CN115184592B

CN115184592B - Immunoassay detection equipment and detection method

Info

Publication number: CN115184592B
Application number: CN202210725781.5A
Authority: CN
Inventors: 李瑞庆; 郭永峰; 王陈; 余占江; 程小雷; 陈旭; 句江鹏; 张少康
Original assignee: Pro Med Beijing Technology Co ltd
Current assignee: Pro Med Beijing Technology Co ltd
Priority date: 2022-06-24
Filing date: 2022-06-24
Publication date: 2023-09-29
Anticipated expiration: 2042-06-24
Also published as: CN115184592A

Abstract

The invention provides immunoassay detection equipment and a detection method, belonging to the technical field of medical auxiliary equipment; the device comprises a bottom plate, wherein a sample module, a consumable module, a cartridge clip module, an incubation module, a photoelectric data acquisition module, a sampling module and an emergency detection module are arranged on the bottom plate; the sampling module adopts samples extracted by the sample module and uniformly mixes the samples with the consumable module, the cartridge clip module provides reagent cards, the reagent cards are pushed into a rotary table of the incubation module through a card pushing mechanism of the incubation module, after the uniformly mixed samples are dripped on the reagent cards by the sampling module for incubation, the rotary table is rotated to a card returning card pushing mechanism to push the reagent cards to a photoelectric data acquisition module for scanning, and then the reagent cards are pushed to a waste box; the emergency sample is directly placed on the consumable module emergency frame, and the emergency reagent card is directly plugged into the emergency detection module and pushed into the incubation module turntable by the third card pushing mechanism for incubation. The invention improves the convenience of high automation equipment when a plurality of items need to be detected simultaneously, and has high flexibility in item detection and strong maneuverability in emergency operation.

Description

Immunoassay detection equipment and detection method

Technical Field

The invention relates to the technical field of medical auxiliary equipment, in particular to immunoassay detection equipment and a detection method.

Background

Immunoassay is an analysis technique based on characteristic reactions of antigens and antibodies, and is mainly classified into radioimmunoassay, enzyme immunoassay, chemiluminescent immunoassay, fluorescent immunoassay, etc., according to the difference of labeling technique means.

The existing immunity analyzer is divided into two types roughly, the first type is equipment with low automation degree, the project detection is flexible, the first type is equipment with high automation degree, the first type is equipment with low automation degree, complicated manual steps are needed for auxiliary sample adding detection, manual intervention is easy to cause inaccurate detection results, the second type is equipment with high automation degree, the first type is equipment with high detection efficiency and accuracy, when the first type is faced with more projects to be detected simultaneously, a detection channel inherent to a machine cannot be met, and at the moment, the operation of replacing the inherent channel detection projects through manual operation is very complicated and easy to make mistakes.

In the running process of the existing immunoassay detection equipment, after the detection mode is set, the immunoassay detection equipment carries out detection analysis in a whole course according to a preset sequence, if emergency blood samples needing urgent analysis exist, after the detection and analysis of the immunoassay detection device are completed, the emergency blood sample can be analyzed, mobility is lacked, emergency conditions can not be dealt with, and the conventional immunoassay detection device does not have an emergency detection channel.

Disclosure of Invention

The invention aims to solve the technical problems of providing an immunoassay detection device and a detection method, solving the problem that the automation of an instrument is difficult to realize, simultaneously solving the problem of convenience of simultaneous detection of more items of the existing high-automation device, providing an immunoassay analyzer which can detect multiple samples simultaneously, has high module integration level, flexible item detection and accurate and reliable detection result, and adding an emergency detection channel to improve the flexibility of item detection and the maneuverability of operation when encountering emergency.

In order to solve the technical problems, the invention provides the following technical scheme:

the immunoassay detection equipment comprises a shell, wherein the shell comprises a bottom plate, and a sample module, a consumable module, a cartridge clip module, an incubation module, a photoelectric data acquisition module, a sampling module and an emergency detection module are sequentially integrated and installed in the shell; the consumable module is provided with a TIP head, a TIP discarding nozzle with a gradual change structure and an emergency treatment frame, and emergency treatment blood sampling samples can be placed on the emergency treatment frame; the sampling module can pick up or discard the TIP head, pick up the blood sampling sample and the diluent in the sample module, mix the blood sampling sample and the diluent uniformly, and add the mixed sample to the reagent card in the incubation module for reaction; the cartridge clip module is internally provided with the reagent card, and the reagent card for emergency treatment can be inserted into the emergency treatment detection module; a first card pushing mechanism in the incubation module pushes the reagent card in the cartridge clip module into the incubation module; the sensor senses that the reagent card for emergency treatment is inserted into the emergency treatment detection module, and a third card pushing mechanism in the incubation module pushes the reagent card for emergency treatment into the incubation module; and a second card pushing mechanism in the incubation module pushes the incubated reagent card to a waste card box.

Preferably, a sample module and a consumable module are mounted within the housing; the sample module comprises a supporting plate, a guide rail groove is formed in the supporting plate, a blood sampling frame and a diluent frame are inserted into the guide rail groove, and a notch structure is formed in the insertion position of the guide rail groove; the back of the supporting plate is provided with a back plate, the back plate is provided with a collision bead, the tail ends of the blood sampling frame and the diluent frame are respectively provided with collision bead grooves, and a first sensor is installed on a rear baffle of the blood sampling frame.

Preferably, the consumable module comprises a consumable body, a TIP head supporting plate and a mixing disc are arranged at the top of the consumable body, a TIP head disc is arranged above the TIP head supporting plate, an emergency treatment frame and a TIP discarding device are arranged on the side face of the consumable body, a TIP discarding nozzle on the TIP discarding device adopts a gradual change structure, and the upper part of the emergency treatment frame adopts a notch structure and reducing treatment.

Preferably, a cartridge clip module is arranged above the push-clamping mechanism beside the consumable module, the cartridge clip module comprises a cartridge clip supporting plate, at least eight cartridge clip baffles are arranged on the cartridge clip supporting plate, a corresponding reagent box channel is formed between every two cartridge clip baffles, and at least four vertical reagent boxes are inserted into the corresponding reagent box channels.

Preferably, a small magnet is arranged between the two cartridge clip baffles up and down, and the small magnet and the upper and lower small magnets on the kit are mutually attracted to complete fixation.

Preferably, the kit comprises an upper kit cover and a lower kit cover, wherein a first guide table is arranged in the lower kit cover, a second guide table is arranged in the upper kit cover, and the two guide tables are matched with each other; the first guide table is a protruding rib and is arranged on the side face of the lower cover of the kit, and the second guide table is provided with a protruding rib in the middle and is arranged in the middle of the inner part of the upper cover of the kit.

Preferably, the reagent card is arranged in the reagent box, the front side and the side of the reagent card are respectively provided with a guide groove, the guide groove on the front side is matched with the convex rib in the middle of the second guide table, and the guide groove on the side is matched with the first guide table.

Preferably, a reagent card outlet structure is arranged at one corner of a pair of surfaces of the lower end guide table of the reagent kit, the reagent card outlet structure comprises a torsion spring and a reagent card outlet structure baffle connected with the torsion spring, and the reagent card outlet structure baffle blocks the outlet of the reagent card.

Preferably, chamfers are respectively arranged on the cartridge clip baffle plate and the cartridge clip supporting plate.

Preferably, the incubation module comprises an upper heat-preserving cover, a lower heat-preserving cover, a rotary power module and a second sensor, wherein the second sensor is arranged on the upper heat-preserving cover, the rotary power module is arranged below the heat-preserving cover, and the rotary power module comprises a rotary table and a rotary table motor.

Preferably, a pressing sheet is arranged on the turntable, a connecting main shaft is connected below the turntable, a bearing sleeve is connected below the bottom plate, the connecting main shaft is fixed on a bearing, a connecting main shaft is arranged on an inner ring of the bearing, and an outer ring of the bearing is arranged in the bearing sleeve; the rotary table synchronous large belt wheel and the expansion sleeve are sequentially installed on the connecting main shaft, the motor installation seat is installed on the bottom plate, the rotary table motor is installed on the motor installation seat, the rotary table synchronous small belt wheel is installed on the output shaft of the rotary table motor, and the rotary table synchronous large belt wheel and the rotary table synchronous small belt wheel are provided with the rotary table synchronous belt.

Preferably, four first pushing and clamping mechanisms are arranged on the bottom plate corresponding to the reagent box channel, a first synchronous belt mechanism is arranged at the top of the bottom plate, a first motor for driving the first synchronous belt mechanism is arranged at the bottom of the bottom plate, the first pushing and clamping mechanisms are arranged on a synchronous belt of the first synchronous belt mechanism, a first sliding block is arranged on the synchronous belt, and the first sliding block can move along the synchronous belt.

Preferably, the first card pushing mechanism comprises a first small torsion spring, the first small torsion spring is sleeved on a first stopper screw, the first small push block is installed on the upper end structure of the first card pushing seat through the first stopper screw and the first small torsion spring, the lower end of the first card pushing seat is installed on the first sliding block, the first small toothed plate is installed on the lower end structure of the first card pushing seat, and the first synchronous belt mechanism drives the first sliding block to conduct reciprocating card pushing movement.

