CN210775522U - Colloidal gold fluorescence integrated immunoassay instrument - Google Patents
Colloidal gold fluorescence integrated immunoassay instrument Download PDFInfo
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- CN210775522U CN210775522U CN201921698470.4U CN201921698470U CN210775522U CN 210775522 U CN210775522 U CN 210775522U CN 201921698470 U CN201921698470 U CN 201921698470U CN 210775522 U CN210775522 U CN 210775522U
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 50
- 238000003018 immunoassay Methods 0.000 title claims description 16
- 238000012360 testing method Methods 0.000 claims abstract description 86
- 238000001514 detection method Methods 0.000 claims abstract description 51
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 19
- 230000036039 immunity Effects 0.000 claims abstract description 9
- 230000001360 synchronised effect Effects 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 100
- 230000005540 biological transmission Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 230000033001 locomotion Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 206010070834 Sensitisation Diseases 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
- 230000008313 sensitization Effects 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
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Abstract
The utility model provides a colloidal gold fluorescence integrated immunity analyzer, which comprises a supporting rotating part, a sample feeding component, a colloidal gold detection module, a fluorescence detection module and a sample discharging component; the top of the bearing seat of the supporting and rotating part is provided with a driving device, the lower part of the bearing seat is sleeved with a turntable, and the turntable is provided with at least one test card frame of a sample feeding assembly; the camera device of the colloidal gold detection module and the fluorescence module of the fluorescence detection module are respectively arranged above the sample area of the test card rack, and detection data are respectively processed by the first data acquisition board and the second data acquisition board; the push-out plate of the sample push-out assembly can move on the second electric linear sliding table and push out the sample card from the test card frame, and the second electric linear sliding table is arranged at the top of the bearing seat.
Description
Technical Field
The utility model belongs to the technical field of the medical treatment detects, concretely relates to integrative immunoassay appearance of colloidal gold fluorescence.
Background
With the development of medical detection technology, researchers have developed various methods and instruments for qualitative and quantitative detection of different human samples. The common detection methods of the human body sample comprise colloidal gold immunoassay detection and fluorescence immunoassay detection, the test range of the fluorescence immunoassay detector is wider than that of the colloidal gold immunoassay detector, and a detector needs to compare the two test methods for the individual human body sample, so that the test and data comparison need to be carried out on the colloidal gold detector and the fluorescence detector, the workload and labor intensity of the detector are increased virtually, and the installation and maintenance of the detector are increased. Technical personnel in the field have developed colloidal gold and fluorescence immunoassay and detect all-in-one, can realize carrying out colloidal gold and fluorescence immunoassay and detect in an instrument, and the data contrast is simple clear, has enlarged human sample's test range, has promoted detection effect and speed, greatly reduced detectioner's working strength, simplified instrument structure, the installation of being convenient for is maintained.
Patent CN201710046763.3 discloses a colloidal gold fluorescence quantitative analysis all-in-one machine and a control method thereof, the all-in-one machine can be used as a colloidal gold card reader and an immunofluorescence analyzer through the action of a microprocessor, a lighting unit, a shooting unit, an image processing unit, a laser and a first photoelectric sensor which are connected with the microprocessor, so that the all-in-one machine can be used as the colloidal gold card reader, and the cost is saved. The all-in-one machine reduces the experiment steps and improves the experiment efficiency.
Patent CN201210449370.4 provides a high biological safety instant detection instrument based on image or sensitization scanning, including image or sensitization scanning device, reagent place the platform and data processing device, the image scanning district of image or sensitization scanning device is downward, and reagent place the platform is located image or sensitization scanning device's image scanning district under, and reagent place the platform is portable, and operating personnel need not to contact the detect reagent who has added biological sample during the detection, improves biological safety.
The existing colloidal gold and fluorescence integrated detection instrument is immature in development and single in form, cannot be flexibly applied when a large quantity of samples are detected, and is inconvenient in maintenance and replacement of parts due to low integration degree of the existing detection instrument.
