CN109207341A - sample processing device and sample processing method - Google Patents
sample processing device and sample processing method Download PDFInfo
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- CN109207341A CN109207341A CN201810847784.XA CN201810847784A CN109207341A CN 109207341 A CN109207341 A CN 109207341A CN 201810847784 A CN201810847784 A CN 201810847784A CN 109207341 A CN109207341 A CN 109207341A
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- microscope carrier
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- nucleic acid
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
Abstract
This disclosure relates to sample processing device and sample processing method, sample processing device includes microscope carrier transport establishment and multiple microscope carriers for being movably disposed in microscope carrier transport establishment, the working region arranged in microscope carrier transport establishment includes at least: the first working region, and first mechanical arm is for dispensing sample to each sample container of the microscope carrier in the first working region；It is provided with the second working region of nucleic acid extraction mechanism, nucleic acid extraction mechanism is configured to move along the direction close to or far from microscope carrier, for extracting nucleic acid contained in the sample in sample container and storing the nucleic acid of extraction to each sample container；Third working region, second mechanical arm detects nucleic acid for dispensing the nucleic acid of detection reagent and extraction into multiple deep-well plates, each microscope carrier by microscope carrier driving mechanism can along microscope carrier moving direction successively from the first working region move to second and third working region to execute relevant work.Thus sample processing device can improve sample process efficiency.
Description
Technical field
This disclosure relates to sample process technical field, and in particular, to a kind of sample processing device and sample processing method.
Background technique
In existing sample processing device, it is each on fixed microscope carrier to being loaded into generally use movable working arm
Sample container successively executes dispensing sample, extracts the processing operations such as nucleic acid, detection nucleic acid.And the sample process of this structure is set
Standby not only volume is big and there is a problem of that shared arrangement space is larger, but also since movable working arm is directed to the load of different location
The motion path of platform is also complex and causes to lead to the problem of sample process efficiency lower.
Summary of the invention
Purpose of this disclosure is to provide a kind of sample processing device that can be improved sample process efficiency and sample process sides
Method.
To achieve the goals above, according to one aspect of the disclosure, a kind of sample processing device is provided, the sample process
Equipment includes microscope carrier transport establishment and multiple microscope carriers for being movably disposed in the microscope carrier transport establishment, each microscope carrier
On be provided with multiple sample containers, useful multiple reagent solutions in processing sample are accommodated in each sample container, it is described
It is disposed with multiple working regions in microscope carrier transport establishment, first mechanical arm and the second machinery are provided in the microscope carrier transport establishment
Arm, the working region include at least: the first working region, and the first mechanical arm is positioned close to first working region
Position, and the first mechanical arm is set as, can be along the longitudinal direction for being parallel to microscope carrier moving direction and perpendicular to the load
The transverse direction of platform moving direction adjusts position, and can go up and down along short transverse, for dispensing sample to described first
In each sample container of the microscope carrier in working region；Second working region is provided with core in second working region
Sour extraction mechanism, which is configured to move along the direction close to or far from the microscope carrier, for extracting
Nucleic acid contained in sample in sample container, and the nucleic acid of extraction is stored to each sample container；Third work
Make region, the second mechanical arm is positioned close to the position of the third working region, and the second mechanical arm is set as, energy
It is enough to adjust position along the longitudinal direction and the transverse direction, and can be gone up and down along the short transverse, to be used for multiple
The nucleic acid of detection reagent and extraction is dispensed in deep-well plates respectively and detects nucleic acid, each microscope carrier passes through microscope carrier driving mechanism energy
It is enough successively to move to second working region and the third work from first working region along the microscope carrier moving direction
Make region, to execute relevant work.
According to another aspect of the present disclosure, a kind of sample process side using sample processing device as described above is provided
Method, the sample processing method include: first step, and microscope carrier is moved to first working region along microscope carrier moving direction, benefit
With dispensing sample in the first mechanical arm respectively each sample container on the microscope carrier；Second step, microscope carrier
It is moved to second working region from first working region along the microscope carrier moving direction, utilizes the nucleic acid extraction machine
Structure extracts nucleic acid contained in the sample in the sample container on microscope carrier, and the nucleic acid of extraction is stored to each sample
Container；Third step, microscope carrier are moved to the third working region from second working region, mechanical using described second
The nucleic acid stored in the sample container on the microscope carrier is dispensed in multiple deep-well plates that arm has detection reagent to dispensing and is detected
Nucleic acid, on the microscope carrier moving direction from first working region to the third working region, adjacent every two
Microscope carrier in microscope carrier positioned at front side is while executing any one step of the first step into third step, after being located at
The microscope carrier of side executes the previous step of any one step.
Pass through technical solution as described above, that is, use in the sample processing device of the disclosure in microscope carrier transport establishment
Move up the movable sample process mode of microscope carrier of dynamic load platform, and not in existing usually by mobile mechanical arm by the way of
To microscope carrier execute respective sample processing operation the fixed sample process mode of microscope carrier, therefore, the disclosure using movable load
The sample processing device of platform and the sample processing device phase under the existing middle stationary state using microscope carrier by mobile machine arm mode
Smaller than, equipment volume and multiple microscope carriers are performed simultaneously corresponding operation in corresponding working region, to significantly improve sample
Present treatment efficiency.
Other feature and advantage of the disclosure will the following detailed description will be given in the detailed implementation section.
Detailed description of the invention
Attached drawing is and to constitute part of specification for providing further understanding of the disclosure, with following tool
Body embodiment is used to explain the disclosure together, but does not constitute the limitation to the disclosure.In the accompanying drawings:
Fig. 1 is the perspective view one according to the sample processing device of disclosure specific embodiment；
Fig. 2 is the perspective view two according to the sample processing device of disclosure specific embodiment；
Fig. 3 is the main view according to the sample processing device of disclosure specific embodiment；
Fig. 4 is the side view according to the sample processing device of disclosure specific embodiment；
Fig. 5 is the cross-sectional view in Fig. 4 along line A-A cutting；
Fig. 6 is the cross-sectional view in Fig. 4 along line B-B cutting；
Fig. 7 is the top view according to the sample processing device of disclosure specific embodiment；
Fig. 8 is the cross-sectional view for rotating 180 ° in Fig. 7 after line C-C cutting, each in figure in order to be readily appreciated
Structure；
Fig. 9 is the cross-sectional view in Fig. 7 along line D-D cutting；
Figure 10 is each in upper layer microscope carrier shipping platform according to removing in the sample processing device of disclosure specific embodiment
The top view of a consumptive material, here, the consumptive material removed includes deep-well plates, sampling hammerhead, probe tube, dispensing pipette tips and detection reagent
Accommodate pipe；
Figure 11 is the cross-sectional view in Figure 10 along E-E line；
Figure 12 is the perspective view according to the nucleic acid extraction mechanism of disclosure specific embodiment；
Figure 13 is the pipette tips mobile device for being provided with pipette tips and ejecting releasing mechanism according to disclosure specific embodiment
Main view；
Figure 14 is the right view of Figure 13；
Figure 15 is the rearview of Figure 13；
Figure 16 is the main view that releasing mechanism is ejected according to the pipette tips of disclosure specific embodiment；
Figure 17 is the right view of Figure 16；
Figure 18 is the cutaway view Amplified image in Figure 17 along F-F line cutting；
Figure 19 is that the perspective view that sampling gun is removed in releasing mechanism is ejected according to the pipette tips of disclosure specific embodiment；
Figure 20 is the perspective view one according to the magnetic bead transfer device of disclosure specific embodiment；
Figure 21 is the perspective view two according to the magnetic bead transfer device of disclosure specific embodiment；
Figure 22 is the perspective view according to the cooperation assembly of the microscope carrier and microscope carrier transport establishment of disclosure specific embodiment；
Figure 23 is to be supported according to omission in the microscope carrier of disclosure specific embodiment and the cooperation assembly of microscope carrier transport establishment
The perspective view of frame etc.；
Figure 24 is the side view of Figure 23；
Figure 25 is to reduce figure along the section view of G-G line cutting in Figure 24；
Figure 26 is the cutaway view Amplified image in Figure 25 along I-I line cutting；
Figure 27 is the cutaway view Amplified image in Figure 25 along J-J cutting；
Figure 28 is the perspective view according to the microscope carrier driven in translation structure of disclosure specific embodiment；
Figure 29 is the main view of Figure 28；
Figure 30 is the cutaway view Amplified image in Figure 29 along K-K line cutting；
Figure 31 is the perspective view according to the movable trolley of disclosure specific embodiment；
Figure 32 is the perspective view one according to the microscope carrier of disclosure specific embodiment；
Figure 33 is the perspective view two according to the microscope carrier of disclosure specific embodiment；
Figure 34 is the top view according to the microscope carrier of disclosure specific embodiment；
Figure 35 is the cutaway view Amplified image in Figure 34 along L-L line cutting；
Figure 36 is the bottom view according to the microscope carrier of disclosure specific embodiment；
Figure 37 is the structure chart of the first pulley and first pulley mounting base in Figure 36；
Figure 38 is the cross-sectional view in Figure 37 along S-S line cutting；
Figure 39 is the perspective view one according to fixing seat in the microscope carrier of disclosure specific embodiment；
Figure 40 is the perspective view two according to fixing seat in the microscope carrier of disclosure specific embodiment；
Figure 41 is the perspective view one according to sliding seat in the microscope carrier of disclosure specific embodiment；
Figure 42 is the perspective view two according to sliding seat in the microscope carrier of disclosure specific embodiment；
Figure 43 is the step flow diagram according to the method for extracting nucleic acid of disclosure specific embodiment；
Figure 44 is the step flow diagram according to the sample processing method of disclosure specific embodiment.
Description of symbols
1- microscope carrier transport establishment, 11- microscope carrier shipping platform, the upper layer 111- microscope carrier shipping platform, the transport of 112- lower layer microscope carrier
Platform, 12- microscope carrier driven in translation structure, the first driving motor of 121-, 122- activity trolley, the first transmission belt of 123-, 124-
One driving wheel, the first driven wheel of 125-, 126- trolley guide rail, 127- second pulley component, 128-second pulley mounting bases, 13-
Microscope carrier goes up and down driving structure, and 131- goes up and down fixed pedestal, 132- lift cylinders, and 133- goes up and down fixed pedestal guide rail, and 14- microscope carrier picks and places
Opening, the second positioning seat of 15-, the first magnet plunger of 151-, 16- groove profile photoelectrical position sensor, 17- microscope carrier guide rail, 171- microscope carrier
Mating groove, 172- microscope carrier retention bead, 18- microscope carrier return driving structure, the second driving motor of 181-, the second transmission belt of 182-,
The second driving wheel of 183-, the second driven wheel of 184-, 185- transmission shaft, 19- braced frame,
2- microscope carrier, 21- locking structure, the first positioning seat of 22-, 221- locating slot, 222- block, 23- first pulley component,
231- first pulley mounting base, the fixed pulley of 232- first, the first movable pulley of 233-, 2331- wheel shaft, the first activity of 2332-
Pulley bearings, 234- first pulley install block hole, 235- screw thread, 236- fastening nut, 237- microscope carrier guide rail mating groove, 238-
Fastening nut mounting groove, 24- fixing seat, 241- fixing seat bottom plate, 242- fixing seat side wall, 243- sliding seat accommodating chamber, 244-
Anti-detachment protrusion, 25- sliding seat, 251- container holding tank, 252- mistake proofing assembling structure, 253- sliding seat pedestal, 254- sliding seat
Top plate, 26- sliding seat guidance set, 261- guide groove, 262- guide protrusions, 27- magnetic absorption member, 28- electromagnet latch, 29-
Internal standard holding tank,
3- sample container,
4- nucleic acid extraction mechanism, 41- pipette tips mobile device, 411- pedestal, 412- releasing mechanism driving motor, 413- are detached from
Mechanism screw-and-nut mechanism, 414- releasing mechanism guide frame, 415- fixed pedestal position sensor, the transfer of 42- magnetic bead
Device, 421- magnetic bead transfer device fixing seat, the mobile panel guide of 4211-, 422- magnetic part movable plate, 423- magnet, 424- are led
To long hole, 425- positioning screw, 426- magnetic part driving motor, 427- magnetic part screw rod, 428- magnetic part nut, 429- magnetism
Part movable plate position sensor,
5- pipette tips eject releasing mechanism, 51- sampling gun, 511- telescopic rod, 512- main body, 513- telescopic rod channel, 514-
Pipette tips interconnecting piece, 515- pipette tips linkage section, 516- seal section, 517- locking protrusion, 52- sampling gun actuator, the fixed base of 521-
Seat, 5211- connecting rod mounting hole, 5212- axle sleeve, 5211- assembling stand, 522- sampling gun driving motor, the first screw rod of 523-,
524 first nuts, 525- driving baffle, 526- holding tank, 527- vertical plate, 528- upper water plate, 5281- guide rod,
5282- guideway, 5283- drive baffle position sensor, 529- lower horizontal plate, 5291- main body holding tank, 53- ejection
Release piece, 531- eject disengaging plate, and 532- pipette tips interconnecting piece is inserted into hole, and 533- supports plate, 5331- telescopic rod holding tank, 534-
Connecting rod, 535- reset spring,
6- first mechanical arm, 7- second mechanical arm, 8- stretching structure, the second magnet plunger of 81-, 82- electromagnet,
100- deep-well plates, 200- sampling hammerhead, 300- probe tube, 400- dispense pipette tips, and 500- detection reagent accommodates pipe,
The first working region A-, the second working region B-, C- third working region, the initial storage area of D- microscope carrier, E-
One consumptive material storage area, E1- sampling hammerhead storage area, E2- probe tube storage area, the second consumptive material of F- storage area, F1- points
Pipette tips storage area, F2- deep-well plates storage area are infused, F3- detection reagent accommodates pipe storage area,
Z1- microscope carrier moving direction, Z2- nucleic acid extraction direction, X1- longitudinal direction, X2- transverse direction, H- short transverse, P-
First telescopic direction,
S1- first step, S11- internal standard dispense step, and S12- sample dispenses step, S2- second step, S21- cracking step
Suddenly, S22- washing step, S23- elution step, S24- nucleic acid extraction step, S25- protein digestibility step, S3- third step,
S31- detection reagent dispenses step, and S32- nucleic acid dispenses step, S4- microscope carrier return step.
Specific embodiment
It is described in detail below in conjunction with specific embodiment of the attached drawing to the disclosure.It should be understood that this place is retouched
The specific embodiment stated is only used for describing and explaining the disclosure, is not limited to the disclosure.
In the disclosure, in the absence of explanation to the contrary, the noun of locality used such as " upper and lower, left and right " typically refers to
" upper and lower, left and right " of corresponding component under use state, " inside and outside " refer to " inside and outside " for corresponding component outer profile,
This, the mode of microscope carrier 2 is driven to clearly illustrate microscope carrier driven in translation structure 12 in the second working region B, mobile with microscope carrier
On the basis of the Z1 of direction, it is defined as rear side positioned at the upstream side microscope carrier moving direction Z1, is located at the downstream side microscope carrier moving direction Z1 and defines
For front side, the first working region A and third working region C are located at rear side and the front side of the second working region B.In addition, this
" the microscope carrier moving direction " referred in open typically refers to substantially successively move from the first working region A on the basis of each microscope carrier
It to the second working region B, third working region C and is finally repositioned to the direction of the initial storage area D of microscope carrier, as hollow closes
Circumferential direction, can refer to the direction Z1 as shown in figure 11 herein, and " the nucleic acid extraction direction " referred in the disclosure usually exists
The direction of motion of microscope carrier is directed to during second working region B, that is, nucleic acid extraction, nucleic acid extraction direction can be and above-mentioned load
Platform moving direction it is identical or can in contrast to above-mentioned microscope carrier moving direction, here, in the preferred embodiment of the disclosure with
Nucleic acid extraction has carried out in detail sample processing device and sample processing method in the direction opposite the example of microscope carrier moving direction
Ground explanation, i.e., can refer to the direction Z2 as shown in Fig. 5 and Figure 11 in the nucleic acid extraction direction in following preferred embodiments.
Firstly, present disclose provides a kind of sample processing device and sample processing method, the sample processing device of the disclosure
It can be used for detecting the nucleic acid for representing life entity hereditary feature basic unit with method, for example, the sample processing device of the disclosure
It can be applied to the detection and diagnosis of the infectious diseases such as hepatitis, AIDS, influenza, hand-foot-and-mouth disease with sample processing method, can also use
In detecting and diagnosing Other diseases, can be automated by the sample processing device and sample processing method of the disclosure such as flowering structure
Execute sample automatic filling, nucleic acid extraction and detection nucleic acid equal samples and handle operation, realize sample process process it is complete from
Dynamicization operation.
Here, as shown in Figure 1 to 11, the sample processing device of the disclosure may include microscope carrier transport establishment 1 and removable
Multiple microscope carriers 2 in microscope carrier transport establishment 1 are set dynamicly, multiple sample containers 3, each sample are provided on each microscope carrier 2
Useful multiple reagent solutions in processing sample are accommodated in this container 3, are disposed with multiple workspaces in microscope carrier transport establishment 1
Domain is provided with first mechanical arm 6 and second mechanical arm 7 in microscope carrier transport establishment 1, and the working region includes at least: the first work
Make region A, first mechanical arm 6 is positioned close to the position of the first working region A, and the first mechanical arm 6 is set as, Neng Gouyan
It is parallel to the longitudinal direction X1 of microscope carrier moving direction Z1 and adjusts position perpendicular to the transverse direction X2 of microscope carrier moving direction Z1, and
It can be gone up and down along height direction H, for dispensing sample to each sample container 3 of the microscope carrier 2 in the first working region A
It is interior；It is provided with nucleic acid extraction mechanism 4 in second working region B, second working region B, which is arranged to
It reaches and is moved along the direction close to or far from microscope carrier 2, for extracting nucleic acid contained in the sample in sample container 3, and general
The nucleic acid of extraction is stored to each sample container 3；Third working region C, second mechanical arm 7 are positioned close to third workspace
The position of domain C, and the second mechanical arm 7 is set as, and can adjust position along the longitudinal direction X1 and transverse direction X2,
And can be gone up and down along the height direction H, with the nucleic acid for dispensing detection reagent and extraction respectively into multiple deep-well plates 100
And nucleic acid is detected, each microscope carrier 2 can successively be transported from the first working region A by microscope carrier driving mechanism along microscope carrier moving direction Z1
It moves to the second working region B and third working region C, to execute relevant work.That is, being used in the sample processing device of the disclosure
The movable sample process mode of microscope carrier that moves up dynamic load platform 2 in microscope carrier transport establishment 1, and not use usually logical in existing
The mode for crossing mobile mechanical arm executes the fixed sample process mode of microscope carrier of respective sample processing operation to microscope carrier, therefore, this
The disclosed sample processing device using movable microscope carrier and existing middle use pass through mobile machine arm side under microscope carrier stationary state
The sample processing device of formula is compared, and equipment volume is small and multiple microscope carriers 2 are performed simultaneously accordingly in corresponding working region
Operation, to significantly improve sample process efficiency.
Specifically, sample processing device as described above has the following course of work.That is, passing through microscope carrier driving mechanism
Driving, microscope carrier 2 is moved to the first working region A along microscope carrier moving direction Z1, using first mechanical arm 6 respectively on microscope carrier 2
Sample is dispensed in each sample container 3.Dispensing has the microscope carrier 2 of sample from the first working region A in sample container 3 later
It is moved to the second working region B along microscope carrier moving direction Z1, extracts the sample container 3 on microscope carrier 2 using nucleic acid extraction mechanism 4
Nucleic acid contained in interior sample, and the nucleic acid of extraction is stored to each sample container 3.Here, can using paramagnetic particle method,
The modes such as centrifugal column method execute nucleic acid extraction operation, and nucleic acid extraction mechanism 4 can be extracted according to actual nucleic acid and used
Mode carrys out reasonable design into a variety of arrangements, such as using paramagnetic particle method nucleic acid extraction mode, nucleic acid extraction
Mechanism 4 can correspondingly include magnetic force generation device and the sample for executing the sample transfer tasks for sample container 3
Transfer device etc..After completing nucleic acid extraction, the microscope carrier 2 of nucleic acid is placed with from the second working region B in 3 memory of sample container
It is moved to third working region C, there is dispensing microscope carrier 2 in multiple deep-well plates 100 of detection reagent to dispensing using second mechanical arm 7
On sample container 3 in store nucleic acid and detect nucleic acid.Multiple microscope carriers 2 can be in corresponding working region as a result,
It is inside performed simultaneously corresponding work, thus, it is possible to significantly improve the sample process operating efficiency entirely recycled.
The movement of microscope carrier 2 in sample processing device as described above can be by carrying the setting of microscope carrier driving mechanism
It, can also be using the cooperation reality by microscope carrier driving mechanism and microscope carrier 2 on platform 2 in a manner of the active drive that realization voluntarily controls
The passive matrix mode now passively controlled, the disclosure are not particularly limited the structure of microscope carrier driving mechanism, as long as can be real
The now function that driving microscope carrier 2 moves in microscope carrier transport establishment 1 along microscope carrier moving direction Z1.In the disclosure, for letter
Change stage structure in order to movement, and in order to enable the drive control mode of microscope carrier 2 is more simplified, sample processing device is to adopt
The passive matrix mode for using microscope carrier driving mechanism and microscope carrier to cooperate is described in aftermentioned as specific embodiment.
With reference first to Figure 12 to Figure 21 to can be suitable for the nucleic acid extraction mechanism sample processing device as described above
Structure, feature and function and effect be described.
As shown in Figure 12 to Figure 15, the nucleic acid extraction mechanism of the disclosure utilizes the sample with multiple sample holding tanks to accommodate
Device 3 extracts nucleic acid, and the nucleic acid extraction mechanism 4 is arranged in microscope carrier transport establishment 1, and includes: pipette tips mobile device 41, pipette tips
Mobile device 41 can be arranged in up and down in microscope carrier transport establishment 1 along height direction H, rifle is separatably arranged in for driving
Sampling hammerhead 200 on head moving device 41 enables to sampling hammerhead 200 to adsorb sample along height direction H shift position
The liquid in liquid or discharge sampling hammerhead 200 in container 3；Magnetic bead transfer device 42, the magnetic bead transfer device 42 include
Magnetic part, magnetic part can be telescopically arranged in microscope carrier transport establishment 1 along the direction close to or far from sampling hammerhead 200, with
So that magnetic part, which has, acts on sampling hammerhead 200 for magnetic state and removing to the magnetic bead offer magnetic force in sampling hammerhead 200
The demagnetizing state of the magnetic force of interior magnetic bead.As described above, the nucleic acid extraction mechanism of the disclosure extracts nucleic acid using paramagnetic particle method, specifically
Ground, during extracting nucleic acid, the nucleic acid for be released after cell cracking to sample first is mixed with magnetic bead, is formed
Nucleic acid-bead complexes, at this time can be by the decline of pipette tips mobile device 41 so that being loaded in pipette tips mobile device 41
Sampling hammerhead 200 adsorbs the bead complexes, passes through the lifting action of pipette tips mobile device 41 and magnetic bead transfer dress later
42 expanding-contracting actions towards the direction close to or far from sampling hammerhead 200 are set, which is transferred to sample respectively and is held
It receives in each sample holding tank of device 3 and executes and the operations such as correspondingly wash, elute.Specifically, in multiple sample holding tanks
Reaction reagent (for example, cell pyrolysis liquid, cleaning solution, eluent etc.) between transfer nucleic acid-bead complexes when, nucleic acid-magnetic
Pearl compound is placed in always in sampling hammerhead 200, and nucleic acid-magnetic bead is compound in the case where the offer of magnetic bead transfer device 42 is for magnetic state
Object is adsorbed on the inner wall of sampling hammerhead 200, in this case by sampling hammerhead 200 waste liquid (such as complete cracking cracking
Waste liquid, the scrub raffinate for completing washing, the elution waste liquid for completing elution etc.) it is expelled to the corresponding sample appearance of sample container 3
It receives in slot, sampling hammerhead 200 holds next sample that internal nucleic acid-bead complexes are expelled to sample container 3 later
It receives in slot.And in existing sample extraction equipment, generally use mechanical arm moves in microscope carrier transport establishment, and microscope carrier and sample
The container nucleic acid extraction mode fixed relative to microscope carrier transport establishment, in the case, during nucleic acid extraction, pass through by
Bead complexes are placed in always in the state of sample container, and waste liquid and injection in sample container are drawn by sampling hammerhead
The mode of new reaction reagent executes nucleic acid extraction operation.Specifically, it is accommodated in existing in nucleic acid-bead complexes and sample
After a certain reaction reagent in device is sufficiently mixed, magnetic force generation device provides magnetic force to sample container, so that nucleic acid-magnetic bead is multiple
It closes object to be adsorbed on sample container inner wall, at this point, mechanical arm driving sampling hammerhead draws the reaction reagent in sample container
Waste liquid and exclude, while the sampling hammerhead that discarded use is crossed, later mechanical arm reload new sampling hammerhead by another
Reaction reagent is injected into sample container and is mixed with nucleic acid-bead complexes, makees to successively execute nucleic acid extraction
Industry.This sample processing device in existing is during nucleic acid extraction, since a reaction reagent is corresponding using a sampling
Pipette tips lead to the serious waste of sampling hammerhead, and hold each sample often through the mode of mobile magnetic force generation device
Device of receiving is executed for magnetic or degaussing operation, or respectively accordingly arrangement magnetic force generates dress in the position close to each sample container
It sets, so that the nucleic acid extraction operation of sample processing device is increasingly complex, and there is the problem of manufacture and processing cost valuableness.
