a switch electromagnetic valve and a multi-channel separation tube so as to realize programmed control of channel selection and multi-channel simultaneous sample injection.
In the invention, the precise pressure reducing valve in the control unit can be controlled manually and by a program, and the diversification of the control gas pressure can be realized.
The invention also provides a sample introduction method based on the multi-channel sample introduction device for sorting and detecting the circulating tumor cells, which comprises the following steps:
1) turning on a power switch of the equipment, initializing the equipment, and selecting an inlet channel, wherein the interface has 0-3 total sample introduction channels;
2) selecting the number of sample introduction channels according to the actual needs of a user;
3) after the channel is selected, setting the actually required sample introduction speed and sample introduction amount by a user according to the sample introduction speed and sample introduction amount prompted by the display module, reminding the user whether to confirm the input data by the input module, and returning to the step 2 if the selection is not; if so, starting sample introduction, and displaying the flow and the flow speed of the liquid in the pipeline in real time by a display module;
4) the command input and data display module provides a command for stopping and pausing sample injection, and can pause and stop the current sample injection.
Compared with the prior art, the invention has the following advantages and characteristics.
1. The working principle of the invention is that the air pump is used as the air source of the pressure driving device, the upper computer controls the precise pressure regulating valve by controlling the output of the voltage signal of the embedded processor, when the air of the air source enters the closed gas-liquid action device through the precise pressure regulating valve, the air pressure in the closed gas-liquid action device is accurately regulated and controlled, then the sample liquid in the gas-liquid action device is driven to enter the micro-fluidic chip at a stable speed, the air pressure sensor and the liquid flow sensor monitor the air pressure and the liquid flow in real time, and the liquid flow is used as the feedback quantity to realize the closed-loop flow output of the system.
2. The invention can realize multichannel selection and multichannel simultaneous sample injection, the flow monitoring module can negatively feed back the liquid flow and flow velocity in the microfluidic chip in real time, a closed-loop system is realized, the operation method is simple, and the highly integrated, miniaturized and closed-loop pressure-driven sample injection system with complex functions is realized.
Drawings
FIG. 1 is a block diagram of the structure of a multi-channel sampling device for sorting and detecting circulating tumor cells in embodiment 1 of the present invention;
FIG. 2 is a schematic diagram of the multi-channel sampling device for sorting and detecting circulating tumor cells in example 1 of the present invention;
FIG. 3 is a schematic diagram of the structure of a multi-channel sample injection device for sorting and detecting circulating tumor cells in example 1 of the present invention.
The air pump 1, the positive pressure inflating pump 101, the air filter 102, the control unit 2, the data acquisition card 201, the embedded processor 202, the gas pressure sensor signal amplifier 203, the gas pressure sensor 204, the precision pressure regulating valve 205, the command input and data display module 3, the flow monitoring module 4, the inverted cone connector 401, the Tygon capillary tube 402, the flow sensor 403, the ventilation module 5, the straight pagoda connector 501, the multi-channel separation tube 502, the switch electromagnetic valve 503, the PVC gas guide tube 504, the gas-liquid action device bracket 505, the gas-liquid action device 506, the micro-fluidic chip 6 and the console 7 are shown in the figure.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
Example 1
Referring to fig. 1-3, a multi-channel sample introduction device for sorting and detecting circulating tumor cells, which comprises an air pump 1, a control unit 2, a command input and data display module 3, a flow monitoring module 4, a ventilation module 5 and a microfluidic chip 6, wherein the command input and data display module 3 is electrically connected with the control unit 2, the ventilation module 5 and the flow monitoring module 4 respectively, the command input and data display module 3 is used for programming control of sample introduction speed and channel selection, the control unit 2 is connected with the air pump 1 and the ventilation module 5, the control unit 2 is used for enabling the gas pressure in the air pump 1 to be accurately controlled, the flow monitoring module 4 is connected with the ventilation module 5 and the microfluidic chip 6, the flow monitoring module 4 is used for monitoring the flow rate of liquid in a pipeline during positive pressure introduction to control the flow rate of liquid in the microfluidic chip 6, and the negative feedback sample introduction speed and the liquid flow rate, and the ventilation module 5 is used for selecting multiple channels and performing simultaneous sample introduction of the multiple channels.