Preferably, the side surface of the bottom plate, which is positioned on the first card pushing mechanism, is provided with a second card pushing mechanism, the top of the bottom plate is provided with a second synchronous belt mechanism, the bottom of the bottom plate is provided with a second motor for driving the second synchronous belt mechanism, the synchronous belt of the second synchronous belt mechanism is provided with the second card pushing mechanism, a second sliding block is arranged on the synchronous belt, and the second sliding block can move along with the synchronous belt.

Preferably, the second card pushing mechanism comprises a second small torsion spring, the second small torsion spring is sleeved on a second stopper screw, the second small pushing block passes through the second stopper screw and the second small torsion spring is arranged on the upper end structure of the second card pushing seat, the lower end of the second card pushing seat is arranged on the second sliding block, the second small toothed plate is arranged on the lower end structure of the second card pushing seat, and the second synchronous belt mechanism drives the second sliding block to conduct reciprocating card pushing movement.

Preferably, the third card pushing mechanism is arranged on the bottom plate near the sample module, a third synchronous belt mechanism is arranged at the top of the bottom plate, a third motor for driving the third synchronous belt mechanism is arranged at the bottom of the bottom plate, the third card pushing mechanism is arranged on a synchronous belt of the third synchronous belt mechanism, a third sliding block is arranged on the synchronous belt, and the third sliding block can move along the synchronous belt.

Preferably, the third card pushing mechanism comprises a third plug screw, a third small torsion spring is sleeved on the third plug screw, a small pushing block is mounted on the upper end structure of the third card pushing seat through the third plug screw and the third small torsion spring, a cam block is mounted on the third card pushing seat, the lower end of the third card pushing seat is mounted on the third sliding block, a third small toothed plate is mounted on the lower end structure of the third card pushing seat, and the third synchronous belt drives the third sliding block to conduct reciprocating card pushing movement.

Preferably, the reagent card for emergency treatment is inserted into an emergency treatment detection channel in the emergency treatment detection module beside the consumable module, a top block is arranged on the emergency treatment detection channel, the top block comprises a fourth spring, one end of the fourth spring is installed on a cartridge clip supporting plate, the other end of the fourth spring is connected with a stop block, the bottom end of the stop block is provided with an L-shaped frame, and a pin is installed on the L-shaped frame; the cartridge clip supporting plate is provided with an inductor for inducing the reagent card of the emergency to be inserted.

Preferably, the photoelectric data acquisition module is arranged on the cartridge clip supporting plate and is connected with the card withdrawing channel, and the photoelectric data acquisition module comprises a luminous box and a reagent card sheet.

Preferably, the sampling module is arranged behind the consumable module on the bottom plate; the sampling module comprises a sampling assembly, an X-axis mechanical arm assembly, a Y-axis mechanical arm assembly and a Z-axis mechanical arm assembly, wherein the X-axis mechanical arm assembly, the Y-axis mechanical arm assembly and the Z-axis mechanical arm assembly can drive the sampled sample X, Y, Z to move in the direction.

Preferably, the X-axis mechanical arm assembly comprises a first frame, a fourth motor is installed at the bottom of the first frame, two first synchronous pulleys are installed at the top of the first frame, one of the first synchronous pulleys is driven by the fourth motor to rotate, a fourth synchronous belt is installed on the two first synchronous pulleys, a first synchronous sliding block is installed on the fourth synchronous belt, a first guide rail and a second guide rail are arranged at the top of the first frame, a fourth sliding block is installed on the first guide rail and the second guide rail, and the Y-axis mechanical arm assembly is installed on the fourth sliding block and the first synchronous sliding block.

Preferably, the Y-axis mechanical arm assembly comprises a second frame mounted on the first and fourth synchronous sliders; two second synchronous pulleys are arranged on the front side surface of the second frame, a fifth motor is arranged on the rear side surface of the second frame, one of the second synchronous pulleys is driven by the fifth motor, a fifth synchronous belt is arranged on the two second synchronous pulleys, a third guide rail is arranged on the front side surface of the second frame, a second synchronous slider is arranged on the fifth synchronous belt and the third guide rail, and the Z-axis mechanical arm assembly is arranged on the second synchronous slider.

Preferably, the Z-axis mechanical arm assembly comprises a third rack, and the third rack is mounted on a second synchronous slide block; two third synchronous pulleys are arranged on the front side surface of the third frame, a sixth motor is arranged on the rear side surface of the third frame, one of the third synchronous pulleys is driven by the sixth motor, a sixth synchronous belt is arranged on the two third synchronous pulleys, a fourth guide rail is arranged on the front side surface of the third frame, a third synchronous sliding block is arranged on the sixth synchronous belt and the fourth guide rail, and the sampling assembly is arranged on the third synchronous sliding block.

Preferably, the sampling assembly comprises a connecting shaft, the top of the connecting shaft is mounted on the third synchronous slide block through a connecting piece, the connecting shaft is connected with a connecting block, a small connecting block is fixed on the connecting block, a TIP head sensor is fixed on the small connecting block, the small connecting shaft is connected with the connecting block, a small spring is sleeved on the small connecting shaft, a sleeve is connected with the small connecting shaft, a lower shell cover and a middle shell cover are mounted with an upper shell cover from bottom to top and cover other parts except the sleeve, a needle head and the small connecting shaft, and the needle head is mounted with the small connecting shaft.

Preferably, the TIP discarding nozzle of the gradual change structure comprises a large-caliber part and a small-caliber part, and the large-caliber part and the small-caliber part are in transitional communication.

Preferably, the shell comprises a bottom plate and a shell, a cabin door is arranged on the shell, the cabin door is arranged outside the sample module and the consumable module, a display screen is arranged above the cabin door, and the display screen is electrically connected with a first sensor on a baffle plate at the rear side of the blood collection frame and the photoelectric data collection module.

An immunoassay detection method comprising the immunoassay detection device, characterized in that the detection method comprises collective detection and emergency detection; the collective detection includes:

inserting a blood sampling frame in the sample module into the sample module, detecting the insertion information of the blood sampling frame by a first sensor, transmitting the information to a control system, automatically inputting one-dimensional code information on the blood sampling frame by a code scanner, and transmitting the one-dimensional code information to the control system, wherein the one-dimensional code information is used for judging the detection sample position and patient information of the blood sampling frame;

after entering detection item information, the control system controls the sampling module and the incubation module, the sampling module sequentially absorbs a blood sampling sample and a diluent, then adds the blood sampling sample and the diluent into a mixing disc for mixing, the first card pushing mechanism pushes the reagent card in the kit into the turntable after mixing, and the sampling module adds the mixed sample into the reagent card for incubation;

After incubation is completed, the turntable is turned to a card returning channel, the second card pushing mechanism pushes the reagent card to the photoelectric data acquisition module, the photoelectric data acquisition module acquires information and transmits the information to the control system, and the second card pushing mechanism pushes the reagent card after incubation to the waste card box;

the emergency detection includes:

the blood collection tube is placed in the emergency treatment frame, and the reagent card of the emergency treatment is inserted into the emergency treatment detection channel of the emergency treatment detection module;

the control system controls the sampling module and the incubation module, the sampling module absorbs the emergency blood sampling sample and the diluent and puts the emergency blood sampling sample and the diluent into the mixing disc for mixing, the inductor senses the insertion of the reagent card of the emergency, the third card pushing mechanism pushes the reagent card of the emergency into the rotating disc, and the sampling module adds the mixed sample into the reagent card of the emergency for incubation;

after incubation is completed, the turntable is turned to a card returning channel, the second card pushing mechanism pushes the reagent card to the photoelectric data acquisition module, the photoelectric data acquisition module acquires information and transmits the information to the control system, and the second card pushing mechanism pushes the reagent card of the emergency after incubation to the waste card box.

Compared with the prior art, the invention has at least the following beneficial effects:

according to the scheme, the immunoassay detection equipment and the detection method solve the problem that instrument automation is difficult to realize, and meanwhile solve the problem of convenience when the existing high-automation equipment needs to detect more items simultaneously.

Drawings

The accompanying drawings, which are incorporated herein and form a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the disclosure and to enable a person skilled in the pertinent art to make and use the disclosure.

