CN114813300A

CN114813300A - Blood sample diluting device and diluting method for immunoassay detection equipment

Info

Publication number: CN114813300A
Application number: CN202210720460.6A
Authority: CN
Inventors: 李瑞庆; 郭永峰; 王陈; 余占江; 程小雷; 陈旭; 句江鹏; 张少康
Original assignee: Pro Med Beijing Technology Co ltd
Current assignee: Pro Med Beijing Technology Co ltd
Priority date: 2022-06-24
Filing date: 2022-06-24
Publication date: 2022-07-29

Abstract

The invention provides a blood sample diluting device and a diluting method for immunoassay detection equipment, belonging to the technical field of medical auxiliary equipment; the system comprises a sampling module, a consumable module and a sample module, wherein the sampling module comprises a sampling assembly, an X-axis mechanical arm assembly, a Y-axis mechanical arm assembly and a Z-axis mechanical arm assembly which can drive the sampling assembly X, Y, Z to move in the direction; the consumable module is provided with an emergency treatment frame and a TIP mouth discarding device. The sampling module mechanical arm can solve the problem of human-computer interaction between a doctor and a machine in sample placement, improves the use experience and convenience of the instrument, improves the convenience of the instrument for emergency situations by adding an emergency frame to a consumable module, improves the discarding efficiency by adopting a gradient gap design for a TIP discarding mouth, can realize the sample adding function with lower cost by adopting a guide rail and synchronous belt motion mode compared with the traditional mechanical arm adopting a spline shaft connection mode, and improves the sampling efficiency and the discarding efficiency by arranging a TIP head or not.

Description

Blood sample diluting device and diluting method for immunoassay detection equipment

Technical Field

The invention relates to the technical field of medical auxiliary equipment, in particular to a blood sample diluting device and a diluting method for immunoassay detection equipment.

Background

The immunoassay is an analysis technique based on characteristic reactions of antigens and antibodies, and is mainly classified into radioimmunoassay, enzyme immunoassay, chemiluminescence immunoassay, fluorescence immunoassay and the like according to different labeling techniques.

In the operation process of the existing immunoassay detection equipment, after a detection mode is set, the immunoassay detection equipment carries out detection analysis in the whole process according to a preset sequence, if an emergency blood sample needing urgent analysis exists, the immunoassay detection equipment can analyze the emergency blood sample after the detection analysis is finished, the immunoassay detection equipment is deficient in mobility and cannot cope with emergency situations of emergency treatment, and the existing immunoassay detection equipment does not have an emergency frame. After the existing blood sampling frame is inserted into the sampling mixing module, the positioning between the blood sampling frame and the sampling mixing module can not be realized; the discarding device of the consumable module in the prior art usually adopts the design of a common notch, so that the phenomenon of blocking is easy to occur in the discarding process, and the consumable replacement efficiency is reduced.

In the operation process of the existing immunoassay detection equipment, after a detection mode is set, the immunoassay detection equipment carries out detection analysis in the whole process according to a preset sequence, if an emergency blood sample needing urgent analysis exists, the immunoassay detection equipment can analyze the emergency blood sample after the detection analysis is finished, the immunoassay detection equipment is deficient in mobility and cannot cope with emergency situations of emergency treatment, and the existing immunoassay detection equipment does not have an emergency detection channel.

When a blood sample detection analyzer is used for detecting a blood sample, the blood sample needs to be transferred from one station to another station, for example, the sample in the sample plate assembly is conveyed to a detection assembly for detection, and after the detection is finished, the TIP head containing the sample is conveyed to a consumable automatic separation mechanism for realizing automatic separation; therefore, the driving mechanism is very important for immunoassay; the actuating mechanism that different immunoassay appearance used is different, and actuating mechanism can not realize generally, and current mode is artifical to adsorb the blood sample through the instrument and react on the reaction carrier, needs artifical manually operation, and the blood sample volume of absorption is not controlled well, needs to absorb many times, and efficiency is lower.

When the blood sample is analyzed, the blood sample needs to be sucked and then transferred to a detection assembly for blood sample detection and analysis, the blood sample is generally transferred by TIP, and after the blood sample is transferred to the detection assembly for detection, the TIP head needs to be manually removed, so that the operation is inconvenient; therefore, a mechanism for automatically releasing the consumable material is needed.

Disclosure of Invention

The technical problem to be solved by the invention is to provide a blood sample diluting device and a diluting method for immunoassay detection equipment, which solve the problems of poor emergency call maneuverability, inconvenience in equipment use and inconvenience in sampling of the equipment, can automatically absorb and mix a sample, can automatically separate and discard a collecting tool after the mixing is finished, and improve the working efficiency of the equipment.

In order to solve the technical problems, the invention provides the following technical scheme:

a blood sample diluting device for immunoassay detection equipment comprises a sampling module, a consumable module and a sample module, wherein the sampling module comprises a sampling assembly, an X-axis mechanical arm assembly, a Y-axis mechanical arm assembly and a Z-axis mechanical arm assembly which drive the sampling assembly X, Y, Z to move directionally;

the sample module comprises a support plate, wherein a guide rail groove is formed in the support plate, a blood sampling frame and a diluent frame are inserted into the guide rail groove, and an opening structure is formed in the insertion position of the guide rail groove; the back of the supporting plate is provided with a back plate, the back plate is provided with a collision bead, the tail ends of the blood sampling frame and the diluent frame are respectively provided with a collision bead groove, and the rear side of the blood sampling frame is provided with a first sensor;

the consumable module comprises a consumable body, an emergency treatment frame and a TIP discarding device are arranged on the side face of the consumable body, a TIP discarding nozzle on the TIP discarding device is of a gradual change type structure, and the upper part of the emergency treatment frame is of a notch type structure and is subjected to reducing treatment;

the sampling assembly includes a needle to pick up or discard a TIP and a TIP presence sensor to detect whether a TIP is present on the needle.

Preferably, the X-axis mechanical arm assembly comprises a first frame, a fourth motor is installed at the bottom of the first frame, two first synchronous belt pulleys are installed at the top of the first frame, the fourth motor drives one of the first synchronous belt pulleys to rotate, a fourth synchronous belt is installed on the two first synchronous belt pulleys, a first synchronous sliding block is installed on the fourth synchronous belt, a first guide rail and a second guide rail are arranged at the top of the first frame, a fourth sliding block is installed on the first guide rail and the second guide rail, and the Y-axis mechanical arm assembly is installed on the fourth sliding block and the first synchronous sliding block.

Preferably, the Y-axis robot arm assembly comprises a second frame mounted on the first and fourth synchronization sliders; the Z-axis mechanical arm assembly is characterized in that two second synchronous belt wheels are arranged on the front side face of the second rack, a fifth motor is arranged on the rear side face of the second rack, the fifth motor drives one of the second synchronous belt wheels, fifth synchronous belts are installed on the two second synchronous belt wheels, a third guide rail is arranged on the front side face of the second rack, second synchronous sliding blocks are installed on the fifth synchronous belts and the third guide rail, and the Z-axis mechanical arm assembly is installed on the second synchronous sliding blocks.

Preferably, the Z-axis robot arm assembly comprises a third frame mounted on a second synchronization slide; be provided with the sixth motor on two third synchronous pulleys, the trailing flank on the leading flank of third frame, sixth motor drive one of them third synchronous pulley is two install the sixth hold-in range on the third synchronous pulley be provided with the fourth guide rail on the leading flank of third frame the sixth hold-in range with install the third synchronous slide block on the fourth guide rail install on the third synchronous slide block the sampling subassembly.

