CN101692048A - Microchip analysis system for capillary electrophoresis separation and chemiluminescence detection - Google Patents
Microchip analysis system for capillary electrophoresis separation and chemiluminescence detection Download PDFInfo
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- CN101692048A CN101692048A CN200910154433A CN200910154433A CN101692048A CN 101692048 A CN101692048 A CN 101692048A CN 200910154433 A CN200910154433 A CN 200910154433A CN 200910154433 A CN200910154433 A CN 200910154433A CN 101692048 A CN101692048 A CN 101692048A
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Abstract
The invention provides a microchip analysis system integrated capillary electrophoresis separation with chemiluminescence detection, which consists of a microfluidic chip, a single-path high voltage power source, a minipump, a three-way valve, an interface, a needle adjusting valve, a U-tube type monometer, a photomultiplier, a vacuum flask, an electric contact vacuum gauge and a time relay. The microchip analysis system provided by the invention has simple structure, no mutual interference of the capillary electrophoresis separation and chemiluminescence detection, and high separation efficiency of the capillary electrophoresis and high sensitivity of the chemiluminescence, has the characteristics of high separation efficiency, high detection sensitivity, small volume, light weight, convenient operation, low cost and the like, and is an ideal portable microchip analysis system.
Description
Technical field
The present invention relates to micro-total analysis system, particularly relate to the portable microchip analytic system of integrated chip capillary electrophoresis separation and chemiluminescence detection.
Background technology
Since nineteen ninety proposed the micro-total analysis system notion, the micro-fluidic chip technology had been opened up wide development space in fields such as chemistry, life science, environmental science and Food Sciences.Since chip capillary cataphoresis have separation efficiency height, portable, reagent consumption less, advantage such as analysis time is short, be isolation technics commonly used in the microchip analysis system.But the detection volume of chip capillary cataphoresis is little, light path is short, must have high sensitivity with the detecting device of its coupling.
Because laser induced fluorescence detector is highly sensitive, little with the technical difficulty of chip capillary cataphoresis coupling, therefore, up to the present, laser induced fluorescence detector is used widely in the micro-fluidic chip analytic system, and it also is unique adopted detecting device in commercialization micro-fluidic chip analytic system at present.But the laser induced fluorescence detector volume is bigger, and structure is complicated, cost is higher, is difficult to microminiaturization.
Chemiluminescence detecting method because of it need not excitation source, simple in structure, highly sensitive and be easy to characteristics such as microminiaturization, is one of the most attractive detection method of chip capillary cataphoresis in theory.But chip capillary cataphoresis need adopt the split tunnel of multiple power supplies with the sample introducing micro-fluidic chip of skin upgrading, carries out compartment analysis.And chemoluminescence method needs the extra syringe pump of addition, peristaltic pump or electroosmotic flow (pump) that chemical illuminating reagent is introduced sense channel, by chemiluminescence reaction takes place in sense channel, could carry out qualitative and quantitative analysis to determinand with analysans after chip capillary cataphoresis separates.Because these pumps and multiple power supplies cost an arm and a leg, volume is big, thereby has increased the cost and the microminiaturized difficulty of microchip analysis system.And the flow velocity and the flow direction that must strict control luminescence reagent when experimental implementation can not flow backwards it and enter the split tunnel of chip capillary cataphoresis and influence separation efficiency.But because the network structure of micro-fluidic chip, operation easier is very big.Therefore, compare with laser induced fluorescence detector, chemiluminescence detector is not widely used in the micro-fluidic chip analytic system, does not see the commercialization instrument of integrated chip capillary electrophoresis separation and chemiluminescence detection yet.
Summary of the invention
One of purpose of the present invention is for a kind of easy to operate, simple in structure, volume is little and price the is low integrated chip capillary electrophoresis separation and the portable microchip analytic system of chemiluminescence detection are provided, and two of purpose is to utilize microchip analysis system of the present invention to carry out capillary electrophoresis separation and chemiluminescence detection.
