CN108169501A - A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument - Google Patents
A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument Download PDFInfo
- Publication number
- CN108169501A CN108169501A CN201711256025.8A CN201711256025A CN108169501A CN 108169501 A CN108169501 A CN 108169501A CN 201711256025 A CN201711256025 A CN 201711256025A CN 108169501 A CN108169501 A CN 108169501A
- Authority
- CN
- China
- Prior art keywords
- detection
- fluorescence analysis
- exempts
- analysis instrument
- quantitative fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 19
- 238000012921 fluorescence analysis Methods 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 65
- 239000012085 test solution Substances 0.000 claims abstract description 22
- 238000005213 imbibition Methods 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims abstract description 18
- 230000033001 locomotion Effects 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims description 18
- 239000000758 substrate Substances 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 7
- 229910052724 xenon Inorganic materials 0.000 claims description 7
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 7
- 230000002459 sustained effect Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims 1
- 239000011324 bead Substances 0.000 abstract description 14
- 239000007788 liquid Substances 0.000 abstract description 11
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010070834 Sensitisation Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
Abstract
The invention discloses a kind of magnetic enzymes to exempt from quantitative fluorescence analysis instrument, including bottom plate, if the identical pipetting device of stem structure is placed on the bottom plate, the pipetting device includes bearing support, it is provided on the bearing support and moves forward and backward component, described move forward and backward is provided with board rack on component, up and down motion component is provided on the bearing support, mounting blocks are set on the up and down motion component, imbibition piston is fixed on the mounting blocks, the rear end of the pipetting device is provided with detection drive component, and testing agency is provided on the detection drive component;Board rack moves to liquid relief station, imbibition piston puts the magnetic bead test solution picked up into the test solution slot of board, then board rack is moved to by testing agency backward, so that test solution slot is located at below testing agency, detection is automatically performed using testing agency, entire reaction process is more intelligent, reduces artificial participation, improves the precision and efficiency of detection.
Description
Technical field
The present invention relates to chemical apparatuses apparatus fields more particularly to a kind of magnetic enzyme to exempt from quantitative fluorescence analysis instrument.
Background technology
It is using luminescent system and immunoassay method, to serum, blood plasma or other bodies that magnetic enzyme, which exempts from quantitative fluorescence analysis instrument,
Various analytes in liquid carry out the instrument of quantitative detection.Reagent lath is built-in test solution and magnetic bead, and magnetic bead is to utilize high score
The Magnetic solid phases particulate of sub- materials synthesis certain particle size size, magnetic bead is coated with as carrier has each of specific affinity
Kind immunologic active material makes its sensitization be mixed the magnetic bead in liquid to be checked and reagent lath, then for Immune-magnetic Particles
Liquid to be detected is observed using detecting system, obtains testing result.Common fluorescence reaction instrument is exactly by magnetic bead on the market
Colour developing observation or micro- detection are carried out after test solution and liquid to be detected mixing, it can not be automatically by liquid to be detected and magnetic bead test solution
It is mixed, previous step still needs manually to be operated so that detection rates and accuracy of detection are all very low.
Invention content
In order to overcome the above-mentioned deficiencies of the prior art, the present invention provides one kind to carry out liquid to be detected and magnetic bead test solution automatically
Mixing, observation is detected to solution after mixing automatically, the magnetic enzyme for substantially increasing processing efficiency and precision exempts from fluorescent quantitation
Analyzer.
To achieve these goals, the technical solution adopted by the present invention is:
A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument, including bottom plate, if being placed with the identical liquid relief of stem structure on the bottom plate
Mechanism, the pipetting device includes bearing support, is provided on the bearing support and moves forward and backward component, described to move forward and backward on component
Board rack is provided with, up and down motion component is provided on the bearing support, mounting blocks are set on the up and down motion component,
Imbibition piston is fixed on the mounting blocks, the lower end of the imbibition piston is provided with suction nozzle seat, is provided in the suction nozzle seat
The suction nozzle being connect with imbibition piston, the rear end of the pipetting device is provided with detection drive component, described to detect on drive component
It is provided with testing agency.
