CN108795701A

CN108795701A - Nucleic acid extraction mechanism, sample processing device and method for extracting nucleic acid

Info

Publication number: CN108795701A
Application number: CN201810846476.5A
Authority: CN
Inventors: 曹玉峰
Original assignee: Shandong See Microbiology Technology Co Ltd
Current assignee: Shandong See Microbiology Technology Co Ltd
Priority date: 2018-07-27
Filing date: 2018-07-27
Publication date: 2018-11-13

Abstract

This disclosure relates to a kind of nucleic acid extraction mechanism, sample processing device and method for extracting nucleic acid, nucleic acid extraction mechanism is arranged in the microscope carrier transport establishment and using the sample container extraction nucleic acid with multiple sample holding tanks, including：Pipette tips mobile device, can up and down it be arranged in microscope carrier transport establishment along short transverse, the sampling hammerhead in pipette tips mobile device is separatably set along short transverse shift position for driving, and enables to the liquid in sampling hammerhead absorption sample container or the liquid in discharge sampling hammerhead；Magnetic bead transfer device, including magnetic part, magnetic part can be telescopically arranged along the direction closer or far from sampling hammerhead in microscope carrier transport establishment, so that magnetic part has the demagnetizing state for the magnetic force for acting on the magnetic bead in sampling hammerhead for magnetic state and removing for providing the magnetic bead in sampling hammerhead magnetic force.Nucleic acid extraction cost can be reduced by the nucleic acid extraction mechanism of structure as described above and improves nucleic acid extraction operating efficiency.

Description

Nucleic acid extraction mechanism, sample processing device and method for extracting nucleic acid

Technical field

This disclosure relates to sample process technical field, and in particular, to a kind of nucleic acid extraction mechanism, sample processing device with And method for extracting nucleic acid.

Background technology

In existing sample processing device, the movable working arm of generally use is each on fixed microscope carrier to being loaded into Sample container executes the processing operations such as dispensing sample, extraction nucleic acid, detection nucleic acid successively.For example, for nucleic acid extraction operation Generally by paramagnetic particle method and using microscope carrier and sample container relative to microscope carrier transport establishment it is fixed in the way of.In the case, During nucleic acid extraction, in the state that bead complexes are placed in sample container always, drawn by sampling hammerhead Waste liquid in sample container and the mode for injecting new reaction reagent execute nucleic acid extraction operation.Specifically, in core in existing After a certain reaction reagent in acid-bead complexes and sample container is sufficiently mixed, magnetic force generation device is to sample container There is provided magnetic force so that nucleic acid-bead complexes are adsorbed on sample container inner wall.At this point, mechanical arm driving sampling hammerhead is inhaled It samples the reaction reagent waste liquid in this container and excludes, while the sampling hammerhead that discarded use is crossed, mechanical arm fills again later New sampling hammerhead is carried another reaction reagent is injected into sample container and is mixed with nucleic acid-bead complexes It closes, to execute nucleic acid extraction operation successively.Therefore, this nucleic acid extraction mode uses one since a reaction reagent corresponds to A sampling hammerhead leads to the serious waste that sampling hammerhead occurs, increases nucleic acid extraction cost, and reduce nucleic acid extraction operation Efficiency.

Invention content

Purpose of this disclosure is to provide a kind of cores that can reduce nucleic acid extraction cost and improve nucleic acid extraction operating efficiency The nucleic acid extraction of the sample processing device and the utilization nucleic acid extraction mechanism of sour extraction mechanism including the nucleic acid extraction mechanism Method.

To achieve the goals above, according to one aspect of the disclosure, a kind of nucleic acid extraction mechanism is provided, using with more The sample container of a sample holding tank extracts nucleic acid, and the nucleic acid extraction mechanism is arranged in the microscope carrier transport establishment, and Including：Pipette tips mobile device, the pipette tips mobile device can be up and down arranged in the microscope carrier transport establishment along short transverse, The sampling hammerhead in the pipette tips mobile device is separatably set along short transverse shift position for driving, and can So that sampling hammerhead adsorbs the liquid in the sample container or the liquid in the discharge sampling hammerhead；Magnetic bead transfer dress It sets, which includes magnetic part, and the magnetic part can be flexible along the direction closer or far from the sampling hammerhead Ground is arranged in the microscope carrier transport establishment, so that the magnetic part has the confession for providing the magnetic bead in sampling hammerhead magnetic force Magnetic state and removing act on the demagnetizing state of the magnetic force of the magnetic bead in sampling hammerhead.

Optionally, the pipette tips mobile device includes the pedestal being arranged in the microscope carrier transport establishment and can be along described The ejection releasing mechanism of the pipette tips on the pedestal is arranged in short transverse up and down, and it includes for pacifying which, which ejects releasing mechanism, The sampling gun, sampling gun actuator and ejection release piece, the driving portion of the sampling gun actuator for filling sampling hammerhead take with described The telescopic rod of sample rifle connects and is used to drive the telescopic rod flexible, so that the sampling gun provides suction for the sampling hammerhead The suction of liquid or the pressure of drain, the ejection release piece are mounted on the sampling gun actuator and enable to the sampling Pipette tips are detached with the sampling gun.

Optionally, the sampling gun includes main body and multiple telescopic rods, and the main body setting is driven in the sampling gun It is each described to stretch on the fixed pedestal of moving part, and with the multiple telescopic rod channels for the both ends open being inserted into for the telescopic rod The top of contracting bar is connect with the driving portion of the sampling gun actuator, and the driving that each telescopic rod passes through the driving portion And can be telescopically inserted into along short transverse in corresponding telescopic rod channel, the main body corresponds to each institute State telescopic rod channel bottom be respectively structured as with the pipette tips interconnecting piece of corresponding sampling hammerhead connection, the ejection Release piece is configured to move relative to each pipette tips interconnecting piece, with enable to each sampling hammerhead with respectively The corresponding pipette tips coupling part from.

Optionally, the ejection release piece includes that can be supported on the sampling gun driving up and down along the short transverse Ejection disengaging plate on the fixed pedestal of part, the ejection disengaging plate are located at the lower section of the main body in the short transverse, and It is formed with multiple pipette tips interconnecting pieces on the ejection disengaging plate and is inserted into hole, the pipette tips linkage section of each pipette tips interconnecting piece is respectively The pipette tips interconnecting piece is accordingly penetrated through to be inserted into hole and be exposed to the side of the ejection disengaging plate, the sampling gun actuator Driving portion is set as, and under the retracted mode that the telescopic rod is retracted, the ejection disengaging plate can be driven along sampling hammerhead Off-direction squeezes the sampling hammerhead on each pipette tips interconnecting piece.

Optionally, the ejection release piece includes supporting plate and supporting plate and the ejection disengaging plate is connect with described Connecting rod, the plate of supporting are located between the driving portion and the main body of the sampling gun actuator, the plate and described supported Ejection disengaging plate can be up and down arranged along the short transverse on the fixed pedestal by the connecting rod, the driving Portion can abut under the retracted mode with the plate of supporting, to push the ejection release piece to be moved along the off-direction Dynamic, the connecting rod is resiliently supported at by the resetting spring being set in the connecting rod on the fixed pedestal, the reset Plate is supported and the fixed pedestal abuts in the both ends of spring with described respectively, and the ejection release piece is driven always for providing Return back to the elastic-restoring force of initial position.

Optionally, the sampling gun has been positioned apart from multiple in transverse direction, described to support plate and ejection disengaging Plate extends along the horizontal direction respectively, and the connecting rod is multiple and is taken respectively there are one described along the horizontal direction interval The mode of sample rifle, which is arranged, to be supported described between plate and the ejection disengaging plate, so that described support plate, ejection disengaging Sampling gun layout area, the main body position of each sampling gun are respectively constituted between connecting rod described in plate and adjacent each two In in the sampling gun layout area.

Optionally, the sampling gun actuator includes the fixed pedestal, the sampling gun that is arranged on the fixed pedestal drives Dynamic motor, the sampling gun driver for being connected and being provided with the driving portion with the transmission of the output shaft of the sampling gun driving motor Structure, by the linear motion that the convert rotational motion of the sampling gun driving motor is the driving portion.

Optionally, the sampling gun transmission mechanism be screw-and-nut mechanism, including with the sampling gun driving motor Output axis connection and the first screw rod for being pivotally supported on the fixed pedestal and can be moved along the short transverse The first nut on first screw rod is arranged in ground, and the driving portion is the drive being set on the peripheral surface of first nut Baffle is moved, the holding tank on the head for the telescopic rod to be clamped is formed on the driving baffle.

