CN110118718A - Liquid fluid system, test method, the regulation method of negative pressure device and flow cytometer - Google Patents
Liquid fluid system, test method, the regulation method of negative pressure device and flow cytometer Download PDFInfo
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- CN110118718A CN110118718A CN201910450370.8A CN201910450370A CN110118718A CN 110118718 A CN110118718 A CN 110118718A CN 201910450370 A CN201910450370 A CN 201910450370A CN 110118718 A CN110118718 A CN 110118718A
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- 239000007788 liquid Substances 0.000 title claims abstract description 159
- 239000012530 fluid Substances 0.000 title claims abstract description 155
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000033228 biological regulation Effects 0.000 title claims abstract description 15
- 238000010998 test method Methods 0.000 title claims abstract description 10
- 238000005070 sampling Methods 0.000 claims abstract description 81
- 238000012544 monitoring process Methods 0.000 claims abstract description 33
- 239000002699 waste material Substances 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 26
- 230000000694 effects Effects 0.000 claims abstract description 5
- 238000007405 data analysis Methods 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 claims description 13
- 238000004458 analytical method Methods 0.000 claims description 11
- 230000008878 coupling Effects 0.000 claims description 9
- 238000010168 coupling process Methods 0.000 claims description 9
- 238000005859 coupling reaction Methods 0.000 claims description 9
- 230000003287 optical effect Effects 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 6
- 230000008054 signal transmission Effects 0.000 claims description 4
- 238000005086 pumping Methods 0.000 claims description 2
- 230000004044 response Effects 0.000 abstract description 7
- 230000006641 stabilisation Effects 0.000 abstract description 2
- 238000011105 stabilization Methods 0.000 abstract description 2
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000013022 venting Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
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- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
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Abstract
The present invention relates to a kind of liquid fluid system, test method, the regulation method of negative pressure device and flow cytometers.The liquid fluid system of the flow cytometer includes test device, sampling device, sheath liquid device and negative pressure device, and test device has flow chamber；Sampling device is connected to flow chamber；Sheath liquid device includes sheath liquid container, sheath liquid pump and sheath fluid monitoring part, and sheath liquid container is connected to flow chamber, and sheath liquid pump is set in the pipeline between sheath liquid container and flow chamber, and sheath fluid monitoring part is set between sheath liquid pump and flow chamber；Negative pressure device includes negative pressure pond, negative pressure pump, negative pressure monitoring part, negative pressure control valve and waste fluid container, negative pressure pond is connected to flow chamber, negative pressure pump connects negative pressure pond, and negative pressure monitoring part connects pressure value of the negative pressure pond to detect negative pressure pond, and negative pressure control valve is set between flow chamber and negative pressure pond；Waste fluid container is connected to negative pressure pond.Above-mentioned liquid fluid system can achieve the effect that system stabilization and fast response time by the way that independent negative pressure device is arranged.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of liquid fluid system, test method, negative pressure device
Regulation method and flow cytometer.
Background technique
Flow cytometer (Flow Cytometer) is glimmering by the scattering light and label of measurement cell and other biologic grains
Luminous intensity quickly analyzes particle physically or chemically, can measure multiple characteristic parameters from a cell simultaneously, carry out
Qualitative or quantitative analysis has the characteristics that speed is fast, precision is high, accuracy is good.Flow cytometer is mainly by liquid fluid system, optics
System, electronic system, four part of data analysis system composition.
Wherein, the effect of liquid fluid system (Fluidics System) is to form sample to be tested under the package of sheath fluid surely
Fixed sheath stream enables the cell in sample to flow successively through flow chamber detection zone and is detected.Currently, liquid fluid system is substantially all
It is divided into the parts such as sheath fluid, loading, test, waste liquid.Loaded fraction is responsible for for sample liquid being delivered in flow chamber, cooperates sheath fluid part
The sheath fluid of output forms stable sheath stream in flow chamber and is tested, and the waste liquid that waste liquid part is then responsible for flow out from flow chamber is arranged
Out to outside machine.Traditional streaming instrument liquid fluid system is divided into two classes: positive pressure loading system, negative pressure inhale sample system.Positive pressure loading system
Two classes can be divided into system again, one kind is syringe loading, is limited to the volume and precision of syringe；In addition a kind of is positive pressure pressure
Loading, mechanism is complicated, with high costs.Negative pressure inhales sample system and is generally divided into double pump negative pressure system and single pump two class of negative pressure system, double
It is complex to pump negative pressure system fluid path, cost is high；Although single pump negative pressure system fluid path is relatively easy, adjusted by throttling
Loading speed is saved, system is unstable, and response speed is slower.