The microchip analysis system of integrated chip capillary electrophoresis separation provided by the invention and chemiluminescence detection is made up of micro-fluidic chip, single channel high-voltage power supply, minipump, T-valve, aciculiform variable valve, U type pipe pressure meter, photomultiplier, Dewar bottle, electric contact vacuum meter and the time relay; Microchannel in the described micro-fluidic chip can be divided into sample intake passage by function, split tunnel, sheath circulation road and sense channel; Passage between sample intake passage and split tunnel joining and split tunnel and the sheath circulation road joining is effective split tunnel, effectively in the split tunnel micropore plug is arranged; Passage between split tunnel terminal and the waste liquid pool is a sense channel; Photomultiplier directly places the sense channel below, is used to detect the light intensity that chemiluminescence reaction is sent; Two ends of each microchannel have liquid storage tank, and they are respectively damping fluid liquid storage tank, sample liquid storage tank, sample waste liquid pool, sheath stream liquid storage tank, waste liquid pool; Negative pressure source is connected and composed by Dewar bottle and electric contact vacuum meter and minipump; Dewar bottle is connected with a port of miniature three-way valve by connecting pipe, the c port of miniature three-way valve directly communicates with atmosphere, the public port b of T-valve links to each other with the sample waste liquid pool with silicone rubber tube by polyfluortetraethylene pipe, insert the polyfluortetraethylene pipe of waste liquid pool and the liquid level that silicone rubber tube is higher than the electrophoretic buffer in the waste liquid pool, remain not with the liquid level of electrophoretic buffer in the sample waste liquid pool and contact, guarantee the impermeability of interface simultaneously; One end of needle type regulating valve is connected with waste liquid pool, and the other end of needle type regulating valve is connected with Dewar bottle; The time relay is accurately controlled the time in sample introduction stage.
Microchip analysis system electrophoretic separation electric field of the present invention is connected between damping fluid liquid storage tank and one of them sheath stream liquid storage tank, because the electrophoretic separation electric field that the single channel high-voltage power supply is applied is without sense channel, the stability of electrophoretic separation and result's favorable reproducibility, and be suitable for various chemical luminous systems, comprise nonconducting non-aquoluminescence system.When chemical illuminating reagent is nonconducting non-aqueous solution, the non-aqueous solution of chemical illuminating reagent is flowed in a sheath circulation road therein, and add electrophoretic buffer, and the separation electric field of chip capillary cataphoresis is applied on the split tunnel by the sheath circulation road that electrophoretic buffer flows through at another sheath circulation road.When chemical illuminating reagent is the aqueous solution of conduction, the separation electric field of chip capillary cataphoresis is applied on the split tunnel by the sheath circulation road that chemical illuminating reagent flows through.
Microchip analysis system of the present invention makes in the Dewar bottle by minipump and electric contact vacuum meter and produces and keep stable negative pressure, negative pressure in the Dewar bottle has two effects, A. the negative pressure in the Dewar bottle combines with single channel high-voltage power supply and miniature three-way valve, the sample introduction and the operation that separates of control micro flow control chip capillary electrophoresis; B. after the negative pressure in the Dewar bottle is regulated negative pressure by needle type regulating valve, make chemical illuminating reagent pass through sense channel with constant flow velocity in the whole analysis phase, mix the back luminescence-producing reaction that in sense channel, takes place mutually with the component to be analyzed after chip capillary cataphoresis separates, obtain corresponding electrophoresis peak, according to electrophoresis peak transit time and peak height, component to be measured is carried out qualitative and quantitative analysis.
Microchip analysis system of the present invention applies a subatmospheric negative pressure by the opening degree of control aciculiform variable valve above waste liquid pool, make in the sheath stream liquid storage tank luminescence reagent by the sheath circulation road after testing passage flow to waste liquid pool; By regulating the opening degree of aciculiform variable valve, the flow velocity of each component in sense channel and the width at electrophoresis peak after control luminescence reagent and the electrophoretic separation.Thereby the opertaing device of chip capillary cataphoresis separation and chemiluminescence detection combined apparatus and operation are simplified greatly, and volume dwindles greatly.
Microchip analysis system of the present invention is applied to the negative pressure of waste liquid pool top with the instrument monitoring of U type pipe pressure meter or mensuration vacuum tightness.
