CN109943483A - Cell culture apparatus and method based on real-time complementary technology - Google Patents
Cell culture apparatus and method based on real-time complementary technology Download PDFInfo
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- CN109943483A CN109943483A CN201910240633.2A CN201910240633A CN109943483A CN 109943483 A CN109943483 A CN 109943483A CN 201910240633 A CN201910240633 A CN 201910240633A CN 109943483 A CN109943483 A CN 109943483A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 53
- 230000000295 complement effect Effects 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 10
- 230000033001 locomotion Effects 0.000 claims description 22
- 239000012737 fresh medium Substances 0.000 claims description 6
- 238000012544 monitoring process Methods 0.000 claims description 6
- 238000002955 isolation Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000013589 supplement Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000012930 cell culture fluid Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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Abstract
The present invention provides a kind of cell culture apparatus and method based on real-time complementary technology, the cell culture apparatus based on real-time complementary technology include the first container suitable for accommodating cell and its culture solution；Sensor, the sensor are used to detect the parameter of culture solution in the first container；Second container, the second container are connect for accommodating culture solution with the first container；First switch, the first switch for controlled according to the parameter that the sensor exports the first container between second container whether connection；Third container, the third container are connect for accommodating culture solution with the first container；Second switch, the second switch for control the third container between the first container whether connection.The present invention has many advantages, such as that structure is simple and convenient to operate, automates.
Description
Technical field
The present invention relates to cell culture, in particular to cell culture apparatus and method based on real-time complementary technology.
Background technique
Basic technology of the cell culture technology as technical field of bioengineering, to obtain biological product, specific cell
And metabolite provides effective scheme.Cell culture technology, cell culture fluid play the role of it is critical, for providing cell
Nutrient required for cultivating builds the living environment of good a growth and breeding for cell.In incubation, with nutrition
The consumption of ingredient, the nutrient in culture solution will no longer satisfy the needs of cell growth, and as cell physiological constantly becomes
Change, cell metabolism will generate certain toxic effect to cell.Therefore, it is necessary to periodically be that cell replaces culture solution.
Currently, the common cell culture fluid substitute mode in laboratory is realized by cumbersome manual hand manipulation.Cause
This, establishes a set of efficient, and the alternative cell culture apparatus for manually changing liquid operation maintains the relatively steady of cell culture environment
State is a problem in the urgent need to address
Summary of the invention
To solve the deficiency in above-mentioned prior art, the present invention provides a kind of automations, structure simply, operation
Cell culture apparatus easily based on real-time complementary technology.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of cell culture apparatus based on real-time complementary technology, the cell culture apparatus based on real-time complementary technology
The first container including being suitable for accommodating cell and its culture solution；The cell culture apparatus based on real-time complementary technology is further
Include:
Sensor, the sensor are used to detect the parameter of culture solution in the first container；
Second container, the second container are connect for accommodating culture solution with the first container；
First switch, the first switch for controlled according to the parameter that the sensor export the first container with
Whether connection between second container；
Third container, the third container are connect for accommodating culture solution with the first container；
Second switch, the second switch for control the third container between the first container whether connection.
The object of the invention is also to provide the cell culture processes based on above-mentioned cell culture apparatus, the goals of the invention
It has the technical scheme that
According to the cell culture processes of the above-mentioned cell culture apparatus based on real-time complementary technology, the cell culture side
Method the following steps are included:
(A1) in sensor real-time monitoring the first container culture solution parameter, when the parameter reaches threshold value, into step
Suddenly (A2)；
(A2) first switch is opened, and the culture solution in the first container enters in the second container；Second switch is closed, institute
State the first container and second container isolation；
Second switch is opened, and the culture solution in third container is got enter into the first container, and supplement first is held in real time
Culture solution in device.
Compared with prior art, the device have the advantages that are as follows:
1. the automation for realizing culture solution supplements in real time；
Using the parameter of culture solution in sensor real-time monitoring the first container, but when parameter reaches threshold value, need to replace
Culture solution in one container: the culture solution in the first container enters in second container, meanwhile, the fresh medium in third container
Into in the first container, whole-course automation；
2. only needing several containers and switch, structure is simple and convenient to operate.
Detailed description of the invention
Referring to attached drawing, the disclosure of the present invention will become more readily understood.Skilled addressee readily understands that: this
It is only used to illustrate the technical scheme of the present invention for a little attached drawings, and is not intended to limit the scope of protection of the present invention.
In figure:
Fig. 1 is the structure diagram of the cell culture apparatus based on real-time complementary technology of the embodiment of the present invention.
Specific embodiment
Fig. 1 and following description describe optional embodiment of the invention with instruct those skilled in the art how to implement and
Reproduce the present invention.In order to teach the technical scheme of the invention, it has simplified or omitted some conventional aspects.Those skilled in the art answer
The variation or replacement that the understanding is originated from these embodiments will within the scope of the invention.Under those skilled in the art should understand that
Stating feature can combine in various ways to form multiple modifications of the invention.The invention is not limited to following optional as a result,
Embodiment, and be only limited by the claims and their equivalents.
Embodiment 1:
Fig. 1 schematically illustrates the structure of the cell culture apparatus based on real-time complementary technology of the embodiment of the present invention 1
Schematic diagram, as shown in Figure 1, the cell culture apparatus includes:
The first container, the first container are suitable for accommodating cell and its culture solution；
Sensor, such as pressure sensor, the sensor are used to detect the parameter of culture solution in the first container；
Second container, the second container are connect for accommodating culture solution with the first container；
First switch, the first switch for controlled according to the parameter that the sensor export the first container with
Whether connection between second container: if the parameter reaches threshold value, then opening first switch, the culture solution in the first container flows into
In second container, such as parameter is not up to threshold value, then closes the first switch, the first container and second container interval
From；
Third container, the third container are connect for accommodating fresh medium with the first container；
Second switch, the second switch for control the third container between the first container whether connection: such as institute
It states parameter and reaches threshold value, then open second switch, the culture solution in third container flows into the first container, and such as parameter does not reach
To threshold value, then the second switch is closed, the first container and third container keep isolation.
According to the above-mentioned cell culture apparatus based on real-time complementary technology, further, the second switch has more
Kind form, the second switch include:
Movement parts, the movement parts are used to squeeze the first side wall of the third container；The first side wall is using flexible
Material；
The second sidewall of the connection the first container of the third container has intensity threshold: when the third container quilt
When the movement parts squeeze, the pressure value in third container reaches the threshold value, so that described in squeezing when the movement parts
When the first side wall of third container, second sidewall ruptures, and the fresh medium in third container enters in the first container.
It is further, described in order to drive movement parts according to the above-mentioned cell culture apparatus based on real-time complementary technology
Cell culture apparatus further comprises:
Driving unit, the driving unit is for driving the movement parts, as motor drives movement parts such as cam to rotate, or
Movement parts linear motion ground is driven to squeeze the first side wall of third container.
According to the above-mentioned cell culture apparatus based on real-time complementary technology, further, the second switch includes:
Valve, the valve are arranged on the pipeline connected between the first container and third container.
According to the above-mentioned cell culture apparatus based on real-time complementary technology, further, the second switch includes:
Movement parts, the movement parts are used to puncture the side wall of the connection the first container of the third container.
According to the above-mentioned cell culture apparatus based on real-time complementary technology, further, for the ease of in third container
Fresh medium flow into the first container, the side wall of the first container has a through-hole, the side wall of the third container close to
The through-hole.
The cell culture processes of the embodiment of the present invention, namely filled according to the above-mentioned cell culture based on real-time complementary technology
The cell culture processes set, the cell culture processes the following steps are included:
(A1) in sensor real-time monitoring the first container culture solution parameter, when the parameter reaches threshold value, into step
Suddenly (A2)；
(A2) first switch is opened, and the culture solution in the first container enters in the second container；Second switch is closed, institute
State the first container and second container isolation；
Second switch is opened, and the culture solution in third container is got enter into the first container, and supplement first is held in real time
Culture solution in device.
According to above-mentioned cell culture processes, further, the working method of the second switch are as follows:
The side wall of the third container is squeezed, the pressure in the third container increases, so that the company of the third container
The sidewall rupture of the first container is connect, the fresh medium in third container enters in the first container.
Embodiment 2:
According to embodiments of the present invention 1 cell culture apparatus based on real-time complementary technology and the application examples of method.
In the application examples, sensor uses pressure sensor, the pressure of culture solution in real-time monitoring the first container；First
Switch uses valve；The position of second container is lower than the first container, so that the culture solution in the first container only relies on gravity
Ground flows into second container；The first side wall of third container such as bottom wall uses flexible material, and movement parts use cam, the cam
Rotation is driven by motor, so that cam squeezes the bottom wall of the third container in rotation, and then improves in the third container
Pressure, to reach the intensity threshold of second sidewall, to rupture；The second sidewall of third container uses film；Described first holds
The side wall of device has through-hole, and the second sidewall is arranged close to the side of the through-hole.
The cell culture processes of the embodiment of the present invention, namely filled according to the above-mentioned cell culture based on real-time complementary technology
The cell culture processes set, the cell culture processes the following steps are included:
(A1) in pressure sensor real-time monitoring the first container culture solution parameter, when the parameter reaches threshold value, into
Enter step (A2)；
(A2) first switch is opened, i.e., valve is opened, and the culture solution in the first container enters in the second container；Second opens
It closes, i.e., valve is closed, the first container and second container isolation；
Second switch is opened, namely: motor rotation, the bottom wall of the cam extrusion third container of rotation, extruded second
Pressure in container increases to the intensity threshold of second sidewall, is ruptured at this time using the second sidewall of film, the training in third container
The second sidewall that nutrient solution passes through rupture enters in through-hole, finally enters in the first container, supplements in the first container in real time
Culture solution.
Embodiment 3:
The cell culture apparatus and method based on real-time complementary technology of the embodiment of the present invention, as different from Example 2:
1. valve is not only arranged as first switch, on the pipeline on the pipeline of connection the first container and second container
Pump is also set up, to extract the culture solution in the first container out；
2. second switch is tip using the movement parts of linear motion, one end of the movement parts, hold suitable for puncturing third
The side wall of device connecting with the first container, the side wall are attached to the side of the through-hole in the first container；The bottom wall of third container is no longer
Flexible material is used, and uses hard material.
Claims (10)
1. a kind of cell culture apparatus based on real-time complementary technology, the cell culture apparatus packet based on real-time complementary technology
Include the first container for being suitable for accommodating cell and its culture solution；It is characterized by: the cell culture based on real-time complementary technology
Device further comprises:
Sensor, the sensor are used to detect the parameter of culture solution in the first container；
Second container, the second container are connect for accommodating culture solution with the first container；
First switch, the first switch are used to control the first container and second according to the parameter that the sensor exports
Whether connection between container；
Third container, the third container are connect for accommodating culture solution with the first container；
Second switch, the second switch for control the third container between the first container whether connection.
2. the cell culture apparatus according to claim 1 based on real-time complementary technology, it is characterised in that: described second opens
Pass includes:
Movement parts, the movement parts are used to squeeze the first side wall of the third container；The first side wall uses flexible material；
The second sidewall of the connection the first container of the third container has intensity threshold: when the third container is described
When movement parts squeeze, the pressure value in third container reaches the threshold value.
3. the cell culture apparatus according to claim 2 based on real-time complementary technology, it is characterised in that: the cell training
Feeding device further comprises:
Driving unit, the driving unit is for driving the movement parts.
4. the cell culture apparatus according to claim 2 based on real-time complementary technology, it is characterised in that: the movement parts
For cam structure.
5. the cell culture apparatus according to claim 2 based on real-time complementary technology, it is characterised in that: described second side
Wall uses film.
6. the cell culture apparatus according to claim 1 based on real-time complementary technology, it is characterised in that: described second opens
Pass includes:
Valve, the valve are arranged on the pipeline connected between the first container and third container.
7. the cell culture apparatus according to claim 1 based on real-time complementary technology, it is characterised in that: described second opens
Pass includes:
Movement parts, the movement parts are used to puncture the side wall of the connection the first container of the third container.
8. the cell culture apparatus according to claim 1 based on real-time complementary technology, it is characterised in that: described first holds
The side wall of device has through-hole, and the side wall of the third container is close to the through-hole.
9. the cell culture processes of -8 any cell culture apparatus based on real-time complementary technology according to claim 1,
The cell culture processes the following steps are included:
(A1) parameter of culture solution is entered step when the parameter reaches threshold value in sensor real-time monitoring the first container
(A2)；
(A2) first switch is opened, and the culture solution in the first container enters in the second container；Second switch is closed, and described the
One container and second container isolation；
Second switch is opened, and the culture solution in third container is got enter into the first container, is supplemented in the first container in real time
Culture solution.
10. cell culture processes according to claim 9, it is characterised in that: the working method of the second switch are as follows:
The side wall of the third container is squeezed, pressure in the third container increases, so that the connection of the third container the
The sidewall rupture of one container, the fresh medium in third container enter in the first container.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811304040X | 2018-11-03 | ||
| CN201811304040 | 2018-11-03 |
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| Publication Number | Publication Date |
|---|---|
| CN109943483A true CN109943483A (en) | 2019-06-28 |
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ID=67012107
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| CN201910240633.2A Pending CN109943483A (en) | 2018-11-03 | 2019-03-28 | Cell culture apparatus and method based on real-time complementary technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110373312A (en) * | 2019-08-22 | 2019-10-25 | 深圳市芯思微生物科技有限公司 | A kind of constant-temperature amplification nucleic acid detection apparatus and detection method |
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