Summary of the invention
Based on this, it is necessary to unstable for system existing for traditional single pump negative pressure system and response speed is slower asks
Topic provides a kind of system and stablizes, and the liquid fluid system of fast response time, test method, the regulation method of negative pressure device and streaming are thin
Born of the same parents' instrument.
Specific technical solution is as follows:
A kind of liquid fluid system of flow cytometer, including test device, the test device have flow chamber；
Sampling device, the sampling device are connected to the flow chamber, and the sampling device is used to carry out sample to be tested
It acquires and is delivered to the flow chamber；
Sheath liquid device, the sheath liquid device include sheath liquid container, sheath liquid pump and sheath fluid monitoring part, the sheath liquid container
It is connected to the flow chamber, the sheath liquid pump is set in the pipeline between the sheath liquid container and the flow chamber, the sheath fluid
Monitoring part is set in the pipeline between the sheath liquid pump and the flow chamber；The sheath liquid device is used to sheath fluid being delivered to institute
State flow chamber；And
Negative pressure device, the negative pressure device include negative pressure pond, negative pressure pump, negative pressure monitoring part, negative pressure control valve and give up
Liquid container, the negative pressure pond are connected to the flow chamber, and the negative pressure pump is connected to the negative pressure pond, the negative pressure monitoring part
Be connected to pressure value of the negative pressure pond to detect the negative pressure pond, the negative pressure control valve be set to the flow chamber with it is described
In pipeline between negative pressure pond, connection and closure of the negative pressure control valve to control the flow chamber Yu the negative pressure pond；
The waste fluid container is connected to the negative pressure pond, and the waste fluid container is used to collect the waste liquid of the negative pressure pond outflow.
The test device further includes first exhaust control valve in one of the embodiments, and the flow chamber has row
Gas channel, the first exhaust control valve can control the opening and closure of the exhaust passage.
The sampling device includes sampling needle, sample introduction needle and controlling of sampling valve in one of the embodiments, described to adopt
Sample needle is connected to the sample introduction needle, and the sample introduction needle is connected to the flow chamber, and the controlling of sampling valve is set to the sampling needle
In pipeline between the sample introduction needle, the controlling of sampling valve be used to control the connection of the sampling needle and the sample introduction needle with
Closure.
The sheath liquid device further includes sheath hydraulic control valve in one of the embodiments, and the sheath hydraulic control valve is set to institute
It states in the pipeline between sheath liquid container and the flow chamber, the sheath hydraulic control valve is for controlling the sheath liquid container and the stream
The connection and closure of dynamic room.
The sheath liquid device further includes filter and second exhaust control valve, the mistake in one of the embodiments,
Filter is located on the pipeline between the sheath liquid container and the flow chamber, and the second exhaust control valve can control the mistake
The opening and closure of the exhaust outlet of filter.
A kind of regulation method of the negative pressure device of the liquid fluid system of the flow cytometer, comprising the following steps:
The negative pressure pressure value in negative pressure pond maintains default negative pressure parameter value always, when the institute that negative pressure monitoring part monitors
When stating negative pressure pressure value and deviateing default negative pressure parameter value, the flow velocity and/or stream of fluid in the negative pressure pond are adjusted by negative pressure pump
To so that the negative pressure pressure value reaches the default negative pressure parameter value.
A kind of test method of the liquid fluid system of flow cytometer, also comprises the steps of:
It is fast before test to inhale sample, sample is placed under sampling needle, opens negative pressure control valve, controlling of sampling valve, sample is negative
The pipeline between fully sampled needle, sample introduction needle and sampling needle and the sample introduction needle can be flowed under pressure differential pressure action, be then turned on sheath fluid
Control valve, sheath liquid pump pumping sheath fluid enter flow chamber, and it is to be detected to be formed together sample flow with the sample for reaching flow chamber.
It also comprises the steps of: in one of the embodiments,
In test process, sheath hydraulic coupling is adjusted by control sheath liquid pump, by sheath fluid pressure monitor sensor sheath hydraulic coupling,
And then control speed of the sample by flow chamber；
At the end of test, controlling of sampling valve is first closed, continues to provide sheath fluid, indoor residual sample is flowed in cleaning, then
Close sheath hydraulic control valve and negative pressure control valve.
