CN219320307U - Detection equipment for fluorescence immunoassay - Google Patents
Detection equipment for fluorescence immunoassay Download PDFInfo
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- CN219320307U CN219320307U CN202223148471.6U CN202223148471U CN219320307U CN 219320307 U CN219320307 U CN 219320307U CN 202223148471 U CN202223148471 U CN 202223148471U CN 219320307 U CN219320307 U CN 219320307U
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Abstract
The utility model relates to the technical field of medical equipment, in particular to detection equipment for fluorescence immunoassay, which comprises a bottom plate, wherein a sample module, a cartridge clip module, an incubation module, a photoelectric data acquisition module and a puncture sampling module are integrated on the bottom plate, and a microfluidic disc module integrated on the bottom plate is configured to bear a microfluidic disc and drive the microfluidic disc to rotate, wherein the microfluidic disc comprises a whole blood centrifugal bin, a plasma bin and a blood cell sedimentation bin, sealing films are covered on the upper side and the lower side of the microfluidic disc, and a sample feeding port is arranged on the upper surface of the whole blood centrifugal bin. The utility model integrates the microfluidic disc into the detection equipment of fluorescence immunoassay, realizes direct loading of whole blood sample, realizes in-machine sample centrifugation, reduces manual operation, and ensures consistency of detection results.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to detection equipment for fluorescence immunoassay.
Background
Immunoassay is an analysis technique based on characteristic reactions of antigens and antibodies, and is mainly classified into radioimmunoassay, enzyme immunoassay, chemiluminescent immunoassay, fluorescent immunoassay, etc., according to the difference of labeling technique means. The existing immunity analyzer is roughly divided into two types, namely equipment with low automation degree, the first type is equipment with low automation degree, the project detection is flexible, the complicated manual steps are needed for auxiliary sample adding detection, the detection result is inaccurate and the detection efficiency is low due to manual intervention, and the other type is equipment with high automation degree, and the equipment has the advantages of high detection efficiency and accurate result.
The quantitative result is more accurate when the fluorescent immunoassay instrument detects that the sample is serum or plasma, and the sample is required to be centrifuged out by a centrifuge before the sample is put on the machine, so that the operation steps are complicated.
For example, china has turned to and has disclosed a immunoassay detection device and detection method of application CN115184592A, it is a technical field of medical auxiliary equipment; the device comprises a bottom plate, wherein a sample module, a consumable module, a cartridge clip module, an incubation module, a photoelectric data acquisition module, a sampling module and an emergency detection module are arranged on the bottom plate. The sampling module takes samples extracted by the sample module and uniformly mixes the samples with the consumable module, the cartridge clip module provides reagent cards, the reagent cards are pushed into a rotary table of the incubation module through a card pushing mechanism of the incubation module, the sampling module drops the uniformly mixed samples onto the reagent cards for incubation, and the rotary table is rotated to the card returning card pushing mechanism to push the reagent cards to the photoelectric data acquisition module for scanning and then push the reagent cards to the waste box.
For example, chinese patent application CN104535782a discloses a full-automatic fluorescence immunoassay device and a realization method, the immunoassay device comprises a supporting base plate, a reagent strip storage and automatic loading module, a reaction tray module, a detection module, a sample adding module, a washing module and a control system, the reagent strip storage and automatic loading module, the reaction tray module, the detection module, the sample adding module and the washing module are sequentially arranged on the supporting base plate, the reagent strip storage and automatic loading module provides the reagent strip for the reaction tray module, the sample adding module adds the sample in the sample module into the reaction tray module for reaction, and the sample enters the detection module for detection after the reaction is completed.
In the scheme in the prior art, sample centrifugation cannot be realized in the fluorescent immunoassay instrument, and the sample is required to be centrifuged out of plasma by a centrifuge before on-machine test, so that the operation is complex.
Disclosure of Invention
In order to solve the technical problem that the detection equipment for fluorescent immunoassay in the prior art cannot realize the in-machine centrifugation of a sample, one embodiment of the utility model provides detection equipment for fluorescent immunoassay, which comprises a bottom plate, wherein a sample module, a cartridge clip module, an incubation module, a photoelectric data acquisition module and a puncture sampling module are integrated on the bottom plate,
and a microfluidic disc module integrated on the base plate, the microfluidic disc module configured to carry a microfluidic disc and drive the microfluidic disc to rotate,
the microfluidic disc comprises a whole blood centrifugal bin, a plasma bin and a blood cell precipitation bin, wherein sealing films are covered on the upper side and the lower side of the microfluidic disc, and a sample adding port is formed in the upper surface of the whole blood centrifugal bin.
