GB2596895A - A nucleic acid detection kit by magnetic beads methods - Google Patents

A nucleic acid detection kit by magnetic beads methods Download PDF

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Publication number
GB2596895A
GB2596895A GB2105151.1A GB202105151A GB2596895A GB 2596895 A GB2596895 A GB 2596895A GB 202105151 A GB202105151 A GB 202105151A GB 2596895 A GB2596895 A GB 2596895A
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support
rods
fixedly arranged
reaction
detection
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GB202105151D0 (en
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Yu Manjiang
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Hangzhou Junling Pharmaceutical Tech Co Ltd
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Hangzhou Junling Pharmaceutical Tech Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2547/00—Reactions characterised by the features used to prevent contamination
    • C12Q2547/10—Reactions characterised by the features used to prevent contamination the purpose being preventing contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

Abstract

A nucleic acid detection kit comprising a reaction mechanism, and extraction mechanism, and a detection mechanism. The extraction mechanism comprises an extraction plate 14 with six magnetic strip spaces 15 detachably and uniformly arranged on the extraction plate. Five stop blocks (limiting blocks) 18 are fixedly arranged on a lower end of the extraction plate uniformly arranged between the magnetic strip spaces. Stopping slide slots 19 are detachably arranged on the first stop blocks. First compression springs 20 are fixedly arranged on lower ends of the stopping slide slots. Support assemblies are arranged o the lower end of the compression springs. The reaction mechanism comprises an outer shell 10, having a plurality of reaction boxes are detachably arranged in the outer shell. The reaction boxes comprise reaction chambers 11 with reaction spaces 38 within. Two high baffles 12 and two low baffles 13 are fixedly arranged on the upper ends of the reaction chambers. The detection mechanism comprises a framework 28 with detection chambers 29 detachably arranged thereto. A detection space is arranged in each detection chamber. A blocking assembly is detachably arranged on the detection chamber.

Description

A NUCLEIC ACID DETECTION KIT BY MAGNETIC BEADS METHODS
TECHNICAL FIELD
[0001] The present invention relates to the field of nucleic acid detection, in particular to a nucleic acid detection kit by magnetic beads methods.
BACKGROUND OF THE INVENTION
[0002] A nucleic acid detection kit by magnetic beads methods plays a important role in nucleic acid detection, which is one of the necessary tools in nucleic acid extraction and detection, and traditional nucleic acid detection kit is simple in structure, which have no effective separation and protection device for magnetic beads with extracted nucleic acid, thereby the magnetic beads are easily to be collided under an action of a magnetic force, thereby a cross-infection is caused, and when the magnetic beads are placed into detection boxes after an extraction, the magnetic beads are hard to be separated under an action of magnetic rod.
BRIEF SUMMARY OF THE INVENTION
[0003] The technical problem to be solved by the invention is to provide a nucleic acid detection kit by magnetic beads methods which is convenient to so as to overcome the problems existing in the prior art.
[0004] The following technical plan is adopted by the invention to solve the above-mentioned technical problems: a nucleic acid detection kit by magnetic beads methods comprises a reaction mechanism, an extraction mechanism and a detection mechanism, wherein the extraction mechanism comprises an extraction plate, wherein six of magnetic strip spaces are detachably arranged on the extraction plate, wherein five of first stop blocks are fixedly arranged on a lower end of the extraction plate, wherein stopping slide slots are detachably arranged on the first stop blocks, wherein first compression springs are fixedly arranged on lower ends of the stopping slide slots, wherein support assemblies are fixedly arranged on lower ends of the first compression springs, and six of the magnetic strip spaces are uniformly arranged on the extraction plate, and five of the first stop blocks are uniformly arranged between each two of the magnetic strip spaces.