It also comprises the steps of: in one of the embodiments,
When cleaning sampling needle, sheath hydraulic control valve, controlling of sampling valve are opened, and sheath liquid pump pumps out sheath fluid and completes to sampling device
Cleaning.
A kind of flow cytometer, including optical system, electronic system and data analysis system further include the liquid stream
System, the optical system is for providing the sample flow that light beam irradiates in the flow chamber of the liquid fluid system, in the sample flow
Specific cell issue fluorescence through irradiation, the electronic system collects the fluorescence and the fluorescence signal is converted to telecommunications
Number, after by the electric signal transmission to the data analysis system, the data analysis system carries out data analysis.
Compared with prior art, the invention has the following advantages:
The liquid fluid system of above-mentioned flow cytometer can reach system stabilization and response by the way that independent negative pressure device is arranged
Fireballing effect.Specifically, negative pressure device includes negative pressure pond, negative pressure pump, negative pressure monitoring part and negative pressure control valve, is formed
One individual module can maintain negative pressure pond to be in stable always by slave computer (PLC Driver Card) direct regulation and control
Negative pressure state.When the liquid fluid system of above-mentioned flow cytometer need to be used to measure analysis, negative pressure control valve, loading are opened
Device can immediately enter measuring state, save the measurement waiting time.
In the liquid fluid system of above-mentioned flow cytometer, flow chamber is equipped with exhaust passage, and first exhaust control valve can control
The opening and closure of exhaust passage can realize that will flow indoor bubble directly excludes by opening first exhaust control valve,
The interference for flowing indoor bubble to analysis result is reduced, analysis precision is improved.
In the liquid fluid system of above-mentioned flow cytometer, it is equipped with and adopts in the pipeline between the sampling needle and sample introduction needle of sampling device
Sample control valve, user can cut off sample channel when needing to stop test in time, avoid sample backflow pollution sample, or
Person's sample crosses suction waste sample；Individually cleaning flow chamber can be realized by controlling the folding of controlling of sampling valve simultaneously, being not necessarily to will
Flow chamber cleans together with sampling needle, easy to operate, and can be avoided flow chamber building up inside and largely remain sample increase cleaning
Difficulty pollutes.
In the liquid fluid system of above-mentioned flow cytometer, sheath liquid device further includes filter and second exhaust control valve, mistake
Filter can be filtered sheath fluid, filter out impurity, improve sheath fluid purity, improve sample flow analytical precision.Second row gas control
The setting of valve processed can in time in discharge filter contained by sheath fluid bubble, avoid aeration from analyzing result.
The regulation method of the liquid fluid system of above-mentioned flow cytometer individually controls negative pressure pump by setting negative pressure parameter value
Revolving speed to make the pressure value in negative pressure pond it is stable maintain setting negative pressure parameter value.On this basis, flow cytometer
Liquid fluid system only needs regulation one parameter of sheath fluid pressure value i.e. and can control the flow velocity and linear velocity namely negative pressure device of sample flow
With the cooperation of sheath liquid device, so that the parameter that the liquid fluid system of flow cytometer integrally needs to control is less, liquid fluid system is whole
It runs more reliable.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the liquid fluid system of flow cytometer described in an embodiment.
Description of symbols
10, the liquid fluid system of flow cytometer；100, test device；110, flow chamber；111, injection port；112, sheath fluid
Mouthful；113, negative pressure mouth；120, first exhaust control valve；200, sampling device；210, sampling needle；220, sample introduction needle；230, it samples
Control valve；300, sheath liquid device；310, sheath liquid container；320, sheath liquid pump；330, sheath fluid monitoring part；340, sheath hydraulic control valve；
350, filter element；351, filter；352, second exhaust control valve；400, negative pressure device；410, negative pressure pond；420, negative pressure
Pump；430, negative pressure monitoring part；440, negative pressure control valve；500, waste fluid container.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.In attached drawing
Give presently preferred embodiments of the present invention.But the invention can be realized in many different forms, however it is not limited to this paper institute
The embodiment of description.On the contrary, purpose of providing these embodiments is keeps the understanding to the disclosure more thorough
Comprehensively.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
One embodiment of the invention is related to a kind of flow cytometer.Above-mentioned flow cytometer includes optical system, Department of Electronics
System, data analysis system, control system and liquid fluid system 10.
It is shown in Figure 1, the liquid fluid system 10 of above-mentioned flow cytometer, including test device 100, sampling device 200, sheath
Liquid device 300 and negative pressure device 400.
