CN105940305B - Extract separator and its working method - Google Patents
Extract separator and its working method Download PDFInfo
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- CN105940305B CN105940305B CN201480074414.7A CN201480074414A CN105940305B CN 105940305 B CN105940305 B CN 105940305B CN 201480074414 A CN201480074414 A CN 201480074414A CN 105940305 B CN105940305 B CN 105940305B
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- 239000000284 extract Substances 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims description 35
- 238000006243 chemical reaction Methods 0.000 claims abstract description 233
- 239000012530 fluid Substances 0.000 claims abstract description 146
- 238000005213 imbibition Methods 0.000 claims abstract description 141
- 239000007788 liquid Substances 0.000 claims abstract description 135
- 238000002347 injection Methods 0.000 claims abstract description 123
- 239000007924 injection Substances 0.000 claims abstract description 123
- 238000001179 sorption measurement Methods 0.000 claims abstract description 30
- 238000000926 separation method Methods 0.000 claims abstract description 23
- 238000013459 approach Methods 0.000 claims abstract description 20
- 239000002699 waste material Substances 0.000 claims description 36
- 238000002156 mixing Methods 0.000 claims description 35
- 238000004140 cleaning Methods 0.000 claims description 28
- 239000000243 solution Substances 0.000 claims description 22
- 238000010521 absorption reaction Methods 0.000 claims description 11
- 230000001360 synchronised effect Effects 0.000 claims description 8
- 238000006073 displacement reaction Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 239000000969 carrier Substances 0.000 claims description 3
- 230000032258 transport Effects 0.000 claims description 3
- 239000011324 bead Substances 0.000 description 21
- 230000033001 locomotion Effects 0.000 description 14
- 239000012535 impurity Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
Abstract
A kind of extract separator and its separation method, extract separator includes adsorbing mechanism (11), separator disk (12), liquid sucting mechanism (13), mechanism for filling liquid (14) and mobile mechanism (15), separator disk is rotatable annular mechanism, and multiple reaction cup positions (121) are equipped with along annular space, multiple imbibition positions and at least one fluid injection position are distributed with along its rotation approach in separator disk, adsorbing mechanism is also annular mechanism, adsorbing mechanism is equipped with multiple adsorptivity components (111) along annular, and a vacancy position (112) without adsorptivity component is at least set on the adsorbing mechanism, mobile mechanism's driving separator disk and adsorbing mechanism can rotate independently and stop, move vacancy position with adsorption capacity position is not needed.Since separator disk and adsorbing mechanism can rotate independently and stop, so that adsorbing mechanism can provide as needed adsorption capacity or not provide adsorption capacity.
Description
Technical field
This application involves purification or concentration technique fields, and in particular to extract separator and its working method.
Background technique
Extract separation method has a wide range of applications in purification or concentrated biological material sphere, generallys use Magneto separate skill
Art extracts components of interest from solution, the basic principle is that passing through one using the little particulate surface with magnetic material
Fixed processing allows to adsorb required substance, then by abandoning waste liquid after magnet absorption enrichment, and to the magnetic bead of enrichment
It is cleaned, further removes impurity and other unwanted substances, and biological substance interested is able to because being adsorbed on magnetic bead
Retain, in solution system required for being finally under certain condition discharged into the biological substance being enriched on magnetic bead.Such as Fig. 1 institute
It is shown as a basic Magneto separate process, listed step is respectively as follows:
Step 1: magnetic bead combines.Magnetic bead solution is added into the solution that needs purify, the sense for needing to purify or be concentrated is emerging
Interesting component is adsorbed onto magnetic bead surfaces.Strip is components of interest in figure, and bulk is impurity, and spherical is magnetic bead.
Step 2: magnetic bead absorption and cleaning separation.By externally-applied magnetic field magnetic bead is flocked together, and then convenient for cleaning
Remove impurity；
Step 3, elution release, solution required for the magnetic bead for completing to clean rejoins is by components of interest from magnetic bead
It releases, or directly carries out subsequent test.
Magnetic separating device is mainly used for completing step 2, generally includes for being loaded with the solution completing magnetic bead and combining
Injected in reaction vessel cleaning solution mechanism for filling liquid, for drawn from reaction vessel the liquid sucting mechanism of waste liquid, magnetic mechanism and
Mobile mechanism, mobile mechanism between mechanism for filling liquid and liquid sucting mechanism for moving reaction vessel.Reaction vessel completes cleaning
It can be removed after separation.
Currently used magnetic separating device includes multiple imbibition positions and multiple fluid injection positions, and magnetic is arranged in imbibition position
, and there is no magnetic field in fluid injection position, each imbibition position is put into the reaction vessel that reaction solution is housed, multiple imbibition machines
Structure executes parallel imbibition to multiple reaction vessels respectively and operates, and each reaction vessel is moved to corresponding note after the completion of imbibition
Liquid level is set, and then multiple mechanism for filling liquid execute parallel fluid injection to multiple reaction vessels respectively again and operate.
Summary of the invention
According to the application's in a first aspect, providing a kind of extract separator in one embodiment, comprising:
Separator disk, the separator disk are rotatable annular mechanism, and the separator disk is equipped with multiple arrangements along annular space
At at least one column for carrying the reaction cup position of reaction vessel, at least one suction is distributed with along its rotation approach in the separator disk
Liquid level is set and at least one fluid injection position；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism, which is equipped with one or more along annular, to be inhaled
The adsorptivity component of attached extract associativity carrier, and a sky without adsorptivity component is at least set on the adsorbing mechanism
It is vacant；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separator disk and adsorbing mechanism that can rotate independently and stopping, mobile mechanism's driving absorption
The vacancy position of mechanism follows the reaction cup displacement for not needing adsorption capacity dynamic.
According to the second aspect of the application, a kind of extract separator is provided in another embodiment, comprising:
Separator disk, the separator disk are rotatable annular mechanism, and the separator disk is equipped with multiple arrangements along annular space
At at least one column for carrying the reaction cup position of reaction vessel, at least one suction is distributed with along its rotation approach in the separator disk
Liquid level is set and at least one fluid injection position；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism, which is equipped with one or more along annular, to be inhaled
The adsorptivity component of attached extract associativity carrier, and a sky without adsorptivity component is at least set on the adsorbing mechanism
It is vacant；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separator disk and adsorbing mechanism that can rotate independently and stopping, at least on separator disk
When reaction vessel addition cleaning solution is in the mixing stage, the vacancy position of mobile mechanism's driving adsorbing mechanism follows the reaction vessel
Reaction cup bit synchronization is mobile.
