CN110373312A - A kind of constant-temperature amplification nucleic acid detection apparatus and detection method - Google Patents
A kind of constant-temperature amplification nucleic acid detection apparatus and detection method Download PDFInfo
- Publication number
- CN110373312A CN110373312A CN201910779673.4A CN201910779673A CN110373312A CN 110373312 A CN110373312 A CN 110373312A CN 201910779673 A CN201910779673 A CN 201910779673A CN 110373312 A CN110373312 A CN 110373312A
- Authority
- CN
- China
- Prior art keywords
- chip
- control module
- module
- temperature
- temperature control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 73
- 230000003321 amplification Effects 0.000 title claims abstract description 51
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 51
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 31
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 31
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 238000012360 testing method Methods 0.000 claims abstract description 30
- 238000010790 dilution Methods 0.000 claims abstract description 21
- 239000012895 dilution Substances 0.000 claims abstract description 21
- 238000004458 analytical method Methods 0.000 claims abstract description 11
- 238000012545 processing Methods 0.000 claims abstract description 9
- 238000012546 transfer Methods 0.000 claims abstract description 8
- 239000012530 fluid Substances 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 12
- 238000009396 hybridization Methods 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 7
- 238000007865 diluting Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 230000001960 triggered effect Effects 0.000 claims description 3
- 238000013021 overheating Methods 0.000 claims description 2
- 230000001681 protective effect Effects 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 abstract description 3
- 239000010408 film Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 238000010009 beating Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000012409 standard PCR amplification Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of constant-temperature amplification nucleic acid detection apparatus and detection method, nucleic acid detection apparatus includes: chip, temperature control module, motion module, detection module and control module, and control module is connect with motion module, temperature control module and detection module.Chip is set on temperature control module and is able to carry out sample amplification, dilution and colour developing；Temperature control module is set in motion module and carries out heating and keeping constant temperature to chip；Motion module drives the chip motion to different functional locations, and can in driving chip liquid transfer；Detection module can take pictures to chip；Control module can receive the picture of detection module, carry out processing analysis and output test result to picture.Detection device of the present invention integrates sample constant-temperature amplification, dilution, colour developing, image recognition and analytic function, greatlies simplify apparatus structure, reduces costs, small in size, improves the portability and operability of instrument.
Description
Technical field
The present invention relates to nucleic acid detection technique field more particularly to a kind of constant-temperature amplification nucleic acid detection apparatus and detection sides
Method.
Background technique
Molecular diagnosis market is based primarily upon the tissue sample pretreatment hand of various large-scale instrument and equipments and tediously long complexity at present
Section, it is most of to complete detection 3 kinds of equipment of needs, instrument for extracting nucleic acid, amplification instrument and scanner, with costly, time-consuming, target
Inaccuracy, the disadvantages of popularization is not high.
Wherein, real-time fluorescence quantitative PCR (polymerase chain reaction) uses very universal, traditional inspection in detection of nucleic acids
Survey mode is that eight townhouse PCR pipes or 96 orifice plates are carried out with heating and cooling and real-time fluorescence detection, and not only power consumption is high for entire instrument, and
And equipment instrument is big, structure is complicated, detection time is long.
Therefore, prior art Shortcomings need to improve.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of constant-temperature amplification nucleic acid detection apparatus and detection side
Method.
Technical scheme is as follows: providing a kind of constant-temperature amplification nucleic acid detection apparatus, comprising: chip, temperature control module,
Motion module, detection module and control module, the control module and the motion module, temperature control module and detection module connect
It connects.
The chip is set on the temperature control module, and the chip is able to carry out the amplified reaction of sample, expands sample
Dilution and chromogenic reaction.
The temperature control module is set in the motion module, can be heated to the chip and chip is made to keep permanent
Temperature.
The motion module is able to drive the temperature control module and then drives the chip motion to different functional locations,
And the transfer of liquid in the chip can be driven.
The detection module can take pictures to the chip.
The control module can receive the picture of the detection module, carry out processing analysis to picture and export detection knot
Fruit.
Further, the chip includes: serum cap, upper casing, main component, gasket, test strips and bottom case, the upper casing
Internal after closing with the bottom case lid to form a cavity, the main component, gasket and the test strips are respectively positioned in the cavity,
The gasket corresponds to the main component setting, and the main component is equipped with amplified reaction area and liquid storage area, the liquid storage area
Bottom is equipped with a sealing film, and the gasket corresponds to the liquid storage area equipped with a groove, and the groove is interior to be equipped with a squeezing passage,
The squeezing passage and amplified reaction area connection, the bottom of the groove can deform under external force, described
Sealing film is located in the groove, and the bottom case corresponds to the groove equipped with a first through hole, and the upper casing corresponds to the amplification
Reaction zone is equipped with one second through-hole, and the serum cap is located above the upper casing and covers second through-hole, the test strips
One end be fixed on the gasket far from this one end of the groove, the main component is equipped with one close to this one end of the test strips
Fluid channel, the fluid channel and amplified reaction area connection, the other end of the test strips are equipped with chromogenic reaction area, it is described on
Shell corresponds to the chromogenic reaction area equipped with a windowing, and the tail end of the upper casing is equipped with cog region.