And for the nucleic acid extraction mechanism and sample processing device of the disclosure, nucleic acid extraction mechanism 4 in microscope carrier transport establishment 1 by setting
Set pipette tips mobile device 41 and magnetic bead transfer device 42, wherein the magnetic part of magnetic bead transfer device 42 is in microscope carrier transport establishment 1
It is set as, can be flexible along the direction close to or far from the sampling hammerhead 200, thus during nucleic acid extraction, in sample
Receiving is useful in the state of each reaction reagent for extracting nucleic acid in each sample holding tank of container 3, in corresponding sample
Bead complexes containing sample and magnetic bead after reacting in this holding tank follow sampling hammerhead 200 mobile always, therefore every
The nucleic acid extraction of a sample only needs to can be realized as by a sampling hammerhead 200, thereby saves sampling hammerhead consumptive material, drop
Low nucleic acid extraction cost, and due to executing nucleic acid in the state of accommodating each reaction reagent in each sample holding tank
Operation is extracted, it is molten after thus reacting in the sampling hammerhead 200 correspondence sample holding tanks that need to shift sample container 3
Liquid, without being executed again such as the existing middle movement for injecting corresponding reaction reagent into each holding tank, so that whole mention
High nucleic acid extraction operating efficiency.In addition, during nucleic acid extraction, due to 41 drive sampling hammerheads of pipette tips mobile device
200 along height direction H shift position, therefore magnetic bead transfer device 42 can be made relatively-stationary in microscope carrier transport establishment 1
Under state, by the expanding-contracting action of the magnetic part of magnetic bead transfer device 42 be convenient for providing to nucleic acid-bead complexes magnetic force or
Magnetic force is removed, so that nucleic acid extraction simple operation, reduces the manufacture of nucleic acid extraction mechanism and sample processing device
And processing cost.
Here, the arrangement of various reasonable, example can be used for pipette tips mobile device 41 and magnetic bead transfer device 42
Such as, optionally, pipette tips mobile device 41 includes the pedestal 411 being arranged in microscope carrier transport establishment 1 and can be along the short transverse
The ejection releasing mechanism 5 of the pipette tips on pedestal 411 is arranged in H up and down.Here, can be suitable for the rifle pipette tips mobile device 41
The crown goes out releasing mechanism 5 and is configured to such as flowering structure, that is, as shown in Figure 16 to Figure 19, pipette tips ejection releasing mechanism 5 be can wrap
Sampling gun 51, sampling gun actuator 52 and the ejection release piece 53 for installing sampling hammerhead 200 are included, sampling gun actuator 52
Driving portion connect with the telescopic rod 511 of sampling gun 51 and for driving telescopic rod 511 flexible, so that sampling gun 51 is sampling gun
First 200 provide the pressure of the suction of imbibition or drain, and ejection release piece 53 is mounted on sampling gun actuator 52 and enables to
Sampling hammerhead 200 is separated with sampling gun 51.As described above, in the case where sampling hammerhead 200 is loaded into the use state of sampling gun 51,
By the driving of the driving portion of sampling gun actuator 52, sampling gun 51 carries out expanding-contracting action and makes 200 liquid draw of sampling hammerhead
Body or discharge liquid, pipette tips 200 to be sampled can make sampling gun by ejecting release piece 53 after completing nucleic acid extraction operation
First 200 easily fall out from sampling gun 51, improve the efficiency of loading and unloading of sampling hammerhead 200, thus, it is possible to nucleic acid is effectively ensured to mention
It is taken as the reliability of industry.
In addition, here, ejection release piece 53 can be set to the structure of various reasonable, as long as can be by sampling hammerhead
200 apply the active force towards off-direction, realize the function that sampling hammerhead 200 is detached from from sampling gun 51.For example, ejection
Release piece 53 can be set into mobilizable clamping limb, clamp sampling hammerhead 200 by the clamping limb and by sampling hammerhead
200 are detached from from sampling gun 51, and for another example, optionally, as shown in fig. 16 and 18, sampling gun 51 includes main body 512 and multiple flexible
Bar 511, main body 512 are arranged on the fixed pedestal 521 of sampling gun actuator 52, and have the both ends being inserted into for telescopic rod 511
Multiple telescopic rod channels 513 of opening, the top of each telescopic rod 511 are connect with the driving portion of sampling gun actuator 52, and each
A telescopic rod 511 can telescopically be inserted into corresponding telescopic rod channel by the driving of driving portion along height direction H
In 513, the bottom corresponding to each telescopic rod channel 513 of main body 512 be respectively structured as with corresponding sampling hammerhead 200
The pipette tips interconnecting piece 514 of connection, ejection release piece 53 is configured to move relative to each pipette tips interconnecting piece 514, with can
So that each sampling hammerhead 200 is separated with corresponding pipette tips interconnecting piece 514.Wherein, it is formed in main body 512 multiple flexible
Bar channel 513, here, each telescopic rod channel 513 is with the close extension end of corresponding telescopic rod 511 (in height under use state
Spend direction H on top) part between for sealing cooperation, it can realize telescopic rod channel by way of arranging sealing ring
Sealing cooperation between 513 and telescopic rod 511, with when telescopic rod 511 does extending action in telescopic rod channel 513, due to
Area of low pressure is formed in telescopic rod channel 513, liquid can pass through the liquid sucting port of sampling hammerhead 200 under external atmosphere pressure effect
It enters in sampling hammerhead 200.The working principle of this sampling gun 51 is similar with the working principle of syringe.In addition, pipette tips connect
Socket part 514 can be formed as the arrangement of various reasonable, for example, to may be integrally formed at main body 512 right for pipette tips interconnecting piece 514
It should be in the bottom in telescopic rod channel 513, alternatively, pipette tips interconnecting piece 514 can also for the ease of the structure of machine-shaping sampling gun 51
To form the bottom for being independent component and being connected to main body 512 corresponding to telescopic rod channel 513.Pass through structure as described above
Pipette tips eject releasing mechanism 5, it can by multiple sampling hammerheads 200 and with the sample container 3 of multiple groups sample holding tank
Cooperation, operations such as synchronous nucleic acid extraction for executing multiple samples, and make multiple sampling hammerheads by ejecting release piece 53
200 disposably fall off from each pipette tips interconnecting piece 514 simultaneously, and the dismounting for thus effectively saving sampling hammerhead 200 is taken
Between, nucleic acid extraction efficiency equal samples treatment effeciency can be significantly improved.
Optionally, as shown in Figure 17 and Figure 19, ejection release piece 53 includes that can support up and down along the height direction H
Ejection disengaging plate 531 on the fixed pedestal 521 of sampling gun actuator 52, the ejection disengaging plate 531 is in the height direction H
The upper lower section positioned at main body 512, and eject and be formed with multiple pipette tips interconnecting piece insertions hole 532 on disengaging plate 531, each pipette tips connect
Respectively accordingly perforation pipette tips interconnecting piece is inserted into hole 532 and is exposed to ejection disengaging plate 531 the pipette tips linkage section 515 of socket part 514
Side, the driving portion of sampling gun actuator 52 is set as, and under the retracted mode that telescopic rod 511 retracts, can drive ejection
Disengaging plate 531 squeezes the sampling hammerhead 200 on each pipette tips interconnecting piece 514 along the off-direction of sampling hammerhead 200.That is, in order to
Sampling hammerhead 200 more efficiently is unloaded in the state of guaranteeing that sampling hammerhead 200 empties liquid, ejects disengaging plate 531
Make motion path smallizationer in the position of neighbouring sampling hammerhead 200 and act rapidly on sampling hammerhead 200, specifically,
Sampling hammerhead 200 is mounted on the part for being exposed to the side of ejection disengaging plate 531, in the driving portion of sampling gun actuator 52
In the state that driving telescopic rod 511 is fully retracted, ejection disengaging plate 531 can further be driven to move along off-direction, so that
Ejection disengaging plate 531 and each sampling hammerhead 200 are in contact and push each sampling hammerhead 200 along off-direction, thus respectively
A sampling hammerhead 200 can fall off from each pipette tips interconnecting piece 514 simultaneously.Pipette tips are enabled to by structure as described above
The integral arrangement of ejection releasing mechanism 5 is more rationalized.In addition, since the telescopic rod for driving sampling gun 51 is utilized
The driving source that 511 flexible sampling gun actuators 52 are moved as driving ejection disengaging plate 531, there is no need to individually arrangement is used again
In the independent driving source that driving ejection disengaging plate 531 moves, saves manufacturing cost and can satisfy so that pipette tips ejection is detached from
The requirement of overall structure simplerization of mechanism 5.
Here, in order to guarantee that the driving portion of sampling gun actuator 52 steadily and reliably drives ejection disengaging plate 531 to move
And be in contact with each sampling hammerhead 200, and make pipette tips ejection releasing mechanism 5 each structure arrangement it is more compact and
Rationally, optionally, as shown in figure 19, the ejection release piece 53 includes supporting plate 533 and being detached from supporting plate 533 and ejecting
The connecting rod 534 that plate 531 connects, supports plate 533 and is located between the driving portion and main body 512 of sampling gun actuator 52, support plate
533 can be arranged in up and down on fixed pedestal 521 with ejection disengaging plate 531 by connecting rod 534 along the height direction H,
Driving portion can abut under the retracted mode with plate 533 is supported, to push ejection release piece 53 to move along the off-direction
Dynamic, connecting rod 534 is resiliently supported on fixed pedestal 521 by the reset spring 535 being set in the connecting rod 534, is resetted
The both ends of spring 535 respectively with support plate 533 and fixed pedestal 521 abuts, to drive ejection release piece 53 always for providing
Return back to the elastic-restoring force of initial position.Wherein, connecting rod mounting hole 5211 can be set on fixed pedestal 521, connected
Bar 534 is cooperated in connecting rod mounting hole 5211 by structures such as axle sleeves 5212, in 534 lifting process bottom bracket axle of connecting rod
The structures such as 5212 play the role of protecting connecting rod 534 and effectivelying prevent connecting rod 534 that movement abrasion occurs.Wherein, in order to just
In all parts of assembly pipette tips ejection releasing mechanism 5, and the structure for ejecting release piece 53 and sampling gun 51 is arranged more
Adduction is physical and chemical, optionally, supports and could be formed with the telescopic rod holding tank 5331 passed through for each telescopic rod 511 on plate 533,
This to eject under the original state that ejection release piece 53 is in idle by the elastic anchorage force that reset spring 535 provides
Release piece 53 is supported integrally on fixed pedestal 521, in the case where ejection release piece 53 is in running order, sampling gun actuator 52
It driving portion and supports plate 533 and is in contact and makes ejection de- by guiding role of the connecting rod 534 on fixed pedestal 521
Off member 53 is whole mobile towards off-direction (i.e. in height direction H downwards), in the process, ejection disengaging plate 531 and each
A sampling hammerhead 200 is in contact and each sampling hammerhead 200 is pushed to connect it from the pipette tips of corresponding sampling gun 51
Portion 514 falls off.In addition, being removed in sampling gun actuator 52 to supporting plate 533 after the unloading for completing sampling hammerhead 200
When active force, release piece 53 is ejected entirely through the elastic-restoring force of reset spring 535 and is returned to idle initial position.By
This, by structure as described above can handling operation that is accurate and fast implementing sampling hammerhead 200, nucleic acid extraction is effectively ensured
Operation operates normally.But it's not limited to that for the disclosure, and the specific structure for ejecting release piece 53 can be according to sample process
The actual arrangement structure of equipment carrys out reasonable design, for example, can be used alone another for the driving method for ejecting release piece 53
Outer driving source and do not generated with sampling gun actuator 52 and linkedly directly drive the movement of ejection release piece 53 and make ejection de-
Off member 53 and sampling hammerhead 200 cooperate and realize the unloading of sampling hammerhead 200 from pipette tips interconnecting piece 514.
Here, in order to further increase nucleic acid extraction equal samples treatment effeciency, optionally, as shown in figure 12, sampling gun 51
X2 has been positioned apart from multiple in transverse direction, supports the X2 extension in transverse direction respectively of plate 533 and ejection disengaging plate 531, even
Extension bar 534 is that mode that is multiple and being separated with a sampling gun 51 between X2 in transverse direction respectively is arranged in and supports plate 533 and ejection
Between disengaging plate 531, divide between plate 533, ejection disengaging plate 531 and every adjacent every two connecting rod 534 so that supporting
Not Gou Cheng sampling gun layout area, the main body 512 of each sampling gun 51 is located in the sampling gun layout area.Wherein, each
It can be arranged with reset spring 535 as described above in connecting rod 534, enhance and the whole resilient support of ejection release piece 53 is imitated
Fruit to support plate 533 and ejection disengaging plate 531 is firmly connected as overall structure, improves ejection by multiple connecting rods 534
The whole bonding strength of release piece 53 and rigidity, effectively avoid occuring bending and deformation during pushing multiple sampling hammerheads
Phenomenon.In addition, in the specific embodiment of the disclosure, for example, sampling gun 51 can be positioned apart from four with X2 in transverse direction
It is a, and there are four telescopic rod channel 513 and four telescopic rods 511 for setting in each sampling gun 51, it can be by as described above
Four sampling guns 51 and the nucleic acid extraction equal samples processing operation that disposably can synchronously execute 16 samples, and pass through
The disposable synchronously execution unloading of 16 sampling hammerheads 200 is enabled to make using plate 533 and ejection disengaging plate 531 is supported
Industry, so that ejection release piece 53 is more simple for the unloading operation of sampling hammerhead 200 and is easily achieved.
Here, optionally, as shown in figure 18, each pipette tips interconnecting piece 514 includes the bottom end opening with telescopic rod channel 513
The seal section 516 for sealing cooperation, the pipette tips linkage section 515 to connect with the seal section 516, being interval in for pipette tips linkage section 515 are close
The locking protrusion 517 being formed on the position of section 516 for being engaged by clamping with sampling hammerhead 200 is sealed, ejection disengaging plate 531 is located at
Between seal section 516 and locking protrusion 517, locking protrusion 517 is arranged to be inserted into hole 532 across pipette tips interconnecting piece.Here,
By the main body 512 of pipette tips interconnecting piece 514 and sampling gun 51 be arranged to separate structure be for the ease of process and assemble pipette tips ejection
The all parts of releasing mechanism 5, and also have the effect of convenient for follow-up maintenance and reduce maintenance cost.In addition, that will sample
When pipette tips 200 are loaded into pipette tips linkage section 515, due to the locking protrusion 517 and the hair of sampling hammerhead 200 on pipette tips linkage section 515
It is raw to be engaged by clamping, to guarantee that sampling hammerhead 200 holds normal imbibition and draining function, in nucleic acid extraction equal samples treatment process
In avoid there is a phenomenon where sampling hammerhead 200 from pipette tips linkage section 515 be detached from.
Optionally, as shown in figure 19, sampling gun actuator 52 includes fixed pedestal 521, is arranged on the fixed pedestal 521
Sampling gun driving motor 522, with the transmission of the output shaft of sampling gun driving motor 522 be connected and be provided with the sampling gun of driving portion
Transmission mechanism converts the rotary motion of sampling gun driving motor 522 to the linear motion of driving portion.Wherein, fixed pedestal
521 can be set on the pedestal 411 of pipette tips mobile device 41, and sampling gun transmission mechanism can use the arrangement knot of various reasonable
Structure, as long as can be realized the function of converting the rotary motion of sampling gun driving motor 522 to the linear motion of driving portion,
For example, sampling gun transmission mechanism can be rack pinion matching mechanism, Worm Wheel System matching mechanism, worm drive machine
Structure etc..Here, optionally, sampling gun transmission mechanism is screw-and-nut mechanism, including defeated with sampling gun driving motor 522
It axis connection and the first screw rod 523 being pivotally supported on fixed pedestal 521 and can movably be set along height direction H out
The first nut 524 on the first screw rod 523 is set, driving portion is the driving baffle being set on the outer peripheral surface of the first nut 524
525, the holding tank 526 on the head for clamping telescopic rod 511 is formed on the driving baffle 525.Here, the first screw rod 523
It can be pivotally supported at by bearing on fixed pedestal 521, here, for the ease of the first nut of connection 524 and driving gear
Plate 525, the first nut 524 can be formed as T-nut, and driving baffle 525 is set on the small head end of the T-nut and passes through
Bolt and the stub end of T-nut are fixed together, and the connection of driving baffle 525 and the first nut 524 has thus been effectively ensured
Reliability.In addition, X2 extends and can be formed driving baffle 525 in transverse direction in the case where sampling gun is provided with multiple
For with the development length roughly the same with the development length for supporting plate 533, to enable to driving baffle 525 to supporting plate
533 apply uniform power, are effectively ensured to support plate 533, connecting rod 534 and eject 531 entirety of disengaging plate and put down along height direction H
Quietly move.In addition, for the ease of the telescopic rod 511 of sampling gun 51 to be assembled on driving baffle 525, optionally, holding tank
526 are formed to have the C-channel of opening, and the head of telescopic rod 511 circumferentially could be formed with the inside for snapping fit onto C-channel
Clamping engagement groove on wall, thus telescopic rod 511 is by snapping fit onto C-channel clamping engagement slot from the opening of C-channel, by
While this guarantees telescopic rod 511 and drives the assembly reliability between baffle 525, realize to telescopic rod 511 relative to drive
Dynamic limit of the baffle 525 in height direction H.Have by using screw-and-nut mechanism as described above and matches occlusal wear
It is small, transmission efficiency is high, stable drive, service life are long and advantage with high accuracy.
In order to meet the lightweight of pipette tips ejection releasing mechanism 5 and save the demand of manufacturing cost, optionally, fixed base
Seat 521 is including vertical plate 527 and along height direction H arranged for interval in the upper water plate 528 of the side of vertical plate 527 and lower part
Level board 529, sampling gun driving motor 522 and sampling gun transmission mechanism are arranged on upper water plate 528, eject release piece 53
It can be arranged in up and down on lower horizontal plate 529 along height direction H, driving baffle 525 is located at upper water plate 528 and lower part
Between level board 529.Wherein, main body holding tank 5291, the main body 512 of sampling gun 51 be could be formed on lower horizontal plate 528
It can be received into the main body holding tank 5291 and be fixed on lower horizontal plate 528 by fasteners such as bolts, be therefore saved on
The arrangement space of pipette tips ejection releasing mechanism 5.In addition, being taken using bolt and nut drive mechanism as described above
Sample rifle driving motor 522 is mounted on the top of upper water plate 528, the output of the first screw rod 523 and sampling gun driving motor 522
Axis connection simultaneously is arranged as successively penetrating through upper water plate 528 and driving baffle 525 along height direction H, here, in order to which stabilization is led
To driving the moving in height direction H of baffle 525, optionally, sampling gun driving motor is located in upper water plate 528
The guide rod 5281 that perforation driving baffle 525 can be set on the position of 522 two sides, drives baffle 525 and guide rod
Guideway 5282 is provided between 5281.In addition, being also provided on upper water plate 528 for detecting driving baffle
The baffle position sensor 5283 of 525 position, for example, as shown in figure, baffle position sensor 5283 can be photoelectric transfer
Sensor, specifically can be using groove profile photoelectrical position sensor etc., here, baffle position sensor 5283 for example can detecte
High limit position of the baffle 525 in height direction H is driven, whether to detect the telescopic rod 511 of sampling gun 51 in complete
Stretching state.Correspondingly, it can also be provided on lower horizontal plate 529 for detecting driving baffle 525 in height direction H
Most sole extreme position position sensor, to detect whether the telescopic rod of sampling gun 51 is in fully retracted state.
When pipette tips as described above ejection releasing mechanism is adapted to pipette tips mobile device 41 as described above, such as Figure 12
It, can be by enabling pipette tips to eject releasing mechanism 5 along height side fixed pedestal 521 as described above to shown in Figure 15
It is arranged on pedestal 411 up and down to H, here, for example, pedestal 411 can be formed as gantry shape, at the top of pedestal 411
Releasing mechanism driving motor 412 is set, which passes through releasing mechanism screw-and-nut mechanism 413
It is connected with the transmission of fixed pedestal 521, that is, the screw rod of releasing mechanism screw-and-nut mechanism 413 extends and passes through along height direction H
The assembling stand 5211 of logical fixed pedestal 521, here, stablizing guiding pipette tips ejection 5 (specially assembling stand of releasing mechanism to play
5211) moving along height direction H as illustrated in fig. 15 can be in pedestal 411 positioned at releasing mechanism driving motor 412
The releasing mechanism guide frame 414 of movement for being oriented to pipette tips ejection releasing mechanism 5 is set on two side positions.The releasing mechanism
Guide frame 414 is identical as the structure of guide rod 5281 as described above and guideway 5282, and details are not described herein.In addition,
The fixed pedestal position sensor 415 of the position for detecting fixed pedestal 521 can be provided on pedestal 1, thus, it is possible to
High limit position of the detection pipette tips ejection releasing mechanism 5 in height direction H out.
To sum up, feature, mode of texturing and the function and effect etc. of pipette tips ejection releasing mechanism are described in detail, and above-mentioned retouch
It states and is mainly illustrated with embodiment of the pipette tips ejection releasing mechanism suitable for above-mentioned pipette tips mobile device 41, but this
Open it's not limited to that, and the pipette tips ejection releasing mechanism of structure as described above can be applicable to be used for the first work as described above
Make in the first mechanical arm 6 of region A and the second mechanical arm 7 for third working region C, it is in the case, mobile with pipette tips
Correspondingly, first mechanical arm 6 and second mechanical arm 7 can pass through well known motor and screw-and-nut mechanism to device 41 respectively
Mutually matched multiple groups transmission module, realize along being parallel to the longitudinal direction X1 of microscope carrier moving direction Z1 and perpendicular to the load
The adjustment position of the transverse direction X2 of platform moving direction Z1, and the lifting along height direction H.In this regard, omitting to the detailed of its
Explanation.
Optionally, the magnetic bead transfer device 42 as shown in Figure 12, Figure 20 and Figure 21, in nucleic acid extraction mechanism 4 as described above
Including magnetic bead transfer device fixing seat 421 of the microscope carrier transport establishment 1 on the position of sampling hammerhead 200 is arranged in, setting exists
Magnetic part actuator and magnetic part movable plate 422 in magnetic bead transfer device fixing seat 421, the magnetic part movable plate 422 are set
It sets in magnetic bead transfer device fixing seat 421, and connect with magnetic part actuator with can be along close to or far from sampling hammerhead 200
Direction it is flexible, magnetic part is magnet 423 and is mounted on magnetic part movable plate 422 close to the side of sampling gun 51.Here, will be as
In the case that the upper nucleic acid extraction mechanism 4 is suitable for sample processing device, the magnetic bead transfer device of magnetic bead transfer device 42
Fixing seat 421 can be set on the second working region B of microscope carrier transport establishment 1, such as can be set and in microscope carrier movement side
It is located at the front side of pipette tips mobile device 41 on Z1, is provided on the pipette tips ejection releasing mechanism 5 of pipette tips mobile device 41 more
In the case where a sampling gun 51, magnet 423 can also be positioned apart from the position of magnetic part movable plate 422 it is multiple,
In the sampling hammerhead 200 that loads on each magnet 423 and each sampling gun 51 it is opposite, can effectively ensure that each sampling hammerhead
200 can receive the effect of magnetic field force, and then guarantee the extraction quality of nucleic acid.Wherein, magnetic part actuator is adopted as a variety of
Reasonable structure, for example, magnetic part actuator can be telescoping cylinder, with drive magnetic part movable plate 422 towards close to or far from
The direction of sampling hammerhead 200 is flexible, or, optionally, magnetic part actuator includes magnetic part driving motor 426 and magnetic part
Bolt and nut mechanism, magnetic part driving motor 426 are arranged in magnetic bead transfer device fixing seat 421, magnetic part bolt and nut machine
The magnetic part screw rod 427 of structure and the output axis connection of the magnetic part driving motor 426, the magnetic part of magnetic part bolt and nut mechanism
Nut 428 is connect with magnetic part movable plate 422.As a result, the simple magnetic part actuator of structure as described above have stable drive,
The features such as use reliability is high.
In addition, pipette tips mobile device 41 as described above and magnetic bead transfer device 42 are suitable for sample as described above
In the case where processing equipment, such as in the second working region B, the step-by-step movement by microscope carrier 2 along nucleic acid extraction direction Z2 is moved
Come when realizing nucleic acid extraction operation, magnetic bead transfer device 42 can be on the Z2 of nucleic acid extraction direction positioned at magnetic bead transfer device 42
Downstream side, that is, specifically, for example, nucleic acid extraction direction Z2 is in contrast to the microscope carrier referred in sample processing device as described above
In the case where moving direction Z1, magnetic bead transfer device 42 is located at the front side of pipette tips mobile device 41 on microscope carrier moving direction Z1,
To avoid the moving along nucleic acid extraction direction Z2 in the second working region B of microscope carrier 2, at this point, since microscope carrier 2 is in nucleic acid extraction mistake
Be directed away from journey magnetic bead transfer device 42 direction movement, therefore, magnetic bead transfer device 42 can according to sampling hammerhead 200
Between actual range (such as can be according to moving distance of microscope carrier 2) come adjust in real time towards sampling hammerhead 200 stretch out
Degree is effectively ensured and provides magnetic force to sampling hammerhead 200, or carries out nucleic acid extraction mistake in the second working region B in microscope carrier 2
The overall distance that Z2 is moved along nucleic acid extraction direction in journey is smaller, and makes the magnetic force not shadow substantially applied to sampling hammerhead 200
Under the premise of ringing absorption magnetic bead, the magnetic part of magnetic bead transfer device 42 can be made repeatedly to stretch in the second working region B
Degree keeps identical.