According to the third aspect of the application, provide in one embodiment a kind of using magnetic separating device progress Magneto separate
Method, the magnetic separating device includes for carrying the annular release disk of reaction vessel and annular adsorbing mechanism, the separation
Disk is equipped with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one column along annular space, and the separator disk is along it
Rotation approach is distributed at least one imbibition position and at least one fluid injection position, the adsorbing mechanism along annular be equipped with one or
The adsorptivity component of multiple adsorbable extract associativity carriers, and one is at least arranged on the adsorbing mechanism without adsorptivity
The vacancy position of component；The described method includes: the separator disk drives reaction vessel to rotate and be to operate and stop, mobile mechanism
The vacancy position of adsorbing mechanism is driven to move with the position to extract associativity carrier offer adsorption capacity is not needed, in separator disk
Driving reaction vessel is during doing the stopping operated, and opposite direction stops at imbibition position or the reaction vessel execution of fluid injection position is predetermined
Operation.
According to the fourth aspect of the application, a kind of extract separator is provided in one embodiment, comprising:
Separator disk, the separator disk are rotatable annular mechanism, and the separator disk is equipped with multiple arrangements along annular space
At at least one column for carrying the reaction cup position of reaction vessel, at least one suction is distributed with along its rotation approach in the separator disk
Liquid level is set and at least one fluid injection position；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism, which is equipped with one or more along annular, to be inhaled
The adsorptivity component of attached extract associativity carrier, and a sky without adsorptivity component is at least set on the adsorbing mechanism
It is vacant；
Mobile mechanism, for driving separator disk and adsorbing mechanism that can rotate independently and stopping.
According to the 5th aspect of the application, a kind of extract separator is provided in one embodiment, comprising:
Separator disk, the separator disk are rotatable annular mechanism, and the separator disk is equipped with multiple arrangements along annular space
At the reaction cup position for carrying reaction vessel of at least one column and the circulation pipe position of the circulation pipe for carrying splendid attire waste liquid, institute
State circulation pipe position it is corresponding reaction cup position it is adjacent, at least one imbibition position is distributed with along its rotation approach in the separator disk
With at least one fluid injection position；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism, which is equipped with one or more along annular, to be inhaled
The adsorptivity component of attached extract associativity carrier, the position distribution of the adsorptivity component at least with the imbibition position on separator disk
It sets corresponding, and a vacancy position without adsorptivity component, the position distribution of vacancy position is at least set on the adsorbing mechanism
It is corresponding with fluid injection position；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separation disc spins and stopping.
According to the 6th aspect of the application, a kind of extract separator is provided in one embodiment, comprising:
Separator disk, the separator disk are rotatable annular mechanism, and the separator disk is equipped with multiple arrangements along annular space
At the reaction cup position for carrying reaction vessel of at least one column and the circulation pipe position of the circulation pipe for carrying splendid attire waste liquid, institute
State circulation pipe position it is corresponding reaction cup position it is adjacent, at least one imbibition position is distributed with along its rotation approach in the separator disk
Set, at least one fluid injection position and at least one mix position, the fluid injection position and mixing position it is adjacent；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism, which is equipped with one or more along annular, to be inhaled
The adsorptivity component of attached extract associativity carrier, the position distribution of the adsorptivity component at least with the imbibition position on separator disk
It sets corresponding, and a vacancy position without adsorptivity component, the position distribution of vacancy position is at least set on the adsorbing mechanism
It is at least corresponding with one of fluid injection position and mixing position；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separation disc spins and stopping.
Detailed description of the invention
Fig. 1 is a basic Magneto separate process；
Fig. 2 is the structural schematic diagram of extract separator in the embodiment of the present application one；
Fig. 3 is extract separation process figure in a duty cycle in the embodiment of the present application one；
Fig. 4 is the structural schematic diagram of circulation pipe in the embodiment of the present application two；
Fig. 5 is extract seperated schematic diagram in a duty cycle in the embodiment of the present application two；
Fig. 6 is the structural schematic diagram of extract separator in the embodiment of the present application three.
Specific embodiment
The embodiment of the present application is intended to provide a kind of extraction for carrying out circulation operation using single reaction vessel as processing unit
The reaction cup for being used to carry reaction vessel that at least one column constitute annular is distributed in object separator on extract separator
Multiple imbibition positions and at least one fluid injection position are distributed with along its rotation approach in position, reaction vessel rotate and be operate and
When stopping, predetermined operation corresponding with the operating position is executed to the reaction vessel for stopping at imbibition position or fluid injection position, but
Requirement of the different operations to extract associativity carrier adsorption power is different, some operations (or period) need absorption
Power, and some have not needed adsorption capacity.Have.Therefore in reaction vessel rotation or stopped process, mobile mechanism drives adsorption machine
Do not have on structure the vacancy position of adsorption capacity with do not need to extract associativity carrier provide adsorption capacity position and move.
Embodiment 1:
As shown in Fig. 2, extract separator 10 includes adsorbing mechanism 11, separator disk 12, liquid sucting mechanism 13, mechanism for filling liquid
14 and mobile mechanism 15.
Separator disk 12 is annular mechanism, the recyclable rotation of the annular mechanism, rotation approach can be it is round, oval,
Various polygons are irregular close-shaped, and separator disk is equipped with multiple for carrying the anti-of reaction vessel 122 along annular space
Cup position 121 is answered, multiple reaction cup positions 121 are arranged at least one column.At least one is also distributed with along its rotation approach in separator disk 12
Imbibition position and at least one fluid injection position.Alleged imbibition position is when separator disk is to operate and stop, to be imbibition behaviour
The position that the reaction vessel 122 of work is stopped, liquid sucting mechanism 13 execute imbibition operation to the reaction vessel for stopping at imbibition position.
