CN105940305A - Extract isolation apparatus and method of operation thereof - Google Patents
Extract isolation apparatus and method of operation thereof Download PDFInfo
- Publication number
- CN105940305A CN105940305A CN201480074414.7A CN201480074414A CN105940305A CN 105940305 A CN105940305 A CN 105940305A CN 201480074414 A CN201480074414 A CN 201480074414A CN 105940305 A CN105940305 A CN 105940305A
- Authority
- CN
- China
- Prior art keywords
- imbibition
- separator disk
- reaction vessel
- fluid injection
- adsorbing mechanism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Provided are an extract isolation apparatus and a method of operation thereof; the extract isolation apparatus comprises an adsorption mechanism (11), a separation disc (12), a pipetting mechanism (13), an injection mechanism (14), and a motion mechanism (15); the separation disc is a rotatable annular mechanism, and along same is provided a plurality of reaction cuvette positions (121) spaced at intervals; along the rotational path of the separation disc are distributed a plurality of pipetting positions and at least one injection position; the adsorption mechanism is also an annular mechanism; the adsorption mechanism is provided with a plurality of adsorptive components (111) along its perimeter, and said adsorption mechanism is at least provided with one vacant position (112) having no adsorptive component; the motion mechanism drives the separation disc and adsorption mechanism to rotate and stop independently, and the vacant position moves with the position requiring no adsorptive force. The separation disc and adsorption mechanism are driven to rotate and stop independently, such that the adsorptive mechanism can provide an adsorptive force or not provide an adsorptive force, according to requirements.
Description
Extract separator and its method of work technical field
The application is related to purification or concentration technique field, and in particular to extract separator and its method of work.
Background technology
Extract separation method has a wide range of applications in purification or concentrated biological material sphere, generally components of interest is extracted from solution using magnetic separation technique, its general principle is to utilize the little particulate surface with magnetic material it is adsorbed required material by certain processing, then waste liquid is abandoned after being enriched with by magnet adsorption, and the magnetic bead of enrichment is cleaned, further remove impurity and other unwanted materials, and biological substance interested is retained because adsorbing on magnetic bead, finally the biological substance being enriched on magnetic bead is discharged into required solution system under certain condition.It is as shown in Figure 1 a basic Magneto separate flow, listed step is respectively：
Step 1:Magnetic bead is combined.Magnetic bead solution is added into the solution for needing to purify, it would be desirable to which the components of interest of purification or concentration is adsorbed onto magnetic bead surfaces.Strip is components of interest in figure, and bulk is impurity, and spherical is magnetic bead.
Step 2:Magnetic bead is adsorbed and cleaning separation.By externally-applied magnetic field so that magnetic bead flocks together, and then it is easy to cleaning to go the removal of impurity；
Step 3, elution release, the solution required for the magnetic bead of completion cleaning is rejoined releases components of interest from magnetic bead, or directly carries out follow-up test.
Magnetic separating device is mainly used in completing step 2, generally include for the mechanism for filling liquid of injection cleaning fluid in the reaction vessel for completing the solution that magnetic bead is combined, liquid sucting mechanism, magnetic mechanism and travel mechanism for drawing waste liquid from reaction vessel is loaded with, travel mechanism to be used to move reaction vessel between mechanism for filling liquid and liquid sucting mechanism.Reaction vessel can be removed after completing cleaning separation.
Conventional magnetic separating device includes multiple imbibition positions and multiple fluid injection positions at present, in imbibition position, magnetic field is set, and there is no magnetic field in fluid injection position, each imbibition position is put into a reaction vessel that reaction solution is housed, multiple liquid sucting mechanisms perform parallel imbibition to multiple reaction vessels respectively and operated, each reaction vessel is moved to corresponding fluid injection position after the completion for the treatment of imbibition, and then multiple mechanism for filling liquid perform parallel fluid injection to multiple reaction vessels respectively again and operated.The content of the invention
According to the application's in a first aspect, providing a kind of extract separator in one embodiment, including：
Separator disk, the separator disk is rotatable annular mechanism, and the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, the separator disk
At least one imbibition position and at least one fluid injection position is distributed with along its rotation approach；Adsorbing mechanism, the adsorbing mechanism is annular mechanism, and the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without absorption ' 1 life part on the adsorbing mechanism；
Liquid sucting mechanism for performing imbibition operation in imbibition position；
Mechanism for filling liquid for performing fluid injection operation in fluid injection position；
Travel mechanism, for driving separator disk and adsorbing mechanism independently can to rotate and stop, the vacancy position of travel mechanism's driving adsorbing mechanism follows the reaction cup displacement for not needing absorption affinity to move.
According to the second aspect of the application, a kind of extract separator is provided in another embodiment, including：
Separator disk, the separator disk is rotatable annular mechanism, the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, and at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk；
Adsorbing mechanism, the adsorbing mechanism is annular mechanism, and the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without absorption ' 1 life part on the adsorbing mechanism；
Liquid sucting mechanism for performing imbibition operation in imbibition position；
Mechanism for filling liquid for performing fluid injection operation in fluid injection position；
Travel mechanism, for driving separator disk and adsorbing mechanism independently can to rotate and stop, when reaction vessel at least on separator disk adds cleaning fluid in the mixed hook stage, the vacancy position of travel mechanism's driving adsorbing mechanism follows the reaction cup bit synchronization of the reaction vessel to move.
The third aspect according to the application, a kind of method that use magnetic separating device carries out Magneto separate is provided in one embodiment, the magnetic separating device includes the annular release disk and annular adsorbing mechanism for being used to carry reaction vessel, the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk, the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism；Methods described includes：The separator disk drives reaction vessel to rotate and stop to operate, moved with the position of absorption affinity need not be provided to extract associativity carrier the vacancy position of travel mechanism's driving adsorbing mechanism, separator disk drives reaction vessel be to do the stopping that operating during, to performing scheduled operation to the reaction vessel for stopping at imbibition position or fluid injection position.
According to the fourth aspect of the application, a kind of extract separator is provided in one embodiment, including：
Separator disk, the separator disk is rotatable annular mechanism, and the separator disk is along annular space
Provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk；
Adsorbing mechanism, the adsorbing mechanism is annular mechanism, and the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without absorption ' 1 life part on the adsorbing mechanism；
Travel mechanism, for driving separator disk and adsorbing mechanism independently can to rotate and stop.