SUMMERY OF THE UTILITY MODEL
The utility model provides a colloidal gold fluorescence integrated immunity analyzer, which comprises a supporting rotating part, a sample feeding component, a colloidal gold detection module, a fluorescence detection module and a sample discharging component; the supporting and rotating part comprises a bearing seat, a driving device and a turntable, the sample injection assembly comprises at least one test card frame, the colloidal gold detection module comprises a camera device and a first data acquisition board, the fluorescence detection module comprises a fluorescence module and a second data acquisition board, and the sample outlet assembly comprises a push-out board and a second electric linear sliding table.
The top of the bearing seat of the supporting and rotating part is provided with a driving device, the lower part of the bearing seat is sleeved with a turntable, and the turntable is provided with at least one test card frame of a sample feeding assembly; the camera device of the colloidal gold detection module and the fluorescence module of the fluorescence detection module are respectively arranged above the sample area of the test card rack, and the first data acquisition board and the second data acquisition board are used for processing detection data; the electronic sharp slip table of second of going out the appearance subassembly is used for supporting the removal of ejector plate, the ejector plate is used for pushing out the sample card the test card frame, the electronic sharp slip table of second sets up the bearing frame top.
The supporting and rotating part is sequentially provided with a bottom plate, a base, a turntable and a driving connecting device from bottom to top; the driving connecting device comprises a driving device, a driving belt wheel, a synchronous belt, a driven belt wheel and a bearing seat.
The bottom plate is horizontally arranged to support all components of the analyzer, so that the analyzer is stably arranged and operated. The base and the turntable are sequentially connected with the bearing seat, specifically, the base is sleeved at the bottom of the bearing seat to support the turntable and the driving connecting device, the turntable is arranged above the base and sleeved on the periphery of the outside of the bearing seat, and the turntable can be driven by the bearing seat to rotate around the center of a circle.
Preferably, the base and the turntable are circular; preferably, the bearing seat is cylindrical in shape.
The top of bearing frame fixed connection the driven pulleys, one side of driven pulleys is equipped with driving pulley, by between driven pulleys and the driving pulley hold-in range is connected, driving pulley upper portion is connected drive arrangement.
Preferably, the driving device is fixed to the top of the bearing seat through a first mounting plate, that is, the first mounting plate is arranged above the bearing seat, and the driving device is fixed to the upper surface of the first mounting plate. Preferably, the outer side surfaces of the driven pulley and the driving pulley are provided with toothed concave-convex grooves, namely, the driven pulley and the driving pulley are both gears. Preferably, one side of the synchronous belt facing the driven pulley and one side of the driving pulley are provided with toothed concave-convex grooves, namely the synchronous belt is a toothed synchronous belt. The toothed synchronous belt, the driven belt wheel and the driving belt wheel are meshed with each other and can drive each other.
The driving belt wheel, the driven belt wheel and the bearing seat all take respective centers as shafts to do circular motion.
Preferably, the driving device is a stepping motor.
When the device is used, the driving device is powered on to drive the driving belt wheel to rotate, the driving belt wheel drives the driven belt wheel to rotate through the tooth-shaped synchronous belt, the driven belt wheel drives the bearing block to rotate, and the bearing block can drive the rotary table to rotate; in the rotating process, the driving device, the first mounting plate and the base are fixed, and the driving belt wheel, the driven belt wheel and the bearing seat do circular motion by taking respective centers as shafts.
The sample feeding assembly comprises at least one test card frame, a photoelectric sensor and a first Hall sensor. The test card rack is detachably arranged on the upper surface of the turntable and used for placing a sample card; preferably, the area of the test card holder for placing the sample card is a sample area; preferably, the plurality of test card racks are uniformly arranged along the circumference of the turntable; preferably, two opposite inner side surfaces of the test card rack are provided with sliding grooves for clamping the sample card and serving as a sliding way for the sample card to enter and move out.
The photoelectric sensor is arranged above the sample area, and when the analyzer is provided with a plurality of test card frames, the photoelectric sensor is used for detecting whether the sample card is pushed to the root sample area of the test card frame or not, so that the sample card is prevented from being not inserted or not inserted in place.