[0005] The supporting assemblies comprise support boxes fixedly arranged on lower ends of the first compression springs, wherein two of support rods are rotatably arranged on the support boxes, wherein torsion springs are fixedly arranged within the support rods, and sliding slots are fixedly arranged on lower ends of the support boxes, wherein sliding rods are reciprocally and slidably arranged on the sliding slots, wherein guide plates are fixedly arranged on upper ends of the sliding rods, wherein threads are fixedly arranged between the guide plates and the support rods, and support plates are fixedly arranged on lower ends of the sliding rods, and each two of the support rods are bilaterally symmetric about corresponding one of the support boxes, wherein one ends of the torsion springs are fixedly connected to the support rods and another ends of the torsion springs are fixedly connected to the support boxes, and the sliding rods penetrate through the sliding slots, and one ends of the sliding rods are placed within the support boxes and another ends of the sliding rods are placed within the sliding slots, wherein the support plates are able to reciprocally slide within the sliding slots [0006] The supporting assemblies further comprise two of the second compression springs fixedly arranged between upper end surfaces of the support plates and upper inner walls of the sliding slots, wherein two of the second compression springs are bilaterally symmetric about the sliding rods.
[0007] The reaction mechanism comprises an outer shell, wherein a plurality of reaction boxes are detachably arranged in the outer shell, wherein the reaction boxes comprise the reaction chambers, wherein the reaction spaces are arranged within the reaction chambers, wherein two of the high baffles and two of the low baffles are fixedly arranged on upper ends of the reaction chambers, and two of the high baffles and two of the low baffles are fixedly connected in sequence.
[0008] The detection mechanism comprises the framework, wherein detection chambers are detachably arranged in the framework, wherein a detection space is arranged in the detection chambers, wherein a blocking assembly is detachably arranged on the detection chambers [0009] The blocking assemblies comprise U-shaped grooves, wherein connect rods are rotatably arranged on the U-shaped grooves, wherein third compression springs are fixedly arranged on lower end surfaces of the connect rods, wherein arc-shaped plates are fixedly arranged on another ends of the third compression springs, wherein spherical blocks are rotatably arranged in the arc-shaped plates, wherein second stop blocks are fixedly arranged on the spherical blocks, wherein the arc-shaped plates encircle two third of the spherical blocks.
[0010] Furthermore, the present invention also provides a using method of the nucleic acid detection kit by magnetic beads methods, comprising following steps: before a nucleic acid extraction is performed, in terms of a detection number, reaction boxes with corresponding number are placed in the outer shell, thereby a waste of a kit due to a using of the entire kit is avoided; when in placing, the high baffles are placed along a number direction while the low baffles are placed along a letter direction, thereby a following extraction of the magnetic beads is convenient to be done; blood preparations of testers are placed into the reaction spaces of the reaction chambers, and then the magnetic beads are placed into the reaction spaces, and then the nuclear acid is waited to be absorbed by the magnetic beads; after the extraction is performed, in terms of a nucleic acid detection number, the magnetic strip spaces with corresponding number are chosen to be placed on the extraction plate, and at the same time, the supporting assemblies are placed on the lower end of the extraction plate, and when in a placement, the stopping slide slots are driven to slide into the first stop blocks along tracks of the first stop blocks, and then the supporting assemblies are fixed on the lower end of the extraction plate, thereby the supporting assemblies are located between two of the magnetic strip spaces; in an initial state, under actions of the torsion springs, two of the support rods are in a level condition which forms stiff supports to the magnetic strip spaces on two sides of the supporting assemblies, thereby the extraction mechanism is more stable; when the magnetic beads are extracted, the magnetic strips are placed into the magnetic strip spaces, and then the extraction mechanism is placed into the reaction spaces of the reaction chambers, thereby openings of the sliding slots are placed above the low baffles; when the extraction mechanism is descending, lower end surfaces of the support plates contact upper end surfaces of the low baffles, with an increasingly descending depth, the support plates move upwards within the sliding slots through the sliding rods, thereby the second compression springs are