Preferably, the sampling assembly comprises a connecting shaft, the connecting shaft is connected with a connecting block, a connecting small block is fixed on the connecting block, a TIP head is fixed on the connecting small block, the connecting small shaft is connected with the connecting block, a small spring sleeve is sleeved on the connecting small shaft, a sleeve is connected on the connecting small shaft, a lower shell cover and a middle shell cover are assembled with an upper shell cover from bottom to top and cover other components except the sleeve, a needle head and the connecting small shaft, and the needle head is assembled with the connecting small shaft.

Preferably, the TIP disposal nozzle of the tapered structure comprises a large-diameter portion and a small-diameter portion, and the large-diameter portion and the small-diameter portion are in transitional communication.

Preferably, a TIP head support plate and a mixing disc are arranged at the top of the consumable body, and a TIP head disc is arranged above the TIP head support plate.

Preferably, two clamping pieces are respectively arranged on two sides of the TIP head disc, the clamping pieces adopt two-section bending structures, and the two sides simultaneously clamp the TIP head supporting plate inwards to finish the fixation of the TIP head supporting plate and the TIP head disc.

Preferably, the blood sampling frame with the diluent frame all is equipped with the pushing hands, and the bottom of pushing hands is equipped with indent formula curved surface structure, be equipped with heparin tube installation unit on the blood sampling frame, heparin tube installation unit is open type structure.

A blood sample diluting method of an immunoassay testing device comprises the blood sample diluting device for the immunoassay testing device, and is characterized in that the blood sample diluting method comprises a collective blood sample diluting method and an emergency blood sample diluting method;

the collective blood sample dilution method comprises the following steps:

inserting a blood sampling frame in a sample module into the sample module, detecting insertion information of the blood sampling frame by a first sensor, transmitting the information to a control system, and automatically inputting one-dimensional code information on the blood sampling frame;

after one-dimensional code information is input, the control system controls the sampling module sampling assembly to sequentially absorb a blood sampling sample and diluent, the blood sampling sample and the diluent are added into a mixing disc to be mixed uniformly, and the sampling module adds the mixed sample into a reagent card to be incubated;

the emergency blood sample dilution method comprises the following steps:

placing the blood collection tube into an emergency treatment frame;

control system control sampling module, sampling component absorb emergency call blood sampling sample and diluent and put into with it carry out the mixing in the mixing dish, the reagent card of emergency call is sensed to the inductor, the sample after the sampling module adds the sample after the mixing and incubate in the reagent card of emergency call.

Compared with the prior art, the invention at least has the following beneficial effects:

among the above-mentioned scheme, a sample module that is arranged in immunoassay check out test set's blood specimen diluting device adopts the guide rail pull design can solve doctor and machine at the human-computer interaction problem that the sample was placed, has promoted the experience sense and the convenience that the instrument used, and the design of guide rail makes blood sampling frame pull more convenient, and the consumptive material module has added the emergency call frame and has promoted the convenience that the instrument dealt with the emergency call condition and TIP abandons the mouth and adopts the design of gradual change opening to promote and abandon efficiency.

The invention changes the connection mode of the transmission spline shaft of the mechanical arm, thereby realizing the sample adding function of the mechanical arm of the analytical instrument in a lower-cost and more stable motion mode of the guide rail and the synchronous belt; the sampling assembly is provided with the TIP existence device, so that the existence of the TIP of the sampling device can be detected more efficiently and automatically, the trouble of manual replacement is avoided, and the TIP head of the sampling assembly can be automatically separated from the sampling assembly, so that the discarding efficiency is increased.

Drawings

The accompanying drawings, which are incorporated herein and form a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the disclosure and to enable a person skilled in the pertinent art to make and use the disclosure.

FIG. 1 is a schematic view of the immunoassay test device of the present invention;

FIG. 2 is a schematic diagram of a sampling mixing module of the immunoassay testing apparatus of the present invention;

FIG. 3 is a first schematic structural diagram of a cartridge module of the immunoassay detection apparatus of the present invention;

FIG. 4 is a schematic structural diagram of a card pushing mechanism and an incubation module of the immunoassay detection device of the present invention;

FIG. 5 is a schematic view of an optoelectronic data acquisition module of the immunoassay test device of the present invention;

FIG. 6 is a schematic structural diagram of a sampling module of the immunoassay testing device of the present invention;

FIG. 7 is a schematic diagram of the emergency detection module of the immunoassay detection device of the present invention;

FIG. 8 is a schematic view showing the construction of a blood collection holder and a diluent holder of the immunoassay test device of the present invention;

FIG. 9 is a schematic view of the structure of the support plate of the sample block of the immunoassay test device of the present invention;

FIG. 10 is a schematic view of a bead-on-bead connection of a sample block of the immunoassay test device of the present invention;

FIG. 11 is a schematic view of a first sensor of the sample module of the immunoassay test device of the present invention;

FIG. 12 is a schematic structural diagram of a consumable module of the immunoassay test device of the present invention;

FIG. 13 is a schematic view of an emergency treatment device of the consumable module of the immunoassay test device of the present invention;

FIG. 14 is a schematic view of the TIP disposal nozzle of the consumable module of the immunoassay test device of the present invention;

FIG. 15 is a first schematic view of the clamping sheet of the consumable module of the immunoassay test device of the present invention;

FIG. 16 is a second schematic structural view of the clamping sheet of the consumable module of the immunoassay test device of the present invention;

FIG. 17 is a schematic view of the structure of the lower cover of the reagent cartridge of the immunoassay detection device of the present invention;

FIG. 18 is a detailed schematic view of FIG. 17;

FIG. 19 is a schematic view showing the structure of the upper cover of the reagent cartridge of the immunoassay detection device of the present invention;

FIG. 20 is a schematic diagram of a reagent card of the immunoassay test device of the present invention;

FIG. 21 is a schematic view of the construction of a kit for an immunoassay test device of the present invention;

FIG. 22 is a second schematic structural view of a cartridge module of the immunoassay detection apparatus of the present invention;

FIG. 23 is an exploded view of a cartridge holder of the cartridge holder module of the immunoassay test device of the present invention;

FIG. 24 is a schematic view of the structure of the outlet of the reagent card of the reagent cartridge of the immunoassay test device of the present invention;

FIG. 25 is a schematic view of the structure of the turntable base of the immunoassay test device of the present invention;

FIG. 26 is an exploded view of a first card pushing mechanism of the immunoassay test device of the present invention;

FIG. 27 is an exploded view of a second card pushing mechanism of the immunoassay test device of the present invention;

FIG. 28 is an exploded view of a third card pushing mechanism of the immunoassay test device of the present invention;

FIG. 29 is a schematic structural view of a stopper mechanism of a third card pushing mechanism of the immunoassay detection apparatus according to the present invention;

FIG. 30 is a top view of the immunoassay test device of the present invention with the cartridge module removed;

FIG. 31 is a first schematic structural diagram of a third card pushing mechanism and a third card pushing mechanism stopper of the immunoassay detection apparatus of the present invention;

FIG. 32 is a second schematic structural view of a third card pushing mechanism and a third block of the card pushing mechanism of the immunoassay detection apparatus of the present invention;

FIG. 33 is a schematic structural view of an X-axis robot assembly of the immunoassay test device of the present invention;

FIG. 34 is a schematic structural view of a Y-axis robot assembly of the immunoassay test device of the present invention;

FIG. 35 is a schematic structural view of a Z-axis robotic arm assembly of the immunoassay test device of the present invention;

FIG. 36 is a schematic view of the structure of the sampling assembly of the immunoassay test device of the present invention;

FIG. 37 is an exploded view of a sampling assembly of the immunoassay test device of the present invention;

FIG. 38 is a second exploded view of the sampling assembly of the immunoassay test device of the present invention;

FIG. 39 is a schematic view of a sampling assembly of the immunoassay test device of the present invention aspirating a TIP head;

FIG. 40 is a schematic external view of an immunoassay test device according to the present invention;

FIG. 41 is a flow chart of collective detection of the immunoassay detection device of the present invention;

FIG. 42 is a flow chart of the emergency test of the immunoassay test device of the present invention.

