CN114047170A

CN114047170A - Constant temperature fluorescence detector and multichannel fluorescence detection structure

Info

Publication number: CN114047170A
Application number: CN202111426616.1A
Authority: CN
Inventors: 贺志民; 许明灶; 陈翀
Original assignee: Guangzhou Universal Junan Biotechnology Co ltd
Current assignee: Guangzhou Universal Junan Biotechnology Co ltd
Priority date: 2021-11-27
Filing date: 2021-11-27
Publication date: 2022-02-15
Anticipated expiration: 2041-11-27
Also published as: CN114047170B

Abstract

The invention relates to a constant-temperature fluorescence detector and a multi-channel fluorescence detection structure, wherein the multi-channel fluorescence detection structure comprises a reflector, a controller and a plurality of optical modules, and the optical modules are circumferentially arranged around a first axis and respectively emit exciting light to corresponding samples to be detected; the reflector is provided with a plurality of first reflecting surfaces and second reflecting surfaces which are arranged in one-to-one correspondence, the first reflecting surfaces are arranged circumferentially around the first axis, and the first reflecting surfaces reflect fluorescence generated by corresponding samples to be detected to the corresponding second reflecting surfaces; the second reflecting surface is used for reflecting the fluorescent light reflected by the corresponding first reflecting surface to the receiving part; the controller is electrically connected with the receiving piece and is used for detecting and analyzing the received fluorescence. The optical module emits exciting light to excite a sample to be detected to react to generate fluorescence, the fluorescence is reflected to the second reflecting surface through the first reflecting surface, the second reflecting surface reflects the fluorescence to a receiving part connected with the controller, static detection is achieved, the detection result is accurate, the size is reduced, and multiple or porous samples can be inspected.

Description

Constant temperature fluorescence detector and multichannel fluorescence detection structure

Technical Field

The invention relates to the technical field of medical instruments, in particular to a constant-temperature fluorescence detector and a multi-channel fluorescence detection structure.

Background

Biological reactions such as nucleic acid multiplex detection, chemiluminescence multiplex index or biochemical multiplex index and the like require multiplex detection and are generally realized by porous consumables such as eight-connected tubes, multi-channel fluorescence detection structure chambers or centrifugal microfluidic control disks; in order to meet the above requirements, the conventional detection apparatus adopts the following modes: 1. each hole/channel is provided with a set of independent optical systems, which is expensive and bulky; 2. the number of the branches 1 is more through optical fibers and the like, so that the scheme is large in size and relatively high in cost; 3. detecting in turn by rotating/moving the moving mechanical structure, etc.; however, in the fluorescent amplification instrument detection technology, the accuracy of a light path is an important part of the instrument, the existing light path collection is a single path, a sample to be detected is controlled to be turned to a specified position through motor rotation or an index plate and the like, then different samples are detected respectively, the detection result is easily influenced by the deviation of a rotation angle, and the rotation control involves motor control, a process assembly structure, transportation vibration deviation and the like, so that the light path is inaccurate.

Disclosure of Invention

Therefore, it is necessary to provide a constant-temperature fluorescence detector and a multi-channel fluorescence detection structure, which are not only accurate in detection but also small in size, for solving the problems of large size and inaccurate result of the traditional detection instrument.

A multi-channel fluorescence detection structure comprises

The optical modules are arranged circumferentially around the first axis and used for respectively emitting exciting light to corresponding samples to be detected;

the device comprises a reflector, a first light source and a second light source, wherein the reflector is provided with a plurality of first reflecting surfaces and second reflecting surfaces which are arranged in a one-to-one correspondence manner, and the first reflecting surfaces are circumferentially arranged around a first axis and used for reflecting fluorescence generated by a corresponding sample to be detected to the corresponding second reflecting surfaces; the second reflecting surface is used for reflecting the fluorescent light reflected by the corresponding first reflecting surface to the receiving part;

and the controller is electrically connected with the receiving piece and is used for detecting and analyzing the received fluorescence.

In one embodiment, the reflector is provided with a plurality of entry holes arranged at intervals around the first axis, one hole wall of each entry hole is the first reflecting surface, the other hole wall of each entry hole is the second reflecting surface, the second reflecting surfaces are located in a space surrounded by the first reflecting surfaces, and the second reflecting surfaces are connected to form a plurality of pyramid surfaces with the first axis as a center line.