Microchip analysis system of the present invention is by the sample introduction stage or the separation phase of the valve position control chip Capillary Electrophoresis of T-valve; The valve position of T-valve when controlling with electrical means, is accurately controlled the time in sample introduction stage with electronic and manual two kinds of methods control with the time relay.T-valve comprises: three-way solenoid valve, by motor-driven T-valve and manual three-way valve.
The micro-fluidic chip that microchip analysis system of the present invention uses has been made the micropore plug in split tunnel.This micropore plug is very big to the resistance of pressure current, and very little to the electroosmotic flow resistance, therefore analyzed sample can be under the driving of electroosmotic flow, and entering effective split tunnel carries out electrophoretic separation, and the negative pressure that can not be applied on the waste liquid pool sucks split tunnel, influences separation efficiency.Simultaneously, prevented that also the negative pressure that is applied on the sample waste liquid pool from sucking split tunnel with luminescence reagent, influences electrophoretic separation.
The specific operation process of microchip analysis system of the present invention is: by setting the minimum and maximum vacuum tightness of electric contact vacuum meter, connect the minipump power supply, make and form negative pressure in the Dewar bottle, when reaching to set on the vacuum tightness, vacuum tightness in the bottle prescribes a time limit, electric contact vacuum meter is closed the minipump power supply, prescribe a time limit when vacuum tightness in the bottle is lower than to set under the vacuum tightness, electric contact vacuum meter starts minipump, makes bottle interior vacustat in the scope of setting.
The operation of the microfluid control chip capillary electrophoresis analysing of microchip analysis system of the present invention by sample introduction with separate two stages and form.In the sample introduction stage, three-way solenoid valve public port b is communicated with a port.This moment, micro-fluidic chip sample waste liquid pool was connected with Dewar bottle.Under the effect of negative pressure, solution and the sample solution in the sample cell in the buffer pool all flow to the sample waste liquid pool by micro-fluidic chip crossing.Because the micropore plug of making in the split tunnel is very big to the resistance of pressure current, the negative pressure that applies above the sample waste liquid pool can not make the solution in sheath stream liquid storage tank and the waste liquid pool flow into the sample waste liquid pool by the microchannel.Meanwhile, sample solution produces at high voltage under the driving of electroosmotic flow when flowing through micro-fluidic chip crossing, enters in effective split tunnel, enters that sample plug length was directly proportional with the duration in sample introduction stage in effective split tunnel.At separation phase, three-way solenoid valve b end and c end are communicated with.Because the c of three-way solenoid valve end directly communicates with atmosphere, and the sample waste liquid pool is communicated with atmosphere, the pressure differential disappearance simultaneously immediately between it and other liquid pools.The sample plug that has entered effective split tunnel is added under the effect of the electric field force that electric field produces on the split tunnel, is effectively being separated in the split tunnel.By optimizing the liquid level of each liquid storage tank, can prevent that sample solution from entering split tunnel and influencing separating effect when separating.By controlling the opening degree of miniature aciculiform variable valve, can make luminescence reagent sample introduction with separate two stages and all flow into waste liquid pool through sense channel with constant flow velocity, component to be analyzed after chip capillary cataphoresis separates is mixed the generation chemiluminescence reaction with luminescence reagent in sense channel after split tunnel and sheath circulation road joining are met, thereby obtain corresponding electrophoresis peak, can carry out qualitative and quantitative analysis to component to be measured according to electrophoresis peak transit time and peak height.
Microchip analysis system provided by the invention, electrophoretic separation and chemiluminescence detection are not disturbed mutually, have guaranteed the high sensitivity of the high separating efficiency and the chemoluminescence method of Capillary Electrophoresis.Have separation efficiency height, detection sensitivity height, simple in structure, volume is little, the high separating efficiency in light weight, easy to operate, with low cost, as to have Capillary Electrophoresis concurrently and the highly sensitive characteristics of chemoluminescence method are to make the desirable coupling technique of the portable completely separating system that declines.