The temperature control module includes: heater, temperature sensor, chip pocket, temperature overheating protective switch, chip cover board
And elastic component, the chip are inserted in the chip pocket, the elastic component can block the chip.
The motion module includes: driving motor, guiding mechanism and link mechanism, and the temperature control module is mounted on Guiding machine
On structure, the driving motor can drive the guiding mechanism to move, and then drive the temperature control module movement, the connecting rod machine
Structure includes: connecting rod, boss and reset spring, and the middle part of the connecting rod is fixed on the temperature control module by shaft, the company
Bar includes contact jaw and free end, and the contact jaw passes through the bottom plate of the chip pocket and protrudes into the first through hole and institute
The bottom surface contact of groove is stated, the connecting rod can rotate under the drive of the guiding mechanism around the shaft, the freedom
End can contact during rotation with the boss, and the boss resists the free end of the connecting rod, and the contact jaw turns upwards
Bottom surface that is dynamic and then oppressing the groove, the bottom surface of the groove concave to destroy the sealing film after being oppressed,
Described reset spring one end is fixed on the temperature control module, and the other end is fixed on the connecting rod, and the connecting rod passes through described
Reset spring resets.
The detection module is set to the surface of the temperature control module, and the detection module includes: light source and photomoduel.
The control module includes: control circuit board and micro computer, the control circuit board and the driving motor, temperature control
Module, light source and micro computer connection, the micro computer are connect with the camera.
Further, the photomoduel includes camera and camera lens, and the camera is CCD camera or COMS camera；It is described
Boss is the inclined-plane or curved surface tilted down close to the end face of this side of connecting rod；The chip is micro-fluidic chip, described
Cog region is two dimensional code.
It further, further include shell, the touching display screen set on the cover top portion, beating set on the outer casing back
Impression block and movable door set on the shell front side, the touching display screen and the print module with the control mould
Block connection, the activity door are installed on the housing by rotary components, and the motion module is able to drive the chip
The door is opened, the chip is stretched out.
Further, the rotary components include torsional spring and pin shaft, can oneself by the drive of the torsional spring door
Dynamic closure.
The present invention also provides a kind of nucleic acid detection methods, comprising the following specific steps
Chip equipped with sample is put into chip pocket by step S1；
Step S2 scans the two dimensional code of chip；
Step S3, carries out amplification reaction sample；
Step S4, dilution amplification sample；
Step S5 carries out hybridization and chromogenic reaction to amplification sample；
Step S6 carries out photo acquisition to colour developing area；
Step S7, picture processing analysis and result output.
Further, drive temperature control module by chip motion to bar code scan by motion module in the step S2
Area, detection module scan the two dimensional code of chip, and image in 2 D code is sent to control module；Pass through control in the step S3
Module control temperature control module heats chip with stationary temperature, and keeps the temperature in the case where being heated to certain temperature to chip, makes
Sample carries out amplification reaction.
Further, in the step S4 after the completion of amplification, motion module drives temperature control module and then drives chip fortune
Dynamic, the free end of connecting rod resists the front end of boss, and connecting rod is triggered and rotates around the axis, and is rotated up the contact jaw of connecting rod crowded
The bottom portion of groove for pressing gasket in chip destroys the sealing film of liquid storage trench bottom, then the dilution outflow in reservoir, and leads to
It crosses squeezing passage and flows into amplified reaction area, dilution amplification sample.
Further, in the step S5 after having diluted amplification sample, motion module continues to move, and drives temperature control module
And then chip is driven to continue to move, the free end of connecting rod is moved down along the inclined-plane of boss, and the contact jaw of connecting rod, which continues up, to be turned
It is dynamic, continue to squeeze bottom portion of groove, and then squeeze the squeezing passage of inside grooves, the amplification sample after diluting amplified reaction area is logical
The chromogenic reaction area that fluid channel flows into test strips is crossed, after reacting a period of time, completes hybridization and chromogenic reaction.
Further, light source is opened by micro computer control, together when hybridizing with after the completion of chromogenic reaction in the step S6
When control camera take pictures to the chromogenic reaction area of chip, obtain chip chromogenic reaction area picture.
In the step S7 after camera photographed the picture in chromogenic reaction area, micro computer obtains picture from camera, carries out
Analysis processing finally will test result and be carried out by touch display screen the results show that being exported simultaneously by print module.
Using the above scheme, detection device of the present invention integrates sample constant-temperature amplification, dilution, colour developing, image recognition and analysis
Function, compact overall structure is small in size, convenient for detection operation；The integrated chip total overall reaction system of reagent, by liquid reagent
It is encapsulated in shell with test strips, and the active transfer of liquid may be implemented, chip structure is compact, and small in size, entire reagent is anti-
Answering test process all is to carry out in the chip, and chip is disposable feed consumption, can be to avoid internal liquid overflow checking device
Biological risk；Temperature control module reduces instrument power consumption, energy saving；Motion module can be achieved at the same time in the movement and chip of chip
The active of liquid is shifted, do not need additional sample charging mechanism, and wiper mechanism realizes that liquid shifts, and greatlies simplify instrument knot
Structure reduces instrument cost, small in size, improves the portability and operability of instrument.