According to the 5th aspect of the application, a kind of extract separator is provided in one embodiment, including：
Separator disk, the separator disk is rotatable annular mechanism, the separator disk contains the circulation pipe position of the circulation pipe of waste liquid along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row and for carrying, the corresponding reaction cup position in the circulation pipe position is adjacent, and at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk；
Adsorbing mechanism, the adsorbing mechanism is annular mechanism, the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, the position distribution of the adsorptivity part is at least corresponding with the imbibition position on separator disk, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism, the position distribution of vacancy position is corresponding with fluid injection position；Liquid sucting mechanism for performing imbibition operation in imbibition position；
Mechanism for filling liquid for performing fluid injection operation in fluid injection position；
Travel mechanism, for driving separation disc spins and stopping.
According to the 6th aspect of the application, a kind of extract separator is provided in one embodiment, including：
Separator disk, the separator disk is rotatable annular mechanism, the separator disk contains the circulation pipe position of the circulation pipe of waste liquid along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row and for carrying, the corresponding reaction cup position in the circulation pipe position is adjacent, at least one imbibition position, at least one fluid injection position and at least one mixed hook position is distributed with along its rotation approach in the separator disk, and the fluid injection position is adjacent with mixed hook position；
Adsorbing mechanism, the adsorbing mechanism is annular mechanism, the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, the position distribution of the adsorptivity part is at least corresponding with the imbibition position on separator disk, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism, the position distribution of vacancy position is at least corresponding with one of fluid injection position and mixing position；
Liquid sucting mechanism for performing imbibition operation in imbibition position；
Mechanism for filling liquid for performing fluid injection operation in fluid injection position；
Travel mechanism, for driving separation disc spins and stopping.Brief description of the drawings
Fig. 1 is a basic Magneto separate flow；
Fig. 2 is the structural representation of extract separator in the embodiment of the present application one；
Fig. 3 is extract separation process figure in a work period in the embodiment of the present application one；Fig. 4 is the structural representation of circulation pipe in the embodiment of the present application two；
Fig. 5 is extract separation schematic diagram in a work period in the embodiment of the present application two；Fig. 6 is the structural representation of extract separator in the embodiment of the present application three.
Embodiment
The embodiment of the present application is intended to provide a kind of extract separator for carrying out circulation operation by processing unit of single reaction vessel, the reaction cup position for being used to carry reaction vessel that at least one row constitute annular is distributed with extract separator, multiple imbibition positions and at least one fluid injection position are distributed with along its rotation approach, when reaction vessel rotates and stopped to operate, scheduled operation corresponding with the operating position is performed to the reaction vessel for stopping at imbibition position or fluid injection position, but requirement of the different operations to extract associativity carrier adsorption power is different, some operations（Or the period）Absorption affinity is needed, and some need not have absorption affinity.Have.Therefore in reaction vessel rotation or stopped process, the vacancy position for not having absorption affinity on travel mechanism's driving adsorbing mechanism is moved with that need not provide absorption affinity position to extract associativity carrier.Embodiment 1:
As shown in Fig. 2 extract separator 10 includes adsorbing mechanism 11, separator disk 12, liquid sucting mechanism 13, mechanism for filling liquid 14 and travel mechanism 15.
Separator disk 12 is annular mechanism, the annular mechanism is recyclable to be rotated, it rotates approach Ke Yi Shi Round shape, Tuo Round shapes, various polygons or irregular close-shaped, separator disk is along annular space provided with multiple reaction cup positions 121 for being used to carry reaction vessel 1 22, and multiple reaction cup positions 1 21 are arranged into an at least row.At least one imbibition position and at least one fluid injection position is also distributed with along its rotation approach in separator disk 12.Alleged imbibition position is that, when separator disk stops to operate, to do the position that the reaction vessel 122 of imbibition operation is stopped, the reaction vessel that 13 pairs of liquid sucting mechanism stops at imbibition position performs imbibition operation.Alleged fluid injection position is that, when separator disk stops to operate, to do the position that the reaction vessel 122 of fluid injection operation is stopped, the reaction vessel that 14 pairs of mechanism for filling liquid stops at fluid injection position performs fluid injection operation.Imbibition position and fluid injection position can respectively have one or more, in a kind of specific embodiment, at least part imbibition position and fluid injection position are arranged alternately, if being spaced thousand reaction cups position 1 21 between imbibition position and fluid injection position, i.e. along the direction of rotation of separator disk 12
Fluid injection position is set behind some reaction cup positions 121 of one imbibition location interval, and the fluid injection location interval sets imbibition position behind some reaction cup positions 121.The quantity of imbibition position is more than the quantity of fluid injection position in some embodiments, therefore along the direction of rotation of separator disk 12, then the imbibition position and fluid injection position being arranged alternately before this set remaining imbibition position.More thoroughly to draw waste liquid after the washing.The quantity of imbibition position and fluid injection position can be set according to the specific demand of extract.In the present embodiment, multiple reaction cup positions 121 are arranged into a row, in other embodiments, multiple reaction cups position 121 can be arranged into two row or three row as needed, can there are respective imbibition position and fluid injection position between each row, imbibition position and/or fluid injection position can also be shared.
To prevent wicking structure from the place that the waste liquid of absorption is transported to farther out being improved to the probability of cross pollution and the time of increase transhipment, in preferred embodiment, separator disk 12 is additionally provided with multiple circulation pipe positions 123 for being used to carry the circulation pipe for containing waste liquid along annular, and the corresponding reaction cup position in circulation pipe position 123 is adjacent.Circulation pipe is used to contain the waste liquid that waste liquid liquid sucting mechanism 13 is drawn from the corresponding reaction vessel of circulation pipe.
Liquid sucting mechanism 13 is used to perform liquid sucting mechanism 13 in imbibition operation, a kind of specific embodiment in imbibition position and above imbibition position, imbibition operation is performed by moving up and down；In other embodiments, liquid sucting mechanism 13 can not be located at the top of imbibition position, and positioned at the vicinity of imbibition position, fluid suction head is moved to the top of imbibition position by rotating, and then perform imbibition operation by moving up and down again.In the present embodiment, the quantity of liquid sucting mechanism 13 is identical with the quantity of imbibition position, and each liquid sucting mechanism 13 has its corresponding imbibition position.
The effect of imbibition operation is that waste liquid is drawn from reaction vessel, is left by carrier-bound extract and associativity carrier, and waste liquid can be the waste liquid for adding the waste liquid produced after reagent or being produced after addition cleaning fluid.
Mechanism for filling liquid 14 is used to perform mechanism for filling liquid 14 in fluid injection operation, a kind of specific embodiment in fluid injection position and above fluid injection position, fluid injection operation is performed by moving up and down；In other embodiments, mechanism for filling liquid 14 can not be located at the top of fluid injection position, and positioned at the vicinity of fluid injection position, filling head is moved to the top of fluid injection position by rotating, and then perform fluid injection operation by moving up and down again.It is that the quantity of mechanism for filling liquid 13 is identical with the quantity of fluid injection position, each mechanism for filling liquid 14 has its corresponding fluid injection position in the present embodiment.