FIG. 1 is a schematic diagram of an immunoassay detection device according to the present invention;

FIG. 2 is a schematic structural diagram of a sampling mixing module of the immunoassay detection device of the present invention;

FIG. 3 is a schematic diagram of a cartridge clip module of the immunoassay detection device of the present invention;

FIG. 4 is a schematic diagram of the card pushing mechanism and the incubation module of the immunoassay detection apparatus of the present invention;

FIG. 5 is a schematic diagram of a photoelectric data acquisition module of the immunoassay detection device of the present invention;

FIG. 6 is a schematic diagram of a sampling module of the immunoassay detection device of the present invention;

FIG. 7 is a schematic structural diagram of an emergency detection module of the immunoassay detection device of the present invention;

FIG. 8 is a schematic view showing the structures of a blood sampling rack and a diluent rack of the immunoassay detection device of the present invention;

FIG. 9 is a schematic structural view of a support plate of a sample block of the immunoassay detection device of the present invention;

FIG. 10 is a schematic view of the collision bead connection of a sample module of an immunoassay detection device of the present invention;

FIG. 11 is a schematic view of a first sensor of a sample block of an immunoassay detection device of the present invention;

FIG. 12 is a schematic diagram showing the structure of consumable modules of the immunoassay detection instrument of the present invention;

FIG. 13 is a schematic diagram of an emergency device of a consumable module of an immunoassay detection assembly of the present invention;

FIG. 14 is a schematic view showing the structure of a TIP discard nozzle of a consumable module of an immunoassay detection apparatus of the present invention;

FIG. 15 is a schematic view of a clamping piece of a consumable module of an immunoassay detection assembly of the present invention;

FIG. 16 is a schematic diagram of a second embodiment of a clamping plate of a consumable module of an immunoassay detection assembly of the present invention;

FIG. 17 is a schematic view showing the structure of a lower cover of a kit of the immunoassay detection device of the present invention;

FIG. 18 is a detailed schematic of FIG. 17;

FIG. 19 is a schematic view showing the structure of a reagent kit upper cover of the immunoassay detection device of the present invention;

FIG. 20 is a schematic diagram showing the structure of a reagent card of the immunoassay detection device of the present invention;

FIG. 21 is a schematic structural view of a kit of an immunoassay detection device of the present invention;

FIG. 22 is a schematic diagram II of a cartridge clip module of the immunoassay detection device of the present invention;

FIG. 23 is an exploded view of a cartridge clip of the cartridge clip module of the immunoassay detection instrument of the present invention;

FIG. 24 is a schematic view showing the structure of the reagent card outlet of the kit of the immunoassay detection instrument of the present invention;

FIG. 25 is a schematic view showing the structure of a turntable base of the immunoassay detection instrument of the present invention;

FIG. 26 is an exploded view of a first card pushing mechanism of the immunoassay detection instrument of the present invention;

FIG. 27 is an exploded view of a second card pushing mechanism of the immunoassay detection instrument of the present invention;

FIG. 28 is an exploded view of a third card pushing mechanism of the immunoassay detection instrument of the present invention;

FIG. 29 is a schematic view showing a stopper mechanism of a third card pushing mechanism of the immunoassay detection instrument of the present invention;

FIG. 30 is a top view of the immunoassay detection device of the present invention with the cartridge module removed;

FIG. 31 is a schematic diagram showing a third card pushing mechanism and a third card pushing mechanism stopper structure of the immunoassay detection device of the present invention;

FIG. 32 is a second schematic diagram of a third card pushing mechanism and a third card pushing mechanism stopper of the immunoassay detection device of the present invention;

FIG. 33 is a schematic structural view of an X-axis mechanical arm assembly of the immunoassay detection device of the present invention;

FIG. 34 is a schematic view of the Y-axis mechanical arm assembly of the immunoassay detection device of the present invention;

FIG. 35 is a schematic structural view of a Z-axis mechanical arm assembly of the immunoassay detection device of the present invention;

FIG. 36 is a schematic structural view of a sampling assembly of an immunoassay detection device of the present invention;

FIG. 37 is an exploded view of a sampling assembly of the immunoassay detection device of the present invention;

FIG. 38 is an exploded view of a sampling assembly of an immunoassay detection device of the present invention;

FIG. 39 is a schematic diagram of the sample assembly of the immunoassay detection device of the present invention sucking the TIP head;

FIG. 40 is a schematic view showing the appearance of an immunoassay detection device of the present invention;

FIG. 41 is a flow chart of collective detection of the immunoassay detection device of the present invention;

FIG. 42 is an emergency test flow chart of the immunoassay test device of the present invention.

[ reference numerals ]

1. A bottom plate; 2. a sample module; 3. a consumable module; 4. a cartridge clip module; 5. an incubation module; 6. a photoelectric data acquisition module; 7. a sampling module; 8. an emergency detection module;

21. a blood sampling frame; 22. a diluent rack; 23. a support plate; 24. a blood sampling rack guide rail; 25. a diluent rack guide rail; 28. a groove; 29. a ball; 30. a ball collision frame; 211. a first sensor;

31. an emergency treatment frame; 32. TIP head disk; 33. a mixing disc; 34. a TIP head; 35. TIP discard nozzle; 36. a blood collection tube; 370. a clamping piece; 38. a TIP head support plate; 39. a gradual change structure; 40. a bending structure; 301. a notch structure;

41. a clip support plate; 42. small magnet; 43. a kit; 431. a small magnet; 432. a lower cover of the kit; 433. a reagent box upper cover; 434. a slot; 435. a first guide table; 436. a second guide table; 437. a reagent card outlet structure baffle; 44. a clip baffle; 45. a reagent card; 451. a guide groove; 411. chamfering; 461. clip baffle opening structure; 46. a kit channel;

51. An upper heat preservation cover; 52. a lower heat-preserving cover; 53. a rotary power module; 54. a second sensor; 55. a turntable; 56. tabletting; 571. connecting a main shaft; 572. an expansion sleeve; 573. the turntable synchronizes the large belt wheel; 574. the turntable synchronizes the small belt pulley; 575. a turntable motor; 576. a turntable synchronous belt; 577. a motor mounting seat;

11. the first card pushing mechanism; 111. a first motor; 112. a first timing belt mechanism; 113 small push block one; 114. the first push clamping seat; 115. the first push clamping seat is arranged below; 116. a first pinion plate; 117. a first small torsion spring; 118. a first plugging screw; 119. a first slider; 12. the second card pushing mechanism; 121. a second motor; 122. a second timing belt mechanism; 123. a small pushing block II; 124. the second push clamping seat; 125. the second pushing clamping seat is arranged below; 126. a second pinion plate; 127. a second small torsion spring; 128. a second plugging screw; 129. a second slider; 13. a third card pushing mechanism; 131. a third motor; 132. a third timing belt mechanism; 133. a small pushing block III; 134. the third push clamping seat; 135. the third push clamping seat is arranged below; 136. a third pinion rack; 137. a third small torsion spring; 138. thirdly, plugging screws; 139. a third slider; 48. a top block; 49. a cam block; 483. a fourth spring; 183. a stop block; 184. a pin; 185. a wharf is scanned; 484. an inductor;

61. A light emitting box; 62. a reagent clamping piece; 17. an X-axis mechanical arm assembly; 171. a fourth motor; 172. a fourth timing belt; 173. a first guide rail; 174. a second guide rail; 175. a first synchronous pulley; 27. a Y-axis mechanical arm assembly; 271. a fifth motor; 272. a fifth timing belt; 273. a third guide rail; 274. a second synchronous pulley; 37. a Z-axis robotic arm assembly; 371. a sixth motor; 372. a sixth timing belt; 373. a fourth guide rail; 374. a third synchronous pulley; 47. a sampling assembly; 178. a syringe assembly;

471. an upper housing; 472. a middle shell cover; 473. a lower housing; 474. a sleeve; 475. a small spring; 476. a needle; 477. whether a sensor exists in the TIP head; 478. a connecting shaft; 479. a connecting block; 480. connecting small blocks; 481. connecting a small shaft;

100. a housing; 101. a display screen; 102. and (5) a cabin door.

While particular structures and devices are shown in the drawings to enable a clear implementation of embodiments of the invention, this is for illustrative purposes only and is not intended to limit the invention to the particular structures, devices and environments, which may be modified or adapted by those of ordinary skill in the art, as desired, and which remain within the scope of the appended claims.

Detailed Description

The immunoassay detection device and the detection method provided by the invention are described in detail below with reference to the drawings and the specific embodiments. While the invention has been described herein in terms of the preferred and preferred embodiments, the following embodiments are intended to be more illustrative, and may be implemented in many alternative ways as will occur to those of skill in the art; and the accompanying drawings are only for the purpose of describing the embodiments more specifically and are not intended to limit the invention specifically.

It should be noted that references in the specification to "one embodiment," "an example embodiment," "some embodiments," etc., indicate that the embodiment described may include a particular feature, structure, or characteristic, but every embodiment may not necessarily include the particular feature, structure, or characteristic. Further, when a particular feature, structure, or characteristic is described in connection with an embodiment, it is submitted that it is within the knowledge of one skilled in the relevant art to effect such feature, structure, or characteristic in connection with other embodiments whether or not explicitly described.

Generally, the terminology may be understood, at least in part, from the use of context. For example, the term "one or more" as used herein may be used to describe any feature, structure, or characteristic in a singular sense, or may be used to describe a combination of features, structures, or characteristics in a plural sense, depending at least in part on the context. In addition, the term "based on" may be understood as not necessarily intended to convey an exclusive set of factors, but may instead, depending at least in part on the context, allow for other factors that are not necessarily explicitly described.