compressed to produce the elastic energy, and then the sliding rods move upwards to drive the guide plates to move upwards, thereby a pulling force produced by the guide plates through the threads is acted on the support rods, thereby the support rods have a downward angle shift, and then the torsion springs accumulate powers, thereby the stiff supports on two of the support rods to the magnetic strip spaces translate into soft supports, thereby whole of the extraction mechanism moves more smoothly in the reaction spaces, thereby a scratch to inner walls of the framework when the extraction mechanism moves downwards is avoided, and at the same time, whole of the support assemblies move upwards between two of the magnetic strip spaces, thereby the first compression springs are compressed; when the magnetic bar contacts the magnetic beads, the magnetic beads are attracted by the magnetic bar, and then the extraction mechanism moves upwards, thereby the magnetic beads are taken out from the reaction spaces; when the extraction mechanism moves upwards, the first compression springs reset to drive the support assemblies to descend to the initial position, and at the same time, two of the second compression springs are reset to push the support plates to move downwards, and then the support plates drive the guide plates to move downwards through the sliding rods in descending, thereby the threads reset, and at the same time, the torsion springs which are accumulating energy are reset, thereby two of the support rods are rotated to the initial position, thereby the stiff supports are formed again to the magnetic strip spaces on two sides of the support assemblies, thereby a mutual attraction of two of the magnetic strip spaces under a magnetic force of the magnetic bar is avoided, thereby the magnetic beads do not contact with each other, thereby a cross infection does not caused; at the same time, the sliding slots of the supporting assemblies in descending extend into lower ends of the magnetic strip spaces, thereby a baffler is formed by the magnetic beads attracted under the magnetic strip spaces, thereby the magnetic beads are located within separate spaces, thereby an occasion that the magnetic beads contact with each other is avoided again; after an extraction is performed, the magnetic beads are placed before the detection mechanism, similarly, the detection chambers with corresponding number are placed into the framework in terms of a different detection number, thereby the waste of the detection boxes is avoided; two of the U-shaped grooves are placed on upper ends of the detection chambers, thereby two of the second stop blocks on two of the blocking assemblies are respectively contact two side inner walls of the detection space, and when the magnetic beads are placed, the magnetic beads contact upper end surfaces of the connect rods at first, and then with a downward movement of the magnetic beads, the connect rods are compressed on the U-shaped grooves to have a downward angle shift, and then the connect rods rotate to compress the third compression springs to produce an elastic energy, and then the third compression springs act on the arc-shaped plates to have an angle shift on the spherical blocks to exert a pressures on the second stop blocks, thereby a friction force between the second stop blocks and the detection space is enlarged, and with an increasing downward movements of the magnetic beads, the connect rods rotate in the increasing angle, and after the magnetic beads completely enter into the detection space, the magnetic strip spaces move upwards, arid as a limit angle formed by the connect rods obstruct the magnetic beads, thereby the magnetic strip spaces is more easily separated out of the detection space, and the magnetic beads fall into the detection space, thereby a bring-out of the magnetic beads when the magnetic strip spaces move upwards is avoided, thereby unnecessary troubles do not caused; after the magnetic strip spaces are removed, the third compression springs reset to push the connect rods to rotate in an upward angle on the U-shaped grooves, thereby two of the connect rods hole the line, thereby over contacting of the magnetic beads with an external space is avoided, thereby a pollution does not caused.
[0011] The benefits of the invention are as follows: the present invention is simple in structure, the support assemblies and the blocking assemblies are detachably arranged, thereby a using number is chosen in terms of a detection number, thereby a waste is avoided; moreover, the support assemblies act on the extraction mechanism in the detection, thereby the mutual contact of the magnetic beads due to the magnetic force is avoided, thereby the cross-infection is avoided; when the blocking assemblies enter into the detection boxes, the magnetic beads are controlled to be more easily to be separated, thereby the bring-out of the magnetic beads from polluted reagent due to the magnetic force is avoided.