The effect of fluid injection operation is that cleaning fluid is injected into reaction vessel, to adsorb in reactor vessel wall or the impurity of associativity carrier is cleaned in solution.
Adsorbing mechanism 11 is also annular mechanism, and adsorbing mechanism 11 is provided with the adsorptivity part 111 of multiple adsorbable extract associativity carriers along annular, and does not have on adsorbing mechanism 11 at least provided with one
The vacancy position 112 of adsorptivity part.Adsorptivity part 111 is corresponding with the reaction cup position 121 on separator disk 12, and at least one reaction cup position is vacated in vacancy position 112 does not have adsorptivity part.When extract associativity carrier is magnetic carrier, such as magnetic bead, adsorptivity part 111 is magnetic part, such as magnet.In a kind of instantiation, separator disk 12 and adsorbing mechanism 11 are the inside and outside Round ring mechanisms that are nested, in instantiation, adsorbing mechanism 11 can be in the outside of separator disk 12, can also be in the inner side of separator disk 12, position with magnetic part, which corresponds on the reaction cup position on separator disk 12, has magnetic field, if with the magnetic carrier for being adsorbed with extract in reaction vessel on reaction cup position, then magnetic carrier is moved by the magneticaction of magnetic part and is simultaneously close to reaction vessel close to the side of magnetic part；There is no the upper no magnetic field in reaction cup position that the position of the vacancy position 112 of magnetic part corresponds on separator disk 12 on adsorbing mechanism 11, even if with the magnetic carrier for being adsorbed with extract in the reaction vessel on reaction cup position, will not also be assembled by the magneticaction of magnetic part to the side of the close adsorbing mechanism 11 of reaction vessel.In another instantiation, adsorbing mechanism 11 is located at the bottom of separator disk 12, when the position with magnetic part corresponds to the reaction cup position on separator disk 12, magnetic carrier is moved by the magneticaction of magnetic part and is simultaneously close to reaction vessel close to the bottom of magnetic part.In instantiation, when separator disk 12 rotates approach only one of which fluid injection position, the vacancy position 112 on adsorbing mechanism 11 can only set one, adsorptivity part 111 can also only one of which ring-type adsorptivity part；When the rotation approach of separator disk 12 has multiple fluid injection positions（For example two or three）During with multiple imbibition positions, mean that scheduled operation can be carried out to multiple reaction vessels simultaneously on separator disk 12, vacancy position 112 on adsorbing mechanism 11 can also be correspondingly arranged multiple, and spacer cup digit preferably between adjacent vacant omission 112 is equal to the spacer cup digit between fluid injection position.To make multiple vacancy positions 112 while following multiple reaction cup displacements for not needing absorption affinity to move.
Travel mechanism 15 is used to drive separator disk 12 and adsorbing mechanism independently can rotate and stop, when the reaction vessel on separator disk 12 need not provide absorption affinity, the vacancy position 112 of the driving adsorbing mechanism 11 of travel mechanism 15 follows the reaction cup displacement of the reaction vessel to move.In instantiation, requirement of the different operations to extract associativity carrier adsorption power is different, sometimes for there is absorption affinity, but absorption affinity is not needed sometimes, therefore the vacancy position 112 of the driving of travel mechanism 15 adsorbing mechanism 11 follows the reaction cup bit synchronization movement where the reaction vessel for not needing absorption affinity, so that the extract associativity carrier in reaction vessel is not acted on by absorption affinity during this period, extract associativity carrier suspends in the solution, is conducive to the impurity for combining more extracts and cleaning on extract associativity carrier.In a kind of instantiation, in reaction vessel rotation or stopped process, when reaction vessel at least on separator disk adds cleaning fluid in the mixed hook stage, the vacancy position of travel mechanism's driving adsorbing mechanism follows the reaction cup bit synchronization of the reaction vessel to move, so that carrying in reaction vessel
Mixed hook stage of the thing associativity carrier after cleaning fluid is added is taken not acted on by absorption affinity.Before travel mechanism 15 drives the vacancy position 112 of adsorbing mechanism 11 to follow the reaction cup position of the reaction vessel to start synchronizing moving, when the vacancy position 1 12 of possible adsorbing mechanism 11 does not need the reaction vessel position of absorption affinity different from being somebody's turn to do on separator disk, travel mechanism 15 first drives adsorbing mechanism to do the rotation relative to separator disk in such cases, vacancy position is rotated on separator disk and should not need the reaction vessel position of absorption affinity.
In a kind of instantiation, travel mechanism's control process is as follows：
When travel mechanism's driving separator disk stops to do fluid injection operation, travel mechanism's driving adsorbing mechanism is done to be rotated relative to separator disk, vacancy position is set to rotate to position corresponding with fluid injection position, and from reaction vessel fluid injection to mixed hook during, travel mechanism driving adsorbing mechanism vacancy position follow the reaction cup bit synchronization of the reaction vessel to move.It is mixed hook after the completion of, travel mechanism drives adsorbing mechanism to do the rotation relative to separator disk again, will adsorb ' component transports arrive with terminate the mixed reaction vessel for hooking operation where the corresponding position in reaction cup position, and follow reaction cup bit synchronization movement.
Mix operation and mixing the execution of hook position, mix and hook position in the rotation approach of separator disk, mixing position can be independently of the position of imbibition and fluid injection, can not also be in the rotation approach of separator disk.In a kind of preferred embodiment, using at least one in imbibition position as mixed hook position, liquid sucting mechanism operates the mixed hook operation performed after fluid injection by continuous suction.
For being put into separator disk to each work period for completing separation from by reaction vessel, the separation method of extract includes cleaning step and separating step that order is carried out.As shown in figure 3, cleaning step includes：
Step 30, the first imbibing step, separator disk drives reaction vessel rotation, when stopping when separator disk to do imbibition operation, and the reaction vessel in reaction cup position of the liquid sucting mechanism to stopping at imbibition position performs imbibition operation；In the first imbibing step, the adsorptivity part of adsorbing mechanism is corresponding with by the reaction cup position where the reaction vessel for carrying out imbibition, absorption affinity, and travel mechanism's driving adsorbing mechanism and separator disk synchronous rotary and stopping are provided for extract associativity carrier in reaction vessel.