In a preferred embodiment, the microfluidic disc module comprises a cartridge, which carries the microfluidic disc therein.
In a preferred embodiment, the microfluidic disc module further comprises a mixing plate and a diluent bottle, the diluent bottle containing a diluent.
In a preferred embodiment, the microfluidic disc module further comprises a mounting plate, a driving motor is mounted below the mounting plate, and an output shaft of the driving motor extends into the disc bin and is used for driving the microfluidic disc to rotate.
In a preferred embodiment, the lancing sampling module includes a first mechanical arm and a second mechanical arm, the first mechanical arm being mounted on the second mechanical arm and configured to reciprocate on the second mechanical arm in a first direction;
the first robotic arm has a sampling needle mounted thereon, the sampling needle configured to reciprocate on the first robotic arm in a second direction.
In a preferred embodiment, the cartridge module includes a plurality of reagent card slots for receiving reagent cartridges;
the bottom plate is opposite to the reagent card slot, a first card pushing mechanism and a second card pushing mechanism are arranged, and the first card pushing mechanism is used for pushing the reagent card in the reagent card box into the incubation module;
the second card pushing mechanism is used for pushing the reagent card out of the incubation module.
In a preferred embodiment, the incubation module includes a carousel defining a plurality of channels for carrying reagent cards.
In a preferred embodiment, a heat-insulating cover is arranged above the turntable.
In a preferred embodiment, the sample module comprises a support plate and a blood collection stand, the blood collection stand being inserted into the sample module through the support plate,
the blood sampling frame is provided with a blood sampling tube.
In a preferred embodiment, the sample module further comprises a sensor located on an opposite side of the lancing rack.
The technical scheme provided by the embodiment of the utility model has the beneficial effects that at least:
the utility model provides a detection device for fluorescence immunoassay, which integrates a microfluidic disc into the detection device for fluorescence immunoassay, so that direct loading of a whole blood sample is realized, in-machine sample centrifugation is realized, manual operation is reduced, and the consistency of detection results is ensured. Compared with the detection flow of the traditional fluorescent immunoassay detection equipment, the method is more convenient, faster and more accurate, and realizes quantitative detection of the sample.
The utility model provides detection equipment for fluorescence immunoassay, which is used for centrifuging whole blood in a machine to obtain plasma/serum, solves the problem of pretreatment links of sample detection (sample loading is needed to be put into a centrifugal machine for centrifugation, and the accurate centrifugation of a microfluidic disc is realized, so that the whole blood sample is directly loaded, the detection flow is more convenient, and the detection result is more accurate.
The utility model provides detection equipment for fluorescence immunoassay, a user can realize plasma/serum sample loading, quantitative detection and high accuracy by only inserting a detected sample into a sample module, and the whole blood can separate plasma, so that the detection equipment is matched with full-automatic detection and liberates hands of operators. The utility model performs puncture sampling without cap pulling and other operations.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram showing the overall structure of a detection apparatus for fluorescence immunoassay of the present utility model.
FIG. 2 is a schematic diagram showing the structure of a bottom plate and an incubation module of a detection apparatus for fluorescence immunoassay according to the present utility model.
Fig. 3 is a schematic structural diagram of a microfluidic disc module of a detection apparatus for fluorescent immunoassay according to the present utility model.
Fig. 4 is a schematic structural view of a mounting plate of the microfluidic disc module of the present utility model.
Fig. 5 is a schematic structural view of a microfluidic disc according to the present utility model.
FIG. 6 is a schematic diagram of the structure of a sample block of a detection apparatus for fluorescence immunoassay of the present utility model.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The terms "first," "second," "third," "fourth" and the like in the description and in the claims and in the above drawings, if any, are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the utility model described herein may be implemented, for example, in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The overall structure of the detection device for fluorescent immunoassay of the present utility model as shown in fig. 1, and the structure of the bottom plate and the incubation module of the detection device for fluorescent immunoassay of the present utility model as shown in fig. 2, according to an embodiment of the present utility model, a detection device for fluorescent immunoassay is provided, which comprises a bottom plate 1, and a sample module 2, a microfluidic disc module 3, a cartridge clip module 4, an incubation module 5, a photoelectric data acquisition module 6, and a puncture sampling module 7 are integrated on the bottom plate 1.
According to an embodiment of the utility model, the puncture sampling module 7 is located behind the microfluidic disc module 3. The puncture sampling module 7 includes a first mechanical arm 703 and a second mechanical arm 702. The first robot arm 703 is mounted on the second robot arm 702, and is configured to reciprocate on the second robot arm 702 in a first direction (a direction indicated by an arrow a in fig. 1). In some embodiments, the first robotic arm 703 may be reciprocally moved in a first direction on the second robotic arm 702 by way of a drag chain drive. In other embodiments, the first robot 703 may reciprocate on the second robot 702 in a first direction by means of belt conveyance.