As being further improved for said program, electric rotating machine, the master of the electric rotating machine are provided on the bearing support
Axis is hinged with connecting rod by transmission mechanism, and the end of the connecting rod is provided with magnet, and the electric rotating machine drives connecting rod around hinged
Point rotation so that magnet is separate or close to board rack.
As being further improved for said program, the transmission mechanism is gear set, and gear set includes being set in electric rotating
First gear on machine, hinged second gear and the transmission gear of the connection first gear and second gear with connecting rod.
As being further improved for said program, the up and down motion component includes vertical slide rail, in the vertical slide rail
Vertical slipper is provided with, the mounting blocks are fixed on vertical slipper, and the mounting blocks are connected with the first driving motor.
As being further improved for said program, the component that moves forward and backward includes horizontal slide rail, on the horizontal slide rail
Equipped with horizontal slider, the board rack is fixed on the horizontal slider, and the board rack is connected with the second driving
Motor.
As being further improved for said program, testing agency's movement is arranged on the installation on detection drive component
, detection hole is provided in the mounting base, light transmission channel, the light transmission channel are connected with below the detection hole
The xenon lamp of transmitting light is inside provided with, the photomultiplier for receiving light, the photomultiplier transit are provided with by the detection hole
The front end of pipe is provided with detection lens subassembly, and the mounting base is fixedly arranged above detection scanner, the detection scanner position
In the top of detection hole.
As being further improved for said program, the detection lens subassembly includes lens mount, is arranged on from front to back
Convex lens, the first optical filtering and acquisition photodiode in lens mount.
As being further improved for said program, light source detection channel, the light are also associated with below the detection hole
Front and rear the second optical filtering arranged and sense photodiode, the light source detection channel and light are provided in the sense channel of source
Transmission channel is mutually perpendicular to.
As being further improved for said program, standard substrate fixed seat, the standard are fixed with by the pipetting device
Standard substrate board is provided in substrate fixed seat, the standard substrate fixed seat is located at sustained height with board rack.
As being further improved for said program, heating unit and temperature controller are provided on the bottom plate, the board is put
It puts and board heating plate is equipped in frame, test solution heating plate is provided in the suction nozzle seat.
Beneficial effects of the present invention:
A kind of magnetic enzyme of the present invention exempts from quantitative fluorescence analysis instrument, and board is placed on board rack, and imbibition piston starts will
Magnetic bead test solution in board picks up, and board rack moves to liquid relief station, and imbibition piston puts the magnetic bead test solution picked up to plate
In the test solution slot of card, then board rack is moved to by testing agency backward so that test solution slot is located at below testing agency, profit
Detection is automatically performed with testing agency, entire reaction process is more intelligent, reduces artificial participation, improves the precision of detection
And efficiency.
Description of the drawings
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described.Obviously, described attached drawing is the part of the embodiment of the present invention rather than all implements
Example, without creative efforts, other that can also be obtained according to these attached drawings are set those skilled in the art
Meter scheme and attached drawing:
Fig. 1 is present pre-ferred embodiments structure diagram;
Fig. 2 is present pre-ferred embodiments structure diagram;
Fig. 3 is present pre-ferred embodiments pipetting device and the structure diagram of testing agency;
Fig. 4 is the structure diagram of present pre-ferred embodiments pipetting device;
Fig. 5 is the structure diagram of present pre-ferred embodiments pipetting device;
Fig. 6 is the structure diagram of present pre-ferred embodiments pipetting device;
Fig. 7 is the front view of present pre-ferred embodiments testing agency;
Fig. 8 is the structure diagram of present pre-ferred embodiments testing agency;
Fig. 9 is the sectional view in AA directions in Fig. 7.
Specific embodiment
The technique effect of the design of the present invention, concrete structure and generation is carried out below with reference to embodiment and attached drawing clear
Chu is fully described by, to be completely understood by the purpose of the present invention, feature and effect.Obviously, described embodiment is this hair
Bright part of the embodiment rather than whole embodiments, based on the embodiment of the present invention, those skilled in the art is not paying
The other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.