Optionally, the magnetic bead transfer device includes the magnetic being arranged on the position in microscope carrier transport establishment close to sampling hammerhead Pearl transfer device fixed seat, the magnetic part actuator being arranged in the magnetic bead transfer device fixed seat and magnetic part movement Plate, magnetic part movable plate setting are connect in the magnetic bead transfer device fixed seat, and with the magnetic part actuator with energy Enough flexible along the direction closer or far from the sampling hammerhead, the magnetic part is magnet and is mounted on the magnetic part movable plate Close to the side of the sampling gun.

Optionally, the magnetic part actuator includes magnetic part driving motor and magnetic part bolt and nut mechanism, the magnetic Property part driving motor be arranged in the magnetic bead transfer device fixed seat, the magnetic part screw rod of magnetic part bolt and nut mechanism With the output axis connection of the magnetic part driving motor, magnetic part nut and the magnetic part of magnetic part bolt and nut mechanism Movable plate connects.

Optionally, the guiding of the telescopic direction extension along the magnetic part movable plate is formed on the magnetic part movable plate Slot hole is provided with the positioning screw for being inserted into and being installed in the long guiding hole, the magnetic on the magnetic bead transfer device Property part movable plate is telescopically disposed in the magnetic bead transfer device by the cooperation of the long guiding hole and the positioning screw On.

According to another aspect of the present disclosure, a kind of sample processing device is provided, the sample processing device includes microscope carrier fortune Transfer mechanism and the nucleic acid extraction mechanism as described above being arranged in the microscope carrier transport establishment.

According to the another aspect of the disclosure, a kind of nucleic acid extraction side using nucleic acid extraction mechanism as described above is provided Method, the method for extracting nucleic acid include：Cleavage step, the sampling hammerhead are drawn sample and are contained into the sample container The sample is added in the cell pyrolysis liquid of magnetic bead so that obtaining adsorption after sample cracking has the bead complexes of nucleic acid, institute It states sampling hammerhead and draws the solution containing bead complexes, in the institute that the magnetic part is stretched out close to the direction of the sampling hammerhead It states under magnetic state, the sampling hammerhead spues molten in addition to the bead complexes being adsorbed on the sampling hammerhead inner wall Liquid；Washing step, under the demagnetizing state that direction of the magnetic part far from the sampling hammerhead is retracted, the sampling hammerhead is inhaled It takes the cleaning solution in the sample container and executes compressing action repeatedly, to clean bead complexes, later described for magnetic Under state, the sampling hammerhead, which spues, removes the solution containing bead complexes；Elution step, it is described under the demagnetizing state Sampling hammerhead draws the eluent in the sample container and executes compressing action repeatedly, so that the magnetic in bead complexes Pearl and nucleic acid separation, the sampling hammerhead draw the solution containing magnetic bead and nucleic acid；Nucleic acid extraction step, described for magnetic state Under, the sampling hammerhead will be except the nucleic acid containing magnetic bead spues and preserves to the sample container, the nucleic acid extraction side Method in the sample container in step-by-step movement traveling process, the lifting action by the pipette tips mobile device and the magnetic bead The cooperation of the expanding-contracting action of transfer device is sequentially inserted into the holding tank of each sample container and executes the cracking Step, the washing step, the elution step and the nucleic acid extraction step and obtain nucleic acid.

Optionally, it in the cleavage step, after the sampling hammerhead draws the solution containing bead complexes, also wraps Protein digestibility step is included, under the demagnetizing state, the sampling hammerhead spues the solution containing bead complexes to institute Compressing action is stated in the protein digestibility enzyme solutions in sample container and executes repeatedly, with the protein in digestion solution, it Under afterwards described for magnetic state, the sampling hammerhead, which spues, removes the solution containing bead complexes, to complete the cracking step Suddenly.

Through the above technical solutions, that is, the nucleic acid extraction mechanism of the disclosure in microscope carrier transport establishment by being arranged pipette tips Mobile device and magnetic bead transfer device, wherein the magnetic part of magnetic bead transfer device is set as in microscope carrier transport establishment, Neng Gouyan It is flexible closer or far from the direction of the sampling hammerhead, thus during nucleic acid extraction, in each sample of sample container It accommodates and is useful in the state of each reaction reagent of extraction nucleic acid in holding tank, reacted in corresponding sample holding tank The bead complexes containing sample and magnetic bead afterwards follow sampling hammerhead to move always, therefore the nucleic acid extraction of each sample only needs It can be realized as by a sampling hammerhead, thereby save sampling hammerhead consumptive material, reduce nucleic acid extraction cost.And by Nucleic acid extraction operation is executed in the state of accommodating each reaction reagent in each sample holding tank, thus sampling hammerhead The solution after reacting in the correspondence sample holding tank of sample container need to be shifted, without being executed again as existing middle to each The action of the corresponding reaction reagent of injection in holding tank, to be integrally improved nucleic acid extraction operating efficiency.

Other feature and advantage of the disclosure will be described in detail in subsequent specific embodiment part.

Description of the drawings

Attached drawing is for providing further understanding of the disclosure, and a part for constitution instruction, with following tool Body embodiment is used to explain the disclosure together, but does not constitute the limitation to the disclosure.In the accompanying drawings：

Fig. 1 is the stereogram according to the nucleic acid extraction mechanism of disclosure specific implementation mode；

Fig. 2 is the master for being provided with pipette tips and ejecting the pipette tips mobile device of releasing mechanism according to disclosure specific implementation mode View；

Fig. 3 is the right view of Fig. 2；

Fig. 4 is the rearview of Fig. 2；

Fig. 5 is the front view that releasing mechanism is ejected according to the pipette tips of disclosure specific implementation mode；

Fig. 6 is the right view of Fig. 5；

Fig. 7 is the cutaway view Amplified image along F-F line cuttings in Fig. 6；

Fig. 8 is that the stereogram that sampling gun is removed in releasing mechanism is ejected according to the pipette tips of disclosure specific implementation mode；

Fig. 9 is the stereogram one according to the magnetic bead transfer device of disclosure specific implementation mode；

Figure 10 is the stereogram two according to the magnetic bead transfer device of disclosure specific implementation mode；

Figure 11 is the solid according to the sample processing device for being provided with nucleic acid extraction mechanism of disclosure specific implementation mode Figure；

Figure 12 is the step flow diagram according to the method for extracting nucleic acid of disclosure specific implementation mode.

Reference sign

1- microscope carriers transport establishment 2- microscope carriers, 3- sample containers, 4- nucleic acid extractions mechanism, 41- pipette tips mobile devices, 411- Pedestal, 412- releasing mechanism driving motors, 413- releasing mechanism screw-and-nut mechanisms, 414- releasing mechanism guide frames, 415- fixed pedestal position sensors, 42- magnetic bead transfer devices, 421- magnetic bead transfer device fixed seats, 4211- movable plates are led Rail, 422- magnetic part movable plates, 423- magnet, 424- long guiding holes, 425- positioning screws, 426- magnetic part driving motors, 427- magnetic part screw rods, 428- magnetic part nuts, 429- magnetic part movable plate position sensors,

5- pipette tips eject releasing mechanism, 51- sampling guns, 511- telescopic rods, 512- main bodys, 513- telescopic rods channel, 514- Pipette tips interconnecting piece, 515- pipette tips linkage sections, 516- seal sections, 517- locking protrusions, 52- sampling gun actuators, 521- fix base Seat, 5211- connecting rod mounting holes, 5212- axle sleeves, 5211- assembling stands, 522- sampling gun driving motors, the first screw rods of 523-, 524 first nuts, 525- driving baffles, 526- holding tanks, 527- vertical plates, 528- upper water tablets, 5281- guide rods, 5282- guideways, 5283- drive baffle position sensor, 529- lower horizontal plates, 5291- main body holding tanks, 53- ejections Release piece, 531- eject disengaging plate, and 532- pipette tips interconnecting pieces are inserted into hole, and 533- supports plate, 5331- telescopic rod holding tanks, 534- Connecting rod, 535- resetting springs,

200- sampling hammerhead Z2- nucleic acid extractions direction, X2- horizontal directions, H- short transverse S21- cleavage steps, S22- are washed Wash step, S23- elution steps, S24- nucleic acid extraction steps, S25- protein digestibility steps.

Specific implementation mode

The specific implementation mode of the disclosure is described in detail below in conjunction with attached drawing.It should be understood that this place is retouched The specific implementation mode stated is only used for describing and explaining the disclosure, is not limited to the disclosure.

In the disclosure, in the absence of explanation to the contrary, the noun of locality used such as " upper and lower, left and right " typically refers to " upper and lower, left and right " of corresponding component under use state, " inside and outside " they refer to " inside and outside " for corresponding component outer profile, This, " short transverse " referred in the disclosure refers to the upper and lower directions of nucleic acid extraction mechanism in a state of use i.e. such as Fig. 1 and Fig. 2 Shown in the directions H, " horizontal direction " refers to directions X2 as shown in Figure 4 and Figure 5, and " nucleic acid extraction direction " refers to as shown in figure 11 The directions Z2.