According to an embodiment of the present utility model, the sampling needle 701 is mounted on the first robot arm 703, and the sampling needle 701 is configured to reciprocate on the first robot arm 703 in the second direction (the direction indicated by the arrow b in fig. 1). In some embodiments, the reciprocating movement of the sampling needle 701 in the second direction on the first robotic arm 703 may be accomplished by a drag chain drive. In other embodiments, the reciprocating movement of the sampling needle 701 in the second direction on the first robot arm 703 may be achieved by means of a belt conveyor.
According to an embodiment of the present utility model, the sampling needle 701 is configured to be reciprocally movable in a vertical direction, for example, by driving the sampling needle 701 to reciprocally move in the vertical direction by a telescopic motor.
According to an embodiment of the utility model, the cartridge module 4 is located on one side of the microfluidic disc module 3 and the sample module 2 is located on the other side of the microfluidic disc module 3.
The cartridge module 4 includes a plurality of reagent card slots 401 for receiving reagent cartridges (not shown). The first card pushing mechanism 11 and the second card pushing mechanism 12 are arranged at positions of the bottom plate 1 opposite to the reagent card slot 401. The code scanner 13 is arranged on the bottom plate 1. A first card pushing mechanism 11 for pushing stacked reagent cards in the reagent cartridge into the incubation module 5. And the second card pushing mechanism 12 is used for pushing the reagent card detected by the incubation module 5 out of the incubation module 5.
According to an embodiment of the utility model, an incubation module 5 is arranged behind the cartridge holder module 4, the incubation module 5 comprises a turntable 502, and the turntable 502 is provided with a plurality of channels 503 for carrying reagent cards. When the first card pushing mechanism 11 pushes the stacked reagent cards in the reagent cartridge into the incubation module 5, the reagent cards are caught in the turntable 502 through the passage 503. When the detection is completed, the turntable 502 rotates (in the direction indicated by arrow c in fig. 2) until the reagent card is facing the second card pushing mechanism 12, and the second card pushing mechanism 12 pushes the reagent card in the channel 503 of the incubation module 5 out of the incubation module 5.
In a further preferred embodiment, a heat-insulating cover 501 is arranged above the turntable 5, and the heat-insulating cover 501 covers the channel 503 of the turntable 502 to insulate the reagent card.
The structure of a microfluidic disc module of the detection apparatus for fluorescent immunoassay of the present utility model as shown in fig. 3, the structure of a mounting plate of the microfluidic disc module of the present utility model as shown in fig. 4, the structure of a microfluidic disc of the present utility model as shown in fig. 5, and the microfluidic disc module 3 integrated on the base plate 1 according to the embodiment of the present utility model is configured to carry the microfluidic disc 301 and drive the microfluidic disc 301 to rotate.
The microfluidic disc module 3 comprises a cartridge in which the microfluidic disc 301 is carried. Specifically, the microfluidic disc module 3 includes a mounting plate 304, and a disc cartridge is provided on the mounting plate 304, and the microfluidic disc 301 is disposed in the disc cartridge. A driving motor 302 is arranged below the mounting plate 304, and an output shaft of the driving motor 302 extends into the disc bin and is used for driving the microfluidic disc 301 to rotate.
According to an embodiment of the present utility model, the microfluidic disc module 3 further includes a mixing plate 307 and a diluent bottle 308, the diluent bottle 308 containing a diluent, and the diluent tube 308 is located at one side of the mixing plate 307. In a specific embodiment, the mounting plate 304 is provided with a consumable middle plate 303, and the consumable middle plate 303 is provided with a mixing plate 307.
In a preferred embodiment, the microfluidic disc module 3 further comprises a disc cover 306, wherein a pushing handle 305 is provided on the disc cover 306, and the disc cover 306 is covered over the microfluidic disc 301 by pushing the pushing handle 305. Above the disc cover 306 there is a through channel for the sampling needle 701 to add whole blood samples or to collect plasma.
According to an embodiment of the present utility model, the microfluidic disc 301 includes a whole blood centrifugation chamber 3011, a plasma chamber 3012, and a blood cell sedimentation chamber 3013. The whole blood centrifugation chamber 3011 is close to the center of the microfluidic disc 301, the plasma chamber 3012 is located outside the whole blood centrifugation chamber 3011, and the blood cell sedimentation chamber 3013 is located outside the plasma chamber 3012. Sealing films are covered on the upper side and the lower side of the micro-flow hole disc 301, and a sample adding port 3014 is arranged on the upper surface of the whole blood centrifugal bin 3011 and used for adding whole blood samples into the whole blood centrifugal bin 3011.