[ reference numerals ]

1. A base plate; 2. a sample module; 3. a consumable module; 4. a cartridge clip module; 5. an incubation module; 6. a photoelectric data acquisition module; 7. a sampling module; 8. an emergency detection module;

21. a blood collection rack; 22. a diluent rack; 23. a support plate; 24. a blood collection rack guide rail; 25. a diluent rack guide rail; 28. a groove; 29. bumping beads; 30. a bead collision frame; 211. a first sensor;

31. an emergency treatment frame; 32. TIP head disk; 33. uniformly mixing the materials; 34. a TIP header; 35. TIP discard mouth; 36. a blood collection tube; 370. a clamping piece; 38. a TIP head support plate; 39. a graded structure; 40. a bending structure; 301. a gap type structure;

41. a cartridge clip support plate; 42. small magnet; 43. a kit; 431. a small magnet; 432. a lower cover of the kit; 433. the kit is covered; 434. a slot position; 435. a first guide table; 436. a second guide table; 437. a reagent card outlet structure baffle; 44. a cartridge clip baffle; 45. a reagent card; 451. a guide groove; 411. designing a chamfer; 461. the clip baffle plate gap structure; 46. a cartridge channel;

51. an upper heat preservation cover; 52. a lower heat preservation cover; 53. a rotary power module; 54. a second sensor; 55. a turntable; 56. tabletting; 571. connecting the main shaft; 572. expanding and tightening the sleeve; 573. a turntable synchronous large belt wheel; 574. a turntable synchronous small belt wheel; 575. a turntable motor; 576. a turntable synchronous belt; 577. a motor mounting seat;

11. a first card pushing mechanism; 111. a first motor; 112. a first synchronous belt mechanism; 113 small push block one; 114. the first card pushing seat; 115. the first card pushing seat is arranged below the first card pushing seat; 116. a first pinion plate; 117. a first small torsion spring; 118. a first plug screw; 119. a first slider; 12. a second card pushing mechanism; 121. a second motor; 122. a second synchronous belt mechanism; 123. a small push block II; 124. the second card pushing seat; 125. the second card pushing seat is arranged below the first card pushing seat; 126. a second pinion rack; 127. a second small torsion spring; 128. a second plug screw; 129. a second slider; 13. a third card pushing mechanism; 131. a third motor; 132. a third synchronous belt mechanism; 133. a small push block III; 134. the third card pushing seat; 135. the third card pushing seat is arranged below the second card pushing seat; 136. a third pinion rack; 137. a third small torsion spring; 138. thirdly, screwing; 139. a third slider; 48. a top block; 49. a cam block; 483. a fourth spring; 183. a stopper; 184. a pin; 185. sweeping the wharf; 484. an inductor;

61. a light emitting box; 62. tabletting the reagent card; 17. an X-axis robot arm assembly; 171. a fourth motor; 172. a fourth synchronous belt; 173. a first guide rail; 174. a second guide rail; 175. a first timing pulley; 27. a Y-axis robot arm assembly; 271. a fifth motor; 272. a fifth synchronous belt; 273. a third guide rail; 274. a second timing pulley; 37. a Z-axis robotic arm assembly; 371. a sixth motor; 372. a sixth synchronous belt; 373. a fourth guide rail; 374. a third synchronous pulley; 47. a sampling assembly; 178. a syringe assembly;

471. an upper housing shell; 472. a middle housing cover; 473. a lower shell cover; 474. a sleeve; 475. a small spring; 476. a needle head; 477. whether the TIP head has a sensor or not; 478. a connecting shaft; 479. connecting blocks; 480. connecting the small blocks; 481. connecting the small shaft;

100. a housing; 101. a display screen; 102. a hatch door.

As shown, specific structures and devices are labeled in the figures to clearly realize the structures of the embodiments of the present invention, but this is only an illustration and is not intended to limit the present invention to the specific structures, devices and environments, and one of ordinary skill in the art can adjust or modify the devices and environments according to specific needs, and the adjusted or modified devices and environments are still included in the scope of the appended claims.

Detailed Description

The blood sample diluting device and the diluting method for immunoassay testing equipment provided by the invention are described in detail below with reference to the accompanying drawings and specific embodiments. Meanwhile, it is described herein that the following embodiments are the best and preferred embodiments for the purpose of making the embodiments more detailed, and may be implemented in other alternative ways by those skilled in the art; also, the drawings are only for purposes of more particularly describing embodiments and are not intended to limit the invention in any way.

It should be noted that references in the specification to "one embodiment," "an example embodiment," "some embodiments," etc., indicate that the embodiment described may include a particular feature, structure, or characteristic, but every embodiment may not necessarily include the particular feature, structure, or characteristic. Further, when a particular feature, structure, or characteristic is described in connection with an embodiment, it is submitted that it is within the knowledge of one skilled in the relevant art to effect such feature, structure, or characteristic in connection with other embodiments whether or not explicitly described.

In general, terms may be understood at least in part from the context in which they are used. For example, the term "one or more" as used herein may be used to describe any feature, structure, or characteristic in the singular or may be used to describe a combination of features, structures, or characteristics in the plural, depending, at least in part, on the context. Additionally, the term "based on" may be understood as not necessarily intended to convey an exclusive set of factors, but may instead allow for the presence of other factors not necessarily explicitly described, depending at least in part on the context.

As used herein, the term "nominal" refers to a desired or target value, and a range above and/or below the desired value, of a characteristic or parameter set during a design phase of a production or manufacturing process for a component or process operation. The range of values may be due to slight variations in manufacturing processes or tolerances. As used herein, the term "about" indicates a value of a given amount that may vary based on the particular technology node associated with the subject semiconductor device. The term "about" may indicate a given amount of a value that varies, for example, within 5% -15% of the value (e.g., ± 5%, ± 10% or ± 15% of the value), based on the particular technology node.

It is understood that the meaning of "on … …," "over … …," and "over … …" in this disclosure should be read in the broadest manner, such that "on … …" means not only "directly on" but also includes the meaning of "on" something with intervening features or layers therebetween, and "on … …" or "over … …" means not only "on" or "over" something, but may also include the meaning of "on" or "over" without intervening features or layers therebetween.

Furthermore, spatially relative terms such as "below …," "below …," "lower," "above …," "upper," and the like may be used herein for ease of description to describe one element or feature's relationship to another element or feature or elements, as illustrated in the figures. Spatially relative terms are intended to encompass different orientations in use or operation of the device in addition to the orientation depicted in the figures. The device may be otherwise oriented and the spatially relative descriptors used herein interpreted accordingly.

As shown in fig. 1, an embodiment of the present invention provides an immunoassay detection apparatus, which includes a bottom plate 1, wherein the bottom plate 1 is provided with a sample module 2, a consumable module 3, a cartridge clip module 4, an incubation module 5, a photoelectric data acquisition module 6, a sampling module 7, and an emergency detection module 8.