With reference first to Fig. 1 to Figure 11 to according to the structure of the nucleic acid extraction mechanism of disclosure specific implementation mode, feature with And function and effect are described.

As shown in Figure 1 to 11, the nucleic acid extraction mechanism of the disclosure utilizes the sample with multiple sample holding tanks to accommodate Device 3 extracts nucleic acid, and the nucleic acid extraction mechanism 4 is arranged in microscope carrier transport establishment 1, and includes：Pipette tips mobile device 41, pipette tips Mobile device 41 can be up and down arranged along short transverse H in microscope carrier transport establishment 1, separatably be arranged in rifle for driving Sampling hammerhead 200 on head moving device 41 enables to sampling hammerhead 200 to adsorb sample along short transverse H shift position The liquid in liquid or discharge sampling hammerhead 200 in container 3；Magnetic bead transfer device 42, the magnetic bead transfer device 42 include Magnetic part, magnetic part can be telescopically arranged along the direction closer or far from sampling hammerhead 200 in microscope carrier transport establishment 1, with So that magnetic part has acts on sampling hammerhead 200 to the magnetic bead offer magnetic force in sampling hammerhead 200 for magnetic state and removing The demagnetizing state of the magnetic force of interior magnetic bead.As described above, the nucleic acid extraction mechanism of the disclosure extracts nucleic acid using paramagnetic particle method, specifically Ground, during extracting nucleic acid, the nucleic acid for sample be released after cell cracking first is mixed with magnetic bead, is formed Nucleic acid-bead complexes, at this time can be by the decline of pipette tips mobile device 41 so that being loaded in pipette tips mobile device 41 Sampling hammerhead 200 adsorbs the bead complexes, shifts filling by the lifting action and magnetic bead of pipette tips mobile device 41 later 42 expanding-contracting actions towards the direction closer or far from sampling hammerhead 200 are set, which is transferred to sample respectively holds It receives in each sample holding tank of device 3 and executes and the operations such as correspondingly wash, elute.Specifically, in multiple sample holding tanks Reaction reagent (for example, cell pyrolysis liquid, cleaning solution, eluent etc.) between transfer nucleic acid-bead complexes when, nucleic acid-magnetic Pearl compound is placed in always in sampling hammerhead 200, and nucleic acid-magnetic bead is compound in the case where the offer of magnetic bead transfer device 42 is for magnetic state Object is adsorbed on the inner wall of sampling hammerhead 200, in this case by sampling hammerhead 200 waste liquid (such as complete cracking cracking Waste liquid, the scrub raffinate for completing washing, the elution waste liquid etc. for completing elution) it is expelled to the corresponding sample appearance of sample container 3 It receives in slot, internal nucleic acid-bead complexes are expelled to next sample appearance of sample container 3 by sampling hammerhead 200 later It receives in slot.And in existing sample extraction equipment, generally use mechanical arm moves in microscope carrier transport establishment, and microscope carrier and sample Container is relative to the fixed nucleic acid extraction mode of microscope carrier transport establishment, in the case, during nucleic acid extraction, pass through by Bead complexes are placed in always in the state of sample container, and waste liquid and injection in sample container are drawn by sampling hammerhead The mode of new reaction reagent executes nucleic acid extraction operation.Specifically, it is accommodated in nucleic acid-bead complexes and sample in existing After a certain reaction reagent in device is sufficiently mixed, magnetic force generation device provides magnetic force to sample container so that nucleic acid-magnetic bead is multiple It closes object to be adsorbed on sample container inner wall, at this point, mechanical arm driving sampling hammerhead draws the reaction reagent in sample container Waste liquid and exclude, while the sampling hammerhead that discarded use is crossed, later mechanical arm reload new sampling hammerhead by another Reaction reagent is injected into sample container and is mixed with nucleic acid-bead complexes, makees to execute nucleic acid extraction successively Industry.This sample processing device in existing is during nucleic acid extraction, since a reaction reagent is corresponded to using a sampling Pipette tips lead to the serious waste of sampling hammerhead, and hold each sample often through the mode of mobile magnetic force generation device Device of receiving is executed for magnetic or degaussing operation, or is respectively accordingly arranging that magnetic force generates dress close to the position of each sample container It sets, so that the nucleic acid extraction operation of sample processing device is increasingly complex, and the problem with manufacture and processing cost costliness. And for the nucleic acid extraction mechanism of the disclosure, nucleic acid extraction mechanism 4 in microscope carrier transport establishment 1 by being arranged pipette tips mobile device 41 and magnetic bead transfer device 42, wherein the magnetic part of magnetic bead transfer device 42 is set as in microscope carrier transport establishment 1, Neng Gouyan It is flexible closer or far from the direction of the sampling hammerhead 200, thus during nucleic acid extraction, in each of sample container 3 It accommodates and is useful in the state of each reaction reagent of extraction nucleic acid in sample holding tank, occurred in corresponding sample holding tank Bead complexes containing sample and magnetic bead after reaction follow sampling hammerhead 200 to move always, therefore the nucleic acid of each sample carries Take and only need to can be realized as by a sampling hammerhead 200, thereby save sampling hammerhead consumptive material, reduce nucleic acid extraction at Originally, nucleic acid extraction operation is executed in the state of and due to accommodating each reaction reagent in each sample holding tank, thus Solution after reacting in the correspondence sample holding tank that sampling hammerhead 200 need to shift sample container 3, without executing again Such as existing middle action that corresponding reaction reagent is injected into each holding tank, to be integrally improved nucleic acid extraction operation Efficiency.In addition, during nucleic acid extraction, due to pipette tips mobile device, 41 drive sampling hammerhead 200 to be moved along short transverse H Position, therefore can make magnetic bead transfer device 42 in the state of being relatively fixed in microscope carrier transport establishment 1, it is shifted by magnetic bead The expanding-contracting action of the magnetic part of device 42 and convenient for providing magnetic force or removal magnetic force to nucleic acid-bead complexes so that core Sour extraction operation is simplified, and manufacture and the processing cost of nucleic acid extraction mechanism are reduced.

Here, pipette tips mobile device 41 and magnetic bead transfer device 42 may be used the arrangement of various reasonable, example Such as, optionally, pipette tips mobile device 41 includes the pedestal 411 being arranged in microscope carrier transport establishment 1 and can be along the short transverse The ejection releasing mechanism 5 of the pipette tips on pedestal 411 is arranged in H up and down.Here, can be suitable for the rifle pipette tips mobile device 41 The crown goes out releasing mechanism 5 and is configured to such as lower structure, that is, as shown in Fig. 5 to Fig. 8, pipette tips ejection releasing mechanism 5 may include Sampling gun 51, sampling gun actuator 52 and ejection release piece 53 for installing sampling hammerhead 200, the drive of sampling gun actuator 52 Dynamic portion connect with the telescopic rod 511 of sampling gun 51 and for driving telescopic rod 511 flexible, so that sampling gun 51 is sampling hammerhead 200 provide the pressure of the suction of imbibition or drain, and ejection release piece 53 is mounted on sampling gun actuator 52 and enables to take Sample pipette tips 200 are detached with sampling gun 51.As described above, in the case where sampling hammerhead 200 is loaded into the use state of sampling gun 51, lead to The driving of the driving portion of over sampling rifle actuator 52, sampling gun 51 carry out expanding-contracting action and sampling hammerhead 200 are made to draw liquid Or discharge liquid, pipette tips 200 to be sampled can be by ejecting release piece 53 so that sampling hammerhead after completing nucleic acid extraction operation 200 easily fall out from sampling gun 51, improve the efficiency of loading and unloading of sampling hammerhead 200, thus, it is possible to nucleic acid extraction is effectively ensured The reliability of operation.