Alleged fluid injection position be when separator disk is to operate and stop, for the position that is stopped of reaction vessel 122 for doing fluid injection operation,
Mechanism for filling liquid 14 executes fluid injection operation to the reaction vessel for stopping at fluid injection position.Imbibition position and fluid injection position can respectively have one
A or multiple, in a specific embodiment, at least partly imbibition position and fluid injection position is arranged alternately, imbibition position and fluid injection
Several reaction cup positions 121, the i.e. direction of rotation along separator disk 12, a several reaction cups of imbibition location interval are spaced between position
Fluid injection position is set behind position 121, imbibition position is set behind several reaction cup positions 121 of the fluid injection location interval.In some embodiments
The quantity of imbibition position is more than the quantity of fluid injection position, therefore along the direction of rotation of separator disk 12, the imbibition being arranged alternately before this
Then remaining imbibition position is arranged in position and fluid injection position.More thoroughly to draw waste liquid after the washing.Imbibition position
It can be arranged according to the specific demand of extract with the quantity of fluid injection position.In the present embodiment, multiple reaction cup positions 121 are arranged into one
Multiple reaction cup positions 121 can be arranged into two column or three column as needed, can had between each column by column in other embodiments
Respective imbibition position and fluid injection position can also share imbibition position and/or fluid injection position.
To prevent wicking structure that the waste liquid of absorption is transported to farther away probability and increase local and that improve cross contamination
The time of transhipment, in the preferred embodiment, separator disk 12 are additionally provided with multiple for carrying the circulation pipe for containing waste liquid along annular
Circulation pipe position 123, circulation pipe position 123 it is corresponding reaction cup position it is adjacent.Pipe circulate for containing waste liquid liquid sucting mechanism 13
The waste liquid drawn in corresponding reaction vessel is managed from circulation.
Liquid sucting mechanism 13 is used to execute imbibition operation in imbibition position, and liquid sucting mechanism 13, which is located at, in a kind of specific embodiment inhales
Liquid level sets top, and imbibition operation is executed by moving up and down；In other embodiments, liquid sucting mechanism 13 can not be located at imbibition
The top of position, and be located near imbibition position, fluid suction head is moved to the top of imbibition position by rotating, is then led to again
Up and down motion is crossed to execute imbibition operation.In the present embodiment, the quantity of liquid sucting mechanism 13 is identical as the quantity of imbibition position, each
Liquid sucting mechanism 13 has its corresponding imbibition position.
The effect of imbibition operation is that waste liquid is drawn from reaction vessel, is left by carrier-bound extract and associativity load
Body, waste liquid can be the waste liquid for being added and generating after reagent, be also possible to the waste liquid generated after cleaning solution is added.
Mechanism for filling liquid 14 is used to execute fluid injection operation in fluid injection position, and mechanism for filling liquid 14 is located at note in a kind of specific embodiment
Liquid level sets top, and fluid injection operation is executed by moving up and down；In other embodiments, mechanism for filling liquid 14 can not be located at fluid injection
The top of position, and be located near fluid injection position, filling head is moved to the top of fluid injection position by rotating, is then led to again
Up and down motion is crossed to execute fluid injection operation.It is that the quantity of mechanism for filling liquid 13 is identical as the quantity of fluid injection position, often in the present embodiment
A mechanism for filling liquid 14 has its corresponding fluid injection position.
The effect of fluid injection operation is that cleaning solution is injected into reaction vessel, to be adsorbed in reactor vessel wall or to combine
The impurity of property carrier cleans in solution.
Adsorbing mechanism 11 is also annular mechanism, and adsorbing mechanism 11 is equipped with multiple adsorbable extract associativity carriers along annular
Adsorptivity component 111, and a vacancy position 112 without adsorptivity component is at least set on adsorbing mechanism 11.Adsorptivity portion
Part 111 is corresponding with the reaction cup position 121 on separator disk 12, and i.e. vacating at least one reaction cup position does not have adsorptivity for vacancy position 112
Component.When extract associativity carrier is magnetic carrier, such as magnetic bead, adsorptivity component 111 are magnetic part, such as magnetic
Iron.In a kind of specific example, separator disk 12 and adsorbing mechanism 11 are the inside and outside annulus mechanism being nested, in specific example, absorption
Mechanism 11 can be in the outside of separator disk 12, can also be in the inside of separator disk 12, and the position with magnetic part, which corresponds to, to be divided
There is magnetic field, if having the magnetic for being adsorbed with extract in the reaction vessel on the reaction cup position on reaction cup position on separation disc 12
Property carrier, then magnetic carrier by magnetic part magneticaction, it is mobile to be simultaneously close to side of the reaction vessel close to magnetic part；It inhales
There is no the position of the vacancy position 112 of magnetic part to correspond to no magnetic field on the reaction cup position on separator disk 12 on random structure 11,
It, will not be by the magnetic force of magnetic part even if having the magnetic carrier for being adsorbed with extract in the reaction vessel on the reaction cup position
It acts on and assembles to the side of the close adsorbing mechanism 11 of reaction vessel.In another specific example, adsorbing mechanism 11 is located at separation
The bottom of disk 12, when the position with magnetic part corresponds to the reaction cup position on separator disk 12, magnetic carrier is by magnetic portion
The magneticaction of part, movement are simultaneously close to bottom of the reaction vessel close to magnetic part.In specific example, when separator disk 12 rotates way
When only one fluid injection position of diameter, the vacancy position 112 on adsorbing mechanism 11 can only be arranged one, and adsorptivity component 111 can also
With only one cricoid adsorptivity component；When 12 rotation approach of separator disk have multiple fluid injection positions (such as two or three) and
When multiple imbibition positions, it is meant that predetermined operation can be carried out to multiple reaction vessels simultaneously on separator disk 12, on adsorbing mechanism 11
Vacancy position 112 can also be correspondingly arranged multiple, and the spacer cup digit preferably between adjacent vacant omission 112 is equal to fluid injection position
Between spacer cup digit.To make multiple vacancy positions 112 while to follow multiple reaction cup displacements for not needing adsorption capacity dynamic.
Mobile mechanism 15 is for driving separator disk 12 and adsorbing mechanism that can rotate independently and stop, when on separator disk 12
When reaction vessel does not need to provide adsorption capacity, mobile mechanism 15 drives the vacancy position 112 of adsorbing mechanism 11 to follow the reaction vessel
Reaction cup displacement it is dynamic.In specific example, requirement of the different operations to extract associativity carrier adsorption power is different, needs sometimes
There is adsorption capacity, but do not need adsorption capacity sometimes, therefore the vacancy position 112 of the driving adsorbing mechanism 11 of mobile mechanism 15 follows and is not required to
Reaction cup bit synchronization where wanting the reaction vessel of adsorption capacity is mobile, so that the extract associativity carrier in reaction vessel
During this period not by adsorption force, extract associativity carrier suspends in the solution, be conducive to combine more extracts and
Clean the impurity on extract associativity carrier.In a kind of specific example, in reaction vessel rotation or stopped process, at least exist
When reaction vessel addition cleaning solution on separator disk is in the mixing stage, the vacancy position of mobile mechanism's driving adsorbing mechanism follows this
The reaction cup bit synchronization of reaction vessel is mobile, so that the extract associativity carrier in reaction vessel is after being added cleaning solution
The mixing stage not by adsorption force.