Test device 100 has flow chamber 110.
Sampling device 200 is connected to flow chamber 110, and sampling device 200 is for being acquired and being delivered to sample to be tested
Flow chamber 110.
Sheath liquid device 300, sheath liquid device 300 are connected to flow chamber 110, and sheath liquid device 300 is used to sheath fluid being delivered to stream
Dynamic room 110.
In a specific example, sheath liquid device 300 includes sheath liquid container 310, sheath liquid pump 320 and sheath fluid monitoring part
330.Sheath liquid container 310 is connected to by sheath fluid mouth 112 with flow chamber 110.Sheath liquid pump 320 is set to sheath liquid container 310 and flow chamber
In pipeline between 110.Sheath fluid monitoring part 330 is set in the pipeline between sheath liquid pump 320 and flow chamber 110.Sheath fluid monitoring
Component 330 can be pressure sensor.
Negative pressure device 400, negative pressure device 400 include negative pressure pond 410, negative pressure pump 420, negative pressure monitoring part 430 and bear
Pressure control valve 440.Negative pressure pond 410 is connected to flow chamber 110, and negative pressure pump 420 is connected to 410 negative pressure monitoring part 430 of negative pressure pond
It is connected to negative pressure pond 410 and is set to flow chamber 110 and negative pressure pond to detect the pressure value in negative pressure pond 410, negative pressure control valve 440
In pipeline between 410, connection and closure of the negative pressure control valve 440 to control flow chamber 110 Yu negative pressure pond 410.
Optical system is used to provide the sample flow in the flow chamber 110 of the liquid fluid system 10 of light beam irradiation flow cytometer,
Specific cell in sample flow issues fluorescence through irradiation, and electronic system collects fluorescence and fluorescence signal is converted to electric signal,
Afterwards by electric signal transmission to data analysis system, data analysis system carries out data analysis.
Control system includes the first control module and the second control module.First control module is electrically connected at negative pressure pump
420 and negative pressure monitoring part 430 with for individually control negative pressure device 400.Second control module is electrically connected at sheath liquid pump
320, sheath fluid monitoring part 330, optical system, electronic system and data transmission system.The setting of first control module can
Negative pressure device 100 is individually controlled, without carrying out data transmission between the second control module, is not take up the second control mould
The data transmission resources of block, so that the control response speed of negative pressure pump 420 is faster, the negative pressure pressure value in negative pressure pond 410 is more
Stablize.Wherein, the first control module is PLC control circuit, and the second control module includes flow cytometer master control borad, fluidic cell
Instrument control software and computer.
Specifically, flow chamber 110 has injection port 111, sheath fluid mouth 112 and negative pressure mouth 113.Sampling device 200 passes through
Injection port 111 is connected to flow chamber 110.Sheath liquid device 300 is connected to by sheath fluid mouth 112 with flow chamber 110.Negative pressure pond 410 is logical
Negative pressure mouth 113 is crossed to be connected to flow chamber 110.
Preferably, negative pressure pump 420 is the pump with anti-down fluid element.Alternatively, negative pressure device further includes backflow preventer.It is anti-down
Valve is flowed to be set in the pipeline between negative pressure pump 420 and negative pressure pond 410.Above-mentioned setting can prevent waste liquid from flowing back to negative pressure pond 410
In.Negative pressure monitoring part 430 is pressure sensor.
In a specific example, the liquid fluid system 10 of above-mentioned flow cytometer further includes waste fluid container 500.Waste fluid container
500 are connected to negative pressure pond 410, and waste fluid container 500 is used to collect the waste liquid of the outflow of negative pressure pond 410.The setting energy of waste fluid container 500
The waste liquid flowed out in negative pressure pond 410 is enough collected, waste liquid is avoided disorderly to splash influence environmental sanitation.
In a specific example, test device 100 further includes first exhaust control valve 120.Flow chamber 110 has exhaust
Channel, first exhaust control valve 120 can control the opening and closure of exhaust passage.Above-mentioned setting can be by opening first row
The realization of gas control valve 120 directly excludes the bubble in flow chamber 110, reduces the bubble in flow chamber 110 to analysis result
Interference improves analysis precision.
Preferably, first exhaust control valve 120 is venting plug.When that bubble need to be exhausted to flow chamber 110, it is only necessary to revolve
Loose first exhaust control valve 120, without all removing first exhaust control valve 120.It, can be quick after the completion of being vented bubble
First exhaust control valve 120 is screwed, gas is avoided to enter flow chamber 110 from exhaust passage.In addition, first exhaust control valve 120
It can reduce production cost for the setting of venting plug.