BRIEF DESCRIPTION OF THE DRAWINGS
[0012] FIG. 1 s a diagram of an embodiment of a reaction mechanism in the present invention; [0013] FIG. 2 is a schematic structural diagram of "reaction boxes in FIG. 1; [0014] FIG. 3 is a diagram of an embodiment of extraction mechanism in the present invention, [0015] FIG. 4 is an enlarged diagram of "A" in FIG. 3; [0016] FIG. 5 is an enlarged diagram of "B" in FIG. 3; [0017] FIG. 6 is a diagram of an embodiment of a detection mechanism in the present invention; [0018] FIG. 7 is a schematic structural diagram of detection boxes in FIG. 6; [0019] FIG. 8 is an enlarged diagram of "C" in FIG. 7; [0020] FIG. 9 is an enlarged diagram of -D" in FIG. 7;
DETAILED DESCRIPTION OF THE INVENTION
[0021] Referring to figures 1-9, a nucleic acid detection kit by magnetic beads methods comprises a reaction mechanism, an extraction mechanism and a detection mechanism, wherein the extraction mechanism comprises an extraction plate 14, wherein six of magnetic strip spaces 15 are detachably arranged on the extraction plate 14, wherein five of first stop blocks 18 are fixedly arranged on a lower end of the extraction plate 14, wherein stopping slide slots [9 are detachably arranged on the first stop blocks 18, wherein first compression swings 20 are fixedly arranged on lower ends of the stopping slide slots 19, wherein support assemblies are fixedly arranged on lower ends of the first compression springs 20. and six of the magnetic strip spaces 15 are uniformly arranged on the extraction plate 14, and five of the first stop blocks 18 are uniformly arranged between each two of the magnetic strip spaces 15; whereby in terms of a nucleic acid detection number, the magnetic strip spaces 15 with corresponding number are chosen to be placed on the extraction plate 14, and at the same ti the supporting assemblies are mounted on the lower end of the extraction plate 14, and when in an arrangement, the stopping slide slots 19 are driven to slide into the first stop blocks 18 along tracks of the first stop blocks 18, and then the supporting assemblies are fixed on the lower end of the extraction plate 14, thereby the supporting assemblies are located between two of the magnetic strip spaces 15.
[0022] The supporting assemblies comprise support boxes 22 fixedly arranged oil lower ends of the first compression springs 20, two of support rods 16, torsion springs 21, sliding slots 17, sliding rods 25, guide plates 24, threads 23 and support plates 26, wherein two of the support rods 16 are rotatably arranged on the support boxes 22, wherein the torsion springs 21 are fixedly arranged within the support rods 16, and the sliding slots 17 are fixedly arranged on lower ends of the support boxes 22, wherein the sliding rods 25 are reciprocally and slidably arranged on the sliding slots 17, wherein the guide plates 24 are fixedly arranged on upper ends of the sliding rods 25, wherein the threads 23 are fixedly arranged between the guide plates 24 and the support rods 16, and the support plates 26 are fixedly arranged on lower ends of the sliding rods 25, and each two of the support rods 16 are bilaterally symmetric about corresponding one of the support boxes 22, wherein one ends of the torsion springs 21 are fixedly connected to the support rods 16 and another ends of the torsion springs 21 are fixedly connected to the support boxes 22, and the sliding rods 25 penetrate through the sliding slots 17, and one ends of the sliding rods 25 are placed within the support boxes 22 and another ends of the sliding rods 25 are placed within the sliding slots 17, wherein the support plates 26 are able to reciprocally slide within the sliding slots 17; whereby in an initial state, under actions of the torsion springs 21, two of the support rods 16 are in a level condition which forms a stiff support to the magnetic strip spaces 15 on two sides of the supporting assemblies, thereby the extraction mechanism is more stable; when the magnetic beads 31 are extracted, magnetic strips are placed into the magnetic strip spaces 15, and then the extraction mechanism is placed into reaction spaces 38 of reaction chambers 11, thereby openings of the sliding slots 17 are placed above low baffles 13; when the extraction mechanism is descending, lower end surfaces of the support plates 26 contact upper end surfaces of the low baffles 13, with an increasingly descending depth, the support plates 26 move upwards within the sliding slots 17 through the sliding rods 25" thereby second compression springs 27 are compressed to produce an elastic