Drive the vacancy position 112 of adsorbing mechanism 11 that the reaction cup position of the reaction vessel is followed to start to synchronize in mobile mechanism 15
Before movement, this on the vacancy position 112 of possible adsorbing mechanism 11 and separator disk does not need the reaction vessel position of adsorption capacity
When different, mobile mechanism 15 first drives adsorbing mechanism to do the rotation relative to separator disk in such cases, rotates to vacancy position
This on separator disk does not need the reaction vessel position of adsorption capacity.
In a kind of specific example, mobile mechanism's control process is as follows:
Mobile mechanism's driving separator disk be do fluid injection operation and when stopping, mobile mechanism's driving adsorbing mechanism do relative to point
Separation disc rotation, so that vacancy position is rotated to position corresponding with fluid injection position, and from reaction vessel fluid injection to mixing during, moving machine
The vacancy position of structure driving adsorbing mechanism follows the reaction cup bit synchronization of the reaction vessel mobile.After the completion of mixing, mobile mechanism is again
Secondary driving adsorbing mechanism does the rotation relative to separator disk, by adsorptivity component transports to the reaction vessel for mixing operation with end
The corresponding position in reaction cup position at place, and follow the reaction cup bit synchronization mobile.
It mixes operation and is mixing position execution, mix position and be located in the rotation approach of separator disk, mixing position can be
Independently of the position of imbibition and fluid injection, can not also be located in the rotation approach of separator disk.In a kind of preferred embodiment, it will inhale
Liquid level at least one of is set as position is mixed, and liquid sucting mechanism executes the mixing behaviour after fluid injection by continuous suction operation
Make.
For being put into separator disk to each duty cycle for completing separation, the separation method packet of extract from by reaction vessel
Include cleaning step and separating step that sequence carries out.As shown in figure 3, cleaning step includes:
Step 30, the first imbibing step, separator disk drive reaction vessel rotation, when separator disk is to do imbibition operation and stop
When, liquid sucting mechanism executes imbibition operation to the reaction vessel in the reaction cup position for stopping at imbibition position；In the first imbibing step
In, the adsorptivity component of adsorbing mechanism is corresponding with the reaction cup position where carrying out the reaction vessel of imbibition, is in reaction vessel
Extract associativity carrier provides adsorption capacity, and mobile mechanism's driving adsorbing mechanism and separator disk synchronous rotary and stopping.
Step 31, fluid injection step is to make fluid injection operation when stopping when separator disk drives reaction vessel, and mechanism for filling liquid is to stopping
Only the reaction vessel in fluid injection position executes fluid injection operation.The adsorbing mechanism of mobile mechanism's driving simultaneously is done to be turned relative to separator disk
It is dynamic, so that vacancy position is rotated to position corresponding with fluid injection position, mechanism for filling liquid executes the reaction vessel for stopping at fluid injection position
Fluid injection operation.
Step 32, step is mixed, mobile mechanism drives separator disk and adsorbing mechanism synchronous rotary, reacts when separator disk drives
Container is to execute to the reaction vessel stopped in the reaction cup position for mixing position when making to mix operation and stop and mix operation,
After the completion of mixing, mobile mechanism's driving adsorbing mechanism does the rotation relative to separator disk, and adsorptivity component is made to rotate to and complete
The corresponding position in reaction cup position where the reaction vessel of mixing.
Step 33, separating step includes the second imbibing step, and separator disk drives reaction vessel rotation, the absorption of adsorbing mechanism
Property component it is corresponding with the reaction cup position where carrying out the reaction vessel of imbibition, mentioned for extract associativity carrier in reaction vessel
For adsorption capacity, and adsorbing mechanism is with separator disk synchronous rotary, and when separator disk is to do imbibition operation and stop, liquid sucting mechanism is to stopping
The only imbibition operation that the reaction vessel in the reaction cup position of imbibition position executes as separation purpose.
In other embodiments, adsorbing mechanism does not do the rotation relative to separator disk in fluid injection step, without the concern for
Whether vacancy position rotates to position corresponding with fluid injection position, and driving adsorbing mechanism is done relative to separator disk in mixing step
Rotation makes vacancy position rotate to position corresponding with position is mixed, and after the completion of mixing operation, mobile mechanism drives adsorbing mechanism again
The secondary rotation done relative to separator disk rotates to adsorptivity component corresponding with reaction cup position where the reaction vessel for completing to mix
Position.
Embodiment 2:
It is high due to requiring in the field the cross contamination test in some new fields, such as in foranalysis of nucleic acids, because
Common parts between needing strictly to avoid testing in this entire nucleic acid extraction process.It is mentioned using the separation of said extracted object separator
During taking object, liquid sucting mechanism needs operate different reaction vessels, to avoid sharing fluid suction head (also referred to as Tip),
Liquid sucting mechanism uses disposable fluid suction head, this requires the fluid suction head that liquid sucting mechanism constantly more renews, and causes the waste of fluid suction head.
To solve the problems, such as that disposable fluid suction head is frequently replaced, in a kind of specific example of the application, circulation pipe is designed as to carry
The structure of disposable fluid suction head, as shown in figure 4, circulation pipe 40 includes at least two chambers, a chamber 41 is for placing and the circulation
The disposable fluid suction head 42 of corresponding reaction vessel is managed, chamber 43 in addition is for holding waste liquid.It is adopted during extract Magneto separate
With the scheme of disposable fluid suction head imbibition are as follows:
Referring again to Fig. 2, a separator disk 12 is used to carry the reaction vessel 122 for needing Magneto separate, and matches therewith
The circulation pipe 124 used is covered, the effect of circulation pipe 124 is for carrying imbibition Tip and storage waste liquid；It is arranged in reaction vessel
Liquid sucting mechanism 13 and mechanism for filling liquid 14 above running track, wherein liquid sucting mechanism arranges 4 altogether, and mechanism for filling liquid arranges 2 altogether
A, liquid sucting mechanism needs to load the liquid that can just draw after Tip in reaction cup；The outside of Magneto separate disk arrange one can be with
The magnetic field ring of Magneto separate disk Concentric rotation, the fixation position of magnetic field ring are equipped with magnet for the magnetic bead in adsorption reaction cup.