Here, being moved in order to being steadily oriented to magnetic part movable plate 422, and convenient for assembly magnetic part movable plate
422 and magnetic bead device device fixing seat 421 optionally as shown in figure 21, be formed on magnetic part movable plate 422 along magnetic part
The long guiding hole 424 that the telescopic direction of movable plate 422 extends is provided on magnetic bead transfer device 42 and leads for being inserted into and being installed to
Positioning screw 425 into long hole 424, magnetic part movable plate 422 can by the cooperation of long guiding hole 424 and positioning screw 425
Telescopically it is arranged on magnetic bead transfer device 42.Here, mobile panel guide can be set in magnetic bead transfer device fixing seat 421
4211, magnetic part movable plate 422 can play dual guiding role by the cooperation of sliding block and mobile panel guide 4211.?
This can be set in magnetic bead transfer device fixing seat 421 to accurately control the stretching displacement of magnetic part movable plate 422
For detecting the magnetic part movable plate position sensor such as photoelectrical position sensor of the extended position of magnetic part movable plate 422
429。
To sum up, nucleic acid extraction mechanism 4 is turned by the lifting action and magnetic bead of the pipette tips mobile device 41 of structure as described above
The cooperation of the expanding-contracting action of moving device 42, the mode that bead complexes as described above follow sampling hammerhead 200 mobile always are held
Row nucleic acid extraction operation, and since the nucleic acid extraction of each sample only needs to can be realized as by a sampling hammerhead, thus
Sampling hammerhead consumptive material is saved, nucleic acid extraction cost is reduced.In addition, due to the nucleic acid extraction mechanism 4 to be fixed on microscope carrier fortune
Under stationary state in second working region B of transfer mechanism 1, cell cracking is successively executed by mobile sample container 3, is washed
The nucleic acid extractions operation such as wash, elute, thus convenient for operation nucleic acid extraction mechanism 4, so that the entire control of sample processing device is grasped
Tend to simplify as mode, be more easily implemented automation control.
In addition, being based on nucleic acid extraction mechanism 4 as described above, the disclosure also provides a kind of utilization nucleic acid as described above and mentions
The method for extracting nucleic acid of mechanism 4 is taken, the method for extracting nucleic acid includes: cleavage step S21, and sampling hammerhead 200 draws sample simultaneously
The sample is added in the cell pyrolysis liquid containing magnetic bead into sample container 3, so that obtaining adsorption after sample cracking has
The bead complexes of nucleic acid, sampling hammerhead draws the solution containing bead complexes, in magnetic part close to the side of sampling hammerhead 200
To stretching under magnetic state, sampling hammerhead 200 spues in addition to the bead complexes being adsorbed on 200 inner wall of sampling hammerhead
Solution；Washing step S22, under the demagnetizing state that magnetic part is retracted far from the direction of sampling hammerhead 200, sampling hammerhead 200 is inhaled
It samples the cleaning solution in this container 3 and executes compressing movement repeatedly, to clean bead complexes, later under for magnetic state,
Sampling hammerhead 200, which spues, removes the solution containing bead complexes；Elution step S23, under demagnetizing state, sampling hammerhead 200 is inhaled
It samples the eluent in this container 3 and executes compressing movement repeatedly, so that magnetic bead and nucleic acid separation in bead complexes,
Sampling hammerhead 200 draws the solution containing magnetic bead and nucleic acid；Nucleic acid extraction step S24, under for magnetic state, sampling hammerhead 200
By except the nucleic acid containing magnetic bead spues and saves to sample container 3, method for extracting nucleic acid is in sample container 3 with step-by-step movement
In traveling process, by the cooperation of the expanding-contracting action of the lifting action and magnetic bead transfer device 42 of pipette tips mobile device 41, successively
It is inserted into the holding tank of each sample container 3 and executes cleavage step S21, washing step S22, elution step S23 and core
Sour extraction step S24 and obtain nucleic acid.
Here, the process of nucleic acid extraction for ease of description, by each sample holding tank of sample container 3 be defined as
Under, that is, the sample holding tank arranged along microscope carrier moving direction Z1 is defined respectively and is used to accommodate the cell of cell pyrolysis liquid and splits
It solves liquid holding tank, the cleaning solution holding tank for accommodating cleaning solution, the eluent holding tank for accommodating eluent and is used for
Accommodate the nucleic acid holding tank of the nucleic acid extracted.As a result, when sample container 3 is moved along nucleic acid extraction direction Z2, pipette tips are mobile
The sampling hammerhead 200 loaded on device 41, which can be sequentially inserted into cell pyrolysis liquid holding tank, cleaning solution holding tank, eluent, to be held
Receive slot and nucleic acid holding tank.
As described above, the method for extracting nucleic acid of the disclosure extracts nucleic acid using paramagnetic particle method, can be realized high-throughput operation and
Automation, and the high-affinity for having used inorganic particle to be formed in conjunction with high molecular material due to paramagnetic particle method nucleic acid extraction
Composite magnetic microballoon (magnetic bead), the composite magnetic microballoon have both numerous characteristics of polymer microsphere and magnetic particle, therefore are not having
Having can be uniformly dispersed in the solution in the case where applying magnetic field, stablize, can be simple and quick in the case where applying magnetic field
Ground is separated with solution, is specifically bound with what is calculated so that the nucleic acid purity extracted is high, dense from there through composite magnetic microballoon
Degree is big, and with high security, it is low in cost and the advantages of convenient for being widely applied.
In the method for extracting nucleic acid of the disclosure, in sample container 3 to be filled by the way that pipette tips are mobile in step-by-step movement traveling process
The cooperation for setting 41 lifting action and the expanding-contracting action of magnetic bead transfer device 42 is sequentially inserted into the appearance of each sample container 3
It receives in slot and executes the nucleic acid extraction operation of interdependent ground.Specifically, the state of sampling hammerhead 200 is loaded in pipette tips mobile device 41
Under, sampling hammerhead 200 declines after drawing sample along height direction H and be inserted into sample container 3 accommodates cell pyrolysis liquid
In the cell pyrolysis liquid holding tank of magnetic bead, sample is expelled in cell pyrolysis liquid holding tank and executes and split by sampling hammerhead 200
Solve step S21.In cleavage step S21, sampling hammerhead 200 executes compressing movement so that sample and cell pyrolysis liquid repeatedly
It is sufficiently mixed, quiet to put a period of time so that sufficiently dissolution occurs for the cell of sample, later, sample is released after carrying out cell cracking
Nucleic acid out is mixed with magnetic bead, forms the solution for containing nucleic acid-bead complexes (hereinafter referred to as bead complexes).Sampling
Pipette tips 200 draw the cracking waste liquid containing bead complexes, in the case where magnetic part is for magnetic state, nucleic acid-bead complexes
It can be adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device 41 drives the discharge of sampling hammerhead 200 except magnetic bead is compound
Waste reaction solution except object.After completing cleavage step S21, pipette tips mobile device 41 drives sampling hammerhead 200 to rise and take off
From cell pyrolysis liquid holding tank, sample container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement later, so that sample container
The 3 cleaning solution holding tank for accommodating cleaning solution is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives sampling gun
First 200 decline and are inserted into cleaning solution holding tank, execute washing step S22.In washing step S22, in magnetic part
Under demagnetizing state, sampling hammerhead 200 executes compressing movement so that completing bead complexes and the washing of cleavage step S21 repeatedly
Liquid is sufficiently mixed, and achievees the effect that thoroughly to clean impurity.Later, it is useless to draw the washing containing bead complexes for sampling hammerhead 200
Liquid, in the case where magnetic part is for magnetic state, bead complexes are adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device
The 41 driving discharges of sampling hammerhead 200 are in the scrub raffinate to cleaning solution holding tank in addition to bead complexes.Here, for magnetic bead
The washing step S22 of compound can be executed repeatedly, such as twice repeatedly, in the case, can also be right in sample container 3
It is provided with multiple such as two cleaning solution holding tanks with answering.After completing to the washing step S22 of bead complexes, pipette tips are moved
Dynamic device 41 drives sampling hammerhead 200 to rise and be detached from cleaning solution holding tank, and sample container 3 is along nucleic acid extraction direction Z2 later
It is mobile with step-by-step movement so that the eluent holding tank for accommodating eluent of sample container 3 be located at sampling hammerhead 200 just under
Side, pipette tips mobile device 41 drive sampling hammerhead 200 to decline and are inserted into eluent holding tank, execute elution step S23.?
In elution step S23, under the demagnetizing state of magnetic part, sampling hammerhead 200 executes compressing movement so that completing to wash repeatedly
The bead complexes and eluent for washing step S22 are sufficiently mixed, and achieve the effect that the extracting nucleic acid from magnetic bead.Sampling hammerhead 200
Draw the solution containing magnetic bead and nucleic acid.After completing to execute elution step S23, pipette tips mobile device 41 drives sampling hammerhead
200 rise and are detached from eluent holding tank, and sample container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement later, so that sample
The nucleic acid holding tank of this container 3 is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives under sampling hammerhead 200
It drops and is inserted into nucleic acid holding tank, execute nucleic acid extraction step S24.In nucleic acid extraction step S24, in the confession of magnetic part
Under magnetic state, the discharge of sampling hammerhead 200 is in the nucleic acid solution to nucleic acid holding tank in addition to magnetic bead.Thus entire nucleic acid is completed to mention
It is taken as industry.
To sum up, through method for extracting nucleic acid as described above during nucleic acid extraction, nucleic acid-magnetic bead as described above is multiple
It closes object and follows the movement of sampling hammerhead 200 always, therefore the nucleic acid extraction of each sample is only needed through a sampling hammerhead energy
It is enough to realize, sampling hammerhead consumptive material is thereby saved, nucleic acid extraction cost is reduced.In addition, during nucleic acid extraction, due to rifle
41 drive sampling hammerheads 200 of head moving device can make magnetic bead transfer device 42 exist along height direction H shift position
In microscope carrier transport establishment 1 it is relatively fixed in the state of, by the expanding-contracting action of the magnetic part of magnetic bead transfer device 42 be convenient for
Bead complexes provide magnetic force or removal magnetic force so that nucleic acid extraction simple operation, reduce nucleic acid extraction mechanism with
And the manufacture and processing cost of sample processing device.
Optionally, in cleavage step S21, after sampling hammerhead 200 draws the solution containing bead complexes, further include
Protein digestibility step S25, under demagnetizing state, sampling hammerhead 200 spues the solution containing bead complexes to the sample
It in protein digestibility enzyme solutions in this container 3 and executes compressing repeatedly to act, with the protein in digestion solution, Zhi Hou
The solution containing bead complexes is removed under magnetic state, the sampling hammerhead 200 spues, to complete the cleavage step S21.
Here, can be set between cell pyrolysis liquid holding tank and cleaning solution holding tank for holding in sample container
It receives the protein digestibility enzyme solutions holding tank containing protein kinase, accommodates splitting containing bead complexes in sampling hammerhead 200
In the state of liquid waste solution, pipette tips mobile device 41 drives sampling hammerhead 200 to rise and be detached from cell pyrolysis liquid holding tank, later sample
This container 3 is moved along nucleic acid extraction direction Z2 with step-by-step movement, so that sample container 3 accommodates the egg containing protein kinase
White matter digestion enzyme solutions holding tank is located at the underface of sampling hammerhead 200, and pipette tips mobile device 41 drives sampling hammerhead 200 to decline
And be inserted into protein digestibility enzyme solutions holding tank, execute protein digestibility step S25.In protein digestibility step S25
In, under the demagnetizing state of magnetic part, sampling hammerhead 200 executes compressing movement so that containing the magnetic for completing cell cracking repeatedly
The cracking waste liquid of pearl compound is sufficiently mixed with the protein digestibility enzyme solutions containing protein kinase, reaches the egg in digestion solution
The effect of white matter.Later, sampling hammerhead 200 draws the waste reaction solution containing bead complexes, in the case where magnetic part is for magnetic state,
Bead complexes are adsorbed on the inner wall of sampling hammerhead 200, at this point, pipette tips mobile device 41 drives the discharge of sampling hammerhead 200 to remove
In waste reaction solution to protein digestibility enzyme solutions holding tank except bead complexes, to complete cleavage step S21 and execute
Next step, that is, washing step S22.By step as described above, nucleic acid extraction quality can be further increased, guarantees sample
The accuracy of processing.
According to another aspect of the present disclosure, following microscope carrier and microscope carrier fortune can be applicable in the microscope carrier processing equipment of the disclosure
The cooperation assembly of transfer mechanism.Referring to as shown in Figure 22 to Figure 29, wherein the cooperation assembly of the disclosure, which uses, passes through other structures
The microscope carrier passive type driving method for driving microscope carrier 2 to move, that is, microscope carrier and the cooperation assembly of microscope carrier transport establishment include 2 He of microscope carrier
Microscope carrier transport establishment 1, is provided with microscope carrier driving mechanism in microscope carrier transport establishment 1, microscope carrier 2 by microscope carrier driving mechanism movably
It is arranged in microscope carrier transport establishment 1, the working region locating in microscope carrier transport establishment 1 of microscope carrier 2 can be adjusted.Here, carrying
The passive type for driving microscope carrier 2 to move by microscope carrier driving mechanism is used in platform processing equipment and cooperation assembly as described above
Microscope carrier driving method here, microscope carrier driving mechanism can use any suitable arragement construction, such as can be independent one
Mechanism also may include multiple independent mechanisms, each mechanism can independent realization driving microscope carrier 2 in microscope carrier transporter
The function of moving on structure 1 for another example can be with for example, the function of movement of the driving microscope carrier 2 in microscope carrier transport establishment 1 may be implemented
The function that driving microscope carrier 2 is gone up and down in microscope carrier transport establishment 1 is realized, as long as microscope carrier driving mechanism can be realized driving microscope carrier 2 and transport
Function that is dynamic and adjusting the working region locating in microscope carrier transport establishment 1 of microscope carrier 2.By structure as described above, i.e.,
In sample process, microscope carrier 2 can be made to move in microscope carrier transport establishment 1 by microscope carrier driving mechanism, thus execute sample
During present treatment so that microscope carrier 2 be sequentially located in corresponding each working region of microscope carrier transport establishment 1 and with it is corresponding
Mechanical arm cooperation, thereby executing corresponding sample process work, thus the above-mentioned cooperation assembly of the disclosure and it is existing in pass through machine
The movement of tool arm is compared come the mode successively to fixed microscope carrier realization sample process, enables to the movement road of corresponding mechanical arm
Diameter and operation are more simple, and the disclosure is by movement of the microscope carrier 2 relative to microscope carrier transport establishment 1, multiple microscope carriers 2 according to
In the case where secondary execution sample process, so that multiple microscope carriers 2 successively move in microscope carrier transport establishment 1 and in each working regions
It is interior that only by a mechanical arm multiple microscope carriers 2 can successively be executed with corresponding work, to save whole manufacture and processing
Sample process efficiency is also improved while cost.
Optionally, as shown in figure 22, microscope carrier driving mechanism includes for driving microscope carrier 2 to move along microscope carrier moving direction Z1
Microscope carrier driven in translation structure 12, microscope carrier driven in translation structure 12 include the first driving motor 121 and first driving motor 121
The first connected transmission component of output shaft transmission and connect and can separatably cooperate with microscope carrier 2 with the first transmission component
Movable trolley 122, the first transmission component is used to the rotary motion of the first driving motor 121 being converted to movable trolley 122
Linear movement, to enable to movable trolley 122 to adjust the institute in microscope carrier transport establishment 1 of microscope carrier 2 by the cooperation with microscope carrier 2
The working region at place.Here, the first transmission component can use the structure of various reasonable, for example, optionally, the first transmission component
It can be V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission matching mechanism, thus, it is possible to improve transmission
Reliability and transmission efficiency.In structure as described above, movable trolley 122 and the departing fit system of microscope carrier 2 can be with
Various structures are used, for example, can be by the way that telescopic stretching structure is arranged on movable trolley 122, to pass through stretching structure
It stretches out and is inserted into the mode on microscope carrier 2 to realize the relatively fixed of movable trolley 122 and microscope carrier 2, retracted by stretching structure
And the mode of microscope carrier 2 is detached to realize the relative motion of movable trolley 122 and microscope carrier 2；It for another example, can be by movable trolley
The clamping element that can clamp is set on 122, movable trolley 122 and microscope carrier 2 are realized in a manner of clamping microscope carrier 2 by clamping element
It is relatively fixed, the relative motion of movable trolley 122 and microscope carrier 2 is realized in such a way that clamping element discharges microscope carrier 2.But disclosure is simultaneously
It is not limited to this, movable trolley 122 can carry out reasonable design with the departing fit system of microscope carrier 2 according to actual needs.It is logical
Structure as described above is crossed, that is, starts the first driving motor 121 and outputs it the rotary force of power axis and pass through the first transmission component
Transmit and be converted to the linear movement of movable trolley 122, reliable mobile of the guarantee activity trolley 122 in microscope carrier transport establishment 1,
And can steadily drive microscope carrier 2 to move to each working region, it ensure that the transportation of microscope carrier 2.
Optionally, the first transmission component is V belt translation matching mechanism, and is passed including the first transmission belt 123, setting first
Move 123 side of band and with the first driving wheel 124 of the output axis connection of the first driving motor 121 and setting in the first transmission belt
First driven wheel 125 of 123 other sides, movable trolley 122 are mounted on the first transmission belt 123.Wherein, V belt translation matching mechanism
Have many advantages, such as that stable drive, buffering absorbing, structure is simple, at low cost, working service is convenient, in microscope carrier transport establishment 1, carries
In the longer situation of the moving range of platform 2, only allowed for by the V belt translation matching mechanism of simple structure as described above
First transmission belt 123 is reliable and easily drive activity trolley 122 realizes flexible movement in moving range.
In order to remain movable trolley 122 during 123 drive activity trolley 122 of the first transmission belt is mobile
Do linear movement, and avoid because the first transmission belt 123 is too long lead to deformation due to there is microscope carrier 2 movement routine change, can
Selection of land, as shown in Figure 24 to Figure 29, the downside in microscope carrier transport establishment 1 positioned at the first transmission belt 123 is provided with moves along microscope carrier
The trolley guide rail 126 that direction Z1 extends, movable trolley 122 are fixed in the downside band part of the first transmission belt 123, movable trolley
122 bottom is provided with the second pulley component 127 slideably cooperated with trolley guide rail 122.Movable trolley 122 passes through second
Pulley assembly 127 rollably supports on trolley guide rail 126, so that trolley guide rail 126 can be smoothly directing to movable trolley
122 linear movement on microscope carrier moving direction Z1, simultaneously, additionally it is possible to cooperate more flexible by rolling and easily realize
Movable the moving along microscope carrier moving direction Z1 of trolley 122.
Here, optionally, such as scheming for the installation reliability being effectively ensured between movable trolley 122 and trolley guide rail 126
Shown in 30, the bottom of movable trolley 122, second pulley peace are arranged in by second pulley mounting base 128 for second pulley component 127
Dress seat 128 is located at 123 downside of the first transmission belt and is supported on trolley guide rail 126 by second pulley component 127.But the disclosure
It's not limited to that, and alternatively, the bottom of movable trolley 122 can be formed directly with for for the first transmission belt
The transmission belt mounting groove that 123 lower portion passes through, second pulley component 127 can be mounted directly or be formed in movable trolley
122 bottom.
Optionally, stretching structure 8 is telescopically provided on movable trolley 122, microscope carrier 2 corresponds to movable trolley 122
The locking structure 21 being mutually locked for cooperating with stretching structure 8 is provided on the side wall of side.Here, for stretching structure 8
The arrangement of various reasonable can be used with the structure of locking structure 21, as long as can by the flexible of stretching structure 8
Cooperate or separate with locking structure 21, so that microscope carrier 2 is locked on movable trolley 122 or is detached from from movable trolley 122.
During sample process in the case where a certain working region for needing for microscope carrier 2 to be moved to microscope carrier transport establishment 1, activity
Trolley 122 drives microscope carrier 2 to be moved to a certain working region by the cooperation of stretching structure 8 and locking structure 21, wherein
In the case that microscope carrier 2 is multiple, movable trolley 122 can pass through the cooperation of stretching structure 8 and locking structure 21 and disengaging
Mode successively drives multiple microscope carriers 2 to be moved to corresponding working region, so that microscope carrier 2 is consecutively carried out sample process
Operation improves the operating efficiency of whole sample process.Here, for example, stretching structure 8 can use electric cylinder, hydraulic cylinder, gas
The flexible cylinder structures such as dynamic cylinder, for another example, stretching structure 8 can also be using screw pairs, rack-and-pinion, worm gears such as motor and feed screw nuts
The matching mechanism of the drive mechanisms such as worm screw is matched in such a way that the driving by motor is so that each bar is flexible with locking structure 21
It closes.
Here, for another example, optionally, as shown in Figure 30 to Figure 32, stretching structure 8 includes the second magnet plunger 81, the second electromagnetism
Bar 81 has lockup state and unlocked state, in lockup state, the second magnet plunger 81 power-off, so that the second magnet plunger 81 pushes away
Bar is stretched out and is snapped on locking structure 21 and locking microscope carrier 2 and movable trolley 122；In unlocked state, the second magnet plunger 81 is logical
Electricity, to make pusher retracted unlock microscope carrier 2 and movable trolley 122, locking knot by the magnetic force generated in the second magnet plunger 81
Structure 21 is formed to have lock groove or locking hole with the shape of the second magnet plunger 81 cooperation.Thus, it is possible to make movable table vehicle
Reliable locking is realized between 122 and microscope carrier 2, and movable trolley 122 is able to drive microscope carrier 2 and is moved to exact position, passes through
Structure as described above can effectively improve the operational reliability of movable trolley 122.In addition, in order to further increase movable table
The locking reliability of vehicle 122 and microscope carrier 2, as shown in Figure 30 to Figure 32, stretching structure 8 can also include electromagnet 82, the electricity
Magnet 82 be two and be separately positioned on the movable trolley 122 positioned at the position of 81 two sides of the second magnet plunger on, load
Platform 2, which corresponds on the side wall of the electromagnet 82, is formed with electromagnet latch 28 for cooperating with the electromagnet 82,
The lockup state, the electromagnet 82 is powered and the magnetic force by generating is adsorbed onto the electromagnet latch 28, with
Microscope carrier 2 described in locking and the movable trolley 122；In the unlocked state, the electromagnet 82 powers off and is detached from the electromagnetism
Iron latch 28, to unlock the microscope carrier 2 and the movable trolley 122.Here, electromagnet 82 can use magnechuck etc.,
Pass through structure as described above, that is, the second magnet plunger 81 and electromagnet 82 it is synchronous stretch out or it is synchronous retract by way of with
Microscope carrier 2 realizes secure ratcs or unlock.
Optionally, described to match as shown in Figure 22 to Figure 27, Figure 32 and Figure 33 according to the first embodiment of the disclosure
Closing assembly includes the microscope carrier location structure, and the microscope carrier location structure includes 22 He of the first positioning seat being arranged on microscope carrier 2
The second positioning seat 15 in microscope carrier transport establishment 1 is set, is formed with the first positioning region, the second positioning seat on the first positioning seat 22
Multiple second positioning regions for cooperating or separating with the first positioning region are positioned apart from along microscope carrier moving direction Z1 on 15, the
It, can be with corresponding second positioning when one positioning portion is located at the position opposite with any one the second positioning region in the second positioning region
Portion cooperates and positions.As described above, microscope carrier 2 in microscope carrier transport establishment 1 along microscope carrier moving direction Z1 moving process, first is fixed
When position portion is placed exactly in an opposite position in the second positioning region in the second positioning regions multiple in microscope carrier transport establishment 1, the
One positioning region and opposite second positioning region cooperation and by microscope carrier 2 navigate to the corresponding working region of microscope carrier transport establishment 1 (such as
First working region A, the second working region B or third working region C etc.), the reliability of positioning of microscope carrier 2 is effectively ensured, thus
Execute corresponding sample process operation (such as the first working region A execute sample dispensing operation, held in the second working region B
Row nucleic acid extraction operation executes detection of nucleic acids operation in third working region C), the first positioning region is determined with opposite second later
Position portion occur separation and make microscope carrier 2 relative to microscope carrier transport establishment 1 be in move freely state, so that microscope carrier 2 moves again
To subsequent work region, and pass through the behaviour repeatedly of cooperation and separation between the first positioning region as described above and the second positioning region
Make, and then favorably accomplishes sample process operation.