In one embodiment, the multi-channel fluorescence detection structure further comprises a fixing seat, wherein a plurality of mounting holes and light emitting holes are formed in the fixing seat and circumferentially arranged around the first axis, the mounting holes and the light emitting holes are arranged in a one-to-one correspondence manner, the light emitting holes and the first reflecting surfaces are arranged in a corresponding manner, and the optical modules are arranged in the mounting holes in a one-to-one correspondence manner;

and/or the included angle between the mounting hole and the first axis is 30-60 degrees;

and/or the light outlet hole is positioned in a space surrounded by the mounting hole.

In one embodiment, the multi-channel fluorescence detection structure further comprises a lamp panel, the optical module comprises a laser lamp, and the laser lamp is mounted on or assembled on the lamp panel;

and/or the optical module further comprises a plano-convex lens, wherein the plano-convex lens is arranged in the mounting hole and is positioned on the light-emitting side of the laser lamp;

and/or the optical module further comprises a first optical filter, wherein the first optical filter is arranged in the mounting hole and is positioned on the light emergent side of the plano-convex lens;

and/or the optical module further comprises a gasket, wherein the gasket is arranged in the mounting hole and is positioned between the plano-convex lens and the first optical filter;

and/or, the optical module still includes the uide bushing, the uide bushing cover is established the laser lamp is close to the one end of lamp plate, the uide bushing is installed in the mounting hole.

In one embodiment, the multi-channel fluorescence detection structure further comprises a light shield, a plurality of via holes corresponding to the light-emitting holes one by one are formed in the light shield, second optical filters are correspondingly installed in the via holes, and the light shield is arranged in the light shield;

and/or the multi-channel fluorescence detection structure further comprises first shading cotton, first through holes corresponding to the second shading sheets one by one are formed in the first shading cotton, and the first shading cotton is located between the reflecting shade and the second optical filter;

and/or the multi-channel fluorescence detection structure further comprises second shading cotton, second through holes corresponding to the light emitting holes one by one are formed in the second shading cotton 8, through holes are formed in the lamp panel, the second shading cotton is located between the lamp panel and the light shield, and the second shading cotton covers the through holes;

and/or the multi-channel fluorescence detection structure further comprises third shading cotton, a third through hole corresponding to the second reflecting surface is formed in the third shading cotton, and the third shading cotton is located on one side, away from the optical module, of the reflecting cover;

and/or, the multichannel fluorescence detection structure still includes fourth shading cotton, be equipped with on the fourth shading cotton with receive the fourth through-hole that the piece corresponds, the fourth shading cotton be located the third shading cotton with receive between the piece.

The constant-temperature fluorescence detector comprises a shell, a heating module and a multi-channel fluorescence detection structure, wherein the heating module and the multi-channel fluorescence detection structure are arranged in the shell, and a mounting groove for mounting a micro-fluidic chip is formed between the heating module and the multi-channel fluorescence detection structure.

In one embodiment, the heating module comprises a heating part, a heat conducting part and a temperature control part, the heating part is attached to one side of the heat conducting part, the other side of the heat conducting part is provided with a yielding groove for accommodating a reaction cavity of the microfluidic chip, and the temperature control part is electrically connected with the heating part;

and/or, the heating module further comprises a temperature protector, and the temperature protector is electrically connected with the temperature control part.

In one embodiment, the heating module further includes a mounting seat, the heating member is fixed to the heat conducting member, the heat conducting member is fixed to the mounting seat, the mounting seat is connected to the fixing seat of the multi-channel fluorescence detection structure, and the lamp panel of the multi-channel fluorescence detection structure is fixed to the fixing seat through a supporting plate.

In one embodiment, the constant-temperature fluorescence detector further comprises a cover plate, the housing is provided with an insertion hole for inserting the microfluidic chip, and the cover plate is rotatably connected with the housing and used for opening or closing the insertion hole.

The shell is provided with a control switch and an indicator light, and the control switch and the indicator light are respectively electrically connected with the controller.