Inside a specific embodiment, as shown in figure 5, being placed with 36 positions altogether on Magneto separate disk, it is respectively used to
Reaction cup and circulation pipe are carried, wherein reaction cup and sewer pipe interval enter Magneto separate disk.When working beginning, reaction cup and stream
By instrument, No. 36 position from Magneto separate disk is one in front and one in back put into Magneto separate disk to tube, spirals transmission then as Magneto separate
Into the corresponding outside magnetic field ring position of reaction cup has magnetic field in the process, so that magnetic bead cup is adsorbed onto reaction cup side；When
When circulation pipe is gone on Magneto separate disk as Magneto separate spirals below the liquid sucting mechanism of No. 9 i.e. the first rank in position, liquid sucting mechanism
The Tip loaded in circulation pipe is moved downward, is then lifted；Thereafter Magneto separate disk is drawn back a position, so that reaction cup is just
No. 9 position on into Magneto separate disk, liquid sucting mechanism decline are lifted after drawing the liquid in reaction cup；Then Magneto separate disk continues
Advance a position, so that circulation pipe is located at No. 9 position on Magneto separate disk, then liquid sucting mechanism moves downward discharge waste liquid simultaneously
Unload Tip.In the process, since interior removal waste fluid position is managed in circulation and the unloading position Tip is not consistent, it is therefore desirable to drained useless
Magneto separate disk is re-moved to the position of unloading Tip after liquid, naturally it is also possible to by the design for the pipe that circulates, so that two positions are protected
The step of holding unanimously, then not needing the movement of Magneto separate disk.So far Magneto separate the first rank imbibition movement is completed, in above process,
The holding consistent with the magnetic field ring of its outer ring of Magneto separate disk moves synchronously or stops, therefore reaction cup is constantly in magnetic field adsorbed state
Under.After completing the first rank imbibition movement, Magneto separate disk moves on 3 positions, so that reaction cup enters on Magneto separate disk the
Below No. 11 positions, that is, mechanism for filling liquid, during Magneto separate disk advances, relative motion occurs therewith and makes magnetic field ring for magnetic field ring
On the part in not no magnetic field correspond to reaction cup, mechanism for filling liquid injects cleaning under no magnetic field suction-operated into reaction cup at this time
Liquid, 3 positions of Magneto separate disk rollback make circulation pipe come back to the first rank imbibition position thereafter, and liquid sucting mechanism decline loads
Tip, then Magneto separate disk continues to retreat a position, so that reaction cup is located at below the first rank imbibition position, under liquid sucting mechanism
Drop in reaction cup cleaning solution and magnetic bead carry out suction mixing after lift, then Magneto separate disk moves on a position, makes
The pipe that must circulate, which is located at below liquid sucting mechanism, unloads Tip, and so far Magneto separate the first rank fluid injection and mixing movement are completed, in the process
Magneto separate disk is always maintained at magnetic field ring and moves synchronously and stop, therefore reaction cup is constantly in the state of no magnetic field absorption,
It is resuspended in cleaning solution convenient for magnetic bead.Magnetic field ring after mixing is completed to relatively rotate with Magneto separate disk so that at reaction cup again
In the state of having magnetic field, continue the magnetic field absorption of lower single order.Second-order Magneto separate imbibition and fluid injection mixing movement with it is above-mentioned
Process repeats, and then third rank then only carries out imbibition movement, acts without fluid injection, blots the liquid in reaction cup, fourth order
It acts and repeats with third rank, the residual liquid of bottom of a cup is further blotted in not fluid injection imbibition.So far, entire Magneto separate has acted
At.Then reaction cup and the pipe that circulates take reaction cup and circulation away by instrument as Magneto separate disk continues to move back into No. 36 positions
Pipe carries out subsequent operation.
Certain above process is designed for a specific extraction property, according to clean level required for extracting
Difference different and to residual liquid can increase or reduce the number of imbibition and fluid injection, or even increase injection last
Other reaction solution ingredients required for follow-up test, are all possible deformation schemes.
In above-described embodiment, separator disk, which rotates approach along it, can also only be arranged an imbibition position and a fluid injection position
It sets, for a reaction vessel, one duty cycle are as follows: reaction vessel is first transported to imbibition position and executes absorption waste liquid
Operation, is then transported to fluid injection position for reaction vessel again, cleaning solution is injected into reaction vessel, finally again turns reaction vessel
It transports to imbibition position and executes the operation for drawing waste liquid.
Embodiment 3:
Unlike the embodiments above is that separator disk is rotatable annular mechanism, and mobile mechanism is for driving separation to spiral
Turn and stops.Adsorbing mechanism is fixed non-rotary ring structure, and adsorbing mechanism is equipped with multiple adsorbable extract knots along annular
The adsorptivity component of conjunction property carrier, the position distribution of the adsorptivity component is at least corresponding with the imbibition position on separator disk,
And a vacancy position without adsorptivity component is at least set on the adsorbing mechanism, the position distribution of vacancy position and fluid injection position
It is corresponding at least one of position is mixed, when the position distribution of vacancy position is with fluid injection position and all corresponding mixing position,
Fluid injection position is adjacent with position is mixed.The separation process of extract is illustrated by taking Magneto separate as an example below.
As shown in fig. 6, a rotatable Magneto separate disk 60, is uniformly distributed multiple reaction cup positions above, needed for carrying
The reaction cup 61 for wanting Magneto separate has magnetic field structure, such as magnetic part 62 in the outer ring fixed position arrangement of Magneto separate disk, is used for
Magnetic bead in adsorption reaction cup, Magneto separate disk top are respectively disposed with the mechanism for filling liquid 63 and liquid sucting mechanism 64 that can be moved up and down.