Here, the fit system of the first positioning region and the second positioning region can use the arragement construction of various reasonable, for example,
It specifically can for example be used electronic when using flexible fit system using fit systems such as flexible, rotation, clampings
A variety of stretching structures such as cylinder, hydraulic cylinder or it is also possible to the stretching structure of feed screw nut form, by the flexible of magnetostrictive
Structure etc..It for another example, can be by as one of the first positioning region and the second positioning region when use is rotatably assorted mode
It rotates the rotation of executive item and is caught in the form of another one to realize the cooperation or separation of the first positioning region and the second positioning region.Again
Such as, using fit system is clamped, the second positioning can be clamped by can be used as the clamping piece of the first positioning region
The form in portion is realized.
In an embodiment of the disclosure, in order to enable the fit form of the first positioning region and the second positioning region is simple
And be easy to arrange on microscope carrier 2 and microscope carrier transport establishment 1, optionally, one of the first positioning region and the second positioning region are formed
For locating slot 221 or location hole, another one is formed as extensible member, which is configured to be inserted into and navigates to locating slot
It 221 or positioning hole or is detached from from locating slot 221 or location hole.Here, optionally, as shown in Figure 23 and Figure 32, the second positioning
Portion is the first magnet plunger 151, and the first positioning region is locating slot 221, and the first magnet plunger 151 has working condition and inoperative shape
State, in working condition, the first magnet plunger 151 power-off, so that the push rod of the first magnet plunger 151 stretches out and is adsorbed onto locating slot
In 221, in off working state, the first magnet plunger 151 is powered, to make push rod by the magnetic force generated in the first magnet plunger 151
It retracts and is detached from locating slot 221.That is, locating slot 221 is formed on the first positioning seat 22 of microscope carrier 2, by the first magnet plunger 151
It is arranged on the second positioning seat 15 of microscope carrier transport establishment 1, can be convenient due to locating slot 221 and is easily worked into microscope carrier 2
On, so that 2 overall structure of microscope carrier is simple and can move flexibly and easily in microscope carrier transport establishment 1.Wherein, the first electromagnetism
Bar 151 is to realize the linear reciprocation of itself push rod using the actuation and release of electromagnet dynamic iron core and static iron core with push rod
The extensible member of movement.When being placed exactly in 2 moving process of microscope carrier in multiple first magnet plungers 151 with microscope carrier transport establishment 1
When the corresponding position of one, i.e., in the operating condition, corresponding first magnet plunger 151 powers off, so that push rod is stretched out and is inserted into
In locating slot 221, microscope carrier 2 is thus navigated into corresponding working region in microscope carrier transport establishment 1；In the corresponding working region
After the interior corresponding sample process operation of completion, i.e., in a non-operative state, the first magnet plunger 151 is powered and is generated by internal
Magnetic force and make pusher retracted so that microscope carrier 2 positioning is released from microscope carrier transport establishment 1 and in free movement shape
State.This first magnet plunger 151 small and limited sky in microscope carrier transport establishment 1 of installation space shared by it on the second positioning seat 15
It is interior reasonably to arrange multiple first magnet plungers 151, and have many advantages, such as that maintenance cost is low, reliable for operation.But the disclosure
It's not limited to that, can be using structures such as electric pushrods as the alternative of the first magnet plunger 151.
Optionally, the second positioning seat 15 is separately provided for detection first on the position of each second positioning region
The position sensor of the position of positioning seat 22 detects the first positioning with a position sensor in multiple position sensors
When the position of seat 22, so that corresponding to the second positioning region of a position sensor and first positioning region in the second positioning region
Cooperation.Here, position sensor can use various structures, such as edge carries in microscope carrier transport establishment 1 in order to not influence microscope carrier 2
The movement of platform moving direction Z1, position sensor can be non-contact position sensor, such as optionally, position sensor is
Photoelectrical position sensor, the Mechanical Contact that this photoelectrical position sensor eliminates between microscope carrier 2 and microscope carrier transport establishment 1 are asked
Topic avoids that movement interference occurs to microscope carrier 2, and also has the advantages that service life length, high reliablity.Here, optionally, extremely such as Figure 22
Shown in Figure 26, position sensor is groove profile photoelectrical position sensor 16, and the slot of groove profile photoelectrical position sensor 16 is transported towards microscope carrier
The inside opening of transfer mechanism 1 is formed with the slot for passing through groove profile photoelectrical position sensor 16 on first positioning seat 22
Block 222.Here, the slot of groove profile photoelectrical position sensor 16 can be U-lag, transmitter and receiver are located at U-shaped
The two sides of slot simultaneously form optical axis, microscope carrier 2 in microscope carrier transport establishment 1 along microscope carrier moving direction Z1 motion process, when microscope carrier 2
The first positioning seat 22 on block 222 by between transmitter and receiver and when blocking optical axis, groove profile optoelectronic position sensing
Device 16 generates the on-off model for detecting block 222, according to the signal that trough type photoelectric sensor 16 detects, microscope carrier transporter
It is located on structure 1 and matches with the second positioning region of 16 corresponding position of groove profile photoelectrical position sensor with the first positioning region on microscope carrier 2
It closes, so that microscope carrier 2 is navigated to the corresponding working region of microscope carrier transport establishment 1, here, the second positioning region is as described above the
In the case that one magnet plunger 151 and the first positioning region are locating slot 221, the first magnet plunger 151 powers off and push rod is stretched out simultaneously
It is inserted into locating slot 221, thus reliable location microscope carrier 2.But it's not limited to that for the disclosure, and position sensor can also use
Other structures, for example, position sensor can be magnetic sensitive position sensor, this can equally eliminate microscope carrier 2 and microscope carrier transport
The Mechanical Contact of mechanism 1, and reliability is higher.
On the basis of microscope carrier location structure as described above, for the specific of the first positioning seat 22 and the second positioning seat 15
Installation site can select according to the actual situation, for example, in order to enable microscope carrier 2 and the fit structure of microscope carrier transport establishment 1 more
Adduction reason and realization reliable location, optionally, the first positioning seat 22 is arranged on the side wall of microscope carrier 2, the setting of the second positioning seat 15
On the side inner sidewall of microscope carrier transport establishment 1.
Here, can by movable trolley 122 as described above according to the microscope carrier 2 of first embodiment as described above
It is movably arranged in microscope carrier transport establishment 1 along microscope carrier moving direction Z1.Here, driving microscope carrier 2 to exist by movable trolley 122
It is moved in microscope carrier transport establishment 1 along microscope carrier moving direction Z1, and passes through the first positioning region of microscope carrier 2 and microscope carrier transport establishment 1
On the second positioning region detection to 2 position of microscope carrier of cooperation and position sensor, so that microscope carrier 2 is navigated to microscope carrier transporter
Structure 1 executes correspondingly sample process operation to areas inside operating, and the control precision to microscope carrier 2 has thus been effectively ensured.
Further optionally, described according to the second embodiment of the disclosure as shown in Figure 22 to Figure 27, Figure 33 to Figure 36
Cooperation assembly includes the microscope carrier guide frame for being oriented to movement of the microscope carrier 2 in microscope carrier transport establishment 1, microscope carrier guide frame packet
It includes the microscope carrier guide rail 17 of 1 two sides of microscope carrier transport establishment and slidably engages and be arranged in 2 bottom two of microscope carrier with microscope carrier guide rail 17
The two sides being located on microscope carrier moving direction Z1 in microscope carrier transport establishment 1 are arranged in the first pulley component 23 of side, microscope carrier guide rail 17
On inner wall.Here, microscope carrier guide frame may include the microscope carrier 2 such as flowering structure.
That is, for microscope carrier guide frame microscope carrier 2 may include for in microscope carrier transport establishment 1 be located at microscope carrier movement side
The first pulley component 23 that microscope carrier guide rail 17 on two sides inner wall on Z1 cooperates, first pulley component 23 are arranged in microscope carrier
2 two sides of the bottom, the moving along microscope carrier moving direction Z1 on microscope carrier guide rail 17 of microscope carrier 2, each first pulley group can be oriented to
Part 23 has two groups of first pulleys at the interval X2 in transverse direction, and the wheel shaft of each first pulley is vertically disposed on microscope carrier 2,
Two groups of first pulleys of each first pulley component 23 can adjust two group first by X2 in transverse direction by position adjustment structure
Horizontal spacing between the wheel shaft of pulley, wherein transverse direction X2 is arranged perpendicularly to microscope carrier moving direction Z1.Here, position
Adjustment structure can use the arragement construction of various reasonable, as long as can be realized two groups of first pulleys of adjustment X2 tune in transverse direction
The function of whole spacing.It wherein, can be by making any one group first cunning in two groups in each first pulley component 23
It takes turns and adjusts position along the direction close to or far from another group of first pulley on transverse direction X2, or can also be by making two
Group first pulley adjusts position along direction close to each other or separate on transverse direction X2, so as to adjust two group first
Thus microscope carrier 2 is being installed under the use state in transport establishment 1 by the spacing between pulley, can effectively ensure that two group
One pulley is bonded with the two sides inner wall of the microscope carrier guide rail 17 of microscope carrier transport establishment 1, plays reliable guiding microscope carrier 2 in transport establishment
Movement on 1, meanwhile, by position adjustment structure adjust two groups of first pulleys between spacing, can with a variety of different structures,
The microscope carrier guide rail 17 of various sizes of microscope carrier transport establishment 1 cooperates, and thus has extensive versatility, mass production may be implemented
And reduce the exploitation and manufacturing cost that first pulley component 23 is manufactured for the microscope carrier guide rail 17 of different structure.In addition, first
Pulley assembly 23 can pass through the transverse direction of subsequent adjustment first pulley component 23 because long-time service causes in the case where wearing
Position come compensate abrasion loss without replace first pulley component 23, thereby save the maintenance of microscope carrier 2 and microscope carrier guide frame
Cost and the recycling property for improving first pulley component 23.
Here, optionally, such as scheming to quickly and easily adjust the horizontal spacing between the wheel shaft of two groups of first pulleys
Shown in 36, two groups of first pulleys are respectively that the first fixed pulley 232 and the first movable pulley 233, the first movable pulley 233 pass through
Position adjustment structure can adjust position by X2 in transverse direction, and two limit adjustment position of the first movable pulley 233 is located at
The two sides of first fixed pulley 232.As a result, only by adjusting the first movable pulley 233 on the bottom of microscope carrier 2 in transverse direction
The position of X2 enables to the first movable pulley 233 and the first fixed pulley 232 to paste with the two sides inner wall of microscope carrier guide rail 17
It closes, while the position of the first movable pulley 233 can also be adjusted according to actual needs, be located at the first fixed pulley 232
Left or right side, or arrange structure in a row with the first fixed pulley 232, so as to adjust two sides first pulley component 23 it
Between spacing.It specifically may be adjusted so that the spacing between two sides first pulley component 23 becomes smaller, also adjustable is to make
Spacing between two sides first pulley component 23 becomes larger, allow first pulley component 23 the bottom of microscope carrier 2 neatly
It realizes adjustment, and then can adapt to the microscope carrier guide rail 17 of a variety of different spacing.
In order to enable the structure of first pulley component 23 is more rationalized and can be led more stablely on microscope carrier guide rail 17
Movement to microscope carrier 2, optionally, the first fixed pulley 232 and the first movable pulley 233 are multiple, and respectively along microscope carrier 2
Longitudinal direction X1 arrangement, longitudinal direction X1 are aligned parallel to microscope carrier moving direction Z1, the first fixed pulley 232 and the first activity
Pulley 233 is alternately arranged on longitudinal direction X1.Wherein, multiple first movable pulleys 233 can be by connecting lever linkage, thus
In the case where adjusting the horizontal spacing between the first movable pulley 233 and the first fixed pulley 232, connecting rod can be passed through
Thus the effect of the adjustment position of the first movable pulley 233 is improved in the position for designing multiple first movable pulleys 233 of synchronous adjustment
Rate improves operation ease.
Here, in order to enable microscope carrier overall structure is simpler, molding easy to process, optionally, such as Figure 36 and Figure 37 institute
Show, first pulley component 23 is mounted on the bottom of microscope carrier 2 by first pulley mounting base 231, and position adjustment structure includes being formed
First pulley in first pulley mounting base 231 installs block hole 234, and the be arranged on the first movable pulley 233
The wheel shaft 2331 of one pulley fastening structure, the first movable pulley 233 can be inserted into first with adjusting position by X2 in transverse direction
Pulley is installed in block hole 234, and is positioned by first pulley fastening structure.Here, first pulley fastening structure can use
Various structures, as long as can be realized in first pulley installation block hole 234, X2 adjusts position and enables in transverse direction
First movable pulley 233 is relatively fixed to the function of the bottom of microscope carrier 2.Optionally, as shown in figure 38, first pulley fastens
Structure includes the screw thread 235 being formed on 2331 outer peripheral surface of wheel shaft of the first movable pulley 233 and with connecting with screw thread 235
The fastening nut 236 of internal screw thread is formed in first pulley mounting base 231 and is connected to and uses with first pulley installation block hole 234
In the fastening nut mounting groove 238 for accommodating fastening nut 236, the fastening nut 236 is formed as cylindrical body and the internal screw thread
2361 arranged off-centres are on the fastening nut 236.Wherein, it can be set between the first movable pulley 233 and wheel shaft 2331
First movable pulley bearing 2332.When adjusting the position of the first movable pulley 233, can first be unclamped from fastening nut 236
The wheel shaft 2331 of first movable pulley 233 enables the wheel shaft 2331 of the first movable pulley 233 logical in first pulley mounting base
Movement in hole 234 specifically makes the wheel shaft 2331 connecting with eccentric internal screw thread first by rotating adjusting nut 236
Pulley installs movement in block hole 234, to adjust the position of the first movable pulley 233 on transverse direction X2, is being adjusted in place
Tighten the internal screw thread 2361 of fastening nut 236 and the screw thread 235 of wheel shaft 2331 again afterwards, it is thus that the first movable pulley 233 is reliable
Ground is locked at the bottom of microscope carrier 2, so that it is more simple to adjust operation.But it's not limited to that for the disclosure, first pulley component 23
Other reasonable structures can also be used, for example, the first movable pulley 233 is configured to screw bolt-type pulley, at this point, first is sliding
Wheel mounting base could be formed with the threaded hole being threadedly coupled with the screw thread of the screw bolt-type pulley, and this structure equally also can
Realize the function that the first movable pulley 233 is fastened after the position of the first movable pulley 233 adjusts.
Optionally, it as shown in Figure 30 and Figure 37, is circumferentially formed on the outer peripheral surface of first pulley for being inserted into and cooperating
Microscope carrier guide rail mating groove 237 on to the microscope carrier guide rail 17 of microscope carrier transport establishment 1.Herein optionally, the load of microscope carrier transport establishment 1
Platform guide rail 17 can be formed as being formed with both ends open along guide rail extending direction at the top of microscope carrier guide rail 17 such as flowering structure
Microscope carrier mating groove 171 is formed with opposite microscope carrier limit outstanding along guide rail extending direction on the two sides inner wall of microscope carrier mating groove 171
Position protrusion 172, microscope carrier guide rail mating groove 237 is formed as the shape cooperated with microscope carrier retention bead 172.Here, for example, microscope carrier limits
Position protrusion 172 is formed as semicylinder shape, at this point, microscope carrier guide rail mating groove 237 is formed as cooperating with microscope carrier retention bead 172
Semicircular arc groove, that is, first pulley can use concave groove pulley.As a result, in first pulley component 23 from microscope carrier guide rail
When 17 open at one end is installed on microscope carrier guide rail 17, pass through the cooperation of microscope carrier mating groove 171 and microscope carrier retention bead 172, energy
It enough effectively prevent first pulley component 23 to be detached from from microscope carrier guide rail 17, while also acting as the effect of certain supporting platform 2.But this
Open it's not limited to that, and first pulley can be formed as other structures, for example, first pulley is configured to common flat table
Face type pulley etc..
Optionally, it is provided on microscope carrier 2 for microscope carrier 2 to be navigated to each working region in microscope carrier transport establishment 1
Microscope carrier positioning region.Here, microscope carrier positioning region can be using the structure of the first following positioning seat, by transporting with following microscope carrier
The cooperation of the second positioning seat in mechanism 1 and make microscope carrier 2 be accurately positioned in each working region of microscope carrier transport establishment 1 and
Corresponding sample process operation is executed, the reliability of positioning to microscope carrier 2 has been effectively ensured.
To sum up, the microscope carrier guide frame of the microscope carrier for being provided with first pulley component including second embodiment as described above
All features and function and effect as described above comprising being provided with the microscope carrier 2 of first pulley component, herein in order to avoid repeating,
Details are not described herein.
It is described with reference to as shown in Figure 32 to Figure 35 and Figure 39 to Figure 42 in addition, according to the third embodiment of the disclosure
Microscope carrier and the cooperation assembly of microscope carrier transport establishment may include following microscope carrier.That is, microscope carrier 2 may include fixing seat 24 and can stretch
The sliding seat 25 in fixing seat 24 is arranged in contracting ground, and the container for accommodating sample container 3 is provided in sliding seat 25 and is accommodated
Slot 251.As a result, by microscope carrier 2 as above, under the use state cooperation assembly as described above, fixing seat 24 is installed
It, herein can be by making sliding seat 25 relative to the expanding-contracting action of fixing seat 24, so that sliding seat 25 in microscope carrier transport establishment 1
On container holding tank 251 relative to microscope carrier transport establishment 1 expose and convenient for execute sample container 3 pick-and-place movement, thus
Improve the pick-and-place efficiency and speed of sample container.
Here, the structure of various reasonable can be used for the fit structure between sliding seat 25 and fixing seat 24, as long as
Sliding seat 25 is enabled to make container holding tank 251 be exposed to microscope carrier transport establishment 1 relative to 24 relative motion of fixing seat
Outside.For example, optionally, microscope carrier 2 includes using in order to reliably be oriented to expanding-contracting action of the sliding seat 25 in fixing seat 24
In the sliding seat guidance set 26 that guiding sliding seat 25 is slided along the first telescopic direction P relative to fixing seat 24.Here, sliding seat
25 the first telescopic direction P can be used perpendicular to microscope carrier moving direction Z1, sliding seat guidance set 26 and be slidably matched, roll
The modes such as dynamic cooperation, for example, optionally, sliding seat guidance set 26 may include mutual using being slidably matched
The guide groove 261 and guide protrusions 262 of cooperation, guide groove 261 are arranged in sliding seat 25 and fixing seat 24 on any one, and
Extend along the first telescopic direction P, guide protrusions 262 are arranged in sliding seat 25 and fixing seat 24 in remaining one.For example, as schemed
Shown in 35, it can be formed with guide groove 261 in the two sides of fixing seat 24, accordingly, the two sides of sliding seat 25 are formed with and are oriented to
The guide protrusions 262 that slot 261 cooperates, play stable guiding activity by the cooperation of guide protrusions 262 and guide groove 261 as a result,
Expanding-contracting action of the seat 25 in fixing seat 24.For another example, it in the case that sliding seat guidance set 26 is using cooperation is rolled, can use
Such as flowering structure.That is, sliding seat guidance set 26 may include that the scroll wheel guide rail of 24 two sides of fixing seat is arranged in and is arranged in work
The two sides of dynamic seat 25 and the scroll wheel that cooperation is rolled with scroll wheel guide rail, pass through rolling of the scroll wheel on scroll wheel guide rail as a result,
It moves so that sliding seat 25 neatly executes expanding-contracting action in fixing seat 24.But the disclosure is not limited to above-mentioned two embodiment party
Formula, sliding seat guidance set 26 can use other reasonable arragement constructions, exist as long as can be realized reliable guiding sliding seat 25
Flexible function in fixing seat 24.
Optionally, as shown in Figure 33 and Figure 40, microscope carrier 2 includes the shape being fully retracted into sliding seat 25 relative to fixing seat 24
For limiting the sliding seat limit assembly of sliding seat 25 under state.That is, after sliding seat 25 is fully retracted in fixing seat 24, it should
Sliding seat limit assembly can play position-limiting action to sliding seat 25 so that sliding seat 25 and fixing seat 24 it is relatively fixed and in sample
Structure moves in microscope carrier transport establishment 1 as a whole during present treatment, for example, can by with above-mentioned movable trolley 122
Cooperation and moved in microscope carrier transport establishment 1 along microscope carrier moving direction Z1, thus in addition to execute pick and place operation when make sliding seat
Except 25 stretch relative to fixing seat 24, microscope carrier 2 sliding seat 25 and fixing seat 24 during the entire process of sample process are protected always
Hold relatively fixed, guarantee sample process operation normal operation.Here, optionally, sliding seat limit assembly is limit latch components,
For example, being can be set using latch components etc. usually used on drawer, this latch components in sliding seat 25 and be fixed
The bottom position of seat 24.Alternatively, alternatively, optionally, sliding seat limit assembly includes opposite two of magnetic force
Magnetic absorption member 27, two magnetic absorption members 27 are separately positioned on the opposing sidewalls of fixing seat 24 and sliding seat 25 on the first telescopic direction P
On.That is, two magnetic absorption members 27 are separately positioned on the most inboard inner wall along the first telescopic direction P of sliding seat 25 and fixing seat 24
On, wherein two magnetic absorption members 27 can use the permanent magnet of simple structure, be fully retracted in fixing seat 24 in sliding seat 25
When, two magnetic absorption members are mutually attached together and that sliding seat 25 and fixing seat 24 are reliably fixed is integral.
Optionally, container holding tank 251 is disposed in sliding seat 25 along the first compartment of terrain telescopic direction P multiple, thus
Multiple sample containers 3 in container holding tank 251 are being loaded by a microscope carrier 2 while being moved to corresponding workspace
It is synchronized in domain and executes corresponding sample process operation, improve whole sample process efficiency.Further optionally, each container holds
Receive and be provided with mistake proofing assembling structure 252 on slot 251, in a state of use can portion corresponding with the anti-misloading of sample container 3 match
It closes and sample container 3 is correctly installed in container holding tank 251 along nucleic acid extraction direction Z2.Here, mistake proofing assembling structure
252 can be according to the specific structure for the sample container 3 being put into container holding tank 251 come reasonable design, such as sample
The bottom of container 3 could be formed with the anti-misloading gap that portion is corresponded to as anti-misloading, in the case, mistake proofing assembling structure 252
Can be formed as the structures such as the convex rib being inserted correspondingly into anti-misloading gap, more specifically, for example, as shown in figure 41, it is convex
Playing muscle structure can accommodate along the first telescopic direction P across the opposite two sides and deviation container for being arranged in container holding tank 251
Slot 251 is along the side of nucleic acid extraction direction Z2, to adapt to the correspondence of sample container 3 in the case where preventing sample container 3
The arragement construction of holding tank, by above structure, when sample container 3 is put into the poor direction of container holding tank 251,
It cannot achieve depositing for sample container 3 since the anti-misloading portion of correspondence of mistake proofing assembling structure 252 and sample container 3 is not corresponding
It puts.
In order to enable the fit structure of fixing seat 24 and sliding seat 25 is simple and more rationalizes, optionally, such as Figure 39 and
Shown in Figure 40, the top of fixing seat 24 for open architecture and including fixing seat bottom plate 241 and surrounds 241 edge of fixing seat bottom plate
The fixing seat side wall 242 of arrangement, to be formed between fixing seat bottom plate 241 and fixing seat side wall 242 for accommodating sliding seat 25
Sliding seat accommodating chamber 243, fixing seat side wall 242 has opening so that sliding seat 25 is flexible in the side of the first telescopic direction P.
Optionally, as shown in Figure 41 and Figure 42, sliding seat 25 includes sliding seat pedestal 253 and is arranged at the top of sliding seat pedestal 253
Sliding seat top plate 254, sliding seat pedestal 253 and sliding seat top plate 254 cooperate and are formed with container holding tank 251, container
The opening of holding tank 251 is formed on sliding seat top plate 254.Here, guaranteeing the branch of sliding seat 25 while in order to save material
The intensity of sample container 3 is supportted, sliding seat pedestal 253 can be formed as rectangular plate body up and down, sliding seat top plate 254
It is covered on the top of sliding seat pedestal 253, here, sliding seat top plate 254 and sliding seat pedestal 253 can be fastened by bolt etc.
Part is connected as one, and in the case where sample container 3 is stored in microscope carrier 2, the part-structure of sample container 3 can be from activity
The opening of the container container 251 of seat top plate 254 is exposed, and another part structure is then located at sliding seat top plate 254 and sliding seat bottom
In the internal cavities that seat 253 is constituted, so that storage has the microscope carrier 2 of sample container 3 is whole to maintain suitable height, with
The movement being easy to implement in microscope carrier transport establishment 1.But it's not limited to that for the disclosure, can root for the specific structure of microscope carrier 2
It is reasonably arranged according to actual needs, as long as enabling to sliding seat 25 flexible relative to fixing seat 24.
Optionally, the top of fixing seat side wall 242 protrudes from the top surface of sliding seat top plate 254, and fixing seat side wall 242
Top is provided with towards sliding seat accommodating chamber 243 outstanding anti-detachment raised 244, this anti-detachment raised 244 with sliding seat top plate
There is gap between 254 top surface, prevent sample container 3 de- can cooperate in a state of use with sample container 3
From container holding tank 251.That is, after sliding seat 25 is put into sample container 3 relative to the stretching of fixing seat 24, in sliding seat
During 25 retract relative to fixing seat 24, the part that sample container 3 is exposed to sliding seat top plate 254 can be inserted into anticreep
It contacts from the gap between protrusion 244 and sliding seat top plate 254 and with anti-detachment raised 244 bottom surface, is thus moved in microscope carrier 2
Sample container 3 can be effectively prevent to be detached from from microscope carrier 2 in the process.