As shown in fig. 2, 8-11, the sample module 2 includes a support plate 23, the support plate 23 is mounted on the base plate 1, and the blood collection racks 21 and the diluent racks 22 are mounted on the support plate 23, preferably four rows of the blood collection racks 21 and one row of the diluent racks 22 are mounted. Five corresponding guide rail grooves are distributed on the support plate 23, namely four blood collecting rack guide rails 24 and one diluent rack guide rail 25, and the blood collecting rack 21 and the diluent rack 22 are respectively arranged on the blood collecting rack guide rails 24 and the diluent rack guide rails 25 in a drawing manner. Specifically, the insertion part of the guide rail groove is provided with a notch structure to form a size gradually changing form, the size is gradually reduced from large to small, the guide rail groove has a guiding function, correspondingly, the bottoms of the blood sampling frame 21 and the diluent frame 22 are respectively provided with a guide block, the guide blocks can be inserted into the guide rail groove, the shape of the guide blocks is matched with the shape of the guide rail groove, and the insertion part of the guide blocks is provided with a notch structure to form a size gradually changing form, the size is gradually reduced from small to large, the blood sampling frame 21 and the diluent frame 22 can be smoothly drawn and inserted, and the blood sampling frame 21 can be conveniently inserted into the corresponding guide rail groove; there is the backplate at the back of backup pad 23 through the screw installation, install the curb plate respectively through the screw about, be equipped with on the backplate and bump pearl frame 30, the tail end of blood sampling frame 21 and diluent frame 22 is equipped with recess 28, recess 28 with bump pearl 29 looks gomphosis on bumping pearl frame 30, realize the location of diluent frame 22 and backplate, the location of blood sampling frame 21 and backplate, it is fixed to bump the pearl and promoted the convenience and promoted compact structure nature with the design of guide rail extraction, the human-computer interaction problem that doctor and machine placed at the sample has been solved in this design, the experience that the instrument used is felt has been promoted. A first sensor 211 is arranged on a rear baffle plate at the position of the blood sampling frame 21, and after the first sensor 211 detects that the blood sampling frame 21 is inserted into the guide rail groove, a code scanner arranged on the bottom plate 1 automatically records one-dimensional code information on the blood sampling frame 21, so that the detection sample position and the patient information of the blood sampling frame 21 are judged.

The blood sampling frame 21 and the diluent frame 22 are both provided with a push handle, and the bottom of the push handle is provided with an inward concave type curved surface structure, so that the blood sampling frame and the diluent frame are convenient to take; be equipped with heparin tube installation unit on the blood sampling frame 21, heparin tube installation unit is open type structure, easy to assemble heparin tube 36 to, can follow the one-dimensional sign indicating number information of its opening part discernment heparin tube 36.

As shown in fig. 2, 12-16, the consumable module 3 comprises a consumable body, a TIP head support plate 38 and a blending plate 33 are arranged on the top of the consumable body, an emergency stand 31 and a TIP discarding device are arranged on the side surface of the consumable body, and a TIP head plate 32 is fixed on the TIP head support plate 38 through a clamping piece 370; the emergency treatment frame 31 is arranged close to the side wall of the sample module 2, the upper part of the emergency treatment frame 31 adopts an opening structure 301 and reducing treatment, and the guiding function is played for placing the blood collection tube 36 to the emergency treatment frame 31; a TIP discarding device is arranged beside the emergency frame 31, a TIP discarding nozzle 35 is arranged on the TIP discarding device, the TIP discarding nozzle 35 adopts a gradual change type structure 39, and the TIP head 34 is convenient to replace as the TIP head gradually changes from small to large from the TIP head insertion position to the TIP nozzle discarding port; the clamping piece 370 adopts a two-section bending structure 40, one clamping piece 370 is respectively arranged on each of two sides of the TIP head disk 32, the two sides simultaneously clamp the TIP head supporting plate 38 inwards to finish the fixation of the TIP head supporting plate 38 and the TIP head disk 32, and the clamping effect can be realized when the TIP head disk 32 is replaced; the TIP head disk 32 is provided with a plurality of TIP head 34 placing positions, the mixing disk 33 is arranged beside the TIP head disk 32, the sampling module 7 can replace the TIP head 34 through the TIP discarding nozzle 35, and the sampling module 7 can sequentially add samples in the blood sampling rack 21 and diluent in the diluent rack 22 into the mixing disk 33 to be mixed uniformly. TIP abandons mouth 35 and has adopted the design of gradual change formula opening to promote the efficiency that the consumptive material was changed, and emergency call frame 31 adopts the design of opening formula structure 301 to promote the convenience that the instrument dealt with the emergency call condition, and the bending structure 40 of clamping piece 370 has promoted the overall stability of TIP head dish 32 on consumptive material module 3. A waste bin is positioned below the TIP discard nozzle 35, and the used TIP head 34 falls directly from the TIP discard nozzle 35 into the waste bin below.

As shown in fig. 3, 17-24, a cartridge clip module 4 is disposed right above the card pushing mechanism of the bottom plate 1, the cartridge clip module 4 includes a cartridge clip support plate 41, a plurality of cartridge clip baffles 44 are disposed on the cartridge clip support plate 41, a corresponding reagent box channel 46 is formed between two cartridge clip baffles 44, and a plurality of reagent boxes 43 vertically disposed are inserted into the corresponding reagent box channel 46. Be equipped with cartridge clip baffle opening structure 461 on the cartridge clip baffle 44, during every kit 43 inserted cartridge clip baffle 44 from cartridge clip baffle opening structure 461, be equipped with two little magnet stones 42 from top to bottom between two cartridge clip baffles 44, two upper and lower little magnet 431 on little magnet stone 42 and the kit 43 attract each other to accomplish fixedly, the guide problem of kit 43 has been solved in this kind of design, the convenience of changing kit 43 has been promoted and the accuracy difficult problem of having solved kit 43 location has promoted the convenience and the stability of the change kit 43 of instrument.

As shown in fig. 17-24, the reagent kit 43 includes a reagent kit upper cover 433 and a reagent kit lower cover 432, a slot 434 for placing the small magnet 431 is formed in the reagent kit lower cover 432, the small magnet 431 is placed in the slot 434, a first guide table 435 is formed in the reagent kit lower cover 432, a second guide table 436 is formed in the reagent kit upper cover 433, and the two guide tables are matched with each other; the first guide table 435 is a convex rib and is arranged on the side surface of the lower kit cover 432, and the middle of the second guide table 436 is provided with a convex rib which is arranged in the middle of the inner part of the upper kit cover 433; the reagent card 45 is arranged in the reagent box 43, the front side and the side edge of the reagent card 45 are respectively provided with a guide groove 451, the guide groove 451 at the front side is matched with a convex rib in the middle of the second guide table 436, and the guide groove 451 at the side edge is matched with the first guide table 435, so that the function of sliding the reagent card 45 in the reagent box 43 from top to bottom is realized, and the side turning can be prevented; the reagent box lower cover 432 is provided with a groove position 434, a buckle is arranged at a position corresponding to the reagent box upper cover 433, and the reagent box lower cover 432 and the reagent box upper cover 433 are buckled together through the groove position 434 and the buckle to combine the complete reagent box 43. A reagent card outlet structure is arranged at one corner opposite to the first 435 guide table at the lower end of the reagent kit 43, the reagent card outlet structure comprises a torsion spring and a reagent card outlet structure blocking piece 437 connected with the torsion spring, a gap is arranged at the lower end of the reagent card 45, the first card pushing mechanism 11 pushes the reagent card 45 at the gap, the reagent card 45 pushes against the reagent card outlet structure blocking piece 437, and the torsion spring drives the reagent card outlet structure blocking piece 437 to rotate and move away so that the reagent card 45 is pushed into the rotary table 55 by the first card pushing mechanism 11.