In a specific example, sampling device 200 includes sampling needle 210 and sample introduction needle 220.Sampling needle 210 with into
Sample needle 220 is connected to, and sample introduction needle 220 is connected to by injection port 111 with flow chamber 110.
Preferably, sampling device 200 further includes controlling of sampling valve 230.Controlling of sampling valve 230 be set to sampling needle 210 with into
In pipeline between sample needle 220, controlling of sampling valve 230 is used to control the connection and closure of sampling needle 210 Yu sample introduction needle 220.?
Controlling of sampling valve 230 is equipped in pipeline between the sampling needle 210 and sample introduction needle 220 of sampling device 200, user can pass through control
Individually cleaning flow chamber 110 is realized in the folding of controlling of sampling valve 230 processed, it is not necessary that flow chamber 110 and sampling needle 210 is clear together
It washes, it is easy to operate, and can be avoided 110 building up inside of flow chamber and largely remain sample increase cleaning difficulty.Controlling of sampling valve 230
Loading channel can be switched rapidly when stopping test, sample refluence pollution sample or sample is avoided to cross suction waste sample
This.
Preferably, sheath liquid device 300 further includes sheath hydraulic control valve 340.Sheath hydraulic control valve 340 be set to sheath liquid container 310 with
In pipeline between flow chamber 110, sheath hydraulic control valve 340 is used to control the connection of sheath liquid container 310 and flow chamber 110 and closes
It closes.The setting of sheath hydraulic control valve 340 can realize control liquid chamber 310 and flow chamber by controlling the folding of sheath hydraulic control valve 340
110 connection and closure.Before analysis starts, it is closed sheath hydraulic control valve 340, flow chamber 110 and sheath liquid device 300 are relatively solely
Vertical, the sheath fluid that can be avoided in sheath liquid device 300 flows into flow chamber 110.After analysis starts, sheath hydraulic control valve 340 is opened,
Flow chamber 110 is connected to sheath liquid device 300, and sheath fluid flows into flow chamber 110, and sample flow is collectively formed with sample to be tested.Sheath fluid
Control valve 340 can closing when starting test, avoid sheath fluid channel from accessing, lead to so that sample fill rapidly full loading
Road reaches the target of quick loading test.
It can be appreciated that controlling of sampling valve 230, sheath hydraulic control valve 340 and negative pressure control valve 440 can be realized control liquid
Road on-off, specifically, controlling of sampling valve 230, sheath hydraulic control valve 340 and negative pressure control valve 440 may each be plain radial valve
Or break valve.
In a specific example, sheath liquid device 300 further includes filter element 350.Filter element 350 is located at sheath liquid container
In pipeline between 310 and flow chamber 110.The setting of filter element 350 can be filtered sheath fluid in sheath liquid container 310,
The sheath fluid after impurity will be filtered out again to be delivered in flow chamber 110, effectively increases the purity of sheath fluid, and then improve sample flow analysis
Accuracy.
Further, filter element 350 includes filter 351 and second exhaust control valve 352.Filter 351 is located at
On pipeline between sheath liquid container 310 and flow chamber 110.Second exhaust control valve 352 is capable of the exhaust outlet of controlling filter
Open and closure.The bubble in filter element 350 contained by sheath fluid can be discharged in above-mentioned setting in time, and aeration is avoided to analyze
As a result.Second exhaust control valve 352 can be manual exhaust valve, it can be achieved that manual exhaust to filter, is effectively vented, and reduces
Cost.
Preferably, second exhaust control valve 352 is venting plug.When that bubble need to be exhausted to filter 351, it is only necessary to revolve
Loose second exhaust control valve 352, without all removing second exhaust control valve 352.It, can be quick after the completion of being vented bubble
Second exhaust control valve 352 is screwed, gas is avoided to enter filter 351 from exhaust outlet.In addition, second exhaust control valve 352 is
The setting of venting plug can reduce production cost.
The present embodiment additionally provides a kind of analysis method using above-mentioned flow cytometer, comprising the following steps:
Negative pressure pump 420 starts, and after negative pressure pond 410 forms stable negative pressure, opens negative pressure control valve 440, wink in flow chamber 110
Between generate negative pressure, open controlling of sampling valve 230, sample to be tested is followed by the sampling needle 210 of sampling device 200, controlling of sampling
Flow chamber 110 is flowed into after valve 230 and sample introduction needle 220.