To sum up, the cooperation assembly of the microscope carrier including third embodiment as described above is by being assembled to microscope carrier for fixing seat 24
When in transport establishment 1, it can use sliding seat 25 and stretched relative to fixing seat 24 (in other words relative to microscope carrier transport establishment 1)
Contracting movement, so that the container holding tank 251 in sliding seat 25 exposes relative to microscope carrier transport establishment 1, in order to hold
The pick-and-place of row sample container 3 acts, and thus improves the pick-and-place efficiency and speed of sample container 3, and then improve the cooperation
The operating efficiency of assembly and sample processing device.
Above-mentioned first discloses microscope carrier and microscope carrier transport establishment with different structure feature into third embodiment,
This is it should be noted that may include as described above three in sample processing device and microscope carrier and the cooperation assembly of microscope carrier transport establishment
The microscope carrier of any one or more in kind embodiment and microscope carrier transport establishment, for example, first embodiment can be only included
It is provided with the microscope carrier of the first positioning seat in microscope carrier location structure and is provided with the microscope carrier transport establishment of the second positioning seat, or can be with
It is provided with the microscope carrier of first pulley component in microscope carrier guide frame including second embodiment and is provided with the load of microscope carrier guide rail
Platform transport establishment, or may include third embodiment including the fixing seat 24 that is mounted in microscope carrier transport establishment and be used for
Sample container 3 is accommodated and the microscope carrier of the sliding seat 25 flexible relative to fixing seat 24, to pass through work in microscope carrier transport establishment
Stretching or the retract action of dynamic seat 25 and convenient for picking and placing sample container 3.Or the cooperation assembly may include above-mentioned three
It any two or three in kind embodiment, using any two or three, is set respectively on same microscope carrier
Set corresponding structure, i.e., suitable for cooperate the microscope carrier assembly can include simultaneously the first positioning seat, first pulley component and
Any two or three in fixing seat and sliding seat.In the sample processing device of the disclosure, in order to understand and completely say
Structure, working principle and the processing method of the sample processing device of the bright disclosure, it includes such as that sample processing device, which uses,
The microscope carrier of the corresponding construction of upper three kinds of embodiments and microscope carrier transport establishment.
Secondly, total to the cooperation that can be suitable for referring to as described above referring to Fig. 1 to Figure 11 and Figure 22 to Figure 30
It is described in detail at structure, feature and the function and effect of the microscope carrier transport establishment with sample processing device.
The microscope carrier transport establishment 1 of the disclosure may include the multilayer microscope carrier shipping platform 11 along height direction H arrangement,
The working region for handling sample is disposed on each layer microscope carrier shipping platform 11, at least one of microscope carrier shipping platform 11
It is provided with microscope carrier driven in translation structure 12, the microscope carrier driven in translation structure 12 is for driving microscope carrier 2 flat in the transport of corresponding microscope carrier
Position is adjusted along microscope carrier moving direction Z1 in the working region of platform 11, at least one upper setting of remaining in microscope carrier shipping platform 11
There is microscope carrier to go up and down driving structure 13, microscope carrier lifting driving structure 13 is used to that microscope carrier 2 to be driven to go up and down along height direction H, so that
The switching position between each layer microscope carrier shipping platform 11 of microscope carrier 2.That is, being put down since microscope carrier transport establishment is provided with the transport of multilayer microscope carrier
Platform 11, each working region for handling sample rationally can hierarchically be arranged in multilayer microscope carrier shipping platform 11, at this time
Driving structure 13 can be gone up and down by microscope carrier drives microscope carrier 2 to go up and down between multilayer microscope carrier shipping platform 11 along height direction H
Switching freely, and can drive microscope carrier 2 on microscope carrier shipping platform 11 along the microscope carrier side of movement by microscope carrier driven in translation structure 12
It is mobile to Z1, so that microscope carrier 2 adjusts position neatly on multilayer microscope carrier shipping platform 11 to navigate to each workspace
On domain.Here, the microscope carrier transport establishment for being suitable for sample processing device can be disclosed microscope carrier transport establishment as described above,
That is, the microscope carrier transport establishment 1 includes the multilayer microscope carrier shipping platform 11 along height direction H arrangement, at this point, refer among the above the
One working region A, the second working region B and third working region C can be arranged in any in multilayer microscope carrier shipping platform 11
In one, microscope carrier 2 is moved on microscope carrier shipping platform 11 by microscope carrier driving mechanism, and microscope carrier driving mechanism includes that microscope carrier translation is driven
Dynamic structure 12 and microscope carrier go up and down driving structure 13, and at least one in microscope carrier shipping platform 11 is arranged in microscope carrier driven in translation structure 12
On person, and for driving microscope carrier 2 to adjust position along microscope carrier moving direction Z1 in the working region of corresponding microscope carrier shipping platform 11
Set, microscope carrier lifting driving structure 13 be arranged in microscope carrier shipping platform 11 remaining at least one on, and for driving 2 edge of microscope carrier
Height direction H lifting, so that the switching position between each layer microscope carrier shipping platform 11 of microscope carrier 2.From there through as described above
Microscope carrier transport establishment and sample processing device including the microscope carrier transport establishment enable to multiple working regions to realize three-dimensional row
Column, save the required arrangement areas of sample processing device, and make multiple working region characteristics of compact layout, improve microscope carrier driven in translation
Structure 12 and microscope carrier go up and down driving structure 13 for the drive efficiency of microscope carrier 2, and then can be improved sample process efficiency.
Here, microscope carrier driven in translation structure 12 as described above can be the specific implementation disclosed in above-mentioned cooperation assembly
Microscope carrier driven in translation structure 12 in mode omits its description herein to avoid repeating.
Optionally, as shown in figure 22, microscope carrier shipping platform 11 includes the upper layer microscope carrier fortune arranged along height direction H perforation
Defeated platform 111 and lower layer's microscope carrier shipping platform 112.As a result, microscope carrier transport establishment 1 can be realized the premise of three-dimensional arrangement
Under, weight is reduced by penetrating through the structure of the upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 that are arranged and is use up
The overall structure of microscope carrier transport establishment 1 may be simplified, meanwhile, have convenient for arrangement microscope carrier driven in translation structure 12 and microscope carrier liter
The effect of driving structure 13 is dropped, and is also convenient for driving microscope carrier 2 and is moved in microscope carrier transport establishment 1.Here, microscope carrier lifting driving knot
Structure 13 can be arranged in the perforation region of microscope carrier transport establishment 1, such as can be arranged under lower layer's microscope carrier shipping platform 112
The region Fang Guantong, it is dry in inoperative movement will not be generated to other movable parts such as microscope carrier 2, microscope carrier driven in translation structure 12
Relate to phenomenon.Wherein, microscope carrier guide rail 17 as described above is separately positioned on upper layer microscope carrier shipping platform 111 and the transport of lower layer's microscope carrier is flat
On platform 112, so that microscope carrier 2 moves on upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 along microscope carrier respectively
Direction Z1 is smoothly moved.Here, for the arrangement volume cost of microscope carrier driven in translation structure 12 and microscope carrier lifting driving structure 13
Open not limit this, condition needed for being handled according to actual sample is come reasonable design.For example, microscope carrier driven in translation knot
Structure 12 can be positioned only on upper layer microscope carrier shipping platform 111 or lower layer's microscope carrier shipping platform 112, or be arranged at upper layer load
On platform shipping platform 111 and lower layer's microscope carrier shipping platform 112, microscope carrier lifting driving structure 13 can be set transports in lower layer's microscope carrier
On the one or both ends position of platform 112.In addition, in order to steadily support upper layer microscope carrier shipping platform 111 and lower layer to carry
Platform shipping platform 112, and guarantee that microscope carrier 2 can work normally in each working region, it is transported by setting in lower layer's microscope carrier
The braced frame 19 of the lower part of platform 112 realizes secure support upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform
112。
It is optional in the case where microscope carrier shipping platform 11 as described above is suitable for microscope carrier processing equipment as described above again
Ground, as shown in Figures 4 to 6, the first working region A, the second working region B and third working region C are along microscope carrier moving direction
Z1 is sequentially arranged on upper layer microscope carrier shipping platform 111, and microscope carrier driven in translation structure 12 is arranged in upper layer microscope carrier shipping platform 111
On, microscope carrier goes up and down driving structure 13 and is arranged on lower layer's microscope carrier shipping platform 112, and the working region further includes more for storing
The initial storage area D of the microscope carrier of a microscope carrier 2, at least part region of the initial storage area D of microscope carrier are arranged in lower layer's microscope carrier fortune
On defeated platform 112.Here, a part of region of the initial storage area D of microscope carrier is arranged in for example as shown in Fig. 5, Fig. 6 and Figure 11
On lower layer's microscope carrier shipping platform 112, remainder region is arranged on upper layer microscope carrier shipping platform 111, herein for the ease of cloth
It sets microscope carrier 2 and microscope carrier 2 is left concentratedly together, the initial storage area D of microscope carrier is located at upper layer microscope carrier shipping platform 111 under
The position close to the first working region A of layer microscope carrier shipping platform 112.In addition, in the specific embodiment of the disclosure, microscope carrier
2 can be set there are six, wherein three microscope carriers 2 can be arranged in the initial storage area of microscope carrier of upper layer microscope carrier shipping platform 111
D, excess-three microscope carrier 2 can be arranged in the initial storage area D of microscope carrier of lower layer's microscope carrier shipping platform 112.But the disclosure is not
It is defined in this, for example, the initial storage area D of microscope carrier can be all arranged on lower layer's microscope carrier shipping platform 112.
Here, by arranging the first working region A, the second working region B and on upper layer microscope carrier shipping platform 111
Three working region C, therefore, the microscope carrier driven in translation structure 12 for driving microscope carrier 2 to move along microscope carrier moving direction Z1 can be only
Be arranged on upper layer microscope carrier shipping platform 111, herein for ease of control microscope carrier driven in translation structure 12 operation and make
The overall structure of microscope carrier transport establishment 1 and sample processing device is simplified, and microscope carrier driven in translation structure 12 can only be provided with one
It is a, i.e., all moving along microscope carrier moving direction Z1 of microscope carrier 2 are driven by a microscope carrier driven in translation structure 12.Here, can
Selection of land, microscope carrier driven in translation structure 12 are set as, and can drive microscope carrier 2 in the second working region B along in contrast to the microscope carrier side of movement
It is advanced to the direction of the separate third working region C of Z1 with step-by-step movement, with can be by mobile with the pipette tips of nucleic acid extraction mechanism 4
The sample in sample container 3 is extracted in the cooperation of the expanding-contracting action of the lifting action and magnetic bead transfer device 42 of device 41
Contained in nucleic acid and the nucleic acid of extraction is stored to each sample container 3.Here, in order to clearly illustrate microscope carrier driven in translation
Structure 12 drives the mode of microscope carrier 2 in the second working region B, on the basis of microscope carrier moving direction Z1, is located at microscope carrier movement side
It is defined as rear side to the upstream side Z1, is defined as front side, the first working region A and third work positioned at the downstream side microscope carrier moving direction Z1
Make rear side and front side that region C is located at the second working region B, in the case, contains stage translation in the second working region B
Driving structure 12 drives microscope carrier 2 to carry out step-by-step movement backward movement towards the rear side in contrast to microscope carrier moving direction Z1, from there through
With the pipette tips mobile device 41 of nucleic acid extraction mechanism 4 and the cooperation of magnetic bead transfer device 42, the nucleic acid of extraction is stored to sample
In the sample holding tank of 3 front side of container.The microscope carrier 2 of nucleic acid extraction will be completed from the by microscope carrier driven in translation structure 12 later
Two working region B are moved to third working region C along microscope carrier moving direction Z1, at this point, since the nucleic acid extracted is stored in
In the sample holding tank of 3 forward position of sample container, so that second mechanical arm is fast by the way of smaller movement routine
Speed and easily dispense the nucleic acid into multiple deep-well plates 100, further improve sample process operating efficiency.But the disclosure
It's not limited to that, and microscope carrier driven in translation structure 12 may be set to be, and can drive microscope carrier 2 in the second working region B along load
Platform moving direction Z1 is advanced with step-by-step movement, in the case, cell pyrolysis liquid holding tank, protein digestibility enzyme solutions holding tank,
Cleaning solution holding tank, eluent holding tank and nucleic acid holding tank are on sample container along the side in contrast to microscope carrier moving direction Z1
To being sequentially arranged, the nucleic acid extracted is then placed in the nucleic acid holding tank positioned at rear side of sample container 3.
When executing sample process operation, microscope carrier 2 can be driven in upper layer microscope carrier by microscope carrier driven in translation structure 12
The second working region B and third working region C are successively moved to from the first working region A on shipping platform 111 and execute sample
Operation is handled, here, not limiting this for microscope carrier driven in translation structure 12 disclosure such as the driving sequences of each microscope carrier 2
It is fixed, if can and reasonably driving each microscope carrier 2 respective accordingly completion sample process operation effectiveness.For example,
In order to save the whole microscope carrier processing required time, microscope carrier driven in translation structure 12 can use and alternately drive each microscope carrier 2
It is moved to the mode of corresponding working region, this is described in detail subsequent.In addition, in upper layer microscope carrier shipping platform
The microscope carrier 2 that entire sample process operation is completed on 111 can be moved to microscope carrier by the lifting that microscope carrier goes up and down driving structure 13
Initial storage area D can also be placed directly into lower layer's microscope carrier for example, the original initial placement position of microscope carrier 2 can be moved to
On shipping platform 112, this disclosure is not particularly limited.By structure as described above, sample processing device can be
Most sample process operation is executed on upper layer microscope carrier shipping platform 111, lower layer's microscope carrier shipping platform 112 is mainly used to arrange
The initial storage area D of microscope carrier for storing microscope carrier 2, so that upper layer microscope carrier shipping platform 111 He of the microscope carrier 2 in three-dimensional arrangement
Respectively divide the work on lower layer's microscope carrier shipping platform 112 and clearly execute corresponding operation, and then effectively improves sample process operating efficiency.
Optionally, as shown in figure 11, microscope carrier driven in translation structure 12 is arranged in the side of upper layer microscope carrier shipping platform 111
On wall, and including the first driving motor 121, the first transmission component being connected with the transmission of the output shaft of first driving motor 121,
And the movable trolley 122 that connect and can separatably cooperate with microscope carrier 2 with the first transmission component, the first transmission component are used for
The rotary motion of first driving motor 121 is converted to the linear movement of movable trolley 122, to enable to movable trolley 122
Working region locating for microscope carrier 2 is adjusted by the cooperation with microscope carrier 2.Here, can be adopted for microscope carrier driven in translation structure 12
With the arragement construction of various reasonable, for example, microscope carrier driven in translation structure 12 can be the microscope carrier that refers in cooperation assembly as above
Driven in translation structure, therefore there is construction identical with the microscope carrier driven in translation structure in above-mentioned cooperation assembly, feature and work
With effect, for example, optionally, the first transmission component is that V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair pass
Dynamic matching mechanism, optionally, the first transmission component are V belt translation matching mechanism, and including the first transmission belt 123, setting first
123 side of transmission belt and with the first driving wheel 124 of the output axis connection of the first driving motor 121 and setting first transmission
The first driven wheel 125 with 123 other sides, movable trolley 122 are mounted on the first transmission belt 123.These technical characteristics, correlation
Explain, expansion scheme and generated technical effect refer in the related content part of above-mentioned cooperation assembly, herein for
It avoids repeating to repeat no more.
Optionally, as shown in Figure 25 to Figure 30, the downside of the first transmission belt 123 is located in upper layer microscope carrier shipping platform 111
The trolley guide rail 126 extended along microscope carrier moving direction Z1 is provided in inner wall section, movable trolley 122 is fixed on the first transmission belt
In 123 downside band part, the bottom of movable trolley 122 is provided with the second pulley slideably cooperated with trolley guide rail 126
Component 127.Optionally, the bottom of movable trolley 122 is arranged in by second pulley mounting base 128 for second pulley component 127, the
Two pulley mounting bases 128 are located at 123 downside of the first transmission belt and are supported on trolley guide rail 126 by second pulley component 127.
Here, can be using the first pulley component 23 referred in microscope carrier guide frame as described above for second pulley component 127
Structure, specifically can be with reference to the structure of first pulley component 23 shown in Figure 35 to Figure 38, second pulley in the case
The relevant technologies feature, explanation, expansion scheme and generated technical effect of component 127 etc. can be oriented to above-mentioned microscope carrier and tie
The relevant technologies feature of the first pulley component 23 referred in structure, explanation, expansion scheme and generated technical effect are similar
Seemingly, herein in order to avoid its description is omitted in repetition.
Optionally, as shown in figures 30 and 31, it is telescopically provided with stretching structure 8 on movable trolley 122, microscope carrier 2 is right
It should be on the side side wall of movable trolley 122, being provided with for cooperating and the locking structure 21 that is mutually locked with stretching structure 8.
Movable trolley 122 can successively drive multiple microscope carriers 2 to move by way of the cooperation and disengaging of stretching structure 8 and locking structure 21
It moves to corresponding working region, so that microscope carrier 2 is consecutively carried out sample process operation.Optionally, stretching structure 8 wraps
The second magnet plunger 81 is included, the second magnet plunger 81 has lockup state and unlocked state, and in lockup state, the second magnet plunger 81 is disconnected
Electricity, so that the push rod of the second magnet plunger 81 is stretched out and snapped on locking structure 21 and locking microscope carrier 2 and movable trolley 122；
In unlocked state, the second magnet plunger 81 is powered, unlock pusher retracted by the magnetic force generated in the second magnet plunger 81
Microscope carrier 2 and movable trolley 122, locking structure 21 are formed to have lock groove or locking with the shape of the second magnet plunger 81 cooperation
Hole.The relevant technologies feature of stretching structure 8 and locking structure 21, explanation, expansion scheme and generated technology are imitated
Fruit etc. refers in the relevant technologies partial content in above-mentioned microscope carrier and the cooperation assembly of microscope carrier transport establishment, herein in order to
It avoids repeating to repeat no more.
Optionally, as shown in Figure 22 to Figure 27, Figure 32 and Figure 33, microscope carrier transport establishment includes being arranged in microscope carrier transporter
On the side inner sidewall of structure 1 and along the second positioning seat 15 that microscope carrier moving direction Z1 extends, with each for navigating to microscope carrier 2
Working region has been positioned apart from multiple second positioning regions, the second positioning region along microscope carrier moving direction Z1 on the second positioning seat 15
The first positioning region being arranged on the side side wall with microscope carrier 2 cooperates or separates, in lockup state, all second positioning
Portion is with the first position portion from so that microscope carrier 2 is in the state of with movable 122 locking of trolley in upper layer microscope carrier shipping platform
It is moved freely on 111, in unlocked state, the first positioning region is located at opposite with any one second positioning region in the second positioning region
When position, the first positioning region cooperates with corresponding second positioning region, so that microscope carrier 2 is in the state unlocked with movable trolley 122
Under navigate on upper layer microscope carrier shipping platform 111.As described above, upside microscope carrier shipping platform of the microscope carrier 2 in microscope carrier transport establishment 1
On 111 along microscope carrier moving direction Z1 moving process, when the first positioning region is placed exactly in and in microscope carrier transport establishment 1 multiple second
When an opposite position in the second positioning region in positioning region, the first positioning region and the second opposite positioning region cooperation and by microscope carrier
2 navigate to the corresponding working region of microscope carrier transport establishment 1 (such as the first working region A, the second working region B or third work
Region C etc.), the reliability of positioning of microscope carrier 2 is effectively ensured, thereby executing corresponding sample process operation (such as first work
Region A executes sample and dispenses operation, executes nucleic acid extraction operation in the second working region B, executes nucleic acid in third working region C
Detect operation), the first positioning region separates with the second opposite positioning region and microscope carrier 2 is transported relative in microscope carrier later
Mechanism 1 is in and moves freely state, (such as moves from the initial storage area D of microscope carrier so that microscope carrier 2 is moved to subsequent work region again
It moves and is moved to the second working region B to the first working region A, from the first working region A, be moved to from the second working region B
Three working region C or be moved to initial storage area D of microscope carrier etc. from third working region C), and pass through as described above the
The operation repeatedly of cooperation and separation between one positioning region and the second positioning region, and then favorably accomplish sample process operation.
Optionally, the second positioning region is the first magnet plunger 151, and the first positioning region is locating slot 221, the first magnet plunger 151
With working condition and off working state, in working condition, the first magnet plunger 151 power-off, so that the first magnet plunger 151 pushes away
Bar is stretched out and is snapped onto locating slot 221, and in off working state, the first magnet plunger 151 is powered, to pass through the first magnet plunger 151
The magnetic force of interior generation makes pusher retracted and is detached from locating slot 221.Optionally, is provided on the side side wall of microscope carrier 2
One positioning seat 22, locating slot 221 are formed on the first positioning seat 22, and the second positioning seat 15 is adjacent to each first magnet plunger 151
On position, it is separately provided for the position sensor of the position of the first positioning seat 22 of detection, in multiple position sensors
Position sensor when detecting the position of first positioning seat 22 so that corresponding in the first magnet plunger 151 described
The first magnet plunger and locating slot 221 of one position sensor cooperate.Optionally, position sensor is groove profile optoelectronic position sensing
Device 16, the slot of groove profile photoelectrical position sensor 16 towards upper layer microscope carrier shipping platform 111 inside opening, on the first positioning seat 22
It is formed with the block 222 of the slot for passing through groove profile photoelectrical position sensor 16.
It the relevant technologies feature of first positioning region and the second positioning region as described above, explanation, expansion scheme and is produced
Raw technical effect etc. refers in the relevant technologies partial content of above-mentioned microscope carrier location structure, herein in order to avoid repeating not
It repeats again.
Optionally, referring to figs. 2 to Fig. 4, Fig. 9 and Figure 11, it includes being arranged in lower layer's microscope carrier that microscope carrier, which goes up and down driving structure 13,
Lifting fixed pedestal 131 on shipping platform 112 and liftably it is arranged on lifting fixed pedestal 131 and can be with microscope carrier 2
The lift cylinders 132 of cooperation can be detached from so that microscope carrier 2 upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 it
Between switching position.Wherein, the bottom of the microscope carrier 2 is provided with for cooperating with lift cylinders 132 can be realized the lifting of lifting
Cylinder mating groove, the movable end of lift cylinders 132 are provided with the support base for being used to support microscope carrier, are provided on the support base for being inserted into
To the locking projections in the lift cylinders mating groove, enabled to from there through the cooperation of lift cylinders mating groove and locking projections
Microscope carrier 2 steadily realizes lifting.Here, one or two has can be set in microscope carrier lifting driving structure 13.For example, being provided with
In the case where two microscope carrier lifting driving structures 13, it is flat that microscope carrier lifting driving structure 13 can be separately positioned on the transport of lower layer's microscope carrier
On position in platform 112 below the first working region A and third working region C, lower layer's microscope carrier shipping platform 112 as a result,
The microscope carrier 2 of the initial storage area D of microscope carrier can be moved to upper layer by the lift cylinders 132 that microscope carrier goes up and down driving structure 13
On microscope carrier shipping platform 111, and the microscope carrier 2 that detection nucleic acid is completed in the C of third working region can pass through other microscope carrier liter
The lift cylinders 132 of drop driving structure 13 are moved on lower layer's microscope carrier shipping platform 112, at this point, lower layer's microscope carrier shipping platform 112
On the structure of microscope carrier driven in translation structure 12 as described above etc. can be set, to be moved into lower layer's microscope carrier for driving
Microscope carrier 2 on shipping platform 112 is moved to the initial storage area D of microscope carrier.
For another example, in the case where microscope carrier lifting driving structure 13 is provided with one, optionally, as shown in figure 11, microscope carrier fortune
Microscope carrier return driving structure 18 is provided on 1 side wall of transfer mechanism, the microscope carrier return driving structure 18 and lifting fixed pedestal 131 connect
It connects, and for driving direction of the microscope carrier lifting driving structure 13 along microscope carrier moving direction Z1 or in contrast to microscope carrier moving direction Z1 to move
It is dynamic.The cooperation of driving structure 13 need to be only gone up and down by microscope carrier return driving structure 18 and a microscope carrier as a result, that is, microscope carrier return
Driving structure 18 drives the microscope carrier lifting driving structure 13 for being mounted with microscope carrier 2 mobile, so that microscope carrier 2 is transported in lower layer's microscope carrier
Position is adjusted on platform 112 to the initial storage area D of microscope carrier, so that microscope carrier transport establishment overall structure is simpler and closes
It is physical and chemical.Here, here, microscope carrier return driving structure 18 can use the arrangement of various reasonable, for example, microscope carrier return drives
Structure 18 can be can be along structures such as the microscope carrier moving direction Z1 telescoping cylinders to stretch, alternatively, microscope carrier return structure 18 can be
Feed screw nut fit structure etc., in the case, nut are fixed on the lifting fixed pedestal 131 of microscope carrier lifting driving structure 13
On, screw rod can extend to the other end from one end of lower layer's microscope carrier shipping platform 112, and thus, it is possible to pass through nut on lead screw
It slides to drive the whole direction along microscope carrier moving direction Z1 or in contrast to microscope carrier moving direction Z1 of microscope carrier lifting driving structure 13
It is mobile.
