CN212955169U - Stem cell rolling culture tank - Google Patents
Stem cell rolling culture tank Download PDFInfo
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- CN212955169U CN212955169U CN202020740861.4U CN202020740861U CN212955169U CN 212955169 U CN212955169 U CN 212955169U CN 202020740861 U CN202020740861 U CN 202020740861U CN 212955169 U CN212955169 U CN 212955169U
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- stem cell
- culture tank
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Abstract
The utility model discloses a stem cell roll culture tank, including the tank body wall, the open end, the blind end, the feed liquor pipe, the drain pipe, stem cell access hole, back shaft, drive wheel, the arris of salient. The utility model provides a stem cell roll culture tank through reciprocal roll, has effectively reduced the damage of stirring rake shearing force to stem cell to promote the stem cell dispersion through the inboard abrupt edge of tank wall, gain fine cultivation effect.
Description
Technical Field
The utility model relates to a stem cell culture field especially relates to a stem cell rolls culture tank.
Background
The stem cells have a variety of types, and have been widely applied in many fields such as disease treatment, medical cosmetology, anti-aging, drug screening and the like. Stem cell culture has become a bottleneck for the development of the whole stem cell industry chain, and stem cell culture devices have become hot spots for the development of the stem cell culture field.
The current stem cell culture device is mainly from the microbial fermentation industry, and a culture tank is directly or simply adjusted for stem cell production. Because stem cells are not protected by cell walls like bacteria, the stirring paddle in the bacterial culture tank can cause great shearing force damage to the stem cells in culture, so that the stem cells die or stem cell variation is induced. The mild stirring mode is extremely important for stem cell production, and the development of a novel low-shearing-force stem cell culture tank is an urgent need for the development of the stem cell industry.
Disclosure of Invention
The utility model provides a stem cell roll culture tank, characterized by one can reciprocate the slow rolling closed tank body, and tank body wall inboard has the abrupt arris, and stem cell roll culture tank roll in-process can make the internal stem cell of jar obtain gentle stirring, effectively guarantees that the stem cell is abundant and the culture solution contact under the low shearing force condition, improves the culture effect.
The utility model discloses a concrete technical scheme is: add stem cell through stem cell access hole on the open end of stem cell roll culture tank, utilize the feed liquor pipe to provide nourishment and oxygen for stem cell, utilize the drain pipe discharge stem cell metabolic waste, at the cultivation in-process, when stem cell roll culture tank was reciprocal to be rolled, the protruding arris of cooperation tank body wall inboard made stem cell and culture solution fully contact, effectively absorbed nourishment and oxygen to waste material and carbon dioxide discharge. Finally, the stem cells are harvested through the stem cell access hole.
The stem cell inlet and outlet hole is used for inputting stem cells before culture and taking out the stem cells after culture: adjusting the stem cell access hole to the highest point when inputting stem cells before culturing; after culturing, when outputting stem cells, adjusting the inlet and outlet holes of the stem cells to the lowest point, and adding sterile air or sterile physiological saline through the liquid inlet pipe or the liquid outlet pipe to discharge all the stem cells in the stem cell rolling culture tank.
A filter membrane or filter element structure is arranged in a pipeline of the liquid inlet pipe or the liquid outlet pipe and external connecting equipment, so that the stem cells can be prevented from flowing out of the stem cell rolling culture tank; the pipeline can be used for operating liquid and can also be used for operating gas, and the operation is controlled by an externally connected valve; the in and out are for convenience of explanation only and can be freely switched in use.
The driving wheel of the stem cell rolling culture tank is assembled with the motor through the belt, and the motor can ensure that the stem cell rolling culture tank slowly rotates in a reciprocating mode within the range of 360 degrees, so that stem cells in the stem cell rolling culture tank are fully contacted with culture solution, and the exchange of feeding and waste is completed.
In order to effectively improve the culture control precision, various sensors such as temperature, pH value, dissolved oxygen and the like can be arranged at the opening end of the stem cell rolling culture tank, and stem cell growth regulation and control can be effectively completed by matching with auxiliary devices.