In this embodiment, the control unit 2 includes the precision pressure regulating valve 205, the ventilation module 5 includes a multi-channel separation tube 502, a switching solenoid valve 503 and a gas-liquid action device 506, the air pump 1 is connected to the precision pressure regulating valve 205 through a gas pipeline, the precision pressure regulating valve 205 is connected to the multi-channel separation tube 502 through a gas pipeline, the multi-channel separation tube 502 is connected to the switching solenoid valve 503 through a gas pipeline, the switching solenoid valve 503 is connected to the gas-liquid action device 506 through a gas pipeline, and the gas-liquid action device 506 is connected to the microfluidic chip 6 through a liquid pipeline.
In this embodiment, air pump 1 includes malleation inflating pump 101 and air cleaner 102, malleation inflating pump 101 passes through the gas line with air cleaner 102 and links to each other, link to each other through the gas line between air cleaner 102 and the precision pressure-regulating valve 205, air cleaner 102 is used for filtering impurity such as vapor, dust that malleation inflating pump 101 inflation in-process produced, can guarantee the drying of gaseous when advancing the appearance.
In this embodiment, a liquid pipeline between the gas-liquid action device 506 and the microfluidic chip 6 is connected with a flow sensor 403 through an inverted cone connector 401, and the flow sensor 403 is used for monitoring the sample introduction speed of the sample liquid; a pressure sensor 204 is connected to the gas line between the switching solenoid valve 503 and the gas-liquid action device 506, and the pressure sensor 204 is used for monitoring the gas driving pressure.
In this embodiment, the flow sensor 403 and the gas pressure sensor 204 are both connected to the data acquisition card 201 through data lines, and the data acquisition card 201 is used for acquiring data of gas pressure and liquid flow.
In this embodiment, a wire between the gas pressure sensor 204 and the data acquisition card 201 is connected to a gas pressure sensor signal amplifier 203, and the gas pressure sensor signal amplifier 203 is configured to amplify a weak gas pressure electrical signal.
In this embodiment, the control unit 2 further includes an embedded processor 202, the embedded processor 202 is respectively connected to the precision pressure regulating valve 205 and the on-off solenoid valve 503 through wires, the precision pressure regulating valve 205 is controlled by a voltage signal to control the output of the driving gas pressure, and the on-off solenoid valve 503 is controlled to control the selection of multiple channels.
In this embodiment, the command input and data display module 3 is connected to the data acquisition card 201 and the embedded processor 202 via USB cables, and the command input and data display module 3 is an upper computer, such as a computer.
In this embodiment, the multi-channel separation tube 502 includes an inlet channel and a plurality of outlet channels, the number of the switch solenoid valves 503 is the same as the number of the outlet channels of the multi-channel separation tube 502, and each switch solenoid valve 503 is connected to one outlet channel of the multi-channel separation tube 502; the number of the gas-liquid action devices 506 is the same as the number of the on-off solenoid valves 503, and each of the gas-liquid action devices 506 is connected to one of the on-off solenoid valves 503.
In this embodiment, the gas-liquid action device 506 is a closed device filled with sample liquid, and the gas-liquid action device 506 drives the sample liquid in the gas-liquid action device 506 to enter the microfluidic chip 6 at a stable speed by using the air pressure generated by the gas entering through the precision pressure regulating valve 205, the multi-channel separation tube 502, and the on-off electromagnetic valve 503 in sequence.
In this embodiment, the gas pipelines all use PVC gas-guide tubes 504, the liquid pipelines all use Tygon capillaries 402, the junctions of the PVC gas-guide tubes 504 are all connected by straight pagoda joints 501 to ensure the air-tightness of the gas circuit, and the Tygon capillaries 402 are connected with the inlet of the microfluidic chip 6 by steel needles.
In this embodiment, the gas-liquid action device 506 is placed on the gas-liquid action device holder 505.
In this embodiment, the air pump 1 includes a positive pressure inflating pump 101 and an air filter 102, the control unit 2 includes a data acquisition card 201, an embedded processor 202, a signal amplifier 203 of an air pressure sensor, an air pressure sensor 204, a precision pressure regulating valve 205, the flow monitoring module 4 includes an inverted cone connector 401, a Tygon capillary tube 402, a flow sensor 403, and the ventilation module 5 includes a straight pagoda connector 501, a multi-channel separation tube 502, a switch solenoid valve 503, a PVC airway 504, a gas-liquid action device bracket 505, and a gas-liquid action device 506.