Preferably, the photoelectric sensor is fixed at one end of the second mounting plate, and the other end of the second mounting plate is fixedly connected with the first mounting plate above the bearing seat. When the test card rack is used, the photoelectric sensor and the second mounting plate are fixed and do not rotate, and the photoelectric sensor detects whether a sample card below the photoelectric sensor pushes a root sample area of the test card rack or not.
The first Hall sensor is arranged outside the rotary disc and below the rotary disc and used for detecting the position of the test card frame. Preferably, the first hall sensor is fixed at one end of the support in the horizontal direction, the support is a T-shaped support, the bottom end of the support is fixed on the bottom plate, and the top end of the support is used for supporting the sample outlet assembly.
Preferably, a sample card outlet is formed in the position, corresponding to the test card frame, of the support, and after the test is completed, the sample card outlet provides an outlet for the sample card to move out of the test card frame.
The rotation condition of card frame and carousel guarantees that the carousel rotates a week and all test card frames can both pass through hall sensor. When the sample feeding assembly works, an operator loads a sample card into the test card frame, the test card frame is driven by the rotary disc to rotate to the position above the Hall sensor, and the Hall sensor records a rotation zero position, namely, the initial rotation position of the test card frame is detected.
The colloidal gold detection module comprises a camera device, a third mounting plate and a first data acquisition plate, and the camera device is arranged above the sample area of the test card frame and is used for acquiring images of samples; one end of the third mounting plate is connected with the camera device, and the other end of the third mounting plate is connected with the first mounting plate, namely, the camera device is fixed on the first mounting plate through the third mounting plate; the first data acquisition board is arranged on the third mounting board and is connected with the camera device through a circuit.
Because the third mounting panel of colloidal gold detection module and the second mounting panel of advancing kind subassembly all set up on first mounting panel, and is preferred, the third mounting panel setting is in the opposite of second mounting panel, promptly colloidal gold detection module with the photoelectric sensor position is separately.
When the test card rack is used, when the test card rack moves to the position below the camera device, the camera device collects sample image information and detects the sample image information, and detection data are transmitted to the first data collecting board, so that colloidal gold detection of one test card rack can be completed; and repeating the detection steps when the next test card frame moves to the position below the camera device along with the turntable.
The fluorescence detection module comprises a fluorescence module, a second Hall sensor, a second data acquisition board and a first electric linear sliding table, wherein the fluorescence module, the second Hall sensor and the second data acquisition board are all installed on the first electric linear sliding table, and one end of the first electric linear sliding table is fixed on the first installation board. Specifically, the fluorescence module is arranged at the lower part of the first electric linear sliding table and is positioned above the sample area of the test card frame, and the fluorescence module can move along the first electric linear sliding table; the second Hall sensor is arranged at the lower part of the first electric linear sliding table and used for controlling the movement stroke of the fluorescent module.
Preferably, the second data acquisition board is arranged at the top of the first electric linear sliding table.
Preferably, one end of a motor of the first electric linear sliding table is arranged on the first mounting plate, and the other end of the motor is arranged above the test card frame in a suspended manner; the lower part of the first electric linear sliding table is provided with a first guide rail and a sliding block, the direction of the first guide rail points to the outer edge of the rotary table from the circle center of the rotary table, namely the direction of the first guide rail is the radius direction of the rotary table; the slider is free to move along the first guide rail.
Preferably, the top of first electronic sharp slip table is equipped with a transmission fixed plate, the position of transmission fixed plate is unmovable, is used for connecting or fixing the part that does not need to remove in the fluorescence detection module the utility model discloses a specific embodiment, the top of transmission fixed plate is fixed with the second data acquisition board.
Preferably, the fluorescent module is connected to the slider of the first electric linear sliding table through a fourth mounting plate and is connected to the motor of the first electric linear sliding table through a circuit, and the fluorescent module and the fourth mounting plate can move along with the slider in the radius direction of the rotary table under the driving of the motor. When the fluorescence detection device is used, the fluorescence module moves from one end of the sample area of the test card frame to the other end of the sample area to finish the detection of a sample.