Referring to figs. 1 to Fig. 9, a kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument, including bottom plate 1, is placed on the bottom plate 1 several
The identical pipetting device 2 of structure, the pipetting device 2 include bearing support 21, are provided on the bearing support 21 and move forward and backward group
Part 8, described move forward and backward are provided with board rack 22, up and down motion component, institute are provided on the bearing support 21 on component 8
Setting mounting blocks 23 on up and down motion component 7 are stated, imbibition piston 24 is fixed on the mounting blocks 23, the imbibition piston 24
Lower end is provided with suction nozzle seat 25, and the suction nozzle 26 being connect with imbibition piston 24, the pipetting device 2 are provided in the suction nozzle seat 25
Rear end be provided with detection drive component 9, it is described detection drive component 9 on be provided with testing agency 3.
With reference to Fig. 3 to Fig. 6, the up and down motion component 7 includes vertical slide rail 71, is provided in the vertical slide rail 71 vertical
Straight sliding block, the mounting blocks 23 are fixed on vertical slipper, and the mounting blocks 23 are connected with the first driving motor 72.It is described front and rear
Moving parts 8 include horizontal slide rail 81, and the horizontal slide rail 81 is equipped with horizontal slider 82, and the board rack 22 is fixed on
On the horizontal slider 82, the board rack 22 is connected with the second driving motor 83.
Mounting blocks 23 move up and down along vertical slide rail 71, and the mounting blocks 23 move downward during imbibition so that suction nozzle seat 25
Interior suction nozzle 26 is extended downwardly into the test solution slot of board, and then the piston block of imbibition piston 24 moves upwards, and suction nozzle 26 is taken out upwards
Test solution is taken to complete imbibition.After the completion of imbibition, mounting blocks 23 move upwards, and board is facilitated to move backward so that the empty slot in board
In 26 lower section of suction nozzle, then the piston block of imbibition piston 24 moves downward so that and the test solution in suction nozzle 26 is expelled in empty slot,
Entire imbibition discharge opeing process is automatically finished, and processing efficiency greatly improves.
Electric rotating machine 27 is provided on the bearing support 21, the main shaft of the electric rotating machine 27 is hinged with by transmission mechanism
Connecting rod 28, the end of the connecting rod 28 are provided with magnet 29, and the electric rotating machine 27 drives connecting rod 28 to be rotated around hinge joint so that
Magnet 29 is separate or close to board rack 22.The transmission mechanism is gear set, and gear set includes being set in electric rotating machine 27
On first gear 61, hinged second gear 62 and the connection first gear 61 and second gear 62 with connecting rod 28
Transmission gear 63.
During imbibition, connecting rod 28 is with moving magnet 29 close to board rack 22 so that and the magnetic bead in board is moved and is assembled,
So that the magnetic bead movement stirring being deposited in board so that magnetic bead reagent is more uniform, and suction nozzle 26 can be facilitated to draw magnetic
Pearl test solution.When the test solution of absorption is expelled in the detect tank of board, connecting rod 28, far from board rack 22, makes with moving magnet 29
It obtains magnetic bead to reset, smart structural design, substantially increases the efficiency of imbibition and liquid relief.
With reference to Fig. 7 to Fig. 9, the testing agency 3 moves the mounting base 31 being arranged on detection drive component, the installation
Detection hole 32 is provided on seat 31,32 lower section of detection hole is connected with light transmission channel 33, the light transmission channel 33
The xenon lamp 34 of transmitting light is inside provided with, the photomultiplier 35 for receiving light, the light are provided with by the detection hole 32
The front end of electric multiplier tube 35 is provided with detection lens subassembly, and the detection lens subassembly includes lens mount 310, sets from front to back
Put convex lens 311, the first optical filtering 312 and acquisition photodiode 313 in lens mount 310.
The mounting base 31 is fixedly arranged above detection scanner 36, and the detection scanner 36 is located at the upper of detection hole 32
Side, detection scanner 36 carry out reading code scanning to the bar code on board, test solution information can be differentiated, with photosignal one
It plays storage and carries out subsequent data processing.