As shown in fig. 22, the clip retainer 44 and the clip support plate 41 are respectively provided with a chamfer 411, which can guide the reagent kit 43 and facilitate smooth insertion.

As shown in fig. 4, 25-29, the incubation module 5 includes an upper heat-preservation cover 51, a lower heat-preservation cover 52, a rotary power module 53 and a second sensor 54, the second sensor 54 is disposed on the upper heat-preservation cover 51, the rotary power module 53 is disposed below the heat-preservation cover, and includes a turntable 55 and a turntable motor 575; the second sensor 54 is used to detect the absence of the reagent card 45 at the current location, and to prevent the absence of the reagent card 45 when the blended sample is added.

A card pushing mechanism is arranged on the corresponding bottom plate 1 below the incubation module 5, and is respectively a first card pushing mechanism 11, a second card pushing mechanism 12 and a third card pushing mechanism 13; a pressing sheet 56 is arranged on the turntable 55, a connecting main shaft 571 is connected below the turntable 55, a bearing sleeve is connected below the bottom plate 1, the connecting main shaft 571 is fixed on a bearing, a bearing inner ring is installed and connected with the main shaft 571, and a bearing outer ring is installed in the bearing sleeve; a large turntable synchronous belt pulley 573 and an expansion sleeve 572 are sequentially mounted on the connecting main shaft 571, a motor mounting seat 577 is mounted on the base plate 1, a turntable motor 575 is mounted on the motor mounting seat 577, a small turntable synchronous belt pulley 574 is mounted on an output shaft of the turntable motor 575, and a turntable synchronous belt 576 is mounted on the large turntable synchronous belt pulley 573 and the small turntable synchronous belt pulley 574. The turntable motor 575 drives the turntable 55 to rotate through the turntable synchronous belt 576.

As shown in fig. 1, 4, 26 and 30, the bottom plate 1 is provided with 4 first card pushing mechanisms 11 corresponding to the reagent kit channels 46, the reagent kit channels 46 are provided with 4 first card pushing mechanisms 11 corresponding to the reagent kit channels 46, the top of the bottom plate 1 is provided with the first synchronous belt mechanism 112, the bottom of the bottom plate 1 is provided with the first motor 111 capable of driving the first synchronous belt mechanism 112, the synchronous belt of the first synchronous belt mechanism 112 is provided with the first card pushing mechanisms 11, specifically, the first slide block 119 is mounted on the synchronous belt, and the first slide block 119 can move along with the synchronous belt.

The first card pushing mechanism 11 comprises a first small pushing block 113, a first card pushing seat upper 114, a first card pushing seat lower 115, a first pinion plate 116, a first small torsion spring 117 and a first plug screw 118, wherein the first small torsion spring 117 is sleeved on the first plug screw 118, the first small pushing block 113 is installed on the first card pushing seat upper 114 through the first plug screw 118 and the first small torsion spring 117, the first card pushing seat lower 115 is installed on the first sliding block 119, the first card pushing seat upper 114 is installed above the first card pushing seat lower 115, the first pinion plate 116 is installed on the first card pushing seat lower 115, and the first synchronous belt mechanism 112 drives the first sliding block 119 to perform reciprocating card pushing motion so as to drive the first small pushing block first 113 to perform reciprocating motion.

As shown in fig. 1, 4, 27 and 30, a second card pushing mechanism 12 is disposed on the bottom plate 1 at a side of the first card pushing mechanism 11, a second timing belt mechanism 122 is mounted on the top of the bottom plate 1, a second motor 121 capable of driving the second timing belt mechanism 122 is mounted on the bottom of the bottom plate 1, the second card pushing mechanism 12 is mounted on a timing belt of the second timing belt mechanism 122, specifically, a second slider 129 is mounted on the timing belt, and the second slider 129 can move along with the timing belt.

The second card pushing mechanism 12 includes a second small pushing block 123, a second card pushing seat upper 124, a second card pushing seat lower 125, a second pinion 126, a second small torsion spring 127, a second stopper screw 128, the second small torsion spring 127 is sleeved on the second stopper screw 128, the second small pushing block 123 is installed on the second card pushing seat upper 124 through the second stopper screw 128 and the second small torsion spring 127, the second card pushing seat lower 125 is installed on the second slider 129, the second card pushing seat upper 124 is installed above the second card pushing seat lower 125, the second pinion 126 is installed on the second card pushing seat lower 125, the second synchronous belt mechanism 122 drives the second slider 129 to perform reciprocating card pushing movement, and further drives the second small pushing block 123 to perform reciprocating movement.

As shown in fig. 1, 4, and 28 to 32, a third card pushing mechanism 13 is disposed on the bottom plate 1 near the sample module 2, a third timing belt mechanism 132 is mounted on the top of the bottom plate 1, a third motor 131 capable of driving the third timing belt mechanism 132 is mounted on the bottom of the bottom plate 1, the third card pushing mechanism 13 is mounted on the timing belt of the third timing belt mechanism 132, specifically, a third slider 139 is mounted on the timing belt, and the third slider 139 is capable of following the timing belt.

The third card pushing mechanism 13 includes a small pushing block three 133, a third card pushing seat upper 134, a third card pushing seat lower 135, a third pinion plate 136, a third small torsion spring 137, a third stopper screw 138, a top block 48 and a cam block 49, the third small torsion spring 137 is sleeved on the third stopper screw 138, the small pushing block three 133 is installed on the third card pushing seat upper 134 through the third stopper screw 138 and the third small torsion spring 137, the cam block 49 is installed on the third card pushing seat upper 134, the third card pushing seat lower 135 is installed on the third slider 139, the third card pushing seat upper 134 is installed above the third card pushing seat lower 135, the third pinion plate 136 is installed on the third card pushing seat lower 135, and the third synchronous belt mechanism 132 drives the small pushing block three 133 to perform reciprocating card pushing motion.

As shown in fig. 30, the reagent cards 45 in the reagent cartridges 43 are pushed into the rotary table 55 from the card pushing paths by the first card pushing mechanism 11, the pressing sheet 56 presses the reagent cards 45 into the rotary table 55, the rotary table 55 rotates to the card ejecting path located at the outermost side of the apparatus beside the card pushing paths, and the second card pushing mechanism 12 pushes the reagent cards 45 out of the rotary table 55.

As shown in fig. 30 to 32, when an emergency treatment is required, the reagent card 45 is inserted into an emergency treatment channel beside the consumable module 3, the emergency treatment channel is provided with a top block 48, the top block 48 comprises a fourth spring 483, one end of the fourth spring 483 is mounted on the clip support plate 41, the other end of the fourth spring 483 is connected with a stop block 183, the bottom end of the stop block 183 is mounted with an L-shaped frame, and a pin 184 is mounted on the L-shaped frame. The stopper 183 stops the reagent card 45 at the initial position of the ejector block 48, the sensor 484 arranged on the clip support plate 41 senses the insertion of the reagent card 45, the third motor 131 starts to drive the third synchronous belt mechanism 132 to move, the cam block 49 on the third card pushing seat 134 on the third card pushing mechanism 13 moves along with the third slider 139 of the third card pushing mechanism 13 to push the pin 184 on the ejector block 48, the fourth spring 483 is pressed, the pin 184 is pushed to drive the stopper 183 to move open, so that the reagent card 45 enters the card pushing channel of the third card pushing mechanism 13 from the channel of the emergency detection module 8, the third synchronous belt mechanism 132 continues to move, the small ejector block three 133 of the third card pushing mechanism 13 pushes the reagent card 45 into the rotary table 55 under the driving force, the ejector block 183 moves under the rebounding force of the fourth spring 483, the ejector block 48 returns to the initial position, the rotary power module 53 drives the rotary table 55 to rotate, the turntable 55 rotates to the card eject path, and the second card pushing mechanism 12 pushes the reagent card 45 out of the turntable 55. The second card pushing mechanism 12 pushes the incubated reagent card 45 into the waste card box.