When the constant-temperature fluorescence detector and the multi-channel fluorescence detection structure are used, the optical module emits exciting light to a corresponding sample to be detected to excite the sample to be detected to react to generate fluorescence, each fluorescence firstly enters the first reflecting surface of the reflector and is reflected to the second reflecting surface by the first reflecting surface, the second reflecting surface finally reflects the fluorescence to a receiver connected with the controller, photoelectric signals are fed back in real time, test results are interpreted, can realize the inspection of multiple or porous samples, compared with the traditional mode that the sample to be detected needs to be rotated by a motor or an index plate, the detection process is static detection, the stability and consistency are better, the detection result is more accurate, and adopt the reflector to carry out the secondary reflection to each light path, realize the transmission of each light path, compare the tradition and establish independent optical system's mode through optic fibre reposition of redundant personnel or every passageway, effectively reduce whole volume.

Drawings

FIG. 1 is an exploded view of a multi-channel fluorescence detection architecture according to an embodiment of the present application;

FIG. 2 is a schematic diagram of optical path transmission of a multi-channel fluorescence detection structure according to an embodiment of the present application;

FIG. 3 is a schematic structural diagram of a reflector of a multi-channel fluorescence detection structure according to an embodiment of the present application;

FIG. 4 is a schematic view of a constant temperature fluorescence detector according to an embodiment of the present application;

FIG. 5 is a schematic view of the constant temperature fluorescent detector of FIG. 4 with the cover plate open;

FIG. 6 is a schematic diagram of the constant temperature fluorescence detector of FIG. 5 with the microfluidic chip not inserted;

FIG. 7 is another schematic diagram of the isothermal fluorescence detector of FIG. 5 with the microfluidic chip not inserted;

FIG. 8 is a schematic cross-sectional view of the isothermal fluorescence detector of FIG. 4;

fig. 9 is an electrical schematic diagram of a constant temperature fluorescence detector according to an embodiment of the present application.

Description of reference numerals:

1. a housing; 11. a control switch; 12. an indicator light; 13. an insertion hole; 14. a power supply interface; 15. a USB interface; 16. a power supply module; 2. a heating module; 21. a heat generating member; 22. a heat conductive member; 23. a temperature control member; 24. a temperature protector; 25. a mounting seat; 3. a cover plate; 4. a microfluidic chip; 41. a reaction chamber; 10. an optical module; 110. a laser light; 120. a plano-convex lens; 130. a first optical filter; 140. a gasket; 140. a guide sleeve; 20. a reflector; 210. a first boss portion; 212. a first reflective surface; 220. a second boss portion; 222. a second reflective surface; 230. entering a perforation hole; 240. an installation part; 250. a peripheral edge portion; 260. a partition plate; 270. a positioning member; 30. a controller; 310. a receiver; 40. a fixed seat; 410. mounting holes; 420. a light exit hole; 50. a lamp panel; 510. a through hole; 55. a support plate; 60. a light shield; 610. a second optical filter; 620. a via hole; 710. first shading cotton; 712. a first through hole; 720. second shading cotton; 722. a second through hole; 730. third shading cotton; 732. a third through hole; 740. fourth shading cotton; 742. and a fourth via.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1, 4 and 8, a constant temperature fluorescence detector provided in an embodiment of the present invention includes a housing 1, a heating module 2 and a multi-channel fluorescence detection structure. The heating module 2 and the multi-channel fluorescence detection structure are arranged in the shell 1. And an installation groove for installing a microfluidic chip 4 is formed between the heating module 2 and the multi-channel fluorescence detection structure. The micro-fluidic chip 4 injected with the sample to be detected is inserted into the mounting groove, the reaction cavity 41 of the micro-fluidic chip 4 is heated at controlled temperature through the heating module 2, the light is emitted into the reaction cavity 41 through the multi-channel fluorescence detection structure, the sample to be detected in the reaction cavity 41 is excited to react to generate fluorescence, and the fluorescence is received for detection and analysis.

Referring to fig. 1 and 8, in one embodiment, the multi-channel fluorescence detection structure includes a reflector 20, a controller 30, and a plurality of optical modules 10. The optical modules 10 are circumferentially arranged around the first axis a and are used for respectively emitting exciting light to corresponding samples to be detected. Referring to fig. 3, the reflector 20 is provided with a plurality of first reflecting surfaces 212 and second reflecting surfaces 222 which are arranged in a one-to-one correspondence manner, and the first reflecting surfaces 212 are circumferentially arranged around the first axis and used for reflecting fluorescence generated by a corresponding sample to be detected to the corresponding second reflecting surfaces 222. The second reflection surface 222 is used for reflecting the fluorescent light reflected by the corresponding first reflection surface 212 to the receiving element 310. The controller 30 is electrically connected to the receiving element 310, and is used for detecting and analyzing the received fluorescence. The micro-fluidic chip 4 used in cooperation has a plurality of reaction chambers 41, and each reaction chamber 41 corresponds to each optical module 10.

