CN107271630A - A kind of ballast hydroplankton on-line measuring device and method - Google Patents
A kind of ballast hydroplankton on-line measuring device and method Download PDFInfo
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- CN107271630A CN107271630A CN201710569993.8A CN201710569993A CN107271630A CN 107271630 A CN107271630 A CN 107271630A CN 201710569993 A CN201710569993 A CN 201710569993A CN 107271630 A CN107271630 A CN 107271630A
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- module
- ballast
- detection
- ballast water
- processing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
Abstract
The invention discloses a kind of ballast hydroplankton on-line measuring device and method, device includes:Filtering module, pre-processing module, detection module, digital sampling and processing, display module and control module;The filtering module includes being provided with the micro-valve of control piper break-make in the penstock being connected with ballast tank, the penstock, and part ballast water sample is admitted to example platform after filtering module and pre-processing module processing;Ballast water sample is after the detection module carries out fluoroscopic examination and the detection of impedance pulse voltage respectively, and obtained impedance pulse signal and fluorescence signal is collected by the signal acquisition process module, analyzed, and analysis result is delivered into display module;The control module is connected with the filtering module, detection module, digital sampling and processing and display module respectively, to each module action of control device.The present invention opens entire quantitatively control ballast water entrance by adjusting micro-valve, it is ensured that staff has the advantages that to monitor on-line and quantitatively detect to ballast water planktonic organism concentration real time inspection.
Description
Technical field
The present invention relates to ballast water for ship detection technique field, a kind of ballast hydroplankton is particularly related to online
Detection means and method.
Background technology
Ballast water for ship is one of main path of external marine biological invasion, and external harmful marine biotic intrusion is propagated,
Serious influence is all caused to ecological, industry, agricultural and human health etc..《Ballast water for ship and sediment monitoring and
Manage international convention》In the standard that should meet of ballast water performance when defining ship discharge, wherein one of re-detection i.e.
For the concentration of different size viable biologicals in ballast water.And planktonic organism value volume and range of product (most generally existing in ballast water for ship
One kind it is biological), be also the leading indicator of current ballast for cruising water process and detection.Numerous studies confirm that ballast water for ship is
The main path and carrier of invasive plants, the whole world exchange about 12,000,000,000 tons of ballast water every year, and survive in unprocessed pressure
Carry biological up to 7000 kinds in water, the potentially harmful marine organisms of ICES as shown by data 94% are passed by ballast water for ship
Broadcast.
To prevent the invasion of adventive in ballast water, International Maritime Organization (IMO) formulated in 2004《International shipping
Ballast water and its sediment monitoring and management contract》, the pact will in September in 2017 8 days it is formally effective.As the pact will
Come into force, China is used as maximum ballast water exporting country and importer, it is necessary to carry out correlative study work.But China is to sea at present
Lack further investigation in terms of the disaster monitoring that foreign adventive, particularly ballast water for ship introduce, it is impossible to meet portion of the Chinese government
Requirement of the door to ballast water adventive prevention and control.
With coming into force for compressive effect, related personnel needs the feelings of microorganism in ballast water in moment understanding ballast tank
Condition, and then adopt an effective measure, prevent because ballast water for ship index is unqualified, influence ship normal work.Currently for ship
The not systematic monitoring scheme of adventive and device in ballast water, the content concn for obtaining ballast hydroplankton compare
It is difficult, it is difficult to realize the implementation monitoring of planktonic organism, it is not up to standard to the discharge of ballast water when ship pulls in shore, cause economic loss
Situation happens occasionally.Therefore, the on-line monitoring problems demand of ballast water for ship is solved.
The content of the invention
In view of the deficiency that prior art is present, the invention aims to provide a kind of pressure that can constantly to any flow
Carry the device that water carries out planktonic organism on-line checking.
To achieve these goals, technical solution of the present invention is as follows:
A kind of ballast hydroplankton on-line measuring device, it is characterised in that device includes:Filtering module, pre-treatment
Module, detection module, digital sampling and processing, display module and control module;Wherein:
The filtering module includes being provided with control piper break-make in the penstock being connected with ballast tank, the penstock
Micro-valve, through filter resume module after part ballast water sample be admitted to pre-processing module;
The pre-processing module is pre-processed to the ballast water sample after filtering, and by pretreated ballast water sample
Send the detection module;The pretreatment at least includes separation, purification and dyed;
The detection module carries out fluoroscopic examination to pretreated ballast water sample respectively and impedance pulse voltage is detected
Afterwards, the impedance pulse signal and fluorescence signal obtained is collected by the digital sampling and processing, analyzed;
Analysis result is delivered to display module by the digital sampling and processing;
The control module connects with the filtering module, detection module, digital sampling and processing and display module respectively
Connect, to each module action of control device, and control module is additionally provided with the interface being connected with other equipment, for transmitting number
According to.