Sheath hydraulic control valve 340 is opened, sheath liquid pump 320 works, and sheath fluid is flowed out by sheath liquid container 310, flows through filter element 350
And after sheath hydraulic control valve 340, flow chamber 110 is flowed into.Sample to be tested forms sample in flow chamber 110 under the package of sheath fluid
This stream.
Optical system provides the sample flow in light beam irradiation flow chamber 110, and sample flows through irradiation and generates fluorescence signal, electronics
After being converted to telecommunications after system collection fluorescence signal, and electric signal transmission to data analysis system is subjected to data analysis.
Sample flow after analyzing forms waste liquid, and waste liquid flows out to negative pressure pond 410 by flow chamber 110, then through negative pressure pump 420
It is expelled to waste fluid container 500.
Specifically, negative pressure pond 410 maintains stable negative pressure P1, P1 < -20kPa always.When test, sheath hydraulic coupling is set as
P2, P2 are determined jointly by the pipeline between the position and sheath fluid monitoring part 330 and sheath liquid container 310 of sheath fluid monitoring part 330
It is fixed.
The present embodiment additionally provides a kind of regulation side of the negative pressure device 400 of the liquid fluid system 10 of above-mentioned flow cytometer
Method, comprising the following steps:
The negative pressure pressure value in negative pressure pond 410 maintains default negative pressure parameter value always.When negative pressure monitoring part 430 monitors
When the negative pressure pressure value arrived is greater than default negative pressure parameter value P1, by negative pressure pump 420 adjust the flow velocity of fluid in negative pressure pond 410 with
So that negative pressure pressure value reduces until default negative pressure parameter value P1；When negative pressure pressure value is less than default negative pressure parameter value P1, lead to
It crosses negative pressure pump 420 and adjusts the flow velocity of fluid and/or flow direction in negative pressure pond 410 so that negative pressure pressure value increases until default negative pressure
Parameter value P1.So that negative pressure pressure value maintains stable default negative pressure parameter value P1 always.
Specifically, when negative pressure pressure value is greater than default negative pressure parameter value P1, the revolving speed of negative pressure pump 420 is turned up, works as negative pressure
When pressure value is less than default negative pressure parameter value P1, turns down the revolving speed of negative pressure pump 420 and/or adjust the steering of negative pressure pump 420.It is above-mentioned
The regulation method of the negative pressure device 400 of the liquid fluid system 10 of flow cytometer individually controls negative pressure by setting negative pressure parameter value
The revolving speed of pump 420 is to make stable the maintaining of the pressure value in negative pressure pond 410 set negative pressure parameter value.On this basis, streaming
The liquid fluid system 10 of cell instrument only needs the flow velocity and linear velocity of the i.e. controllable sample flow of regulation one parameter of sheath fluid pressure value.When
When sheath fluid monitoring part 330 monitors obtained sheath fluid pressure value greater than default sheath fluid pressure parameter value P2, pass through sheath liquid device 300
Sheath liquid pump 320 adjust the flow velocity of sheath fluid so that sheath fluid pressure value reduces until default sheath fluid pressure parameter value P2；Work as sheath fluid
When pressure value is less than default sheath fluid pressure parameter value P2, by the sheath liquid pump 320 of sheath liquid device 300 adjust sheath fluid flow velocity so that
Sheath fluid pressure value is obtained to increase until default sheath fluid pressure parameter value P2.Specifically, monitoring is obtained according to sheath fluid monitoring part 330
Sheath fluid pressure value and default sheath fluid pressure parameter value P2 calculate to obtain difference, sheath liquid pump 320 passes through PID calculation according to the difference
Method, which quickly calculates 320 revolving speed of sheath liquid pump, should increase or the amount of reduction, adapt to the fluctuation situation of sheath hydraulic coupling rapidly, directly
Reach stable to sheath hydraulic coupling.The cooperation of negative pressure device 400 and sheath liquid device 300, so that the liquid fluid system 10 of flow cytometer
The parameter for integrally needing to control is less, and liquid fluid system overall operation is more reliable.
Before the flow cytometer stated in use is analyzed, further include flow cytometer liquid fluid system 10 carry out just
Beginningization processing.The liquid fluid system 10 of above-mentioned flow cytometer carry out initialization process the following steps are included:
Manually second exhaust control valve 352 is unscrewed, then starts sheath liquid pump 320, filter 351 is filled.To
When having sheath fluid outflow at second exhaust channel, second exhaust control valve 352 is screwed.