In a preferred embodiment, the sealing materials on the upper and lower sides of the micro-hole disk 301 include, but are not limited to, PS, ABS, PP, PE, PVC, PMMA, PC, PC +abs.
In a preferred embodiment, the upper and lower sides of the micro-hole disk 301 are covered with sealing films using a bonding process, which includes, but is not limited to, hot pressing, bonding, ultrasonic welding, laser welding.
As shown in fig. 6, a sample module 2 of a detection apparatus for fluorescent immunoassay according to an embodiment of the present utility model includes a support plate 202 and a blood collection stand 201, a slide rail is provided on the support plate 202, and the blood collection stand 201 is inserted into the sample module 2 through the slide rail on the support plate 202. The blood collection tube 204 is carried on the blood collection stand 201.
The sample module 2 further comprises a sensor 203, the sensor 203 being located on the opposite side of the lancing frame 201. When the blood sampling frame 201 is inserted into the sample module 2 through the slide way on the supporting plate 202, the sensor 203 senses that the blood sampling frame 201 is inserted into the sample module 2, and the code scanner 13 on the bottom plate 1 inputs one-dimensional code information of the blood sampling frame 201.
The following describes a detection procedure of a detection apparatus for fluorescent immunoassay of the present utility model.
After the blood sampling rack 201 on the sample module 2 is inserted into the sample module 2, the sensor 203 installed on the opposite side of the blood sampling rack 201 senses that the blood sampling rack 201 is inserted into the sample module 2, and the code scanner 13 installed on the bottom plate 1 inputs one-dimensional code information on the blood sampling rack 201, so that the detection sample position and patient information corresponding to the blood sampling tube 204 on the blood sampling rack 201 are determined.
After the detection project is recorded, the puncture needle 701 on the puncture sampling module 7 moves to the position above the blood collection tube 204, the sampling needle 701 moves downwards, the tube cap on the blood collection tube 204 is inserted, and the whole blood sample in the blood collection tube 204 is collected.
The sampling needle 701 moves upward away from the exploitation blood vessel 204 and moves to above the microfluidic disc module 3, the sampling port 3014 is opposite to the upper surface of the whole blood centrifugation chamber 3011 of the microfluidic disc 301, the sampling needle 701 moves downward to be inserted into the sampling port 3014 on the upper surface of the whole blood centrifugation chamber 3011, the whole blood sample is added to the whole blood centrifugation chamber 3011 of the microfluidic disc 301, and the sampling needle 701 moves upward away from the microfluidic disc 301.
The drive motor 302 drives the microfluidic disc 301 to operate centrifugally at a rotational speed n1r/min for a time period t1 s. The microfluidic disc 301 is based on centrifugal force, and achieves the function of a 'lab-on-a-chip' by the aid of capillary force, siphoning action and the like, so that the whole blood separation function in the whole blood centrifugal bin 3011 is realized. After centrifugation, the plasma/serum is distributed to the plasma chamber 3012 through the flow channel in the microfluidic disc 301, and the blood cells enter the blood cell precipitation chamber 3013.
At the same time, one reagent card of the reagent cassettes in the reagent card slot 401 of the cartridge clip module 4 is pushed into the channel 503 opened by the turntable 502 of the incubation module 5 by the first card pushing mechanism 11 mounted on the bottom plate 1.
The sampling needle 701 moves to face the plasma bin 3012 of the microfluidic disc 301, the sampling needle 701 moves downwards, and the upper side sealing film of the microfluidic disc 301 is pierced to be inserted into the plasma bin 3012 to collect plasma/serum.
The sampling needle 701 moves upward, away from the microfluidic disc 301, and moves directly above the reagent card that snaps into the channel 503 of the turntable 502, and the sampling needle 701 moves downward to insert the reagent card, adding plasma/serum into the reagent card for reaction.
In some embodiments, dilution is required after the sampling needle 701 collects plasma/serum. The specific process is as follows: the sampling needle 701 moves upward after collecting plasma/serum, leaves the microfluidic disc 301, and moves to just above the mixing plate 307, and the sampling needle 701 moves downward to insert into the mixing plate 307, adding plasma/serum into the mixing plate 307.
The sampling needle 701 moves upward away from the mixing plate 307 and up to just above the diluent bottle 308, and the sampling needle 701 moves downward to insert the diluent bottle 308 to collect the diluent.