Preferably, the second hall sensor is fixed at the lower part of the first electric linear sliding table through a fifth mounting plate; more preferably, one end of the fifth mounting plate is fixed at the top of the first electric linear sliding table, and the other end of the fifth mounting plate is connected with the second hall sensor and is located at the lower part of the first electric linear sliding table; more preferably, one end of the fifth mounting plate is fixed on the transmission fixing plate.
The detection range of the second hall sensor is not smaller than the moving range of the fluorescent module, and the position of the second hall sensor can be arranged on the side surface of the sliding block, the side surface of the fourth mounting plate or the side surface of the fluorescent module.
When the device is used, the sliding block drives the fluorescence module to move, the fluorescence module moves from one end of the sample area to the other end of the sample area, the sample card is detected in the moving process, the second Hall sensor does not move and controls the moving stroke of the fluorescence module, and the detection data of the fluorescence module is transmitted to the second data acquisition board.
The sample outlet assembly comprises a third Hall sensor, a second electric linear sliding table and a push-out plate, one end of a motor of the second electric linear sliding table is fixed on the first mounting plate, and the other end of the motor is supported at the top end of the support; the top end of the push-out plate is arranged on the second electric linear sliding table and can freely move along the second electric linear sliding table, and the bottom end of the push-out plate is suspended at the position of the test card frame corresponding to the sample card; and the third Hall sensor is arranged on the second electric linear sliding table and used for controlling the moving stroke of the push-out plate.
Preferably, one end of a motor of the second electric linear sliding table is fixed on the first mounting plate through a sixth mounting plate.
Preferably, the second electric linear sliding table comprises a second guide rail, the top end of the ejector plate is arranged on the second guide rail and is connected with a motor of the second electric linear sliding table through a circuit, and thus the ejector plate can move along the second guide rail in the radial direction of the rotary table. When the pushing plate moves outwards along the turntable, the bottom end of the pushing plate pushes against the sample card, the sample card is pushed out of the test card frame, and finally the analyzer is moved out of the sample card outlet of the support.
Preferably, the third hall sensor is arranged at the lower part of one side, close to the support, of the second electric linear sliding table and used for controlling the moving stroke of the push-out plate.
The colloidal gold detection module, the fluorescence detection module and the card outlet assembly can be simultaneously installed on the first installation plate, and can also be respectively installed according to the detection sequence or requirements, so that the application is flexible, and the maintenance and replacement of parts are convenient.
Preferably, the analyzer has a data transmission function capable of transmitting the detection information of the first data collecting board and the second data collecting board to an external device so as to analyze the detection result.
Preferably, the first data acquisition board, the second data acquisition board, the first hall sensor, the second hall sensor, the third hall sensor, the photoelectric sensor, the camera device and the fluorescence module are provided with an internal power supply or an external power supply.
The utility model also provides a use method of integrative immunoassay appearance of colloidal gold fluorescence, the method includes following step:
(1) switching on a power supply of the analyzer, loading a sample card into the test card frame, turning on the driving device, and driving the test card frame to rotate by the turntable;
(2) the Hall sensor detects and records a rotation zero position, namely, a test card frame is recorded as the zero position, and the first Hall sensor controls the rotation of the turntable;
(3) the camera device of the colloidal gold detection module sequentially aligns the sample area detection data of each test card rack and transmits the data to the first data acquisition board;
(4) when the test card frame moves to the position below the fluorescent module, the fluorescent module moves on a first guide rail of the first electric linear sliding table and moves from one end of a sample area of the test card frame to the other end of the sample area, the fluorescent module detects sample data and transmits the data to the second data acquisition board, and the second Hall sensor controls the movement stroke of the fluorescent module;
(5) when the test card frame which is detected to be completed moves to the sample outlet assembly, the pushing plate moves on the second guide rail of the second electric linear sliding table to push the sample card out of the test card frame, and the third Hall sensor controls the moving stroke of the pushing plate.