Detailed description of the invention
Fig. 1 is the schematic diagram of constant-temperature amplification nucleic acid detection apparatus of the present invention；
Fig. 2 is the structural schematic diagram of constant-temperature amplification nucleic acid detection apparatus of the present invention；
Fig. 3 is the schematic diagram of internal structure of constant-temperature amplification nucleic acid detection apparatus of the present invention；
Fig. 4 is the structural schematic diagram of chip in constant-temperature amplification nucleic acid detection apparatus of the present invention；
Fig. 5 is the sectional view of chip in constant-temperature amplification nucleic acid detection apparatus of the present invention；
Fig. 6 is the flow chart of present invention detection nucleic acid.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
It please refers to Fig.1 to Fig.3, the present invention provides a kind of constant-temperature amplification nucleic acid detection apparatus, comprising: chip 1, temperature control module
2, motion module 3, detection module 4 and control module 5, the control module 5 and the motion module 3, temperature control module 2 and detection
Module 4 connects.The chip 1 is set on the temperature control module 2, and the chip 1 is able to carry out the amplified reaction of sample, amplification sample
The dilution and chromogenic reaction of product.The temperature control module 2 is set in the motion module 3, can be heated simultaneously to the chip 1
Chip 1 is set to keep constant temperature.The motion module 3 is able to drive the temperature control module 2 and then the chip 1 is driven to move to difference
Functional location, and the transfer of liquid in the chip 1 can be driven.The detection module 4 can clap the chip 1
According to.The control module 5 can receive the picture of the detection module 4, carry out processing analysis and output test result to picture.
The nucleic acid-extracting apparatus further includes shell 6, the touching display screen 7 at the top of the shell 6, is set to 6 rear end of shell
Print module 8 and movable door 9 set on 6 front side of the shell, the touching display screen 7 and the print module 8 with
The control module 5 connects, and the activity door 9 is mounted on the shell 6 by rotary components 10, the motion module 3
It is able to drive the chip 1 and opens the door 9, the chip 1 is stretched out.The rotary components 10 include torsional spring and pin shaft,
It can be closed automatically by the drive of the torsional spring door 9.It is permanent that constant-temperature amplification nucleic acid detection apparatus of the present invention integrates sample
Temperature amplification, dilution, colour developing, image recognition and analytic function, compact overall structure is small in size, convenient for detection operation.
Fig. 4 and Fig. 5 are please referred to, the chip 1 includes: serum cap 11, upper casing 12, main component 13, gasket 14, test strips
15 and bottom case 16.The upper casing 12 is internal after closing with the bottom case 16 lid to form a cavity, the main component 13,14 and of gasket
Test strips 15 are respectively positioned in the cavity.The corresponding main component 13 of the gasket 14 is arranged, and the main component 13 is equipped with
Amplified reaction area 17 and liquid storage area 18, the bottom of the corresponding main component 13 of the liquid storage area 18 is equipped with a sealing film 131, described
Sealing film 131 is thin film, this film can be destroyed under the extruding of external force, to make dilute in the liquid storage area 18
Liquid stream is released to go out.The corresponding liquid storage area 18 of the gasket 14 is equipped with a groove 141, and it is logical that an extruding is equipped in the groove 141
Road 142, the squeezing passage 142 and 17 connection of amplified reaction area.Specifically, in the present embodiment, the gasket 14 is
The bottom of rubber mat, the groove 141 can deform under external force, convenient for squeezing the squeezing passage 142.It is described close
Sealer 131 is located in the groove 141, and the corresponding groove 141 of the bottom case 16 is equipped with a first through hole 161, external structure
The bottom for arriving the groove 141 can be contradicted by the first through hole 161, and the groove 141 is squeezed.On described
The corresponding amplified reaction area 17 of shell 12 is equipped with one second through-hole 121, and the serum cap 11 is located at 12 top of upper casing and covers
The second through-hole 121 is stated in residence, by opening and sample can be added to institute by second through-hole 121 serum cap 11
State amplified reaction area 17.One end of the test strips 15 is fixed on the gasket 14 far from described this one end of groove 141, described
Main component 13 is equipped with a fluid channel 132, the fluid channel 132 and the amplified reaction area 17 close to described this one end of test strips 15
The entrance of connection, the fluid channel 132 is set on the side wall in the amplified reaction area 17, and the outlet of the fluid channel 132 is close
The reagent strip 15 is arranged.The other end of the test strips 15 is equipped with chromogenic reaction area 19, the corresponding colour developing of the upper casing 12
Reaction zone 19 is equipped with a windowing 122, after the completion of amplified reaction, will be diluted to sample, described recessed by squeezing after dilution
The bottom of slot 141 squeezes the squeezing passage 142 in turn, keeps the amplification sample after the dilution in the amplified reaction area 17 logical
It crosses the fluid channel 132 to flow in the test strips 15, and then carries out chromogenic reaction at the chromogenic reaction area 19, finally may be used
To pass through the final colour developing result of 122 observation of windowing.The tail end of the upper casing 12 is equipped with cog region 123, specifically, this implementation
Cog region described in example is two dimensional code, is taken pictures by being in advance scanned the corresponding two dimensional code of the chip 1, is uploaded to described
Control module is convenient for subsequent identification chip.The chip 1 is integrated with the total overall reaction system of reagent, including sample amplification, dilution
It develops the color with hybridization, liquid reagent and test strips is encapsulated in shell, and by squeezing the squeezing passage 142 and the miniflow
Road 132 may be implemented the transfer of liquid, the chip 1 it is compact-sized, small in size, entire test process is all in the chip
It is carried out in 1, and the chip 1 is disposable feed consumption, it can be to avoid the biological risk of internal liquid overflow checking device.