But the microscope carrier return driving structure 18 of the disclosure is not limited to the above embodiment, for example, optionally, microscope carrier returns
Position driving structure 18 includes the second driving motor 181 and the second biography being connected with the transmission of the output shaft of second driving motor 181
Dynamic component, lifting fixed pedestal 131 are mounted on the movable part of the second transmission component, and the second transmission component is used for the second driving
The rotary motion of motor 181 is converted to microscope carrier lifting driving structure 13 along the linear movement of microscope carrier moving direction Z1, or on the contrary
Linear movement in the direction of microscope carrier moving direction Z1.So that microscope carrier lifting driving structure 13 drives microscope carrier 2 to carry from upper layer
It is detached from and is moved on lower layer's microscope carrier shipping platform 112 on platform shipping platform 111, and will be carried by microscope carrier return driving structure 18
Platform 2 is moved to the initial storage area D of microscope carrier, or is detached from from lower layer's microscope carrier shipping platform 112 and is moved to upper layer microscope carrier fortune
The initial storage area D of the microscope carrier of defeated platform 111.Here, the second transmission component can use the structure of various reasonable, such as optional
Ground, the second transmission component be V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission matching mechanism, thus, it is possible to
Enough improve reliable transmission and transmission efficiency.By structure as described above, that is, starts the second driving motor 181 and output it
The rotary force of power axis is converted to the linear movement that microscope carrier goes up and down driving structure 13 by the transmitting of the first transmission component, guarantees to carry
Platform goes up and down reliable mobile of the driving structure 13 on lower layer's microscope carrier shipping platform 112, and then ensure that microscope carrier 2 is transported in lower layer's microscope carrier
The transportation of defeated platform 112.
Optionally, the second transmission component can be formed as and the first transmission group in microscope carrier driven in translation structure as described above
The identical structure of part, specifically optionally, as shown in Figure 25 and Figure 27, the second transmission component is V belt translation matching mechanism, and including
With lifting fixed pedestal 131 connect the second transmission belt 182, be arranged 182 side of the second transmission belt and with the second driving motor
Second driving wheel 183 of 181 output axis connection, the second driven wheel 184 that 182 other side of the second transmission belt is set, lifting
Fixed pedestal 131 is fixed on the second transmission belt 182, wherein V belt translation matching mechanism has stable drive, buffering absorbing, knot
The advantages that structure is simple, at low cost, working service facilitates only passes through simple knot as described above on lower layer's microscope carrier shipping platform 112
The V belt translation matching mechanism of structure allows for and the second transmission belt 182 is reliable and easily drive microscope carrier goes up and down driving structure 13
It is realized in moving range flexibly mobile.
Here, optionally, the second transmission component is set in order to which more smoothly and reliably supporting platform goes up and down driving structure 13
It is equipped with two groups and is separately positioned on the two sides inner wall of lower layer's microscope carrier shipping platform 112, two groups of the second driven wheel 184 passes through biography
Moving axis 185 mutually interlocks, wherein in order to rise during the second transmission belt 182 drives microscope carrier lifting driving structure 13 mobile
It is remained to guiding microscope carrier lifting driving structure 13 and does linear movement, optionally, as shown in figure 9, lower layer's microscope carrier shipping platform
Be located on the position of the lower section of the second transmission belt 182 in 112 two sides inner wall, be respectively arranged with extend along microscope carrier moving direction Z1 and
With the lifting fixed pedestal guide rail 133 of the two sidewalls cooperation of lifting fixed pedestal 131.Wherein, by the load of structure as described above
Platform goes up and down driving structure 13 suitable for driving microscope carrier 2 going up and down so that going up and down fixed pedestal 131 in the case where sample processing device
It moves on fixed pedestal guide rail 133 and microscope carrier 2 is allow to return back to the initial storage area D of microscope carrier.Microscope carrier goes up and down driving structure 13
That first arranged on microscope carrier bottom in microscope carrier guide frame as described above can be set is sliding the bottom of lifting fixed pedestal 131
The mutually isostructural third pulley assembly of second pulley component 127 of wheel assembly 23 and the bottom arrangement of movable trolley 122.Microscope carrier liter
Drop driving structure 13 is rollably supported on lifting fixed pedestal guide rail 133 by third pulley assembly, to go up and down fixation
Base rail 133 can be smoothly directing to microscope carrier lifting driving structure 13 along microscope carrier moving direction Z1 or in contrast to the microscope carrier side of movement
To the linear movement in the direction of Z1, simultaneously, additionally it is possible to be matched by third pulley assembly and the rolling of lifting fixed pedestal guide rail 133
It closes and movement that is more flexible and easily realizing microscope carrier lifting driving structure 13.
In addition, in the sample processing device of the disclosure, optionally, as in figs. 3 and 11, the initial storage area D of microscope carrier
It is arranged in the side of upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112 and is located at close to the first workspace
The region of domain A, microscope carrier transport establishment 1 corresponding to the initial storage area D of microscope carrier side wall on be formed be used for so that microscope carrier 2 stretch out
Or the microscope carrier retracted picks and places opening 14, microscope carrier 2 is set as, and picking and placing opening 14 by microscope carrier in the initial storage area D of microscope carrier can
It stretches out or retracts relative to microscope carrier transport establishment 1.Here, microscope carrier 2 is configured to fixing seat 24 as described above and scalable
The structure of the sliding seat 25 in fixing seat 24 is arranged in ground, as a result, in the case where microscope carrier 2 is located at the initial storage area D of microscope carrier,
When needing to pick and place the sample container 3 on microscope carrier 2,24 are done relative to regulation by sliding seat 25 and is stretched along the first telescopic direction P
Contracting and opening 14 can be picked and placed from microscope carrier expose or be hidden into microscope carrier and pick and place in opening 14, thus improve sample container 3 and take
Efficiency and speed are put, that improves sample processing device recycles efficiency.
Optionally, as shown in Figures 4 to 7, working region further includes first of the sample dispensing for the first working region A
Consumptive material storage area E, the first lifting arm 6 are arranged in the first consumptive material storage area E, and first consumptive material storage area E, which has, to be used
In the sampling hammerhead storage area E1 of storage sampling hammerhead 200 and for storing the probe tube for accommodating the probe tube 300 of sample
Storage area E2, the first consumptive material storage area E are arranged on upper layer microscope carrier shipping platform 111 and are located at the initial storage area of microscope carrier
The top of D, first mechanical arm 6, which is arranged, in the first consumptive material storage area E and to be arranged as, can be in the first working region A and the
It is moved back and forth between one consumptive material storage area E, with the sample container 3 that can be mounted in on the first working region A on microscope carrier 2
Sample in interior dispensing probe tube 300.As a result, each working region arrangement in microscope carrier transport establishment 1 is more rationalized,
By the way that the first consumptive material storage area E to be disposed adjacent to the top position of the first working region A, first mechanical arm 6 loads sampling
After sampling hammerhead 200 in the E1 of pipette tips storage area, it is moved in the E2 of probe tube storage area and draws in probe tube 300
Sample, be moved to the first working region A later and on microscope carrier injecting sample in each sample container 3, it is thus more fast
Speed and the operation for being conveniently carried out dispensing sample, and sample process easy to automate operates.
Optionally, as shown in fig. 7, working region further includes the dispensing detection reagent and extraction for third working region C
Nucleic acid the second consumptive material storage area F, the second lifting arm 7 be arranged in the second consumptive material storage area F, second consumptive material storage
Region F has the dispensing pipette tips storage area F1 for being used to store dispensing pipette tips 400, deposits for storing the deep-well plates of deep-well plates 100
Put region F2 and for storing the detection reagent receiving pipe storage area for accommodating the detection reagent receiving pipe 500 of detection reagent
F3, the second consumptive material storage area F are arranged in the other side of upper layer microscope carrier shipping platform 111 and are located at close to third working region C
Region, second mechanical arm 7 can move back and forth between third working region C and the second consumptive material storage area F, with can be to
Dispensed respectively in multiple deep-well plates 100 in second consumptive material storage area F detection reagent accommodate pipe 500 in detection reagent and,
The nucleic acid being mounted in the C of third working region in the sample container 3 on microscope carrier 2, to detect nucleic acid.Pass through as a result,
Second consumptive material storage area F is disposed adjacent to the position of third working region C, second mechanical arm 7 is moved to dispensing pipette tips and deposits
Put region F1 and load dispensing pipette tips 400 after, be moved to detection reagent accommodate pipe storage area F3 and draw detection reagent appearance
The detection reagent received in pipe 500 is moved to deep-well plates storage area F2 later and injects detection reagent into deep-well plates 100, it
After second mechanical arm 7 continues the dispensing pipette tips 400 for reloading new afterwards, moves third working region C and draw each on microscope carrier
Nucleic acid in sample container 3 is moved to deep-well plates storage area F2 later and injects nucleic acid into deep-well plates 100, thus comes
The operation of dispensing detection reagent and nucleic acid is more quickly and easily executed, and sample process easy to automate operates.
To sum up, each structure, that is, nucleic acid extraction mechanism of sample processing device, pipette tips ejection releasing mechanism, microscope carrier are transported
The structures such as mechanism and microscope carrier and the cooperation assembly of the two, microscope carrier location structure, microscope carrier guiding mechanism are described in detail.Herein
It should be noted that the relevant technologies feature etc. in each structure can be combined in any combination, it is this to combine
To technical solution obviously also belong to the protection scope of the disclosure.For example, microscope carrier positioning knot can be set on the same microscope carrier 2
The drawer that the first pulley component 23 in the first positioning region, microscope carrier guide frame, fixing seat 24 and sliding seat 25 in structure cooperate
Various features in formula construction, for another example, being provided with as described above can in the microscope carrier transport establishment 1 of multilayer microscope carrier shipping platform 11
Be provided in microscope carrier location structure as described above the second positioning region, the microscope carrier guide rail 17 in microscope carrier guide frame, microscope carrier
Driven in translation structure 12, microscope carrier lifting driving structure 13, microscope carrier pick and place various features in opening 14 etc..Those skilled in the art
It is to be understood that combination as described above is fallen in the protection scope of the disclosure.
Based on the structure of sample processing device as described above, below to the sample process using above-mentioned sample processing device
Method is illustrated.Here, the sample processing method in order to more fully, more clearly illustrate the disclosure, following middle samples
The sample processing device that processing method is related to include all the relevant technologies features as described above preferred embodiment
Construction is to be described in detail.But this only makes as more comprehensively, clearly explaining the technical solution of the disclosure
, it is not used as the purposes of restriction but disclosure protection scope.
Referring to figs. 1 to Figure 44, the disclosure provides a kind of following sample process using sample processing device as described above
Method.The sample processing method includes: first step S1, and microscope carrier 2 is moved to the first working region along microscope carrier moving direction Z1
A, using dispensing sample in first mechanical arm 6 respectively each sample container 3 on microscope carrier 2；Second step S2, microscope carrier 2 from
First working region A is moved to the second working region B along microscope carrier moving direction Z1, is extracted on microscope carrier 2 using nucleic acid extraction mechanism 4
Sample container 3 in sample contained in nucleic acid, and the nucleic acid of extraction is stored to each sample container 3；Third step
Rapid S3, microscope carrier 2 are moved to third working region C from the second working region B, have detection reagent to dispensing using second mechanical arm 7
Multiple deep-well plates 100 in dispensing microscope carrier 2 on sample container 3 in store nucleic acid and detect nucleic acid, from first work
On the microscope carrier moving direction Z1 of region A to third working region C, the microscope carrier 2 in adjacent every two microscope carrier 2 positioned at front side is being held
While any one step into third step S3 of row first step S1, any one step is executed positioned at the microscope carrier 2 of rear side
Rapid previous step.
Here, executing sample processing method as described above using the sample processing device of structure as described above.Such as
Motion mode of the microscope carrier 2 in microscope carrier transport establishment 1 in the upper sample processing device can be using the master voluntarily controlled
Dynamic driving method, can also be using the passive matrix mode passively controlled by other structures, as long as enabling to microscope carrier 2 mobile
Corresponding work is executed to each working region.Sample processing method above disclosured is used in microscope carrier
Transport establishment 1 moves up the movable sample process mode of microscope carrier of dynamic load platform 2, and not uses in existing and usually pass through moving machine
The mode of tool arm executes the fixed sample process mode of microscope carrier of respective sample processing operation to microscope carrier.Therefore, the disclosure is adopted
With the sample under the sample processing method and the existing middle stationary state using microscope carrier of movable microscope carrier by mobile machine arm mode
Processing method is compared, and multiple microscope carriers 2 of the disclosure can be performed simultaneously corresponding operation in corresponding working region.Specifically
Ground, for example, being located at first being located on the foremost side first microscope carrier 2 on microscope carrier moving direction Z1 when executing third step S3
Second microscope carrier 2 of the next side of a microscope carrier 2 executes second step S2, meanwhile, the third positioned at second next side of microscope carrier 2 carries
Platform 2 executes first step S1, synchronously executes in corresponding working region in multiple microscope carriers 2 of same period as a result,
Respective operation so as to the activity duration of processing needed for significantly shortening entire sample, and then significantly improves at sample
Manage efficiency.
Optionally, as described in Figure 7, working region further includes the second consumptive material storage area F, second consumptive material storage area F
With the dispensing pipette tips storage area F1 for storing dispensing pipette tips 400, the deep-well plates storage area for storing deep-well plates 100
F2 and for store accommodate detection reagent detection reagent accommodate pipe 500 detection reagent accommodate pipe storage area F3, second
Consumptive material storage area F is arranged in the region of the close third working region C of microscope carrier transport establishment 1, and the setting of second mechanical arm 7 is the
It in two consumptive material storage area F and is arranged as, can be moved back and forth between third working region C and the second consumptive material storage area F,
The detection in detection reagent container 500 can be dispensed respectively in multiple deep-well plates 100 into the second consumptive material storage area F
Reagent and, the nucleic acid being mounted in the C of third working region in the sample container 3 on microscope carrier 2, in third step S3,
Before second mechanical arm 7 dispenses the nucleic acid dispensing step S32 of nucleic acid into the deep-well plates 100 of the second consumptive material storage area F, also
Step S31 is dispensed including detection reagent, is divided using second mechanical arm 7 into multiple deep-well plates 100 of the second consumptive material storage area F
Detection reagent is infused, while starting to execute first step S1, the synchronous detection reagent that executes dispenses step S31.That is, making first
Detection reagent dispensing step S31 in step S1 and third step S3 is synchronously executed, thus upper in microscope carrier moving direction Z1
Detection examination when first microscope carrier 2 of most front side is sequentially completed first step S1 and second step S2, in third step S3
Agent dispensing step S31 also terminates or terminates in advance just, so that first microscope carrier 2, which eliminates, waits detection reagent dispensing
The nucleic acid that step S31 completes the required time and can directly execute third step S3 dispenses operation, to save considerably sample
The time required to present treatment, sample process efficiency is further improved.Specifically, in the detection reagent dispensing for executing third step S3
When step S31, second mechanical arm 7 be moved to dispensing pipette tips storage area F1 and load dispensing pipette tips 400 after, be moved to detection
Reagent accommodates pipe storage area F3 and draws the detection reagent in detection reagent receiving pipe 500, is moved to deep-well plates storage later
Region F2 and detection reagent is injected into deep-well plates 100, second mechanical arm 7 continues the dispensing pipette tips 400 for reloading new later
Afterwards, it moves third working region C and draws the nucleic acid that extraction is completed on microscope carrier in each sample container 3, be moved to later
Deep-well plates storage area F2 and nucleic acid is injected into deep-well plates 100, thus come more quickly and easily execute dispensing detection examination
The operation of agent and nucleic acid, and sample process easy to automate operates.
Optionally, in first step S1, the sample in probe tube 300 is drawn after the loading sampling hammerhead 200 of first mechanical arm 6
Originally sample is dispensed in each sample container 3 on microscope carrier 2, the sampling hammerhead 200 that would be used for dispensing sample later is set
In in sample container 3.That is, the sampling hammerhead 200 for being used in dispensing sample in first step S1 can be placed into sample appearance
It receives in device 3 and can be re-used in second step S2, in other words, the sampling gun for being used to dispense sample in first step S1
Head can share a pipette tips for extracting the sampling hammerhead of nucleic acid in second step S2, therefore save on sampling hammerhead, save
Consumptive material resource, and then save sample process cost.
Further optionally, in first step S1, in first mechanical arm 6 in each sample container 3 on microscope carrier 2
It further include internal standard dispensing step S11 before the sample dispensing step S12 for dispensing sample, first mechanical arm 6 loads sampling hammerhead
Internal standard is drawn after 200 and dispenses internal standard in each sample container 3 on microscope carrier 2, be would be used for dispensing interior target later and is taken
Sample pipette tips 200 are discarded.Specifically, in internal standard dispensing step S11, first mechanical arm 6 draws internal standard after loading sampling hammerhead,
And internal standard is dispensed in the cell pyrolysis liquid holding tank of each sample container 3 on the microscope carrier 2 into the first working region A, it
Target sampling hammerhead 200 in dispensing will be used in afterwards to discard.Internal standard as described above is using whole noncompetitive internal standard, thus
It can monitor the overall process of nucleic acid extraction and augmentation detection, while avoid false negative.Here, accommodating interior target internal standard
Accommodating pipe can be individually positioned on the corresponding position of each microscope carrier, such as can be formed on the sliding seat top plate 254 of microscope carrier 2
There is the internal standard holding tank 29 that pipe is accommodated for accommodating internal standard, in order to interior target dispensing.But it's not limited to that for the disclosure, described
Internal standard accommodates pipe and can also be disposed adjacent on the position of the first working region, for example, as described above first can be arranged in
On the E of consumptive material storage area.
Optionally, as shown in Fig. 5, Fig. 6 and Figure 12, nucleic acid extraction mechanism 4 includes: pipette tips mobile device 41, which moves
Dynamic device 41 can be arranged in up and down in microscope carrier transport establishment 1 along height direction H, pipette tips are separatably arranged in for driving
Sampling hammerhead 200 in mobile device 41 enables to sampling hammerhead 200 to adsorb sample and holds along height direction H shift position
The liquid in liquid or discharge sampling hammerhead 200 received in device 3；Magnetic bead transfer device 42, the magnetic bead transfer device 42 include energy
The magnetic part in microscope carrier transport establishment 1 is telescopically arranged in enough directions along close to or far from sampling hammerhead 200, to enable to
Magnetic part, which has, provides acting in sampling hammerhead 200 for magnetic state and removing for magnetic force to the magnetic bead in sampling hammerhead 200
The demagnetizing state of the magnetic force of magnetic bead, microscope carrier driving mechanism includes microscope carrier driven in translation structure 12, for driving microscope carrier 2 corresponding
Position is adjusted along microscope carrier moving direction Z1 in the working region of microscope carrier shipping platform 11, and microscope carrier driven in translation structure 12 is arranged
For that can drive microscope carrier 2 in the second working region B along as remote in contrast to microscope carrier moving direction Z1 in second step S2
Direction from third working region C is advanced with step-by-step movement, can pass through the lifting action and magnetic bead with pipette tips mobile device 41
The cooperation of the expanding-contracting action of transfer device 42 is sequentially inserted into each holding tank of sample container 3 and extracts sample receiving
Nucleic acid contained in sample in device 3, and the nucleic acid of extraction is stored to each sample container 3.
Containing stage translation driving structure 12 in the second working region B as a result, drives microscope carrier 2 towards in contrast to microscope carrier movement side
Step-by-step movement backward movement is carried out to the rear side of Z1, is turned from there through with the pipette tips mobile device 41 and magnetic bead of nucleic acid extraction mechanism 4
The cooperation of moving device 42 stores the nucleic acid of extraction to the nucleic acid holding tank of 3 front side of sample container.It is flat by microscope carrier later
It moves driving structure 12 and the microscope carrier 2 for completing nucleic acid extraction is moved to third work along microscope carrier moving direction Z1 from the second working region B
Make region C, at this point, being stored in due to the nucleic acid extracted in the nucleic acid holding tank of 3 forward position of sample container, makes
It obtains second mechanical arm 7 and quickly and easily dispenses the nucleic acid into multiple deep-well plates 100 by the way of smaller movement routine,
Further improve sample process operating efficiency.
In addition, the microscope carrier driven in translation structure 12, the pipette tips mobile device 41 that are used in sample processing method as described above
With magnetic bead transfer device 42 can using the microscope carrier driven in translation structure 12 referred in microscope carrier transport establishment as described above and
The pipette tips mobile device 41 referred in nucleic acid extraction mechanism 4 construction identical with the structure of magnetic bead transfer device 42.Herein in order to
It avoids repeating to omit its description.
Further optionally, as shown in figure 43, second step S2 includes: that sampling hammerhead loads step, pipette tips mobile device 41
Load sampling hammerhead 200；Cleavage step S21, sampling hammerhead 200 draw sample and contain the thin of magnetic bead into sample container 3
The sample is added in cellular lysate liquid, so that obtaining the bead complexes that adsorption has nucleic acid, sampling hammerhead 200 after sample cracking
Draw the solution containing bead complexes, magnetic part close to sampling hammerhead direction stretch out for magnetic state under, sampling hammerhead
200 solution to spue in addition to the bead complexes being adsorbed on 200 inner wall of sampling hammerhead；Washing step S22, it is remote in magnetic part
Under the demagnetizing state that direction from sampling hammerhead 200 retracts, sampling hammerhead 200 draws the cleaning solution in sample container 3 and anti-
Compressing movement is executed again, and to clean bead complexes, later under for magnetic state, sampling hammerhead 200 spues except multiple containing magnetic bead
Close the solution of object；Elution step S23, under demagnetizing state, sampling hammerhead 200 draws the eluent and anti-in sample container 3
Compressing movement is executed again, so that magnetic bead and nucleic acid separation in bead complexes；Nucleic acid extraction step S24, for magnetic state
Under, sampling hammerhead 200 will be except the nucleic acid containing magnetic bead spues and saves to sample container 3；Sampling hammerhead unloading step, rifle
Sampling hammerhead 200 is offloaded to scrap area by head moving device 41.Here, optionally, in cleavage step S21, in sampling hammerhead
It further include protein digestibility step S25 after 200 draw the solution containing bead complexes, under demagnetizing state, sampling hammerhead
Solution containing bead complexes is spued into the protein digestibility enzyme solutions in sample container 3 and executes suction repeatedly by 200
Movement is spat, with the protein in digestion solution, later under for magnetic state, sampling hammerhead 200, which spues to remove, contains bead complexes
Solution, to complete cleavage step S21.Cleavage step S21 to nucleic acid extraction step S24 and institute as above in second step S2
It is identical to nucleic acid extraction step S24 to state the cleavage step S21 referred in method for extracting nucleic acid, herein in order to avoid repetition omission pair
Its explanation and possessed function and effect.Although in addition, being adopted in the second step S2 of the sample processing method of the disclosure
The step-by-step system retreated with the direction by microscope carrier with edge in contrast to microscope carrier moving direction Z1, so that the nucleic acid extracted is placed in sample
In the most nucleic acid holding tank of front side of this container 3, in order to which subsequent second mechanical arm 7 executes the operation of dispensing nucleic acid, but this
Open it's not limited to that, and microscope carrier driven in translation structure 12 may be set to be, and microscope carrier 2 can be driven in the second working region B
It is advanced along microscope carrier moving direction Z1 with step-by-step movement, in the case, cell pyrolysis liquid holding tank, protein digestibility enzyme solutions accommodate
Slot, cleaning solution holding tank, eluent holding tank and nucleic acid holding tank are on sample container along in contrast to microscope carrier moving direction Z1
Direction be sequentially arranged, the nucleic acid extracted is then placed in the nucleic acid holding tank positioned at rear side of sample container.