Drawings
FIG. 1 is a structural diagram of the supporting rotary part and the sample injection assembly of the present invention
FIG. 2 is the structure diagram of the colloidal gold detection module of the present invention
FIG. 3 is a structural diagram of the fluorescence detection module of the present invention
FIG. 4 is a structural diagram of the sample outlet assembly of the present invention
FIG. 5 is a schematic view of the colloidal gold fluorescence integrated immunoassay analyzer of the present invention
In the attached drawing, 1-stepping motor, 2-driving pulley, 3-tooth-shaped synchronous belt, 4-first mounting plate, 5-second mounting plate, 6-photoelectric sensor, 7-bearing seat, 8-test card frame, 9-rotary table, 10-driven pulley, 11-base, 12-bottom plate, 13-first Hall sensor, 14-bracket, 15-first data acquisition plate, 16-third mounting plate, 17-camera device, 18-fluorescent module, 19-fourth mounting plate, 20-transmission fixing plate, 21-fifth mounting plate, 22-second data acquisition plate, 23-first electric linear sliding table, 24-second Hall sensor, 25-second electric linear sliding table, 26-sixth mounting plate, 27-push plate, 28-third Hall sensor, 29-sample card, 30-sample card outlet.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Example 1 supporting a rotating part and a sample introduction Assembly
The structure of the supporting and rotating part and the sample feeding assembly of this embodiment is shown in fig. 1, and the supporting and rotating part comprises a bottom plate 12, a base 11, a turntable 9 and a driving connection device from bottom to top; the driving connection device comprises a stepping motor 1, a driving belt pulley 2, a tooth-shaped synchronous belt 3, a driven belt pulley 10 and a bearing seat 7.
The base plate 12 is horizontally disposed to support all components of the analyzer and to ensure stable placement and operation of the analyzer. Base 11 cup joints in bearing frame 7 bottom, supports carousel 9 and drive connecting device, and carousel 9 establishes in base 11 top and cup joints around bearing frame 7 outside to can rotate under the drive of bearing frame 7.
The top of bearing frame 7 is fixed connection driven pulley 10, and one side of driven pulley 10 is equipped with driving pulley 2, is connected by profile of tooth hold-in range 3 between driven pulley 10 and the driving pulley 2, and step motor 1 is connected on 2 upper portions of driving pulley. The stepping motor 1 is fixed on the top of the bearing seat 7 through the first mounting plate 4. The outer side surfaces of the driven belt wheel 10 and the driving belt wheel 2 are provided with tooth-shaped concave-convex grooves, the tooth-shaped synchronous belt 3 is provided with tooth-shaped concave-convex grooves on one side facing the driven belt wheel 10 and the driving belt wheel 2, and the tooth-shaped synchronous belt 3 is mutually meshed with the driven belt wheel 10 and the driving belt wheel 2 and can be mutually driven.
When the device is used, the stepping motor 1 is powered on to drive the driving belt pulley 2 to rotate, the driving belt pulley 2 drives the driven belt pulley 10 to rotate through the toothed synchronous belt 3, the driven belt pulley 10 drives the bearing seat 7 to rotate, and then the bearing seat 7 can drive the rotary table 9 to rotate; in the rotating process, the stepping motor 1, the first mounting plate 4 and the base 11 are fixed, and the driving pulley 2, the driven pulley 10 and the bearing seat 7 do circular motion by taking respective centers as axes.
The sample feeding assembly comprises twelve test card frames 8, a photoelectric sensor 6 and a first Hall sensor 13. The test card holder 8 is detachably provided on the upper surface of the turntable 9 for holding the sample card 29. The area of the test card frame 8 for placing the sample card 29 is a sample area, the test card frame 8 is uniformly arranged along the circumference of the turntable 9, and two opposite inner side surfaces of the test card frame 8 are provided with sliding grooves for clamping the sample card 29 and serving as a sliding way for the sample card 29 to enter and move out.
The photoelectric sensor 6 is arranged above the sample area and used for detecting whether the sample card 29 is pushed to the root sample area of the test card holder 8 or not, so that the sample card 29 is prevented from being not inserted or not inserted in place. The photoelectric sensor 6 is fixed at one end of the second mounting plate 5, and the other end of the second mounting plate 5 is fixedly connected with the first mounting plate 4. When in use, the photoelectric sensor 6 and the second mounting plate 5 are fixed and do not rotate.