In this embodiment, the positive pressure inflating pump 101, the air filter 102, the data acquisition card 201, the embedded processor 202, the gas pressure sensor signal amplifier 203, the gas pressure sensor 204, the precise pressure regulating valve 205, the inverted cone connector 401, the Tygon capillary tube 402, the flow sensor 403, the straight pagoda connector 501, the multi-channel separation tube 502, the switch electromagnetic valve 503, the PVC gas-guide tube 504, the gas-liquid action device bracket 505, the gas-liquid action device 506, and the microfluidic chip 6 all adopt the prior art.
In this embodiment, the air pump 1, the control unit 2, the command input and data display module 3, the flow monitoring module 4, the ventilation module 5, and the microfluidic chip 6 are all disposed on the console 7.
In this embodiment, the ventilation module is provided with a switch solenoid valve and a multi-channel separation tube to realize programmed control of channel selection and simultaneous multi-channel sample injection. The precise pressure reducing valve in the control unit can be controlled manually and in a program mode, and the diversification of the pressure of the control gas can be realized.
The embodiment also provides a sample introduction method of the multi-channel sample introduction device for sorting and detecting the circulating tumor cells, which comprises the following steps:
1) turning on a power switch of the equipment, initializing the equipment, and selecting an inlet channel, wherein the interface has 0-3 total sample introduction channels;
2) selecting the number of sample introduction channels according to the actual needs of a user;
3) after the channel is selected, setting the actually required sample introduction speed and sample introduction amount by a user according to the sample introduction speed and sample introduction amount prompted by the display module, reminding the user whether to confirm the input data by the input module, and returning to the step 2 if the selection is not; if so, starting sample introduction, and displaying the flow and the flow speed of the liquid in the pipeline in real time by a display module;
4) the command input and data display module provides a command for stopping and pausing sample injection, and can pause and stop the current sample injection.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (10)
1. A multi-channel sample introduction device for sorting and detecting circulating tumor cells is characterized by comprising an air pump (1), a control unit (2), a command input and data display module (3), a flow monitoring module (4), an aeration module (5) and a micro-fluidic chip (6),
the command input and data display module (3) is respectively and electrically connected with the control unit (2), the ventilation module (5) and the flow monitoring module (4), the command input and data display module (3) is used for controlling the sampling speed and channel selection in a programmed way,
the control unit (2) is connected with the air pump (1) and the ventilation module (5), the control unit (2) is used for enabling the air pressure in the air pump (1) to be accurately controlled,
the flow monitoring module (4) is connected with the ventilation module (5) and the microfluidic chip (6), the flow monitoring module (4) is used for monitoring the flow velocity of liquid in the pipeline during positive pressure sample introduction so as to control the flow velocity of the liquid in the microfluidic chip (6) and negatively feed back the sample introduction speed and the liquid flow,
the ventilation module (5) is used for selecting multiple channels and carrying out simultaneous sample injection on the multiple channels.
2. The multichannel sampling device for sorting and detecting circulating tumor cells according to claim 1, wherein the control unit (2) comprises a precision pressure regulating valve (205), the ventilation module (5) comprises a multichannel separating tube (502), a switching solenoid valve (503) and a gas-liquid action device (506), the air pump (1) is connected with the precision pressure regulating valve (205) through a gas pipeline, the precision pressure regulating valve (205) is connected with the multichannel separating tube (502) through a gas pipeline, the multichannel separating tube (502) is connected with the switching solenoid valve (503) through a gas pipeline, the switching solenoid valve (503) is connected with the gas-liquid action device (506) through a gas pipeline, and the gas-liquid action device (506) is connected with the microfluidic chip (6) through a liquid pipeline.
3. The multi-channel sampling device for sorting and detecting circulating tumor cells according to claim 2, wherein the air pump (1) comprises a positive pressure air pump (101) and an air filter (102), the positive pressure air pump (101) is connected with the air filter (102) through an air pipeline, and the air filter (102) is connected with the precision pressure regulating valve (205) through an air pipeline.
4. The multi-channel sample introduction device for sorting and detecting circulating tumor cells according to claim 2, wherein a flow sensor (403) is connected to a liquid pipeline between the gas-liquid action device (506) and the microfluidic chip (6), and the flow sensor (403) is used for monitoring the sample introduction speed of the sample liquid;
a pressure sensor (204) is connected to a gas pipeline between the switch electromagnetic valve (503) and the gas-liquid action device (506), and the pressure sensor (204) is used for monitoring gas driving pressure.