As shown in fig. 5, the cartridge clip support plate 41 is provided with the photoelectric data acquisition module 6, the photoelectric data acquisition module 6 includes a light emitting box 61 and a reagent card pressing plate 62, before the second card pushing mechanism 12 pushes the card to the waste card box, the reagent card 45 passes through the photoelectric data acquisition module 6, the reagent card pressing plate 62 presses the reagent card 45, and the light emitting box 61 scans and acquires data on the reagent card 45.

As shown in fig. 6, 33-39, a sampling module 7 is provided on the back of the consumable module 3 on the bottom plate 1. The sampling module 7 comprises an X-axis mechanical arm assembly 17, a Y-axis mechanical arm assembly 27, a Z-axis mechanical arm assembly 37 and a sampling assembly 47, the sampling module 7 can displace in a three-axis space, and the sampling assembly 47 of the sampling module 7 can suck and spit liquid. The X-axis robot arm assembly 17 includes a first frame, a fourth motor 171 is installed at the bottom of the first frame, two first synchronous pulleys 175 are installed at the top of the first frame, the fourth motor 171 drives one of the first synchronous pulleys 175 to rotate, fourth synchronous belts 172 are installed on the two first synchronous pulleys 175, a first synchronous slider is installed on the fourth synchronous belt 172, a first guide rail 173 and a second guide rail 174 are installed at the top of the first frame, fourth sliders are installed on the first guide rail 173 and the second guide rail 174, and the fourth motor 171 drives the first synchronous pulley 175 to move, so that the robot arm can move on the first guide rail 173 and the second guide rail 174. The X-axis mechanical arm assembly 17 adopts a mode of double guide rails and synchronous belts, so that the mechanical arm can run more stably in the X-axis direction.

The Y-axis mechanical arm assembly 27 comprises a second frame, the second frame is mounted on the first synchronous sliding block and the fourth sliding block, the fourth motor 171 is driven to drive the first synchronous belt pulley 175 to rotate, the fourth synchronous belt 172 moves while driving the second frame to move through the first synchronous sliding block, and the movement of the Y-axis mechanical arm assembly 27 in the X direction is realized.

The Y-axis robot assembly 27 further includes two second timing pulleys 274 disposed on the front side surface of the second frame, and a fifth motor 271 disposed on the rear side surface, the fifth motor 271 drives one of the second timing pulleys 274, a fifth timing belt 272 is mounted on the two second timing pulleys 274, a third guide 273 is disposed on the front side surface of the second frame, and second timing sliders are mounted on the fifth timing belt 272 and the third guide 273.

The Z-axis mechanical arm assembly 37 includes a third frame, the third frame is mounted on the second synchronous slide block, the fifth motor 271 drives the second synchronous belt wheel 274 to rotate, and the fifth synchronous belt 272 drives the third frame to move by driving the second synchronous slide block while moving, so as to realize the movement of the Z-axis mechanical arm assembly 37 in the Y direction.

The Z-axis robot assembly 37 further includes two third timing pulleys 374 disposed on the front side surface of the third frame, and a sixth motor 371 disposed on the rear side surface, wherein the sixth motor 371 drives one of the third timing pulleys 374, sixth timing belts 372 are mounted on the two third timing pulleys 374, a fourth guide rail 373 is disposed on the front side surface of the third frame, and third timing sliders are mounted on the sixth timing belts 372 and the fourth guide rail 373.

Sampling component 47 installs on third synchronous slide, and sixth hold-in range 372 drives third synchronous pulley 374 and rotates, and the removal of sixth hold-in range 372 drives sampling component 47 through driving third synchronous slide simultaneously, realizes sampling component 47 in the removal of Z direction, specifically, sampling component 47 passes through the connecting piece with third synchronous slide and is connected. The lower end of an X-axis mechanical arm assembly 17 of the sampling module 7 is provided with an injector assembly 178, the injector assembly 178 can be used for sucking a sample, and the Y, Z mechanical arm assembly adopts a transmission mode of a guide rail and a synchronous belt to enable the mechanical arm to run more efficiently and stably on a Z axis.

36-39, the sampling assembly 47 includes an upper housing 471, a middle housing 472, a lower housing 473, a sleeve 474, a small spring 475, a needle 476, a TIP head presence sensor 477, a connecting shaft 478, a connecting block 479, a connecting block 480, a connecting stub 481, and a TIP head 34. The connecting shaft 478 is connected with the connecting block 479, the connecting small block 480 is fixed on the connecting block 479, the TIP head sensor 477 is fixed on the connecting small block 480, the connecting small shaft 481 is connected with the connecting block 479, the small spring 475 is sleeved on the connecting small shaft 481, the sleeve 474 is connected with the connecting small shaft 481, the lower shell cover 473 and the middle shell cover 472 are assembled with the upper shell cover 471 from bottom to top and cover other components except the sleeve 474, the needle 476 and the connecting small shaft 481, and the needle 476 is assembled with the connecting small shaft 481.

The sampling module 47 is plugged into the TIP 34, the further TIP presence sensor 477 detects that the TIP 34 has been taken, the sampling module 47 goes to the sample module 2 to suck up the sample and diluent, move it to the mixing tray 33, mix it and add it to the reagent card 45 to be incubated. During the picking of the TIP 34 by the sampling assembly 47, the sampling assembly 47 is moved downward and the needle 476 is inserted into the TIP 34, whereupon the small spring 475 of the sampling assembly 47 contracts and the TIP 34 pick-up is complete and the TIP presence sensor 477 detects the TIP 34; the sampling assembly 47 moves upward, at which time the small spring 475 of the sampling assembly 47 resets, causing the needle 476 to reset. After the uniform mixing is completed, in the process of discarding the TIP head 34, the sampling assembly 47 moves downwards, the TIP head 34 moves into the TIP discarding nozzle 35, at this time, the TIP head 34 is located at the large caliber position of the TIP discarding nozzle 35, the TIP head 34 moves into the TIP discarding nozzle 35 to the small caliber position of the TIP discarding nozzle 35, in the process of moving upwards the sampling assembly 47, the TIP head 34 is clamped in the small caliber position of the TIP discarding nozzle 35, and the TIP head 34 discarding is completed. The needle 476 is then moved upwardly to discard the TIP 34 through the TIP disposal nozzle 35, completing the replacement of the TIP 34, the TIP presence sensor 477 detects that the TIP 34 has been disposed of, the spring 475 resets the sleeve 474, and the sampling assembly 47 can repeatedly draw and drain fluid.

Upper casing cover 471, well casing cover 472, lower casing cover 473 adopt disconnect-type structural design, and the limit function to the sleeve is upper and lower has been realized in the time of easy to assemble, and sleeve 474's structural design, the more convenient realization detect the TIP head have or not function, and this structural design's advantage lies in that it has realized the function of sample subassembly 47 with clearer structure.