Step 31, fluid injection step, when separator disk, which drives reaction vessel, to be stopped to make fluid injection operation, mechanism for filling liquid performs fluid injection operation to the reaction vessel for stopping at fluid injection position.The adsorbing mechanism of travel mechanism's driving simultaneously does the rotation relative to separator disk, vacancy position is rotated to position corresponding with fluid injection position, and mechanism for filling liquid performs fluid injection operation to the reaction vessel for stopping at fluid injection position.
Step 32, it is mixed to hook step, travel mechanism drives separator disk and adsorbing mechanism synchronous rotary, when separator disk, which drives reaction vessel, to be stopped to make mixed hook operation, to stopping at, the reaction vessel execution in the mixed reaction cup position for hooking position is mixed to hook operation, after the completion of mixed hook, travel mechanism's driving adsorbing mechanism
Do the rotation relative to separator disk, make absorption ' ti parts rotate to complete the mixed reaction vessel hooked where the corresponding position in reaction cup position.
Step 33, separating step includes the second imbibing step, separator disk drives reaction vessel rotation, the adsorptivity part of adsorbing mechanism is corresponding with by the reaction cup position where the reaction vessel for carrying out imbibition, absorption affinity is provided for extract associativity carrier in reaction vessel, and adsorbing mechanism is with separator disk synchronous rotary, when stopping when separator disk to do imbibition operation, the reaction vessel in reaction cup position of the liquid sucting mechanism to stopping at imbibition position performs to be operated for the imbibition of separation purpose.
In other embodiments, adsorbing mechanism does not do the rotation relative to separator disk in fluid injection step, position corresponding with fluid injection position whether is rotated to without the concern for vacancy position, driving adsorbing mechanism does the rotation relative to separator disk in mixed hook step, vacancy position is set to rotate to position corresponding with mixed hook position, after the completion of mixed hook operation, travel mechanism's driving adsorbing mechanism does the rotation relative to separator disk again, adsorptivity part is rotated to position corresponding with completing the mixed reaction vessel place reaction cup position hooked.Embodiment 2:
In some new fields, such as foranalysis of nucleic acids, need strictly to avoid common parts between test due to requiring high to the cross pollution test in the field, therefore in whole nucleic acid extraction flow.During using said extracted thing separator separating extractive, liquid sucting mechanism needs to operate different reaction vessels, to avoid sharing fluid suction head（Also referred to as T i p heads）, liquid sucting mechanism is using disposable fluid suction head, and this requires the fluid suction head that liquid sucting mechanism constantly more renews, and causes the waste of fluid suction head.To solve the problem of disposable fluid suction head is frequently changed, in a kind of instantiation of the application, circulation pipe is designed as that the structure of disposable fluid suction head can be carried, as shown in Fig. 4, the pipe 40 that circulates includes at least two chambers, one chamber 41 is used for the disposable fluid suction head 42 for placing reaction vessel corresponding with the circulation pipe, and chamber 43 in addition is used to hold waste liquid.Used during extract Magneto separate the scheme of disposable fluid suction head imbibition for：
Referring again to Fig. 2 ,-individual separator disk 12, which is used to carry, needs the reaction vessel 122 of Magneto separate, and the matched circulation pipe 124 used, and the effect of circulation pipe 124 is for carrying imbibition Ti p and storage waste liquid；Liquid sucting mechanism 13 and mechanism for filling liquid 14 above reaction vessel running orbit are arranged in, wherein liquid sucting mechanism arranges 4 altogether, and mechanism for filling liquid arranges 1 altogether, and liquid sucting mechanism needs just draw the liquid in reaction cup after loading T i p；Magnetic field ring that can be with Magneto separate disk Concentric rotation is arranged in the outside of Magneto separate disk, the fixed position of magnetic field ring is provided with the magnetic bead that magnet is used in adsorption reaction cup.
Inside a specific embodiment, as shown in Fig. 5,36 positions are placed with altogether on Magneto separate disk, carrying reaction cup is respectively used to and circulation is managed, wherein reaction cup and sewer pipe interval enter Magneto separate disk.When working beginning, reaction cup is with circulation pipe by instrument from the 36th on Magneto separate disk
Number position is one in front and one in back put into Magneto separate disk, and progressive then as Magneto separate disc spins, the corresponding outside magnetic field ring position of reaction cup has magnetic field in the process so that magnetic bead cup is adsorbed onto reaction cup side；When the pipe that circulates is gone on Magneto separate disk as Magneto separate spirals below the liquid sucting mechanism of No. 9 i.e. the first rank in position, liquid sucting mechanism moves downward the Tip loaded in circulation pipe, then lifts；Thereafter Magneto separate disk is drawn back a position so that reaction cup is lifted just into No. 9 position on Magneto separate disk, liquid sucting mechanism after declining the liquid drawn in reaction cup；Then Magneto separate disk moves on a position so that circulation pipe is located at No. 9 position on Magneto separate disk, and then liquid sucting mechanism moves downward discharge waste liquid and unloads Ti p.In the process, because removal waste fluid position and unloading Ti p positions are not consistent in circulation pipe, therefore Magneto separate disk after drained waste liquid is needed to be re-moved to the position for unloading Tip, can certainly be by the design of the pipe that circulates, so that two positions are consistent, then the step of Magneto separate disk is moved is not needed.So far the first rank of Magneto separate imbibition action is completed, in above process, and the holding consistent with the magnetic field ring of its outer ring of Magneto separate disk is synchronized with the movement or stopped, therefore reaction cup is constantly under the adsorbed state of magnetic field.Complete after the first rank imbibition action, Magneto separate disk moves on 3 positions, so that reaction cup is entered on Magneto separate disk below o.11 position i.e. mechanism for filling liquid, during the advance of Magneto separate disk, therewith relative motion occurs for magnetic field ring so that not having the part correspondence reaction cup in magnetic field on the ring of magnetic field, now mechanism for filling liquid injects cleaning fluid into reaction cup under no magnetic field suction-operated, thereafter 3 positions of Magneto separate disk rollback cause circulation pipe to come back to the first rank imbibition position, liquid sucting mechanism, which declines, loads Tip, then Magneto separate disk continues to retreat a position, so that reaction cup is located at below the first rank imbibition position, liquid sucting mechanism declines to carry out lifting after suction mixes hook to the cleaning fluid in reaction cup and magnetic bead, then Magneto separate disk moves on a position, so that circulation pipe is located at unloading Tip below liquid sucting mechanism, so far Magneto separate the first rank fluid injection and the action of mixed hook are completed, Magneto separate disk is always maintained at being synchronized with the movement and stopped with magnetic field ring in the process, therefore reaction cup is constantly in the state of no magnetic field absorption, it is easy to magnetic bead to be resuspended in cleaning fluid.Magnetic field ring is relatively rotated with Magneto separate disk again after the mixed hook of completion causes reaction cup in the state of having magnetic field, the magnetic field absorption of single order under continuing.The Magneto separate imbibition and fluid injection mixing action of second-order are repeated with said process, then the 3rd rank then only carries out imbibition action, is acted without fluid injection, blots the liquid in reaction cup, fourth order is acted with the 3rd rank and repeated, and the residual liquid at glass bottom is further blotted in not fluid injection imbibition.So far, whole Magneto separate action is completed.Then reaction cup and the pipe that circulates take reaction cup away by instrument and circulation pipe carry out follow-up operation as Magneto separate disk continues to move back into No. 36 positions.