In addition, here, ejection release piece 53 could be provided as the structure of various reasonable, as long as can be by sampling hammerhead 200 apply the active force towards off-direction, realize the function that sampling hammerhead 200 is detached from from sampling gun 51.For example, ejection Release piece 53 can be arranged to have mobilizable clamping limb, clamp sampling hammerhead 200 by the clamping limb and by sampling hammerhead 200 are detached from from sampling gun 51, and for another example, optionally, as shown in figure 5 and figure 7, sampling gun 51 includes main body 512 and multiple telescopic rods 511, main body 512 is arranged on the fixed pedestal 521 of sampling gun actuator 52, and is opened with the both ends being inserted into for telescopic rod 511 Multiple telescopic rod channels 513 of mouth, the top of each telescopic rod 511 are connect with the driving portion of sampling gun actuator 52, and each Telescopic rod 511 can telescopically be inserted into corresponding telescopic rod channel 513 by the driving of driving portion along short transverse H Interior, the bottom corresponding to each telescopic rod channel 513 of main body 512 is respectively structured as connecting with corresponding sampling hammerhead 200 The pipette tips interconnecting piece 514 connect, ejection release piece 53 is configured to move relative to each pipette tips interconnecting piece 514, can make Each sampling hammerhead 200 is obtained to detach with corresponding pipette tips interconnecting piece 514.Wherein, multiple telescopic rods are formed in main body 512 Channel 513, here, each telescopic rod channel 513 with the close external part of corresponding telescopic rod 511 (highly under use state Top on the H of direction) part between for sealing cooperation, you can by by arrange sealing ring in a manner of realize telescopic rod channel Sealing cooperation between 513 and telescopic rod 511, with when telescopic rod 511 does extending action in telescopic rod channel 513, due to Area of low pressure is formed in telescopic rod channel 513, liquid can pass through the liquid sucting port of sampling hammerhead 200 under external atmosphere pressure effect It enters in sampling hammerhead 200.The operation principle of this sampling gun 51 is similar with the operation principle of syringe.In addition, pipette tips connect Socket part 514 can be formed as the arrangement of various reasonable, for example, to may be integrally formed at main body 512 right for pipette tips interconnecting piece 514 Should be in the bottom in telescopic rod channel 513, alternatively, for the ease of the structure of machine-shaping sampling gun 51, pipette tips interconnecting piece 514 also may be used To form the bottom for being independent component and being connected to main body 512 corresponding to telescopic rod channel 513.Pass through structure as described above Pipette tips eject releasing mechanism 5, you can with by multiple sampling hammerheads 200 and with the sample container 3 of multigroup sample holding tank Cooperation, operations such as synchronous nucleic acid extraction for executing multiple samples, and by ejecting release piece 53 so that multiple sampling hammerheads 200 disposably fall off from each pipette tips interconnecting piece 514 simultaneously, and the dismounting for thus effectively saving sampling hammerhead 200 is taken Between, nucleic acid extraction efficiency equal samples treatment effeciency can be significantly improved.

Optionally, as shown in Figure 6 and Figure 8, ejection release piece 53 includes that can be up and down supported on along the short transverse H Ejection disengaging plate 531 on the fixed pedestal 521 of sampling gun actuator 52, the ejection disengaging plate 531 is in the short transverse H Positioned at the lower section of main body 512, and ejects and be formed with multiple pipette tips interconnecting pieces insertions hole 532 on disengaging plate 531, each pipette tips connection Respectively accordingly perforation pipette tips interconnecting piece is inserted into hole 532 and is exposed to ejection disengaging plate 531 the pipette tips linkage section 515 in portion 514 The driving portion of side, sampling gun actuator 52 is set as, and under the retracted mode that telescopic rod 511 is retracted, ejection can be driven de- The sampling hammerhead 200 on each pipette tips interconnecting piece 514 is squeezed along the off-direction of sampling hammerhead 200 from plate 531.That is, in order to Ensure more efficiently to unload sampling hammerhead 200 in the state that sampling hammerhead 200 empties liquid, ejection disengaging plate 531 is located at Make motion path smallizationer adjacent to the position of sampling hammerhead 200 and act rapidly on sampling hammerhead 200, specifically, takes Sample pipette tips 200 are mounted on the part for the side for being exposed to ejection disengaging plate 531, are driven in the driving portion of sampling gun actuator 52 In the state that dynamic telescopic rod 511 is fully retracted, ejection disengaging plate 531 can be further driven to be moved along off-direction so that top Go out disengaging plate 531 to be in contact with each sampling hammerhead 200 and push each sampling hammerhead 200 along off-direction, it is thus each Sampling hammerhead 200 can fall off from each pipette tips interconnecting piece 514 simultaneously.Pipette tips top is enabled to by structure as described above The integral arrangement for going out releasing mechanism 5 is more rationalized.In addition, since the telescopic rod for driving sampling gun 51 is utilized The driving source that 511 flexible sampling gun actuators 52 are moved as driving ejection disengaging plate 531, there is no need to individually arrangement is used again In the independent driving source that driving ejection disengaging plate 531 moves, saves manufacturing cost and disclosure satisfy that so that pipette tips ejection is detached from The requirement of overall structure simplerization of mechanism 5.

Here, in order to ensure that the driving portion of sampling gun actuator 52 steadily and reliably drives ejection disengaging plate 531 to move And be in contact with each sampling hammerhead 200, and make pipette tips ejection releasing mechanism 5 each structure arrangement it is compacter and Rationally, optionally, as shown in figure 8, the ejection release piece 53 includes supporting plate 533 and being detached from supporting plate 533 and ejecting The connecting rod 534 that plate 531 connects, supports plate 533 and is located between the driving portion and main body 512 of sampling gun actuator 52, support plate 533 can be up and down arranged along the short transverse H on fixed pedestal 521 with ejection disengaging plate 531 by connecting rod 534, Driving portion can abut under the retracted mode with plate 533 is supported, to push ejection release piece 53 to be moved along the off-direction Dynamic, connecting rod 534 is resiliently supported at by the resetting spring 535 being set in the connecting rod 534 on fixed pedestal 521, is resetted The both ends of spring 535 respectively with support plate 533 and fixed pedestal 521 abuts, drive ejection release piece 53 always for providing Return back to the elastic-restoring force of initial position.Wherein, it can be provided with connecting rod mounting hole 5211 on fixed pedestal 521, connect Bar 534 is by the respective outer side edges such as axle sleeve 5212 to connecting rod mounting hole 5211, in 534 lifting process bottom bracket axle of connecting rod The structures such as 5212 play the role of protecting connecting rod 534 and effectivelying prevent connecting rod 534 that movement abrasion occurs.Wherein, in order to just The all parts of releasing mechanism 5 are ejected in assembly pipette tips, and make the structure of ejection release piece 53 and sampling gun 51 arrangement more Adduction physics and chemistry optionally supports the telescopic rod holding tank 5331 that could be formed on plate 533 and passed through for each telescopic rod 511, This, under the original state that ejection release piece 53 is in idle, the elastic anchorage force provided by resetting spring 535 to eject Release piece 53 is supported integrally on fixed pedestal 521, in the case where ejection release piece 53 is in running order, sampling gun actuator 52 It driving portion and supports plate 533 and is in contact and so that ejection is de- by connecting rod 534 is in the guiding role on fixed pedestal 521 Off member 53 is whole (i.e. in short transverse H downwards) mobile towards off-direction, in the process, ejection disengaging plate 531 with respectively A sampling hammerhead 200 is in contact and pushes each sampling hammerhead 200 that it is made to be connected from the pipette tips of corresponding sampling gun 51 Portion 514 falls off.In addition, after the unloading for completing sampling hammerhead 200, removed in sampling gun actuator 52 to supporting plate 533 When active force, ejection release piece 53 is returned to idle initial position entirely through the elastic-restoring force of resetting spring 535.By This, by structure as described above can handling operation that is accurate and fast implementing sampling hammerhead 200, nucleic acid extraction is effectively ensured Operation normal operation.But it's not limited to that for the disclosure, and the concrete structure for ejecting release piece 53 can be according to sample process The actual arrangement structure of equipment carrys out reasonable design, for example, the type of drive for ejecting release piece 53, can be used alone another Outer driving source and do not generated with sampling gun actuator 52 and linkedly directly drive the movement of ejection release piece 53 and so that ejection is de- Off member 53 coordinates with sampling hammerhead 200 and realizes unloading of the sampling hammerhead 200 from pipette tips interconnecting piece 514.

Here, in order to further increase nucleic acid extraction equal samples treatment effeciency, optionally, as shown in Figure 1,51 edge of sampling gun Horizontal direction X2 be positioned apart from it is multiple, support plate 533 and ejection disengaging plate 531 respectively in transverse direction X2 extend, connection Bar 534 is multiple and the intervals X2 are arranged there are one the mode of sampling gun 51 and are supporting plate 533 and ejection is de- in transverse direction respectively From between plate 531, distinguish between plate 533, ejection disengaging plate 531 and every adjacent each two connecting rod 534 so that supporting Sampling gun layout area is constituted, the main body 512 of each sampling gun 51 is located in the sampling gun layout area.Wherein, Ge Gelian It can be arranged with resetting spring 535 as described above on extension bar 534, enhance and 53 whole resilient support of ejection release piece is imitated Fruit improves ejection by multiple connecting rods 534 so that supporting plate 533 and ejection disengaging plate 531 is firmly connected as overall structure The whole bonding strength of release piece 53 and rigidity, effectively avoid occuring bending and deformation during pushing multiple sampling hammerheads Phenomenon.In addition, in the specific implementation mode of the disclosure, for example, sampling gun 51 can be positioned apart from four with X2 in transverse direction It is a, and there are four telescopic rod channel 513 and four telescopic rods 511 for setting in each sampling gun 51, it can be by as described above Four sampling guns 51 and the nucleic acid extraction equal samples processing operation that disposably can synchronously execute 16 samples, and pass through The disposable synchronously execution unloading of 16 sampling hammerheads 200 is enabled to make using plate 533 and ejection disengaging plate 531 is supported Industry so that ejection release piece 53 is more simple for the unloading operation of sampling hammerhead 200 and is easily achieved.