Description of drawings
The microchip analysis system synoptic diagram of Fig. 1 integrated chip capillary electrophoresis separation and chemiluminescence detection
Among the figure: 1-micro-fluidic chip, 2-micropore plug, 3-high-voltage power supply, the 4-silicone rubber tube, the 5-polyfluortetraethylene pipe, 6-U type pipe pressure meter, 7-needle type regulating valve, the 8-electric contact vacuum meter, the 9-Dewar bottle, 10-minipump, 11-photomultiplier, 12-T-valve and a, three ports of b, c, the 13-time relay.
The electrophoretogram of heavy metal ion in separation of Fig. 2 chip capillary cataphoresis and the chemiluminescence detection analysis water-like
Embodiment
Referring to Fig. 1, damping fluid liquid storage tank B, sample liquid storage tank S, sample waste liquid pool SW, sheath stream liquid storage tank L and H, waste liquid pool W are arranged on the micro-fluidic chip 1.Wherein, the micro-fluidic chip sample intake passage is S-SW, and split tunnel is B-P
2, sample intake passage and split tunnel intersect P
1, split tunnel and sheath circulation road intersect P
2, P
1And P
2Between passage be effective split tunnel P
1-P
2Effective split tunnel P
1-P
2In micropore plug 2 is arranged, sheath stream liquid storage tank L and H and split tunnel tie point P
2Between constitute sheath circulation road L-P
2With sheath circulation road H-P
2, split tunnel terminal P
2And the passage between the waste liquid pool W is sense channel P
2-W, photomultiplier directly place the sense channel below, are used to detect the light intensity that chemiluminescence reaction is sent.Negative pressure source is connected and composed by Dewar bottle 9 and electric contact vacuum meter 8 and minipump 10.Dewar bottle 9 is connected with a port of miniature three-way valve 12 by the teflon connecting pipe, the c port of T-valve 12 directly communicates with atmosphere, the b port links to each other with sample waste liquid pool SW with silicone rubber tube 4 by polyfluortetraethylene pipe 5, insert the polyfluortetraethylene pipe of waste liquid pool SW and silicone rubber tube and remain not with the liquid level of electrophoretic buffer in the SW liquid storage tank and contact, guarantee the impermeability of interface simultaneously.Using the same method connects needle type regulating valve 7 and waste liquid pool W, and the other end of needle type regulating valve 7 is connected with Dewar bottle 9.
Add sample solution among the sample liquid storage tank S on micro-fluidic chip, in sheath stream liquid storage tank L and H, add chemical illuminating reagent, in damping fluid liquid storage tank B, sample waste liquid pool SW, waste liquid pool W, add the electrophoretic buffer of different volumes.Keep the liquid level of the height of liquid level among the sample liquid storage tank S less than damping fluid liquid storage tank B, the liquid level among the sample waste liquid liquid storage tank SW is less than the height of liquid level among the sample liquid storage tank S.Apply+the 1200V high voltage sheath stream liquid storage tank L end ground connection at damping fluid liquid storage tank B end.
At first set the maximum vacuum and the minimum vacuum degree of electric contact vacuum meter.The b end and the c end of miniature three-way valve 12 are communicated with, and a end ends, and closes needle type regulating valve simultaneously.Open minipump 10, make and form negative pressure in the Dewar bottle 9, when reaching to set on the vacuum tightness, pressure of the inside of a bottle prescribes a time limit, electric contact vacuum meter 8 is closed minipump, when being lower than to set under the vacuum tightness, pressure of the inside of a bottle prescribes a time limit, electric contact vacuum meter starts minipump 10, makes bottle interior vacustat in setting range.
Negative pressure in the Dewar bottle has two effects, 1) and single channel high-voltage power supply 3 and T-valve 12 combine control chip Capillary Electrophoresis sample introduction and the operation that separates.2) reduce negative pressure by needle type regulating valve 7 after, make among sheath stream liquid storage tank L and the H chemical illuminating reagent sample introduction and separates two stages all with constant flow velocity through sense channel inflow waste liquid pool W, component to be analyzed after the chip capillary cataphoresis separation and luminescence reagent are at P
2Point meets the back in sense channel mixing generation luminescence-producing reaction, thereby obtains corresponding electrophoresis peak, can obtain component to be measured is carried out qualitative and quantitative analysis from electrophoresis peak transit time and peak height.