The detection drive component 9 includes detection sliding rail 91, detects and detection sliding block 92 is provided on sliding rail 91, detect sliding block
92 are connected with linear drive apparatus, and the mounting base 31 is fixed on detection sliding block 92, and linear drive apparatus can select straight line
Motor, screw straight-line motion mechanism different from feed screw nut, driving pulley etc..
Xenon lamp 34 emit xenon light enter detection board detect tank in, xenon light is emitted from detect tank, convex lens 311 into
Ambient light is filtered by the collection of row light, the first optical filtering 312, prevents veiling glare from influencing as a result, improving accuracy of detection, acquisition
The optical signal of light is acquired by photodiode 313.Then photomultiplier 35 converts optical signals into electric signal transmission,
Fluorescence relative intensity is provided according to the power of electric signal, then analyzes the concentration of PCT, is finally calculated as a result, realizing to examination
Liquid automatically analyzes detection, substantially increases the rate and precision of detection.
The lower section of the detection hole 32 is also associated with light source detection channel 37, before being provided in the light source detection channel 37
The second optical filtering 38 and sense photodiode 39 arranged afterwards, the light source detection channel 37 are mutual with light transmission channel 33
Vertically, light source detection channel 37 is the intensity for detecting transmitting light, ensures that the intensity of transmitting light is consistent, light is protected when detecting
Hold qualitative, raising accuracy of detection.
Standard substrate fixed seat 4 is fixed with by the pipetting device 2, standard is provided in the standard substrate fixed seat 4
Substrate board 5, the standard substrate fixed seat 4 are located at sustained height, before detection starts, testing agency with board rack 22
3 move to 4 lower section of standard substrate fixed seat, play the role of the comparison of initial position, ensure xenon lamp 34 and photomultiplier 35
It can emit and receive light, play the role of positioning and harmonize.
Heating unit 10 and temperature controller 11 are provided on the bottom plate 1, heating unit is internally heated whole equipment,
Board heating plate is equipped in the board rack 22, is provided with test solution heating plate 210 in the suction nozzle seat 25 so that test solution exists
A stationary temperature is constantly in process, will not be cooled down, using the temperature inside 11 detection device of temperature controller, is ensured
The temperature of reaction is in ideal range, will not be excessively high or too low so that the reaction result of test solution is relatively reliable.
It is that presently preferred embodiments of the present invention is illustrated above, but the invention is not limited to the implementation
Example, those skilled in the art can also make various equivalent modifications under the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent modifications or replacement are all contained in the application claim limited range.
Claims (10)
1. a kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument, including bottom plate (1), it is characterised in that:It is placed on the bottom plate (1) several
The identical pipetting device of structure (2), the pipetting device (2) include bearing support (21), before being provided on the bearing support (21)
Moving parts afterwards, described move forward and backward are provided with board rack (22), are provided on the bearing support (21) on component (7)
Lower moving parts (8), setting mounting blocks (23) on the up and down motion component (8) are fixed with imbibition on the mounting blocks (23)
Piston (24), the lower end of the imbibition piston (24) are provided with suction nozzle seat (25), are provided in the suction nozzle seat (25) and imbibition
The suction nozzle (26) of piston (24) connection, the rear end of the pipetting device (2) are provided with detection drive component (9), and the detection is driven
Testing agency (3) is provided on dynamic component (9).
2. magnetic enzyme according to claim 1 exempts from quantitative fluorescence analysis instrument, it is characterised in that:It is set on the bearing support (21)
There is electric rotating machine (27), the main shaft of the electric rotating machine (27) is hinged with connecting rod (28), the connecting rod by transmission mechanism (6)
(28) end is provided with magnet (29), and the electric rotating machine (27) drives connecting rod (28) to be rotated around hinge joint so that magnet
(29) far from or close to board rack (22).