As used herein, the term "nominal" refers to a range of desired or target values, and higher and/or lower than desired values, for a characteristic or parameter of a component or process operation that is set during a design phase of a production or manufacturing process. The range of values may be due to slight variations in manufacturing processes or tolerances. As used herein, the term "about" indicates a given amount of value that may vary based on the particular technology node associated with the subject semiconductor device. Based on a particular technology node, the term "about" may indicate a given amount of a value that varies, for example, within 5% -15% of the value (e.g., ±5%, ±10% or±15%).

It will be understood that the meanings of "on … …", "over … …" and "over … …" in this disclosure should be interpreted in the broadest sense so that "on … …" means not only "directly on" but also includes meaning "directly on" something with intervening features or layers therebetween, and "over … …" or "over … …" means not only "on" or "over" something, but also may include its meaning "on" or "over" something without intervening features or layers therebetween.

Furthermore, spatially relative terms such as "under …," "under …," "lower," "above …," "upper," and the like may be used herein for ease of description to describe one element or feature's relationship to another element or feature as illustrated in the figures. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. The device may be otherwise oriented and the spatially relative descriptors used herein may similarly be interpreted accordingly.

As shown in fig. 1, an embodiment of the present invention provides an immunoassay detection device, which includes a base plate 1, and a sample module 2, a consumable module 3, a cartridge clip module 4, an incubation module 5, a photoelectric data acquisition module 6, a sampling module 7, and an emergency detection module 8 are installed on the base plate 1.

As shown in fig. 2 and 8 to 11, the sample module 2 includes a support plate 23, the support plate 23 is mounted on the base plate 1, and the blood sampling rack 21 and the dilution rack 22, preferably four rows of the blood sampling racks 21 and one row of the dilution racks 22, are mounted on the support plate 23. The supporting plate 23 is provided with five corresponding guide rail grooves, namely four rows of blood sampling frame guide rails 24 and one row of diluent frame guide rails 25, and the blood sampling frame 21 and the diluent frame 22 are respectively arranged on the blood sampling frame guide rails 24 and the diluent frame guide rails 25 in a drawing way. Specifically, the insertion position of the guide rail groove is provided with a notch structure to form a size gradual change type, the size is from large to small, the guide effect is achieved, the bottoms of the blood sampling frame 21 and the diluent frame 22 are provided with guide blocks correspondingly, the guide blocks can be inserted into the guide rail groove, the shape of the guide blocks is matched with that of the guide rail groove, the insertion position of the guide blocks is provided with a notch structure to form a size gradual change type, the size is from small to large, the blood sampling frame 21 and the diluent frame 22 can be smoothly inserted, and the blood sampling frame 21 is conveniently inserted into the corresponding guide rail groove; the back of backup pad 23 is installed the backplate through the screw, control and install the curb plate through the screw respectively, be equipped with on the backplate and bump the pearl frame 30, the tail end of blood sampling frame 21 and diluent frame 22 is equipped with recess 28, the recess 28 is jogged mutually with the bump pearl 29 on bump the pearl frame 30, realize the location of diluent frame 22 and backplate, the location of blood sampling frame 21 and backplate, bump the fixed design with the guide rail extraction of pearl has promoted the convenience and has promoted compact structure, this design has solved doctor and machine man-machine interaction problem at the sample and has been placed, the experience of instrument use is felt. The first sensor 211 is installed on the rear baffle plate of the position of the blood sampling frame 21, after the first sensor 211 detects that the blood sampling frame 21 is inserted into the guide rail groove, a code scanner installed on the bottom plate 1 automatically inputs one-dimensional code information on the blood sampling frame 21, and therefore the detection sample position and patient information of the blood sampling frame 21 are judged.

The blood sampling rack 21 and the diluent rack 22 are provided with push hands, and the bottoms of the push hands are provided with concave curved surface structures, so that the blood sampling rack is convenient to take; the blood collection frame 21 is provided with a blood collection tube installation unit which is of an open structure, is convenient for installing the blood collection tube 36, and can identify one-dimensional code information of the blood collection tube 36 from the opening of the blood collection tube installation unit.

As shown in fig. 2 and 12-16, the consumable module 3 comprises a consumable body, a TIP head supporting plate 38 and a mixing disc 33 are arranged at the top of the consumable body, an emergency treatment rack 31 and a TIP discarding device are arranged on the side surface of the consumable body, and a TIP head disc 32 is fixed on the TIP head supporting plate 38 through a clamping piece 370; the emergency treatment frame 31 is arranged close to the side wall of the sample module 2, and the upper part of the emergency treatment frame 31 adopts a notch structure 301 and diameter-changing treatment to guide the placement of the blood collection tube 36 to the emergency treatment frame 31; the emergency treatment frame 31 is provided with a TIP discarding device, the TIP discarding device is provided with a TIP discarding nozzle 35, the TIP discarding nozzle 35 adopts a gradual change structure 39, and gradually changes from small to large from the insertion position of the TIP head to the discarding nozzle of the TIP nozzle, thereby being convenient for replacing the TIP head 34; the clamping pieces 370 adopt two-section bending structures 40, one clamping piece 370 is respectively arranged at two sides of the TIP head disk 32, the TIP head supporting plate 38 is simultaneously clamped inwards at two sides to fix the TIP head supporting plate 38 and the TIP head disk 32, and the clamping effect can be achieved when the TIP head disk 32 is replaced; the TIP head disk 32 is provided with a plurality of TIP head 34 placement positions, a mixing disk 33 is arranged beside the TIP head disk 32, the TIP head 34 can be replaced by the sampling module 7 through a TIP discarding nozzle 35, and the sampling module 7 can sequentially add samples in the blood sampling rack 21 and diluent in the diluent rack 22 into the mixing disk 33 for mixing. The TIP discarding nozzle 35 adopts the design of gradual change type opening to improve the efficiency of consumable replacement, the emergency treatment frame 31 adopts the design of opening type structure 301 to improve the convenience of the instrument for emergency treatment, and the bending structure 40 of the clamping piece 370 improves the overall stability of the TIP head disk 32 on the consumable module 3. Below the TIP-discarding nozzle 35 is placed a reject box, and the used TIP head 34 falls directly from the TIP-discarding nozzle 35 into the lower reject box.

As shown in fig. 3 and 17-24, a cartridge clip module 4 is arranged right above the card pushing mechanism of the bottom plate 1, the cartridge clip module 4 comprises a cartridge clip supporting plate 41, a plurality of cartridge clip baffles 44 are arranged on the cartridge clip supporting plate 41, a corresponding reagent box channel 46 is formed between the two cartridge clip baffles 44, and a plurality of reagent boxes 43 which are vertically arranged are inserted into the corresponding reagent box channel 46. The cartridge clip baffle 44 is provided with the cartridge clip baffle opening structure 461, each reagent kit 43 is inserted into the cartridge clip baffle 44 from the cartridge clip baffle opening structure 461, two small magnet stones 42 are arranged between the two cartridge clip baffles 44 up and down, the small magnet stones 42 and the upper and lower small magnets 431 on the reagent kit 43 are mutually attracted and fixed, the design solves the guiding problem of the reagent kit 43, promotes the convenience of replacing the reagent kit 43, solves the accuracy problem of locating the reagent kit 43, and promotes the convenience and stability of replacing the reagent kit 43 of an instrument.

As shown in fig. 17-24, the kit 43 includes an upper kit cover 433 and a lower kit cover 432, a slot 434 for placing a small magnet 431 is provided in the lower kit cover 432, the small magnet 431 is placed in the slot 434, a first guide table 435 is provided in the lower kit cover 432, a second guide table 436 is provided in the upper kit cover 433, and the two guide tables are mutually matched; the first guide table 435 is a protruding rib and is arranged on the side surface of the lower cover 432 of the kit, and the second guide table 436 is provided with a protruding rib in the middle and is arranged in the middle of the inner part of the upper cover 433 of the kit; the reagent card 45 is arranged in the reagent box 43, the front side and the side of the reagent card 45 are respectively provided with a guide groove 451, the guide groove 451 at the front side is matched with a convex rib in the middle of the second 436 of the guide table, the guide groove 451 at the side is matched with the first 435 of the guide table, the function of sliding the reagent card 45 from top to bottom in the reagent box 43 is realized, and side turning can be prevented; the lower cover 432 of the kit is provided with a groove 434, the corresponding position of the upper cover 433 of the kit is provided with a buckle 438, the lower cover 432 of the kit and the upper cover 433 of the kit are buckled together through the groove 434 and the buckle 438, and the complete kit 43 is combined. One corner opposite to the first 435 of the guiding table at the lower end of the reagent box 43 is provided with a reagent card outlet structure, the reagent card outlet structure comprises a torsion spring and a reagent card outlet structure blocking piece 437 connected with the torsion spring, a notch is arranged at the lower end of the reagent card 45, the first card pushing mechanism 11 pushes the reagent card 45 at the notch, the reagent card 45 props against the reagent card outlet structure blocking piece 437, and the torsion spring is stressed to drive the reagent card outlet structure blocking piece 437 to rotate and move away so that the reagent card 45 is pushed into the turntable 55 by the first card pushing mechanism 11.