Certain said process is designed for a specific extraction property, the difference of clean level according to required for extraction and to the difference of residual liquid, it can increase or reduce imbibition and the number of times of fluid injection, all it is possible deformation program even in other reaction solution compositions required for last increase injection follow-up test.
In above-described embodiment, separator disk rotates approach along it can also only set an imbibition position and a fluid injection position, and for a reaction vessel, the one work period is：Reaction vessel is first transported to the operation that imbibition position performs absorption waste liquid, reaction vessel is then transported to fluid injection position again
Put, cleaning fluid is injected into reaction vessel, reaction vessel is finally transported to the operation that imbibition position performs absorption waste liquid again.Embodiment 3:
Unlike the embodiments above is that separator disk is rotatable annular mechanism, and travel mechanism is used to drive separation disc spins and stopping.Adsorbing mechanism is fixed non-rotary loop configuration, adsorbing mechanism is provided with the adsorptivity part of multiple adsorbable extract associativity carriers along annular, the position distribution of the adsorptivity part is at least corresponding with the imbibition position on separator disk, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism, the position distribution of vacancy position is corresponding with least one in fluid injection position and mixing position, when the position distribution of vacancy position is with fluid injection position and all corresponding mixing position, fluid injection position is adjacent with position is mixed.The separation process to extract by taking Magneto separate as an example is illustrated below.
As shown in Figure 6, one rotatable Magneto separate disk 60, the multiple reaction cup positions of distribution are hooked above, the reaction cup 61 of Magneto separate is needed for carrying, there is magnetic field structure in the outer ring fixed position arrangement of Magneto separate disk, such as magnetic part 62, for the magnetic bead in adsorption reaction cup, the mechanism for filling liquid 63 and liquid sucting mechanism 64 that can be moved up and down are respectively disposed with above Magneto separate disk.Magnetic field structure also includes a vacancy position 65 without magnetic part, and vacancy position 65 is corresponding with fluid injection position.When Magneto separate process starts, reaction cup is placed into Magneto separate disk, magnetic bead in reaction cup is adsorbed onto the side of reaction cup by the magnetic field outside Magneto separate disk, following reaction cup rotates forward with Magneto separate disk, motion Magneto separate disk drives reaction cup to move forward, reaction cup is reached below mechanism for filling liquid, mechanism for filling liquid, which is moved downward, to be injected cleaning fluid into reaction cup and is mixed, without Distribution of Magnetic Field on the outside of reaction cup below mechanism for filling liquid, now magnetic bead is re-suspended in cleaning fluid.Thereafter Magneto separate disk drives reaction cup to move forward to below next liquid sucting mechanism, magnetic bead is adsorbed onto the side of reaction cup by the magnet of Magneto separate disk outer ring again in the process, liquid sucting mechanism repeats the action of upper single order, and action thereafter is repeated up to the cleaning of completion last time and imbibition action.Reaction cup can be removed after completing Magneto separate.Use above specific case is illustrated to the application, is only intended to help and is understood the application not to limit the application.For those of ordinary skill in the art, according to the thought of the application, above-mentioned embodiment can be changed.
Claims (21)
- Claim1. a kind of extract separator, it is characterised in that including：Separator disk, the separator disk is rotatable annular mechanism, the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, and at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk；Adsorbing mechanism, the adsorbing mechanism is annular mechanism, and the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without absorption ' 1 life part on the adsorbing mechanism；Liquid sucting mechanism for performing imbibition operation in imbibition position；Mechanism for filling liquid for performing fluid injection operation in fluid injection position；Travel mechanism, for driving separator disk and adsorbing mechanism independently can to rotate and stop, the vacancy position of travel mechanism's driving adsorbing mechanism follows the reaction cup displacement for not needing absorption affinity to move.2. a kind of extract separator, it is characterised in that including：Separator disk, the separator disk is rotatable annular mechanism, the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, and at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk；Adsorbing mechanism, the adsorbing mechanism is annular mechanism, and the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without absorption ' 1 life part on the adsorbing mechanism；Liquid sucting mechanism for performing imbibition operation in imbibition position；Mechanism for filling liquid for performing fluid injection operation in fluid injection position；Travel mechanism, for driving separator disk and adsorbing mechanism independently can to rotate and stop, when reaction vessel at least on separator disk adds cleaning fluid in the mixed hook stage, the vacancy position of travel mechanism's driving adsorbing mechanism follows the reaction cup bit synchronization of the reaction vessel to move.3. extract separator as claimed in claim 1 or 2, it is characterized in that, when travel mechanism's driving separator disk stops to do fluid injection operation, the vacancy position of travel mechanism's driving adsorbing mechanism rotates to position corresponding with fluid injection position, and from reaction vessel fluid injection to mixing during, the vacancy position of travel mechanism's driving adsorbing mechanism follows the reaction cup bit synchronization of the reaction vessel to move, after the completion of mixed hook, travel mechanism drive again the adsorptivity component transports of adsorbing mechanism to terminate the mixed reaction vessel for hooking operation where the corresponding position in reaction cup position, and follow the reaction cup bit synchronization to move.4. extract separator as claimed in claim 3, it is characterised in that the imbibition Mechanism also operates the mixed hook operation performed after fluid injection by continuous suction.5. extract separator as claimed in claim 1 or 2, it is characterised in that the separator disk and adsorbing mechanism are located at the bottom of separator disk for the inside and outside Round ring mechanisms that are nested, or adsorbing mechanism.6. extract separator as claimed in claim 1 or 2, it is characterised in that the adsorptivity part is the magnetic part of adsorbable magnetic carrier.7. the extract separator as any one of claim 1-6, it is characterised in that the quantity of the imbibition position is more than fluid injection position, the separator disk is distributed with along annular sequential：The imbibition position and fluid injection position being