5. The multi-channel sampling device for sorting and detecting circulating tumor cells according to claim 4, wherein the flow sensor (403) and the gas pressure sensor (204) are connected to a data acquisition card (201) through data lines, and the data acquisition card (201) is used for acquiring data of gas pressure and liquid flow.
6. The multi-channel sampling device for sorting and detecting circulating tumor cells according to claim 5, wherein a gas pressure sensor signal amplifier (203) is connected to a wire between the gas pressure sensor (204) and the data acquisition card (201), and the gas pressure sensor signal amplifier (203) is used for amplifying a gas pressure electrical signal.
7. The multi-channel sampling device for sorting and detecting circulating tumor cells according to claim 6, wherein the control unit (2) further comprises an embedded processor (202),
the embedded processor (202) is respectively connected with the precision pressure regulating valve (205) and the switch electromagnetic valve (503) through leads, controls the precision pressure regulating valve (205) through voltage signals to control the output of driving gas pressure, and controls the switch electromagnetic valve (503) to control the selection of multiple channels;
the command input and data display module (3) is connected with the data acquisition card (201) and the embedded processor (202) through USB lines, and the command input and data display module (3) is a computer.
8. The multi-channel sample introduction device for sorting and detecting circulating tumor cells according to claim 2, wherein the multi-channel separation tube (502) comprises an inlet channel and a plurality of outlet channels, the number of the on-off solenoid valves (503) is the same as that of the outlet channels of the multi-channel separation tube (502), and each on-off solenoid valve (503) is respectively connected with the outlet channel of one multi-channel separation tube (502);
the number of the gas-liquid action devices (506) is the same as that of the switch electromagnetic valves (503), and each gas-liquid action device (506) is connected with one switch electromagnetic valve (503).
9. The multi-channel sample introduction device for sorting and detecting circulating tumor cells according to claim 2, wherein the gas-liquid action device (506) is a closed device filled with sample liquid, and the sample liquid in the gas-liquid action device (506) is driven by the gas pressure generated by gas entering through the precise pressure regulating valve (205), the multi-channel separation tube (502) and the on-off solenoid valve (503) in sequence to enter the microfluidic chip (6) at a stable speed by the gas pressure.
10. A sample introduction method based on the device according to any of claims 1-9, characterized by comprising the steps of:
1) turning on a power switch of the equipment, initializing the equipment, and selecting an inlet channel, wherein the interface has 0-3 total sample introduction channels;
2) selecting the number of sample introduction channels according to the actual needs of a user;
3) after the channel is selected, setting the actually required sample introduction speed and sample introduction amount by a user according to the sample introduction speed and sample introduction amount prompted by the display module, reminding the user whether to confirm the input data by the input module, and returning to the step 2 if the selection is not; if so, starting sample introduction, and displaying the flow and the flow speed of the liquid in the pipeline in real time by a display module;
4) the command input and data display module provides a command for stopping and pausing sample injection, and can pause and stop the current sample injection.
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Cited By (4)
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| CN112481077A (en) * | 2020-12-01 | 2021-03-12 | 北京理工大学 | Microfluidic perfusion culture device and perfusion method thereof |
| WO2022062934A1 (en) * | 2020-09-22 | 2022-03-31 | 深圳市瑞格生物科技有限公司 | Microfluidic chip-based circulating tumor/fusion cell capturing device and method |
| CN114558631A (en) * | 2022-03-17 | 2022-05-31 | 中南大学 | Microfluidic device, microfluidic sample input system and control method |
| CN114755356A (en) * | 2022-05-17 | 2022-07-15 | 江苏炫一科学仪器有限公司 | Microflow plate multichannel gas sample selecting and sampling device |
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| CN112481077B (en) * | 2020-12-01 | 2022-11-08 | 北京理工大学 | Microfluidic perfusion culture device and perfusion method thereof |
| CN114558631A (en) * | 2022-03-17 | 2022-05-31 | 中南大学 | Microfluidic device, microfluidic sample input system and control method |
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| CN114755356B (en) * | 2022-05-17 | 2024-05-07 | 江苏炫一科学仪器有限公司 | Microfluidic plate multipath gas sample selection sampling device |
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