The operation that micro flow control chip capillary electrophoresis separates by sample introduction with separate two stages and form.In the sample introduction stage, the b end and a end of T-valve 12 are communicated with, Dewar bottle 9 is through communicating by polyfluortetraethylene pipe 5 and sample waste liquid pool SW, sample waste liquid pool top is formed less than atmospheric negative pressure, on the micro-fluidic chip among the liquid storage tank S among sample solution and the liquid storage tank B damping fluid under the effect of negative pressure, flow to sample waste liquid pool SW, because it is very big to the resistance of pressure current to have made effective split tunnel of micropore plug, and it is very little to the electroosmotic flow resistance, therefore make sample solution under the driving of electroosmotic flow, enter effective split tunnel P
1-P
2, and the flow of solution among sheath stream liquid storage tank L and H and the waste liquid pool W can not flow into the sample waste liquid pool by split tunnel.Meanwhile, sample solution is at the micro-fluidic chip crossing P that flows through
1During the place, be added under the driving of electric field between split tunnel, enter split tunnel, the sample plug length that enters split tunnel was directly proportional with the duration in sample introduction stage.
At separation phase, the b of T-valve 12 end and c end are communicated with.Because the c of T-valve 12 end directly communicates with atmosphere, thereby the sample waste liquid pool is communicated with atmosphere, pressure differential between it and other liquid pool disappears immediately simultaneously, the sample plug that enters split tunnel is added under the electroosmotic flow effect that electric field produced on the split tunnel brings split tunnel into, the beginning electrophoretic separation.Component to be measured after chip capillary cataphoresis separates and luminescence reagent are at P
2Point meets the back in sense channel mixing generation luminescence-producing reaction, obtains corresponding electrophoresis peak, obtains component to be measured is carried out qualitative and quantitative analysis from electrophoresis peak transit time and peak height.
Provide a example according to embodiment 1 with heavy metal ion in the microchip analysis system analysis water-like.Referring to Fig. 1, the passage on the micro-fluidic chip 1 between sample liquid storage tank S and the sample waste liquid pool SW is a sample intake passage, P
1And P
2Between passage be effective split tunnel, effective split tunnel P
1-P
2In micropore plug 2 is arranged, P
2And the passage between the W is sense channel, and photomultiplier directly places the sense channel below, is used to detect the light intensity that chemiluminescence reaction is sent.Respectively spiling at the passage two ends, is 5.0mm with three internal diameters respectively with epoxide-resin glue, and height is fixed on around hole S, SW, the B for the plastics liquid storage tank of 15.0mm; Three internal diameters are 7.0mm, and are high for the plastics liquid storage tank of 25.0mm is separately fixed at around hole L, H, the W, respectively as their liquid storage tank.Liquid storage tank S, SW, B, W are respectively applied for and hold sample, sample waste liquid, electrophoretic buffer and waste liquid, and liquid storage tank H, L are respectively applied for the solution that holds hydrogen peroxide and luminol.Liquid storage tank S, SW, B put P apart from right-angled intersection
1Distance be 5.0mm, liquid storage tank L, H are apart from point P
2Be 5.0mm; Split tunnel B-P
2Total length is 35.0mm, wherein effective separation length P
1-P
2Be 30.0mm.It is dark that the passage of micro-fluidic chip is 30 μ m, and 100 μ m are wide; Sense channel is a spirality, and its length is 26.6mm.Add sample solution among the sample liquid storage tank S on micro-fluidic chip, in L, add chemical illuminating reagent 1.0 * 10
-3The solution of the luminol of M adds the 0.05M superoxol among the H.Electrophoretic buffer (the 20mM NaAc+HAc pH 4.5+5mM α-HIBA) that adds different volumes at liquid storage tank B, SW and W.Keep the liquid level of the height of liquid level among the sample liquid storage tank S less than liquid storage tank B, the liquid level among the sample waste liquid liquid storage tank SW is less than the height of liquid level among the liquid storage tank S.Apply+the 1200V high voltage luminol solution ground connection in the L liquid storage tank at split tunnel B end.
