KR101706750B1 - Multichannel cell culture dish - Google Patents

Multichannel cell culture dish Download PDF

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Publication number
KR101706750B1
KR101706750B1 KR1020150116899A KR20150116899A KR101706750B1 KR 101706750 B1 KR101706750 B1 KR 101706750B1 KR 1020150116899 A KR1020150116899 A KR 1020150116899A KR 20150116899 A KR20150116899 A KR 20150116899A KR 101706750 B1 KR101706750 B1 KR 101706750B1
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South Korea
Prior art keywords
cell culture
connection channel
culture
channel
section
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KR1020150116899A
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Korean (ko)
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이봉기
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전남대학교산학협력단
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

Abstract

The present invention discloses a multi-channel cell culture vessel capable of stably culturing cells by continuously supplying a culture solution, and independently evaluating the characteristics of the cells by independently delivering chemical stimuli such as drugs and the like.
The multi-channel cell culture container of the present invention comprises a culture solution distributing part accommodating a cell culture solution, a first connection channel connected to the culture solution distributing part and connected to the first connection channel, and the cell culture solution and the cells to be cultured are accommodated A second connection channel connected to the cell culture unit and accommodated in the cell culture unit, and a culture solution disposal unit connected to the second connection channel to receive the lung culture solution.

Description

Multichannel cell culture dish [0002]

The present invention relates to a multi-channel cell culture container capable of stably culturing cells by continuously supplying a culture solution and independently evaluating the characteristics of the cells by independently delivering chemical stimuli such as drugs and the like.

Recently, as engineering techniques such as micro and nano manufacturing technology and precision sensor technology have been developed, cell research using this technology is actively being carried out. In other words, it is possible to precisely measure and analyze the composition of the artificial physical environment simulating the micro and nano environment in vivo and the various behaviors of the cells in such environment. This has a great influence on the development of tissue engineering such as regeneration of artificial organs using stem cells as well as simulation of various environments that could not be realized by existing cell and biological research technology.

The ability to more accurately and quickly identify the effects of various biochemical substances on cell behavior is critical in the practical application of new drugs and new materials that are being developed in a variety of ways. In particular, in order to understand the effects of very small amounts of substances on long-term cells, it is essential to measure the change of cells in a certain environment.

In general, in order to maintain various environmental conditions for stable culture of cells, cells and a culture solution are put into a container such as Petri dish or a well plate made of a biocompatible plastic material such as polystyrene, The incubation is continued for a certain period of time in the incubator.

For the long-term cultivation and observation of cells, related experiments are carried out by covering the periphery of the microscope used for observing the cells as a whole and controlling the environment for culturing the cells inside thereof or installing a small incubator on the observation stage of the microscope . However, such conventional techniques use a conventional container as it is, which makes it difficult to exchange the culture medium after a certain period of time, and it is inconvenient to inject various materials.

In addition, it is necessary to obtain a large number of experimental result data under the same condition. However, the existing cell culture container has a limited number of experiments to be performed at one time under the same condition, have.

Korean Patent Laid-Open Publication No. 10-2015-0044409 (disclosed on May 24, 2015)

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a multi-channel cell culture vessel capable of stably performing long-term cell culture and observation of cells by eliminating the inconvenience of exchanging the culture medium. .

It is a further object of the present invention to provide a method for the detection of multiple channels of different types of chemicals, such as independent observations of the effects of different kinds of chemicals on the same cells, And to provide a cell culture container.

It is still another object of the present invention to provide a multi-channel cell culture container capable of obtaining a large amount of experimental data under the same single experimental condition, thereby reducing the time required for experimentation and increasing the reliability of experimental data.

It is still another object of the present invention to provide a multi-channel cell culture container which can be manufactured in a disposable manner through mass production to prevent contamination during cell testing by reuse.

In order to accomplish the object of the present invention as described above, the present invention provides a method for producing a cell culture solution, comprising: a culture solution dispensing part for containing a cell culture solution; a first connection channel connected to the culture solution dispensing part, A cell culture unit in which the cell culture solution and cells to be cultured are accommodated, a second connection channel connected to the cell culture unit and to which the pulmonary culture solution accommodated in the cell culture unit moves, and a second connection channel connected to the second connection channel, And a culture medium discarding section for culturing the cell culture medium.

The cell culture unit, the first connection channel, and the second connection channel may be one or at least two or more.

The first connection channel or the second connection channel may be provided with a plurality of protrusions protruding along the longitudinal direction or a plurality of protrusions protruding at intervals.