arranged alternately, and imbibition position.8. extract separator as claimed in claim 7, it is characterized in that, at least two fluid injection positions, the quantity of vacancy position on the adsorbing mechanism is equal to the quantity of fluid injection position, the position distribution of the adsorptivity part is corresponding with the reaction cup position on separator disk, and the spacer cup digit between spacer cup digit and fluid injection position between the vacancy position is identical.9. the extract separator as any one of claim 1-8, it is characterized in that, the separator disk is additionally provided with multiple circulation pipe positions for being used to carry the circulation pipe for containing waste liquid along annular, and the corresponding reaction cup position in the circulation pipe position is adjacent.The method that a kind of 1 0. use magnetic separating devices carry out Magneto separate, the magnetic separating device includes the annular release disk and annular adsorbing mechanism for being used to carry reaction vessel, the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk, the adsorbing mechanism gives birth to part along absorption ' 1 of the annular provided with one or more adsorbable extract associativity carriers, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism；It is characterized in that methods described includes：The separator disk drives reaction vessel to rotate and stop to operate, moved with the position of absorption affinity need not be provided to extract associativity carrier the vacancy position of travel mechanism's driving adsorbing mechanism, separator disk drives reaction vessel be to do the stopping that operating during, to performing scheduled operation to the reaction vessel for stopping at imbibition position or fluid injection position.1 1. method as described in claim 10, it is characterised in that for being put into separator disk to each work period for completing separation from by reaction vessel, methods described includes the cleaning step and separating step that order is carried out, and the cleaning step order includes：First imbibing step, separator disk drives reaction vessel rotation, when stopping when separator disk to do imbibition operation, and the reaction vessel in reaction cup position of the liquid sucting mechanism to stopping at imbibition position performs imbibition operation；In the first imbibing step, the adsorptivity part of adsorbing mechanism is with will carry out the anti-of imbibition The reaction cup position correspondence where container is answered, absorption affinity, and travel mechanism's driving adsorbing mechanism and separator disk synchronous rotary and stopping are provided for extract associativity carrier in reaction vessel；Fluid injection step, when separator disk, which drives reaction vessel, to be stopped to make fluid injection operation, travel mechanism's driving adsorbing mechanism does the rotation relative to separator disk, vacancy position is rotated to position corresponding with fluid injection position, and mechanism for filling liquid performs fluid injection operation to the reaction vessel for stopping at fluid injection position；The separating step includes：Second of imbibing step, separator disk drives reaction vessel rotation, the adsorptivity part of adsorbing mechanism is corresponding with by the reaction cup position where the reaction vessel for carrying out imbibition, absorption affinity is provided for extract associativity carrier in reaction vessel, and adsorbing mechanism is with separator disk synchronous rotary, when stopping when separator disk to do imbibition operation, liquid sucting mechanism is performed to the reaction vessel for stopping at imbibition position to be operated for the imbibition of separation purpose.12. the method as described in claim 11, it is characterised in that the cleaning step also includes after fluid injection step：Mix step, travel mechanism drives separator disk and adsorbing mechanism synchronous rotary, when separator disk, which drives reaction vessel, to be stopped to make mixed hook operation, to stopping at, the reaction vessel execution in the mixed reaction cup position for hooking position is mixed to hook operation, after the completion of mixed hook, travel mechanism's driving adsorbing mechanism does the rotation relative to separator disk, adsorptivity part is rotated to position corresponding with completing the mixed reaction vessel place reaction cup position hooked.13. method as claimed in claim 10, it is characterised in that for being put into separator disk to each work period for completing separation from by reaction vessel, methods described includes the cleaning step and separating step that order is carried out, and the cleaning step order includes：First imbibing step, separator disk drives reaction vessel rotation, when stopping when separator disk to do imbibition operation, and the reaction vessel in reaction cup position of the liquid sucting mechanism to stopping at imbibition position performs imbibition operation；In the first imbibing step, the adsorptivity part of adsorbing mechanism is corresponding with by the reaction cup position where the reaction vessel for carrying out imbibition, absorption affinity, and travel mechanism's driving adsorbing mechanism and separator disk synchronous rotary and stopping are provided for extract associativity carrier in reaction vessel；Fluid injection step, when separator disk, which drives reaction vessel, to be stopped to make fluid injection operation, mechanism for filling liquid performs fluid injection operation to the reaction vessel for stopping at fluid injection position；Mix step, when separator disk, which drives reaction vessel, to be stopped to make mixed hook operation, mixed hook operation is performed to stopping at the mixed reaction vessel for hooking position, it is mixed to hook in operating process, travel mechanism's driving adsorbing mechanism does the rotation relative to separator disk, vacancy position is rotated to position corresponding with mixed hook position, mix after the completion of hook operation, travel mechanism's driving adsorbing mechanism does the rotation relative to separator disk again Adsorptivity part is set to rotate to position corresponding with completing the mixed reaction vessel place reaction cup position hooked；The separating step includes：Second of imbibing step, separator disk drives reaction vessel rotation, the adsorptivity part of adsorbing mechanism is corresponding with by the reaction cup position where the reaction vessel for carrying out imbibition, absorption affinity is provided for extract associativity carrier in reaction vessel, and adsorbing mechanism is with separator disk synchronous rotary, when stopping when separator disk to do imbibition operation, the reaction vessel in reaction cup position of the liquid sucting mechanism to stopping at imbibition position performs to be operated for the imbibition of separation purpose.14. the method as described in claim 12 or 13, it is characterised in that the mixed hook position is located at imbibition position, the liquid sucting mechanism also operates the mixing operation performed after fluid injection by continuous suction.15. method as claimed in claim 14, it is characterised in that the separator disk is additionally provided with multiple circulation pipe positions for being used to carry circulation pipe along annular, the