Then manually first exhaust control valve first exhaust control valve 120 is unscrewed, opens sheath hydraulic control valve 340, sheath fluid
Flow chamber 110 is flowed into, when having sheath fluid outflow at exhaust passage, screws first exhaust control valve first exhaust control valve 120.
Controlling of sampling valve 230 is opened, sheath fluid charges sampling device 200, closes and adopts after having sheath fluid to flow out at needle 210 to be sampled
Sample control valve 230.
Negative pressure control valve 440 is opened, sheath fluid is filled into flow chamber 110, after sheath fluid fills flow chamber 110, closes negative pressure
Control valve 440 and sheath hydraulic control valve 340 close sheath liquid pump 320, complete initialization process.
The initialization process of the liquid fluid system 10 of above-mentioned flow cytometer, can be by the liquid fluid system 10 of flow cytometer
Present in a large amount of removal of bubbles.The bubble excluded in the liquid fluid system 10 of flow cytometer can be improved the steady of flow cytometer
It is qualitative, improve the accuracy of analysis result.
A kind of test method of the liquid fluid system 10 of flow cytometer, also comprises the steps of:
When tested, sample is placed under sampling needle 210, negative pressure control valve 440, controlling of sampling valve 230 are opened, and sample exists
Under biggish negative pressure differential pressure action, fully sampled needle 210, sample introduction needle 220 and sampling needle 210 and the sample introduction needle are filled rapidly
Pipeline between 220, and reach flow chamber 110；Sheath hydraulic control valve 340 is opened, sheath liquid pump 320 pumps sheath fluid and enters flow chamber
Sample in 110, with flow chamber 110 is formed together sample flow and is detected, and enters waste fluid container by negative pressure pond 410 after detection
500；
In test process, by controlling the rotational speed regulation sheath hydraulic coupling of sheath liquid pump 320, supervised by sheath fluid monitoring part 330
Sheath hydraulic coupling is controlled, and then controls speed of the sample by flow chamber；
When stopping test, controlling of sampling valve 230 is first closed, closes sheath hydraulic control valve after cleaning flow chamber 110 by sheath fluid
340 and negative pressure control valve 440；
When cleaning sampling needle 210, sheath hydraulic control valve 340, controlling of sampling valve 230 are opened, and sheath liquid pump 320 pumps out sheath fluid completion
Cleaning to sampling device 200.
When being cleaned to the liquid fluid system 10 of above-mentioned flow cytometer, comprising the following steps:
Sheath hydraulic control valve 340 is opened, starts sheath liquid pump 320 to provide sheath fluid, is then turned on negative pressure control valve 440, utilize
After sheath fluid washes the sample in flow chamber 110, negative pressure control valve 440 is closed.
Controlling of sampling valve 230 is opened, sheath fluid enters sampling needle 210 by sample introduction needle 220 and washes remaining sample, closes
Close controlling of sampling valve 230 and sheath hydraulic control valve 340, the i.e. cleaning of the liquid fluid system 10 of completion flow cytometer.
The method that the liquid fluid system 10 of above-mentioned flow cytometer is cleaned, it is simple to operate, save time power.
The liquid fluid system 10 of above-mentioned flow cytometer by be arranged independent negative pressure device 400 can reach system it is stable and
The effect of fast response time.Specifically, negative pressure device 400 include negative pressure pond 410, negative pressure pump 420, negative pressure monitoring part 430 with
And negative pressure control valve 440, an individual module is formd, negative pressure pond can be maintained by the direct regulation and control of PLC control circuit
410 are in stable negative pressure state always.When the liquid fluid system 10 of above-mentioned flow cytometer need to be used to measure analysis,
Negative pressure control valve 440 is opened, sampling device 400 can immediately enter measuring state, save the measurement waiting time.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of liquid fluid system of flow cytometer, which is characterized in that including test device, the test device has flowing
Room；
Sampling device, the sampling device are connected to the flow chamber, and the sampling device is for being acquired sample to be tested
And it is delivered to the flow chamber；
Sheath liquid device, the sheath liquid device include sheath liquid container, sheath liquid pump and sheath fluid monitoring part, the sheath liquid container and institute
Flow chamber connection is stated, the sheath liquid pump is set in the pipeline between the sheath liquid container and the flow chamber, the sheath fluid monitoring
Component is set in the pipeline between the sheath liquid pump and the flow chamber；The sheath liquid device is used to sheath fluid being delivered to the stream
Dynamic room；And
Negative pressure device, the negative pressure device include that negative pressure pond, negative pressure pump, negative pressure monitoring part, negative pressure control valve and waste liquid hold
Device, the negative pressure pond are connected to the flow chamber, and the negative pressure pump is connected to the negative pressure pond, the negative pressure monitoring part connection
In pressure value of the negative pressure pond to detect the negative pressure pond, the negative pressure control valve is set to the flow chamber and the negative pressure
In pipeline between pond, connection and closure of the negative pressure control valve to control the flow chamber Yu the negative pressure pond；It is described
Waste fluid container is connected to the negative pressure pond, and the waste fluid container is used to collect the waste liquid of the negative pressure pond outflow.