The first hall sensor 13 is disposed outside the turntable 9 and is used for detecting the rotation of the turntable 9 and the test card holder 8. The first Hall sensor 13 is fixed at one end of the support 14 in the horizontal direction, the support 14 is a T-shaped support, the bottom end of the support 14 is fixed on the bottom plate 12, the top end of the support 14 is used for supporting the sample outlet assembly, a sample card outlet 30 is formed in the position, corresponding to the test card frame 8, of the support 14, and after the test is completed, the sample card 29 is moved out of the test card frame and the analyzer from the sample card outlet 30.
When the sample feeding assembly works, an operator loads the sample card 29 into the test card holder 8, the photoelectric sensor 6 is lighted, the sample card 29 is inserted into the sample area at the root of the test card holder 8, the test card holder 8 rotates for a circle under the driving of the rotary table 9, the first Hall sensor 13 is lighted, and the positions of twelve test card holders 8 are sequentially detected.
Example 2 colloidal gold detection Module
The structure diagram of the colloidal gold detection module of this embodiment is shown in fig. 2, the colloidal gold detection module includes a camera device 17, a third mounting plate 16 and a first data acquisition plate 15, the camera device 17 is arranged above the sample area of the test card holder 8 for acquiring the image of the sample; one end of the third mounting plate 16 is connected with the camera device 17, and the other end is connected with the first mounting plate 4; the first data acquisition board 15 is disposed on the third mounting board 16, and is electrically connected to the camera 17.
When the test card rack 8 is used, when the test card rack 8 moves to the position below the camera device 17, the camera device 17 collects sample image information and detects the sample image information, and detection data are transmitted to the first data collecting board 15, so that colloidal gold detection of one test card rack 8 can be completed; when the next test card holder 8 moves to the lower side of the camera device 17 along with the turntable 9, the above detection steps are repeated.
Example 3 fluorescence detection Module
The structure diagram of the fluorescence detection module of the present embodiment is shown in fig. 3, the fluorescence detection module includes a fluorescence module 18, a second hall sensor 24, a second data acquisition board 22 and a first electric linear sliding table 23, and one end of the first electric linear sliding table 23 is fixed on the first mounting board 4. The fluorescence module 18 is arranged at the lower part of the first electric linear sliding table 23 and is positioned above the sample area of the test card frame 8, and the fluorescence module 18 can move along the first electric linear sliding table 23; the second hall sensor 24 is arranged at the lower part of the first electric linear sliding table 23 and is used for controlling the movement stroke of the fluorescent module 18; the second data acquisition board 22 is arranged on the transmission fixing board 20 at the top of the first electric linear sliding table 23.
One end of a motor of the first electric linear sliding table 23 is arranged on the first mounting plate 4, and the other end of the motor is arranged above the test card frame 8 in a hanging manner; the lower part of first electronic sharp slip table 23 is equipped with first guide rail and slider, and the direction of first guide rail is along the outer edge of centre of a circle directional carousel 9 from carousel 9, and the slider can freely move along first guide rail. The top of the first electric linear sliding table 23 is provided with a transmission fixing plate 20, and the position of the transmission fixing plate is not movable, so that the transmission fixing plate is used for connecting or fixing parts which do not need to move in the fluorescence detection module.
The fluorescent module 18 is connected to the slide block of the first electric linear sliding table 23 through a fourth mounting plate 19, and can move along with the slide block in the radius direction of the rotary table 9 under the driving of the motor. In use, the fluorescence module 18 moves from one end of the sample area of the test card holder 8 to the other end of the sample area to complete the detection of the sample.
One end of the fifth mounting plate 21 is fixed on the transmission fixing plate 20, the other end is connected with the second hall sensor 24 and is located at the lower part of the first electric linear sliding table 23, and the second hall sensor 24 is located on the side surface of the sliding block.