Further, as a preference of the present invention, the filtering module also include be arranged at water inlet the first filter membrane and
It is arranged at the second filter membrane of delivery port;The delivery port is connected to ballast tank by conduit, the unnecessary ballast water to send back to.
Further, as a preference of the present invention, the detection module includes the micro-fluidic core being arranged on example platform
Piece, fluoroscopic examination module and impedance pulse detection module;The fluoroscopic examination module includes light excitation component and light detection group
Part;The impedance pulse detection module includes impedance pulsed electrode and impedance pulse detection component.
Further, as a preference of the present invention, intaglio has what is be connected with filtering module successively on the micro-fluidic chip
Sample intake passage, sense channel and the sample output passage being connected with ballast tank, the sense channel both sides, provided with receiving impedance pulse
The groove of electrode.
Further, as a preference of the present invention, the fluoroscopic examination module is included to irradiate micro-fluidic chip detection
The light excitation component in region and the photodetection assembly for detecting microorganism fluorescence signal.
Another object of the present invention is to provide for a kind of ballast hydroplankton online test method based on said apparatus,
It is characterised in that it includes following steps:
1) device is installed, and device each several part is combined into connection successively and real by penstock and water output duct as requested
Now connected with ballast tank, realize that ballast water flows into filtering module by penstock, and unnecessary ballast water is by water output duct stream
Return ballast tank;
2) opening device, the entire further adjusting means ballast water of opening for adjusting micro-valve in penstock by control module enters
Water-carrying capacity, realizes that ballast water flows into filtering module by penstock, and unnecessary ballast water flows back to ballast tank by water output duct,
Separation, purification and the dying operation of pre-processing module are passed through by the ballast water sample of filtering module;Simultaneous opening device is each
Module, into working condition;
3) ballast water is detected, the ballast water sample partly handled through filtering module and pre-processing module flows into example platform,
Light excitation component irradiates micro-fluidic chip detection zone, and photodetection assembly gathers illuminated planktonic organism and produces fluorescence, and will
Fluorescence signal is sent to digital sampling and processing;Meanwhile, when planktonic organism passes through detection zone, produced between impedance pulsed electrode
Raw pulse voltage signal, impedance pulse detection component acquisition pulse voltage signal, and it is sent to data acquisition and control module;
4) data processing and result are shown, data acquisition and control module is to the fluorescence signal and impedance pulse voltage that receive
Signal is handled, and result is delivered to display module and shown, its display content at least includes the detection limit for detecting sample, inspection
Survey concentration and whether detection sample size is exceeded.
Compared with prior art, beneficial effects of the present invention:
1st, invention can be directly installed on ballast tank, and relevant staff can detect the life of swimming in ballast water in real time
Thing concentration, if the concentration measured is not inconsistent standardization, alarm can then occur for device.And then staff is to the ballast water in ballast tank
Situation has understanding directly perceived, meets the demand monitored in real time.
2nd, the present invention automatically controls micro-valve using microprocessor, sample is passed through detection zone according to the flow arbitrarily set
Domain, ballast water-carrying capacity is depending on the micro-valve opening time, so as to realize quantitative detection.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are the present invention
Some embodiments, for those of ordinary skill in the art, without having to pay creative labor, can be with root
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is apparatus of the present invention structural representation;
Fig. 2 is filtering module structural representation of the present invention;
Fig. 3 is inventive samples platform interior structural representation;
Fig. 4 is microfluidic chip structure schematic diagram of the present invention.
Drawing reference numeral explanation:
1st, ballast tank;2nd, micro-valve;3rd, filtering module;4th, example platform;5th, digital sampling and processing;6th, display module;
7th, control module 1;8th, conduit;9th, interface;10th, Ballast Management system;11st, pre-processing module;12nd, 10 μm of filter membranes;13、50μm
Filter membrane;14th, impedance pulse detection module;15th, fluoroscopic examination module;16th, light excitation component;17th, photodetection assembly;18th, miniflow
Control chip;19th, impedance pulsed electrode;20th, sample detection passage;21 detection zones;22nd, sample channel.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
A part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
Below in conjunction with the accompanying drawings and specific embodiment further illustrates technical scheme:
A kind of ballast hydroplankton on-line measuring device, it is characterised in that device includes:Filtering module 3, pre-treatment
Module 11, detection module, digital sampling and processing 5, display module 6 and control module 7;Wherein:
The filtering module 3 includes logical provided with control piper in the penstock being connected with ballast tank 1, the penstock
Disconnected micro-valve 2, the part ballast water sample after being handled through filtering module 3 is admitted to pre-processing module 11, in pre-processing module 11
Ballast water sample after filtering the operation such as separated to, purified, being dyed, then the ballast water sample after pre-treatment is sent to
In example platform 4.
Ballast water sample is after the detection module carries out fluoroscopic examination and the detection of impedance pulse voltage respectively, obtained resistance
Anti- pulse signal and fluorescence signal are collected by the signal acquisition process module, analyzed, and analysis result is delivered into display module
6;
The control module 7 respectively with the filtering module 3, detection module, digital sampling and processing 5 and display module
6 are connected, to each module action of control device, such as the control of micro-valve 2 ballast water flux magnitude, switch of detection means etc., are used for
Upload to interface of other equipment etc..
Exemplified by planktonic organism of the present embodiment by size in ballast water in 10~50 μ ms is monitored on-line.Device includes
Micro-valve 2, filtering module 3, pre-processing module 11, example platform 4, control module 7, digital sampling and processing 5 and display module 6
Composition.Micro-valve 2 is placed in conduit 8 and is connected with filtering module 3, filtering module 3 and example platform 4 by conduit 8 respectively with pressure
The two ends of cabin 1 are carried to be connected, and filtering module 3 is connected with example platform 4, digital sampling and processing 5 is connected with example platform 4
Connect, control module 7 is used for controlling the work of other modules.The micro-valve 2 is used to control the ballast water of firm discharge being flowed into
In device.
Further, as a preference of the present invention, the filtering module 3 also include be arranged at water inlet the first filter membrane and
It is arranged at the second filter membrane of delivery port;The delivery port is connected to ballast tank by conduit, the unnecessary ballast water to send back to.
Filtering module 3 is mainly made up of filter membrane in the present invention, and filter membrane includes 50 μm of filter membranes (the first filter membrane), 13 and 10 μm of filters
12 two kinds of film (the second filter membrane), its effect is 10~50 μm in ballast water for ship of planktonic organism is passed through filtering module.
Further, as a preference of the present invention, the detection module includes the micro-fluidic core being arranged on example platform
Piece 18, fluoroscopic examination module 15 and impedance pulse detection module 14;The fluoroscopic examination module 15 includes light excitation component 16
With photodetection assembly 17;The impedance pulse detection module 14 includes impedance pulsed electrode 19 and impedance pulse detection component.
Example platform 4 is main by micro-fluidic chip 18, fluoroscopic examination module 15, impedance pulse detection module 14 in the present invention
Composition, fluoroscopic examination module, impedance pulse detection module are used to detect the sample that detection zone 21 is flowed through in micro-fluidic chip.It is micro-
Fluidic chip material is to be provided with sense channel 22 inside polymethyl methacrylate (PMMA) or glass, the micro-fluidic chip,
Sense channel 22 is provided with detection zone 21, and fluoroscopic examination module 15 includes light excitation component 16 and photodetection assembly 17, and light is excited
Component 16 is radiated in detection zone 21 by exciting light, and planktonic organism is by exciting light irradiation to produce fluorescence at detection zone 21
Signal is simultaneously received by photodetection assembly 17, and impedance pulse detection module 14 is connected with impedance pulsed electrode 19, impedance pulsed electrode
19 are connected with the both sides of sense channel 22, for detecting change of the planktonic organism by voltage signal during sense channel 22.
Further, as a preference of the present invention, intaglio has and is connected with filtering module 3 successively on the micro-fluidic chip 18
Sample intake passage, sense channel 22 and the sample output passage being connected with ballast tank, the both sides of sense channel 22, provided with accommodate resistance
The groove of anti-pulsed electrode 19.
Further, as a preference of the present invention, the fluoroscopic examination module 15 is included to irradiate micro-fluidic chip inspection
Survey the light excitation component 16 in region and detect the photodetection assembly 17 of microorganism fluorescence signal.
Present invention offer is a kind of can to monitor the device of ballast water concentration in real time on ship, enable staff at any time
Whether detection ballast water concentration is up to standard in order to carrying out subsequent treatment.Device controls the flow of ballast water by micro-valve, utilizes filter
Film retains 10~50 μm of planktonic organism, then measures the dense of planktonic organism by impedance pulse detection and fluorescence detection method
Degree, data storage simultaneously includes measured result in display module, and the data of storage can be by interface to Ballast Management
System (BWMS) transmits data, and the present apparatus can control micro-valve to carry out circulating quantitative detection to ballast water by control module,
BWMS feedback result can be fed back to device so that device can be detected to the ballast water of any flow at any time.
The present invention also provides a kind of ballast hydroplankton online test method based on said apparatus, and its feature exists
In comprising the following steps:
1) device is installed, and device each several part is combined into connection successively and real by penstock and water output duct as requested
Now connected with ballast tank, realize that ballast water flows into filtering module by penstock, and unnecessary ballast water is by water output duct stream
Return ballast tank;
2) opening device, entire further adjusting means ballast water is opened by the micro-valve in control module regulation penstock
Flow of inlet water, realizes that ballast water flows into filtering module by penstock, and unnecessary ballast water flows back to ballast by water output duct
Cabin;Each module of simultaneous opening device, into working condition.
Specifically, when staff needs the planktonic organism in detection ballast tank in ballast water, control module can be passed through
Micro-valve is opened, control ballast water is quantitatively entered in detection means, flows subsequently through filtering module, filtering module front end has one
50 μm of filter membrane, the planktonic organism that can will be greater than 50 μm is blocked in outside filter membrane, and the filter membrane containing 10 μm is arranged at the bottom of filtering module,
By applying electric field so that sample leans on flows, the planktonic organism less than 10 μm is flowed out from bottom filter membrane and sends back to ballast tank
In.So by only 10~50 μm of planktonic organism of filtering module.Then, sample separated by pre-processing module,
The operations such as purification, dyeing, then the ballast water sample after pre-treatment is sent in example platform.
3) ballast water is detected, partly flows into example platform through filtering the ballast water sample of resume module, and light excitation component shines
Micro-fluidic chip detection zone is penetrated, photodetection assembly gathers illuminated planktonic organism and produces fluorescence, and fluorescence signal is sent
To digital sampling and processing;Meanwhile, when planktonic organism passes through detection zone, pulse voltage letter is produced between impedance pulsed electrode
Number, impedance pulse detection component acquisition pulse voltage signal, and it is sent to data acquisition and control module.
Specifically, sample is by the sense channel of example platform, passing through impedance pulse detection module and fluoroscopic examination mould
Block detects that the light excitation component in fluoroscopic examination module is irradiated to the detection zone in micro-fluidic chip so that the region internal pressure
Carry 10~50 μm of planktonic organisms in water to absorb exciting light and produce fluorescence, the fluorescence is detected by photodetection assembly, so that
Data processing module is sent to fluorescence signal, then by resulting fluorescence signal.Meanwhile, it is logical when there is planktonic organism to flow through detection
During road, the electrode detection at sense channel two ends is to voltage signal and to send a pulse signal to data processing module be impedance arteries and veins
Rush signal.Data processing module is handled resulting two kinds of signal, and the data handled well are shown in display module
And the firm discharge controlled by micro-valve before and the planktonic organism number that detects calculate the dense of 10~50 μm of planktonic organisms
Degree, in order to which staff can be intuitive to see the parameters such as sample signal and sample concentration.
4) data processing and result are shown, data acquisition and control module is to the fluorescence signal and impedance pulse voltage that receive
Signal is handled, and result is delivered to display module and shown, its display content includes detecting the detection limit of sample, detects dense
Whether degree and detection sample size are exceeded etc., control micro-valve time length to enter to ballast water sample flow by control module
Row is calculated, and in the case of known sample flow, can be calculated using the number of surveyed impedance pulse signal and fluorescence signal
All microalgae concentration and active microalgae concentration in sample.If the concentration over-standard measured, system prompt alarm so as to
Staff is further processed.
Specifically, measured signal can be stored, and the data of storage are uploaded into ballast pipe by interface
In reason system, further to be studied it.Then, the control module of device can according to staff's service condition or
The data that BWMS is fed back to control micro-valve to work again so that device can be detected to the ballast water of any flow at any time.
The present invention can detect ballast water in real time by the detection means being directly arranged on ballast tank according to use demand
In planktonic organism concentration, so that related personnel has one to get information about the ballast water situation in ballast tank, meet real
When the demand that monitors.The present apparatus automatically controls micro-valve using microprocessor, makes sample according to the flow arbitrarily set by detecting
Region, ballast water-carrying capacity is depending on the micro-valve opening time, so as to realize quantitative detection.
Finally it should be noted that:Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations;To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that:Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent substitution;And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (6)
1. a kind of ballast hydroplankton on-line measuring device, it is characterised in that device includes:Filtering module, pre-treatment mould
Block, detection module, digital sampling and processing, display module and control module;Wherein:
The filtering module includes micro- provided with control piper break-make in the penstock being connected with ballast tank, the penstock
Valve, the part ballast water sample after filtering resume module is admitted to pre-processing module;
The pre-processing module is pre-processed to the ballast water sample after filtering, and send institute by pretreated ballast water sample
State detection module;The pretreatment at least includes separation, purification and dyed;
The detection module is carried out to pretreated ballast water sample after fluoroscopic examination and the detection of impedance pulse voltage respectively, is obtained
The impedance pulse signal and fluorescence signal arrived is collected by the digital sampling and processing, analyzed;
Analysis result is delivered to display module by the digital sampling and processing;
The control module is connected with the filtering module, detection module, digital sampling and processing and display module respectively, is used
With each module action of control device, and control module is additionally provided with the interface being connected with other equipment, for transmitting data.
2. ballast hydroplankton on-line measuring device according to claim 1, it is characterised in that the filtering module
Also include being arranged at the first filter membrane of water inlet and be arranged at the second filter membrane of delivery port;The delivery port is connected to by conduit
Ballast tank, the unnecessary ballast water to send back to.
3. ballast hydroplankton on-line measuring device according to claim 1, it is characterised in that the detection module
Including the micro-fluidic chip being arranged on example platform, fluoroscopic examination module and impedance pulse detection module;The fluorescence inspection
Surveying module includes light excitation component and photodetection assembly;The impedance pulse detection module includes impedance pulsed electrode and impedance arteries and veins
Rush detection components.
4. ballast hydroplankton on-line measuring device according to claim 3, it is characterised in that the micro-fluidic core
Intaglio has the sample intake passage being connected with filtering module, sense channel and the sample output passage being connected with ballast tank, institute successively on piece
Sense channel both sides are stated, provided with the groove for accommodating impedance pulsed electrode.
5. ballast hydroplankton on-line measuring device according to claim 3, it is characterised in that the fluoroscopic examination
Module includes the light excitation component to irradiate micro-fluidic chip detection zone and detects the light detection of microorganism fluorescence signal
Component.
6. a kind of ballast hydroplankton online test method based on claim 1 described device, it is characterised in that including
Following steps:
1) device is installed, as requested by device each several part combine successively connection and by penstock and water output duct realize with
Ballast tank is connected, and realizes that ballast water flows into filtering module by penstock, and unnecessary ballast water flows back to pressure by water output duct
Carry cabin;
2) opening device, adjusts micro-valve in penstock by control module and opens entire further adjusting means ballast water feed water flow
Amount, realizes that ballast water flows into filtering module by penstock, and unnecessary ballast water flows back to ballast tank by water output duct, passes through
The ballast water sample of filtering module passes through separation, purification and the dying operation of pre-processing module;Each module of simultaneous opening device,
Into working condition;
3) ballast water is detected, the ballast water sample partly handled through filtering module and pre-processing module flows into example platform, and light swashs
Send out component irradiation micro-fluidic chip detection zone, photodetection assembly gathers illuminated planktonic organism and produces fluorescence, and by fluorescence
Signal is sent to digital sampling and processing;Meanwhile, when planktonic organism passes through detection zone, arteries and veins is produced between impedance pulsed electrode
Voltage signal, impedance pulse detection component acquisition pulse voltage signal are rushed, and is sent to data acquisition and control module;
4) data processing and result are shown, data acquisition and control module is to the fluorescence signal and impedance pulse voltage signal that receive
Handled, result is delivered to display module and shown, its display content at least includes the detection limit for detecting sample, detects dense
Whether degree and detection sample size are exceeded.
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CN201710569993.8A CN107271630A (en) | 2017-07-13 | 2017-07-13 | A kind of ballast hydroplankton on-line measuring device and method |
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CN114375822A (en) * | 2021-12-17 | 2022-04-22 | 江苏大学 | Nutrient solution return disinfection online monitoring system and method based on micro-fluidic chip |
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CN103616356A (en) * | 2013-11-25 | 2014-03-05 | 大连海事大学 | Device and method for classifying microalgae in ship ballast water |
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Application publication date: 20171020 |