corresponding reaction cup position in the circulation pipe position is adjacent；First imbibing step and the second imbibing step include respectively：By carry fluid suction head, with by perform imbibition operation reaction vessel it is corresponding circulation pipe be transported to imbibition position, liquid sucting mechanism loads fluid suction head from the circulation pipe for stopping at imbibition position；The corresponding reaction vessel of circulation pipe is transported to imbibition position, the liquid sucting mechanism of fluid suction head is loaded with and draws waste liquid from the reaction vessel for stopping at imbibition position；Circulation pipe corresponding with the reaction vessel for completing imbibition operation is transported to imbibition position, liquid sucting mechanism discharges waste liquid into the circulation pipe for stopping at imbibition position, and then fluid suction head is unloaded in circulation pipe.16. the method as described in claim 15, it is characterised in that the mixed hook step includes：By carry fluid suction head, circulation pipe corresponding with the mixed reaction vessel for hooking operation will be performed be transported to imbibition position, liquid sucting mechanism loads fluid suction head from the circulation pipe for stopping at imbibition position；The corresponding reaction vessel of circulation pipe is transported to imbibition position, the liquid sucting mechanism for being loaded with fluid suction head performs suction mixing operation to the reaction vessel for stopping at imbibition position；Circulation pipe corresponding with completing the mixed reaction vessel for hooking operation is transported to imbibition position, fluid suction head is unloaded in circulation pipe by liquid sucting mechanism.17. the method as any one of claim 11 to 16, it is characterised in that the cleaning step is performed repeatedly, and second of imbibing step performs multiple.18. a kind of extract separator, it is characterised in that including：Separator disk, the separator disk is rotatable annular mechanism, and the separator disk is along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row, the separator disk At least one imbibition position and at least one fluid injection position is distributed with along its rotation approach；Adsorbing mechanism, the adsorbing mechanism is annular mechanism, and the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, and at least provided with a vacancy position without absorption ' 1 life part on the adsorbing mechanism；Travel mechanism, for driving separator disk and adsorbing mechanism independently can to rotate and stop.A kind of 1 9. extract separators, it is characterised in that including：Separator disk, the separator disk is rotatable annular mechanism, the separator disk contains the circulation pipe position of the circulation pipe of waste liquid along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row and for carrying, the corresponding reaction cup position in the circulation pipe position is adjacent, and at least one imbibition position and at least one fluid injection position is distributed with along its rotation approach in the separator disk；Adsorbing mechanism, the adsorbing mechanism is annular mechanism, the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, the position distribution of the adsorptivity part is at least corresponding with the imbibition position on separator disk, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism, the position distribution of vacancy position is corresponding with fluid injection position；Liquid sucting mechanism for performing imbibition operation in imbibition position；Mechanism for filling liquid for performing fluid injection operation in fluid injection position；Travel mechanism, for driving separation disc spins and stopping.20. a kind of extract separator, it is characterised in that including：Separator disk, the separator disk is rotatable annular mechanism, the separator disk contains the circulation pipe position of the circulation pipe of waste liquid along annular space provided with multiple reaction cup positions for being used to carry reaction vessel for being arranged at least one row and for carrying, the corresponding reaction cup position in the circulation pipe position is adjacent, at least one imbibition position, at least one fluid injection position and at least one mixed hook position is distributed with along its rotation approach in the separator disk, and the fluid injection position is adjacent with mixed hook position；Adsorbing mechanism, the adsorbing mechanism is annular mechanism, the adsorbing mechanism is provided with the adsorptivity part of one or more adsorbable extract associativity carriers along annular, the position distribution of the adsorptivity part is at least corresponding with the imbibition position on separator disk, and at least provided with a vacancy position without adsorptivity part on the adsorbing mechanism, the position distribution of vacancy position is corresponding with least one of fluid injection position and mixing position；Liquid sucting mechanism for performing imbibition operation in imbibition position；Mechanism for filling liquid for performing fluid injection operation in fluid injection position；Travel mechanism, for driving separation disc spins and stopping.21. the extract separator as any one of claim 1 8-20, it is characterized in that, the adsorptivity part is the magnetic part of adsorbable magnetic carrier, the adsorbing mechanism is fixed not rotational structure, the mechanism being nested inside and outside separator disk and the adsorbing mechanism composition, or adsorbing mechanism are located at the bottom of separator disk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910679522.1A CN110488029B (en) | 2014-06-17 | 2014-06-17 | Extract separating device and working method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2014/080126 WO2015192328A1 (en) | 2014-06-17 | 2014-06-17 | Extract isolation apparatus and method of operation thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910679522.1A Division CN110488029B (en) | 2014-06-17 | 2014-06-17 | Extract separating device and working method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105940305A true CN105940305A (en) | 2016-09-14 |
| CN105940305B CN105940305B (en) | 2019-08-27 |
Family
ID=54934683
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910679522.1A Active CN110488029B (en) | 2014-06-17 | 2014-06-17 | Extract separating device and working method thereof |
| CN201480074414.7A Active CN105940305B (en) | 2014-06-17 | 2014-06-17 | Extract separator and its working method |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910679522.1A Active CN110488029B (en) | 2014-06-17 | 2014-06-17 | Extract separating device and working method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (2) | CN110488029B (en) |
| WO (1) | WO2015192328A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108169501A (en) * | 2017-12-04 | 2018-06-15 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
| CN108296213A (en) * | 2017-12-26 | 2018-07-20 | 深圳德夏科技发展有限公司 | Board-washing machine |
| CN110865196A (en) * | 2018-08-27 | 2020-03-06 | 深圳迎凯生物科技有限公司 | Immunoassay analyzer |
| WO2021016733A1 (en) * | 2019-07-26 | 2021-02-04 | 深圳迎凯生物科技有限公司 | Cleaning method, cleaning apparatus, and immunity analyzer |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107377563B (en) * | 2017-08-24 | 2021-01-08 | 深圳市亚辉龙生物科技股份有限公司 | Magnetic separation cleaning device and working method thereof and liquid injection needle tube |