As shown in fig. 22, the clip baffle 44 and the clip support plate 41 are provided with chamfers 411, respectively, which can guide the reagent kit 43 and facilitate the insertion.

As shown in fig. 4 and 25-29, the incubation module 5 includes an upper heat-insulating cover 51, a lower heat-insulating cover 52, a rotation power module 53, and a second sensor 54, wherein the second sensor 54 is disposed on the upper heat-insulating cover 51, and the rotation power module 53 is disposed below the heat-insulating cover and includes a turntable 55 and a turntable motor 575; the second sensor 54 is operative to detect the presence or absence of the reagent card 45 at the current location, preventing the presence of the reagent card 45 at this point when a homogenized sample is added.

The bottom plate 1 corresponding to the lower part of the incubation module 5 is provided with a card pushing mechanism, namely a first card pushing mechanism 11, a second card pushing mechanism 12 and a third card pushing mechanism 13; the turntable 55 is provided with a pressing sheet 56, a connecting main shaft 571 is connected below the turntable 55, a bearing sleeve is connected below the bottom plate 1, the connecting main shaft 571 is fixed on a bearing, a connecting main shaft 571 is installed on an inner ring of the bearing, and an outer ring of the bearing is installed in the bearing sleeve; a turntable synchronous large belt wheel 573 and an expansion sleeve 572 are sequentially installed on the connection main shaft 571, a motor installation seat 577 is installed on the bottom plate 1, a turntable motor 575 is installed on the motor installation seat 577, a turntable synchronous small belt wheel 574 is installed on an output shaft of the turntable motor 575, and a turntable synchronous belt 576 is installed on the turntable synchronous large belt wheel 573 and the turntable synchronous small belt wheel 574. The turntable motor 575 is driven by the turntable timing belt 576 to rotate the turntable 55.

As shown in fig. 1, 4, 26 and 30, the first card pushing mechanism 11 is provided on the base plate 1 corresponding to the reagent cartridge channels 46, 4 reagent cartridge channels 46 are provided, 4 first card pushing mechanisms 11 are provided corresponding to the reagent cartridge channels, a first synchronous belt mechanism 112 is mounted on the top of the base plate 1, a first motor 111 capable of driving the first synchronous belt mechanism 112 is mounted on the bottom of the base plate 1, the first card pushing mechanism 11 is mounted on the synchronous belt of the first synchronous belt mechanism 112, specifically, a first slider 119 is mounted on the synchronous belt, and the first slider 119 can move along the synchronous belt.

The first card pushing mechanism 11 comprises a first small push block 113, a first card pushing seat upper 114, a first card pushing seat lower 115, a first small toothed plate 116, a first small torsion spring 117 and a first stopper screw 118, wherein the first small torsion spring 117 is sleeved on the first stopper screw 118, the first small push block 113 is arranged on the first card pushing seat upper 114 through the first stopper screw 118 and the first small torsion spring 117, the first card pushing seat lower 115 is arranged on the first sliding block 119, the first card pushing seat upper 114 is arranged above the first card pushing seat lower 115, the first small toothed plate 116 is arranged on the first card pushing seat lower 115, and the first synchronous belt mechanism 112 drives the first sliding block 119 to reciprocate, so that the first small push block 113 is driven to reciprocate.

As shown in fig. 1, 4, 27 and 30, a second card pushing mechanism 12 is provided on the side of the base plate 1 located at the first card pushing mechanism 11, a second timing belt mechanism 122 is mounted on the top of the base plate 1, a second motor 121 capable of driving the second timing belt mechanism 122 is mounted on the bottom of the base plate 1, the second card pushing mechanism 12 is mounted on the timing belt of the second timing belt mechanism 122, specifically, a second slider 129 is mounted on the timing belt, and the second slider 129 can follow the timing belt.

The second card pushing mechanism 12 comprises a second small push block 123, a second card pushing seat upper 124, a second card pushing seat lower 125, a second small toothed plate 126, a second small torsion spring 127 and a second stopper screw 128, wherein the second small torsion spring 127 is sleeved on the second stopper screw 128, the second small push block 123 is arranged on the second card pushing seat upper 124 through the second stopper screw 128 and the second small torsion spring 127, the second card pushing seat lower 125 is arranged on a second sliding block 129, the second card pushing seat upper 124 is arranged above the second card pushing seat lower 125, the second small toothed plate 126 is arranged on the second card pushing seat lower 125, and the second synchronous belt mechanism 122 drives the second sliding block 129 to reciprocate, so as to drive the second small push block 123 to reciprocate.

As shown in fig. 1, 4, and 28 to 32, a third push-clamping mechanism 13 is provided on the base plate 1 near the sample module 2, a third timing belt mechanism 132 is mounted on the top of the base plate 1, a third motor 131 capable of driving the third timing belt mechanism 132 is mounted on the bottom of the base plate 1, the third push-clamping mechanism 13 is mounted on the timing belt of the third timing belt mechanism 132, specifically, a third slider 139 is mounted on the timing belt, and the third slider 139 can follow the timing belt.

The third card pushing mechanism 13 comprises a third small pushing block 133, a third card pushing seat upper 134, a third card pushing seat lower 135, a third small toothed plate 136, a third small torsion spring 137, a third card pushing screw 138, a jacking block 48 and a cam block 49, wherein the third small torsion spring 137 is sleeved on the third card pushing screw 138, the third small pushing block 133 is installed on the third card pushing seat upper 134 through the third card pushing screw 138 and the third small torsion spring 137, the cam block 49 is installed on the third card pushing seat upper 134, the third card pushing seat lower 135 is installed on a third sliding block 139, the third card pushing seat upper 134 is installed above the third card pushing seat lower 135, the third small toothed plate 136 is installed on the third card pushing seat lower 135, and the third synchronous belt mechanism 132 drives the third small pushing block 133 to conduct reciprocating card pushing movement.

As shown in fig. 30, the reagent card 45 in the reagent cassette 43 is pushed into the rotary table 55 from the card pushing channel by the first card pushing mechanism 11, the reagent card 45 is pressed into the rotary table 55 by the pressing sheet 56, the rotary table 55 rotates to the card ejecting channel located beside the card pushing channel on the outermost side of the apparatus, and the second card pushing mechanism 12 pushes the reagent card 45 out of the rotary table 55.

As shown in fig. 30 to 32, when an emergency call is required, the reagent card 45 is inserted into an emergency call detection channel in the emergency call detection module 8 located beside the consumable module 3, a top block 48 is arranged on the emergency call detection channel, the top block 48 comprises a fourth spring 483, one end of the fourth spring 483 is installed on the cartridge clip supporting plate 41, the other end of the fourth spring is connected with a stop block 183, an L-shaped frame is installed at the bottom end of the stop block 183, and a pin 184 is installed on the L-shaped frame. The reagent card 45 is blocked by the stop block 183 at the initial position of the top block 48, the inductor 484 arranged on the cartridge clip supporting plate 41 senses the insertion of the reagent card 45, the third motor 131 starts to drive the third synchronous belt mechanism 132 to move, the cam block 49 of the third push card seat 134 on the third push card mechanism 13 moves along with the third sliding block 139 of the third push card mechanism 13 to push the pin 184 on the top block 48, the fourth spring 483 is pressed, the pin 184 is pushed to drive the stop block 183 to move, so that the reagent card 45 enters the push card channel of the third push card mechanism 13 from the channel of the emergency detection module 8, the third synchronous belt mechanism 132 continues to move, the small push block three 133 of the third push card mechanism 13 pushes the reagent card 45 into the turntable 55 under the driving, the rebound effect of the fourth spring 483 pushes the stop block 183 to move, the top block 48 returns to the initial position, the rotary power module 53 drives the turntable 55 to rotate to the card withdrawal channel, and the second push card mechanism 12 pushes the reagent card 45 out of the turntable 55. The second card pushing mechanism 12 pushes the reagent card 45 after incubation into the waste cartridge.

As shown in fig. 5, the cartridge holder supporting plate 41 is provided with a photoelectric data collecting module 6, the photoelectric data collecting module 6 includes a luminous box 61 and a reagent card pressing piece 62, before the second card pushing mechanism 12 pushes the card to the waste card box, the reagent card 45 passes through the photoelectric data collecting module 6, the reagent card pressing piece 62 presses the reagent card 45, and the luminous box 61 scans the reagent card 45 to collect data.