Above waste liquid pool W, apply subatmospheric negative pressure, make among L and the H chemical illuminating reagent sample introduction with separate two stages and all flow into waste liquid pool W through sense channel with constant flow velocity.
The operation that micro flow control chip capillary electrophoresis separates by sample introduction with separate two stages and form.In the sample introduction stage, the b end and a end of T-valve 12 are communicated with, Dewar bottle 9 is through communicating by polyfluortetraethylene pipe 5 and sample waste liquid pool SW, above sample waste liquid pool SW, form subatmospheric negative pressure, sample solution on the micro-fluidic chip in S and the B liquid storage tank and damping fluid etc. flow to sample waste liquid pool SW under the effect of negative pressure, because it is very big to the resistance of pressure current to have made the split tunnel of micropore plug, and it is very little to the electroosmotic flow resistance, therefore make analyzed sample under the driving of electroosmotic flow, enter split tunnel P
1-P
2, and L, the flow of solution in H and the W liquid storage tank can not flow into the sample waste liquid pool by split tunnel.After 2 seconds, the b of switch three-way valve 12 end and c end are communicated with, and the sample waste liquid pool is communicated with atmosphere, the pressure differential disappearance simultaneously immediately between it and other liquid pool, the sample plug that enters split tunnel is added under the effect of the electroosmotic flow that electric field produces on the split tunnel, the beginning electrophoretic separation.Each component after the electrophoretic separation and chemical illuminating reagent hybrid concurrency hair tonic light reaction mutually in sense channel, the electrophoretogram that obtains is seen Fig. 2.
Claims (8)
1. the microchip analysis system of integrated chip capillary electrophoresis separation and chemiluminescence detection is made up of micro-fluidic chip, single channel high-voltage power supply, minipump, T-valve, aciculiform variable valve, U type pipe pressure meter, photomultiplier, Dewar bottle, electric contact vacuum meter and the time relay; It is characterized in that: the microchannel is divided into sample intake passage by function in the described micro-fluidic chip, split tunnel, sheath circulation road and sense channel; Passage between sample intake passage and split tunnel joining and split tunnel and the sheath circulation road joining is effective split tunnel, effectively in the split tunnel micropore plug is arranged, and the passage between split tunnel terminal and the waste liquid pool is a sense channel; Photomultiplier directly places the sense channel below, is used to detect the light intensity that chemiluminescence reaction is sent; Two ends of each passage have liquid storage tank, comprising: damping fluid liquid storage tank, sample liquid storage tank, sample waste liquid pool, sheath stream liquid storage tank, waste liquid pool; Negative pressure source is connected and composed by Dewar bottle and electric contact vacuum meter and minipump; Dewar bottle is connected with a port of T-valve by connecting pipe, another port of T-valve directly communicates with atmosphere, the public port of T-valve links to each other with the sample waste liquid pool with silicone rubber tube by polyfluortetraethylene pipe, insert the polyfluortetraethylene pipe of waste liquid pool and the liquid level that silicone rubber tube is higher than the electrophoretic buffer in the waste liquid pool, guarantee the impermeability of interface simultaneously; One end of needle type regulating valve is connected with waste liquid pool, and the other end of needle type regulating valve is connected with Dewar bottle; The time relay is accurately controlled the time in sample introduction stage.
2. microchip analysis system as claimed in claim 1, it is characterized in that used T-valve comprise three-way solenoid valve, by motor-driven T-valve and manual three-way valve.