Magnetic field structure further includes the vacancy position 65 of a not no magnetic part, and vacancy position 65 is corresponding with fluid injection position.Magneto separate process is opened
When the beginning, reaction cup is placed into Magneto separate disk, and the magnetic bead in reaction cup is adsorbed onto the one of reaction cup by the magnetic field outside Magneto separate disk
Side, following reaction cup rotate forward with Magneto separate disk, move to below liquid sucting mechanism, and liquid sucting mechanism moves downward disease and draws instead
The raffinate in cup is answered then to lift, Magneto separate disk drives reaction cup to move forward, and reaction cup reaches below mechanism for filling liquid, note
Liquid mechanism, which is moved downward, to be injected cleaning solution into reaction cup and is mixed, and is not had on the outside of the reaction cup below mechanism for filling liquid
Distribution of Magnetic Field, magnetic bead is re-suspended in cleaning solution at this time.Thereafter Magneto separate disk drives reaction cup to move forward under
Below one liquid sucting mechanism, magnetic bead is adsorbed onto the side of reaction cup by the magnet of Magneto separate disk outer ring again in the process, is inhaled
Liquid mechanism repeats the movement of upper single order, and movement thereafter is repeated up to the cleaning of completion last time and imbibition movement.Reaction cup is completed
It can be removed after Magneto separate.
Use above specific case is illustrated the application, is merely used to help understand the application not to limit
The application.For those of ordinary skill in the art, according to the thought of the application, above-mentioned specific embodiment can be become
Change.
Claims (28)
1. a kind of extract separator, characterized by comprising:
Separator disk, the separator disk are rotatable annular mechanism, the separator disk along annular space be equipped with it is multiple be arranged into
At least one imbibition position is distributed with along its rotation approach in the reaction cup position for being used to carry reaction vessel of few column, the separator disk
It sets and at least one fluid injection position；The quantity of the imbibition position is more than fluid injection position, and the separator disk is distributed along annular sequential
Have: the imbibition position and fluid injection position and remaining imbibition position being arranged alternately, it is described more thoroughly to draw waste liquid
Separator disk is additionally provided with multiple circulation pipe positions along annular, and the circulation pipe is for carrying disposable fluid suction head and holding waste liquid；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism is equipped with one or more adsorbable mention along annular
The adsorptivity component of object associativity carrier is taken, and a vacancy without adsorptivity component is at least set on the adsorbing mechanism
Position；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separator disk and adsorbing mechanism that can rotate independently and stopping, mobile mechanism drives adsorbing mechanism
Vacancy position follow the reaction cup displacement for not needing adsorption capacity dynamic.
2. a kind of extract separator, characterized by comprising:
Separator disk, the separator disk are rotatable annular mechanism, the separator disk along annular space be equipped with it is multiple be arranged into
At least one imbibition position is distributed with along its rotation approach in the reaction cup position for being used to carry reaction vessel of few column, the separator disk
It sets and at least one fluid injection position；The quantity of the imbibition position is more than fluid injection position, and the separator disk is distributed along annular sequential
Have: the imbibition position and fluid injection position and remaining imbibition position being arranged alternately, it is described more thoroughly to draw waste liquid
Separator disk is additionally provided with multiple circulation pipe positions along annular, and the circulation pipe is for carrying disposable fluid suction head and holding waste liquid；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism is equipped with one or more adsorbable mention along annular
The adsorptivity component of object associativity carrier is taken, and a vacancy without adsorptivity component is at least set on the adsorbing mechanism
Position；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separator disk and adsorbing mechanism that can rotate independently and stopping, the reaction at least on separator disk
When container addition cleaning solution is in the mixing stage, the vacancy position of mobile mechanism's driving adsorbing mechanism follows the reaction of the reaction vessel
Cup bit synchronization is mobile.
3. extract separator as claimed in claim 1 or 2, which is characterized in that mobile mechanism driving separator disk is
When doing fluid injection operation and stopping, the vacancy position of mobile mechanism's driving adsorbing mechanism rotates to position corresponding with fluid injection position, and
During from reaction vessel fluid injection to mixing, the vacancy position of mobile mechanism's driving adsorbing mechanism follows the reaction cup position of the reaction vessel
Synchronizing moving, after the completion of mixing, mobile mechanism drives the adsorptivity component transports of adsorbing mechanism to mix operation to end again
Reaction vessel where the corresponding position in reaction cup position, and follow the reaction cup bit synchronization mobile.
4. extract separator as claimed in claim 3, which is characterized in that the liquid sucting mechanism also passes through continuous suction
Operation executes the mixing operation after fluid injection.
5. extract separator as claimed in claim 1 or 2, which is characterized in that the separator disk and adsorbing mechanism are interior
The annulus mechanism or adsorbing mechanism being nested outside are located at the bottom of separator disk.
6. extract separator as claimed in claim 1 or 2, which is characterized in that the adsorptivity component is adsorbable magnetic
The magnetic part of property carrier.
7. extract separator as described in claim 1, which is characterized in that at least there are two fluid injection position, the absorption
The quantity of vacancy position in mechanism is equal to the quantity of fluid injection position, anti-in the position distribution and separator disk of the adsorptivity component
Answer cup position corresponding, the spacer cup digit between the spacer cup digit between the vacancy position and fluid injection position is identical.
8. extract separator as claimed in claim 1 or 2, which is characterized in that corresponding anti-in the circulation pipe position
Answer cup position adjacent.
9. extract separator as claimed in claim 3, which is characterized in that the corresponding reaction cup in the circulation pipe position
Position is adjacent.
10. extract separator as claimed in claim 4, which is characterized in that the corresponding reaction in the circulation pipe position
Cup position is adjacent.
11. extract separator as claimed in claim 5, which is characterized in that the corresponding reaction in the circulation pipe position
Cup position is adjacent.
12. extract separator as claimed in claim 6, which is characterized in that the corresponding reaction in the circulation pipe position
Cup position is adjacent.
13. extract separator as claimed in claim 7, which is characterized in that the corresponding reaction in the circulation pipe position
Cup position is adjacent.