energy, and then the sliding rods 25 move upwards to drive the guide plates 24 to move upwards, thereby a pulling force produced by the guide plates 24 through the threads 23 is acted on the support rods 16, thereby the support rods 16 have an downward angle shift, and then the torsion springs 21 accumulate powers, thereby the stiff supports of two of the support rods 16 to the magnetic strip spaces 15 translate into soft supports, thereby whole of the extraction mechanism moves more smoothly in the reaction spaces 38, thereby a scratch to inner wails of a framework 28 when the extraction mechanism moves downwards is avoided, and at the same time, whole of the support assemblies move upwards between two of the magnetic strip spaces 15, thereby the first compression springs 20 are compressed; when the magnetic bar contacts the magnetic heads 31, the magnetic beads 31 are attracted by the magnetic bar, and then the extraction mechanism moves upwards, thereby the magnetic beads 31 are taken out from the reaction spaces 38; when the extraction mechanism moves upwards, the first compression springs 20 reset to drive the support assemblies to descend to an initial position, and at the same time, two of the second compression springs 27 are reset to push the support plates 26 to move downwards, and then the support plates 26 drive the guide plates 24 to move downwards through the sliding rods 25 in descending, thereby the threads 23 reset, and at the same time, the torsion springs 2 which are accumulating energy are reset, thereby two of the support rods 16 are rotated to the initial position, thereby the stiff supports are formed again to the magnetic strip spaces 15 on two sides of the support assemblies, thereby a mutual attraction of two of the magnetic strip spaces 15 under a magnetic force of the magnetic bar is avoided, thereby the magnetic beads 31 do not contact with each other, thereby a cross infection does not caused; at the same time, the sliding slots 17 of the supporting assemblies in descending extend into lower ends of the magnetic strip spaces 15, thereby a baffler is formed by the magnetic beads 31 attracted under the magnetic strip spaces 15, thereby the magnetic beads 31 are located within separate spaces, thereby an occasion that the magnetic beads 31 contact with each other is avoided again.
[0023] The supporting. assemblies further comprise two of the second compression springs 27 fixedly arranged between upper end surfaces of the support plates 26 a.nd upper inner walls of the sliding slots 17, wherein two of the second compression springs 27 are bilaterally symmetric about the sbding rods 25.
[0024] The reaction mechanism comprises an outer shell 10, Wherein a plurality of reaction boxes are detachably arranged in the outer shell 10, wherein the reaction boxes comprise the reaction chambers Ii, the reaction spaces 38, two of high baffles 12 and two of the low baffles 13 wherein the reaction spaces 38 are arranged within the reaction chambers 11, wherein two of the high baffles 12 and two of the low baffles 13 are fixedly arranged on upper ends of the reaction chambers 11, and two of the high baffles 12 and two of the low baffles 13 are fixedly connected in sequence; whereby before a nucleic acid extraction is performed, in terms of the detection number, the reaction boxes \vith corresponding number are placed in the outer shell 10, thereby a waste of kit due to a using of entire kit is avoided; when in placing, the high baffles 1_2 are placed along a number direction while the low baffles 13 are placed along a letter direction, thereby a following extraction of the magnetic beads 31 is convenient to be done, and blood preparations of testers are placed into the reaction. spaces 38 of the reaction chambers 11, and then the magnetic beads 31 are placed into the reaction spaces 38, and then the nuclear acid is waited to be absorbed by the magnetic beads 31.
[0025] The detection mechanism comprises the framework 28, detection chambers 29, a detection space 37 and a blocking assembly, wherein the detection chambers 29 are detachably arranged in the framework 28, wherein the detection space 37 is arranged in the detection chambers 29, wherein the blocking assembly is detachably arranged on the detection chambers 29; Whereby after the extraction is performed, the magnetic beads 31 are placed before the detection mechanism, similarly, the detection chambers 29 with corresponding number are placed into the framework 28 in terms of different detection number, thereby a waste of the detection boxes is avoided.
[0026] The blocking assemblies comprise U-shaped grooves 30, connect rods 33, third compression springs 32, arc-shaped plates 34, spherical blocks 35 and second stop blocks 36, wherein the connect rods 33 are rota.tably arranged on the U-shaped grooves 30, wherein the third compression springs 32 are fixedly arranged on lower end surfaces of the connect rods 33, wherein the arc-shaped plates 34 are fixedly arranged on another ends of the third compression springs 32, wherein the spherical blocks 35 are rotatably arranged in the arc-shaped plates 34, wherein the second stop blocks 36 are fixedly arranged on the spherical blocks 35, wherein the arc-shaped plates 34 encircle two third of the spherical blocks 35; whereby two of the U-shaped grooves 30 are placed on upper ends of the detection chambers 29, thereby two of the second stop blocks 36011 two of the blocking assemblies are respectively contact two side inner walls of the detection space 37, and when the magnetic beads 31 are placed, the magnetic beads 31 contact upper end surfaces of the connect rods 33 at first, and then with a downward movement of the magnetic beads 31, the connect rods 33 are compressed on the U-shaped grooves 30 to have a downward angle shift, and then the connect rods 33 rotate to compress the third compression springs 32 to produce the elastic energy, and then the third compression springs 32 act on the arc-shaped plates 34 to have an angle shift on the spherical blocks 35 to exert pressures on the second stop blocks 36, thereby a friction force between the second stop blocks 36 and the detection space 37 is enlarged, and with an increasing downward movement of the magnetic beads 31, the connect rods 33 rotate in an increasing angle, and after the magnetic beads 31 completely enter into the detection space 37, the magnetic strip spaces 15 move upwards, and as a limit angle formed by the connect rods 33 obstruct the magnetic beads 31, thereby the magnetic strip spaces 15 are more easily separated out of the detection space 37, and then the magnetic beads 31 fall into the detection space 37, thereby a bring-out of the magnetic beads 31 when the magnetic strip spaces 15 move upwards is avoided, thereby unnecessary troubles do not caused; after the magnetic strip spaces 15 is removed, the third compression springs 32 reset to push the connect rods 33 to rotate in an upward angle on the U-shaped grooves 30, thereby two of the connect rods 33 hole the line, thereby over contacting of the magnetic beads 31 with an external space is avoided, thereby a pollution does not caused.
[0027] Working principle: before the nucleic acid extraction is performed, in terms of the detection number, the reaction boxes with corresponding number are placed in the outer shell 10, thereby the waste of the kit due to the using, of the entire kit is avoided; when in placing, the high baffles 12 are placed along a number direction while the low baffles 13 are placed along a. letter direction, thereby the following extraction of the magnetic beads 31 is convenient to be done; the blood preparations of the testers are placed into the reaction spaces 38 of the reaction chambers II, and then the magnetic beads 31 are placed into the reaction spaces 38, and then the nuclear acid is waited to be absorbed by the magnetic beads 31; after the extraction is performed, in terms of the nucleic acid detection number; the magnetic strip spaces 15 with corresponding number are chosen to be placed on the extraction plate 14, and at the same time, the supporting assemblies are placed on the lower end of the extraction plate 14, and when in the placement, the stopping slide slots 19 are driven to slide into the first stop blocks 18 along the tracks of the first stop blocks 18, and then the supporting assemblies are fixed on the lower end of the extraction plate 14, thereby the supporting assemblies are located between two of the magnetic strip spaces 15; in the initial state, under the actions of the torsion springs 21, two of the support rods 16 are in the level condition which forms the stiff supports to the magnetic strip spaces 15 on two sides of the supporting assemblies, thereby the extraction mechanism is more stable; when the magnetic beads 31 are extracted; the magnetic strips are placed into the magnetic strip spaces 15, and then the extraction mechanism is placed into the reaction spaces 38 of the reaction chambers 11, thereby the openings of the sliding slots 17 are placed above the low baffles 13; when the extraction mechanism is descending, the lower end surfaces of the support plates 26 contact the upper end surfaces of the low baffles 13, with the increasingly descending depth, the support plates 26 move upwards within the sliding slots 17 through the sliding rods 25, thereby the second compression springs 27 are compressed to produce the elastic energy, and then the sliding rods 25 move upwards to drive the guide plates 24 to move upwards, thereby the pulling force produced by the guide plates 24 through the threads 23 is acted on the support rods 16, thereby the support rods 16 have the downward angle shift, and then the torsion springs 21 accumulate powers, thereby the stiff supports on two of the support rods 16 to the magnetic strip spaces 15 translate into the soft supports, thereby whole of the extraction mechanism moves more smoothly in the reaction spaces 38, thereby the scratch to the inner walls of the framework 28 when the extraction mechanism moves downwards is avoided, and at the same time, whole of the support assemblies move upwards between two of the magnetic strip spaces 15, thereby the first compression springs 20 are compressed; when the magnetic bar contacts the magnetic beads 31, the magnetic beads 31 are attracted by the magnetic bar, and then the extraction mechanism moves upwards, thereby the magnetic heads 31 are taken out from the reaction spaces 38; When the extraction mechanism moves upwards, the first compression springs 20 reset to drive the support assemblies to descend to the initial position, and at the same time, two of the second compression springs 27 are reset to push the support plates 26 to move downwards, and then the support plates 26 drive the guide plates 24 to move downwards through the sliding rods 25 in descending, thereby the threads 23 reset, and at the same time, the torsion springs 21 which are accumulating energy are reset, thereby two of the support rods 16 are rotated to the initial position, thereby the stiff supports are formed again to the magnetic strip spaces 15 on two sides of the support assemblies, thereby the mutual attraction of two of the magnetic strip spaces 15 under the magnetic force of the magnetic bar is avoided, thereby the magnetic beads 31 do not contact with each other, thereby the cross infection does not caused; at the same time, the sliding slots 17 of the supporting assemblies in descending extend into lower ends of the magnetic strip spaces 15 thereby the baffler is formed by the magnetic beads 31 attracted under the magnetic strip spaces 15, thereby the magnetic beads 31 are located within separate spaces, thereby the occasion that the magnetic beads 31 contact with each other is avoided again; after the extraction is performed, the magnetic beads 31 are placed before the detection mechanism, similarly, the detection chambers 29 with corresponding number are placed into the framework 28 in terms of the different detection number, thereby the waste of the detection boxes is avoided: two of the U-shaped grooves 30 are placed on the upper ends of the detection chambers 29, thereby two of the second stop blocks 36 on two of the blocking assemblies are respectively contact two side inner walls of the detection space 37, and when the magnetic beads 31 are placed, the magnetic beads 31 contact the upper end surfaces of the connect rods 33 at first,and then with the downward movcment of the magnetic beads 31, the connect rods 33 are compressed on the U-shaped grooves 30 to have the downward angle shift, and then the connect rods 33 rotate to compress the third compression springs 32 to produce the elastic energy, and then the third compression springs 32 act on the arc-shaped plates 34 to have the angle shift on the spherical blocks 35 to exeit the pressures on the second stop blocks 36, thereby the friction force between the second stop blocks 36 and the detection space 37 is enlarged, and with the increasing downward movements of the magnetic beads 31, the connect rods 33 rotate in the increasing angle, and after the magnetic beads 31 completely enter into the detection space 37, the magnetic strip spaces H move upwards, and as the limit angle formed by the connect rods 33 obstruct the magnetic beads 31; thereby the magnetic strip spaces 15 is more easily separated out of the detection space 37, and the magnetic beads 31 fall into the detection space 37, thereby the bring-out of the magnetic beads 31 when the magnetic strip spaces 15 move upwards is avoided, thereby the unnecessaty troubles do not caused; after the magnetic strip spaces 15 are removed, the third compression springs 32 reset to push the connect rods 33 to rotate in the upward angle on the 1U-shaped grooves 30, thereby two of tile connect rods 33 hole the line, thereby over contacting of the magnetic beads 31 with external space is avoided, thereby the pollution does not caused.