When in use, the optical module 10 emits exciting light to a corresponding sample to be tested to excite the sample to be tested to react to generate fluorescence, the fluorescence firstly enters the first reflecting surface 212 of the reflector 20 and is reflected to the second reflecting surface 222 by the first reflecting surface 212, the second reflecting surface 222 finally reflects the fluorescence to the receiving part 310 connected with the controller 30, the photoelectric signal is fed back in real time to interpret the test result, can realize the inspection of multiple or porous samples, compared with the traditional mode that the sample to be detected needs to be rotated by a motor or an index plate and the like, this testing process is static detection, and stability and uniformity are better, and the testing result is more accurate, adopts reflector 20 to carry out the secondary reflection to each light path moreover, realizes the transmission of each light path, compares the tradition and establishes independent optical system's mode through optic fibre reposition of redundant personnel or every passageway, effectively reduces whole volume, saves the part, and the cost is lower.

Based on the principle of constant temperature reaction, the optical module 10 converges the excitation light on the sample to be detected, and the reflector 20 transmits the emitted light (light intensity change caused by fluorescence or absorbance) generated by the reaction in the sample to be detected, and the emitted light is imaged on the receiving part 310 of CCD, CMOS, PMT, photocell, photodiode or other photoelectric conversion to generate analog signals or digital signals and data files, thereby facilitating the dynamic monitoring and real-time light intensity change signal analysis of the nucleic acid constant temperature amplification process. On the basis of the multi-channel fluorescence detection structure, a multi-channel multiple inspection technology is adopted, and a static multi-channel one-receiving display or result reading detection is adopted, so that the detection result is accurate, and the misjudgment of false yin and yang results is solved and avoided.

Referring to fig. 2 and 3, in one embodiment, the reflector has a plurality of entry holes 230 disposed around the first axis at intervals, one hole wall of the entry holes 230 is the first reflecting surface 212, and the other hole wall is the second reflecting surface 222. The light path is transmitted to the first reflective surface 212 through the incident hole 230 and then reflected to the second reflective surface 222. The first reflective surface 212 faces the side of the sample to be tested and the second reflective surface 222 faces the side of the receiving element 310.

Referring to fig. 3 and 8, the second reflecting surface 222 is further located in a space surrounded by the first reflecting surface 212. The second reflecting surfaces 222 are connected to form a multi-pyramid surface having the first axis as a center line. This embodiment shows an eight-pyramid surface with eight second reflecting surfaces 222 and 8 first reflecting surfaces 212, respectively, which can be used with the microfluidic chip 4 with 8 and less than 8 reaction chambers 41. Referring to fig. 7, in the present embodiment, 7 reaction cavities 41 are disposed on the microfluidic chip 4, and 7 optical modules 10 are correspondingly disposed, where the 7 reaction cavities 41 respectively and correspondingly excite a reaction by light emitted from one optical module 10, and the generated fluorescence is respectively and correspondingly emitted to one first reflection surface 212. In other embodiments, 8, 6, 5, 4, 3, or 2 reaction cavities 41 may be provided on the

microfluidic chip

4, and 8, 6, 5, 4, 3, or 2 optical modules 10 are correspondingly provided, so that the light path arrangement transmission to the receiving element 310 can also be realized by the reflector 20 having eight second reflecting surfaces 222 and 8 first reflecting surfaces 212. According to practical requirements, the reflecting cover 20 may further include another number of second reflecting surfaces 222 and first reflecting surfaces 212, for example, 6 or 9, etc., so as to satisfy the detection of the microfluidic chip 4 having less than 6 or less than 9 equal numbers of reaction chambers 41.

Referring to fig. 8, the reflector 20 further includes a first protrusion 210 and a second protrusion 220. The first protrusion 210 has a plurality of first reflection surfaces 212. The second protrusion 220 has a plurality of second reflection surfaces 222. The plurality of first reflective surfaces 212 are connected to define a receiving hole. The second protruding portion 220 is disposed in the receiving hole and has a gap with the first protruding portion 210. The adjacent first reflecting surfaces 212 are separated by a partition 260, and one end of the partition 260 is connected to the first protruding portion 210 and the other end is connected to the second protruding portion 220. The plurality of spacers 260 partition the gap between the first and

second protrusions

210 and 220 into a plurality of the entry holes 230.