It please refers to Fig.1 to Fig.3, the temperature control module 2 includes: heater 21, temperature sensor 22, chip pocket 23, temperature
Heat portective switch, chip cover board 24 and elastic component are spent, the chip 1 is inserted in the chip pocket 23, the elastic component energy
Chip 1 described in enough clampings, prevents the chip 1 from loosening.The temperature control module 2 uses silica gel heating film and PID temperature control system phase
Cooperation is more uniform temperature, temperature control is more accurate, compared with standard PCR amplification, reduces to realize the constant temperature to the chip 1
The power consumption of detection device, has saved the energy.
Referring to Fig. 1 to Fig. 3, the motion module 3 includes: driving motor 31, guiding mechanism 32 and link mechanism
33, the temperature control module 2 is mounted on guiding mechanism 32, and the driving motor 31 can drive the guiding mechanism 32 to move,
And then the temperature control module 2 is driven to move, the link mechanism includes: connecting rod 33, boss 34 and reset spring 35, the connecting rod
33 middle part is fixed on the temperature control module 2 by shaft 333, and the connecting rod 33 includes contact jaw 331 and free end 332,
The bottom plate that the contact jaw 331 passes through the chip pocket 23 is contacted with the bottom surface of the groove 141, and the connecting rod 33 can
Under the drive of the guiding mechanism 32 around the shaft 333 rotate, the free end 332 during rotation can with it is described convex
Platform 34 contacts, and specifically, the boss 34 is the inclined-plane or curved surface tilted down close to the end face of 33 this side of connecting rod, tiltedly
Bottom end b high of the top a of face or curved surface than inclined-plane or curved surface.The top a of the boss 34 resists the free end of the connecting rod 33
332, the contact jaw 331 is rotated up the bottom surface that can be passed through the first through hole 161 and then oppress the groove 141,
The bottom surface of the groove 141 concaves to destroy the sealing film 131 after being oppressed, and makes the dilution in the liquid storage area 18
Liquid stream goes out；The further rotation of connecting rod 33, the free end 332 are continued along inclined-plane slide downward, and the contact jaw 331 continues
It squeezes the groove 141 and then squeezes the squeezing passage 142, dilution is made to flow into the expansion by the squeezing passage 142
Increase 17 diluting reaction sample of reaction zone；The further rotation of connecting rod 33, when the free end reaches the bottom end b of the boss 34
Behind place, the contact jaw 331 squeezes the squeezing passage 142, makes to be located at the reaction after being diluted in the amplified reaction area 17
Sample is flowed in the test strips 15 by the fluid channel 132, and then is flow to the chromogenic reaction area 19 and carried out chromogenic reaction.
Described 35 one end of reset spring is fixed on the temperature control module 2, and the other end is fixed on the connecting rod 33, anti-when completing colour developing
Ying Hou, the driving motor 31 drive the guiding mechanism 32 to reset to drive the temperature control module 2 to retreat, at this time the company
Bar 33 is realized by the direction elastic force of the reset spring 35 to be resetted.The motion module 3 is by a driving motor
31 realize movement of the chip 1 in different location, and the link mechanism 33 may be implemented liquid in the chip 1 and actively turn
The function of shifting, does not need additional sample charging mechanism, and wiper mechanism realizes that liquid shifts, and greatlies simplify the knot of detection device
Structure reduces costs, small in size, improves the portability and operability of detection device.
Referring to Fig. 1 to Fig. 3, the detection module 4 is set to the surface of the temperature control module 2, the detection mould
Block 4 includes: light source 41 and photomoduel 42, and the photomoduel 42 includes camera and camera lens, the camera be CCD camera or
COMS camera.After the completion of chromogenic reaction, the control module 5 control the detection module 4 to the chromogenic reaction area 19 into
Row take pictures, and by image be uploaded to the control module 5 carry out analysis and as the result is shown.What the use of detection module 4 was taken pictures
Mode is detected, simple and convenient.The control module 5 includes: control circuit board and micro computer 51, the control circuit board with
The driving motor 31, temperature control module 2, light source and micro computer 51 connect, and the micro computer 51 is connect with the camera, the phase
After machine takes the image in the chromogenic reaction area 19, image transmitting is given to the micro computer 51, the micro computer 51 carries out figure
As analyzing, and it is corresponding with chip two dimensional code gathered in advance, analysis result is then sent to the touching display screen 7 and is tied
Fruit shows, while can be printed result by the print module 8.