As shown in fig. 6 and 33-39, a sampling module 7 is disposed on the back surface of the consumable module 3 on the base plate 1. The sampling module 7 comprises an X-axis mechanical arm assembly 17, a Y-axis mechanical arm assembly 27, a Z-axis mechanical arm assembly 37 and a sampling assembly 47, the sampling module 7 can be displaced in a triaxial space, and the sampling assembly 47 of the sampling module 7 can suck and spit liquid. The X-axis mechanical arm assembly 17 comprises a first frame, a fourth motor 171 is installed at the bottom of the first frame, two first synchronous pulleys 175 are installed at the top of the first frame, the fourth motor 171 drives one of the first synchronous pulleys 175 to rotate, a fourth synchronous belt 172 is installed on the two first synchronous pulleys 175, a first synchronous sliding block is installed on the fourth synchronous belt 172, a first guide rail 173 and a second guide rail 174 are arranged at the top of the first frame, a fourth sliding block is installed on the first guide rail 173 and the second guide rail 174, and the fourth motor 171 drives the first synchronous pulleys 175 to move so that the mechanical arm can move on the first guide rail 173 and the second guide rail 174. The X-axis mechanical arm assembly 17 adopts a mode of double guide rails and synchronous belts, so that the mechanical arm can run more stably in the X-axis direction.

The Y-axis mechanical arm assembly 27 comprises a second frame, the second frame is mounted on the first synchronous slide block and the fourth slide block, the fourth motor 171 is driven to drive the first synchronous pulley 175 to rotate, the fourth synchronous belt 172 moves and simultaneously drives the second frame to move through the first synchronous slide block, and movement of the Y-axis mechanical arm assembly 27 in the X direction is achieved.

The Y-axis mechanical arm assembly 27 further includes two second timing pulleys 274 provided on the front side of the second frame, a fifth motor 271 provided on the rear side, the fifth motor 271 driving one of the second timing pulleys 274, a fifth timing belt 272 mounted on the two second timing pulleys 274, a third guide rail 273 provided on the front side of the second frame, and second timing sliders mounted on the fifth timing belt 272 and the third guide rail 273.

The Z-axis mechanical arm assembly 37 comprises a third frame, the third frame is mounted on the second synchronous slide block, the fifth motor 271 drives the second synchronous pulley 274 to rotate, and the fifth synchronous belt 272 drives the third frame to move by driving the second synchronous slide block while moving, so that the movement of the Z-axis mechanical arm assembly 37 in the Y direction is realized.

The Z-axis mechanical arm assembly 37 further includes two third timing pulleys 374 disposed on the front side of the third frame, a sixth motor 371 disposed on the rear side, the sixth motor 371 driving one of the third timing pulleys 374, a sixth timing belt 372 mounted on the two third timing pulleys 374, a fourth guide rail 373 disposed on the front side of the third frame, and third timing sliders mounted on the sixth timing belt 372 and the fourth guide rail 373.

The sampling assembly 47 is mounted on the third synchronous slide block, the sixth synchronous belt 372 drives the third synchronous pulley 374 to rotate, the sixth synchronous belt 372 drives the sampling assembly 47 by driving the third synchronous slide block while moving, and the movement of the sampling assembly 47 in the Z direction is achieved, specifically, the sampling assembly 47 is connected with the third synchronous slide block through a connecting piece. The lower end of the X-axis mechanical arm assembly 17 of the sampling module 7 is provided with an injector assembly 178, the injector assembly 178 can be used for sucking samples, and the Y, Z mechanical arm assembly adopts a transmission mode of a guide rail and a synchronous belt so that the mechanical arm can operate more efficiently and stably in a Z-axis.

As shown in fig. 36 to 39, the sampling assembly 47 includes an upper case 471, a middle case 472, a lower case 473, a sleeve 474, a small spring 475, a needle 476, a TIP head presence sensor 477, a connecting shaft 478, a connecting block 479, a connecting block 480, a connecting small shaft 481, and a TIP head 34. Connecting shaft 478 is connected with connecting block 479, connecting block 480 is fixed on connecting block 479, TIP head presence/absence sensor 477 is fixed on connecting block 480, connecting small shaft 481 is connected with connecting block 479, small spring 475 is sleeved on connecting small shaft 481, sleeve 474 is connected with connecting small shaft 481 again, lower shell 473 and middle shell 472 are assembled with upper shell 471 from bottom to top and cover other components except sleeve 474, needle 476 and connecting small shaft 481, and needle 476 is assembled with connecting small shaft 481.

The TIP head 34 is inserted into the sampling assembly 47, and the further TIP head presence sensor 477 detects that the TIP head 34 has been taken, and the sampling assembly 47 draws the sample and diluent from the sample module 2 and moves it onto the mixing tray 33 for mixing and adding it to the reagent card 45 to be incubated. During the process of picking up the TIP head 34 by the sampling assembly 47, the sampling assembly 47 moves downward, the needle 476 is inserted into the TIP head 34, at this time, the small spring 475 of the sampling assembly 47 contracts, the TIP head 34 is picked up, and whether the TIP head has the sensor 477 detects the TIP head 34; the sampling assembly 47 moves upward, at which point the small spring 475 of the sampling assembly 47 returns, driving the needle 476 to return. After mixing, the TIP head 34 is discarded, the sampling assembly 47 moves downward, the TIP head 34 moves into the TIP discarding nozzle 35, at this time, the TIP head 34 is located at a large caliber of the TIP discarding nozzle 35, the TIP head 34 moves into a small caliber of the TIP discarding nozzle 35 in the TIP discarding nozzle 35, and the TIP head 34 is clamped in a small caliber of the TIP discarding nozzle 35 during the upward movement of the sampling assembly 47, so that the TIP head 34 is discarded. Needle 476 is then moved upward to discard TIP 34 through TIP discard nozzle 35 to complete replacement of TIP 34, TIP presence sensor 477 detects that TIP 34 has been discarded, small spring 475 resets sleeve 474, and sampling assembly 47 may repeatedly aspirate fluid.

Go up shell cover 471, well shell cover 472, lower shell cover 473 adoption disconnect-type structural design, and limit function about and to the sleeve has been realized in the time of easy to assemble, and the structural design of sleeve 474 has more convenient realization and has detected the function that the TIP head exists, and this structural design's advantage lies in its function of having realized sampling assembly 47 with clearer structure.

As shown in fig. 7 and 32, the emergency detection module 8 is disposed beside the cartridge clip module 4 on the bottom plate 1, the reagent card 45 can be directly plugged into the cartridge clip module 8 from the emergency detection module 8, the scanner port 185 scans the information of the reagent card 45, the inductor 484 disposed on the cartridge clip support plate 41 senses the insertion of the reagent card 45, the third motor 131 starts to drive the third synchronous belt mechanism 132 to move, the cam block 49 of the third push card seat 134 on the third push card mechanism 13 moves along with the third slider 139 of the third push card mechanism 13, the pin 184 on the push top block 48 is pressed, the pin 184 is driven to move by the fourth spring 483, the reagent card 45 enters the push card channel of the third push card mechanism 13 from the channel of the emergency detection module 8, the third synchronous belt mechanism 132 continues to rotate, the small push block three 133 of the third push card mechanism 13 pushes the reagent card 45 into the turntable 55 under the driving action of the rebound of the fourth spring 483, the push block 183 moves, the top block 48 returns to the initial position, the rotating power module 53 drives the turntable 55 to rotate the card 55 to rotate the second push the reagent card 45 back into the second optical data collection channel 12 through the optical data collection device 55, and the data collection device is completed after the second push card 45 is pushed back into the optical data collection device 12.

As shown in fig. 40, the present invention is provided with a casing 100 outside, a hatch door 102 is provided on the casing 100, the hatch door 102 is provided outside the sample module 2 and the consumable module 3, and the sample or TIP head disk 32 and the mixing disk 33 can be replaced by opening the hatch door 102. The display screen 101 is arranged above the cabin door 102, the display screen 101 is electrically connected with the first sensor 211 and the photoelectric data acquisition module 6 on the rear baffle of the blood sampling frame 21, one-dimensional codes scanned by the detector and information acquired by the photoelectric data acquisition module 6 can be transmitted into a control system in the display screen 101 in real time, controllable sample information, positions, types, incubation time and incubation types can be displayed on the interface of the display screen 101, and the incubation can be performed in a grouping and classifying mode.

As shown in fig. 41, an immunoassay detection method comprising the steps of:

s1, sliding guide blocks of a blood sampling frame 21 and a diluent frame 22 in a sample module 2 into guide rail grooves on a supporting plate 23, and automatically inputting one-dimensional code information on the blood sampling frame 21 by a code scanner arranged on a bottom plate 1 when the first sensor 211 arranged on a baffle plate at the rear side of the position of the blood sampling frame 21 senses the insertion of the blood sampling frame 21 so as to judge the detection sample position and patient information of the blood sampling frame 21.

S2, aligning the side edge guide grooves 451 of the reagent card 45 with the protruding ribs of the two guide tables, sequentially loading the reagent card 45 into the reagent kit 43, and inserting the reagent kit 43 into the reagent kit channel 46 from the clip baffle notch structure 461 on the clip baffle 44 of the clip module 4.

S3, after the detection item information is recorded, a fourth motor 171 of the X-axis mechanical arm assembly 17 drives a first synchronous pulley 175 to move so as to drive a fourth sliding block and a first synchronous sliding block on the X-axis mechanical arm assembly 17 to move on a first guide rail 173 and a second guide rail 174, namely, the Y-axis mechanical arm assembly 27 moves on the X-axis, a fifth motor 271 of the Y-axis mechanical arm assembly 27 drives a second synchronous pulley 274 to move so as to drive a second synchronous sliding block on the Y-axis mechanical arm assembly 27 to move on a third guide rail 273, namely, the Z-axis mechanical arm assembly 37 moves on the Y-axis, the sixth motor 371 of the Z-axis mechanical arm assembly 37 drives the third synchronous pulley 374 to move to drive the third synchronous sliding block on the Z-axis mechanical arm assembly 37 to move on the fourth guide rail 373, namely, the sampling assembly 47 moves up and down on the Z-axis, the mechanical arm assembly moves in three-dimensional space to drive the sampling assembly 47 to move to the position of the TIP head disc 32 at a preset position, the sampling assembly 47 moves downwards, the needle 476 is inserted into the TIP head 34, at the moment, the small spring 475 of the sampling assembly 47 contracts, the TIP head 34 is picked up and finished, and the TIP head has or not a sensor 477 detects the TIP head 34; the sampling assembly 47 moves upwards, at this time, the small spring 475 of the sampling assembly 47 resets, the needle 476 is driven to reset, the mechanical arm assembly drives the sampling assembly 47 to move above the blood sampling frame 21 of the sample module 2, the sixth motor 371 drives the third synchronous pulley 374 to move, so that the sampling assembly 47 moves downwards on the fourth guide rail 373 to suck samples, and then the samples are moved to the same action above the diluent frame 22 to suck diluent. The mechanical arm assembly drives the sampling assembly 47 to move above the mixing disk 33 in the XY direction, and the Z-axis mechanical arm assembly 37 drives the sampling assembly 47 to move downwards so that the sample and the diluent are mixed on the mixing disk 33.

S4, the reagent card 45 in the reagent box 43 in the cartridge clip module 4 slides downwards along a guide table in the reagent box 43 and enters a card pushing channel from a reagent card outlet structure, a first motor 111 of the first card pushing mechanism 11 drives a first synchronous belt mechanism 112 to drive a first sliding block 119 to reciprocate, and then drives a small pushing block one 113 to reciprocate, and the small pushing block one 113 hooks a notch below the reagent card 45 to push the reagent card 45 to enter the turntable 55; at this time, the mechanical arm assembly of the sampling module 7 drives the sampling assembly 47 to suck the uniformly mixed sample, and moves the uniformly mixed sample onto the reagent card 45 in the turntable 55 according to a predetermined direction, and the uniformly mixed sample is added into the reagent card 45 for reaction.

S5, after the mixing is completed, the mechanical arm assembly drives the sampling assembly 47 to move above the discarding device of the consumable module 3 in the XY axis direction, the sampling assembly 47 moves downwards to move the TIP head 34 into the TIP discarding nozzle 35, at this time, the TIP head 34 is positioned at the large caliber of the TIP discarding nozzle 35, the TIP head 34 moves into the small caliber of the TIP discarding nozzle 35 in the TIP discarding nozzle 35, and the TIP head 34 is clamped in the small caliber of the TIP discarding nozzle 35 to complete the discarding of the TIP head 34 in the upward moving process of the sampling assembly 47. Needle 476 is then moved upward to discard TIP 34 through TIP discard nozzle 35 to complete replacement of TIP 34, TIP presence sensor 477 detects TIP 34 has been discarded, small spring 475 resets sleeve 474, and sampling assembly 47 may repeatedly aspirate liquid discharge.

S6, after the reaction is finished, the rotary table 55 rotates to a card withdrawing channel, a second motor 121 in the second card pushing mechanism 12 arranged on the bottom plate 1 drives a second synchronous belt mechanism 122 to move to drive a second sliding block 129 to reciprocate to push a card, and then drives a small pushing block II 123 to reciprocate, the small pushing block II 123 hooks a notch below the reagent card 45 to push the reagent card 45 into the photoelectric data acquisition module 6, a reagent card sheet 62 of the photoelectric data acquisition module 6 on the cartridge clip supporting plate 41 presses the reagent card 45, the luminous box 61 performs data acquisition and completes detection, and after the detection is finished, a second motor 121 in the second card pushing mechanism 12 arranged on the bottom plate 1 drives a second synchronous belt mechanism 122 to move to drive a second sliding block 129 to reciprocate to push the card, and then drives the small pushing block II 123 to reciprocate, and the small pushing block II 123 hooks a notch below the reagent card 45 to push the reagent card 45 out, and pushes the reagent card 45 from the rotary table 55 to a waste box.

As shown in fig. 42, an emergency condition;

s11, placing the blood collection tube 36 into the emergency treatment frame 31, placing the emergency treatment sample and the diluent into the mixing disc 33 by the sampling assembly 47 for uniform mixing, and inserting the reagent card 45 into the channel of the emergency treatment detection module 8.

S22, an inductor 484 arranged on the cartridge clip supporting plate 41 senses the insertion of the reagent card 45, a third motor 131 starts to drive a third synchronous belt mechanism 132 to move, a cam block 49 of a third push card seat 134 on the third push card mechanism 13 moves along with a third sliding block 139 of the third push card mechanism 13, a pin 184 on a top block 48 is pushed, a fourth spring 483 is pressed, the pin 184 is pushed to drive a stop block 183 to move away, the reagent card 45 enters a push card channel of the third push card mechanism 13 from a channel of the emergency detection module 8, the third synchronous belt mechanism 132 continues to rotate, a small push block three 133 of the third push card mechanism 13 pushes the reagent card 45 into a turntable 55 to perform incubation, the stop block 183 moves under the rebound action of the fourth spring 483, the top block 48 returns to an initial position, a rotary power module 53 drives the turntable 55 to rotate, and the sampling module 7 adds an emergency mixed sample into the reagent card 45 to perform reaction.

And S33, rotating the turntable 55 to a card withdrawing channel after incubation time, pushing the reagent card 45 into the photoelectric data acquisition module 6 by the second card pushing mechanism 12, and pushing the reagent card 45 into a waste card box by the second card pushing mechanism 12 after incubation is completed after data acquisition and detection are completed by the photoelectric data acquisition module 6.

The invention has the technical effects that the immunoassay detection equipment and the detection method solve the problem that the instrument automation is difficult to realize, and simultaneously solve the problem that the existing high-automation equipment needs to detect simultaneously in more items, and provide the immunoassay analyzer which can detect various samples simultaneously, has high module integration level and accurate and reliable detection results, and in addition, the emergency detection module and the emergency frame are added to increase the detection mobility when the emergency is dealt with.

The invention is intended to cover any alternatives, modifications, equivalents, and variations that fall within the spirit and scope of the invention. In the following description of preferred embodiments of the invention, specific details are set forth in order to provide a thorough understanding of the invention, and the invention will be fully understood to those skilled in the art without such details. In other instances, well-known methods, procedures, flows, components, circuits, and the like have not been described in detail so as not to unnecessarily obscure aspects of the present invention.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims

1. The immunoassay detection equipment is characterized by comprising a shell, wherein the shell comprises a bottom plate, and a sample module, a consumable module, a cartridge clip module, an incubation module, a photoelectric data acquisition module, a sampling module and an emergency treatment detection module are sequentially integrated and installed in the shell;

the sampling module is arranged behind the consumable module on the bottom plate; the sampling module comprises a sampling assembly, an X-axis mechanical arm assembly, a Y-axis mechanical arm assembly and a Z-axis mechanical arm assembly, wherein the X-axis mechanical arm assembly, the Y-axis mechanical arm assembly and the Z-axis mechanical arm assembly can drive a sampled sample X, Y, Z to move in the direction;

a sample module and a consumable module are installed in the shell; the sample module comprises a supporting plate, a guide rail groove is formed in the supporting plate, a blood sampling frame and a diluent frame are inserted into the guide rail groove, and a notch structure is formed in the insertion position of the guide rail groove; the back of the supporting plate is provided with a back plate, the back plate is provided with a collision bead, the tail ends of the blood sampling frame and the diluent frame are respectively provided with collision bead grooves, and a first sensor is arranged on a rear baffle of the blood sampling frame;

The consumable module comprises a consumable body, wherein a TIP head supporting plate and a mixing disc are arranged at the top of the consumable body, a TIP head disc is arranged above the TIP head supporting plate, an emergency treatment frame and a TIP discarding device are arranged on the side face of the consumable body, a TIP discarding nozzle on the TIP discarding device adopts a gradual change structure, and the upper part of the emergency treatment frame adopts a notch structure and variable diameter treatment;

the cartridge clip module comprises a cartridge clip supporting plate, and at least one group of cartridge clip baffle plates are arranged on the cartridge clip supporting plate; a reagent box is inserted between each group of cartridge clip baffle plates;

the outlet of the lower end of the reagent kit is provided with a reagent card outlet structure, the reagent card outlet structure comprises a torsion spring and a reagent card outlet structure baffle, and the torsion spring is respectively connected with the inner wall of the reagent kit and the reagent card outlet structure baffle;

the incubation module comprises an upper heat preservation cover, a lower heat preservation cover, a rotary power module and a second sensor, wherein the second sensor is arranged on the upper heat preservation cover, the rotary power module is arranged below the heat preservation cover, and the rotary power module comprises a turntable and a turntable motor;

the rotary table is provided with a pressing sheet, a connecting main shaft is connected below the rotary table, a bearing sleeve is connected below the bottom plate, the connecting main shaft is fixed on a bearing, a bearing inner ring is provided with the connecting main shaft, and a bearing outer ring is provided in the bearing sleeve; a turntable synchronous large belt wheel and an expansion sleeve are sequentially installed on the connecting main shaft, a motor installation seat is installed on the bottom plate, the turntable motor is installed on the motor installation seat, a turntable synchronous small belt wheel is installed on an output shaft of the turntable motor, and turntable synchronous belts are installed on the turntable synchronous large belt wheel and the turntable synchronous small belt wheel;