Further, the body further includes a mounting portion 240, the mounting portion 240 is disposed around the periphery of the first protruding portion 210, and a positioning member 270 is disposed on the mounting portion 240, so that the reflector 20 can be quickly positioned and mounted by the positioning member 270, and the first reflecting surface 212 can correspond to each optical path. The mounting portion 240 has a first surface facing the optical module 10 and a second surface facing away from the optical module 10. The first protrusion 210 protrudes toward the side of the second surface opposite to the first surface, and the second protrusion 220 protrudes toward the side of the second surface opposite to the first surface. The first reflecting surface 212 is disposed obliquely to the first surface. The second reflecting surface 222 is disposed obliquely to the first surface. Referring to fig. 3, in an embodiment, the inclination of the first reflecting surface 212 is smaller than that of the second reflecting surface 222, i.e. the included angle between the second reflecting surface 222 and the first axis is larger than that between the first reflecting surface 212 and the first axis. Optionally, the second reflecting surface 222 forms an angle of 60 ° with the first axis; the first reflective surface 212 is angled at 45 deg. to the first axis. In other embodiments, the included angle between the second reflecting surface 222 and the first reflecting surface 212 and the first axis may be other angles, so that the light path is transmitted to the receiving element for receiving through two reflections.

Further, the reflector 20 is an integrally formed structure. The controller 30 patrols and examines a plurality of samples, then each light path carries out secondary reflection through the reflector 20 that has a plurality of facets and gathers to receiving piece 310, feeds back photoelectric signal in real time, then interprets the test result through control software, realizes static detection, fully solves the instability that brings because motion conversion detects. The reflector 20 adopts a special mirror surface process as an integral component, has stability and uniformity, and controls the fluorescence generated by the reaction within a range.

Referring to fig. 1 and 8, in an embodiment, the multichannel fluorescence detection structure further includes a fixing base 40, where the fixing base 40 is provided with a plurality of mounting holes 410 and light emitting holes 420 circumferentially arranged around the first axis, and the mounting holes 410 and the light emitting holes 420 are arranged in a one-to-one correspondence; the optical modules 10 are disposed in the mounting holes 410 in a one-to-one correspondence. Each optical module 10 is mounted in a corresponding mounting hole 410. The light emitting holes 420 are disposed in one-to-one correspondence with the first reflective surfaces 212. In this embodiment, 7 mounting holes 410 are provided, 7 optical modules 10 are correspondingly provided, and 7 light-emitting holes 420 are correspondingly provided; the 7 light emitting holes 420 correspond to 7 of the first reflecting surfaces 212 one to one.

Further, the angle between the mounting hole 410 and the first axis is 30-60 °. In order to reduce the influence of background on the reagent or sample fluorescent substance, the included angle between the mounting hole 410 and the first axis is set to be 30-60 degrees, so that the exciting light emitted by the optical module 10 excites the fluorescent substance at the incident angle of 30-60 degrees, and the accuracy of the detection result is improved.

Further, the light emitting hole 420 is located in a space surrounded by the mounting hole 410. The layout of each part is more compact, and the whole volume is further reduced.

In an embodiment, the multi-channel fluorescence detection structure further includes a lamp panel 50, the optical module 10 includes a laser lamp 110, and the laser lamp 110 is installed on or assembled to the lamp panel 50. The lamp panel 50 is fixed to the fixing base 40 through a supporting plate 55. The laser lamp 110 is an LED lamp, and provides excitation light for the sample to be tested. Lamp plate 50 and controller 30 electric connection, laser lamp 110 is connected with lamp plate 50, realizes the control to each laser lamp 110 to polish each sample that awaits measuring, realize patrolling and examining to the static state of a plurality of samples.

Further, the optical module 10 further includes a plano-convex lens 120, and the plano-convex lens 120 is installed in the installation hole 410 and located on the light emitting side of the laser lamp 110. The light source of the laser lamp 110 is condensed into parallel light by the plano-convex lens 120.

Further, the optical module 10 further includes a first optical filter 130, and the first optical filter 130 is installed in the installation hole 410 and located on the light-emitting side of the plano-convex lens 120. The first filter 130 is used to filter the excitation light transmitted by the planoconvex lens 120 to a used waveband, and the filtered excitation light is emitted to the sample to be measured.