As shown in fig. 7 and fig. 32, an emergency detection module 8 is disposed beside the clip module 4 on the base plate 1, the reagent card 45 can be directly inserted from the emergency detection module 8, the scanning head 185 scans information of the reagent card 45, the sensor 484 disposed on the clip support plate 41 senses insertion of the reagent card 45, the third motor 131 starts to drive the third synchronous belt mechanism 132 to move, the cam block 49 on the third card pushing seat 134 on the third card pushing mechanism 13 moves along with the third slider 139 of the third card pushing mechanism 13, the pin 184 on the pushing block 48 and the fourth spring 483 are pressed, the pin 184 is pushed to drive the stopper 183 to move open, so that the reagent card 45 enters the card pushing channel of the third card pushing mechanism 13 from the channel of the emergency detection module 8, the third synchronous belt mechanism 132 continues to rotate, the small pushing block 133 of the third card pushing mechanism 13 drives the reagent card 45 to push into the rotary table 55, under the action of the resilience of the fourth spring 483, promote dog 183 and remove, kicking block 48 returns to initial position, and rotatory power module 53 drives the carousel 55 rotatory, and carousel 55 is rotatory to the card withdrawal passageway after the incubation time, and second pushes away card mechanism 12 and pushes out carousel 55 with reagent card 45, and after photoelectric data collection module 6 data collection, second pushes away card mechanism 12 and pushes away the reagent card 45 after the completion of hatching to useless card box.

As shown in fig. 40, the exterior of the present invention is provided with a housing 100, the housing 100 is provided with a hatch 102, the hatch 102 is provided outside the sample module 2 and the consumable module 3, and the sample or TIP head disk 32 and the mixing disk 33 can be replaced by opening the hatch 102. The hatch door 102 top is equipped with display screen 101, display screen 101 and the first sensor 211 on the side shield behind blood sampling frame 21, photoelectric data acquisition module 6 electric connection, the one-dimensional code of detector scanning and the information that photoelectric data acquisition module 6 gathered can spread into the control system in display screen 101 in real time, can show sample information, position, kind, incubation time, the incubation category that can control on the display screen 101 interface, can divide into groups kind to incubate.

As shown in fig. 41, an immunoassay detection method, the method comprising the steps of:

s1, the guide blocks of the blood sampling frame 21 and the diluent frame 22 in the sample module 2 are inserted into the guide rail grooves on the support plate 23 in a sliding manner, and when the insertion of the blood sampling frame 21 is sensed by the first sensor 211 arranged on the rear baffle plate of the position of the blood sampling frame 21, the one-dimensional code information on the blood sampling frame 21 is automatically recorded by the code scanner arranged on the bottom plate 1 so as to judge the detection sample position and the patient information of the blood sampling frame 21.

S2, the reagent card 45 is loaded into the reagent cartridge 43 by aligning the side guide groove 451 of the reagent card 45 with the protruding ribs of the two guide stages, and the reagent cartridge 43 is inserted into the reagent cartridge passage 46 from the clip retainer slit structure 461 on the clip retainer 44 of the clip module 4.

S3, after the information of the detection items is recorded, the fourth motor 171 of the X-axis mechanical arm assembly 17 drives the first synchronous pulley 175 to move to drive the fourth slider and the first synchronous slider on the X-axis mechanical arm assembly 17 to move on the first guide rail 173 and the second guide rail 174, namely, the movement of the Y-axis mechanical arm assembly 27 on the X axis is realized, the fifth motor 271 of the Y-axis mechanical arm assembly 27 drives the second synchronous pulley 274 to move to drive the second synchronous slider on the Y-axis mechanical arm assembly 27 to move on the third guide rail 273, namely, the movement of the Z-axis mechanical arm assembly 37 on the Y axis is realized, the sixth motor 371 of the Z-axis mechanical arm assembly 37 drives the third synchronous pulley 374 to move to drive the third synchronous slider on the Z-axis mechanical arm assembly 37 to move on the fourth guide rail 373, namely, the up-and-down movement of the sampling assembly 47 on the Z axis is realized, the mechanical arm assembly moves in three-dimensional space to drive the sampling assembly 47 to move to the TIP head disk 32 at the preset position, the sampling assembly 47 is moved downward and the needle 476 is inserted into the TIP head 34, at which point the small spring 475 of the sampling assembly 47 contracts and the TIP head 34 pickup is complete and the TIP head presence sensor 477 detects the TIP head 34; the sampling component 47 moves upwards, at this time, the small spring 475 of the sampling component 47 resets to drive the needle 476 to reset, the mechanical arm component drives the sampling component 47 to move to the position above the blood sampling frame 21 of the sample module 2, and the sixth motor 371 drives the third synchronous belt pulley 374 to move, so that the sampling component 47 moves downwards on the fourth guide rail 373 to suck a sample, and then moves to the position above the diluent frame 22 to suck diluent in the same action. The mechanical arm assembly drives the sampling assembly 47 to move above the blending tray 33 in the XY direction, and the Z-axis mechanical arm assembly 37 drives the sampling assembly 47 to move downwards, so that the sample and the diluent are blended on the blending tray 33.

S4, the reagent card 45 in the reagent cartridge 43 in the cartridge clip module 4 slides downward along the guide table in the reagent cartridge 43 and enters the card pushing channel from the reagent card outlet structure, the first motor 111 of the first card pushing mechanism 11 drives the first synchronous belt mechanism 112 to drive the first slide block 119 to perform reciprocating card pushing motion, so as to drive the first small push block 113 to perform reciprocating motion, and the first small push block 113 hooks the notch below the reagent card 45 to push the reagent card 45 to enter the turntable 55; at this time, the mechanical arm assembly of the sampling module 7 drives the sampling assembly 47 to suck the mixed sample, and the mixed sample moves to the reagent card 45 in the turntable 55 according to a predetermined direction, and the mixed sample is added into the reagent card 45 for reaction.

S5, after the uniform mixing is completed, the mechanical arm component drives the sampling component 47 to move to the upper side of the discarding device of the consumable module 3 in the XY axis direction, the sampling component 47 moves downwards, the TIP head 34 moves into the TIP discarding nozzle 35, at the moment, the TIP head 34 is located at the large caliber of the TIP discarding nozzle 35, the TIP head 34 moves into the small caliber of the TIP discarding nozzle 35 in the TIP discarding nozzle 35, and in the upward moving process of the sampling component 47, the TIP head 34 is clamped in the small caliber of the TIP discarding nozzle 35, and the TIP head 34 discarding is completed. The needle 476 is then moved upwardly to discard the TIP head 34 through the TIP disposal nozzle 35 to complete replacement of the TIP head 34, the TIP head presence sensor 477 detects that the TIP head 34 has been disposed of, the spring 475 resets the sleeve 474, and the sampling assembly 47 can repeatedly draw in and drain fluid.

S6, after the reaction is completed, the rotating disc 55 rotates to the card withdrawing channel, the second motor 121 in the second card pushing mechanism 12 installed on the base plate 1 drives the second synchronous belt mechanism 122 to move to drive the second sliding block 129 to perform reciprocating card pushing movement, and further drives the second small pushing block 123 to perform reciprocating movement, the second small pushing block 123 hooks the gap below the reagent card 45 to push the reagent card 45 into the photoelectric data acquisition module 6, the reagent card pressing piece 62 of the photoelectric data acquisition module 6 on the elastic clamp supporting plate 41 presses the reagent card 45, the light emitting box 61 performs data acquisition and completes detection, after the detection is completed, the second motor 121 in the second card pushing mechanism 12 installed on the base plate 1 drives the second synchronous belt mechanism 122 to move to drive the second sliding block 129 to perform reciprocating card pushing movement, and further drives the second small pushing block 123 to perform reciprocating movement, the second small pushing block 123 hooks the gap below the reagent card 45 to push the reagent card 45 out, from the carousel 55 to the discard cassette.

As shown in fig. 42, an emergency condition;

s11, placing the blood collection tube 36 into the emergency stand 31, placing the sampling assembly 47 into the blending tray 33 to blend the emergency sample and the diluent, and inserting the reagent card 45 into the channel of the emergency detection module 8.

S22, when the sensor 484 on the clip support plate 41 senses the insertion of the reagent card 45, the third motor 131 starts to drive the third timing belt 132 to move, the cam block 49 on the third card pushing seat 134 on the third card pushing mechanism 13 moves along with the third slide block 139 of the third card pushing mechanism 13, the pin 184 on the ejector block 48 is pushed, the fourth spring 483 is pressed, the pin 184 is pushed to drive the stopper 183 to move open, so that the reagent card 45 enters the card pushing channel of the third card pushing mechanism 13 from the channel of the emergency detection module 8, the third synchronous belt mechanism 132 continues to rotate, the small third pushing block 133 of the third card pushing mechanism 13 pushes the reagent card 45 into the rotary disc 55 under the driving of the small third pushing block for incubation, under the resilience of the fourth spring 483, the push block 183 moves, the top block 48 returns to the initial position, the rotary power module 53 drives the rotary disc 55 to rotate, the sampling module 7 adds the mixed sample of the emergency treatment into the reagent card 45 for reaction.

And S33, after the incubation time is over, the rotating disc 55 rotates to the card withdrawing channel, the second card pushing mechanism 12 pushes the reagent card 45 into the photoelectric data acquisition module 6, and after the photoelectric data acquisition module 6 acquires data and completes detection, the second card pushing mechanism 12 pushes the incubated reagent card 45 into a waste card box.

The blood sample diluting device and the blood sample diluting method for the immunoassay detection equipment have the advantages that the sample module in the blood sample diluting device and the blood sample diluting method for the immunoassay detection equipment adopts a guide rail drawing design, so that the problem of human-computer interaction between a doctor and a machine in sample placement can be solved, the experience and the convenience of using an instrument are improved, the drawing of the blood sampling frame is more convenient due to the design of the guide rail, the convenience of the instrument for emergency situations is improved due to the addition of the emergency treatment frame to the consumable module, and the discarding efficiency is improved due to the adoption of the gradual-change gap design of the TIP discarding nozzle.

The invention changes the connection mode of the transmission spline shaft of the mechanical arm, thereby realizing the sample adding function of the mechanical arm of the analytical instrument in a lower-cost and more stable motion mode of the guide rail and the synchronous belt; the sampling assembly is provided with the TIP existence device, so that the existence of the TIP of the sampling device can be detected more efficiently and automatically, the trouble of manual replacement is avoided, and the TIP head of the sampling assembly can be automatically separated from the sampling assembly, so that the discarding efficiency is improved.

The invention is intended to cover alternatives, modifications, equivalents, and alternatives that may be included within the spirit and scope of the invention. In the following description of the preferred embodiments of the present invention, specific details are set forth in order to provide a thorough understanding of the present invention, and it will be apparent to those skilled in the art that the present invention may be practiced without these specific details. In other instances, well-known methods, procedures, components, and circuits have not been described in detail as not to unnecessarily obscure aspects of the present invention.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims

1. A blood sample diluting device for immunoassay detection equipment is characterized by comprising a sampling module, a consumable module and a sample module, wherein the sampling module comprises a sampling assembly, an X-axis mechanical arm assembly, a Y-axis mechanical arm assembly and a Z-axis mechanical arm assembly which are used for driving the sampling assembly X, Y, Z to move in directions;

the sampling assembly comprises a needle head which can pick up or discard the TIP head and a TIP head sensor which can detect whether the TIP head is arranged on the needle head or not;

the sampling assembly comprises a connecting shaft, the connecting shaft is connected with a connecting block, a connecting small block is fixed on the connecting block, a TIP head sensor is fixed on the connecting small block, the connecting small shaft is connected with the connecting block, a small spring is sleeved on the connecting small shaft, a sleeve is connected with the connecting small shaft, a lower shell cover and a middle shell cover are arranged with an upper shell cover from bottom to top and cover other components except the sleeve, a needle head and the connecting small shaft, and the needle head is arranged with the connecting small shaft;

the TIP discarding nozzle with the gradually-changing structure comprises a large-diameter part and a small-diameter part, and the large-diameter part and the small-diameter part are in transition communication.

2. The blood sample diluting device for immunoassay testing equipment according to claim 1, wherein the X-axis mechanical arm assembly comprises a first frame, a fourth motor is installed at the bottom of the first frame, two first synchronous pulleys are installed at the top of the first frame, the fourth motor drives one of the first synchronous pulleys to rotate, a fourth synchronous belt is installed on the two first synchronous pulleys, a first synchronous slider is installed on the fourth synchronous belt, a first guide rail and a second guide rail are arranged at the top of the first frame, a fourth slider is installed on the first guide rail and the second guide rail, and the Y-axis mechanical arm assembly is installed on the fourth slider and the first synchronous slider.

3. The blood sample dilution device for an immunoassay test apparatus according to claim 1, wherein the Y-axis robotic arm assembly includes a second frame mounted on the first and fourth synchronized slides; the Z-axis mechanical arm assembly is characterized in that two second synchronous belt wheels are arranged on the front side face of the second rack, a fifth motor is arranged on the rear side face of the second rack, the fifth motor drives one of the second synchronous belt wheels, fifth synchronous belts are installed on the two second synchronous belt wheels, a third guide rail is arranged on the front side face of the second rack, second synchronous sliding blocks are installed on the fifth synchronous belts and the third guide rail, and the Z-axis mechanical arm assembly is installed on the second synchronous sliding blocks.

4. The blood sample dilution device for an immunoassay test apparatus according to claim 1, wherein the Z-axis robotic arm assembly includes a third frame mounted on a second synchronized slide; be provided with the sixth motor on two third synchronous pulleys, the trailing flank on the leading flank of third frame, sixth motor drive one of them third synchronous pulley is two install the sixth hold-in range on the third synchronous pulley be provided with the fourth guide rail on the leading flank of third frame the sixth hold-in range with install the third synchronous slide block on the fourth guide rail install on the third synchronous slide block the sampling subassembly.

5. The blood sample diluting device for the immunoassay detection apparatus according to claim 1, wherein a TIP head support plate and a mixing disk are provided on the top of the consumable body, and a TIP head disk is provided above the TIP head support plate.

6. The blood sample diluting device for immunoassay testing apparatus as set forth in claim 5, wherein a clamping piece is disposed on each side of said TIP head disk, said clamping piece having a two-segment bent structure, and both sides simultaneously clamping said TIP head support plate inward to complete the fixation of said TIP head support plate and said TIP head disk.

7. The blood sample diluting device for immunoassay testing equipment according to claim 1, wherein the blood sampling frame and the diluent frame are both provided with a pushing handle, the bottom of the pushing handle is provided with an inwardly concave curved surface structure, the blood sampling frame is provided with a blood sampling tube mounting unit, and the blood sampling tube mounting unit is of an open structure.

8. A blood sample diluting method for an immunoassay test device, comprising the blood sample diluting device for an immunoassay test device according to any one of claims 1 to 7, wherein the blood sample diluting method includes a collective blood sample diluting method and an emergency blood sample diluting method;

the collective blood sample dilution method comprises the following steps:

the emergency blood sample dilution method comprises the following steps:

placing the blood collection tube into an emergency treatment frame;