Fig. 1 to Fig. 6 is please referred to, the present invention also provides a kind of nucleic acid detection methods, specifically, using above-mentioned in the present embodiment
Nucleic acid-extracting apparatus carries out detection of nucleic acids, comprising the following specific steps
Chip equipped with sample is put into chip pocket by step S1.
Step S2, scans the two dimensional code of chip, and through-hole control module controls motion module for chip motion to bar code scan
The two dimensional code of chip is scanned in area.
Step S3, carries out amplification reaction sample.Temperature control module is controlled with stationary temperature to chip by control module
It is heated, and chip is kept the temperature in the case where being heated to certain temperature, carry out amplification reaction sample.
Step S4, dilution amplification sample.After the completion of amplified reaction, motion module drives temperature control module and then drives chip
Movement, the free end of connecting rod resist the front end of boss, and connecting rod, which is triggered, to be rotated around shaft, turn the contact jaw of connecting rod upwards
Bottom portion of groove that is dynamic and then squeezing gasket in chip, the sealing film of liquid storage trench bottom is destroyed, then the dilution in reservoir
Outflow, and amplified reaction area, dilution amplification sample are flowed by squeezing passage.
Step S5 carries out hybridization and chromogenic reaction to amplification sample.After having diluted amplification sample, motion module is after reforwarding
It is dynamic, it drives temperature control module and then chip is driven to continue to move, the free end of connecting rod is moved down along the inclined-plane of boss, and connecting rod connects
Contravention continues up rotation, continues to squeeze bottom portion of groove, and then squeeze the squeezing passage of inside grooves, dilutes amplified reaction area
Amplification sample afterwards flows into the chromogenic reaction area in test strips by fluid channel, after reacting a period of time, completes hybridization and colour developing
Reaction.
Step S6 carries out photo acquisition to colour developing area.When hybridizing with after the completion of chromogenic reaction, opened by micro computer control
Light source, while controlling camera and taking pictures to the chromogenic reaction area of chip, obtain the picture in chip chromogenic reaction area.
Step S7, picture processing analysis and result output.After camera photographed the picture in chromogenic reaction area, micro computer is from phase
Picture is obtained in machine, is analyzed and processed, and finally be will test result and is carried out by touch display screen the results show that simultaneously by beating
Impression block output.
In conclusion detection device of the present invention integrates sample constant-temperature amplification, dilution, colour developing, image recognition and analytic function,
Compact overall structure, it is small in size, convenient for detection operation；The integrated chip total overall reaction system of reagent, by liquid reagent and examination
Paper slip is encapsulated in shell, and the active transfer of liquid may be implemented, and chip structure is compact, small in size, and entire reagent reaction is surveyed
Examination process is all to carry out in the chip, and chip is disposable feed consumption, can be to avoid the biology of internal liquid overflow checking device
Risk；Temperature control module reduces instrument power consumption, energy saving；Liquid in the movement and chip of chip can be achieved at the same time in motion module
Active transfer, do not need additional sample charging mechanism, wiper mechanism realizes that liquid shifts, and greatlies simplify apparatus structure, drops
Low instrument cost, it is small in size, improve the portability and operability of instrument.
The above is merely preferred embodiments of the present invention, be not intended to restrict the invention, it is all in spirit of the invention and
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within principle.
Claims (10)
1. a kind of constant-temperature amplification nucleic acid detection apparatus characterized by comprising chip, temperature control module, motion module, detection mould
Block and control module, the control module are connect with the motion module, temperature control module and detection module,
The chip is set on the temperature control module, and the chip is able to carry out the amplified reaction of sample, expands the dilution of sample
And chromogenic reaction；
The temperature control module is set in the motion module, can be heated to the chip and chip is made to keep constant temperature；
The motion module is able to drive the temperature control module and then drives the chip motion to different functional locations, and energy
Enough drive the transfer of liquid in the chip；
The detection module can take pictures to the chip；
The control module can receive the picture of the detection module, carry out processing analysis and output test result to picture.