Further, the optical module 10 further includes a gasket 140, and the gasket 140 is installed in the installation hole 410 and located between the plano-convex lens 120 and the first optical filter 130. The gasket 140 serves to support the first filter 130 and the plano-convex lens 120.

Further, optical module 10 still includes uide bushing 140, uide bushing 140 cover is established the one end that laser lamp 110 is close to lamp plate 50, uide bushing 140 is installed in mounting hole 410. The guide sleeve 140 is used to fix and center the laser lamp 110 so that it is positioned and fixed in the mounting hole 410.

In an embodiment, the multi-channel fluorescence detection structure further includes a light shield 60, a plurality of via holes 620 corresponding to the light emitting holes 420 in a one-to-one manner are formed in the light shield 60, second optical filters 610 are correspondingly installed in the via holes 620, and the light reflector 20 is disposed in the light shield 60. The light shield 60 is used to fix the second filter 610 in the via hole 620. The second filter 610 serves to filter the light transmitted through the light exit hole 420 to a wavelength band used. The reflector 20 is disposed in the light shield 60, so that the reflector 20 is limited to the light shield 60. Further, a positioning column is further arranged in the light shield 60, and the positioning column is matched with the positioning piece 270 of the light reflecting cover 20, so that the position of the light reflecting cover 20 is positioned, and each light path can be accurately transmitted to the receiving piece 310.

Further, the multi-channel fluorescence detection structure further includes a first light shielding cotton 710, the first light shielding cotton 710 is provided with first through holes 712 corresponding to the second light shielding sheets one to one, and the first light shielding cotton 710 is located between the reflector 20 and the second optical filter 610. The second filter 610 is pressed and fixed by the first light shielding cotton 710, and then the reflector 20 is placed in the light shielding cover 60 and the first light shielding cotton 710 is pressed, so that the alignment accuracy of each element is ensured. The influence of light entering from the side surface is prevented after the first shading cotton 710 is fixed in the front and back direction, and the accuracy of a detection result is improved.

Further, the multichannel fluorescence detection structure further includes second light-shielding cotton 720, second through holes 722 corresponding to the light-emitting holes 420 in a one-to-one manner are formed in the second light-shielding cotton 720, a through hole 510 is formed in the lamp panel 50, the second light-shielding cotton 720 is located between the lamp panel 50 and the light-shielding cover 60, and the second light-shielding cotton 720 is covered and arranged at the through hole 510. The influence of light entering from the side surface is prevented after the second shading cotton 720 is fixed in the front and back direction, and the accuracy of the detection result is further improved.

Further, the multi-channel fluorescence detection structure further includes third light shielding cotton 730, a third through hole 732 corresponding to the second reflection surface 222 is disposed on the third light shielding cotton 730, and the third light shielding cotton 730 is located on a side of the reflector away from the optical module 10. The influence of light entering from the side surface is prevented after the third shading cotton 730 is fixed back and forth, and the accuracy of the detection result is further improved.

Referring to fig. 1 and 3, the reflector 20 further includes a peripheral edge portion 250, the peripheral edge portion 250 is disposed around the mounting portion 240, and the peripheral edge portion 250 extends toward a side of the second surface opposite to the first surface. On the one hand, the light-shading cotton plays a role of shading light, and on the other hand, the light-shading cotton is used for supporting the third light-shading cotton 730.

Further, the multi-channel fluorescence detection structure further includes a fourth light shielding cotton 740, a fourth through hole 742 corresponding to the receiving element 310 is provided on the fourth light shielding cotton 740, and the fourth light shielding cotton 740 is located between the third light shielding cotton 730 and the receiving element 310. The fourth shading cotton 740 is fixed back and forth to prevent the influence of light entering from the side surface, and the accuracy of the detection result is further improved.

Referring to fig. 1 and 8, in an embodiment, the heating module 2 includes a heating member 21, a heat conducting member 22, and a temperature control member 23, the heating member 21 is attached to one side of the heat conducting member 22, the other side of the heat conducting member 22 is provided with a recess for accommodating a reaction chamber 41 of the microfluidic chip 4, and the temperature control member 23 is electrically connected to the heating member 21. The temperature control member 23 controls the temperature required by the heating member 21, the heating member 21 is attached to one side of the heat conducting member 22, the heat generated by the heating member 21 is transferred to the heat conducting member 22, and the heat conducting member 22 transfers the heat to the reaction cavity 41 of the microfluidic chip 4 in the abdicating groove to heat the sample to be measured. The heat-conducting member 22 may be made of aluminum block or other material with good heat transfer performance.

Further, the heating module 2 further comprises a temperature protector 24, and the temperature protector 24 is electrically connected with the temperature control member 23. The temperature protector 24 will overheat in abnormal heating to protect the instrument from damage, so that the surface of the heat conducting member 22 can generate heat uniformly.

In one embodiment, the heating module 2 further includes a mounting seat 25, and the heating member 21 is fixed to the heat conducting member 22; further, a temperature-controlling member 23 is fixed to the heat-conducting member 22; the temperature protector 24 is fixed to the heat-conducting member 22; the heat-conducting member 22 is fixed to the mount 25. The mounting seat 25 is connected with a fixing seat 40 of the multi-channel fluorescence detection structure; one side of the heat conducting member 22 having the receding groove faces the fixing seat 40, and a mounting groove for mounting the microfluidic chip 4 is formed between the fixing seat 40 and the mounting seat 25 and between the fixing seat and the heat conducting member 22.

Further, the lamp panel 50 is fixed to the fixing base 40 through supporting plates 55 at two sides; the controller 30 is connected with the fixed seat 40 through the supporting plate 55; the fixing seat 40 plays a role of fixing and supporting. After all elements of the constant-temperature fluorescence detector are assembled in place, the position is kept stable, calibration is not needed before use, and the constant-temperature fluorescence detector is simple to maintain, while a motion module of the traditional equipment needs calibration and reset after being used for one period, and has certain influence on the service life of the equipment; and the constant-temperature fluorescence detector is used for static detection, so that the movement time is reduced, the result is directly read, and the detection efficiency is improved.

Referring to fig. 4 to 7, in an embodiment, the constant temperature fluorescence detector further includes a cover plate 3, an insertion hole 13 for inserting the microfluidic chip 4 is formed in the housing 1, and the cover plate 3 is rotatably connected to the housing 1 and is used for opening or closing the insertion hole 13. The cover plate 3 is rotatably connected with the shell 1 through a rotating shaft. Further, a control switch 11 and an indicator light 12 are disposed on the housing 1, and the control switch 11 and the indicator light 12 are electrically connected to the controller 30 respectively. Three indicator lights 12 are shown in fig. 4-6 for indicating power, positive and negative, respectively.

Referring to fig. 7 and 9, in one embodiment, a power supply interface 14 is disposed on the housing 1, and the power supply interface 14 is electrically connected to the power module 16 to provide power for the operation of the constant temperature fluorescence detector. The input module controls the instrument to start as the control switch 11, the controller 30 controls the heating module 2 to heat the sample to be detected and controls the detection module as the lamp panel 50 to enable the optical module 10 to emit exciting light, and the fluorescence generated by the reaction of the sample to be detected is transmitted to the receiving part 310 to be judged. The controller 30 performs data transmission through a bluetooth module or a line. The result of the detection is shown by a display module 17, such as an indicator light 12. The casing 1 is further provided with a USB interface 15 for data interaction between the controller 30 and an external device. The controller 30 may implement data processing using an MCU.

Referring to fig. 1-9, the working principle and process of the above embodiment are as follows:

inserting the micro-fluidic chip 4 to be detected into the mounting groove through the insertion hole 13, closing the cover plate 3, starting detection according to the control switch 11, enabling the indicator lamp 12 to jump negatively and positively, and lighting the corresponding indicator lamp 12 after the detection is finished; the heating module 2 is started, the temperature of the reaction cavity 41 of the heating module 2 is controlled, the optical module 10 inside the housing 1 is polished until the reaction cavity 41 excites the reagent to react, and a fluorescence value is generated, and the light path passes through the light outlet 420, the second through hole 722, the second optical filter 610, the first through hole 712, the reflection of the first reflecting surface 212, the reflection of the second reflecting surface 222, the third through hole 732 and the fourth through hole 742 in sequence, is finally focused to the receiving part 310 of the controller 30, and is used for judging the fluorescence intensity.