3. microchip analysis system as claimed in claim 1 is used for the analytical approach of electrophoretic separation and chemiluminescence detection, it is characterized in that: the operation of microfluid control chip capillary electrophoresis analysing by sample introduction with separate two stages and form, in the sample introduction stage, the T-valve public port is communicated with a port, micro-fluidic chip sample waste liquid pool is connected with Dewar bottle, under the effect of negative pressure, solution and the sample solution in the sample cell in the buffer pool all flow to the sample waste liquid pool by micro-fluidic chip crossing; Sample solution produces at high voltage under the driving of electroosmotic flow when flowing through micro-fluidic chip crossing, enters in effective split tunnel; At separation phase, T-valve public port and another port are communicated with, the sample waste liquid pool is communicated with atmosphere, the sample plug that has entered effective split tunnel is added under the electrophoretic force effect that high voltage produces, and is separated at effective split tunnel; By controlling the negative pressure in the miniature aciculiform variable valve adjusting Dewar bottle, make luminescence reagent sample introduction with separate two stages and all flow into waste liquid pool through sense channel with constant flow velocity, component to be analyzed after chip capillary cataphoresis separates and luminescence reagent are after split tunnel and sheath circulation road joining are met, mix the generation chemiluminescence reaction at sense channel, obtain corresponding electrophoresis peak, according to electrophoresis peak transit time and peak height, component to be measured is carried out qualitative and quantitative analysis.
4. the analytical approach of microchip analysis system as claimed in claim 3, it is characterized in that: above waste liquid pool, apply a subatmospheric negative pressure by control aciculiform variable valve, make in the sheath stream liquid storage tank luminescence reagent by the sheath circulation road after testing passage flow to waste liquid pool; By regulating the opening degree of aciculiform variable valve, the flow velocity of each component in sense channel and the width at electrophoresis peak after control luminescence reagent and the electrophoretic separation.
5. the analytical approach of microchip analysis system as claimed in claim 3 is characterized in that: monitor the negative pressure that is applied to the waste liquid pool top with U type pipe pressure meter or the instrument of measuring vacuum tightness.
6. the analytical approach of microchip analysis system as claimed in claim 3 is characterized in that: sample introduction stage or the separation phase of controlling micro flow control chip capillary electrophoresis by the valve position of T-valve; The valve position of T-valve when with electrical means control control, is accurately controlled the time in sample introduction stage with electronic and manual two kinds of methods control with the time relay.
7. the analytical approach of microchip analysis system as claimed in claim 3, it is characterized in that: when chemical illuminating reagent is nonconducting non-aqueous solution, the non-aqueous solution of chemical illuminating reagent is flowed in a sheath circulation road therein, and add electrophoretic buffer, and the separation electric field of chip capillary cataphoresis is applied on the split tunnel by the sheath circulation road that electrophoretic buffer flows through at another sheath circulation road.
8. the analytical approach of microchip analysis system as claimed in claim 3 is characterized in that: when chemical illuminating reagent is the aqueous solution of conduction, the separation electric field of chip capillary cataphoresis is applied on the split tunnel by the sheath circulation road that chemical illuminating reagent flows through.
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| CN102243151A (en) * | 2010-09-20 | 2011-11-16 | 华东理工大学 | Zero dead volume capillary electrophoresis and laser induced fluorescence detection online derivatization device |
| CN102980996A (en) * | 2012-12-31 | 2013-03-20 | 广州市第一人民医院 | Chemiluminescence immunoassay system, as well as method and application thereof |
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| CN103868970A (en) * | 2014-03-21 | 2014-06-18 | 上海通微分析技术有限公司 | Full-automatic high-precision capillary electrophoresis apparatus |
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| CN102243151B (en) * | 2010-09-20 | 2014-04-23 | 华东理工大学 | Zero dead volume capillary electrophoresis and laser induced fluorescence detection online derivatization device |
| CN102243151A (en) * | 2010-09-20 | 2011-11-16 | 华东理工大学 | Zero dead volume capillary electrophoresis and laser induced fluorescence detection online derivatization device |
| CN102980996A (en) * | 2012-12-31 | 2013-03-20 | 广州市第一人民医院 | Chemiluminescence immunoassay system, as well as method and application thereof |
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| CN103868970B (en) * | 2014-03-21 | 2016-01-06 | 上海通微分析技术有限公司 | Full-automatic high precision capillary electrophoresis apparatus |
| CN103868970A (en) * | 2014-03-21 | 2014-06-18 | 上海通微分析技术有限公司 | Full-automatic high-precision capillary electrophoresis apparatus |
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