14. a kind of method for carrying out Magneto separate using magnetic separating device, the magnetic separating device includes for carrying reaction vessel
Annular release disk and annular adsorbing mechanism, the separator disk along annular space be equipped with it is multiple be arranged at least one column for holding
The reaction cup position of reaction vessel is carried, at least one imbibition position and at least one note is distributed with along its rotation approach in the separator disk
Liquid level is set, and the quantity of the imbibition position is more than fluid injection position, and the separator disk is distributed with along annular sequential: the suction being arranged alternately
Liquid level set with fluid injection position and remaining imbibition position, more thoroughly to draw waste liquid, the separator disk is also set along annular
There are multiple circulation pipe positions, the circulation pipe is for carrying disposable fluid suction head and holding waste liquid；The adsorbing mechanism is along annular
Adsorptivity component equipped with one or more adsorbable extract associativity carriers, and one is at least arranged on the adsorbing mechanism
There is no the vacancy position of adsorptivity component；It is characterized in that the described method includes: the separator disk drives reaction vessel to rotate and be
Operate and stop, mobile mechanism driving adsorbing mechanism vacancy position with do not need to extract associativity carrier provide adsorption capacity
Position and move, separator disk drives reaction vessel be to do the stopping that operating during, opposite direction stops at imbibition position or fluid injection
The reaction vessel of position executes predetermined operation.
15. method as claimed in claim 14, which is characterized in that for being put into separator disk to completion separation from by reaction vessel
Each duty cycle, the method includes cleaning step and separating step that sequence carries out, cleaning step sequence includes:
First imbibing step, separator disk drive reaction vessel rotation, when separator disk is to do imbibition operation and stop, liquid sucting mechanism
Imbibition operation is executed to the reaction vessel in the reaction cup position for stopping at imbibition position；In the first imbibing step, adsorbing mechanism
Adsorptivity component it is corresponding with the reaction cup position where carrying out the reaction vessel of imbibition, be reaction vessel in extract associativity
Carrier provides adsorption capacity, and mobile mechanism's driving adsorbing mechanism and separator disk synchronous rotary and stopping；
Fluid injection step, when it is to make fluid injection operation and stop that separator disk, which drives reaction vessel, mobile mechanism's driving adsorbing mechanism is done
Relative to the rotation of separator disk, vacancy position is made to rotate to position corresponding with fluid injection position, mechanism for filling liquid is to stopping at fluid injection position
The reaction vessel set executes fluid injection operation；
The separating step includes:
Second of imbibing step, separator disk drive reaction vessel rotation, the adsorptivity component of adsorbing mechanism and will carry out imbibition
Reaction cup position where reaction vessel is corresponding, provides adsorption capacity, and adsorbing mechanism for extract associativity carrier in reaction vessel
With separator disk synchronous rotary, when separator disk is to do imbibition operation and stop, reaction of the liquid sucting mechanism to imbibition position is stopped at
The imbibition operation that container executes as separation purpose.
16. method as claimed in claim 15, which is characterized in that the cleaning step is after fluid injection step further include:
Step is mixed, mobile mechanism drives separator disk and adsorbing mechanism synchronous rotary, is to mix when separator disk drives reaction vessel
Even operation and when stopping, the reaction vessel stopped in the reaction cup position for mixing position is executed and mixes operation, after the completion of mixing,
Mobile mechanism's driving adsorbing mechanism does the rotation relative to separator disk, rotates to adsorptivity component and reacts appearance with complete to mix
The corresponding position in reaction cup position where device.
17. method as claimed in claim 14, which is characterized in that for being put into separator disk to completion separation from by reaction vessel
Each duty cycle, the method includes cleaning step and separating step that sequence carries out, cleaning step sequence includes:
First imbibing step, separator disk drive reaction vessel rotation, when separator disk is to do imbibition operation and stop, liquid sucting mechanism
Imbibition operation is executed to the reaction vessel in the reaction cup position for stopping at imbibition position；In the first imbibing step, adsorbing mechanism
Adsorptivity component it is corresponding with the reaction cup position where carrying out the reaction vessel of imbibition, be reaction vessel in extract associativity
Carrier provides adsorption capacity, and mobile mechanism's driving adsorbing mechanism and separator disk synchronous rotary and stopping；
Fluid injection step is to make fluid injection operation when stopping when separator disk drives reaction vessel, and mechanism for filling liquid is to stopping at fluid injection position
The reaction vessel set executes fluid injection operation；
Step is mixed, the reaction when separator disk drive reaction vessel is to make to mix operation and stop, to mixing position is stopped at
Container, which executes, mixes operation, mixes in operating process, and mobile mechanism's driving adsorbing mechanism does the rotation relative to separator disk, makes sky
Omission rotates to position corresponding with position is mixed, and after the completion of mixing operation, mobile mechanism's driving adsorbing mechanism is done relatively again
In the rotation of separator disk, adsorptivity component is made to rotate to position corresponding with reaction cup position where the reaction vessel for completing to mix；
The separating step includes:
Second of imbibing step, separator disk drive reaction vessel rotation, the adsorptivity component of adsorbing mechanism and will carry out imbibition
Reaction cup position where reaction vessel is corresponding, provides adsorption capacity, and adsorbing mechanism for extract associativity carrier in reaction vessel
With separator disk synchronous rotary, when separator disk is to do imbibition operation and stop, reaction of the liquid sucting mechanism to imbibition position is stopped at
The imbibition operation that reaction vessel in cup position executes as separation purpose.
18. the method as described in claim 16 or 17, which is characterized in that the mixing position is located at imbibition position, the suction
Liquid mechanism also operates the mixing after executing fluid injection by continuous suction and operates.
19. method as claimed in claim 18, which is characterized in that the separator disk is additionally provided with multiple for carrying stream along annular
The circulation pipe position of tube, the corresponding reaction cup position in the circulation pipe position are adjacent；First imbibing step and the second imbibing step
Respectively include:
By carry fluid suction head, with by the corresponding circulation pipe of reaction vessel for executing imbibition operation be transported to imbibition position, suction
Liquid mechanism loads fluid suction head from the circulation pipe for stopping at imbibition position；
The corresponding reaction vessel of circulation pipe is transported to imbibition position, is loaded with the liquid sucting mechanism of fluid suction head from stopping at imbibition
Waste liquid is drawn in the reaction vessel of position；
Imbibition position will be transported to the corresponding circulation pipe of reaction vessel for completing imbibition operation, liquid sucting mechanism is to stopping at imbibition
Waste liquid is discharged in the circulation pipe of position, then fluid suction head is unloaded in circulation pipe.
20. method as claimed in claim 19, which is characterized in that the mixing step includes:
By carry fluid suction head, with will execute the corresponding circulation pipe of reaction vessel for mixing operation and be transported to imbibition position, suction
Liquid mechanism loads fluid suction head from the circulation pipe for stopping at imbibition position；
The corresponding reaction vessel of circulation pipe is transported to imbibition position, is loaded with the liquid sucting mechanism of fluid suction head to stopping at imbibition
The reaction vessel of position executes suction and mixes operation；
Circulation pipe corresponding with the reaction vessel for completing mixing operation is transported to imbibition position, liquid sucting mechanism unloads fluid suction head
Into circulation pipe.