When the device is used, the slide block drives the fluorescence module 18 to move, the fluorescence module 18 moves from one end of the sample area to the other end of the sample area, the sample card is detected in the moving process, the second Hall sensor 24 does not move and controls the moving stroke of the fluorescence module 18, and the detection data of the fluorescence module 18 is transmitted to the second data acquisition board 22.
EXAMPLE 4 sample presentation Assembly
The structure of the sample outlet assembly of the present embodiment is shown in fig. 4, and the sample outlet assembly includes a third hall sensor 28, a second electric linear sliding table 25, and a push-out plate 27. One end of a motor of the second electric linear sliding table 25 is fixed on the first mounting plate 4 through a sixth mounting plate 26, and the other end of the motor is supported at the top end of the bracket 14; the second electric linear sliding table 25 comprises a second guide rail, the top end of the pushing plate 27 is arranged on the second guide rail and can freely move along the second guide rail, and the bottom end of the pushing plate is suspended at the position of the test card frame 8 corresponding to the sample card; the third hall sensor 28 is arranged at the lower part of one side of the second electric linear sliding table 25 close to the bracket and is used for controlling the moving stroke of the pushing plate 27.
The push-out plate 27 is connected to the motor of the second electric linear slide table 25 through an electric circuit, so that the push-out plate 27 can move in the radial direction of the turntable 9 along the second guide rail. As ejector plate 27 moves outwardly of carousel 9, the bottom end of ejector plate 27 abuts sample card 29 and pushes sample card 29 out of test card holder 8, eventually removing the analyzer from sample card outlet 30 of rack 14.
Example 5 method of use
The colloidal gold fluorescence integrated immunoassay analyzer of the present embodiment is wholly illustrated as fig. 5, the card inlet assembly, the colloidal gold detection module, the fluorescence detection module and the card outlet assembly are simultaneously installed on the support rotating portion, the analyzer has a data transmission function, and can transmit the detection information of the first data acquisition board 15 and the second data acquisition board 22 to an external device, so as to analyze the detection result. The first data acquisition board 15, the second data acquisition board 22, the first hall sensor 13, the second hall sensor 24, the third hall sensor 28, the photoelectric sensor 6, the camera device 17, and the fluorescence module 18 have a built-in power supply.
The use method of the colloidal gold fluorescence integrated immunity analyzer comprises the following steps:
(1) switching on a power supply of the analyzer, loading the sample card 29 into the test card holder 8, turning on the stepping motor 1, and driving the test card holder 8 to rotate by the turntable 9;
(2) the photoelectric sensor 6 detects whether the sample card 29 is pushed to the root sample area of the test card holder 8, and the first Hall sensor 13 controls the rotary disc 9 to rotate;
(3) the camera device 17 of the colloidal gold detection module sequentially aligns the sample area detection data of each test card holder 8 and transmits the data to the first data acquisition board 15;
(4) when the test card holder 8 moves below the fluorescence module 18, the fluorescence module 18 moves on a first guide rail of a first electric linear sliding table 23 and moves from one end of a sample area of the test card holder 8 to the other end, the fluorescence module 18 detects sample data and transmits the data to a second data acquisition board 22, and a second hall sensor 24 controls the movement stroke of the fluorescence module 18;
(5) when the detected test card holder 8 moves to the sample discharging assembly, the pushing plate 27 moves on the second guide rail of the second electric linear sliding table 25 to push the sample card 29 out of the test card holder 8, and the third hall sensor 28 controls the moving stroke of the pushing plate 27.
Claims (10)
1. A colloidal gold fluorescence integrated immunity analyzer is characterized in that the analyzer comprises a supporting rotating part, a sample introduction component, a colloidal gold detection module, a fluorescence detection module and a sample outlet component; the sample injection assembly comprises at least one test card frame, the colloidal gold detection module comprises a camera device and a first data acquisition board, the fluorescence detection module comprises a fluorescence module and a second data acquisition board, and the sample outlet assembly comprises a push-out board and a second electric linear sliding table;
the top of the bearing seat of the supporting and rotating part is provided with a driving device, the lower part of the bearing seat is sleeved with a turntable, and the turntable is provided with at least one test card frame of a sample feeding assembly; the camera device of the colloidal gold detection module and the fluorescence module of the fluorescence detection module are respectively arranged above the sample area of the test card rack, and the first data acquisition board and the second data acquisition board are used for processing detection data; the electronic sharp slip table of second of going out the appearance subassembly is used for supporting the removal of ejector plate, the ejector plate is used for pushing out the sample card the test card frame, the electronic sharp slip table of second sets up the bearing frame top.