Here, optionally, as shown in fig. 7, each pipette tips interconnecting piece 514 includes the bottom end opening with telescopic rod channel 513 The seal section 516 for sealing cooperation, the pipette tips linkage section 515 to connect with the seal section 516, being interval in for pipette tips linkage section 515 are close The locking protrusion 517 being formed on the position of section 516 for being engaged by clamping with sampling hammerhead 200 is sealed, ejection disengaging plate 531 is located at Between seal section 516 and locking protrusion 517, locking protrusion 517 is arranged to be inserted into hole 532 across pipette tips interconnecting piece.Here, The main body 512 of pipette tips interconnecting piece 514 and sampling gun 51, which is arranged to separate structure, to be ejected for the ease of processing and assembling pipette tips The all parts of releasing mechanism 5, and also have the effect of convenient for follow-up maintenance and reduce maintenance cost.In addition, that will sample When pipette tips 200 are loaded into pipette tips linkage section 515, since the locking protrusion 517 on pipette tips linkage section 515 is sent out with sampling hammerhead 200 It is raw to be engaged by clamping, to ensure that sampling hammerhead 200 holds normal imbibition and draining function, in nucleic acid extraction equal samples processing procedure In avoid there is a phenomenon where sampling hammerhead 200 from pipette tips linkage section 515 be detached from.

Optionally, as shown in figure 8, sampling gun actuator 52 includes fixed pedestal 521, is arranged on the fixed pedestal 521 Sampling gun driving motor 522, with the transmission of the output shaft of sampling gun driving motor 522 be connected and be provided with the sampling gun of driving portion Transmission mechanism, using by the convert rotational motion of sampling gun driving motor 522 as the linear motion of driving portion.Wherein, fixed pedestal 521 can be arranged on the pedestal 411 of pipette tips mobile device 41, and the arrangement knot of various reasonable may be used in sampling gun transmission mechanism Structure, as long as can realize the function for the linear motion that the convert rotational motion of sampling gun driving motor 522 is driving portion, For example, sampling gun transmission mechanism can be rack pinion matching mechanism, Worm Wheel System matching mechanism, worm drive machine Structure etc..Here, optionally, sampling gun transmission mechanism is screw-and-nut mechanism, includes defeated with sampling gun driving motor 522 Go out axis connection and the first screw rod 523 for being pivotally supported on fixed pedestal 521 and can movably be set along short transverse H The first nut 524 on the first screw rod 523 is set, driving portion is the driving baffle being set on the peripheral surface of the first nut 524 525, the holding tank 526 on the head for telescopic rod 511 to be clamped is formed on the driving baffle 525.Here, the first screw rod 523 It can be pivotally supported at by bearing on fixed pedestal 521, here, for the ease of the first nut of connection 524 and driving gear Plate 525, the first nut 524 can be formed as T-nut, and driving baffle 525 is set on the small head end of the T-nut and passes through Bolt and the stub end of T-nut are fixed together, and the connection of driving baffle 525 and the first nut 524 has thus been effectively ensured Reliability.In addition, in the case where sampling gun is provided with multiple, X2 extends and can be formed driving baffle 525 in transverse direction For with the development length roughly the same with the development length for supporting plate 533, to enable to driving baffle 525 to supporting plate 533 apply uniform power, are effectively ensured to support plate 533, connecting rod 534 and eject 531 entirety of disengaging plate and be put down along short transverse H Quietly move.In addition, for the ease of the telescopic rod 511 of sampling gun 51 to be assembled on driving baffle 525, optionally, holding tank 526 are formed to have the C-channel of opening, and the head of telescopic rod 511 circumferentially could be formed with the inside for snapping fit onto C-channel Clamping engagement groove on wall, thus telescopic rod 511 is by snapping fit onto in C-channel clamping engagement slot from the opening of C-channel, by While this ensures telescopic rod 511 and drives the assembly reliability between baffle 525, realize to telescopic rod 511 relative to drive Dynamic limit of the baffle 525 in short transverse H.Have by using screw-and-nut mechanism as described above and matches occlusal wear Small, transmission efficiency, stable drive, long lifespan and advantage with high accuracy.

The lightweight of releasing mechanism 5 and the demand of saving manufacturing cost, optionally, fixed base are ejected in order to meet pipette tips Seat 521 is including vertical plate 527 and along short transverse H arranged for interval in the upper water tablet 528 of the side of vertical plate 527 and lower part Level board 529, sampling gun driving motor 522 and sampling gun transmission mechanism are arranged on upper water tablet 528, eject release piece 53 Can up and down it be arranged on lower horizontal plate 529 along short transverse H, driving baffle 525 is located at upper water tablet 528 and lower part Between level board 529.Wherein, main body holding tank 5291, the main body 512 of sampling gun 51 are could be formed on lower horizontal plate 528 It can be received into the main body holding tank 5291 and be fixed on lower horizontal plate 528 by fasteners such as bolts, be therefore saved on Pipette tips eject the arrangement space of releasing mechanism 5.In addition, using bolt and nut drive mechanism as described above, take Sample rifle driving motor 522 is mounted on the top of upper water tablet 528, the output of the first screw rod 523 and sampling gun driving motor 522 Axis connection simultaneously is arranged as penetrating through upper water tablet 528 and driving baffle 525 successively along short transverse H, here, in order to which stabilization is led To driving the moving in short transverse H of baffle 525, optionally, sampling gun driving motor is located in upper water tablet 528 It can be provided with the guide rod 5281 of perforation driving baffle 525 on the position of 522 both sides, drive baffle 525 and guide rod Guideway 5282 is provided between 5281.In addition, being also provided on upper water tablet 528 for detecting driving baffle The baffle position sensor 5283 of 525 position, for example, as shown in figure, baffle position sensor 5283 can be photoelectric transfer Groove profile photoelectrical position sensor etc. specifically may be used in sensor, here, baffle position sensor 5283 can for example detect High limit position of the baffle 525 in short transverse H is driven, whether to detect the telescopic rod 511 of sampling gun 51 in complete Stretching state.Correspondingly, it can also be provided on lower horizontal plate 529 for detecting driving baffle 525 in short transverse H Most sole extreme position position sensor, with detect sampling gun 51 telescopic rod whether be in fully retracted state.

When pipette tips as described above ejection releasing mechanism is adapted to pipette tips mobile device 41 as described above, such as Fig. 1 It, can be by enabling pipette tips to eject releasing mechanism 5 along height side fixed pedestal 521 as described above to as shown in Figure 4 It is arranged up and down on pedestal 411 to H, here, for example, pedestal 411 can be formed as gantry shape, at the top of pedestal 411 Releasing mechanism driving motor 412 is set, which passes through releasing mechanism screw-and-nut mechanism 413 It is connected with the transmission of fixed pedestal 521, that is, the screw rod of releasing mechanism screw-and-nut mechanism 413 extends and passes through along short transverse H The assembling stand 5211 of logical fixed pedestal 521, here, being oriented to pipette tips ejection (the specially assembling stand of releasing mechanism 5 to play to stablize 5211) moving along short transverse H can be located at the two of releasing mechanism driving motor 412 in pedestal 411 as illustrated in fig. 4 The releasing mechanism guide frame 414 that setting ejects the movement of releasing mechanism 5 for being oriented to pipette tips on side position.The releasing mechanism is led Identical as guide rod 5281 as described above and the structure of guideway 5282 to structure 414, details are not described herein.In addition, can To be provided with the fixed pedestal position sensor 415 of the position for detecting fixed pedestal 521 on pedestal 1, thus, it is possible to go out Detect high limit position of the pipette tips ejection releasing mechanism 5 in short transverse H.