2. constant-temperature amplification nucleic acid detection apparatus according to claim 1, which is characterized in that the chip include: serum cap,
Upper casing, main component, gasket, test strips and bottom case, the upper casing and the bottom case lid are internal after closing to form a cavity, the master
Body part, gasket and the test strips are respectively positioned in the cavity, and the gasket corresponds to the main component setting, the main body
Part is equipped with amplified reaction area and liquid storage area, and the bottom of the liquid storage area is equipped with a sealing film, and the gasket corresponds to the storage
Liquid zone is equipped with a groove, and a squeezing passage, the squeezing passage and amplified reaction area connection are equipped in the groove, described
The bottom of groove can deform under external force, and the sealing film is located in the groove, described in the bottom case correspondence
Groove is equipped with a first through hole, and the upper casing corresponds to the amplified reaction area equipped with one second through-hole, and the serum cap is located at institute
State above upper casing and cover second through-hole, one end of the test strips be fixed on the gasket far from the groove this
End, the main component are equipped with a fluid channel, the fluid channel and amplified reaction area connection close to this one end of the test strips,
The other end of the test strips be equipped with chromogenic reaction area, the upper casing correspond to the chromogenic reaction area equipped with one open a window, it is described on
The tail end of shell is equipped with cog region；
The temperature control module includes: heater, temperature sensor, chip pocket, temperature overheating protective switch, chip cover board and bullet
Property part, the chip are inserted in the chip pocket, and the elastic component can block the chip；
The motion module includes: driving motor, guiding mechanism and link mechanism, and the temperature control module is mounted on guiding mechanism
On, the driving motor can drive the guiding mechanism to move, and then drive the temperature control module movement, the link mechanism
It include: connecting rod, boss and reset spring, the middle part of the connecting rod is fixed on the temperature control module by shaft, the connecting rod
Including contact jaw and free end, the contact jaw pass through the bottom plate of the chip pocket and protrude into the first through hole with it is described
The bottom surface of groove contacts, and the connecting rod can rotate under the drive of the guiding mechanism around the shaft, the free end
It can be contacted during rotation with the boss, the boss resists the free end of the connecting rod, and the contact jaw is rotated up
And then the bottom surface of the groove is oppressed, the bottom surface of the groove concaves to destroy the sealing film, institute after being oppressed
It states reset spring one end to be fixed on the temperature control module, the other end is fixed on the connecting rod, and the connecting rod passes through described multiple
Position spring reset；
The detection module is set to the surface of the temperature control module, and the detection module includes: light source and photomoduel；
The control module includes: control circuit board and micro computer, the control circuit board and the driving motor, temperature control mould
Block, light source and micro computer connection, the micro computer are connect with the camera.
3. constant-temperature amplification nucleic acid detection apparatus according to claim 2, which is characterized in that the photomoduel includes camera
And camera lens, the camera are CCD camera or COMS camera；The boss is to tilt down close to the end face of this side of connecting rod
Inclined-plane or curved surface；The chip is micro-fluidic chip, and the cog region is two dimensional code.
4. constant-temperature amplification nucleic acid detection apparatus according to claim 1, which is characterized in that further include shell, set on described
The touching display screen of cover top portion, the print module set on the outer casing back and the movable door set on the shell front side,
The touching display screen and the print module are connect with the control module, and the activity door is installed by rotary components
On the housing, the motion module is able to drive the chip and opens the door, and the chip is stretched out.
5. constant-temperature amplification nucleic acid detection apparatus according to claim 4, which is characterized in that the rotary components include torsional spring
And pin shaft, it can be closed automatically by the drive of the torsional spring door.
6. a kind of nucleic acid detection method, which is characterized in that comprising the following specific steps
Chip equipped with sample is put into chip pocket by step S1；
Step S2 scans the two dimensional code of chip；
Step S3, carries out amplification reaction sample；
Step S4, dilution amplification sample；
Step S5 carries out hybridization and chromogenic reaction to amplification sample；
Step S6 carries out photo acquisition to colour developing area；
Step S7, picture processing analysis and result output.
7. nucleic acid detection method according to claim 6, which is characterized in that driven in the step S2 by motion module
For temperature control module in turn by chip motion to bar code scan area, detection module scans the two dimensional code of chip, and image in 2 D code is sent out
It send to control module；Temperature control module is controlled by control module in the step S3 to heat chip with stationary temperature,
And chip is kept the temperature in the case where being heated to certain temperature, carry out amplification reaction sample.
8. nucleic acid detection method according to claim 6, which is characterized in that in the step S4 after the completion of amplification, fortune
Dynamic model block drives temperature control module and then drives chip motion, and the free end of connecting rod resists the front end of boss, connecting rod be triggered and around
Shaft rotation, makes the contact jaw of connecting rod be rotated up the bottom portion of groove for squeezing gasket in chip, by the sealing of liquid storage trench bottom
Film destroys, then the dilution outflow in reservoir, and flows into amplified reaction area, dilution amplification sample by squeezing passage.
9. nucleic acid detection method according to claim 6, which is characterized in that when having diluted amplification sample in the step S5
Afterwards, motion module continues to move, and drives temperature control module and then chip is driven to continue to move, the free end of connecting rod is along the inclined-plane of boss
It moves down, the contact jaw of connecting rod continues up rotation, continues to squeeze bottom portion of groove, and then the extruding for squeezing inside grooves is logical
Road, the amplification sample after diluting amplified reaction area flows into the chromogenic reaction area in test strips by fluid channel, when reacting one section
Between after, complete hybridization and chromogenic reaction.