2. The colloidal gold fluorescence integrated immunity analyzer as claimed in claim 1, wherein the supporting rotation part is provided with a bottom plate, a base, a turntable and a driving connection device from bottom to top, the driving connection device comprises a driving device, a driving pulley, a synchronous belt, a driven pulley and a bearing seat.
3. The colloidal gold fluorescence integrated immunity analyzer as claimed in claim 2, wherein the bottom plate is horizontally disposed, the base and the turntable are sequentially connected to the bearing seat, and the turntable is disposed above the base and sleeved outside the bearing seat.
4. The colloidal gold fluorescence integrated immunity analyzer as claimed in claim 2, wherein the top of the bearing seat is fixedly connected with the driven pulley, one side of the driven pulley is provided with a driving pulley, the driven pulley and the driving pulley are connected by the synchronous belt, the upper part of the driving pulley is connected with the driving device, and the driving device is fixed on the top of the bearing seat through a first mounting plate.
5. The colloidal gold fluorescence integrated immunity analyzer as claimed in claim 1, wherein the sample feeding assembly further comprises a photoelectric sensor and a first hall sensor, the test card holder is detachably disposed on the upper surface of the rotary table, the photoelectric sensor is disposed above the sample area, and the first hall sensor is disposed outside the rotary table and fixed on one end of the support in the horizontal direction.
6. The colloidal gold fluorescence integrated immunity analyzer as claimed in claim 1, wherein the colloidal gold detection module further comprises a third mounting plate, the camera device is disposed above the sample area of the test card holder, one end of the third mounting plate is connected with the camera device, the other end is connected with the first mounting plate, and the first data acquisition plate is disposed on the third mounting plate and is connected with the camera device through a circuit.
7. The colloidal gold fluorescence integrated immunoassay analyzer of claim 1, wherein the fluorescence detection module further comprises a second hall sensor and a first electric linear sliding table; fluorescence module, second hall sensor, second data acquisition board are all installed on first electronic sharp slip table, the one end of first electronic sharp slip table is fixed on first mounting panel.
8. The colloidal gold fluorescence integrated immunoassay analyzer of claim 7, wherein the fluorescence module is installed at the lower part of the first electric linear sliding table and is located above the sample area of the test card holder, the fluorescence module can move along the first electric linear sliding table, the second hall sensor is arranged at the lower part of the first electric linear sliding table, and the second data collecting plate is arranged at the top of the first electric linear sliding table.
9. The colloidal gold fluorescence integrated immunoassay instrument of claim 1, wherein the sample outlet assembly further comprises a third hall sensor.
10. The colloidal gold fluorescence integrated immunoassay analyzer of claim 9, wherein one end of the motor of the second electric linear sliding table is fixed on the first mounting plate, and the other end is supported at the top end of the bracket; the top end of the push-out plate is arranged on the second electric linear sliding table and can freely move along the second electric linear sliding table, and the bottom end of the push-out plate is suspended at the position of the test card frame corresponding to the sample card; and the third Hall sensor is arranged on the second electric linear sliding table.
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| CN201921698470.4U CN210775522U (en) | 2019-10-11 | 2019-10-11 | Colloidal gold fluorescence integrated immunoassay instrument |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110531097A (en) * | 2019-10-11 | 2019-12-03 | 北京中航赛维生物科技有限公司 | A kind of colloidal gold fluorescence one immunity analysis instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110531097A (en) * | 2019-10-11 | 2019-12-03 | 北京中航赛维生物科技有限公司 | A kind of colloidal gold fluorescence one immunity analysis instrument |
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