Optionally, as shown in Fig. 1, Fig. 9 and Figure 10, the magnetic bead transfer device 42 in nucleic acid extraction mechanism 4 as described above wraps It includes and magnetic bead transfer device fixed seat 421 in microscope carrier transport establishment 1 on the position of sampling hammerhead 200 is set, is arranged in magnetic Magnetic part actuator in pearl transfer device fixed seat 421 and magnetic part movable plate 422, the magnetic part movable plate 422 setting In magnetic bead transfer device fixed seat 421, and it connect with magnetic part actuator with can be along closer or far from sampling hammerhead 200 Direction is flexible, and magnetic part is magnet 423 and is mounted on magnetic part movable plate 422 close to the side of sampling gun 51.Here, by as above In the case that the nucleic acid extraction mechanism 4 is suitable for sample processing device, the magnetic bead transfer device of magnetic bead transfer device 42 is solid Reservation 421 can be arranged in the rear side i.e. downstream side for being located at pipette tips mobile device 41 on the Z2 of nucleic acid extraction direction, be moved in pipette tips In the case of being provided with multiple sampling guns 51 on the pipette tips ejection releasing mechanism 5 of dynamic device 41, magnet 423 is in magnetic part movable plate Can also be positioned apart from 422 position it is multiple, wherein the sampling gun loaded on each magnet 423 and each sampling gun 51 First 200 is opposite, can receive the effect of magnetic field force can effectively ensure that each sampling hammerhead 200, and then ensure nucleic acid Extract quality.Wherein, magnetic part actuator is adopted as the structure of various reasonable, for example, magnetic part actuator can be telescoping cylinder, To drive magnetic part movable plate 422 flexible towards the direction closer or far from sampling hammerhead 200, or, optionally, magnetic part Actuator includes magnetic part driving motor 426 and magnetic part bolt and nut mechanism, and the setting of magnetic part driving motor 426 turns in magnetic bead In moving device fixed seat 421, the magnetic part screw rod 427 of magnetic part bolt and nut mechanism is defeated with the magnetic part driving motor 426 Go out axis connection, the magnetic part nut 428 of magnetic part bolt and nut mechanism is connect with magnetic part movable plate 422.As a result, as described above Magnetic part actuator simple in structure has the characteristics that stable drive, use reliability are high.

In addition, pipette tips mobile device 41 as described above and magnetic bead transfer device 42 are suitable for sample as described above In the case of processing equipment, when moving to realize nucleic acid extraction operation along the step-by-step movement of nucleic acid extraction direction Z2 by microscope carrier 2, magnetic Pearl transfer device 42 can be located at the downstream side of magnetic bead transfer device 42 on the Z2 of nucleic acid extraction direction, to avoid microscope carrier 2 along core The movement of acid extraction direction Z2, at this point, since microscope carrier 2 is directed away from the direction of magnetic bead transfer device 42 during nucleic acid extraction Movement, therefore, magnetic bead transfer device 42 can (such as can be according to microscope carrier 2 according to the actual range between sampling hammerhead 200 Displacement distance) adjust the degree stretched out towards sampling hammerhead 200 in real time, be effectively ensured and provide magnetic to sampling hammerhead 200 Power or the overall distance that Z2 is moved along nucleic acid extraction direction during nucleic acid extraction of microscope carrier 2 are smaller, and make to sampling gun Under the premise of first 200 magnetic force applied have substantially no effect on absorption magnetic bead, the magnetic part of magnetic bead transfer device 42 can be made to carry Flexible degree is repeated in platform transport establishment 1 keeps identical.

Here, moved in order to being steadily oriented to magnetic part movable plate 422, and convenient for assembly magnetic part movable plate 422 and magnetic bead device device fixed seat 421 optionally as shown in Figure 10, be formed with along magnetic part on magnetic part movable plate 422 The long guiding hole 424 that the telescopic direction of movable plate 422 extends is provided on magnetic bead transfer device 42 and is led for being inserted into and being installed to Positioning screw 425 into slot hole 424, magnetic part movable plate 422 can by the cooperation of long guiding hole 424 and positioning screw 425 Telescopically it is arranged on magnetic bead transfer device 42.Here, mobile panel guide can be provided in magnetic bead transfer device fixed seat 421 4211, magnetic part movable plate 422 can play dual guiding role by the cooperation of sliding block and mobile panel guide 4211.? This can be provided with to accurately control the stretching displacement of magnetic part movable plate 422 in magnetic bead transfer device fixed seat 421 The magnetic part movable plate position sensor such as photoelectrical position sensor for the extended position for detecting magnetic part movable plate 422 429。

In addition, according to another aspect of the present disclosure, as shown in figure 11, the disclosure also provides a kind of sample processing device, it should Nucleic acid processing equipment includes microscope carrier transport establishment 1 and the nucleic acid extraction machine as described above that is arranged in the microscope carrier transport establishment 1 Structure.Wherein, the sample processing device of the disclosure can be used for detecting the nucleic acid for representing life entity hereditary feature basic unit, example Such as, the sample processing device of the disclosure can be applied to the detect and diagnose of the infectious diseases such as hepatitis, AIDS, influenza, hand-foot-and-mouth disease, It can be used for detecting and diagnosing Other diseases, pass through the sample processing device of the disclosure such as lower structure, that is, as above by being arranged The cooperation of the lifting action of the pipette tips mobile device 41 of the structure and the expanding-contracting action of magnetic bead transfer device 42, as described above The mode that bead complexes follow sampling hammerhead 200 to move always executes nucleic acid extraction operation, and due to the nucleic acid of each sample Extraction only need to can be realized as by a sampling hammerhead, thereby save sampling hammerhead consumptive material, reduce nucleic acid extraction at This.In addition, due to the nucleic acid extraction mechanism 4 be fixed on microscope carrier transport establishment 1 working region in stationary state under, pass through Sample container 3 is moved to execute the nucleic acid extractions operation such as cell cracking, washing, elution successively, thus nucleic acid easy to operation carries Take mechanism 4 so that the entire control mode of operation of sample processing device tends to simplify, is more easily implemented automation control.

In addition, being based on nucleic acid extraction mechanism 4 as described above, as shown in figure 12, the disclosure also provides a kind of using as above The method for extracting nucleic acid of the nucleic acid extraction mechanism 4, the method for extracting nucleic acid include：Cleavage step S21, sampling hammerhead The sample is simultaneously added into sample container 3 for 200 absorption samples in the cell pyrolysis liquid containing magnetic bead so that obtained after sample cracking Adsorption has the bead complexes of nucleic acid, sampling hammerhead to draw the solution containing bead complexes, in magnetic part close to taking The directions of sample pipette tips 200 stretch out under magnetic state, sampling hammerhead 200 spues except the magnetic being adsorbed on 200 inner wall of sampling hammerhead Solution except pearl compound；Washing step S22, under the demagnetizing state that direction of the magnetic part far from sampling hammerhead 200 is retracted, Sampling hammerhead 200 draws the cleaning solution in sample container 3 and executes compressing action repeatedly, to clean bead complexes, later Under for magnetic state, sampling hammerhead 200, which spues, removes the solution containing bead complexes；Elution step S23, under demagnetizing state, Sampling hammerhead 200 draws the eluent in sample container 3 and executes compressing action repeatedly, so that the magnetic in bead complexes Pearl and nucleic acid separation, sampling hammerhead 200 draw the solution containing magnetic bead and nucleic acid；Nucleic acid extraction step S24, for magnetic state Under, sampling hammerhead 200 is by except the nucleic acid containing magnetic bead spues and preserves to sample container 3, and method for extracting nucleic acid is in sample Container 3 is in step-by-step movement traveling process, to pass through the lifting action of pipette tips mobile device 41 and stretching for magnetic bead transfer device 42 The cooperation of action, be sequentially inserted into the holding tank of each sample container 3 and execute cleavage step S21, washing step S22, Elution step S23 and nucleic acid extraction step S24 and obtain nucleic acid.

Here, the process of nucleic acid extraction for convenience of description, by each sample holding tank of sample container 3 be defined as Under, that is, it will respectively be defined along the sample holding tank in contrast to nucleic acid extraction direction Z2 arrangements for accommodating cell pyrolysis liquid Cell pyrolysis liquid holding tank, the cleaning solution holding tank for accommodating cleaning solution, for accommodate the eluent holding tank of eluent with And the nucleic acid holding tank of the nucleic acid for accommodating extraction.As a result, when sample container 3 is moved along nucleic acid extraction direction Z2, rifle The sampling hammerhead 200 loaded on head moving device 41 can be sequentially inserted into cell pyrolysis liquid holding tank, cleaning solution holding tank, wash De- liquid holding tank and nucleic acid holding tank.

As described above, the method for extracting nucleic acid of the disclosure extracts nucleic acid using paramagnetic particle method, can realize high-throughput operation and Automation, and since paramagnetic particle method nucleic acid extraction has used inorganic particle to be combined the high-affinity to be formed with high molecular material Composite magnetic microballoon (magnetic bead), the composite magnetic microballoon have both numerous characteristics of polymer microsphere and magnetic particle, therefore are not having Having can be uniformly dispersed in the case of applying magnetic field, stablize in the solution, can be simple and quick in the case where applying magnetic field Ground is detached with solution, so that the nucleic acid purity extracted is high, dense with the specific binding calculated from there through composite magnetic microballoon Degree is big, and has the advantages that safe, of low cost and be convenient for extensive use.