10. nucleic acid detection method according to claim 6, which is characterized in that when hybridization and colour developing are anti-in the step S6
After the completion of answering, light source is opened by micro computer control, while controlling camera and taking pictures to the chromogenic reaction area of chip, obtain core
The picture in piece chromogenic reaction area；
In the step S7 after camera photographed the picture in chromogenic reaction area, micro computer obtains picture from camera, is analyzed
Processing finally will test result and be carried out by touch display screen the results show that being exported simultaneously by print module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910779673.4A CN110373312B (en) | 2019-08-22 | 2019-08-22 | Constant-temperature amplification nucleic acid detection device and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910779673.4A CN110373312B (en) | 2019-08-22 | 2019-08-22 | Constant-temperature amplification nucleic acid detection device and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110373312A true CN110373312A (en) | 2019-10-25 |
| CN110373312B CN110373312B (en) | 2022-11-22 |
Family
ID=68260439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910779673.4A Active CN110373312B (en) | 2019-08-22 | 2019-08-22 | Constant-temperature amplification nucleic acid detection device and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110373312B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305773A (en) * | 2019-08-22 | 2019-10-08 | 深圳市芯思微生物科技有限公司 | A kind of nucleic acid-extracting apparatus and extracting method |
| CN111748463A (en) * | 2020-08-07 | 2020-10-09 | 天津智善生物科技有限公司 | Amplification device applied to nucleic acid detection and detection method based on amplification device |
| WO2021134511A1 (en) * | 2019-12-31 | 2021-07-08 | 苏州缔因安生物科技有限公司 | Full-integrated small-scale chip type digital pcr detection system and detection method |
| CN113324985A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Centrifugal micro-fluidic detection device and centrifugal micro-fluidic detection system |
| WO2021254505A1 (en) * | 2020-06-19 | 2021-12-23 | 安徽为臻生物工程技术有限公司 | Anti-contamination testing device and method for rapid testing of nucleic acid amplification product, and use thereof |
| CN113930322A (en) * | 2021-10-12 | 2022-01-14 | 江苏汇先医药技术有限公司 | LAMP detector and control method |
| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114100722A (en) * | 2021-12-27 | 2022-03-01 | 北京梓晶生物科技有限公司 | Portable full-automatic nucleic acid constant temperature amplification device |
| CN114317224A (en) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | Nucleic acid detection host and nucleic acid detection equipment |
| WO2023060850A1 (en) * | 2021-10-12 | 2023-04-20 | 江苏汇先医药技术有限公司 | Handheld lamp detector and system |
| CN116574603A (en) * | 2023-07-14 | 2023-08-11 | 潍坊安普未来生物科技有限公司 | Isothermal amplification detection device and detection method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630373A (en) * | 2015-02-13 | 2015-05-20 | 博奥生物集团有限公司 | Rapid parallel nucleic acid detection method and system based on micro-fluidic chip |
| CN107043697A (en) * | 2017-06-14 | 2017-08-15 | 北京百康芯生物科技有限公司 | A kind of multi-channel chip constant-temperature amplification detection of nucleic acids instrument |
| WO2019006978A1 (en) * | 2017-07-03 | 2019-01-10 | 南京岚煜生物科技有限公司 | Single-channel chemiluminescence microfluidic chip and detection method thereof |
| CN109943483A (en) * | 2018-11-03 | 2019-06-28 | 宁波大学 | Cell culture apparatus and method based on real-time complementary technology |
| CN209243065U (en) * | 2018-11-22 | 2019-08-13 | 深圳市芯思微生物科技有限公司 | A kind of nucleic acid test card |
-
2019
- 2019-08-22 CN CN201910779673.4A patent/CN110373312B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630373A (en) * | 2015-02-13 | 2015-05-20 | 博奥生物集团有限公司 | Rapid parallel nucleic acid detection method and system based on micro-fluidic chip |
| CN107043697A (en) * | 2017-06-14 | 2017-08-15 | 北京百康芯生物科技有限公司 | A kind of multi-channel chip constant-temperature amplification detection of nucleic acids instrument |
| WO2019006978A1 (en) * | 2017-07-03 | 2019-01-10 | 南京岚煜生物科技有限公司 | Single-channel chemiluminescence microfluidic chip and detection method thereof |
| CN109943483A (en) * | 2018-11-03 | 2019-06-28 | 宁波大学 | Cell culture apparatus and method based on real-time complementary technology |
| CN209243065U (en) * | 2018-11-22 | 2019-08-13 | 深圳市芯思微生物科技有限公司 | A kind of nucleic acid test card |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110305773A (en) * | 2019-08-22 | 2019-10-08 | 深圳市芯思微生物科技有限公司 | A kind of nucleic acid-extracting apparatus and extracting method |
| WO2021134511A1 (en) * | 2019-12-31 | 2021-07-08 | 苏州缔因安生物科技有限公司 | Full-integrated small-scale chip type digital pcr detection system and detection method |
| EP4170010A4 (en) * | 2020-06-19 | 2023-12-27 | Anhui Weizhen Biological Engineering Technology Co. Ltd. | Anti-contamination testing device and method for rapid testing of nucleic acid amplification product, and use thereof |
| WO2021254505A1 (en) * | 2020-06-19 | 2021-12-23 | 安徽为臻生物工程技术有限公司 | Anti-contamination testing device and method for rapid testing of nucleic acid amplification product, and use thereof |
| CN111748463A (en) * | 2020-08-07 | 2020-10-09 | 天津智善生物科技有限公司 | Amplification device applied to nucleic acid detection and detection method based on amplification device |
| CN114317224A (en) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | Nucleic acid detection host and nucleic acid detection equipment |
| CN113324985A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Centrifugal micro-fluidic detection device and centrifugal micro-fluidic detection system |
| CN113930322A (en) * | 2021-10-12 | 2022-01-14 | 江苏汇先医药技术有限公司 | LAMP detector and control method |
| WO2023060851A1 (en) * | 2021-10-12 | 2023-04-20 | 江苏汇先医药技术有限公司 | Lamp tester and control method |
| WO2023060850A1 (en) * | 2021-10-12 | 2023-04-20 | 江苏汇先医药技术有限公司 | Handheld lamp detector and system |
| CN113930322B (en) * | 2021-10-12 | 2024-03-08 | 江苏汇先医药技术有限公司 | LAMP detector and control method |
| CN114047170A (en) * | 2021-11-27 | 2022-02-15 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114047170B (en) * | 2021-11-27 | 2022-08-16 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114100722A (en) * | 2021-12-27 | 2022-03-01 | 北京梓晶生物科技有限公司 | Portable full-automatic nucleic acid constant temperature amplification device |
| CN114100722B (en) * | 2021-12-27 | 2024-06-04 | 北京梓晶生物科技有限公司 | Portable full-automatic nucleic acid isothermal amplification device |
| CN116574603A (en) * | 2023-07-14 | 2023-08-11 | 潍坊安普未来生物科技有限公司 | Isothermal amplification detection device and detection method |
| CN116574603B (en) * | 2023-07-14 | 2023-09-19 | 潍坊安普未来生物科技有限公司 | Isothermal amplification detection device and detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110373312B (en) | 2022-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110373312A (en) | A kind of constant-temperature amplification nucleic acid detection apparatus and detection method | |
| US9061280B2 (en) | Chemical reaction cartridge, its fabrication method, and a chemical reaction cartridge drive system | |
| CN104946510B (en) | Collection nucleic acid amplification and microarray are detected on the micro fluidic device of one | |
| EP3198260B1 (en) | Device for detecting and/or analyzing bio-chip | |
| CN101218032A (en) | System for the integrated and automated analysis of DNA or protein and method for operating said type of system | |
| WO2022134153A1 (en) | Nucleic acid poct testing apparatus and testing method thereof | |
| CN110373309A (en) | A kind of nucleic acid extraction amplification system and molecular detector arrangement | |
| WO2023060851A1 (en) | Lamp tester and control method | |
| CN113652350B (en) | Full-automatic PCR analysis system for molecular diagnosis by using microfluidic chip | |
| CN109055203A (en) | A kind of full-automatic biological chips work station and its detection method | |
| CN103308502B (en) | Handheld general microfluidic chip real-time detection device and application | |
| JP2022058157A (en) | Nucleic acid detection host, and nucleic acid detection device | |
| CN100578224C (en) | Micro fluid control chip for investigating cell surfact marker | |
| CN113598709A (en) | Medicine screening device based on in vivo visible light imaging of living animal | |
| CN210923478U (en) | Real-time fluorescent quantitative PCR instrument for on-site rapid detection | |
| CN112831399A (en) | Automatic detection reagent bottle group, kit, reagent bin and detection method for intelligent hospital | |
| KR20210133713A (en) | Microfludic device and appratus for analyzing sample | |
| CN115747031A (en) | Pathogen nucleic acid analysis apparatus | |
| WO2003092893A1 (en) | Integrated device for carrying out a genetic investigation and relative examining and reading equipment | |
| TW202215032A (en) | Nucleic acid detection disc and nucleic acid detection device | |
| CN112394033A (en) | Novel molecular diagnosis detection equipment | |
| WO2021134511A1 (en) | Full-integrated small-scale chip type digital pcr detection system and detection method | |
| US20220099621A1 (en) | Nucleic acid detection kit and nucleic acid detection device | |
| JP2022058274A (en) | Nucleic acid detection main body and nucleic acid detection device | |
| CN2473212Y (en) | Biological chip analying instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: Building 101, Building C, No. 14 Jinxiu East Road, Xiuxin Community, Kengzi Street, Pingshan District, Shenzhen City, Guangdong Province, 518000 Patentee after: SHENZHEN SINGUWAY BIOTECHNOLOGY Co.,Ltd. Address before: Room B1302, Life Science Park, Shenzhen Chengtou Creative Factory, A Road, Julongshan, Xiuxin Community, Kengzi Street, Pingshan District, Shenzhen, 518000 Patentee before: SHENZHEN SINGUWAY BIOTECHNOLOGY Co.,Ltd. |
|
| CP02 | Change in the address of a patent holder |