21. the method as described in any one of claim 15-17, which is characterized in that the cleaning step executes repeatedly, described
Second of imbibing step executes multiple.
22. method as claimed in claim 18, which is characterized in that the cleaning step executes repeatedly second of imbibition
Step executes multiple.
23. method as claimed in claim 19, which is characterized in that the cleaning step executes repeatedly second of imbibition
Step executes multiple.
24. method as claimed in claim 20, which is characterized in that the cleaning step executes repeatedly second of imbibition
Step executes multiple.
25. a kind of extract separator, characterized by comprising:
Separator disk, the separator disk are rotatable annular mechanism, the separator disk along annular space be equipped with it is multiple be arranged into
At least one imbibition position is distributed with along its rotation approach in the reaction cup position for being used to carry reaction vessel of few column, the separator disk
It sets and at least one fluid injection position；The quantity of the imbibition position is more than fluid injection position, and the separator disk is distributed along annular sequential
Have: the imbibition position and fluid injection position and remaining imbibition position being arranged alternately, it is described more thoroughly to draw waste liquid
Separator disk is additionally provided with multiple circulation pipe positions along annular, and the circulation pipe is for carrying disposable fluid suction head and holding waste liquid；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism is equipped with one or more adsorbable mention along annular
The adsorptivity component of object associativity carrier is taken, and a vacancy without adsorptivity component is at least set on the adsorbing mechanism
Position；
Mobile mechanism, for driving separator disk and adsorbing mechanism that can rotate independently and stopping.
26. a kind of extract separator, characterized by comprising:
Separator disk, the separator disk are rotatable annular mechanism, the separator disk along annular space be equipped with it is multiple be arranged into
The reaction cup position for carrying reaction vessel of few column and the circulation pipe position of the circulation pipe for carrying splendid attire waste liquid, the stream
The corresponding reaction cup position in tube position is adjacent, at least one imbibition position and extremely is distributed with along its rotation approach in the separator disk
A few fluid injection position；The quantity of the imbibition position is more than fluid injection position, and the separator disk is distributed with along annular sequential: alternately
The imbibition position and fluid injection position and remaining imbibition position of setting, more thoroughly to draw waste liquid,The separator disk edge
Annular is additionally provided with multiple circulation pipe positions, and the circulation pipe is for carrying disposable fluid suction head and holding waste liquid；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism is equipped with one or more adsorbable mention along annular
Take the adsorptivity component of object associativity carrier, the position distribution of the adsorptivity component at least with the imbibition position phase on separator disk
It is corresponding, and a vacancy position without adsorptivity component, the position distribution and note of vacancy position are at least set on the adsorbing mechanism
Liquid level is set corresponding；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separation disc spins and stopping.
27. a kind of extract separator, characterized by comprising:
Separator disk, the separator disk are rotatable annular mechanism, the separator disk along annular space be equipped with it is multiple be arranged into
The reaction cup position for carrying reaction vessel of few column and the circulation pipe position of the circulation pipe for carrying splendid attire waste liquid, the stream
The corresponding reaction cup position in tube position is adjacent, at least one imbibition position, extremely is distributed with along its rotation approach in the separator disk
A few fluid injection position and at least one mixing position, the fluid injection position are adjacent with position is mixed；The number of the imbibition position
Amount is more than fluid injection position, and the separator disk is distributed with along annular sequential: the imbibition position and fluid injection position being arranged alternately, and surplus
Remaining imbibition position, more thoroughly to draw waste liquid,The separator disk is additionally provided with multiple circulation pipe positions, the circulation along annular
Pipe is for carrying disposable fluid suction head and holding waste liquid；
Adsorbing mechanism, the adsorbing mechanism are annular mechanism, and the adsorbing mechanism is equipped with one or more adsorbable mention along annular
Take the adsorptivity component of object associativity carrier, the position distribution of the adsorptivity component at least with the imbibition position phase on separator disk
It is corresponding, and a vacancy position without adsorptivity component, the position distribution and note of vacancy position are at least set on the adsorbing mechanism
Liquid level at least one of is set and mixes position corresponding；
For executing the liquid sucting mechanism of imbibition operation in imbibition position；
For executing the mechanism for filling liquid of fluid injection operation in fluid injection position；
Mobile mechanism, for driving separation disc spins and stopping.
28. the extract separator as described in claim 26 or 27, which is characterized in that the adsorptivity component is adsorbable
The magnetic part of magnetic carrier, the adsorbing mechanism are to fix not rotational structure, inside and outside the separator disk and adsorbing mechanism composition
The mechanism or adsorbing mechanism being nested are located at the bottom of separator disk.
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| CN201910679522.1A CN110488029B (en) | 2014-06-17 | 2014-06-17 | Extract separating device and working method thereof |
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| PCT/CN2014/080126 WO2015192328A1 (en) | 2014-06-17 | 2014-06-17 | Extract isolation apparatus and method of operation thereof |
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| CN107377563B (en) * | 2017-08-24 | 2021-01-08 | 深圳市亚辉龙生物科技股份有限公司 | Magnetic separation cleaning device and working method thereof and liquid injection needle tube |
| CN108169501A (en) * | 2017-12-04 | 2018-06-15 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
| CN108296213A (en) * | 2017-12-26 | 2018-07-20 | 深圳德夏科技发展有限公司 | Board-washing machine |
| CN110865196B (en) * | 2018-08-27 | 2023-10-20 | 深圳迎凯生物科技有限公司 | Immunoassay analyzer |
| CN112296049B (en) * | 2019-07-26 | 2022-04-05 | 深圳迎凯生物科技有限公司 | Cleaning method |
| WO2021016733A1 (en) * | 2019-07-26 | 2021-02-04 | 深圳迎凯生物科技有限公司 | Cleaning method, cleaning apparatus, and immunity analyzer |
| CN112858660A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Magnetic separation cleaning system and imbibition needle subassembly, immunoassay appearance thereof |
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| CN110488029B (en) | 2024-01-16 |
| CN110488029A (en) | 2019-11-22 |
| WO2015192328A1 (en) | 2015-12-23 |
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