2. the liquid fluid system of flow cytometer according to claim 1, which is characterized in that the test device further includes
One gas exhausting valve, the flow chamber have exhaust passage, and the first exhaust control valve can control the exhaust passage
Open and closure.
3. the liquid fluid system of flow cytometer according to claim 1, which is characterized in that the sampling device includes sampling
Needle, sample introduction needle and controlling of sampling valve, the sampling needle are connected to the sample introduction needle, and the sample introduction needle and the flow chamber connect
Logical, the controlling of sampling valve is set in the pipeline between the sampling needle and the sample introduction needle, and the controlling of sampling valve is for controlling
Make the connection and closure of the sampling needle Yu the sample introduction needle.
4. the liquid fluid system of flow cytometer according to any one of claims 1 to 3, which is characterized in that the sheath fluid
Device further includes sheath hydraulic control valve, and the sheath hydraulic control valve is set in the pipeline between the sheath liquid container and the flow chamber,
The sheath hydraulic control valve is used to control the connection and closure of the sheath liquid container Yu the flow chamber.
5. the liquid fluid system of flow cytometer according to claim 4, which is characterized in that the sheath liquid device further included
Filter and second exhaust control valve, the filter are located on the pipeline between the sheath liquid container and the flow chamber, institute
The opening and closure of exhaust outlet of the filter can be controlled by stating second exhaust control valve.
6. a kind of regulation method of the negative pressure device of the liquid fluid system of flow cytometer described in Claims 1 to 5 any one,
Characterized by comprising the following steps:
The negative pressure pressure value in negative pressure pond maintains default negative pressure parameter value always, when negative pressure monitoring part monitor it is described negative
When pressure pressure value deviates default negative pressure parameter value, by negative pressure pump adjust in the negative pressure pond flow velocity of fluid and/or flow direction with
So that the negative pressure pressure value reaches the default negative pressure parameter value.
7. a kind of test method of the liquid fluid system of flow cytometer, which is characterized in that also comprise the steps of:
It is fast before test to inhale sample, sample is placed under sampling needle, opens negative pressure control valve, controlling of sampling valve, sample is in negative pressure pressure
The lower pipeline that can be flowed between fully sampled needle, sample introduction needle and sampling needle and the sample introduction needle of difference effect, is then turned on sheath fluid control
Valve, sheath liquid pump pumping sheath fluid enter flow chamber, and it is to be detected to be formed together sample flow with the sample for reaching flow chamber.
8. the test method of liquid fluid system according to claim 7, which is characterized in that also comprise the steps of:
In test process, sheath hydraulic coupling is adjusted by control sheath liquid pump, by sheath fluid pressure monitor sensor sheath hydraulic coupling, in turn
Control speed of the sample by flow chamber；
At the end of test, controlling of sampling valve is first closed, continues to provide sheath fluid, cleaning is flowed indoor residual sample, is then shut off
Sheath hydraulic control valve and negative pressure control valve.
9. the test method of liquid fluid system according to claim 8, which is characterized in that also comprise the steps of:
When cleaning sampling needle, sheath hydraulic control valve, controlling of sampling valve are opened, and sheath liquid pump pumps out sheath fluid and completes to the clear of sampling device
It washes.
10. a kind of flow cytometer, including optical system, electronic system and data analysis system, which is characterized in that further include
Liquid fluid system described in Claims 1 to 5 any one, the optical system irradiate the liquid fluid system for providing light beam
Sample flow in flow chamber, the specific cell in the sample flow issue fluorescence through irradiation, described in the electronic system is collected
The fluorescence signal is simultaneously converted to electric signal by fluorescence, after by the electric signal transmission to the data analysis system, the number
Data analysis is carried out according to analysis system.
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