For small POCT (instant on-site detection, even household) and the like, the instrument is small in size and low in cost, the cost of the traditional POCT porous detector is in the thousand-yuan or even ten-thousand-yuan level, the cost is high, the special requirements on the field are generally met, the detection is required in a special laboratory, and the carrying is inconvenient. The constant-temperature fluorescence detector in the embodiment is light, small and convenient to carry; a low-voltage DC power supply can be adopted for supplying power; detecting without special place requirements by a one-step method; the detection can be carried out at home or outdoors, the integrated mode is realized, and the operation is simple; timely detection is carried out, and the process time is reduced; the risk of cross infection caused by the detection process is avoided by in-situ detection; the static detection can be realized, the interpretation is accurate, and no displacement is caused by internal motion; no movement inside, small structure, low cost and stable instrument; the application space is compressed, the size is small, the transportation is convenient, and the carrying is convenient; the volume is not occupied by an optical fiber equal splitting scheme, the light path arrangement and convergence is realized through the reflecting cover 20 with the plurality of first reflecting surfaces 212 and the second reflecting surfaces 222, a plurality of optical detection elements are not required to be configured, the cost is low, the volume is reduced, and the accuracy of a detection result is ensured.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A multi-channel fluorescence detection structure is characterized by comprising

The optical modules are circumferentially arranged around the first axis and are used for respectively emitting exciting light to corresponding samples to be detected;

2. The multi-channel fluorescence detection structure of claim 1, wherein the reflector has a plurality of entrance holes spaced around the first axis, one of the hole walls of the entrance holes is the first reflection surface, the other hole wall is the second reflection surface, the second reflection surface is located in a space surrounded by the first reflection surface, and the plurality of second reflection surfaces are connected to form a multi-pyramid surface with the first axis as a center line.

3. The multi-channel fluorescence detection structure of claim 2, further comprising a fixing base, wherein the fixing base is provided with a plurality of mounting holes and light emitting holes circumferentially arranged around the first axis, the mounting holes and the light emitting holes are arranged in a one-to-one correspondence, the optical modules are arranged in the mounting holes in a one-to-one correspondence, and the light emitting holes are arranged in a correspondence with the first reflecting surfaces;

4. The multi-channel fluorescence detection structure of claim 3, further comprising a lamp panel, wherein the optical module comprises a laser light, and the laser light is mounted or assembled to the lamp panel;

5. The multi-channel fluorescence detection structure of claim 4, further comprising a light shield, wherein the light shield is provided with a plurality of via holes corresponding to the light-emitting holes one by one, the second optical filters are correspondingly installed in the via holes, and the light shield is arranged in the light shield;

and/or the multi-channel fluorescence detection structure further comprises second shading cotton, second through holes corresponding to the light emitting holes one by one are formed in the second shading cotton, through holes are formed in the lamp panel, the second shading cotton is located between the lamp panel and the light shield, and the second shading cotton is covered at the through holes;

6. A constant-temperature fluorescence detector is characterized by comprising a shell, a heating module and the multi-channel fluorescence detection structure as claimed in any one of claims 1 to 5, wherein the heating module and the multi-channel fluorescence detection structure are arranged in the shell, and a mounting groove for mounting a micro-fluidic chip is formed between the heating module and the multi-channel fluorescence detection structure.

7. The constant-temperature fluorescence detector according to claim 6, wherein the heating module comprises a heating element, a heat conducting element and a temperature controlling element, the heating element is attached to one side of the heat conducting element, the other side of the heat conducting element is provided with a yielding groove for accommodating the reaction chamber of the microfluidic chip, and the temperature controlling element is electrically connected with the heating element;

8. The constant-temperature fluorescence detector according to claim 7, wherein the heating module further comprises a mounting seat, the heating element is fixed to the heat conducting element, the heat conducting element is fixed to the mounting seat, the mounting seat is connected with a fixing seat of the multi-channel fluorescence detection structure, and a lamp panel of the multi-channel fluorescence detection structure is fixed to the fixing seat through a supporting plate.

9. The constant-temperature fluorescence detector according to claim 8, further comprising a cover plate, wherein the housing is provided with an insertion hole for inserting the microfluidic chip, and the cover plate is rotatably connected with the housing and is used for opening or closing the insertion hole.

10. The constant-temperature fluorescence detector according to claim 8, wherein the housing is provided with a control switch and an indicator light, and the control switch and the indicator light are respectively electrically connected to the controller.