The consumable module is provided with a TIP head, a TIP discarding nozzle with a gradual change structure and an emergency treatment frame, and emergency treatment blood sampling samples can be placed on the emergency treatment frame; the sampling module can pick up or discard the TIP head, pick up the blood sampling sample and the diluent in the sample module, mix the blood sampling sample and the diluent uniformly, and add the mixed sample to the reagent card in the incubation module for reaction; the cartridge clip module is internally provided with the reagent card, and the reagent card for emergency treatment can be inserted into the emergency treatment detection module; a first card pushing mechanism in the incubation module pushes the reagent card in the cartridge clip module into the incubation module; the sensor senses that the reagent card for emergency treatment is inserted into the emergency treatment detection module, and a third card pushing mechanism in the incubation module pushes the reagent card for emergency treatment into the incubation module; a second card pushing mechanism in the incubation module pushes the incubated reagent card to a waste card box;

the sampling module comprises a sampling assembly, wherein the sampling assembly comprises a needle head for picking up or discarding the TIP head and a TIP head presence sensor for detecting whether the TIP head is arranged on the needle head;

the bottom plate is provided with a third synchronous belt mechanism close to the sample module, the top of the bottom plate is provided with a third motor capable of driving the third synchronous belt mechanism, the synchronous belt of the third synchronous belt mechanism is provided with the third card pushing mechanism, a third sliding block is arranged on the synchronous belt and can move along with the synchronous belt, the lower end of the third card pushing mechanism is arranged on the third sliding block, and the third synchronous belt mechanism drives the third sliding block to carry out reciprocating card pushing movement;

The third pushing and clamping mechanism comprises a third small pushing block, a third pushing and clamping seat, a lower part of the third pushing and clamping seat, a third small toothed plate, a third small torsion spring, a third plugging and screwing screw, a jacking block and a cam block, wherein the third small torsion spring is sleeved on the third plugging and screwing screw, the third small pushing block is arranged on the third pushing and clamping seat through the third plugging and screwing screw and the third small torsion spring, the cam block is arranged on the third pushing and clamping seat, the lower part of the third pushing and clamping seat is arranged on a third sliding block, the upper part of the lower part of the third pushing and clamping seat is arranged on the lower part of the third pushing and clamping seat, and the third small toothed plate is arranged on the lower part of the third pushing and clamping seat;

the reagent card for emergency treatment is inserted into an emergency treatment detection channel in the emergency treatment detection module beside the consumable module, a top block is arranged on the emergency treatment detection channel, the top block comprises a fourth spring, one end of the fourth spring is arranged on a cartridge clip supporting plate, the other end of the fourth spring is connected with a stop block, the bottom end of the stop block is provided with an L-shaped frame, and the L-shaped frame is provided with a pin; the cartridge clip supporting plate is provided with an inductor for inducing the reagent card of the emergency to be inserted;

when an emergency call is needed, the reagent card is inserted into an emergency call detection channel in an emergency call detection module beside a consumable module, the reagent card is blocked by a stop block at the initial position, an inductor arranged on a cartridge clip supporting plate senses the insertion of the reagent card, a third motor starts to drive a third synchronous belt mechanism to move, a cam block on a third card pushing seat on the third card pushing mechanism moves along with a third sliding block of the third card pushing mechanism to push a pin on the top block, a fourth spring is pressed, the pin is pushed to drive the stop block to move away, the reagent card enters a card pushing channel of the third card pushing mechanism from the channel of the emergency call detection module, the third synchronous belt mechanism continues to move, a small push block III of the third card pushing mechanism pushes the reagent card into a rotary table under the driving of the third spring, the stop block is pushed to move under the rebound action of the fourth spring, the rotary power module drives the rotary table to rotate, and the rotary table rotates to the card withdrawing channel, and the second card pushing mechanism pushes the reagent card out of the rotary table; the second card pushing mechanism pushes the incubated reagent card into the waste card box.

2. The immunoassay detection instrument according to claim 1, wherein four first pushing and clamping mechanisms are arranged on the bottom plate corresponding to the reagent box channel, a first synchronous belt mechanism is arranged at the top of the bottom plate, a first motor for driving the first synchronous belt mechanism is arranged at the bottom of the bottom plate, the first pushing and clamping mechanism is arranged on a synchronous belt of the first synchronous belt mechanism, a first sliding block is arranged on the synchronous belt, the first sliding block can move along with the synchronous belt, the lower end of the first pushing and clamping mechanism is arranged on the first sliding block, and the first synchronous belt mechanism drives the first sliding block to do reciprocating pushing and clamping movement;

the side that is located on the bottom plate first card pushing mechanism is provided with second card pushing mechanism the second hold-in range mechanism is installed at the top of bottom plate the bottom of bottom plate is installed the drive second motor of second hold-in range mechanism install on the hold-in range of second hold-in range mechanism second card pushing mechanism installs on the hold-in range, the second slider can follow the hold-in range motion, the lower extreme of second card pushing mechanism is installed on the second slider, second hold-in range mechanism drives the second slider carries out reciprocal card pushing motion.

3. The immunoassay detection instrument of claim 1, wherein the X-axis mechanical arm assembly comprises a first frame, a fourth motor is mounted at the bottom of the first frame, two first synchronous pulleys are mounted at the top of the first frame, the fourth motor drives one of the first synchronous pulleys to rotate, a fourth synchronous belt is mounted on the two first synchronous pulleys, a first synchronous slider is mounted on the fourth synchronous belt, a first guide rail and a second guide rail are arranged at the top of the first frame, a fourth slider is mounted on the first guide rail and the second guide rail, and the Y-axis mechanical arm assembly is mounted on the fourth slider and the first synchronous slider;

the Y-axis mechanical arm assembly comprises a second rack, and the second rack is arranged on the first synchronous slide block and the fourth slide block; two second synchronous pulleys are arranged on the front side surface of the second frame, a fifth motor is arranged on the rear side surface of the second frame, one of the second synchronous pulleys is driven by the fifth motor, a fifth synchronous belt is arranged on the two second synchronous pulleys, a third guide rail is arranged on the front side surface of the second frame, second synchronous sliding blocks are arranged on the fifth synchronous belt and the third guide rail, and the Z-axis mechanical arm assembly is arranged on the second synchronous sliding blocks;

The Z-axis mechanical arm assembly comprises a third rack, and the third rack is mounted on the second synchronous slide block; two third synchronous pulleys are arranged on the front side surface of the third frame, a sixth motor is arranged on the rear side surface of the third frame, one of the third synchronous pulleys is driven by the sixth motor, a sixth synchronous belt is arranged on the two third synchronous pulleys, a fourth guide rail is arranged on the front side surface of the third frame, a third synchronous sliding block is arranged on the sixth synchronous belt and the fourth guide rail, and the sampling assembly is arranged on the third synchronous sliding block.

4. The immunoassay detection instrument of claim 3, wherein the sampling assembly comprises a connecting shaft, the top of the connecting shaft is mounted on the third synchronous slide block through a connecting piece, the connecting shaft is connected with a connecting block, a connecting block is fixed on the connecting block, a TIP head sensor is fixed on the connecting block, the connecting block is connected with the connecting block, a small spring is sleeved on the connecting block, a sleeve is connected with the connecting block, a middle shell cover and a lower shell cover are mounted with an upper shell cover from bottom to top and cover other components except the sleeve, a needle head and the connecting block, and the needle head is mounted with the connecting block.

5. The immunoassay detection instrument of claim 1, wherein the housing comprises a base plate and a housing, wherein a door is provided on the housing, the door is provided outside the sample module and the consumable module, a display screen is provided above the door, and the display screen is electrically connected with the first sensor of the rear baffle of the blood collection rack and the optoelectronic data acquisition module.

6. An immunoassay detection method comprising an immunoassay detection device according to any one of claims 1 to 5, wherein the detection method comprises a collective detection and an emergency detection; the collective detection includes:

the emergency detection includes:

after incubation is completed, the turntable is turned to a card returning channel, the second card pushing mechanism pushes the reagent card of the emergency to the photoelectric data acquisition module, the photoelectric data acquisition module acquires information and transmits the information to the control system, and the second card pushing mechanism pushes the reagent card of the emergency after incubation to the waste card box.