CN110806491A - Full-automatic POCT multiple detection liquid phase chip system - Google Patents

Full-automatic POCT multiple detection liquid phase chip system Download PDF

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Publication number
CN110806491A
CN110806491A CN201911118125.3A CN201911118125A CN110806491A CN 110806491 A CN110806491 A CN 110806491A CN 201911118125 A CN201911118125 A CN 201911118125A CN 110806491 A CN110806491 A CN 110806491A
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guide rail
sample
liquid
full
magnetic separation
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CN110806491B (en
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张兴鹏
常乐
韦润聃
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Chengdu Yilexin Biotechnology Co Ltd
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Chengdu Yilexin Biotechnology Co Ltd
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Abstract

The utility model relates to a full-automatic chemiluminescence field specifically relates to a full-automatic POCT multiple detection liquid phase chip system, and it is including the mounting bracket, install application of sample arm, magnetic separation subassembly, light path system and main control board on the mounting bracket respectively, light path system and main control board are located the top of mounting bracket, light path system includes first laser instrument and the first reflection mirror holder that corresponds with it, second laser instrument and the second mirror holder that corresponds with it, the other mobile room that is provided with of second mirror holder, be provided with two cemented lens between mobile room and the second mirror holder, be provided with first lateral fluorescence around the mobile room and collect the subassembly, the subassembly is collected to the second lateral fluorescence and the subassembly is collected to forward scattered light. The optical path system adopts a modular design and can be flexibly configured according to the requirements of customers. The fluorescence collection system can adopt a single-chip aspheric focusing lens with large numerical aperture, and has simple and reliable structure and high stability.

Description

Full-automatic POCT multiple detection liquid phase chip system
Technical Field
The application relates to the field of full-automatic chemiluminescence, in particular to a full-automatic POCT multiple detection liquid phase chip system.
Background
Due to the characteristics of high automation degree, high sensitivity and the like, the large-scale chemiluminescence is widely applied in clinic, and is rapidly developed in recent years, so that the large-scale chemiluminescence becomes a mainstream methodology of immunoassay. However, each test can only detect one index, the detection flux is much lower than that of a biochemical analyzer, the mainstream speed measurement is generally 200T/H, the highest speed of the biochemical analyzer can reach 2000T/H, and the immunodetection index is far greater than that of the biochemical detection index from the viewpoint of detection items, so that the requirement on the high flux of immunity is a necessary trend, and for a single-index detection system, the detection flux is very difficult to improve because chemiluminescence relates to magnetic separation and cleaning, the step is the biggest bottleneck, and the detection flux is difficult to greatly improve. However, if the multi-index detection is carried out, the detection flux can be increased to 20 times of the original flux if 20 indexes can be detected at one time, and the speed can be higher than that of the existing biochemical analyzer, so that the multiple immunoassay is a necessary trend of clinical immunoassay development. At present, a main multi-index detection platform is a Luminex liquid phase chip system, and a full-automatic liquid phase chip system is provided based on the platform for scenic life, but the cost is high, the size is large, and the full-automatic liquid phase chip system is not suitable for middle and small hospitals. The existing POCT is basically based on the principle of chromatography, the detection sensitivity is not enough, the precision is not high, the chemiluminescence of the POCT is proposed by individual manufacturers, but the magnetic separation efficiency is not high, the automation degree is low, and the detection flux is small.
Disclosure of Invention
In order to solve the problems in the prior art, a full-automatic POCT multiple detection liquid-phase chip system capable of realizing multiple detection, supporting single-reagent packaging, small instrument volume, full automation, no need of manual sample adding, no carry pollution and high detection efficiency is provided.
In order to achieve the technical effects, the specific scheme of the application is as follows:
a full-automatic POCT multiple detection liquid phase chip system is characterized in that: the device comprises a mounting rack, wherein a sample adding arm, a sample pipe frame, a TIP box assembly, a constant temperature control bin, a TIP head unloading frame, a magnetic separation assembly, a light path system and a main control board are respectively mounted on the mounting rack;
light path system and master control board are located the top of mounting bracket, and light path system includes first laser instrument and the first mirror holder that corresponds with it, second laser instrument and the second mirror holder that corresponds with it, and the second mirror holder is other to be provided with the flow room, is provided with two cemented lens between flow room and the second mirror holder, is provided with first lateral fluorescence collection subassembly, second lateral fluorescence collection subassembly and forward scattered light collection subassembly around the flow room.
Further, the first laser is a 525nm laser, the second laser is a 638nm laser, or a 525nm laser; the first reflector frame and the second reflector frame are both a 45-degree reflector frame and two beam combiners; the double-cemented lens comprises a precise optical one-dimensional adjusting frame and a double-cemented lens.
Furthermore, the first lateral fluorescence collecting assembly and the second lateral fluorescence collecting assembly respectively comprise a single-chip aspheric focusing lens with an aperture larger than 0.5, a dichroic mirror, an optical filter and a detector; the forward scattered light collection assembly includes a lens, a filter, and a photodiode detector.
The sample liquid flow control device further comprises a liquid path system, wherein the liquid path system comprises two plunger pumps, an electromagnetic valve, a sheath liquid filter and a diaphragm pump, the two plunger pumps suck a sample into the flow chamber through pressure difference and simultaneously provide stable sheath liquid flow so that the sample flows through a detection area of the flow chamber under the wrapping of the sheath flow, the electromagnetic valve is used for controlling the on-off of liquid, the filter is used for filtering impurities in the sheath liquid, and the diaphragm pump is used for supplying cleaning liquid to the outer wall of the sample needle.
To the magnetism separable set, furtherly, first magnetism separable set is the same with second magnetism separable set structure, first magnetism separable set all includes main lead screw motor and the guide rail slider connecting block that links to each other with it with second magnetism separable set, guide rail slider connecting block cup joints on linear guide, guide rail slider connecting block is connected with bar magnet motor guide rail installing support, be fixed with bar magnet lead screw motor on the bar magnet motor guide rail installing support, be connected with bar magnet installing block on the bar magnet motor guide rail installing support, bar magnet installing block is connected with a plurality of bar magnets, bar magnet motor guide rail installing support bottom has the loading head that is used for loading disposable magnetism separation cover.
Aiming at the first magnetic separation assembly and the second magnetic separation assembly, further, a main screw rod motor is connected with a guide rail sliding block through a T-shaped screw rod nut; the linear guide rail is arranged on the motor guide rail mounting bracket, and the main screw rod motor is arranged on the motor guide rail mounting bracket.
Aiming at the first magnetic separation assembly and the second magnetic separation assembly, further, at least one optical axis guide rail is sleeved on the magnetic bar installation block, and a plurality of magnetic bars are connected to the bottom of the magnetic bar installation block; the bottom of the motor guide rail mounting bracket is provided with a reflection-type photoelectric detection switch for detecting whether the disposable magnetic separation sleeve is normally loaded or unloaded, and the reflection-type photoelectric detection switch is arranged beside the disposable magnetic separation sleeve.
To the application of sample arm, further, the application of sample arm includes X axle and Z axle, installs the plunger pump on the Z axle motor slide block of application of sample arm.
To sample pipe support, further, sample pipe support adopts crawler-type transmission structure.
For the TIP box assembly, further, the TIP box assembly moves in the Y-axis direction.
Aiming at the first reaction bin and the second reaction bin, further, the first reaction bin and the second reaction bin are two independent constant temperature control bins and can execute Y-axis direction movement.
Aiming at the TIP head unloading frame, further, the TIP head unloading frame is arranged above the constant temperature control bin, a waste bin is arranged right below the constant temperature control bin, and the waste bin is in a drawer type structure.
The flow type sample loading assembly is arranged above the flow type sample loading assembly, the light path support is connected with the light path system through a shockproof rubber seat, the flow type sample loading assembly is arranged above the two reaction bins, and the light path support is arranged below the light path support and executes movement in the X-axis direction and the Z-axis direction.
Furthermore, the instrument further comprises a control computer, an electrical control assembly and an external electrical interface, wherein the control computer is an industrial personal computer with a touch function, and is arranged on a front frame of the instrument shell. The electric control assembly comprises a switching power supply, a main control board, a motor drive board and a temperature control board, and the external electric interface comprises an alternating-current power supply three-in-one switch, a USB and a network interface.
The reagent strip matched with the system is provided with a handle, a two-dimensional code, a magnetic separation sleeve and a disposable membrane breaking head are arranged beside the handle, and a first diluent hole, a second diluent hole, a magnetic bead hole, a first reagent hole, a second reagent hole, a third reagent hole, a first cleaning liquid hole, a second cleaning liquid hole, a third cleaning liquid hole, a fourth cleaning liquid hole and a detection hole are arranged on the reagent strip side by side.
The application has the advantages that:
1. the optical path system adopts a modular design and can be flexibly configured according to the requirements of customers. The fluorescence collection system can adopt a single-chip aspheric focusing lens with large numerical aperture, and has simple and reliable structure and high stability; the detection of the side scattering light and the fluorescence adopts SiPM, the volume is small, the cost is low, the quantum efficiency is high, and the detection is even better than PMT at certain wavelength, so that higher signal-to-noise ratio can be obtained; meanwhile, due to the semiconductor process, the consistency between chips is far superior to that of PMT, the stability of the system is further improved, and the inter-platform difference between different instruments is reduced.
2. The liquid path system is driven by the plunger pump, so that the precision is high, the service life is long, and the maintenance cost is low; the sample is loaded by adopting a pressure difference mode, so that the dead volume is avoided, and the carrying pollution is less; specifically, two plunger pumps 2, one for supplying the sheath fluid and one for drawing the waste fluid from the outlet end of the flow chamber, can generate a stable negative pressure between the plunger pumps 2 at a speed higher than the sheath fluid supply speed, and by precisely controlling the speed of the plunger pumps, accurate sample suction can be achieved without any solenoid valve before the sample enters the flow chamber.
3. This application adopts mutually independent first reaction storehouse and second reaction storehouse, but both independent operation has realized the binary channels and has detected, can improve detection efficiency, compatible emergency call demand. This application adopts two kinds of active magnetic separation techniques of first magnetic separation subassembly and second magnetic separation subassembly, utilizes the cooperation of disposable magnetic separation cover and bar magnet, directly adsorbs the magnetic bead in the bottom of magnetic separation cover, reaches the effect of initiative magnetic separation, promotes the cleaning efficiency, has reduced the background of system.
4. The utility model provides a bar magnet motor drive magnetic separation cover among first magnetic separation subassembly and the second magnetic separation subassembly vibrates the mixing about lasting in the reaction process, has accelerated the immunoreaction process, has shortened reaction time, has avoided subsiding of magnetic bead, has realized whole mixing, has promoted reaction efficiency, has avoided the magnetic bead to subside, has improved the homogeneity of reaction.
5. The PMT reading device drives a rotating mechanism through a steering engine, a shutter and an attenuation sheet with the transmittance of 1% can be loaded on the rotating mechanism, and through switching, the luminous value before attenuation and the luminous value after attenuation can be respectively read, so that the dynamic range of detection is greatly improved, the reagent dosage is saved, and the detection efficiency is improved.
6. The sampling system adopts crawler-type to advance a kind, compares with traditional disc type sampling and has saved the space greatly, can support the sample simultaneously and insert at any time, better support emergency call demand. The disposable conductive TIP head can be adopted, liquid level detection and air pressure detection are equipped, full-automatic sample adding is realized, whole blood is supported, and no carrying pollution is caused. At present, POCT chemiluminescence of domestic manufacturers is manually added with sample, the steps are complicated, human errors are easily introduced, and the POCT chemiluminescence is not suitable for emergency treatment; the single-part reagent strip can be adopted, all consumables are packaged in the reagent strip, and the manual operation flow is simplified.
Drawings
Fig. 1 is a schematic structural diagram of the present application.
FIG. 2 is a schematic view of a matched reagent strip structure of the present application.
Fig. 3 is a schematic structural view of a magnetic separation assembly according to the present application.
Fig. 4 is a schematic diagram of the optical path structure of the present application.
Fig. 5 is a schematic view of a fluid path structure according to the present application.
In the drawings: 1-a sample adding arm, 2-a plunger pump, 3-a main control computer, 4-a sample tube rack, 5-a TIP box component, 6-a first reaction bin, 7-a second reaction bin, 8-a waste bin, 9-a TIP head unloading rack, 10-a reaction disc cable drag chain, 11-a first magnetic separation component, 12-a second magnetic separation component, 13-a light path bracket, 14-an external electrical interface, 15-a flow type sample adding component, 16-a light path system, 17-a main control board, 20-a handle, 21-a two-dimensional code, 22-a magnetic separation sleeve, 23-a disposable membrane breaking head, 24-a first diluent hole, 25-a second diluent hole, 26-a magnetic bead hole, 27-a first reagent hole, 28-a second reagent hole and 29-a third reagent hole, 30-first wash liquor hole, 31-second wash liquor hole, 32-third wash liquor hole, 33-fourth wash liquor hole, 34-detection hole.
101-main lead screw motor, 102-main motor guide rail mounting bracket, 103-T-shaped lead screw nut, 104-guide rail slider connecting block, 105-linear guide rail, 106-reflection type photoelectric detection switch, 107-disposable magnetic separation sleeve, 108-magnetic bar lead screw motor, 109-magnetic bar motor guide rail mounting bracket, 110-magnetic bar mounting block, 111-optical axis guide rail and 112-magnetic bar.
301-first laser, 302-second laser, 303-first mirror mount, 304-second mirror mount, 305-double cemented lens, 306-first lateral fluorescence collection assembly, 307-second lateral fluorescence collection assembly, 308-flow cell, 309-forward scattered light collection assembly.
201-electromagnetic valve, 202-sheath fluid filter, 203-first plunger pump, 204-diaphragm pump.
Detailed Description
Example 1
As shown in fig. 1, a full-automatic POCT multiple detection liquid-phase chip system includes a mounting rack, on which a sample-adding arm 1, a sample tube rack 4, a TIP box assembly 5, a thermostatic control chamber, a TIP head unloading frame 9, a magnetic separation assembly, an optical path system 16 and a main control board 17 are respectively mounted, the thermostatic control chamber includes a first reaction chamber 6 and a second reaction chamber 7, the magnetic separation assembly includes a first magnetic separation assembly 11 and a second magnetic separation assembly 12, the first magnetic separation assembly 11 is correspondingly disposed above the first reaction chamber 6, and the second magnetic separation assembly 12 is correspondingly disposed above the second reaction chamber 7;
as shown in fig. 4, the optical path system 16 and the main control board 17 are located on the top of the mounting rack, the optical path system 16 includes a first laser 301 and a first mirror frame 303 corresponding to the first laser, a second laser 302 and a second mirror frame 304 corresponding to the second laser, a flow chamber 308 is disposed beside the second mirror frame 304, a double cemented lens 305 is disposed between the flow chamber 308 and the second mirror frame 304, and a first lateral fluorescence collecting assembly 306, a second lateral fluorescence collecting assembly 307 and a forward scattered light collecting assembly are disposed around the flow chamber 308. When the microspheres in the sample pass through the detection area of the flow chamber 308 one by one, the microspheres are excited by the laser focused thereon to generate scattered light, coded fluorescence and fluorescence to be detected, the scattered light, the coded fluorescence and the fluorescence to be detected are respectively received by the scattered light detector and the fluorescence detector, and after the sampling by the high-speed ADC, the data is transmitted to the main control computer 3 for calculating a final result.
The optical path system 16 of the present application adopts a modular design, and can be flexibly configured according to the customer requirements. The fluorescence collection system can adopt a single-chip aspheric focusing lens with large numerical aperture, and has simple and reliable structure and high stability; the detection of the side scattering light and the fluorescence adopts SiPM, the volume is small, the cost is low, the quantum efficiency is high, and the detection is even better than PMT at certain wavelength, so that higher signal-to-noise ratio can be obtained; meanwhile, due to the semiconductor process, the consistency between chips is far superior to that of PMT, the stability of the system is further improved, and the inter-platform difference between different instruments is reduced.
Example 2
As shown in fig. 1, the full-automatic POCT multiple-detection liquid-phase chip system includes a mounting rack, on which a sample-adding arm 1, a sample tube rack 4, a TIP box assembly 5, a thermostatic control chamber, a TIP head unloading frame 9, a magnetic separation assembly, a light path system 16 and a main control board 17 are respectively mounted, the thermostatic control chamber includes a first reaction chamber 6 and a second reaction chamber 7, the magnetic separation assembly includes a first magnetic separation assembly 11 and a second magnetic separation assembly 12, the first magnetic separation assembly 11 is correspondingly disposed above the first reaction chamber 6, and the second magnetic separation assembly 12 is correspondingly disposed above the second reaction chamber 7;
the optical path system 16 and the main control board 17 are located on the top of the mounting rack, the optical path system 16 includes a first laser 301 and a first mirror frame 303 corresponding to the first laser, a second laser 302 and a second mirror frame 304 corresponding to the second laser, a flow chamber 308 is arranged beside the second mirror frame 304, a double cemented lens 305 is arranged between the flow chamber 308 and the second mirror frame 304, and a first lateral fluorescence collecting component 306, a second lateral fluorescence collecting component 307 and a forward scattered light collecting component are arranged around the flow chamber 308. When the microspheres in the sample pass through the detection area of the flow chamber 308 one by one, the microspheres are excited by the laser focused thereon to generate scattered light, coded fluorescence and fluorescence to be detected, the scattered light, the coded fluorescence and the fluorescence to be detected are respectively received by the scattered light detector and the fluorescence detector, and after the sampling by the high-speed ADC, the data is transmitted to the main control computer 3 for calculating a final result.
The first laser 301 is a 525nm laser, the second laser 302 is a 638nm laser, or one 525nm laser; the first reflector frame 303 and the second reflector frame 304 are both a 45-degree reflector frame and two beam combiners; the doublet 305 includes a precision optics one-dimensional stage and a piece of doublet 305. The first lateral fluorescence collecting component 306 and the second lateral fluorescence collecting component 307 respectively comprise a single-chip aspheric focusing lens with an aperture larger than 0.5, a dichroic mirror, an optical filter and a detector; the optical filter and the dichroic mirror can be flexibly configured according to the coding requirement of the fluorescent channel; the forward scattered light collection assembly includes a lens, a filter, and a photodiode detector.
In addition to the above structure, the system further includes a liquid path system, the liquid path system includes two plunger pumps 2, an electromagnetic valve 201, a sheath liquid filter 202 and a diaphragm pump 204, the two plunger pumps 2 suck the sample into the flow chamber 308 by the pressure difference, and at the same time, provide a stable sheath liquid flow, so that the sample flows through the detection area of the flow chamber 308 under the wrapping of the sheath flow, the electromagnetic valve 201 is used for controlling the on-off of the liquid, the filter is used for filtering impurities in the sheath liquid to avoid blocking the flow chamber 308, and the diaphragm pump 204 is used for supplying the sample needle outer wall cleaning liquid.
The first magnetic separation component 11 and the second magnetic separation component 12 are identical in structure, the first magnetic separation component 11 and the second magnetic separation component 12 respectively comprise a main lead screw motor 101 and a guide rail slider connecting block 104 connected with the main lead screw motor 101, the guide rail slider connecting block 104 is sleeved on a linear guide rail 105, the guide rail slider connecting block 104 is connected with a magnetic bar motor guide rail mounting support 109, a magnetic bar lead screw motor 108 is fixed on the magnetic bar motor guide rail mounting support 109, a magnetic bar mounting block 110 is connected on the magnetic bar motor guide rail mounting support 109, the magnetic bar mounting block 110 is connected with a plurality of magnetic bars 112, and a loading head used for loading the disposable magnetic separation sleeve 107 is arranged at the bottom of the magnetic bar motor guide rail mounting support 109. The loading head is loaded by friction and the disposable magnetic separation sleeve 107 can be loaded one or more. The first magnetic separation assembly 11 and the second magnetic separation assembly are respectively driven by a main screw motor 101 and a magnetic bar screw motor 108, and the two motors only move along the Z axis and are used for performing magnetic bead adsorption, magnetic bead transfer and magnetic bead mixing.
The magnetic separation component is in an active magnetic separation mode, a disposable magnetic separation sleeve 107 is adopted, magnetic beads are adsorbed to the outer wall of the disposable magnetic separation sleeve 107 from a solution through matching with a magnetic rod 112, then the magnetic beads are transferred to other reaction hole positions, the magnetic rod 112 is evacuated, the magnetic beads are resuspended through up-down oscillation of the disposable magnetic separation sleeve 107, the magnetic adsorption time is short, and only about 10-15S is needed. The traditional magnetic separation mode adopts passive magnetic separation, a magnetic field is arranged outside a reaction cup, after long-time adsorption, liquid is pumped out through a waste liquid pump, other solutions are added for re-suspension and uniform mixing, the adsorption efficiency is low, the adsorption time is long, and generally more than 45S; in addition, in the whole reaction process, the magnetic rod 112 arm can continuously vibrate up and down, on one hand, the avoided sedimentation of magnetic beads is prevented, the reaction is more sufficient, on the other hand, the continuous vibration accelerates the movement and contact probability of molecules, so that the whole immunoreaction is accelerated, and the reaction time can be shortened.
The main screw motor 101 is connected with the guide rail sliding block through a T-shaped screw nut 103; the linear guide 105 is located on a motor guide mounting bracket on which the main lead screw motor 101 is mounted.
The magnetic bar mounting block 110 is sleeved with at least one optical axis guide rail 111, and the bottom of the magnetic bar mounting block 110 is connected with a plurality of magnetic bars 112; the bottom of the motor guide rail mounting bracket is provided with a reflection-type photoelectric detection switch 106 for detecting whether the disposable magnetic separation sleeve 107 is normally loaded or unloaded, and the reflection-type photoelectric detection switch 106 is arranged beside the disposable magnetic separation sleeve.
Sample adding arm 1 includes X axle and Z axle for carry out X axle and Z axle's reciprocating motion, install plunger pump 2 on sample adding arm 1's the Z axle motor slide, plunger pump 2 cooperates sample adding arm 1 to accomplish TIP head and loads, functions such as liquid level detection, atmospheric pressure are surveyed, the sample is absorb, sample application, TIP head uninstallation.
The sample pipe frame 4 adopts a crawler-type transmission structure. The sample pipe support 4 is used for loading sample pipes and driving the sample pipes to rotate and transmit to the position where sample adding is needed, so that the volume of the instrument is greatly saved, and the sample can be inserted at any time.
The TIP cartridge assembly 5 moves in the Y-axis direction. The TIP box assembly 5 is used for loading TIP head consumables and driving the TIP box to run to a position where the TIP head needs to be loaded.
The first reaction bin 6 and the second reaction bin 7 are two independent constant temperature control bins and can both execute Y-axis direction movement. The disposable reagent strip can be loaded into 2 reaction bins respectively, is used for completing all immunoreactions, cleaning and reading after sample loading, and sequentially completes the processes of loading by a probe, membrane penetrating, sample loading, diluting, loading by a magnetic separation sleeve 22, magnetic bead multiple transfer, magnetic bead cleaning, magnetic bead transfer to substrate reading and the like according to the process of programming.
The TIP head unloading frame 9 is arranged above the constant temperature control bin, a waste bin 8 is arranged right below the constant temperature control bin, and the waste bin 8 is in a drawer type structure. The penetrating head, TIP head and magnetic separation sleeve 22 etc. are automatically unloaded and dropped to the waste bin 8, wherein the spent TIP head is automatically unloaded and dropped to the waste bin 8 by an unloading mechanism mounted on the TIP head unloading carriage 9.
Besides the structure, the system further comprises a flow type sample loading assembly 15 and an optical path support 13, wherein the optical path support 13 is located right above the flow type sample loading assembly 15 and used for bearing the whole optical path system 16, the optical path support 13 is connected with the optical path system 16 through a shockproof rubber seat and used for reducing the influence of external vibration on a detection result, the flow type sample loading assembly 15 is located above two reaction bins, and the movement in the X-axis direction and the Z-axis direction is executed below the optical path support 13 and used for detecting liquid to be detected in a reagent strip detection hole 34 which has completed reaction.
Besides the structure, the system also comprises a control computer, an electrical control assembly and an external electrical interface 14, wherein the control computer is an industrial personal computer with a touch function, and is arranged on a front frame of the instrument shell. The control computer is used for controlling the whole instrument and providing a man-machine interaction interface. The electrical control assembly comprises a switching power supply, a main control board 17, a motor drive board and a temperature control board, and the external electrical interface 14 comprises an alternating-current power supply three-in-one switch, a USB and a network interface. The external electrical interface 14 is used for connecting with commercial power, USB peripheral equipment, a network and the like; the electric control assembly is used for supplying power to the system and controlling the whole machine, the control system is communicated with a computer through a USB, and the main control board 17 is communicated with other control modules through a CAN bus.
Be provided with handle 20 on the reagent strip that matches with this system, handle 20 side is provided with two-dimensional code 21, magnetic separation cover 22 and disposable rupture of membranes head 23, is provided with first diluent hole 24, second diluent hole 25, magnetic bead hole 26, first reagent hole 27, second reagent hole 28, third reagent hole 29, first washing liquid hole 30, second washing liquid hole 31, third washing liquid hole 32, fourth washing liquid hole 33 and inspection hole 34 on the reagent strip side by side. Handle 20 is used for the user to get and puts the reagent strip, two-dimensional code 21 contains information such as reagent code and batch number, an automatic identification for the system, disposable magnetic separation cover 107 is used for carrying out the magnetic bead absorption, mixing and transfer, disposable broken membrane head 23 is used for wearing the aluminium membrane of every hole of broken reagent strip and seals, first diluent hole 24, second diluent hole 25 is used for carrying out the dilution of sample, magnetic bead hole 26 is used for the magnetic bead component of encapsulation reagent, first reagent hole 27, second reagent hole 28, third reagent hole 29 is used for the remaining reagent component of encapsulation, first cleaning solution hole 30, second cleaning solution hole 31, third cleaning solution hole 32, fourth cleaning solution hole 33 is used for carrying out the washing of magnetic bead, inspection hole 34 is used for resuspending the magnetic bead, and carry out the detection.
The optical path system 16 is of a modular design and can be flexibly configured according to the requirements of customers. The fluorescence collection system can adopt a single-chip aspheric focusing lens with large numerical aperture, and has simple and reliable structure and high stability; the detection of the side scattering light and the fluorescence adopts SiPM, the volume is small, the cost is low, the quantum efficiency is high, and the detection is even better than PMT at certain wavelength, so that higher signal-to-noise ratio can be obtained; meanwhile, due to the semiconductor process, the consistency between chips is far superior to that of PMT, the stability of the system is further improved, and the inter-platform difference between different instruments is reduced.
The liquid path system is driven by the plunger pump 2, so that the precision is high, the service life is long, and the maintenance cost is low; the sample is loaded by adopting a pressure difference mode, so that the dead volume is avoided, and the carrying pollution is less; specifically, two plunger pumps 2, one for supplying the sheath fluid and one for drawing the waste fluid from the outlet end of the flow chamber 308, may be used, and when the drawing speed is greater than the sheath fluid supply speed, a stable negative pressure may be generated between the 2 plunger pumps 2, and by precisely controlling the speed of the plunger pumps 2, accurate sample suction may be achieved, and the sample may not have any solenoid valve 201 before entering the flow chamber 308.
Mutually independent first reaction storehouse 6 and second reaction storehouse 7, but both independent operation has realized the binary channels and has detected, can improve detection efficiency, compatible emergency call demand. This application adopts two kinds of active magnetic separation techniques of first magnetic separation subassembly 11 and second magnetic separation subassembly 12, utilizes disposable magnetic separation cover 107 and bar magnet 112's cooperation, directly adsorbs the magnetic bead in the bottom of magnetic separation cover 22, reaches the effect of initiative magnetic separation, promotes the cleaning efficiency, has reduced the background of system.
The magnetic rod 112 motor drive magnetic separation sleeve 22 in the first magnetic separation component 11 and the second magnetic separation component 12 vibrates the mixing from top to bottom continuously in the reaction process, so that the immunoreaction process is accelerated, the reaction time is shortened, the sedimentation of magnetic beads is avoided, the whole-course mixing is realized, the reaction efficiency is improved, the sedimentation of magnetic beads is avoided, and the reaction uniformity is improved.
The PMT reading device drives a rotating mechanism through a steering engine, a shutter and an attenuation sheet with the transmittance of 1% can be loaded on the rotating mechanism, and through switching, the luminous value before attenuation and the luminous value after attenuation can be respectively read, so that the dynamic range of detection is greatly improved, the reagent dosage is saved, and the detection efficiency is improved.
The sampling system adopts crawler-type to advance a kind, compares with traditional disc type sampling and has saved the space greatly, can support the sample simultaneously and insert at any time, better support emergency call demand. The disposable conductive TIP head can be adopted, liquid level detection and air pressure detection are equipped, full-automatic sample adding is realized, whole blood is supported, and no carrying pollution is caused. At present, POCT chemiluminescence of domestic manufacturers is manually added with sample, the steps are complicated, human errors are easily introduced, and the POCT chemiluminescence is not suitable for emergency treatment; the single-part reagent strip can be adopted, all consumables are packaged in the reagent strip, and the manual operation flow is simplified.

Claims (9)

1. A full-automatic POCT multiple detection liquid phase chip system is characterized in that: the device comprises a mounting rack, wherein a sample adding arm (1), a sample pipe frame (4), a TIP box assembly (5), a constant temperature control bin, a TIP head unloading frame (9), a magnetic separation assembly, a light path system (16) and a main control board (17) are respectively mounted on the mounting rack, the constant temperature control bin comprises a first reaction bin (6) and a second reaction bin (7), the magnetic separation assembly comprises a first magnetic separation assembly (11) and a second magnetic separation assembly (12), the first magnetic separation assembly (11) is correspondingly arranged above the first reaction bin (6), and the second magnetic separation assembly (12) is correspondingly arranged above the second reaction bin (7);
light path system (16) and main control panel (17) are located the top of mounting bracket, light path system (16) include first laser instrument (301) and first mirror holder (303) that corresponds with it, second laser instrument (302) and second mirror holder (304) that correspond with it, second mirror holder (304) are other to be provided with flow chamber (308), be provided with between flow chamber (308) and second mirror holder (304) two cemented lens (305), be provided with first lateral fluorescence collection subassembly (306), second lateral fluorescence collection subassembly (307) and forward scattered light collection subassembly around flow chamber (308).
2. The full-automatic POCT multiple detection liquid-phase chip system according to claim 1, characterized in that: the first laser (301) is a 525nm laser, the second laser (302) is a 638nm laser, or a 525nm laser; the first reflector frame (303) and the second reflector frame (304) are both a 45-degree reflector frame and two beam combiners; the double cemented lens (305) comprises a precision optics one-dimensional adjusting bracket and a piece of double cemented lens (305).
3. The full-automatic POCT multiple-detection liquid-phase chip system according to claim 2, characterized in that: the first lateral fluorescence collection component (306) and the second lateral fluorescence collection component (307) respectively comprise a single-chip aspheric focusing lens with numerical aperture larger than 0.5, a dichroic mirror, an optical filter and a detector; the forward scattered light collection assembly includes a lens, a filter, and a photodiode detector.
4. The full-automatic POCT multiple detection liquid-phase chip system according to claim 1, characterized in that: the sample liquid sampling device is characterized by further comprising a liquid path system, wherein the liquid path system comprises two plunger pumps (2), an electromagnetic valve (201), a sheath liquid filter (202) and a diaphragm pump (204), the two plunger pumps (2) suck a sample into the flow chamber (308) through pressure difference and simultaneously provide stable sheath liquid flow, so that the sample flows through a detection area of the flow chamber (308) under the wrapping of the sheath flow, the electromagnetic valve (201) is used for controlling the on-off of the liquid, the filter is used for filtering impurities in the sheath liquid, and the diaphragm pump (204) is used for supplying a sample needle outer wall cleaning liquid.
5. The full-automatic POCT multiple detection liquid-phase chip system according to claim 1, characterized in that: first magnetism separable set (11) is the same with second magnetism separable set (12) structure, first magnetism separable set (11) all include main lead screw motor (101) and guide rail slider connecting block (104) that links to each other with it with second magnetism separable set (12), guide rail slider connecting block (104) cup joint on linear guide (105), guide rail slider connecting block (104) are connected with bar magnet motor guide rail installing support (109), be fixed with bar magnet lead screw motor (108) on bar magnet motor guide rail installing support (109), be connected with bar magnet installing block (110) on bar magnet motor guide rail installing support (109), bar magnet installing block (110) are connected with a plurality of bar magnets (112), bar magnet motor guide rail installing support (109) bottom is equipped with the loading head that is used for loading disposable magnetism separating sleeve (107).
6. The full-automatic POCT multiple detection liquid-phase chip system according to claim 5, wherein: the main screw rod motor (101) is connected with the guide rail sliding block through a T-shaped screw rod nut (103); the linear guide rail (105) is positioned on the motor guide rail mounting bracket, and the main lead screw motor (101) is mounted on the motor guide rail mounting bracket.
7. The full-automatic POCT multiple detection liquid-phase chip system according to claim 6, wherein: the magnetic bar mounting block (110) is sleeved with at least one optical axis guide rail (111), and the bottom of the magnetic bar mounting block (110) is connected with a plurality of magnetic bars (112); the bottom of the motor guide rail mounting bracket is provided with a reflection-type photoelectric detection switch (106) for detecting whether the disposable magnetic separation sleeve (107) is normally loaded or unloaded, and the reflection-type photoelectric detection switch (106) is arranged beside the disposable separation magnetic sleeve.
8. The full-automatic POCT multiple detection liquid-phase chip system according to claim 1, characterized in that: the sample adding arm (1) comprises an X axis and a Z axis, and a plunger pump (2) is arranged on a Z axis motor slide block of the sample adding arm (1); the sample pipe frame (4) adopts a crawler-type transmission structure; the TIP box assembly (5) moves along the Y-axis direction; the first reaction bin (6) and the second reaction bin (7) are two independent constant temperature control bins and can both execute Y-axis direction movement; the TIP head unloading frame (9) is arranged above the constant temperature control bin, a waste bin (8) is arranged right below the constant temperature control bin, and the waste bin (8) is in a drawer type structure.
9. The full-automatic POCT multiple detection liquid-phase chip system according to claim 1, characterized in that: the flow type sample loading device is characterized by further comprising a flow type sample loading assembly (15) and an optical path support (13), wherein the optical path support (13) is located right above the flow type sample loading assembly (15), the optical path support (13) is connected with an optical path system (16) through a shockproof rubber seat, the flow type sample loading assembly (15) is located above the two reaction bins, and the movement in the X-axis direction and the movement in the Z-axis direction are executed below the optical path support (13).
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