Optionally, microscope carrier transport establishment 1 includes arranging in at least partly described working region along height direction H perforation
Upper layer microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112, the first working region A, the second working region B and
Three working region C are sequentially arranged on upper layer microscope carrier shipping platform 111 along microscope carrier moving direction Z1, and working region further includes being used for
The initial storage area D of microscope carrier of multiple microscope carriers 2 is stored, the initial storage area D of microscope carrier is arranged in upper layer microscope carrier shipping platform
111 and lower layer's microscope carrier shipping platform 112 side and be located at close to the region of the first working region A, microscope carrier driving mechanism includes
Microscope carrier driven in translation structure 12 and microscope carrier go up and down driving structure 13, and the setting of microscope carrier driven in translation structure 12 is flat in the transport of upper layer microscope carrier
On platform 111, and for driving microscope carrier 2 to adjust position along microscope carrier moving direction Z1 on upper layer microscope carrier shipping platform 111, so that
Microscope carrier 2 is moved to corresponding working region and successively executes first step S1 to third step S3, and microscope carrier goes up and down driving structure 13
It is arranged on lower layer's microscope carrier shipping platform 112, and for driving microscope carrier 2 to go up and down along height direction H, so that microscope carrier 2 is on upper layer
Switching position between microscope carrier shipping platform 111 and lower layer's microscope carrier shipping platform 112, after third step S3, sample process side
Method further includes microscope carrier return step S4, and the microscope carrier 2 of third working region C is transferred to microscope carrier using microscope carrier lifting driving structure 13
Initial storage area D.Here, microscope carrier driven in translation structure 12 and microscope carrier lifting driving structure 13 can using with it is as described above
The microscope carrier driven in translation structure 12 referred in microscope carrier transport establishment 1 construction identical with the microscope carrier lifting structure of driving structure 13,
By structure as described above and sample processing method, sample processing device, can be in upper layer microscope carrier when executing sample process
Most sample process operation is executed on shipping platform 111, i.e., successively executes the first step on upper layer microscope carrier shipping platform 111
Rapid S1, second step S2 and third step S3, the microscope carrier 2 after completing third step S3 can go up and down driving knot by microscope carrier
Structure 13 is transferred to the initial position of the initial storage area D of microscope carrier, that is, for example, as shown in Figures 2 and 3, microscope carrier 2 is provided with six
In the case where a, wherein three microscope carriers 2 can be arranged in the initial storage area D of microscope carrier of upper layer microscope carrier shipping platform 111, remaining
Three microscope carriers 2 can be arranged in the initial storage area D of microscope carrier of lower layer's microscope carrier shipping platform 112.In the case, with reference to Fig. 5
And Fig. 6, if first microscope carrier 2 for being located at the most front side of upper layer microscope carrier shipping platform 111 is sequentially completed first step S1 to the
After three step S3, driving structure 13 is gone up and down for first microscope carrier 2 from the third work of upper layer microscope carrier shipping platform 11 by microscope carrier
Make the initial storage area D's of microscope carrier that region C is transferred to upper layer microscope carrier shipping platform 111 via lower layer's microscope carrier shipping platform 112
Initial position, that is, each microscope carrier 2 is completed entire sample process using closed-loop shaped movement routine along microscope carrier moving direction Z1 and made
The initial position of the initial storage area D of microscope carrier is returned to after industry, consequently facilitating the sample process of subsequent cycle.Here, for carrying
The position disclosure of the initial storage area D of platform is not limited to the above embodiment, for example, the initial storage area D of microscope carrier
It can all be arranged on lower layer's microscope carrier shipping platform 112.Thus it is mainly used to arrangement on lower layer's microscope carrier shipping platform 112 to deposit
Put the initial storage area D of microscope carrier of microscope carrier 2 so that microscope carrier 2 three-dimensional arrangement upper layer microscope carrier shipping platform 111 and under
Respectively divide the work on layer microscope carrier shipping platform 112 and clearly execute corresponding operation, and then effectively improves sample process operating efficiency.
In addition, as described in above-mentioned sample processing device, optionally, as shown in fig. 7, working region further includes for the
The first consumptive material storage area E that sample dispenses in one step S1, the first lifting arm 6 are arranged in the first consumptive material storage area E, should
First consumptive material storage area E is accommodated with the sampling hammerhead storage area E1 for storing sampling hammerhead 200 and for storing
The probe tube storage area E2 of the probe tube 300 of sample, the first consumptive material storage area E are arranged in upper layer microscope carrier shipping platform 111
Top that is upper and being located at the initial storage area D of microscope carrier, first mechanical arm 6 are arranged in the first consumptive material storage area E and are arranged as,
It can be moved back and forth between the first working region A and the first consumptive material storage area E, can be taken on the first working region A
It is loaded in the sample dispensed in probe tube 300 the sample container 3 on microscope carrier 2 Nei.As a result, each in microscope carrier transport establishment 1
A working region arrangement is more rationalized, by the top that the first consumptive material storage area E is disposed adjacent to the first working region A
Position, after making first mechanical arm 6 load the sampling hammerhead 200 in the E1 of sampling hammerhead storage area in first step S1,
Be moved in the E2 of probe tube storage area and draw the sample in probe tube 300, be moved to the first working region A later and to load
Injecting sample in each sample container 3, thus more quickly and easily executes first step S1, and be easily achieved on platform
Automate sample process operation.
To sum up, sample processing method is described in detail, here, in order to more comprehensively, clearly demonstrate sample
Each step of processing, enumerates specific example herein to illustrate all processes of the sample process of the disclosure, but this is not used as limiting
Determine the scope of the present disclosure, but is used only for explaining the sample processing method of the disclosure.Here, in applicable sample processing device
All the relevant technologies features as described above are provided with, i.e., include being provided with upper layer load as described above in the sample processing device
The microscope carrier transport establishment 1 of platform shipping platform 111 and lower layer's microscope carrier shipping platform 112, microscope carrier guide frame, microscope carrier location structure,
It is provided with the microscope carrier 2 of fixing seat 24 and sliding seat 25 and with the pipette tips mobile device for being provided with pipette tips ejection releasing mechanism 5
41 and magnetic bead transfer device 42 nucleic acid extraction mechanism 4.In the sample processing device, set on upper layer microscope carrier shipping platform 111
It is equipped with partial region, the first working region A, the second working region B, third working region C, of the initial storage area D of microscope carrier
One consumptive material storage area E and the second consumptive material storage area F, the partial region of the initial storage area D of microscope carrier are positioned close to
The position of one working region A is provided with another part region of the initial storage area D of microscope carrier on lower layer's microscope carrier shipping platform 112
And the underface in a part of region of the initial storage area D of microscope carrier on upper layer microscope carrier shipping platform 111, the first consumption
Material storage area E is located at the top of the initial storage area D of microscope carrier, the second consumptive material storage area on upper layer microscope carrier shipping platform 111
Domain F is arranged in another side position of the close third working region C of upper layer microscope carrier shipping platform 111, and on upper layer, microscope carrier transport is flat
It is provided with microscope carrier driven in translation structure 12 on platform 111, microscope carrier lifting driving structure is provided on lower layer's microscope carrier shipping platform 112
13 and microscope carrier return driving structure 18.In addition, there are six microscope carriers 2 for setting altogether in above-mentioned sample processing device, wherein three loads
Platform 2 can be arranged in the initial storage area D of microscope carrier of upper layer microscope carrier shipping platform 111, and excess-three microscope carrier 2 can be arranged in down
The initial storage area D of microscope carrier of layer microscope carrier shipping platform 112.Here, on microscope carrier moving direction Z1 from be located at upper layer microscope carrier
The microscope carrier of the most front side (i.e. the top side for being located at upper layer microscope carrier shipping platform 111 in Fig. 5) of shipping platform 111 is carried to lower layer is located at
The microscope carrier of the most rear side (i.e. the top side for being located at lower layer's microscope carrier shipping platform 112 in Fig. 6) of platform shipping platform 111 is successively defined as
First microscope carrier to the 6th microscope carrier.In addition, can be set on each microscope carrier, there are four sample containers 3, and each sample container 3
Four groups of holding tank groups for extracting four nucleic acid inside can be set, thus sample processing device as described above is being run
When can disposably execute the processing of 96 samples, therefore use the deep-well plates 100 in 96 holes accordingly to detect 96 nucleic acid.
It is specially as follows to its sample process process with reference to Figure 43 and Figure 44 based on sample processing device as described above.
Before running sample processing device, as shown in Figures 2 and 3, each microscope carrier 2 can be by sliding seat 25 from microscope carrier
Transport establishment 1 corresponds to the microscope carrier formed on the initial storage area D of microscope carrier and picks and places 14 stretching of opening, respectively into each microscope carrier 2
It is accordingly put into sample container 3, has wherein been put into cell pyrolysis liquid in each holding tank of sample container 3, protein disappears
Change enzyme solutions, cleaning solution and eluent and the opening that each sample holding tank is closed by sealing materials such as aluminium foils, here, can
After internal standard receiving pipe to be placed on each microscope carrier 2 (such as sliding seat 25) as needed, sliding seat 25 is retracted into original
Position, runs sample processing device later.
Firstly, when operation sample processing device, as shown in fig. 7, the internal standard dispensing step S11 and third step of first step S1
Detection reagent dispensing step S31 in rapid S3 is synchronously executed.Pass through first mechanical arm 6 to the first of the first working region A
In microscope carrier while dispensing internal standard, detection examination is dispensed into the deep-well plates 100 of deep-well plates storage area F2 by second mechanical arm 7
Agent, specifically, second mechanical arm 7 be moved to dispensing pipette tips storage area F1 and load dispensing pipette tips 400 after, be moved to detection
Reagent accommodates pipe storage area F3 and draws the detection reagent in detection reagent receiving pipe 500, is moved to deep-well plates storage later
Region F2 and inject detection reagent into deep-well plates 100.
In first step S1, the first driving motor 121 of starting microscope carrier driven in translation structure 12 operates and drives first
First transmission belt 123 of transmission component rotates, and drive activity trolley 122 is moved on trolley guide rail 126 corresponding to first
The position of microscope carrier is powered off by the second magnet plunger 81 on movable trolley 122 so that the second magnet plunger 81 pushes away in this case
Bar is stretched out and is snapped onto the locking structure 21 of the first microscope carrier, thus the first microscope carrier of locking and movable trolley 122.Later by the
The rotation of one transmission belt 123 and drive activity trolley 122 and the first microscope carrier are moved to the first working region A together, upper layer at this time
On second positioning seat 15 of microscope carrier shipping platform 111, the trough type photoelectric sensor 16 corresponding to the first working region A detects
When the position of the block 222 on the first positioning seat 22 of one microscope carrier, according to the signal that trough type photoelectric sensor 16 detects, upper layer
It is located at the first electricity with 16 corresponding position of groove profile photoelectrical position sensor on first working region A of microscope carrier shipping platform 111
Magnetic bar 151 stretches out and cooperates with the locating slot 221 on the first microscope carrier, so that the first microscope carrier is navigated to upper layer microscope carrier shipping platform
111 the first working region A executes first step S1.The internal standard dispensing step S11 of first step S1 is specifically first carried out, the
After one mechanical arm 6 loads the sampling hammerhead 200 in the E1 of sampling hammerhead storage area, draw internal standard accommodate internal standard in pipe and to the
Internal standard is dispensed in each sample container 3 on the first microscope carrier of one working region A, would be used for dispensing interior target later and take
Sample pipette tips 200 are discarded.Later, after the sampling hammerhead 200 that first mechanical arm 6 is reloaded in the E1 of sampling hammerhead storage area,
Be moved in the E2 of probe tube storage area and draw the sample in probe tube 300, be moved to the first working region A later and to the
Injecting sample in each sample container 3, can would be used for the unloading of sampling hammerhead 200 of sample injection later on one microscope carrier
To the sampling gun holding tank of each sample container 3.Here, in order to improve nucleic acid extraction efficiency, it is interior in first step S1
Mark and sample can be directly injected into the cell cracking liquid bath of sample container 3, that is, accommodate the cell cracking containing magnetic bead
In the slot of liquid, this part steps had both belonged to the sample injection step of first step S1, also belonged to the cracking in method for extracting nucleic acid
First half step in step S21, in this case, it is possible to which saving in cleavage step S21 sampling hammerhead 200 draws sample and to sample
The step of sample is added in the cell pyrolysis liquid containing magnetic bead in this container 3.After completing first step S1, upper layer microscope carrier
Shipping platform 111 is powered corresponding to the first magnet plunger 151 of the first working region A, passes through the magnetic generated in the first magnet plunger 151
Power makes the pusher retracted and is detached from the locating slot 221 of the first microscope carrier, enables the first microscope carrier that movable trolley 122 is followed to move
It is dynamic.Later, the 123 drive activity trolley 122 of the first transmission belt and the first microscope carrier of microscope carrier driven in translation structure 12 are moved to together
Second working region B, at this time on the second positioning seat 15 of upper layer microscope carrier shipping platform 111, the slot corresponding to the second working region B
When type photoelectric sensor 16 detects the position of the block 222 on the first positioning seat 22 of the first microscope carrier, according to groove profile photoelectric transfer
The signal that sensor 16 detects, is located on the second working region B of upper layer microscope carrier shipping platform 111 and the groove profile optoelectronic position passes
First magnet plunger 151 of 16 corresponding position of sensor stretches out and cooperates with the locating slot 221 on the first microscope carrier, thus by the first microscope carrier
Navigate to the second working region B of upper layer microscope carrier shipping platform 111.The movable trolley 122 for being detached from the first microscope carrier is moved to upper layer
The position corresponding to the second microscope carrier of microscope carrier shipping platform 111, and it is latter with being moved to the first workspace with the second microscope carrier locking
Domain A, and the second microscope carrier is navigated into the first working region A, so that the second microscope carrier executes first step S1, under this microscope carrier
Movable trolley 122 is detached from the second microscope carrier and is moved to the position corresponding to the first microscope carrier of the second working region B, and with the first load
Stand lock stops.In the case, upper layer microscope carrier shipping platform 111 is powered corresponding to the first magnet plunger 151 of the second working region B,
The locating slot 221 for making pusher retracted be detached from the first microscope carrier by the magnetic force generated in the first magnet plunger 151, so that first carries
Platform can follow movable trolley 122 mobile, and thus the first microscope carrier executes second step S2.The first microscope carrier of second working region B
Follow movable trolley 122 movable together in the state of the cooperation of movable trolley 122, specifically, in second step S2, first is carried
Platform follows movable trolley 122 along the direction of the separate third working region C in contrast to microscope carrier moving direction Z1 (i.e. close to the first work
Make the direction of region A) advanced with step-by-step movement, in step-by-step movement traveling process by lifting action with pipette tips mobile device 41 and
The cooperation of the expanding-contracting action of magnetic bead transfer device 42 is sequentially inserted into cell pyrolysis liquid holding tank, the protein of sample container 3
It digests in enzyme solutions holding tank, cleaning solution holding tank and eluent holding tank and executes and extract core contained in each sample
Acid, and nucleic acid is stored into being located on the foremost side in nucleic acid holding tank to sample container 3.
Later, the 123 drive activity trolley 122 of the first transmission belt and the first microscope carrier of microscope carrier driven in translation structure 12 move together
It moves to third working region C.On second positioning seat 15 of upper layer microscope carrier shipping platform 111, the slot corresponding to third working region C
When type photoelectric sensor 16 detects the position of block 222 of the first microscope carrier, the letter that is detected according to trough type photoelectric sensor 16
Number, it is located at and 16 corresponding position of groove profile photoelectrical position sensor on the third working region C of upper layer microscope carrier shipping platform 111
First magnet plunger 151 stretches out and cooperates with the locating slot 221 on the first microscope carrier, so that the first microscope carrier is navigated to upper layer microscope carrier fortune
The third working region C of defeated platform 111 passes through the second magnet plunger 81 at this point, the second magnet plunger 81 of movable trolley 122 is powered
The magnetic force of interior generation make the pusher retracted of the second magnet plunger 81 and from the locking structure 21 for being detached from the first microscope carrier, thus unlock the
One microscope carrier and movable trolley 122.Here, the movable trolley 122 for being detached from the first microscope carrier is moved to upper layer microscope carrier shipping platform 111
First working region A corresponds to the position of the second microscope carrier, and latter with successively with the second microscope carrier locking of completion first step S1
Be moved to the second working region B, and after the second microscope carrier is navigated to the second working region B, movable trolley be detached from the second microscope carrier and
It is moved to the position corresponding to third microscope carrier of upper layer microscope carrier shipping platform 111, and latter with being moved to third microscope carrier locking
First working region A, third microscope carrier navigate to the first working region A, so that third microscope carrier executes first step S1.In this state
Under, movable trolley 122 is detached from third microscope carrier and is moved to the position corresponding to the second microscope carrier of the second working region B, and with the
Two microscope carrier lockings are made by the elastic-restoring force of 151 inner spring of the first magnet plunger of the second working region B in this case
Pusher retracted and the locating slot 221 for being detached from the second microscope carrier enable the second microscope carrier to follow movable trolley 122 mobile, and thus the
Two microscope carriers execute second step S2.That is, at this point, third microscope carrier, the second microscope carrier and the first microscope carrier are in corresponding first work
First step S1, second step S2 and third are synchronously executed in region A, the second working region B and third working region C
Step S3, to significantly improve the operating efficiency of entire sample process.
In addition, executing the nucleic acid dispensing operation of third step S3 positioned at the first microscope carrier of third working region C.First microscope carrier
When the nucleic acid for executing third step S3 dispenses operation, it is preferable that the first microscope carrier executes synchronous the executed when first step S1
The detection reagent dispensing step S31 of three step S3 has been fulfiled ahead of schedule or has by chance been completed, and this saves the first microscope carrier in third
Time needed for waiting detection reagent dispensing step S31 to complete in the C of working region.In third step S3, second mechanical arm 7 is moved
It moves to dispensing pipette tips storage area F1 and after loading dispensing pipette tips 400, moves third working region C and draw the first microscope carrier
Nucleic acid in each 3 amplifying nucleic acid holding tank of sample container is moved to deep-well plates storage area F2 and into deep-well plates 100 later
Inject nucleic acid.
The first microscope carrier of third working region C executes microscope carrier return after completing the nucleic acid dispensing operation of third step S3
Step S4, that is, driving structure 13 is gone up and down by microscope carrier and microscope carrier return driving structure 18 is moved to lower layer's microscope carrier shipping platform 112
Corresponding position on, for example, can be moved to lower layer's microscope carrier shipping platform 112 close to the 6th microscope carrier position on, Zhi Hou
One microscope carrier is finally transferred to the initial position of the initial storage area D of microscope carrier of upper layer microscope carrier shipping platform 111.
Similarly with the above-mentioned course of work, the 4th microscope carrier on lower layer's microscope carrier shipping platform 112, the 5th microscope carrier and the 6th carry
Platform is moved into upper layer microscope carrier shipping platform by the cooperation that microscope carrier goes up and down driving structure 13 and microscope carrier return driving structure 18
On 111, and the first working region A, the second work are successively moved to by the movable trolley 122 of microscope carrier driven in translation structure 12
Make region B and third working region C successively executes first step S1, second step S2 and third step S3, later finally
Lower layer's microscope carrier shipping platform 112 is moved to by the cooperation that microscope carrier goes up and down driving structure 13 and microscope carrier return driving structure 18
Thus the initial storage area D of microscope carrier completes the processing of 96 samples on six microscope carriers.
To sum up, by sample processing device as described above and sample processing method, multiple microscope carriers be can be realized respective
Accordingly step is synchronously executed respectively in corresponding working region, also, when bringing into operation sample processing device, the first step
Rapid S1 and detection reagent dispensing step S31 may be performed simultaneously, and thus, it is possible to significantly improve the sample process of entire cycle period
Efficiency realizes full-automatic streamlined operation.In addition, since the microscope carrier transport establishment 1 in sample processing device is using multilayer load
The three-dimensional arrangement architecture of platform shipping platform 11, therefore area needed for the arrangement of sample processing device can be effectively reduced, it saves
Arrangement space.
The preferred embodiment of the disclosure is described in detail in conjunction with attached drawing above, still, the disclosure is not limited to above-mentioned reality
The detail in mode is applied, in the range of the technology design of the disclosure, a variety of letters can be carried out to the technical solution of the disclosure
Monotropic type, these simple variants belong to the protection scope of the disclosure.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the disclosure to it is various can
No further explanation will be given for the combination of energy.
In addition, any combination can also be carried out between a variety of different embodiments of the disclosure, as long as it is without prejudice to originally
Disclosed thought equally should be considered as disclosure disclosure of that.
Claims (15)
1. a kind of sample processing device, which is characterized in that the sample processing device includes microscope carrier transport establishment (1) and movably
Multiple microscope carriers (2) on the microscope carrier transport establishment (1) are set, multiple samples are provided on each microscope carrier (2) and are accommodated
Device (3), each sample container (3) is interior to accommodate useful multiple reagent solutions in processing sample, the microscope carrier transport establishment (1)
On be disposed with multiple working regions, be provided with first mechanical arm (6) and second mechanical arm (7) on the microscope carrier transport establishment (1),
The working region includes at least: the first working region (A), the first mechanical arm (6) are positioned close to described first
The position of working region (A), and the first mechanical arm (6) is set as, it can be along the longitudinal direction for being parallel to microscope carrier moving direction (Z1)
Direction (X1) and position is adjusted perpendicular to the transverse direction (X2) of the microscope carrier moving direction (Z1), and it can be along short transverse
(H) it goes up and down, for dispensing sample to each sample container of the microscope carrier (2) in first working region (A)
(3) in；
Second working region (B), second working region (B) is interior to be provided with nucleic acid extraction mechanism (4), the nucleic acid extraction mechanism
(4) it is configured to move along the direction close to or far from the microscope carrier (2), for extracting the sample in sample container (3)
Nucleic acid contained in this, and the nucleic acid of extraction is stored to each sample container (3)；
Third working region (C), the second mechanical arm (7) are positioned close to the position of the third working region (C), and should
Second mechanical arm (7) is set as, and can adjust position along the longitudinal direction (X1) and the transverse direction (X2), and being capable of edge
Short transverse (H) lifting, is examined with the nucleic acid for dispensing detection reagent and extraction respectively into multiple deep-well plates (100)
Nucleic acid is surveyed,
Each microscope carrier (2) can be along the microscope carrier moving direction (Z1) successively from first work by microscope carrier driving mechanism
Make region (A) and move to second working region (B) and the third working region (C), to execute relevant work.
2. sample processing device according to claim 1, which is characterized in that the nucleic acid extraction mechanism (4) includes: pipette tips
The microscope carrier transporter can be arranged in up and down in mobile device (41), the pipette tips mobile device (41) along the short transverse (H)
On structure (1), for driving the sampling hammerhead (200) being separatably arranged on the pipette tips mobile device (41) along the height
The shift position direction (H) is spent, and sampling hammerhead (200) is enabled to adsorb liquid or discharge in the sample container (3)
Liquid in the sampling hammerhead (200)；
Magnetic bead transfer device (42), which includes magnetic part, the magnetic part can along close to or far from
The direction of sampling hammerhead (200) is telescopically arranged on the microscope carrier transport establishment (1), so that magnetic part has to sampling gun
Magnetic bead in head (200) provides going for the magnetic force for the magnetic bead of magnetic force acted in sampling hammerhead (200) for magnetic state and removing
Magnetic state.
3. sample processing device according to claim 2, which is characterized in that the pipette tips mobile device (41) includes setting
Pedestal (411) on the microscope carrier transport establishment (1) and the pedestal can be set up and down along the short transverse (H)
(411) pipette tips on eject releasing mechanism (5), and it includes for installing sampling hammerhead (200) which, which ejects releasing mechanism (5),
Sampling gun (51), sampling gun actuator (52) and ejection release piece (53), the driving portion of the sampling gun actuator (52) and institute
The telescopic rod (511) for stating sampling gun (51) connects and for driving the telescopic rod (511) flexible, so that the sampling gun
(51) suction of imbibition is provided for the sampling hammerhead (200) or the pressure of drain, the ejection release piece (53) are mounted on institute
It states on sampling gun actuator (52) and the sampling hammerhead (200) is enabled to separate with the sampling gun (51),
Optionally, the sampling gun (51) includes main body (512) and multiple telescopic rods (511), main body (512) setting
On the fixed pedestal (521) of the sampling gun actuator (52), and there is the both ends open for the telescopic rod (511) insertion
Multiple telescopic rod channels (513), the driving portion at the top of each telescopic rod (511) and the sampling gun actuator (52)
Connection, and each telescopic rod (511) can be telescopically inserted by the driving of the driving portion along short transverse (H)
In corresponding telescopic rod channel (513), the main body (512) corresponds to each telescopic rod channel (513)
Bottom be respectively structured as with the pipette tips interconnecting piece (514) of the corresponding sampling hammerhead (200) connection, the ejection is de-
Off member (53) is configured to move relative to each pipette tips interconnecting piece (514), to enable to each sampling gun
Head (200) is separated with corresponding pipette tips interconnecting piece (514),
Optionally, the ejection release piece (53) includes that can be supported on up and down the sampling gun along the short transverse (H)
Ejection disengaging plate (531) on the fixed pedestal (521) of actuator (52), the ejection disengaging plate (531) is in the short transverse
(H) it is located at the lower section of the main body (512) on, and is formed with multiple pipette tips interconnecting piece insertions on the ejection disengaging plate (531)
The pipette tips linkage section (515) in hole (532), each pipette tips interconnecting piece (514) respectively accordingly penetrates through the pipette tips interconnecting piece
Insertion hole (532) and the side for being exposed to ejection disengaging plate (531), the driving portion setting of the sampling gun actuator (52)
For that the ejection disengaging plate (531) can be driven along sampling hammerhead under the retracted mode that the telescopic rod (511) retract
(200) off-direction squeezes the sampling hammerhead (200) on each pipette tips interconnecting piece (514),
Optionally, the ejection release piece (53) include support plate (533) and with it is described support plate (533) and it is described ejection take off
Connecting rod (534) from plate (531) connection, it is described support plate (533) be located at the driving portion of the sampling gun actuator (52) with
It is described to support plate (533) and the ejection disengaging plate (531) by the connecting rod (534) between the main body (512)
It is arranged on the fixed pedestal (521) up and down along the short transverse (H), driving portion energy under the retracted mode
It is enough to be abutted with the plate (533) of supporting, to push the ejection release piece (53) to move along the off-direction, the connecting rod
(534) it is resiliently supported on the fixed pedestal (521) by the reset spring (535) being set in the connecting rod (534), institute
It states the both ends of reset spring (535) and supports plate (533) and the fixed pedestal (521) abuts with described respectively, for providing
Drive ejection release piece (53) to return back to the elastic-restoring force of initial position always,
Optionally, the sampling gun (51) has been positioned apart from multiple, the plate (533) and described supported in transverse direction (X2)
It ejects disengaging plate (531) to extend along the transverse direction (X2) respectively, the connecting rod (534) is multiple and respectively along the cross
It is arranged in the mode for being separated with a sampling gun (51) between direction (X2) and described supports plate (533) and the ejection disengaging plate
(531) between, so that described support connecting rod described in plate (533), the ejection disengaging plate (531) and adjacent every two
(534) sampling gun layout area is respectively constituted between, the main body (512) of each sampling gun (51) is located at the sampling gun cloth
It sets in region,
Optionally, the sampling gun actuator (52) includes the fixed pedestal (521), is arranged on the fixed pedestal (521)
Sampling gun driving motor (522), be connected with the transmission of the output shaft of the sampling gun driving motor (522) and be provided with the drive
The sampling gun transmission mechanism in dynamic portion, converts the driving portion for the rotary motion of the sampling gun driving motor (522)
Linear motion,
Optionally, the sampling gun transmission mechanism be screw-and-nut mechanism, including with the sampling gun driving motor (522)
Output axis connection and the first screw rod (523) for being pivotally supported on the fixed pedestal (521) and can be along the height
Degree direction (H) is movably arranged at the first nut (524) on first screw rod (523), and the driving portion is is set in
The driving baffle (525) on the outer peripheral surface of the first nut (524) is stated, is formed on the driving baffle (525) described for clamping
The holding tank (526) on the head of telescopic rod (511).