In the method for extracting nucleic acid of the disclosure, in sample container 3 in step-by-step movement traveling process, to be moved and be filled by pipette tips The cooperation for setting 41 lifting action and the expanding-contracting action of magnetic bead transfer device 42 is sequentially inserted into the appearance of each sample container 3 It receives in slot and executes the nucleic acid extraction operation of interdependent ground.Specifically, the state of sampling hammerhead 200 is loaded in pipette tips mobile device 41 Under, sampling hammerhead 200 declines after drawing sample along short transverse H and be inserted into sample container 3 accommodates cell pyrolysis liquid In the cell pyrolysis liquid holding tank of magnetic bead, sample is expelled in cell pyrolysis liquid holding tank and executes and split by sampling hammerhead 200 Solve step S21.In cleavage step S21, sampling hammerhead 200 executes compressing action so that sample and cell pyrolysis liquid repeatedly It is sufficiently mixed, quiet to put a period of time so that fully dissolving occurs for the cell of sample, later, sample is released after carrying out cell cracking The nucleic acid gone out is mixed with magnetic bead, forms the solution containing nucleic acid-bead complexes (hereinafter referred to as bead complexes).Sampling Pipette tips 200 draw the cracking waste liquid containing bead complexes, in the case where magnetic part is for magnetic state, nucleic acid-bead complexes It can be adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device 41 drives the discharge of sampling hammerhead 200 except magnetic bead is compound Waste reaction solution except object.After completing cleavage step S21, pipette tips mobile device 41 drives sampling hammerhead 200 to rise and take off From cell pyrolysis liquid holding tank, sample container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement later so that sample container The 3 cleaning solution holding tank for accommodating cleaning solution is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives sampling gun First 200 decline and are inserted into cleaning solution holding tank, execute washing step S22.In washing step S22, in magnetic part Under demagnetizing state, sampling hammerhead 200 executes compressing action so that completing bead complexes and the washing of cleavage step S21 repeatedly Liquid is sufficiently mixed, and achievees the effect that thoroughly to clean impurity.Later, it is useless to draw the washing containing bead complexes for sampling hammerhead 200 Liquid, in the case where magnetic part is for magnetic state, bead complexes are adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device The 41 driving discharges of sampling hammerhead 200 are in the scrub raffinate to cleaning solution holding tank in addition to bead complexes.Here, for magnetic bead The washing step S22 of compound can be executed repeatedly, such as twice repeatedly, in the case, can also be right in sample container 3 It is provided with multiple such as two cleaning solution holding tanks with answering.After completing to the washing step S22 of bead complexes, pipette tips are moved Dynamic device 41 drives sampling hammerhead 200 to rise and be detached from cleaning solution holding tank, and sample container 3 is along nucleic acid extraction direction Z2 later Moved with step-by-step movement so that the eluent holding tank for accommodating eluent of sample container 3 be located at sampling hammerhead 200 just under Side, pipette tips mobile device 41 drive sampling hammerhead 200 to decline and are inserted into eluent holding tank, execute elution step S23.? In elution step S23, under the demagnetizing state of magnetic part, sampling hammerhead 200 executes compressing action so that completing to wash repeatedly The bead complexes and eluent for washing step S22 are sufficiently mixed, and achieve the effect that the extracting nucleic acid from magnetic bead.Sampling hammerhead 200 Draw the solution containing magnetic bead and nucleic acid.After completing to execute elution step S23, pipette tips mobile device 41 drives sampling hammerhead 200 rise and are detached from eluent holding tank, and sample container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement later so that sample The nucleic acid holding tank of this container 3 is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives under sampling hammerhead 200 It drops and is inserted into nucleic acid holding tank, execute nucleic acid extraction step S24.In nucleic acid extraction step S24, in the confession of magnetic part Under magnetic state, the discharge of sampling hammerhead 200 is in the nucleic acid solution to nucleic acid holding tank in addition to magnetic bead.Thus entire nucleic acid is completed to carry It is taken as industry.

To sum up, by method for extracting nucleic acid as described above during nucleic acid extraction, nucleic acid-magnetic bead as described above is multiple Close object follows sampling hammerhead 200 to move always, therefore the nucleic acid extraction of each sample is only needed through a sampling hammerhead energy It is enough to realize, sampling hammerhead consumptive material is thereby saved, nucleic acid extraction cost is reduced.In addition, during nucleic acid extraction, due to rifle Head moving device 41 drives sampling hammerhead 200 along short transverse H shift position, therefore magnetic bead transfer device 42 can be made to exist In the state of being relatively fixed in microscope carrier transport establishment 1, by the expanding-contracting action of the magnetic part of magnetic bead transfer device 42 convenient for Bead complexes provide magnetic force or removal magnetic force so that nucleic acid extraction simple operation, reduce nucleic acid extraction mechanism with And manufacture and the processing cost of sample processing device.

Optionally, in cleavage step S21, after sampling hammerhead 200 draws the solution containing bead complexes, further include Protein digestibility step S25, under demagnetizing state, sampling hammerhead 200 spues the solution containing bead complexes to the sample Compressing action is executed in protein digestibility enzyme solutions in this container 3 and repeatedly, with the protein in digestion solution, Zhi Hou The solution containing bead complexes is removed under magnetic state, the sampling hammerhead 200 spues, to complete the cleavage step S21.

Here, can be provided between cell pyrolysis liquid holding tank and cleaning solution holding tank for holding in sample container It receives the protein digestibility enzyme solutions holding tank containing protein kinase, splitting containing bead complexes is accommodated in sampling hammerhead 200 In the state of liquid waste solution, pipette tips mobile device 41 drives sampling hammerhead 200 to rise and be detached from cell pyrolysis liquid holding tank, later sample This container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement so that sample container 3 accommodates the egg containing protein kinase White matter digestion enzyme solutions holding tank is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives sampling hammerhead 200 to decline And be inserted into protein digestibility enzyme solutions holding tank, execute protein digestibility step S25.In protein digestibility step S25 In, under the demagnetizing state of magnetic part, sampling hammerhead 200 executes compressing action so that containing the magnetic for completing cell cracking repeatedly The cracking waste liquid of pearl compound is sufficiently mixed with the protein digestibility enzyme solutions containing protein kinase, reaches the egg in digestion solution The effect of white matter.Later, sampling hammerhead 200 draws the waste reaction solution containing bead complexes, in the case where magnetic part is for magnetic state, Bead complexes are adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device 41 drives the discharge of sampling hammerhead 200 to remove In waste reaction solution to protein digestibility enzyme solutions holding tank except bead complexes, executed to complete cleavage step S21 Next step, that is, washing step S22.By step as described above, nucleic acid extraction quality can be further increased, ensures sample The accuracy of processing.

The preferred embodiment of the disclosure is described in detail above in association with attached drawing, still, the disclosure is not limited to above-mentioned reality The detail in mode is applied, in the range of the technology design of the disclosure, a variety of letters can be carried out to the technical solution of the disclosure Monotropic type, these simple variants belong to the protection domain of the disclosure.

It is further to note that specific technical features described in the above specific embodiments, in not lance In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the disclosure to it is various can The combination of energy no longer separately illustrates.

In addition, arbitrary combination can also be carried out between a variety of different embodiments of the disclosure, as long as it is without prejudice to originally Disclosed thought equally should be considered as disclosure disclosure of that.

Claims

1. a kind of nucleic acid extraction mechanism, which is characterized in that extract core using the sample container (3) with multiple sample holding tanks Acid, the nucleic acid extraction mechanism (4) is arranged on the microscope carrier transport establishment (1), and includes：

Pipette tips mobile device (41), the pipette tips mobile device (41) can be up and down arranged along short transverse (H) in the microscope carrier In transport establishment (1), sampling hammerhead (200) edge on the pipette tips mobile device (41) is separatably set for driving The shift position short transverse (H), and sampling hammerhead (200) is enabled to adsorb liquid or row in the sample container (3) Put the liquid in the sampling hammerhead (200)；

Magnetic bead transfer device (42), which includes magnetic part, the magnetic part can along closer or far from The direction of the sampling hammerhead (200) is telescopically arranged on the microscope carrier transport establishment (1), so that the magnetic part has The magnetic bead of magnetic force acted on for magnetic state and removing in sampling hammerhead (200) is provided the magnetic bead in sampling hammerhead (200) The demagnetizing state of magnetic force.

2. nucleic acid extraction mechanism according to claim 1, which is characterized in that the pipette tips mobile device (41) includes setting Pedestal (411) on the microscope carrier transport establishment (1) and can up and down it be arranged in the pedestal along the short transverse (H) (411) the pipette tips ejection releasing mechanism (5) on, it includes for installing sampling hammerhead (200) which, which ejects releasing mechanism (5), Sampling gun (51), sampling gun actuator (52) and ejection release piece (53), the driving portion of the sampling gun actuator (52) and institute The telescopic rod (511) for stating sampling gun (51) connects and for driving the telescopic rod (511) flexible, so that the sampling gun (51) it is that the sampling hammerhead (200) provides the suction of imbibition or the pressure of drain, the ejection release piece (53) is mounted on institute It states on sampling gun actuator (52) and the sampling hammerhead (200) is enabled to be detached with the sampling gun (51).

3. nucleic acid extraction mechanism according to claim 2, which is characterized in that the sampling gun (51) includes main body (512) With multiple telescopic rods (511), fixed pedestal (521) of main body (512) setting in the sampling gun actuator (52) On, and with the multiple telescopic rod channels (513) for the both ends open being inserted into for the telescopic rod (511), each telescopic rod (511) top is connect with the driving portion of the sampling gun actuator (52), and each telescopic rod (511) passes through the drive The driving in dynamic portion and can be telescopically inserted into along short transverse (H) in corresponding telescopic rod channel (513), it is described The bottom corresponding to each telescopic rod channel (513) of main body (512) be respectively structured as with the corresponding sampling The pipette tips interconnecting piece (514) of pipette tips (200) connection, the ejection release piece (53) are configured to relative to each pipette tips Interconnecting piece (514) moves, to enable to each sampling hammerhead (200) and corresponding pipette tips interconnecting piece (514) it detaches.

4. nucleic acid extraction mechanism according to claim 3, which is characterized in that the ejection release piece (53) includes being capable of edge The short transverse (H) is supported on the ejection disengaging plate on the fixed pedestal (521) of the sampling gun actuator (52) up and down (531), which is located at the lower section of the main body (512), and the ejection in the short transverse (H) It is formed with multiple pipette tips interconnecting pieces on disengaging plate (531) and is inserted into hole (532), the pipette tips of each pipette tips interconnecting piece (514) connect Section (515) is connect respectively accordingly to penetrate through pipette tips interconnecting piece insertion hole (532) and be exposed to the ejection disengaging plate (531) The driving portion of side, the sampling gun actuator (52) is set as, under the retracted mode that the telescopic rod (511) is retracted, energy The ejection disengaging plate (531) is enough driven to squeeze each pipette tips interconnecting piece (514) along the off-direction of sampling hammerhead (200) On sampling hammerhead (200).

5. nucleic acid extraction mechanism according to claim 4, which is characterized in that the ejection release piece (53) includes supporting plate (533) and with described plate (533) and the ejection disengaging plate (531) connecting rod (534) for connecting are supported, it is described to support plate (533) it is located between the driving portion and the main body (512) of the sampling gun actuator (52), it is described to support plate (533) and institute Ejection disengaging plate (531) is stated by the connecting rod (534) along the short transverse (H) can be up and down arranged in the fixation On pedestal (521), the driving portion can abut under the retracted mode with the plate (533) of supporting, to push the top Go out release piece (53) to move along the off-direction, the connecting rod (534) passes through the reset that is set in the connecting rod (534) Spring (535) is resiliently supported on the fixed pedestal (521), and the both ends of the resetting spring (535) are supported with described respectively Plate (533) and the fixed pedestal (521) abut, and drive the ejection release piece (53) to return back to initially always for providing The elastic-restoring force of position.

6. nucleic acid extraction mechanism according to claim 4, which is characterized in that the sampling gun (51) is in transverse direction (X2) Be positioned apart from it is multiple, it is described to support plate (533) and the ejection disengaging plate (531) is prolonged along the horizontal direction (X2) respectively It stretches, the connecting rod (534) is multiple and along the horizontal direction (X2) interval, there are one the sides of the sampling gun (51) respectively Formula setting is supported described between plate (533) and the ejection disengaging plate (531), so that described support plate (533), the top Go out between connecting rod (534) described in disengaging plate (531) and adjacent each two and respectively constitutes sampling gun layout area, Ge Gesuo The main body (512) for stating sampling gun (51) is located in the sampling gun layout area.

7. nucleic acid extraction mechanism according to claim 2, which is characterized in that the sampling gun actuator (52) includes described Fixed pedestal (521), the sampling gun driving motor (522) being arranged on the fixed pedestal (521) drive electricity with the sampling gun The output shaft transmission of machine (522) is connected and is provided with the sampling gun transmission mechanism of the driving portion, and the sampling gun is driven The convert rotational motion of motor (522) is the linear motion of the driving portion.

8. nucleic acid extraction mechanism according to claim 7, which is characterized in that the sampling gun transmission mechanism is bolt and nut Transmission mechanism includes the output axis connection with the sampling gun driving motor (522) and is pivotally supported at the fixed base Seat (521) on the first screw rod (523) and first screw rod (523) can be movably arranged at along the short transverse (H) On the first nut (524), the driving portion is the driving baffle being set on the peripheral surface of first nut (524) (525), the holding tank (526) on the head for the telescopic rod (511) to be clamped is formed on the driving baffle (525).

9. the nucleic acid extraction mechanism according to any one of claim 1-8, which is characterized in that the magnetic bead transfer device (42) include magnetic bead transfer device fixed seat of the setting on the position of the close sampling hammerhead (200) of microscope carrier transport establishment (1) (421), the magnetic part actuator and magnetic part movable plate being arranged on the magnetic bead transfer device fixed seat (421) (422), which is arranged on the magnetic bead transfer device fixed seat (421), and is driven with the magnetic part Moving part connection with can be flexible along the direction closer or far from the sampling hammerhead (200), the magnetic part be magnet (423) and Mounted on the magnetic part movable plate (422) close to the side of the sampling gun (51).

10. nucleic acid extraction mechanism according to claim 9, which is characterized in that the magnetic part actuator includes magnetic part Driving motor (426) and magnetic part bolt and nut mechanism, magnetic part driving motor (426) setting is shifted in the magnetic bead to be filled It sets in fixed seat (421), magnetic part screw rod (427) and the magnetic part driving motor of magnetic part bolt and nut mechanism (426) output axis connection, magnetic part nut (428) and the magnetic part movable plate of magnetic part bolt and nut mechanism (422) it connects.

11. nucleic acid extraction mechanism according to claim 9, which is characterized in that formed on the magnetic part movable plate (422) The long guiding hole (424) for having the telescopic direction extension along the magnetic part movable plate (422), on the magnetic bead transfer device (42) It is provided with the positioning screw (425) for being inserted into and being installed in the long guiding hole (424), the magnetic part movable plate (422) magnetic bead is telescopically disposed in by the cooperation of the long guiding hole (424) and the positioning screw (425) to shift On device (42).

12. a kind of sample processing device, which is characterized in that the sample processing device includes microscope carrier transport establishment (1) and setting The nucleic acid extraction mechanism according to any one of claim 1-11 in the microscope carrier transport establishment (1).

13. a kind of method for extracting nucleic acid using the nucleic acid extraction mechanism according to any one of claim 1-11, special Sign is that the method for extracting nucleic acid includes：

Cleavage step (S21), the sampling hammerhead (200) draw sample and contain magnetic bead into the sample container (3) The sample is added in cell pyrolysis liquid so that obtaining adsorption after sample cracking has the bead complexes of nucleic acid, the sampling Pipette tips (200) draw the solution containing bead complexes, are stretched out close to the direction of the sampling hammerhead (200) in the magnetic part It is described under magnetic state, the sampling hammerhead (200) spues except the magnetic bead being adsorbed on the sampling hammerhead (200) inner wall is multiple Close the solution except object；

Washing step (S22), under the demagnetizing state that direction of the magnetic part far from the sampling hammerhead (200) is retracted, institute Sampling hammerhead (200) is stated to draw the cleaning solution in the sample container (3) and execute compressing action repeatedly, it is multiple to clean magnetic bead Close object, later described for magnetic state under, the sampling hammerhead (200), which spues, removes the solution containing bead complexes；

Elution step (S23), under the demagnetizing state, the sampling hammerhead (200) is drawn in the sample container (3) Eluent simultaneously executes compressing action repeatedly, so that the magnetic bead in bead complexes and nucleic acid separation, the sampling hammerhead (200) Draw the solution containing magnetic bead and nucleic acid；

Nucleic acid extraction step (S24), it is described for magnetic state under, the sampling hammerhead (200) will spit except the nucleic acid containing magnetic bead Go out and preserve to the sample container (3),

The method for extracting nucleic acid is in the sample container (3) in step-by-step movement traveling process, to be moved and be filled by the pipette tips The cooperation for setting the lifting action of (41) and the expanding-contracting action of the magnetic bead transfer device (42), is sequentially inserted into each sample The cleavage step (S21), the washing step (S22), the elution step are executed in the holding tank of container (3) (S23) and the nucleic acid extraction step (S24) and obtain nucleic acid.

14. method for extracting nucleic acid according to claim 13, which is characterized in that in the cleavage step (S21), described After sampling hammerhead (200) draws the solution containing bead complexes, further includes protein digestibility step (S25), gone described Under magnetic state, the sampling hammerhead (200) spues the solution containing bead complexes to the egg in the sample container (3) White matter digest enzyme solutions in and repeatedly execute compressing act, with the protein in digestion solution, later described for magnetic state under, The sampling hammerhead (200), which spues, removes the solution containing bead complexes, to complete the cleavage step (S21).