3. magnetic enzyme according to claim 2 exempts from quantitative fluorescence analysis instrument, it is characterised in that:The transmission mechanism (6) is tooth
Wheel group, gear set include the first gear (61) being set on electric rotating machine (27), the hinged second gear with connecting rod (28)
(62) and the connection first gear (61) and the transmission gear (63) of second gear (62).
4. magnetic enzyme according to claim 1 exempts from quantitative fluorescence analysis instrument, it is characterised in that:The up and down motion component (7)
Including vertical slide rail (71), vertical slipper is provided on the vertical slide rail (71), the mounting blocks (23) are fixed on vertical cunning
On block, the mounting blocks (23) are connected with the first driving motor (72).
5. magnetic enzyme according to claim 1 exempts from quantitative fluorescence analysis instrument, it is characterised in that:It is described to move forward and backward component (8)
Including horizontal slide rail (81), the horizontal slide rail (81) is equipped with horizontal slider (82), and the board rack (22) is fixed on
On the horizontal slider (82), the board rack (22) is connected with the second driving motor (83).
6. magnetic enzyme according to claim 1 exempts from quantitative fluorescence analysis instrument, it is characterised in that:The testing agency (3) includes
It is mobile to be arranged on the mounting base (31) detected on drive component (9), detection hole (32) is provided on the mounting base (31), it is described
Light transmission channel (33) is connected with below detection hole (32), the xenon of transmitting light is provided in the light transmission channel (33)
Light lamp (34), the detection hole (32) is other to be provided with the photomultiplier (35) for receiving light, the photomultiplier (35)
Front end is provided with detection lens subassembly, and the mounting base (31) is fixedly arranged above detection scanner (36), the detection scanning
Instrument (36) is positioned at the top of detection hole (32).
7. magnetic enzyme according to claim 6 exempts from quantitative fluorescence analysis instrument, it is characterised in that:The detection lens subassembly includes
Lens mount (310), the convex lens (311) being arranged on from front to back in lens mount (310), the first optical filtering (312) and is adopted
Collect photodiode (313).
8. magnetic enzyme according to claim 6 exempts from quantitative fluorescence analysis instrument, it is characterised in that:The lower section of the detection hole (32)
Light source detection channel (37) is also associated with, front and rear the second optical filtering (38) arranged is provided in the light source detection channel (37)
With sense photodiode (39), the light source detection channel (37) is mutually perpendicular to light transmission channel (33).
9. magnetic enzyme according to claim 1 exempts from quantitative fluorescence analysis instrument, it is characterised in that:It is solid by the pipetting device (2)
Surely there is standard substrate fixed seat (4), standard substrate board (5), the standard back are provided in the standard substrate fixed seat (4)
Object fixed seat (4) is with board rack (22) positioned at sustained height.
10. magnetic enzyme according to claim 1 exempts from quantitative fluorescence analysis instrument, it is characterised in that:It is provided on the bottom plate (1)
Heating unit (10) and temperature controller (11), the board rack (22) is interior to be equipped with board heating plate, and the suction nozzle seat (25) is interior
It is provided with test solution heating plate (210).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201711256025.8A CN108169501A (en) | 2017-12-04 | 2017-12-04 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201711256025.8A CN108169501A (en) | 2017-12-04 | 2017-12-04 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
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CN108169501A true CN108169501A (en) | 2018-06-15 |
Family
ID=62524237
Family Applications (1)
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CN201711256025.8A Pending CN108169501A (en) | 2017-12-04 | 2017-12-04 | A kind of magnetic enzyme exempts from quantitative fluorescence analysis instrument |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020134630A1 (en) * | 2018-12-29 | 2020-07-02 | 苏州汶颢微流控技术股份有限公司 | Elisa analyzer |
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JPH06313767A (en) * | 1993-04-28 | 1994-11-08 | Medeika Tec Kk | Inspection apparatus for blood and the like using magnetic bead |
US20100126286A1 (en) * | 2007-04-06 | 2010-05-27 | Brian Austin Self | Open platform automated sample processing system |
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WO2020134630A1 (en) * | 2018-12-29 | 2020-07-02 | 苏州汶颢微流控技术股份有限公司 | Elisa analyzer |
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