The height of the first connection channel may be higher than the height of the second connection channel.

The first connection channel may be provided with an inclined portion having a higher level of the culture liquid distributing portion side than the cell culturing portion side.

It is preferable that the cell culture units are partitioned by barrier ribs when a plurality of cell culture units are provided.

The culture liquid distributor, the first connection channel, and the second connection channel may share the culture liquid waste portion and be paired.

The culture liquid dispensing part and the culture liquid scavenging part may have a quadrangular shape or a circular shape in plan view.

Preferably, the culture liquid dispensing part is connected to a culture liquid supply part for supplying the cell culture liquid.

Preferably, the first connection channel is connected to a substance supply unit for supplying a chemical substance.

It is preferable that the culture liquid scavenging section is provided with a heating section for heating the lung culture fluid.

Preferably, a heat insulating material is provided between the cell culture unit and the culture liquid disposal unit to block heat supplied to the culture liquid disposal unit.

The multi-channel cell culture container is made of a synthetic resin material and is preferably formed by thermoforming or vacuum molding.

In this embodiment of the present invention, the cell culture liquid is supplied continuously or intermittently to the culture liquid distributing unit through the culture liquid supply unit, and the lung culture liquid is heated by the heating unit and evaporated to be discarded. Therefore, in the embodiment of the present invention, it is not necessary to perform a separate operation for exchanging the cell culture liquid, so that the experimenter can stably cultivate the cells for a long time and facilitate observation of the cells, thereby improving the reliability of the experiment.

In addition, embodiments of the present invention can supply different kinds of chemical substances to the cell culture unit through the substance supply unit or cultivate different kinds of cells in the cell culture unit while supplying the same kind of chemical substance. Thus, embodiments of the present invention can obtain independent observations of the effects of different kinds of chemicals on the same cells or independent observations of the effects of the same chemicals on different types of cells.

In addition, since the embodiment of the present invention includes a plurality of cell culture units, it is possible to obtain a large amount of experimental data under the same single experimental condition, thereby increasing the reliability of the experimental data by reducing the experimentation time and simultaneously performing the high- .

In addition, the embodiment of the present invention can be mass-produced by thermoforming or vacuum forming a synthetic resin material and can be used as a disposable material, thereby preventing contamination during cell experiments that may occur while reusing multi-channel cell culture containers .

1 is a perspective view showing a multi-channel cell culture container for explaining an embodiment of the present invention.
2 is a cross-sectional view taken along line II-II of FIG.
3 is a block diagram showing a main configuration of a multi-channel cell culture container for explaining an embodiment of the present invention.
4 is a perspective view showing another example of a multi-channel cell culture container according to an embodiment of the present invention.
5 is a perspective view showing still another example of a multi-channel cell culture container according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

1 is a perspective view for explaining an embodiment of the present invention. FIG. 2 is a cross-sectional view taken along line II-II of FIG. 1, and FIG. 3 is a cross- Respectively.

The multi-channel cell culture container of the embodiment of the present invention comprises a culture liquid distributing section 1, a first connecting channel 3, a cell culture section 5, a second connecting channel 7, and a culture liquid scavenging section 9 .

The culture liquid distributing section 1 may be a concave groove, and the multi-channel cell culture container may be rectangular in plan view. The culture liquid distributing section (1) is a space for accommodating a culture liquid capable of culturing cells.

3, the culture liquid supply section 11 can be connected to the culture liquid distributing section 1 such that the cell culture liquid is supplied to the culture liquid distributing section 1 side via a pipeline or the like. The culture medium supply section 11 can continuously supply, periodically, or intermittently supply the culture medium to the culture medium distribution section 1 for a predetermined period of time. A control part (not shown) is provided in the culture solution supply part 11 so that the culture solution can be supplied to the culture solution distribution part 1 by an amount required by the setting of the user.

The culture liquid distributing section 1 and the cell culture section 5 are separated into a partition wall and a first connection channel 3 may be provided on the partition wall.

The first connection channel 3 is a passage through which the cell culture solution accommodated in the culture solution distribution unit 1 flows and can be transmitted to the cell culture unit 5. The first connection channel (3) can be formed as a passageway having an open top portion. The first connection channel 3 may be provided with an inclined portion 3a. The inclined portion 3a provided in the first connection channel 3 may form the entirety of the first connection channel 3 and may be provided only partially on the cell culture portion 5 side. It is preferable that the inclined portion 3a of the first connection channel 3 is inclined at a low level on the side of the culture liquid distributing portion 1 and on the side of the cell culture portion 5. [ These first connection channels 3 can be arranged as many as the number of the cell culture units 5. [ A material supply unit 13 may be connected to the first connection channel 3. The substance supply part 13 can supply the biochemical material to the cell culture part 5. [ The end of the channel provided in the substance supply part 13 may be connected to or disposed on the upper side of the first connection channel 3 to supply the biochemical material to the first connection channel 3 via the first connection channel 3. [ have. The material supply unit 13 can control the supply amount of the biochemical material supplied to the first connection channel 3 by a separate control unit (not shown).

In the embodiment of the present invention, it is also possible to supply the biochemical material directly to the cell culture unit 5 through the substance supply unit 13. However, when the biochemical material is supplied to the cell culture unit 5 through the first connection channel 3, the biochemical material may be sufficiently diluted in the cell culture solution and sufficiently supplied in the cell culture unit 5 to be supplied. The biochemical material supplied to the cell culture unit 5 is an experimental material that reacts with the cells and affects the behavior of the cells.

The cell culture section 5 is partitioned into partition walls to form a plurality of grooves. The cell culture section 5 is a space in which the cells to be cultured (C, shown in FIG. 3) can be accommodated, and cell culture fluids and biochemical materials can also be accommodated. Each of the chambers of the cell culture section 5 is connected to the first connection channel 3 described above.

A second connection channel (7) is disposed between the cell culture section (5) and the culture liquid waste section (9).

The second connection channel 7 is a passage through which the pulmonary culture solution accommodated in the cell culture section 5 can be moved to the culture liquid waste section 9. It is preferable that the second connection channel 7 is provided by the number of the cell culture units 5.

It is preferable that the height h2 of the second connection channel 7 is lower than the height h1 of the first connection channel 3 based on the same bottom surface. That is, the height h1 of the first connection channel 3 is higher than the height h2 of the second connection channel 7. When the height h1 of the first connection channel 3 is greater than the height h1 of the second connection channel 7, Which is higher than the height h2 of the cell culture unit 7 in the cell culture unit 5, the cell culture solution moves to the cell culture unit 5 through the first connection channel 3 in the culture solution distribution unit 1, So that the accommodated lung fluid can be easily transferred to the culture liquid waste section 9 through the second connection channel 7. [

On the other hand, as shown in FIG. 4, the first connection channel 3 or the second connection channel 7 may be provided with a plurality of guides 3b along the longitudinal direction on the bottom surface thereof. The guide 3b is preferably disposed along the direction in which the culture liquid or the pulmonary culture fluid flows in the first connection channel 3 or the second connection channel 7 and is formed of wrinkles or has a predetermined height and protrudes in a long direction Lt; / RTI > The guide 3b may be formed in a wrinkled shape or a shape protruding in a long direction or may be formed so as to be smaller in height than the entire height of the first connection channel 3 and the second connection channel 7 And can protrude finely from the bottom surface.

The guide 3b is used to smoothly move the culture medium or the lung culture fluid due to the surface tension effect or the capillary effect when the culture medium or the pulmonary culture fluid moves along the first connection channel 3 or the second connection channel 7, It may be advantageous to control the flow of the lung culture fluid. In the embodiment of the present invention, the guide 3b is provided on the bottom surface of the first connection channel 3 or the second connection channel 7, but the present invention is not limited thereto, Or on the side of the wall surface as well as the bottom surface of the second connection channel 7. [

In the description of the embodiment of the present invention, the first connection channel 3 is shown and described in FIG. 4, but the present invention is not limited thereto and the second connection channel 7 may be applied in the same structure.

5 shows another example of the embodiment described in Fig. 4, in which a plurality of protrusions 3c are arranged at intervals on the bottom surface of the first connection channel 3 or the second connection channel 7. In Fig. The projection 3c, which is another example of the embodiment of the present invention, can also have the same or similar function as the guide 3b described above. The protruding portion 3c preferably has a small size and is preferably provided not higher than the height of the first connection channel 3 or the second connection channel 7 (the height of the portion where the culture liquid or the pulmonary culture fluid flows).

As described above, in the embodiment of the present invention, the guide 3b or the protrusion 3c is formed in the first connection channel 3 or the second connection channel 7 to maximize the surface tension effect and the capillary effect, It is shown that various structures can be implemented to smooth or control the flow.

The culture liquid waste portion 9 is connected to the second connection channel 7 and is a groove (or space) in which the pulp culture liquid can be received.

A heating section 15 may be provided on the outer bottom surface of the culture liquid waste section 9. The heating unit 15 provided on the side of the culture liquid scavenging unit 9 can serve to evaporate the lung fluid contained in the culture liquid scavenging unit 9. [ It is preferable that the heating section 15 heats the culture liquid waste section 9 only at a temperature enough to evaporate the pulmonary culture liquid.

The heating unit 15 is provided on the outer bottom surface of the culture liquid waste unit 9, but the present invention is not limited thereto. In some cases, the heating unit 15 is formed in a flat plate shape, .

On the other hand, it is preferable that a heat insulating material 17 is disposed between the cell culture part 5 and the culture liquid waste part 9. It is preferable that the heat insulating material 17 is disposed between the cell culture part 5 and the partition wall of the culture liquid waste part 9 (see Fig. 3). The heat insulating material 17 can prevent the heat generated by the heating unit 15 and the heat supplied to the culture liquid waste unit 9 from being transmitted to the cell culture unit 5. [ The cells cultured in the cell culture section 5 by the heat insulating material 17 can be cultured according to the experimental conditions set by the experimenter without being affected by the heat generated in the heating section 15.

The multi-channel cell culture container of this embodiment of the present invention can be mounted on a separate base (not shown), and the heating unit 15 and the heat insulating material 17 described above can be installed on a base (not shown) .

The multi-channel cell culture container of the embodiment of the present invention has a relatively thin thickness, and may be made of a biocompatible plastic material or a synthetic resin material, and may be manufactured by thermoforming or vacuum molding. Such an embodiment of the present invention has a structure suitable for mass production, and a multi-channel cell culture container can be used for disposable use.

In the description of the embodiment of the present invention, a plurality of first connection channels 3, cell culture units 5, and second connection channels 7 are provided. However, the present invention is not limited to this, One or more of the first connection channel 1, the first connection channel 3, the cell culture section 5, the second connection channel 7, and the culture liquid disposal section 9 may be formed.

Hereinafter, a process of culturing cells through the embodiment of the present invention will be described.

The cell culture liquid supplied from the culture liquid supply unit 11 in a state where the cells C are contained in the cell culture unit 5 flows into the culture liquid distribution unit 1. [ At this time, the cell culture liquid is supplied to the culture liquid distributor 1 continuously, periodically or intermittently under the control of a control unit (not shown). When the cell culture liquid is sufficiently filled in the culture liquid distributing section 1, the cell culture liquid that is further introduced moves to the cell culture section 5 along the first connection channel 3. The first connection channel 3 is made higher than the height of the cell culture section 5 so that the cell culture solution easily flows from the culture solution distribution section 1 to the cell culture section 5 along the slope section 3a.

In this state, the cells are cultured in the cell culture section 5 over time and the experimenter can easily observe from the outside.

When the biochemical material is supplied to the cell culture unit 5 through the material supply unit 13 according to need, the biochemical material is supplied to the cell culture unit 5 while being diluted in the cell culture liquid moving along the first connection channel 3 and diffused. Then, the cells in the cell culture section (5) are stimulated and influenced by the biochemical agent. Therefore, the observer can observe cells that respond to biochemicals.

Through this process, the lung culture fluid generated in the cell culture section 5 is overflowed and transferred to the culture liquid waste section 9 through the second connection channel 7.

At this time, since the temperature of the culture liquid waste part 9 is raised by the heating part 15, the waste liquid of the culture liquid waste part 9 is evaporated and discarded.

In the embodiment of the present invention, it is not necessary to withdraw the cell culture container from the experimental site in order to exchange the cell culture liquid through this process. Therefore, the embodiment of the present invention can increase the reliability of the experiment because the experimenter can stably perform long-time cell culture and cell observation.

Particularly, in the embodiment of the present invention, the different types of biochemical materials are supplied to the cell culture unit 5 through the substance supply unit 13 according to the experimental conditions, or the same type of biochemical materials are supplied to the cell culture unit 5, Lt; RTI ID = 0.0 > cells. ≪ / RTI > These embodiments of the present invention can obtain independent observations of the effects of different types of biochemicals on the same cells or independent observations of the effects of the same biochemical on different types of cells.

In the embodiment of the present invention, a lot of experimental data can be obtained under a single experimental condition, thereby reducing the experiment time and increasing the reliability of the experimental data. Further, the embodiment of the present invention is suitable for simultaneous high-speed inspection and mass inspection at the same time.

In addition, the embodiment of the present invention is capable of mass production and can be used as a disposable, so that it is possible to prevent contamination during cell experiments that may occur while reusing the multi-channel cell culture container.

FIG. 6 is a view for explaining another example of the embodiment of the present invention, showing a multi-channel cell culture container. The description of other examples of the embodiments of the present invention is the same as the description of the above-mentioned examples, and only the other parts will be described.

Another example of the embodiment of the present invention is that the culture liquid distributing section 1, the first connection channel 3, the cell culture section 5, and the second connection channel 7 share a culture liquid waste section 9, Are arranged in pairs. The embodiment of the present invention maximizes the number of the cell culture units 5, so that a large number of experimental data can be obtained in one experiment, and a large number of tests and a high-speed inspection are possible.

Figure 7 shows a multi-channel cell culture vessel as another example of an embodiment of the present invention. In the above description, the multi-channel cell culture container according to the embodiment of the present invention is shown in a rectangular shape. However, in another example of the embodiment of the present invention, the multi-channel cell culture container is circular in plan view. In particular, when the multi-channel cell culture container is formed in a circular shape as shown in another example of the embodiment of the present invention, the culture medium distribution part 1, the first connection channel 3, the cell culture part 5, 2 connection channel 7, and culture liquid waste section 9, but they may be disposed in the reverse order.

Another example of this embodiment of the present invention shows that the shape of the multi-channel cell culture container of the embodiment of the present invention can be variously configured.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, And it goes without saying that the invention belongs to the scope of the invention.

1. The culture solution dispensing part,
3. a first connection channel, 3a. Inclined portion, 3b. Guide, 3c. Protrusions,
5. Cell culture,
7. Second connection channel,
9. Culture medium waste,
11. The culture medium supply part,
13. Material supply,
15. Heating,
17. Insulation

Claims (13)

A culture fluid distribution section in which the cell culture fluid is accommodated,
A first connection channel connected to the culture solution dispenser to move the cell culture solution,
A cell culture unit connected to the first connection channel to receive the cell culture solution and cells to be cultured,
A second connection channel connected to the cell culture unit and through which the pulmonary culture solution accommodated in the cell culture unit moves, and
And a culture liquid scavenger unit connected to the second connection channel to receive a lung culture fluid,
The first connection channel or the second connection channel
A multi-channel cell culture container in which a plurality of protrusions protruding along the longitudinal direction are provided, or a plurality of protrusions protruding at intervals are provided. The method according to claim 1,
The cell culture unit, the first connection channel, and the second connection channel
One or at least two or more cells. delete The method according to claim 1,
The height of the first connection channel
Wherein the height of the second connection channel is higher than the height of the second connection channel. The method according to claim 1,
The first connection channel
Wherein the inclined portion is higher than the cell culture portion on the side of the culture solution distributing portion. The method according to claim 1,
The cell culture section
Wherein the plurality of cells are partitioned by barrier ribs. delete The method according to claim 1,
The culture solution dispensing part and the culture solution scavenger part
A multi-channel cell culture vessel comprising a square or a circle in plan view. The method according to claim 1,
The culture liquid distributing portion
And a culture solution supply part for supplying the cell culture solution is connected. The method according to claim 1,
In the first connection channel or cell culture section
A multi-channel cell culture vessel to which a substance supply part for supplying biochemical substances is connected. The method according to claim 1,
On the side of the culture liquid scavenger,
And a heating section for heating the pulmonary culture fluid is provided. The method according to claim 1,
Between the cell culture section and the culture liquid waste section
And a heat insulating material for shielding heat supplied to the culture liquid waste part is provided. The method according to claim 1,
The multi-channel cell culture vessel
A multi-channel cell culture vessel made of biocompatible plastic or synthetic resin material and manufactured by thermoforming or vacuum molding.
KR1020150116899A 2015-08-19 2015-08-19 Multichannel cell culture dish KR101706750B1 (en)

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JP2020028237A (en) * 2018-08-21 2020-02-27 ウシオ電機株式会社 Cell culture chip, and cell culture method using the same
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CN109943483A (en) * 2018-11-03 2019-06-28 宁波大学 Cell culture apparatus and method based on real-time complementary technology
KR102457496B1 (en) * 2021-10-15 2022-10-21 주식회사 에드믹바이오 Microfluidic device for mimicking biomimetic tissue with easy control of flow of fluid in channel
WO2023063660A1 (en) * 2021-10-15 2023-04-20 주식회사 에드믹바이오 Microfluidic device for biological tissue stimulation facilitating control of fluid flow inside channel

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