[0028] The above shows and illustrates the basic principles, main features, and advantages of the present invention. Persons skilled in the art should understand that, the invention is not subject to restrictions of the embodiments above, the embodiments and descriptions stated above only describe the principle of the present invention. The invention will be subject to modification and improvement based on its intention and extent, which will also fall into the claimed protection extent of this invention. The claimed protection extent of the invention shall be determined with reference to the appended claims and any equivalents thereof.

Claims (4)

  1. CLAIMSI. A nucleic acid detection kit by magnetic beads methods comprising: a reaction mechanism; an extraction mechanism and a detection mechanism, wherein the extraction mechanism comprises an extraction plate, wherein six of magnetic strip spaces are detachably arranged on the extraction plate, wherein five of first stop blocks are fixedly arranged on a lower end of the extraction plate, wherein stopping slide slots are detachably arranged on the first stop blocks, wherein first compression springs are fixedly arranged on lower ends of the stopping slide slots, wherein support assemblies are fixedly arranged on lower ends of the first compression springs, six of the magnetic strip spaces uniformly arranged on the extraction plate; five of the first stop blocks uniformly arranged between each two of the magnetic strip spaces; the reaction mechanism comprising: an outer shell, wherein a plurality of reaction boxes are detachably arranged in the outer shell, wherein the reaction boxes comprise the reaction chambers, wherein the reaction spaces are arranged within the reaction chambers, wherein two of the high baffles and two of the low baffles are fixedly arranged on upper ends of the reaction chambers; two of the high baffles and two of the low baffles fixedly connected in sequence, the detection mechanism comprising: the framework, wherein detection chambers are detachably arranged in the framework, wherein a detection space is arranged in the detection chambers, wherein a blocking assembly is detachably arranged on the detection chambers.
  2. 2. The nucleic acid detection kit by magnetic beads methods defined in claim 1, wherein the supporting assemblies comprising: support boxes fixedly arranged on lower ends of the first compression springs, wherein two of support rods are rotatably arranged on the support boxes, wherein torsion springs are fixedly arranged within the support rods; sliding slots fixedly arranged on lower ends of the support boxes, wherein sliding rods are reciprocally and slidably arranged on the sliding slots, wherein guide plates are fixedly arranged on upper ends of the sliding rods, wherein threads are fixedly arranged between the guide plates and the support rods, support plates fixedly arranged on lower ends of the sliding rods; each two of the support rods bilaterally symmetric about corresponding one of the support boxes, wherein one ends of the torsion springs are fixedly connected to the support rods and another ends of the torsion springs are fixedly connected to the support boxes, and the sliding rods penetrate through the sliding slots, and one ends of the sliding rods are placed within the support boxes and another ends of the sliding rods are placed within the sliding slots, wherein the support plates are able to reciprocally slide within the sliding slots.
  3. 3. The nucleic acid detection kit by magnetic beads methods defined in claim 2, wherein the supporting assemblies further comprise two of the second compression springs fixedly arranged between upper end surfaces of the support plates and upper inner walls of the sliding slots, wherein two of the second compression springs are bilaterally symmetric about the sliding rods.
  4. 4. The nucleic acid detection kit by magnetic beads methods defined in claim 1, wherein the blocking assemblies comprising: U-shaped grooves, wherein connect rods are rotatably arranged on the U-shaped grooves, wherein third compression springs are fixedly arranged on lower end surfaces of the connect rods, wherein arc-shaped plates are fixedly arranged on another ends of the third compression springs, wherein spherical blocks are rotatably arranged in the arc-shaped plates, wherein second stop blocks are fixedly arranged on the spherical blocks, wherein the arc-shaped plates encircle two third of the spherical blocks.
GB2105151.1A 2020-07-09 2021-04-12 A nucleic acid detection kit by magnetic beads methods Pending GB2596895A (en)

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CN202010654730.9A CN111647503B (en) 2020-07-09 2020-07-09 Magnetic bead method nucleic acid detection kit

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