4. sample processing device according to claim 2, which is characterized in that the magnetic bead transfer device (42) includes setting
Magnetic bead transfer device fixing seat (421), setting on the position of microscope carrier transport establishment (1) close to sampling hammerhead (200) is in institute
The magnetic part actuator and magnetic part movable plate (422) in magnetic bead transfer device fixing seat (421) are stated, the magnetic part is mobile
Plate (422) is arranged on the magnetic bead transfer device fixing seat (421), and connect with the magnetic part actuator with can be along leaning on
Flexible close or remote from the direction of the sampling hammerhead (200), the magnetic part is magnet (423) and is mounted on the magnetic part shifting
The side of movable plate (422) close the sampling gun (51),
Optionally, the magnetic part actuator includes magnetic part driving motor (426) and magnetic part bolt and nut mechanism, the magnetic
Property part driving motor (426) be arranged on the magnetic bead transfer device fixing seat (421), magnetic part bolt and nut mechanism
The output axis connection of magnetic part screw rod (427) and the magnetic part driving motor (426), the magnetic of magnetic part bolt and nut mechanism
Property part nut (428) is connect with the magnetic part movable plate (422),
Optionally, the telescopic direction extension along the magnetic part movable plate (422) is formed on the magnetic part movable plate (422)
Long guiding hole (424), be provided with for being inserted into and being installed to the long guiding hole (424) on the magnetic bead transfer device (42)
Interior positioning screw (425), the magnetic part movable plate (422) pass through the long guiding hole (424) and the positioning screw
(425) cooperation is telescopically disposed on the magnetic bead transfer device (42).
5. sample processing device according to claim 1, which is characterized in that the microscope carrier transport establishment (1) includes along height
Spend direction (H) arrangement multilayer microscope carrier shipping platform (11), first working region (A), second working region (B) and
The third working region (C) is arranged in any one in microscope carrier shipping platform (11) described in multilayer, the microscope carrier driving
Mechanism includes microscope carrier driven in translation structure (12) and microscope carrier lifting driving structure (13), and the microscope carrier driven in translation structure (12) sets
It sets at least one of described microscope carrier shipping platform (11), and for driving the microscope carrier (2) in the corresponding microscope carrier
Position is adjusted along microscope carrier moving direction (Z1) in the working region of shipping platform (11), microscope carrier lifting driving structure (13) sets
Set in the microscope carrier shipping platform (11) remaining at least one on, and for driving the microscope carrier (2) along short transverse (H)
Lifting, so that the microscope carrier (2) switching position between each layer microscope carrier shipping platform (11).
6. sample processing device according to claim 5, which is characterized in that the microscope carrier transport establishment (1) includes along height
Spend the upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112) of direction (H) perforation ground arrangement, first work
Make region (A), second working region (B) and the third working region (C) along microscope carrier moving direction (Z1) successively cloth
It sets on the upper layer microscope carrier shipping platform (111), microscope carrier driven in translation structure (12) setting is transported in the upper layer microscope carrier
On defeated platform (111), microscope carrier lifting driving structure (13) is arranged on lower layer's microscope carrier shipping platform (112), described
Working region further includes the initial storage area of microscope carrier (D) for storing multiple microscope carriers (2), the initial storage area of microscope carrier
At least part region in domain (D) is arranged on lower layer's microscope carrier shipping platform (112).
7. sample processing device according to claim 6, which is characterized in that microscope carrier driven in translation structure (12) setting
For that can drive the microscope carrier (2) in second working region (B) along separate in contrast to the microscope carrier moving direction (Z1)
The direction of the third working region (C) is advanced with step-by-step movement, with can be by moving with the pipette tips of the nucleic acid extraction mechanism (4)
The cooperation of the expanding-contracting action of the lifting action and magnetic bead transfer device (42) of dynamic device (41) is extracted the sample and is accommodated
Nucleic acid contained in sample in device (3) simultaneously stores the nucleic acid of extraction to each sample container (3).
8. sample processing device according to claim 6, which is characterized in that the initial storage area of microscope carrier (D) is respectively
It is arranged in the side of the upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112) and is located at close to institute
The region of the first working region (A) is stated, the microscope carrier transport establishment (1) corresponds on the side wall of the initial storage area of microscope carrier (D)
It is formed with and is used for so that the microscope carrier that the microscope carrier (2) is stretched out or retracted picks and places opening (14), the microscope carrier (2) is set as, in institute
Stating the initial storage area of microscope carrier (D) can be stretched by microscope carrier pick-and-place opening (14) relative to the microscope carrier transport establishment (1)
Out or retract,
Optionally, the working region further includes the first consumptive material storage dispensed for the sample of first working region (A)
Region (E), the first lifting arm (6) setting is in the first consumptive material storage area (E), the first consumptive material storage area (E) tool
There is sampling hammerhead storage area (E1) for storing sampling hammerhead (200) and for storing the probe tube for accommodating sample
(300) probe tube storage area (E2), first consumptive material storage area (E) are arranged in the upper layer microscope carrier shipping platform
(111) top on and positioned at the initial storage area of the microscope carrier (D), first mechanical arm (6) setting is in first consumption
In material storage area (E) and be arranged as, can first working region (A) and first consumptive material storage area (E) it
Between move back and forth, with can be mounted in on the first working region (A) the sample container (3) on the microscope carrier (2) Nei dispense
Sample in probe tube (300).
9. sample processing device according to claim 6, which is characterized in that the working region further includes for third work
Make the second consumptive material storage area (F) of the dispensing detection reagent in region (C) and the nucleic acid of extraction, second lifting arm (7) sets
It sets in second consumptive material storage area (F), which has for storing dispensing pipette tips (400)
Dispensing pipette tips storage area (F1), the deep-well plates storage area (F2) for storing deep-well plates (100) and for store accommodate
The detection reagent for having the detection reagent of detection reagent to accommodate pipe (500) accommodates pipe storage area (F3), the second consumptive material storage
Region (F) is arranged the other side in the upper layer microscope carrier shipping platform (111) and is located at close to the third working region (C)
Region, the second mechanical arm (7) can be between the third working region (C) and second consumptive material storage areas (F)
It moves back and forth, with can be in multiple deep-well plates (100) into second consumptive material storage area (F) respectively described in dispensing
Detection reagent accommodate pipe (500) in detection reagent and, be located at the third working region (C) in be mounted in the microscope carrier (2)
On sample container (3) in nucleic acid, to detect nucleic acid.
10. sample processing device according to claim 6, which is characterized in that the microscope carrier driven in translation structure (12) sets
It sets on the side inner wall of the upper layer microscope carrier shipping platform (111), and including the first driving motor (121) and first drive
It the first connected transmission component of the output shaft transmission of dynamic motor (121) and is connect with first transmission component and can be with
The movable trolley (122) that the microscope carrier separatably cooperates, first transmission component are used for first driving motor
(121) rotary motion is converted to the linear movement of the movable trolley (122), to enable to the movable trolley (122)
The working region locating for the microscope carrier (2) is adjusted by the cooperation with the microscope carrier (2),
Optionally, first transmission component is that V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission are matched
Mechanism is closed,
Optionally, first transmission component is V belt translation matching mechanism, and including the first transmission belt (123), setting described
First transmission belt (123) side and with the first driving wheel (124) of the output axis connection of first driving motor (121) and
The first driven wheel (125) in the first transmission belt (123) other side is set, and the activity trolley (122) is mounted on described
On first transmission belt (123),
Optionally, in the upper layer microscope carrier shipping platform (111) in the downside inner wall section of first transmission belt (123)
It is provided with the trolley guide rail (126) extended along the microscope carrier moving direction (Z1), the activity trolley (122) is fixed on described the
In the downside band part of one transmission belt (123), the bottom of the activity trolley (122), which is provided with, is slideably led with the trolley
The second pulley component (127) of rail (126) cooperation,
Optionally, the second pulley component (127) is arranged by second pulley mounting base (128) in the movable trolley
(122) bottom, the second pulley mounting base (128) are located on the downside of first transmission belt (123) and pass through described second
Pulley assembly (127) is supported on the trolley guide rail (126),
Optionally, it is telescopically provided with stretching structure (8) on the movable trolley (122), the microscope carrier (2) corresponds to described
On the side side wall of movable trolley (122), it is provided with the locking structure being mutually locked for cooperating with the stretching structure (8)
(21),
Optionally, the stretching structure (8) include the second magnet plunger (81), second magnet plunger (81) have lockup state and
Unlocked state, in the lockup state, the second magnet plunger (81) power-off, so that the push rod of the second magnet plunger (81) stretches out
And it snaps on the locking structure (21) and microscope carrier (2) described in locking and the movable trolley (122)；In the unlock shape
State, second magnet plunger (81) are powered, to make the push rod contract by the magnetic force generated in second magnet plunger (81)
It returns and unlocks the microscope carrier (2) and the movable trolley (122), the locking structure (21) is formed to have and second electricity
The lock groove or locking hole of the shape of magnetic bar (81) cooperation,
Optionally, the stretching structure (8) further includes electromagnet (82), and the electromagnet (82) is two and is separately positioned on institute
State movable trolley (122) on the position of the second magnet plunger (81) two sides, microscope carrier (2) correspond to the electromagnet
(82) the electromagnet latch (28) for cooperating with the electromagnet (82) is formed on side wall, in the lockup state,
The electromagnet (82) is powered and the magnetic force by generating is adsorbed onto the electromagnet latch (28), to carry described in locking
Platform (2) and the movable trolley (122)；In the unlocked state, the electromagnet (82) powers off and is detached from the electromagnet lock
Stops (28), to unlock the microscope carrier (2) and the movable trolley (122),
Optionally, the microscope carrier transport establishment (1) includes being arranged in the side of the working region of the microscope carrier transport establishment (1)
On side wall and along the second positioning seat (15) that the microscope carrier moving direction (Z1) extends, for the microscope carrier (2) to be navigated to
Each working region has been positioned apart from multiple along the microscope carrier moving direction (Z1) on second positioning seat (15)
Two positioning regions, second positioning region are used to cooperate or divide with the first positioning region being arranged on the side side wall of microscope carrier (2)
From in the lockup state, all second positioning regions are with first position portion from so that the microscope carrier (2)
It is moved freely on the upper layer microscope carrier shipping platform (111) in the state of with described movable trolley (122) locking, described
Unlocked state, when first positioning region is located at the position opposite with any one the second positioning region in second positioning region,
First positioning region cooperates with corresponding second positioning region so that the microscope carrier (2) with the movable trolley
(122) it is navigated on the upper layer microscope carrier shipping platform (111) in the state of unlocking,
Optionally, second positioning region is the first magnet plunger (151), and first positioning region is locating slot (221), described the
One magnet plunger (151) has working condition and off working state, and in the working condition, first magnet plunger (151) is powered off,
So that the push rod of the first magnet plunger (151) is stretched out and is snapped onto the locating slot (221), in the off working state, institute
The first magnet plunger (151) energization is stated, be detached from the pusher retracted by the magnetic force generated in the first magnet plunger (151)
The locating slot (221),
Optionally, it is provided with the first positioning seat (22) on the side side wall of the microscope carrier (2), locating slot (221) shape
At on first positioning seat (22), second positioning seat (15) is adjacent to the position of each first magnet plunger (151)
On, it is separately provided for detecting the position sensor of the position of first positioning seat (22), to pass in multiple positions
When a position sensor in sensor detects the position of first positioning seat (22), so that first magnet plunger
(151) cooperate in corresponding to the first magnet plunger of one position sensor and the locating slot (221),
Optionally, the position sensor is groove profile photoelectrical position sensor (16), the groove profile photoelectrical position sensor (16)
Slot towards the inside opening of the upper layer microscope carrier shipping platform (111), be formed with for wearing on first positioning seat (22)
Cross the block (222) of the slot of the groove profile photoelectrical position sensor (16).
11. sample processing device according to claim 6, which is characterized in that microscope carrier lifting driving structure (13) packet
It includes the lifting fixed pedestal (131) being arranged on lower layer's microscope carrier shipping platform (112) and the liter is liftably set
The lift cylinders (132) that drops on fixed pedestal (131) and can be detached from the microscope carrier (2) cooperation, so that microscope carrier (2) is in institute
Switching position between upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112) is stated,
Optionally, microscope carrier return driving structure (18), the load are provided on the inner sidewall of lower layer's microscope carrier shipping platform (112)
Platform return driving structure (18) is connect with the lifting fixed pedestal (131), and for driving the microscope carrier lifting driving structure
It is (13) mobile along microscope carrier moving direction (Z1) or in contrast to the direction of the microscope carrier moving direction (Z1),
Optionally, the microscope carrier return driving structure (18) include the second driving motor (181) and with second driving motor
(181) the second connected transmission component of output shaft transmission, the lifting fixed pedestal (131) are mounted on second transmission group
On the movable part of part, second transmission component is used to for the rotary motion of second driving motor (181) being converted to described
Microscope carrier goes up and down driving structure (13) along the linear movement of the microscope carrier moving direction (Z1), or in contrast to the microscope carrier movement side
Linear movement to the direction of (Z1),
Optionally, second transmission component is that V belt translation matching mechanism, gear auxiliary driving matching mechanism or screw pair gear transmission are matched
Mechanism is closed,
Optionally, second transmission component is V belt translation matching mechanism, and including connecting with the lifting fixed pedestal (131)
The second transmission belt (182), setting is in the second transmission belt (182) side and defeated with second driving motor (181)
The second driven wheel (184) of the second driving wheel (183) of axis connection, setting in the second transmission belt (182) other side out, institute
Lifting fixed pedestal (131) is stated to be fixed on second transmission belt (182),
Optionally, second transmission component is provided with two groups and is separately positioned on lower layer's microscope carrier shipping platform (112)
On the inner wall of two sides, two groups of second driven wheel (184) is mutually interlocked by transmission shaft (185),
Optionally, it is located at below second transmission belt (182) in the two sides inner wall of lower layer's microscope carrier shipping platform (112)
On position, it is respectively arranged with and extends along microscope carrier moving direction (Z1) and cooperate with the two sidewalls of lifting fixed pedestal (131)
Lifting fixed pedestal guide rail (133) so that the lifting fixed pedestal (131) drives the microscope carrier (2) in the lifting
Fixed pedestal guide rail moves on (133) and the microscope carrier (2) is made to return back to the initial storage area of the microscope carrier (D).
12. a kind of sample processing method using sample processing device described in any one of -11 according to claim 1, special
Sign is that the sample processing method includes:
First step (S1), microscope carrier (2) are moved to first working region (A) along microscope carrier moving direction (Z1), using described
First mechanical arm (6) dispenses sample in each sample container (3) on the microscope carrier (2) respectively；
Second step (S2), microscope carrier (2) are moved to from first working region (A) along the microscope carrier moving direction (Z1) described
Second working region (B) is extracted using the nucleic acid extraction mechanism (4) in the sample in the sample container (3) on microscope carrier (2)
Contained nucleic acid, and the nucleic acid of extraction is stored to each sample container (3)；
Third step (S3), microscope carrier (2) are moved to the third working region (C) from second working region (B), utilize institute
State second mechanical arm (7) has the sample in multiple deep-well plates (100) of detection reagent on the dispensing microscope carrier (2) to accommodate to dispensing
The nucleic acid stored in device (3) and detect nucleic acid,
It is adjacent on the microscope carrier moving direction (Z1) from first working region (A) to the third working region (C)
Every two microscope carrier (2) in be located at the microscope carrier (2) of front side and executing the first step (S1) appointing into third step (S3)
While a step of anticipating, the previous step of any one step is executed positioned at the microscope carrier (2) of rear side.
13. sample processing method according to claim 12, which is characterized in that the working region further includes the second consumptive material
Storage area (F), the second consumptive material storage area (F) have the dispensing pipette tips storage area for storing dispensing pipette tips (400)
(F1), for storing the deep-well plates storage area (F2) of deep-well plates (100) and for storing the detection examination for accommodating detection reagent
The detection reagent that agent accommodates pipe (500) accommodates pipe storage area (F3), and second consumptive material storage area (F) is arranged in the load
The region close to the third working region (C) of platform transport establishment (1), the second mechanical arm (7) are arranged described second
In consumptive material storage area (F) and it is arranged as, it can be in the third working region (C) and second consumptive material storage area (F)
Between move back and forth, can be dispensed respectively in multiple deep-well plates (100) into second consumptive material storage area (F)
Detection reagent in the detection reagent container (500) and, be located in the third working region (C) and be mounted in the microscope carrier
(2) nucleic acid in sample container (3) on,
In the third step (S3), in the second mechanical arm (7) to the deep-well plates of second consumptive material storage area (F)
(100) in front of nucleic acid dispensing step (S32) of dispensing nucleic acid, further include detection reagent dispensing step (S31), utilize described the
Two mechanical arms (7) dispense detection reagent into multiple deep-well plates (100) of second consumptive material storage area (F),
It is synchronous to execute detection reagent dispensing step (S31) while starting to execute the first step (S1),
Optionally, in the first step (S1), the first mechanical arm (6) loads sampling hammerhead (200) and draws sampling afterwards
It manages the sample in (300) and dispenses sample in each sample container (3) on the microscope carrier (2), will use later
It is placed in the sample container (3) in the sampling hammerhead (200) of dispensing sample,
Optionally, in the first step (S1), in each sample of the first mechanical arm (6) on the microscope carrier (2)
It further include internal standard dispensing step (S11), first machine in container (3) before sample dispensing step (S12) of dispensing sample
Tool arm (6) draws internal standard after loading pipette tips and dispenses internal standard in each sample container (3) on the microscope carrier (2),
It is discarded to would be used for dispensing interior target pipette tips later.
14. sample processing method according to claim 12 or 13, which is characterized in that nucleic acid extraction mechanism (4) packet
Include: the microscope carrier can be arranged in up and down along the short transverse (H) in pipette tips mobile device (41), the pipette tips mobile device (41)
In transport establishment (1), for driving sampling hammerhead (200) edge being separatably arranged on the pipette tips mobile device (41)
Short transverse (H) shift position, and sampling hammerhead (200) is enabled to adsorb the liquid in the sample container (3)
Or the liquid in the discharge sampling hammerhead (200)；
Magnetic bead transfer device (42), which includes can be along the side close to or far from sampling hammerhead (200)
To the magnetic part being telescopically arranged on the microscope carrier transport establishment (1), to enable to magnetic part to have to sampling hammerhead
(200) magnetic bead in provides the degaussing of the magnetic force that the magnetic bead in sampling hammerhead (200) is acted on for magnetic state and removing of magnetic force
State,
The microscope carrier driving mechanism includes microscope carrier driven in translation structure (12), for driving the microscope carrier (2) corresponding described
Position, and the microscope carrier driven in translation are adjusted along the microscope carrier moving direction (Z1) in the working region of microscope carrier shipping platform (11)
Structure (12) is set as, and in the second step (S2), the microscope carrier (2) can be driven in second working region (B)
It is advanced along as the direction far from the third working region (C) in contrast to the microscope carrier moving direction (Z1) with step-by-step movement,
The expanding-contracting action of lifting action and the magnetic bead transfer device (42) with the pipette tips mobile device (41) can be passed through
Cooperation is sequentially inserted into each holding tank of the sample container (3) and extracts the sample in the sample container (3)
Contained in nucleic acid, and the nucleic acid of extraction is stored to each sample container (3),
Optionally, the second step (S2) includes:
Sampling hammerhead loads step, and the pipette tips mobile device (41) loads sampling hammerhead (200)；
Cleavage step (S21), the sampling hammerhead (200) draw sample and contain magnetic bead into the sample container (3)
The sample is added in cell pyrolysis liquid, so that obtaining the bead complexes that adsorption has nucleic acid, the sampling after sample cracking
Pipette tips (200) draw the solution containing bead complexes, stretch out in the magnetic part close to the direction of the sampling hammerhead (200)
It is described under magnetic state, the sampling hammerhead (200) spues except the magnetic bead being adsorbed on the sampling hammerhead (200) inner wall is multiple
Close the solution except object；
Washing step (S22), under the demagnetizing state that the magnetic part is retracted far from the direction of the sampling hammerhead (200), institute
Sampling hammerhead (200) are stated to draw the cleaning solution in the sample container (3) and execute compressing movement repeatedly, it is multiple to clean magnetic bead
Close object, later described for magnetic state under, the sampling hammerhead (200), which spues, removes the solution containing bead complexes；
Elution step (S23), under the demagnetizing state, the sampling hammerhead (200) is drawn in the sample container (3)
Eluent simultaneously executes compressing movement repeatedly, so that magnetic bead and nucleic acid separation in bead complexes, the sampling hammerhead (200)
Draw the solution containing magnetic bead and nucleic acid；
Nucleic acid extraction step (S24), it is described for magnetic state under, the sampling hammerhead (200) will spit except the nucleic acid containing magnetic bead
Out and save to the sample container (3)；
The sampling hammerhead (200) is offloaded to scrap area by sampling hammerhead unloading step, the pipette tips mobile device (41),
Optionally, in the cleavage step (S21), the sampling hammerhead (200) draw the solution containing bead complexes it
It afterwards, further include protein digestibility step (S25), under the demagnetizing state, the sampling hammerhead (200) will be compound containing magnetic bead
The solution of object spues into the protein digestibility enzyme solutions in the sample container (3) and executes compressing movement repeatedly, to disappear
Change the protein in solution, later described for magnetic state under, the sampling hammerhead (200) spues except containing bead complexes
Solution, to complete the cleavage step (S21).
15. sample processing method according to claim 12, which is characterized in that the microscope carrier transport establishment (1) is included in
The upper layer microscope carrier shipping platform (111) of ground arrangement is penetrated through along short transverse (H) in at least partly described working region and lower layer carries
Platform shipping platform (112), first working region
(A), second working region (B) and the third working region (C) are sequentially arranged along microscope carrier moving direction (Z1)
On the upper layer microscope carrier shipping platform (111), the working region further includes the microscope carrier for storing multiple microscope carriers (2)
Initial storage area (D), the initial storage area of microscope carrier (D) be arranged in the upper layer microscope carrier shipping platform (111) and
The side of lower layer's microscope carrier shipping platform (112) and the region for being located at close first working region (A),
The microscope carrier driving mechanism includes microscope carrier driven in translation structure (12) and microscope carrier lifting driving structure (13), and the microscope carrier is flat
It moves driving structure (12) to be arranged on the upper layer microscope carrier shipping platform (111), and for driving the microscope carrier (2) described
Position is adjusted along the microscope carrier moving direction (Z1) on layer microscope carrier shipping platform (111), so that the microscope carrier (2) is moved to pair
The working region answered and successively execute first step (S1) to third step (S3), the microscope carrier goes up and down driving structure (13)
It is arranged on lower layer's microscope carrier shipping platform (112), and for driving the microscope carrier (2) to go up and down along short transverse (H), so that
Obtain the microscope carrier (2) toggle bit between the upper layer microscope carrier shipping platform (111) and lower layer's microscope carrier shipping platform (112)
It sets,
After the third step (S3), the sample processing method further includes microscope carrier return step (S4), utilizes the load
Platform goes up and down driving structure (13) and the microscope carrier (2) of the third working region (C) is transferred to the initial storage area of the microscope carrier
Domain (D),
Optionally, the working region further includes the first consumptive material storage area for sample dispensing in the first step (S1)
(E), in the first consumptive material storage area (E), which, which has, is used for the first lifting arm (6) setting
In the sampling hammerhead storage area (E1) of storage sampling hammerhead (200) and for storing the probe tube (300) for accommodating sample
Probe tube storage area (E2), first consumptive material storage area (E) be arranged on the upper layer microscope carrier shipping platform (111) and
Positioned at the top of the initial storage area of the microscope carrier (D), the first mechanical arm (6) is arranged in first consumptive material storage area
(E) it in and is arranged as, can be moved back and forth between first working region (A) and first consumptive material storage area (E),
Probe tube (300) are dispensed can be mounted in on the first working region (A) the sample container (3) on the microscope carrier (2) Nei
Interior sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810847784.XA CN109207341A (en) | 2018-07-27 | 2018-07-27 | sample processing device and sample processing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810847784.XA CN109207341A (en) | 2018-07-27 | 2018-07-27 | sample processing device and sample processing method |
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