| CN112296049B (en) * | 2019-07-26 | 2022-04-05 | 深圳迎凯生物科技有限公司 | Cleaning method |
| CN112858660A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Magnetic separation cleaning system and imbibition needle subassembly, immunoassay appearance thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0422867A (en) * | 1990-05-17 | 1992-01-27 | Toshiba Corp | Immune analysis system |
| EP0889328A1 (en) * | 1996-10-24 | 1999-01-07 | Japan Tectron Instruments Corporation | Automatic immunological analyzer |
| US20070092403A1 (en) * | 2005-10-21 | 2007-04-26 | Alan Wirbisky | Compact apparatus, compositions and methods for purifying nucleic acids |
| JP2009115752A (en) * | 2007-11-09 | 2009-05-28 | Fujirebio Inc | Separating apparatus |
| CN102279275A (en) * | 2011-06-22 | 2011-12-14 | 深圳市国赛生物技术有限公司 | Integrated apparatus for magnetic stirring, cleaning and separation |
| CN103599898A (en) * | 2013-10-29 | 2014-02-26 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer magnetic bead washing device |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL94212A0 (en) * | 1989-07-24 | 1991-01-31 | Tri Tech Partners And Triton B | Automated analytical apparatus and method |
| US20020070173A1 (en) * | 2000-12-08 | 2002-06-13 | Promega Corporation, Madison, Wisconsin | Apparatus and method for use in magnetic separation of magnetically attractable particles in a liquid |
| JP3996416B2 (en) * | 2001-09-21 | 2007-10-24 | Ｊｕｋｉ株式会社 | B / F separation method in nucleic acid hybridization |
| EP1508618B1 (en) * | 2003-08-19 | 2012-07-25 | Kurashiki Boseki Kabushiki Kaisha | Extracting apparatus |
| US20070244314A1 (en) * | 2004-05-18 | 2007-10-18 | Fujifilm Corporation | Method For Extracting Nucleic Acid And Nucleic Acid-Extracting Apparatus |
| JP2006006258A (en) * | 2004-06-29 | 2006-01-12 | Fuji Photo Film Co Ltd | Method for extracting nucleic acid and apparatus for extracting nucleic acid |
| CN101190439B (en) * | 2006-11-28 | 2011-07-20 | 深圳迈瑞生物医疗电子股份有限公司 | Automatic cleaning device and method |
| CN101539584B (en) * | 2008-03-18 | 2012-06-27 | 北京源德生物医学工程有限公司 | Reaction disc assembly |
| KR101025135B1 (en) * | 2008-04-09 | 2011-03-31 | (주)바이오니아 | The automatic purification apparatus, multi well plate kit and isolation method of nucleic acid from biological samples |
| CN101726616B (en) * | 2008-10-31 | 2014-07-16 | 深圳迈瑞生物医疗电子股份有限公司 | Automatic analytic device and working method thereof |
| EP2192186B1 (en) * | 2008-11-28 | 2016-03-09 | F. Hoffmann-La Roche AG | System and method for the automated extraction of nucleic acids |
| CN102221626B (en) * | 2010-04-14 | 2015-04-22 | 深圳迈瑞生物医疗电子股份有限公司 | Automatic biochemistry analyzer and working method thereof |
| WO2012130107A1 (en) * | 2011-03-25 | 2012-10-04 | 深圳迈瑞生物医疗电子股份有限公司 | Apparatus for automatic analysis and sample analysis method thereof |
| CN102565439B (en) * | 2011-12-29 | 2013-07-24 | 长春迪瑞医疗科技股份有限公司 | Incubation part device for full-automatic chemiluminescence immunity analyzer and control method for incubation part device |
| CN202631343U (en) * | 2012-04-17 | 2012-12-26 | 深圳迈瑞生物医疗电子股份有限公司 | Blending device and automatic sample analyzer |
| CN203310835U (en) * | 2013-04-16 | 2013-11-27 | 深圳迈瑞生物医疗电子股份有限公司 | Magnetic separation device, blending mechanism and immunoassay instrument |
| CN203426088U (en) * | 2013-08-22 | 2014-02-12 | 钟伟明 | Magnetic bead washing device |
| CN103760373B (en) * | 2014-02-21 | 2015-06-10 | 中国科学院苏州生物医学工程技术研究所 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
-
2014
- 2014-06-17 CN CN201910679522.1A patent/CN110488029B/en active Active
- 2014-06-17 WO PCT/CN2014/080126 patent/WO2015192328A1/en active Application Filing
- 2014-06-17 CN CN201480074414.7A patent/CN105940305B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0422867A (en) * | 1990-05-17 | 1992-01-27 | Toshiba Corp | Immune analysis system |
| EP0889328A1 (en) * | 1996-10-24 | 1999-01-07 | Japan Tectron Instruments Corporation | Automatic immunological analyzer |
| US20070092403A1 (en) * | 2005-10-21 | 2007-04-26 | Alan Wirbisky | Compact apparatus, compositions and methods for purifying nucleic acids |
| JP2009115752A (en) * | 2007-11-09 | 2009-05-28 | Fujirebio Inc | Separating apparatus |
| CN102279275A (en) * | 2011-06-22 | 2011-12-14 | 深圳市国赛生物技术有限公司 | Integrated apparatus for magnetic stirring, cleaning and separation |
| CN103599898A (en) * | 2013-10-29 | 2014-02-26 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer magnetic bead washing device |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108169501A (en) * | 2017-12-04 | 2018-06-15 | 珠海迪尔生物工程有限公司 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
| CN108296213A (en) * | 2017-12-26 | 2018-07-20 | 深圳德夏科技发展有限公司 | Board-washing machine |
| CN110865196A (en) * | 2018-08-27 | 2020-03-06 | 深圳迎凯生物科技有限公司 | Immunoassay analyzer |
| CN110865196B (en) * | 2018-08-27 | 2023-10-20 | 深圳迎凯生物科技有限公司 | Immunoassay analyzer |
| WO2021016733A1 (en) * | 2019-07-26 | 2021-02-04 | 深圳迎凯生物科技有限公司 | Cleaning method, cleaning apparatus, and immunity analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015192328A1 (en) | 2015-12-23 |
| CN110488029B (en) | 2024-01-16 |
| CN105940305B (en) | 2019-08-27 |
| CN110488029A (en) | 2019-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105940305A (en) | Extract isolation apparatus and method of operation thereof | |
| CN105940094A (en) | Nucleic acid extraction apparatus and method of operation thereof | |
| CN102656465B (en) | Sample processing device, sample processing method, and reaction container used in these device and method | |
| CN1161608C (en) | Magnetic material attracting/releasing control method making use of a pipe the device and various types of analyzer using the method | |
| US20140087370A1 (en) | Sample treatment device, sample treatment method, and reaction container for use therein | |
| CN102002461B (en) | IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device and method | |
| JP5623060B2 (en) | Method and apparatus for separating components bonded to magnetic particles by applying centrifugal force | |
| CN101747397A (en) | System and method for the automated extraction of nucleic acids | |
| CN207181272U (en) | Magnetic bead cleans separator and immunity analysis instrument automatically | |
| CN1174515A (en) | Centrifuge reagent delivery system | |
| CN103760373B (en) | Cleaning device in fully-automatic chemiluminescence immunity analyzer | |
| WO2013155966A1 (en) | High-speed biochemical analyzer | |
| CN106754345A (en) | Nucleic acid-extracting apparatus | |
| JP2010115647A5 (en) | ||
| KR101700624B1 (en) | Preparation appratus and operation method thereof | |
| CN102586225A (en) | Method for separating target molecules by controlling magnetic beads | |
| CN103990403B (en) | A kind of filtration integrated processing mechanism that is mixed of sample | |
| KR20170120782A (en) | Extraction and purification apparatus | |
| CN112881367A (en) | Cleaning module, sample analyzer with same and cleaning method | |
| CN1134545C (en) | Automatic DNA purificating apparatus | |
| CN107884591A (en) | Automatic cleaning separator | |
| CN107058292A (en) | A kind of method that paramagnetic particle method isolates and purifies DNA | |
| CN205501280U (en) | Magnetic bead separating device | |
| CN106434336A (en) | Magnetic bead separation apparatus used for nucleic acid extraction | |
| CN110819627A (en) | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |