CN110373309A - A kind of nucleic acid extraction amplification system and molecular detector arrangement - Google Patents
A kind of nucleic acid extraction amplification system and molecular detector arrangement Download PDFInfo
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- CN110373309A CN110373309A CN201910507406.1A CN201910507406A CN110373309A CN 110373309 A CN110373309 A CN 110373309A CN 201910507406 A CN201910507406 A CN 201910507406A CN 110373309 A CN110373309 A CN 110373309A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention discloses a kind of nucleic acid extraction amplification systems, including temperature control mechanism, pushing mechanism, magnetic mechanism, loading-unit and control unit；The side of the loading-unit is the temperature control mechanism for controlling the temperature in multiple loading storehouses respectively, and the other side is pushing mechanism；The pushing mechanism includes pushing pressing mechanism and pushing to obstruct mechanism；The promotion pressing mechanism is for getting through breakable seal layer and then being connected to adjacent loading storehouse；The mutual vibration of fluid for pushing barrier mechanism to be used to obstruct adjacent loading storehouse；The promotion pressing mechanism includes several baffles；Promotion barrier mechanism includes several push plates.And a kind of molecular detector arrangement, including shell, optical module and the nucleic acid extraction amplification system；The enclosure interior is equipped with chamber resettling, and the chamber resettling is used for the nucleic acid extraction amplification system；The optical module is used to carry out optical detection to substance in loading storehouse.The present invention has totally-enclosed, portable, integrated detection advantage.
Description
Technical field
The present invention relates to field of biological detection, more particularly, to a kind of nucleic acid extraction amplification system and detection device.
Background technique
In general, molecular diagnostic techniques include three nucleic acid extraction, amplification and detection steps.In the prior art for these three
Step is often that substep carries out.Firstly, be the extraction process of nucleic acid, in order to obtain accurate detection as a result, should during in it is right
Acquisition, storage and the transportation environment of sample have high requirement.Then, the extraction of nucleic acid, existing extractive technique packet are carried out
Include supercritical ultrasonics technology, freeze-thaw method, paramagnetic particle method etc..Currently used is paramagnetic particle method, is usually carried out by instrument for extracting nucleic acid.But due to nucleic acid
Process needed for extracting is complicated, and a series of processes such as cracking, purifying including sample take a long time, and the nucleic acid extracted is turning
Cross contamination is easy to produce during moving to PCR.Finally, carrying out Molecular Detection.Existing molecular diagnostic techniques, depend on
Polymerase chain reaction (PCR) expands nucleic acid molecules, is mostly completed by fluorescence quantitative PCR instrument.In amplification procedure, pass through
Fluorescent quantitation, obtains required various data, and by algorithm analyze to obtain it is qualitative or quantitative as a result, entire amplification and point
Analysis process needs a few hours that can complete.There is high requirement simultaneously for laboratory and operator.It is above-mentioned several to complete
A step, on the one hand, a possibility that required place space is very big, and the equipment being related to is more, increases cross contamination,
On the other hand, since equipment is more, step is more, trivial operations, thus the requirement for operator be also it is high, pre-job
It takes much time to give training and could really do actual molecules diagnostic work with after practice early period.
Such detection process, the period is long, complicated for operation, high to operator's requirement, very influence detection efficiency, pays
Labour cost it is also very much.Some prior arts to the simple flow of molecular diagnosis, reduction land occupation, lower the requirement etc. and to make
Improve, for example, application No. is a kind of 201611199449.0 collection nucleic acid extractions, expand, be detected on the special of integrated mechanical device
Sharp document uses the technological means such as sample cell, agent bin, hybridization slot, motor, barcode reader, sampling probe, realizes in core
Acid is extracted, is expanded, detecting integrated technical solution.Although the similar prior art realizes the integration of molecular diagnosis,
The problem of being there are still cross contamination, for example, using sampling probe mobile response liquid, waste liquid tank to the pollution of reagent the problems such as.Cause
This, improves in terms of realizing nucleic acid extraction, amplification, detection integration there is still a need for further.
Summary of the invention
The present invention requires operator high, processing effect for nucleic acid and PCR amplification process complexity is extracted in the prior art
Rate is low, sample technical problem easy to pollute, provides a kind of nucleic acid extraction amplification system and device.
The main object of the present invention are as follows: a kind of nucleic acid extraction amplification system is provided.
Another object of the present invention is: a kind of molecular detector arrangement is provided, to realize the portable nucleic acid extraction of integration
With expand and the nucleic acid of amplification is detected.
It is complicated in order to solve to extract nucleic acid and PCR amplification process in the prior art, high, treatment effeciency is required operator
It is low, the sample technical problem present invention easy to pollute:
A kind of nucleic acid extraction amplification system, including temperature control mechanism, pushing mechanism, magnetic mechanism, loading-unit and control are provided
Unit processed.The loading-unit includes carrier cavities, and the inside of the carrier cavities is equipped with multiple breakable seal layers, by institute
It states carrier cavities and is divided into multiple sequentially connected, mutually independent loading storehouses.The top of the carrier cavities is equipped with salable
Sample injection port.The side of the loading-unit is the temperature control mechanism for controlling the temperature in multiple loading storehouses respectively,
The other side is pushing mechanism.The pushing mechanism includes pushing pressing mechanism and pushing to obstruct mechanism；The promotion pressing mechanism
For getting through breakable seal layer and then being connected to adjacent loading storehouse；The promotion barrier mechanism is for obstructing adjacent loading storehouse
The mutual vibration of fluid.The promotion pressing mechanism includes several baffles；Promotion barrier mechanism includes several push plates；The gear
Plate and the push plate are separately connected first driving device.The baffle be closely located to the breakable seal layer can barrier fluid
Mutual vibration, the push plate are located proximate to the loading storehouse and can break the breakable seal layer by fashion of extrusion and realize phase
The flowing of reagent between the adjacent loading storehouse；The face being extruded in the loading storehouse is flexible structure, conducive to squeezing for the push plate
The progress of pressure.The magnetic mechanism is used for the magnetic bead that is sucked in the loading storehouse and the magnetic bead can be driven to be moved to each loading
The corresponding position in storehouse.Described control unit is electrically connected in the temperature control mechanism, pushing mechanism and magnetic mechanism.
General principles of the invention are that the pre- breakable seal layer that first passes through divides different reaction reagents in different reactions
Then storehouse realizes that the reagent in adjacent loading storehouse mixes by breaking breakable seal layer, the waste liquid after reaction is obstructed by baffle,
Cross contamination caused by crossfire is prevented, while carrying out the extraction of nucleic acid using paramagnetic particle method；Carry out the amplification of nucleic acid again later.Entirely
System is completely enclosed, and there is no the risks of cross contamination.
Firstly, the present invention carries out the processes such as sample process, nucleic acid extraction, amplification using loading storehouse, required reagent is pre-
It is encapsulated in inside loading storehouse, gets through the interval between adjacent loading storehouse in conjunction with pushing mechanism, while controlling respectively using temperature control mechanism
The temperature in each loading storehouse is made, the disposable integration of entire reaction process is completed, and has reached sample into the effect as a result gone out.This
Outside, unlike traditional equipment need substep detected, to save pilot process, greatly improve detection efficiency, entirely
Process is 15~30 minutes.
Pushing mechanism, temperature control mechanism, magnetic mechanism and loading-unit of the present invention can accomplish smaller body
Product, so land occupation very little.Compared to conventional molecular diagnostic device, great portability.
Loading storehouse can be sealed in the form of sealing cover in the present invention, and sealing cover can be set in inlet, with load
The connection type of the main part in object storehouse can be screw-threaded coupling, snap connection, hat type cooperation, preferably with screw-threaded coupling.This
The structure of sample not only acts as sealing function of the pre-filled reagent in preservation and transportational process, and guarantees that entire reaction process is carrying
It is completed in object storehouse, accomplishes totally-enclosed effect.The main structure material in loading storehouse is flexible high molecular material, and surface heatproof is not less than
100 DEG C, while there is certain elasticity and toughness, can have under specific circumstances certain dilatancy and will not rupture.It carries
The structure of object unit includes packed or oval tubular, and the outside of loading-unit can be equipped with bracket, be used to support flexible loading list
Member.Bracket can also be used cooperatively with sheath, further increase the leak-proof energy of loading-unit.The shape of sheath includes
The form of polygon sleeve, wall thickness >=0.2mm can be directly sleeved on loading-unit, and be consolidated by way of tight fit or detent
The relative position of fixed itself and loading-unit.Loading-unit includes multiple loading storehouses, and the volume in each loading storehouse is not more than 2ml.It carries
Object unit includes sample entrance port, and material is made of high molecular material, is fitted close with sealing cover, prevents leakage gas leakage.Sample
Entrance is connected to loading storehouse, and type of attachment includes glue bonding, ultrasonic bonding.
As a preferred solution of the present invention, the magnetic mechanism includes magnetic part and for driving the magnetic substance to be moved to
Second driving device of each loading bin location；The magnetic part is close to the side in the loading storehouse, second driving device
The movement of the magnetic substance during for realizing nucleic acid extraction and amplification.
As more preferable scheme of the invention, second driving device is equipped with sliding block, and the sliding block connects the magnetic
Suction portion.
Magnetic part is used for the magnetic bead fixed in loading storehouse, by pushing the promotion of pressing mechanism to realize reagent in loading storehouse
Movement repeatedly；Magnetic part is the object that can generate magnetic force, preferably magnet, and the shape of magnet can be any polygon
Shape, it is preferred that can be hexahedron or cylinder.Magnetic part is fixed on sliding block, can be fixed on by way of buckling or being bonded
On sliding block, and it is not limited to the fixed form for buckling or being bonded, as long as can achieve the effect that fix magnetic part on sliding block.
When reagent handles the loading storehouse in different location, driving device drives slide block movement, realizes inside the loading storehouse of different location
Magnetic bead fixation.
As a preferred solution of the present invention, the magnetic mechanism further includes magnetic threaded rod, the magnetic threaded rod screw thread
It is socketed the sliding block, second driving device is motor, and the motor connects the magnetic threaded rod.Here magnetic is given
A kind of specific connection type of mechanism, still, however it is not limited to which the form combination of motor, threaded rod can be realized the fortune of magnetic part
Dynamic and positioning.
As a preferred solution of the present invention, the first driving device includes motor, and the baffle connects the motor, institute
It states push plate and connects the motor, the motor can individually control the baffle and the push plate；The baffle resists institute
Breakable seal layer is stated, the push plate resists the loading storehouse；The quantity of the baffle, the push plate and the motor with it is described
The quantity of loading storehouse and the breakable seal layer is corresponding.Here a kind of conventional way of propelling is given, it is clear that as long as energy
Reach and squeeze loading storehouse, realize that the mechanism for the connection for getting through adjacent loading storehouse is all possible, such as crankshaft connection or threaded rod
Pushing mechanism.Motor in this programme equally can be specially direct current generator, stepper motor, servo motor, can be according to need
It wants, different motors is installed, it is clear that thrust can be generated to pushing mechanism and the effects of acceleration and deceleration, and control bit as desired
It sets or the motor of corner is all possible.In order to reach control effect, the quantity of motor is multiple, it is preferred that it is 21, it can be with
Match the use with the loading-unit in 11 loading storehouses.Quantity, the quantity of push plate and the quantity in loading storehouse of baffle are also phase
It is corresponding.Baffle and push plate driving method are not limited to threaded rod driving, can be realized the promotion of baffle so as to stop to try
Agent is flowed through from the position, can be realized the structure of effect that push plate pushes the reagent in agent bin axially to move repeatedly be all can be with
, such as connecting rod connection or guide rod connection or spring combination guide rod or threaded rod.
As a preferred solution of the present invention, the baffle includes first end and bracket；The end is elastic ends, institute
Stating bracket is rigid stent.The material of rigid stent can be high molecular material or metal material, it is clear that have enough supports
The material of intensity is all possible.Soft end can be macromolecular material, including TPU, silica gel.In addition, the length of end
Degree can make according to the width in loading storehouse, it is preferred that its length range is 1~50mm.Also, end need not be all soft
, such as structure is to wrap up the end that outer layer is elastic material.
As a preferred solution of the present invention, the push plate includes the second end, push plate heating film and push plate seat；Described second
End is fixedly connected with push plate heating film；The push plate heating film is fixedly connected with push plate seat.The structure of push plate is not limited to the second end
The connection type being fixed on push plate seat, it is all to be capable of fixing the second end, it can promote and there is enough intensity
Connection type.The shape of the second end includes hexahedron, cylindrical body, preferably hexahedron.Because the second end also has
Play the role of conducting heat, so, material needs to select high temperature resistant or metal material, it is preferred that selection metal material.
As a preferred solution of the present invention, the temperature conditioning unit includes heated support plate, heating plate, heater element and temperature
Sensor；The heater element and the temperature sensor are respectively fixedly connected with the heating plate；The heating plate is fixedly connected
In the heated support plate.
As a preferred solution of the present invention, the heater element and the heating plate using clamping or it is bonding by the way of fix
Connection；The temperature sensor and the heating plate using clamping or it is bonding by the way of be fixedly connected.Heating plate can be using not
The material of the good heat conductivities such as rust steel, aluminium alloy or copper is made, and is preferably selected brass.The quantity of heating plate can determine according to demand
System, best quantity are 10 or 11.
As a preferred solution of the present invention, the heater element is the group of heating film or semiconductor or heating film and semiconductor
It closes.The minimum number of heater element is 1, preferably 11, can achieve the effect of preferable control temperature.Heater element
Shape can be round or arbitrary polygon, preferably quadrangle.
As a preferred solution of the present invention, the semiconductor is TEC semiconductor.
In order to realize the portable nucleic acid extraction of integration and amplification and detect to the nucleic acid of amplification, of the invention is another
A purpose is:
A kind of molecular detector arrangement, including shell, optical module and molecular detection system are provided；The enclosure interior is equipped with
Chamber resettling, the chamber resettling is for carrying above-mentioned nucleic acid extraction amplification system；The optical module is used for in loading storehouse
Substance carries out optical detection.
The loading storehouse that the present invention uses is always maintained at full closeding state during processing, pollution-free with external environment, whole
A reaction process realizes totally-enclosed.In actual use, entire reaction system is inside apparatus casing, either shell with
Between loading storehouse or between shell and external environment, do not pollute, it is good to accurately providing for sample detection result
Environment.
The present invention diagnoses the operation format and system for reagent, equipment and each process complexity relative to conventional molecular, only
2 to 3 minutes artificial sample-addings are needed, remaining process is completed by system.It reduces and operator is required, without to operator
Carry out long-term complicated training.
The advantage that the present invention is limited with no place, specific manifestation are as follows: it is easy to carry since system is compact and flexible, it compares
It can only be carried out in laboratory in conventional molecular diagnosis, this system allows operator according to project needs are examined, and is testing
Other places other than room work.
The advantage that the present invention has expansibility good, be in particular in: the flexibility of system determines that equipment of the same race can be right
Different detection projects is answered, thus the very advantageous in opening.It is applicable not only to hospital clinical department and inspection center carries out
Quickly detection, is also applied for the related personnel of scientific research institution or Molecular Detection field, in the detection project of the platform development oneself.
As a preferred solution of the present invention, the optical module is set to the ambient side in the loading storehouse.
As a preferred solution of the present invention, the heater element of the heating region and corresponding position is equipped with through-hole,
The side of the through-hole is equipped with the optical module.
As a preferred solution of the present invention, the chamber resettling is sealable chamber, further improves molecule processing
The leakproofness of environment, main purpose are that sample is improved detection success rate by extraneous interference in order to prevent.
As a preferred solution of the present invention, the chamber resettling passes through lidstock；The capping is set to the shell
On.
As a preferred solution of the present invention, described to cover the hinged or shell that is slidably connected.
As a preferred solution of the present invention, the shape of the through-hole be round or polygon any one.
As a preferred solution of the present invention, the quantity of the through-hole is at least 1.
As a preferred solution of the present invention, the optical module includes excitation-light unit and optical detection apparatus.Exciting light
Device includes light-emitting component, lens, exciter filter and excitation dichroscope.Wherein, lens is are selectable elements, for converging
Poly- exciting light, quantity is determined by the light intensity of excitation light source, if light intensity converges enough, can choose without using lens.Its
In, exciter filter is used to filter the light wave of inessential wave band in exciting light, and quantity is at least 1, and specific number is according to excitation light source
To determine.The wave band and excitation light source wave band of exciter filter correspond.The shape that excitation filters includes cylinder, hexahedron,
Size can be not quite similar according to optical path size.Dichroscope is excited, reflection and transmission band are according to each excitation light source wave
Section is not quite similar, and for the transmission of specific band light in excitation light path, it is made to irradiate object under test；Its shape includes cylinder, six
Face body, size are arranged according to optical path size；Its type includes reflecting mirror, two to trichromscope, two to Multicolour mirror.Optical detection
Device includes detecting element, and lens detect optical filter, detects dichroscope.
As a preferred solution of the present invention, the excitation-light unit is LED light or black lamp or incandescent lamp.Wherein LED light
Quantity is at least 1, it is preferable to have the LED light of 2 corresponding different-wavebands.
As a preferred solution of the present invention, the wave band of the LED light includes FAX, HEX, ROX and CY5.
As a preferred solution of the present invention, the optical detection apparatus includes photodiode PD or photomultiplier tube PMT.
As a preferred solution of the present invention, the molecular detector arrangement further includes the display and electricity on shell
Source.Display its can be touch screen.Power supply is external including being built in machine or independence with equipment type of attachment.
As a preferred solution of the present invention, the molecular detector arrangement further includes code reader, and the code reader is set to institute
It states on the side wall of shell, the loading-unit is equipped with the barcode scanning region to match with the code reader.The loading-unit can
To add protective case, if adding protective case, barcode scanning region is arranged in accordingly on the protective case.
As a preferred solution of the present invention, the barcode scanning region setting two dimensional code or bar code.
Control unit in the present invention includes main control module, temperature control module, liquid treatment module, optical module, display mould
Block, power module；The temperature control module, the liquid treatment module, the optical module, the display module, the power supply
The main control module is electrically connected in module；The temperature control module is electrically connected the temperature control mechanism；The optical module packet
Include the excitation-light unit and optical detection apparatus of electric connection；The liquid treatment module is electrically connected the pushing mechanism；Institute
It states liquid treatment module and is electrically connected the magnetic mechanism；The display module is electrically connected the display；The power supply mould
Block is electrically connected power supply；The barcode scanning module is electrically connected code reader.
As a preferred solution of the present invention, detection of nucleic acids control system further includes barcode scanning module；The barcode scanning module is electrical
Connect the main control module.Communication module includes common the leading to of any one or more combination such as WiFi, bluetooth, network interface, GPS
News mode can be selected according to demand.
As a preferred solution of the present invention, detection of nucleic acids control system further includes communication module；The communication module is electrical
Connect the main control module.
Molecular detector arrangement in the present invention can be used for field include clinical department or ICU or emergency treatment or Disease Control and Prevention Center or
Food safety or scientific research or criminal investigation or family or military affairs or the quarantine of drugs or customs inspection or pet or aquatic products or water quality or forestry
Or the molecular diagnosis of animal husbandry.
The applicable reagent of nucleic acid extraction amplification system of the present invention includes dry state or liquid reagent.Specifically include lysate, egg
White enzyme k, buffer, PCR premixed liquid.
The condition of the applicable preservation of reagent of the invention or transport is -20 DEG C~30 DEG C.
Compared with prior art, the beneficial effects of the present invention are:
The present invention carries out sample process, nucleic acid extraction, the processes such as amplification using loading storehouse, by required reagent it is pre-packaged
Inside loading storehouse, the interval between adjacent loading storehouse is got through in conjunction with pushing mechanism, while being controlled respectively using temperature control mechanism each
The temperature in loading storehouse, the disposable integration of entire reaction process are completed, and have reached sample into the effect as a result gone out.In addition, unlike
Traditional equipment needs substep to be detected, to save pilot process, greatly improves detection efficiency, whole process 15
~30 minutes.
The loading storehouse that the present invention uses is always maintained at full closeding state during processing, pollution-free with external environment, whole
A reaction process realizes totally-enclosed.In actual use, entire reaction system is inside apparatus casing, either shell with
Between loading storehouse or between shell and external environment, do not pollute, it is good to accurately providing for sample detection result
Environment.
Pushing mechanism, temperature control mechanism, magnetic mechanism and loading-unit of the present invention can accomplish smaller body
Product, so, take up an area very little.Such as in geometric dimension be 170mm × 200mm × 230mm, it can be complete in weight≤4kg shell
At all processes of detection.Compared to conventional molecular diagnostic device, great portability.
The present invention diagnoses the operation format and system for reagent, equipment and each process complexity relative to conventional molecular, only
2 to 3 minutes artificial sample-addings are needed, remaining process is completed by system.It reduces and operator is required, without to operator
Carry out long-term complicated training.
The advantage that the present invention is limited with no place, specific manifestation are as follows: it is easy to carry since system is compact and flexible, it compares
It can only be carried out in laboratory in conventional molecular diagnosis, this system allows operator according to project needs are examined, and is testing
Other places other than room work.
The advantage that the present invention has expansibility good, be in particular in: the flexibility of system determines that equipment of the same race can be right
Different detection projects is answered, thus the very advantageous in opening.It is applicable not only to hospital clinical department and inspection center carries out
Quickly detection, is also applied for the related personnel of scientific research institution or Molecular Detection field, in the detection project of the platform development oneself.
Detailed description of the invention
Fig. 1 is nucleic acid extraction amplification system schematic view of the front view of the invention.
Fig. 2 is the side structure schematic view of nucleic acid extraction amplification system of the invention.
Fig. 3 is the side elevational cross-section structural schematic diagram of nucleic acid extraction amplification system of the invention.
Fig. 4 is partial enlargement structural representation at A in Fig. 3.
Fig. 5 is the overlooking structure diagram of nucleic acid extraction amplification system of the present invention.
Fig. 6 is the perspective view of nucleic acid extraction amplification system of the present invention.
Fig. 7 is the protective cover structure diagram of nucleic acid extraction amplification system of the present invention.
Fig. 8 is the structural schematic diagram of the portable molecular detector arrangement of Full enclosed integrated of the invention.
Fig. 9 is the longitudinal profile structure schematic of the portable molecular detector arrangement of Full enclosed integrated of the invention.
Figure 10 is the schematic perspective view of the portable molecular detector arrangement of Full enclosed integrated of the invention.
Figure 11 is optical module structure schematic diagram.
Figure 12 is optical module schematic illustration.
Figure 13 is molecular detection system structural schematic diagram of the invention.
In figure: 1, shell；11, display；12, power supply；13, code reader；2, chamber resettling；21, it covers；3, loading list
Member；31, loading storehouse, 32, breakable seal layer；33, barcode scanning region；34, bracket；35, protective case；4, temperature control mechanism；41, it heats
Support plate；42, heating plate；43, heater element；44, temperature sensor；45, through-hole；5, pushing mechanism；51, baffle；511,
One end；512, bracket；52, push plate；521, the second end；522, push plate heating film；523, push plate seat；53, the first driving dress
It sets；54, threaded rod is promoted；6, magnetic mechanism；61, magnetic part；62, sliding block；63, the second driving device；64, magnetic threaded rod；
7, optical module；71, excitation-light unit；72, optical detection apparatus；7111, light-emitting component；7112, lens；7113, excitation filter
Mating plate；7114, dichroscope is excited；7221, detecting element；7222, lens；7223, optical filter is detected；7224, detection two to
Look mirror.
Specific embodiment
The present invention is further illustrated With reference to embodiment.
The same or similar label correspond to the same or similar components in the attached drawing of the embodiment of the present invention；It is retouched in of the invention
In stating, it is to be understood that if there is the orientation of the instructions such as term " on ", "lower", "left", "right", "top", "bottom", "inner", "outside"
Or positional relationship is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of description of the present invention and simplification of the description, and
It is not that the device of indication or suggestion meaning or element must have a particular orientation, be constructed and operated in a specific orientation, therefore
The terms describing the positional relationship in the drawings are only for illustration, should not be understood as the limitation to this patent.
In addition, if there is the terms such as " first ", " second " to be used for description purposes only, be mainly used for distinguishing different devices,
Element or component (specific type and construction may identical may also be different), is not intended to show or implies indicated fill
It sets, the relative importance and quantity of element or component, and should not be understood as indicating or implying relative importance.
Embodiment 1
As shown in figures 1 to 6, the present embodiment provides a kind of nucleic acid extraction amplification system, including temperature control mechanism 4, pushing mechanism 5,
Magnetic mechanism 6, loading-unit 3 and control unit.Loading-unit 3 includes carrier cavities, the inside of carrier cavities be equipped with it is multiple can
Sealant 32 is destroyed, carrier cavities are divided into multiple sequentially connected, mutually independent loading storehouses 31.The top of carrier cavities
End is equipped with sealable sample injection port.The side of loading-unit 3 is the control for controlling the temperature in multiple loading storehouses 31 respectively
Warm mechanism 4, the other side are pushing mechanism 5.Pushing mechanism 5 includes pushing pressing mechanism and pushing to obstruct mechanism；Push extruder
Structure is for getting through breakable seal layer and then being connected to adjacent loading storehouse 31；Push barrier mechanism for obstructing adjacent loading storehouse 31
The mutual vibration of fluid.Pushing pressing mechanism includes several baffles 51；Pushing barrier mechanism includes several push plates 52；51 He of baffle
Push plate 52 is separately connected first driving device 53.Baffle 51 be closely located to breakable seal layer 32 can barrier fluid mutually go here and there
It is dynamic, push plate 52 be located proximate to loading storehouse 31 can be broken by fashion of extrusion breakable seal layer 32 realize adjacent loading storehouse 31 it
Between reagent flowing；The face being extruded in loading storehouse 31 is flexible structure, conducive to the progress of the extruding of push plate 52.Magnetic mechanism 6
Magnetic bead for being sucked in loading storehouse 31 simultaneously can drive magnetic bead to be moved to the corresponding position in each loading storehouse 31.Temperature control mechanism 4 pushes away
Control unit is electrically connected in motivation structure 5 and magnetic mechanism 6.
As a preferred solution of the present invention, magnetic mechanism 6 is including magnetic part 61 and for driving magnetic bead to be moved to each load
Second driving device 63 of 31 position of object storehouse；Magnetic part 61 close to the side in loading storehouse 31, the second driving device 63 for realizing
The movement of magnetic bead during nucleic acid extraction and amplification.Second driving device 63 is equipped with sliding block 62, and sliding block 62 connects magnetic part
61.Magnetic part 61 is used for the magnetic bead fixed in loading storehouse 31, is tried by pushing the promotion of pressing mechanism to realize in loading storehouse 31
The reciprocating motion of agent；Magnetic part 61 is the object that can generate magnetic force, such as magnet.The shape of magnet can be arbitrary polygon,
It preferably, can be hexahedron or cylinder.Magnetic part 61 is fixed on sliding block 62, can be fixed on by way of buckling or being bonded
On sliding block 62, and it is not limited to the fixed form for buckling or being bonded, as long as the effect for fixing magnetic part 61 on sliding block 62 can be reached
Fruit.When reagent handles the loading storehouse 31 in different location, the second driving device 63 is moved with movable slider 62, is realized different
The fixation of magnetic bead inside the loading storehouse 31 of position.Magnetic mechanism 6 further includes magnetic threaded rod 64,64 thread bush of magnetic threaded rod
Sliding block 62 is connect, the second driving device 63 is motor, and motor connects magnetic threaded rod 64.Here one kind of magnetic mechanism 6 is given
Specific connection type, still, however it is not limited to which the form combination of motor, threaded rod, the movement and positioning that can be realized magnetic part are i.e.
It can.
First driving device 53 includes motor, and motor connects baffle 51 and push plate 52, and can individually control baffle
51 and push plate 52.Baffle 51 resists breakable seal layer 32, and push plate 52 resists loading storehouse 31；Baffle 51, push plate 52 and motor
Quantity is corresponding with the quantity in loading storehouse 31 and breakable seal layer 32.Here a kind of conventional way of propelling is given, it is clear that
Loading storehouse is squeezed as long as can reach, realizes that the mechanism for the connection for getting through adjacent loading storehouse 31 is all possible, such as crankshaft connection
Or the pushing mechanism of threaded rod.Motor in this programme equally can be specially direct current generator, stepper motor, servo motor, can
With as needed, different motors is installed, it is clear that the effects of thrust and acceleration and deceleration capable of being generated to pushing mechanism, and according to needing
Seeking the motor of control position or corner is all possible.In order to reach control effect, the quantity of motor is multiple, it is preferred that is
21, the use of the loading-unit with 11 loading storehouses can be matched.The quantity of baffle 51, the quantity of push plate 52 and loading storehouse
31 quantity is also corresponding.52 driving method of baffle 51 and push plate is not limited to threaded rod driving, can be realized baffle 51
It pushes so as to which reagent is stopped to flow through from the position, the reagent that can be realized in the promotion agent bin of push plate 52 is axially transported repeatedly
The structure of dynamic effect is all possible, such as connecting rod connection or guide rod connection or spring combination guide rod or threaded rod.
Baffle 51 includes first end 511 and bracket 512；First end 511 is elastic ends, and bracket 512 is hard branch
Frame.The material of rigid stent can be high molecular material or metal material, it is clear that having the material of enough support strengths is all
Can with.Elastic ends can be macromolecular material, including TPU, silica gel.In addition, the length of elastic ends can basis
The width in loading storehouse makes, it is preferred that its length range is 1~50mm.Also, first end need not be all soft, example
If structure is to wrap up the end that outer layer is elastic material.Push plate 52 includes the second end 521, push plate heating film 522 and push plate seat
523；The second end 521 is fixedly connected with push plate heating film 522；Push plate heating film 522 is fixedly connected with push plate seat 523.Push plate 52
Structure is not limited to the connection type that the second end 521 is fixed on push plate seat 523, all to be capable of fixing the second end 521, makes it
It can promote and have the connection type of enough intensity.The shape of the second end 521 includes hexahedron, cylindrical body, excellent
Choosing is hexahedron.Because the second end also has the function of conducting heat, material needs to select high temperature resistant or metal
Material, it is preferred that selection metal material.
Temperature control mechanism 4 includes heated support plate 41, heating plate 42, heater element 43 and temperature sensor 44；Heater element
43 are respectively fixedly connected with heating plate 42 with temperature sensor 44；Heating plate 42 is fixedly connected on heated support plate 41.Heater element
43 with heating plate 42 using clamping or it is bonding by the way of be fixedly connected；Temperature sensor 44 and heating plate 42 are using clamping or bonding
Mode be fixedly connected.Heating plate 42 can be made of the material of the good heat conductivities such as stainless steel, aluminium alloy or copper, preferably be selected
Select brass.The quantity of heating plate 42 can customize according to demand, and best quantity is 10 or 11.Heater element 43 is heating film
Or the combination of semiconductor or heating film and semiconductor.The minimum number of heater element 43 is 1, preferably 11, be can achieve
The effect of preferable control temperature.The shape of heater element 43 can be round or arbitrary polygon, preferably quadrangle.Partly lead
Body is TEC semiconductor.
The control unit of the present embodiment as shown in figs. 10-12, including main control module, temperature control module, liquid treatment module, light
Learn module, display module, power module；Temperature control module, optical module, display module, power module, divides liquid treatment module
It electricity Xing Lianjie not main control module；Temperature control module is electrically connected temperature control mechanism；Optical module includes the excitation-light unit being electrically connected
And optical detection apparatus；Liquid treatment module is electrically connected pushing mechanism；Liquid treatment module is electrically connected magnetic mechanism；Display
Module is electrically connected display；Power module is electrically connected power supply.Barcode scanning module is electrically connected main control module, can read two dimension
Perhaps the above-mentioned two dimensional code of bar code or bar code record corresponding detection project information to code.Communication module is electrically connected master control
Module, communication module include the common communication modes of any one or more combination such as WiFi, bluetooth, network interface, GPS, can be with
It is selected according to demand.
Embodiment 2
As is seen in figs 7-10, the molecular detector arrangement in the present embodiment, including shell 1, optical module 7 and Molecular Detection system
System；Chamber resettling 2 is equipped with inside shell 1, chamber resettling 2 is for carrying above-mentioned nucleic acid extraction amplification system；Optical module 7 is used for
Optical detection is carried out to substance in loading storehouse 31.
Chamber resettling 2 is sealable chamber, further improves the leakproofness of molecule processing environment, main purpose be for
It prevents sample by extraneous interference, improves detection success rate.Chamber resettling 2 passes through 21 sealing of capping；Capping 21 is set to
On shell 1.As a preferred mode, capping 21 is hinged or is slidably connected to shell.As a preferred mode, shell
1 is additionally provided with display 11, can be touch screen.Power supply 12 is external including being built in machine or independence with equipment type of attachment.
In addition, being additionally provided with code reader 13 on shell 1, loading-unit 3 is equipped with the barcode scanning region 33 to match with code reader, barcode scanning region
33 also can be set on protective case 35, as a preferred embodiment, barcode scanning region 33 is arranged on protective case 35.It sweeps
Two dimensional code or bar code is arranged in code region 33.In the present embodiment, two dimensional code is arranged in barcode scanning region 33.
The shape of through-hole 45 be round or polygon any one；The quantity of through-hole 45 is at least 1.Heater element 43 is
The combination of heating film or semiconductor or heating film and semiconductor.In the present embodiment, semiconductor is TEC semiconductor.
Optical module 7 includes excitation-light unit 71 and optical detection apparatus 72.Excitation-light unit 71 includes light-emitting component
7111, lens 7112, exciter filter 7113 and excitation dichroscope 7114.Wherein, lens 7112 are used to be selectable elements
In convergence exciting light, quantity is determined by the light intensity of excitation light source, if light intensity converges enough, can choose without using saturating
Mirror.Wherein, exciter filter 7113 is used to filter the light wave of inessential wave band in exciting light, and quantity is at least 1, specifically counts basis
Excitation light source determines.The wave band and excitation light source wave band of exciter filter correspond.Excitation filter shape include cylinder,
Hexahedron, size can be not quite similar according to optical path size.Dichroscope 7114 is excited, reflection and transmission band are according to each
Excitation light source wave band is not quite similar, and for the transmission of specific band light in excitation light path, it is made to irradiate object under test；Its shape packet
Cylinder, hexahedron are included, size is arranged according to optical path size；Its type includes reflecting mirror, two to trichromscope, two to polychrome
Mirror.Optical detection apparatus includes detecting element 7221, and lens 7222 detect optical filter 7223, detects dichroscope 7224.According to
The portable molecular detector arrangement of the Full enclosed integrated of claim 14, excitation-light unit are LED light or black lamp or incandescent lamp.Its
The quantity of middle LED light is at least 1, it is preferable to have the LED light of 2 corresponding different-wavebands.The wave band of LED light include FAX, HEX,
ROX and CY5.Optical detection apparatus includes photodiode PD or photomultiplier tube PMT.Both elements are preferably photoelectricity two
Pole pipe PD.
Specific working mode of the invention are as follows:
For extracting nucleic acid in blood sample and carrying out PCR detection:
The pre-packaged various reaction reagents in loading storehouse；Loading-unit 3 includes 10 sections of loading storehouses, and each section by number 31- (1-
10) it represents；Wherein the length of loading storehouse 31-1 is longer, about 2 times of remaining each loading storehouse length, the length in remaining each loading storehouse
It is equal；Each loading storehouse is separated by breakable seal layer 32.
Contain pre-packaged reagent in each loading storehouse: 31-1 is sample storage area, can receive blood sample；Encapsulation in 31-2
80 μ L of buffer；80 μ L of draining in being encapsulated in 31-3；80 μ L protease k are encapsulated in 31-4；80 μ L of magnetic-particle is encapsulated in 31-5；
80 μ L of lysate is encapsulated in 31-6；80 μ L of cleaning solution is encapsulated in 31-7；80 μ L of eluent is encapsulated in 31-8；50 μ L are encapsulated in 31-9
PCR premixed liquid 1；The PCR premixed liquid 2 of 50 μ L of encapsulation in 31-10.
The present invention contains extraction, purifying and PCR reaction and analysis for nucleic acid, and all steps are incorporated into one
In the nucleic acid extraction amplification system of change, whole process realizes complete closure processing, be contaminated molecular diagnosis environment will not.
Specific step is as follows:
Sample-adding by hand.Sealing cover on unscrewing loading-unit is added the blood of 50 μ L using tools such as liquid-transfering gun or suction nozzles
Enter into agent bin 31-1.Screwing hermetic lid guarantees the leakproofness of chip；
Booting.By the power supply 12 of lower case 1, starter；
Barcode scanning typing information.By the barcode scanning region 33 on carrier element 3, the code reader 13 being aligned on shell 1, through barcode scanning
Afterwards, the corresponding detection project information of the detection system typing chip.At this point, being located at baffle 51 and the push plate 52 of liquid processing system
Initial position is reset to, there are spaces, and loading-unit 3 to be allowed to be fully inserted into from accommodating chamber 2 to equipment inside bottom；
It is inserted into loading-unit.Capping 21 is opened, loading-unit 3 is inserted into accommodating chamber 2, after closing capping 21, presses screen
The start key of curtain starts to carry out full automatic treatment analytic process；
The cracking of sample.After chip insertion, in addition to the baffle 31- (1-2) for being located at the first two position is located at initial position,
Remaining baffle moves downward, and compresses corresponding breakable seal layer 32, closes each section of agent bin 31- (2-10).Then, (baffle 51
It is moved along x-axis close to or far from loading-unit 3 with push plate 52, is described as closing baffle 51 or push plate 52 in the present embodiment
When, baffle 51 or push plate 52 are moved along x-axis close to loading-unit 3；When being described as opening baffle 51 or when push plate 52, baffle 51 or
Push plate 52 is moved along x-axis far from loading-unit 3) close the baffle 51-1 of the first position and push plate 52- of first position
1, open the baffle 51-3 of third position.Then, the push plate 52-2 for closing second position makes destroying for first position
Sealant 32 is opened under pressure, so that the sample in the 31-1 of loading storehouse be made to flow into the 31-2 of loading storehouse.Then, it alternately pushes away
Into with pull out second and third push plate 52- (2-3), mix sample and buffer back and forth in loading storehouse 31-1 and loading storehouse 31-2.
After mixing several times, second and third push plate 52- (2-3) is pulled out, and open the baffle 51-4 of the 4th position, promote the 4th push plate
52-4 destroys the breakable seal layer 32 between loading storehouse 31-3 and loading storehouse 31-2, makes the internal control liquid stream in the 31-3 of loading storehouse
Enter in the 31-2 of loading storehouse.Then alternately squeeze second and third, four push plate 52- (2,3,4) keep sample, buffer, interior draining sufficiently mixed
It is even.The position that heating region is located at loading storehouse 31-1 to loading storehouse 31-7 is heated to 56 DEG C.It similarly opens loading storehouse 31-4, carry
Object storehouse 31-5, loading storehouse 31-6, mix well sample, buffer, interior draining, protease k, magnetic-particle, lysate, are formed
Mixed liquor.Mixed liquor is squeezed to loading storehouse 31-4, loading storehouse 31-5, loading storehouse 31-6, region, the gear of the 5th position is promoted
Plate 51-5 alternately squeezes the push plate 52- (5,6,7) of the five, the six, seven positions, makes mixed liquor can only be in loading storehouse 31-4, loading storehouse
31-5, the flowing of the loading storehouse region 31-6, and cultivated 3 minutes under the conditions of 56 DEG C of temperature；
Nucleic acid is collected.Magnet is moved to the loading storehouse corresponding region 31-5, alternately squeezing push plate 52- (5,6,7) makes to mix
Liquid alternately flows in (4, the 5,6) region loading storehouse 31-, when mixed liquor passes through loading storehouse 31-6, due to the magnetic field of magnet generation
Effect, magnetic-particle gradually converge in the region over numerous cycles.At this point, open fifth gear plate 51-5, successively squeeze the (7,
6,5,4,3) waste liquid is squeezed into loading storehouse 31-1, and closes the baffle 51- of third position by (7,6,5,4,3) position push plate 52-
3, flowing downward out waste liquid not.At this point, magnetic-particle remains in loading storehouse 31-5, and nucleic acid has been incorporated into magnetic-particle
On；
Nucleic acid washing.Fifth gear plate 51-5 is closed, magnet 61 is moved to the region in addition to loading storehouse 31- (4,5,6),
To eliminate the magnetic field of area above.Then, the 8th baffle 51-8 is opened, the 8th push plate 52-8 is squeezed, it will be in the 31-7 of loading storehouse
Cleaning solution squeeze into loading Cang31-6Qu.Be then shut off ground eight baffles 51, successively squeeze (7,6,5) position push plate 52- (7,
6,5), make magnetic-particle be washed liquid to wash away back and forth.Then, magnet 61 is moved into loading Cang31-5Qu, it is described above, by magnetic
Property particle converges to loading Cang31-5Qu in scour process.Then, described above, waste liquid is transferred to loading storehouse 31-1,
And the baffle 51-3 of third position is closed, flow downward out waste liquid no longer.At this point, being located on magnetic-particle in addition to nucleic acid
Residue and mortifier etc. are removed；
Nucleic Acid Elution.Fifth gear plate 51-5 is closed, magnet 61 is maintained at the loading storehouse position 31-5.Then, the 9th is opened
Baffle 51-9 squeezes the 9th push plate 52-9, the eluent of loading Cang31-8Qu is transported to loading Cang31-5Qu.Then, it promotes
Loading Cang31-5Qu is corresponded to temperature of heating plate and is adjusted to 70 DEG C by the 8th baffle 51-8, successively repeatedly extrudes push plate 52- (7,6,5),
Eluent is set back and forth to wash away the magnetic-particle positioned at loading Cang31-5Qu from loading Cang31-4Qu to loading Cang31-6Qu, thus will
Nucleic acid is eluted from magnetic-particle；
Nucleic acid amplification and interpretation of result.The baffle 51- (8,9,10) of (8,9,10) position is successively opened, eluent is extruded into
After the 31-8 of loading storehouse, the baffle 51-9 of the 9th position is closed.Then, the baffle 51-11 of the 11st position is opened, is alternately squeezed
The push plate 52- (9,10,11) for pressing (9,10,11) position mixes eluent and PCR premixed liquid 1,2, repeats mixing movement
2min.Then, loading storehouse 31-8 is corresponded to heating region temperature to adjust to 95 DEG C, loading storehouse 31-9 corresponds to the temperature of heating region
It adjusts to 72 DEG C, loading storehouse 31-10 corresponds to heating region temperature and adjusts to 55 DEG C.Final mixed liquor is squeezed to loading storehouse 31-8
It is kept for 5 seconds, final mixed liquor is squeezed to loading storehouse 31-10 and is kept for 8 seconds, then will finally mixed also to squeeze to loading storehouse 31-9 and protect
It holds 11 seconds.It is recycled according to above 3 temperature regions and retention time as one, i.e., 95 DEG C are kept for 5 seconds, and 55 DEG C are kept for 8 seconds,
72 ° keep recycling for one for 11 seconds.And in the last 5s of each circulation, by the optical mode for corresponding to optical detection apparatus 72
Block carries out fluorescence detection.It is controlled by main control module, carries out 40 circulations in total, issue 40 exciting lights from optical module 14, from
And 40 groups of fluorescence datas are obtained, schematic diagram is shown in Figure 11,12.It will obtain data to carry out curve fitting and algorithm operation, can be wrapped
Include but be not limited to the results such as amplification curve, solubility curve, yin and yang attribute judge and concentration of specimens is quantitative.
Offal treatment.Capping 21 is opened, reacted loading-unit 3 is taken out, at according to relevant industries waste
Manage bar money handles used disposable loading-unit 3.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention.For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways.There is no necessity and possibility to exhaust all the enbodiments.It is all this
Made any modifications, equivalent replacements, and improvements etc., should be included in the claims in the present invention within the spirit and principle of invention
Protection scope within.
Claims (22)
1. a kind of nucleic acid extraction amplification system, which is characterized in that including temperature control mechanism, pushing mechanism, magnetic mechanism, loading-unit
And control unit；
The loading-unit includes carrier cavities, and the inside of the carrier cavities is equipped with multiple breakable seal layers, will be described
Carrier cavities are divided into multiple sequentially connected, mutually independent loading storehouses；The top of the carrier cavities is equipped with sealable
Sample injection port；
The side of the loading-unit is the temperature control mechanism for controlling the temperature in multiple loading storehouses respectively, and the other side is to push away
Motivation structure；The pushing mechanism includes pushing pressing mechanism and pushing to obstruct mechanism；The promotion pressing mechanism can for getting through
It destroys sealant and then is connected to adjacent loading storehouse；The fluid for pushing barrier mechanism to be used to obstruct adjacent loading storehouse is mutually gone here and there
It is dynamic；
The promotion pressing mechanism includes several baffles；Promotion barrier mechanism includes several push plates；The baffle and described
Push plate is separately connected first driving device；The baffle be closely located to the breakable seal layer can barrier fluid mutually go here and there
It is dynamic, the push plate be located proximate to the loading storehouse can be broken by fashion of extrusion the breakable seal layer realize it is adjacent described
The flowing of reagent between loading storehouse；The face being extruded in the loading storehouse be flexible structure, conducive to the push plate extruding into
Row；
The magnetic mechanism is used for the magnetic bead that is sucked in the loading storehouse and the magnetic bead can be driven to be moved to each loading storehouse
Corresponding position；
Described control unit is electrically connected in the temperature control mechanism, pushing mechanism and magnetic mechanism.
2. nucleic acid extraction amplification system according to claim 1, which is characterized in that the magnetic mechanism include magnetic part and
For driving the magnetic substance to be moved to the second driving device of each loading bin location；The magnetic part is close to the loading storehouse
Side, second driving device for realizing nucleic acid extraction and amplification during the magnetic substance movement.
3. nucleic acid extraction amplification system according to claim 2, which is characterized in that second driving device, which is equipped with, to be slided
Block, the sliding block connect the magnetic part.
4. nucleic acid extraction amplification system according to claim 3, which is characterized in that the magnetic mechanism further includes magnetic spiral shell
Rasp bar, the magnetic threaded rod screw thread are socketed the sliding block, and second driving device includes multiple motors, the motor connection
The magnetic threaded rod.
5. nucleic acid extraction amplification system according to claim 1, which is characterized in that the first driving device includes multiple
Motor, the baffle connect the motor, and the push plate connects the motor, and the motor can individually control the gear
Plate and the push plate；The quantity of the baffle, the push plate and the motor and the loading storehouse and the breakable seal layer
Quantity it is corresponding.
6. nucleic acid extraction amplification system according to claim 1, which is characterized in that the baffle includes first end and branch
Frame.
7. nucleic acid extraction amplification system according to claim 6, which is characterized in that the first end is elastic ends.
8. nucleic acid extraction amplification system according to claim 1, which is characterized in that the push plate includes the second end, pushes away
Plate heating film, temperature sensor and push plate seat；The second end is fixedly connected with push plate heating film；The push plate heating film is fixed
Connect push plate seat；The temperature sensor is fixedly connected with the push plate seat.
9. nucleic acid extraction amplification system according to claim 1, which is characterized in that the temperature conditioning unit includes heating support
Plate, heating plate, multiple heater elements and multiple temperature sensors；Fixation connects respectively for the heater element and the temperature sensor
Connect the heating plate；The heating plate is fixedly connected on the heated support plate；The heater element is located at the loading storehouse
Side.
10. nucleic acid extraction amplification system according to claim 9, which is characterized in that the heater element and the heating
Plate is fixedly connected by the way of clamping or bonding；The temperature sensor and the heating plate using clamping or it is bonding by the way of
It is fixedly connected.
11. nucleic acid extraction amplification system according to claim 9, which is characterized in that the heater element be heating film or
The combination of semiconductor or heating film and semiconductor.
12. nucleic acid extraction amplification system according to claim 9, which is characterized in that the semiconductor is TEC semiconductor.
13. a kind of molecular detector arrangement, which is characterized in that including described in shell, optical module and claim any one of 1-13
Nucleic acid extraction amplification system；
The enclosure interior is equipped with chamber resettling, and the chamber resettling is used for the nucleic acid extraction amplification system；
The optical module is used to carry out optical detection to substance in loading storehouse.
14. molecular detector arrangement according to claim 13, which is characterized in that the optical module is set to the loading storehouse
Ambient side.
15. molecular detector arrangement according to claim 14, which is characterized in that the institute of the heating region and corresponding position
Heater element is stated equipped with through-hole, the side of the through-hole is equipped with the optical module.
16. the portable molecular detector arrangement of Full enclosed integrated according to claim 13, which is characterized in that the accommodating
Chamber passes through lidstock；The capping is set on the shell.
17. the portable molecular detector arrangement of Full enclosed integrated according to claim 16, which is characterized in that the capping
The hinged or shell that is slidably connected.
18. the portable molecular detector arrangement of Full enclosed integrated according to claim 13, which is characterized in that the through-hole
Shape be round or polygon any one.
19. the portable molecular detector arrangement of Full enclosed integrated according to claim 13, which is characterized in that the through-hole
Quantity be at least 1.
20. the portable molecular detector arrangement of Full enclosed integrated according to claim 13, which is characterized in that the optics
Module includes excitation-light unit and optical detection apparatus.
21. the portable molecular detector arrangement of Full enclosed integrated according to claim 13, which is characterized in that the molecule
Detection device further includes code reader, and the code reader is set on the side wall of the shell, and the loading-unit is equipped with and institute
State the barcode scanning region that code reader matches.
22. the portable molecular detector arrangement of Full enclosed integrated according to claim 21, which is characterized in that the barcode scanning
Two dimensional code or bar code is arranged in region.
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| CN113846014A (en) * | 2021-10-29 | 2021-12-28 | 青岛全诊生物技术有限公司 | Nucleic acid extraction and amplification device |
| GB2596895A (en) * | 2020-07-09 | 2022-01-12 | Hangzhou Junling Pharmaceutical Tech Co Ltd | A nucleic acid detection kit by magnetic beads methods |
| CN114247492A (en) * | 2021-12-23 | 2022-03-29 | 淄博市产品质量检验研究院 | Rapidly-fixed detection equipment for chemical engineering inspection and use method thereof |
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