The beneficial effects of the utility model are that simple structure easily makes, excellent in use effect.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a stem cell rolling culture tank
FIG. 2 is a schematic view of the inner protruding edge structure of the wall of the tank body of the stem cell rolling culture tank
In the figure: the opening end 2 of the tank wall 1, the closed end 3, the supporting shaft 4, the driving wheel 4, the liquid inlet pipe 6, the liquid outlet pipe 7, the stem cell inlet hole 8 and the protruding edge 9 of the liquid outlet pipe 7
Detailed Description
Under the aseptic condition, the support shaft 4 of the stem cell rolling culture tank is assembled on the bracket of the culture box, the driving wheel 5 is tightly connected with the motor through a belt, and the liquid inlet pipe 6 and the liquid outlet pipe 7 are respectively connected with corresponding valves. The stem cell culture solution with a proper volume is input from the liquid inlet pipe 6 (prepared in advance and can meet the growth requirement of stem cells), the stem cell inlet-outlet hole 8 is rotated to a high position, the stem cells are opened under the aseptic condition and added into the stem cell rolling culture tank, then the stem cell inlet-outlet hole 8 is tightly closed, and the input and output of the stem cell culture solution (containing dissolved oxygen and carbon dioxide gas) are controlled by using the liquid inlet pipe 6 and the liquid outlet pipe 7, so that the growth requirement of the stem cells is ensured. And sampling through the stem cell access hole 8 for detection, and determining the stem cell culture end point. And connecting the stem cell access hole 8 with a stem cell conveying pipeline at a high position, turning the stem cell access hole 8 to a low position, and conveying the stem cells into a stem cell recovery container by using gas pressure to complete stem cell recovery.
In the process of rolling the stem cell rolling culture tank, the convex edges 9 on the inner side of the tank body wall 1 can ensure that the stem cells in the tank body are stirred gently, so that the stem cells are effectively ensured to be fully contacted with a culture solution under the condition of low shearing force, and the culture effect is improved.
The transmission wheel 5 is assembled with the motor through a belt, and the motor can ensure that the stem cell rolling culture tank slowly rotates in a reciprocating mode within the range of 360 degrees, so that stem cells in the stem cell rolling culture tank are fully contacted with culture solution, and the exchange of feeding and waste is completed.
Can set up various sensors on stem cell roll cultivation jar open end 2, accomplish the accurate control that stem cell cultivateed, if can effective control cultivation temperature through temperature sensor and the coupling of the accuse temperature device of cultivateing environment and culture solution, be connected through pH valve sensor and pH valve control device and can guarantee the stability of the pH valve of cultivateing the system, guarantee that stem cell has sufficient suitable oxygen supply through dissolved oxygen sensor and the coupling of control dissolved oxygen device. The various sensors may be directly mounted on the stem cell inlet/outlet hole 8, or may be previously installed at corresponding positions of the stem cell rolling culture tank as required.
The present embodiments are intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (6)
1. A stem cell rolling culture tank, characterized by comprising: the device comprises a tank body wall, an opening end, a closed end, a liquid inlet pipe, a liquid outlet pipe, stem cell inlet holes, a support shaft, a driving wheel and a protruding edge; the liquid inlet pipe, the liquid outlet pipe and the stem cell inlet and outlet holes are positioned at the opening end; the opening end and the closed end are provided with support shafts, and the driving wheel is positioned at the closed end; the protruding edge is positioned on the inner side of the wall of the tank body.
2. The stem cell rolling culture tank according to claim 1, wherein the stem cell rolling culture tank can roll back and forth within a range of 360 degrees.
3. The rolling stem cell culture pot of claim 1, wherein the ridges are formed on the inside of the wall of the pot to connect the open end and the closed end.
4. The rolling stem cell culture tank as set forth in claim 1, wherein the liquid inlet pipe and the liquid outlet pipe have inside them a strainer or a filter element.
5. A rolling stem cell culture pot according to claim 1 wherein the support shaft has two portions secured to the open end and the closed end respectively.
6. The rolling stem cell culture pot according to claim 1, wherein the support shaft is coaxial with the driving wheel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020740861.4U CN212955169U (en) | 2020-04-29 | 2020-04-29 | Stem cell rolling culture tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020740861.4U CN212955169U (en) | 2020-04-29 | 2020-04-29 | Stem cell rolling culture tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212955169U true CN212955169U (en) | 2021-04-13 |
Family
ID=75373977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020740861.4U Active CN212955169U (en) | 2020-04-29 | 2020-04-29 | Stem cell rolling culture tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212955169U (en) |
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2020
- 2020-04-29 CN CN202020740861.4U patent/CN212955169U/en active Active
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