The sampling needle 701 moves upward away from the diluent bottle 308 and to just above the mixing plate 307, the sampling needle 701 moves downward into the mixing plate 307, diluent is added into the mixing plate 307, and the sampling needle 701 moves upward away from the mixing plate 307. The plasma/serum is mixed with the diluent in a mixing plate 307.
After the plasma/serum is diluted by mixing with the diluent, the sampling needle 701 is inserted into the mixing plate 307 to collect the diluted plasma/serum. The sampling needle 701 moves upward away from the mixing plate 307 and moves to just above the reagent card that is snapped into the channel 503 of the turntable 502, and the sampling needle 701 moves downward to insert the reagent card, adding plasma/serum into the reagent card for reaction.
After the reaction is completed, the photoelectric data acquisition module 6 performs data acquisition and completes detection. After the detection is completed, the turntable 502 rotates (in the direction indicated by the arrow c in fig. 2) until the reagent card is opposite to the second card pushing mechanism 12, and the second card pushing mechanism 12 pushes the reagent card in the channel 503 of the incubation module 5 out of the incubation module 5.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the utility model.
Claims (10)
1. A detection device for fluorescence immunoassay is characterized by comprising a bottom plate, wherein a sample module, a cartridge clip module, an incubation module, a photoelectric data acquisition module and a puncture sampling module are integrated on the bottom plate,
and a microfluidic disc module integrated on the base plate, the microfluidic disc module configured to carry a microfluidic disc and drive the microfluidic disc to rotate,
the microfluidic disc comprises a whole blood centrifugal bin, a plasma bin and a blood cell precipitation bin, wherein sealing films are covered on the upper side and the lower side of the microfluidic disc, and a sample adding port is formed in the upper surface of the whole blood centrifugal bin.
2. The detection apparatus according to claim 1, wherein the microfluidic disc module comprises a cartridge that carries the microfluidic disc therein.
3. The detection apparatus of claim 2, wherein the microfluidic disc module further comprises a mixing plate and a diluent bottle, the diluent bottle containing diluent.
4. The detection apparatus according to claim 2, wherein the microfluidic disc module further comprises a mounting plate, a driving motor is mounted below the mounting plate, and an output shaft of the driving motor extends into the disc cartridge for driving the microfluidic disc to rotate.
5. The test device of claim 1, wherein the lancing sampling module includes a first robotic arm and a second robotic arm, the first robotic arm mounted on the second robotic arm and configured to reciprocate on the second robotic arm in a first direction;
the first robotic arm has a sampling needle mounted thereon, the sampling needle configured to reciprocate on the first robotic arm in a second direction.
6. The test device of claim 1, wherein the cartridge module comprises a plurality of reagent card slots for receiving reagent cartridges;
the bottom plate is opposite to the reagent card slot, a first card pushing mechanism and a second card pushing mechanism are arranged, and the first card pushing mechanism is used for pushing the reagent card in the reagent card box into the incubation module;
the second card pushing mechanism is used for pushing the reagent card out of the incubation module.
7. The detection apparatus according to claim 1 or 6, wherein the incubation module comprises a carousel, the carousel defining a plurality of channels for carrying reagent cards.
8. The inspection apparatus of claim 7 wherein a thermal cover is disposed over the turntable.
9. The test device of claim 1, wherein the sample module comprises a support plate and a lancing rack, the lancing rack being inserted into the sample module through the support plate,
the blood sampling frame is provided with a blood sampling tube.
10. The test device of claim 9, wherein the sample module further comprises a sensor located on an opposite side of the lancing rack.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223148471.6U CN219320307U (en) | 2022-11-23 | 2022-11-23 | Detection equipment for fluorescence immunoassay |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223148471.6U CN219320307U (en) | 2022-11-23 | 2022-11-23 | Detection equipment for fluorescence immunoassay |
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| CN219320307U true CN219320307U (en) | 2023-07-07 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117233412A (en) * | 2023-11-13 | 2023-12-15 | 成都斯马特科技有限公司 | Microfluidic biochemical reagent disk and biochemical inspection and analysis method |
| CN117517640A (en) * | 2023-10-30 | 2024-02-06 | 苏州思迈德生物科技有限公司 | Sample analyzer, control system and control method of sample analyzer |
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2022
- 2022-11-23 CN CN202223148471.6U patent/CN219320307U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117517640A (en) * | 2023-10-30 | 2024-02-06 | 苏州思迈德生物科技有限公司 | Sample analyzer, control system and control method of sample analyzer |
| CN117517640B (en) * | 2023-10-30 | 2024-05-24 | 千深科技(苏州)有限公司 | Sample analyzer, control system and control method of sample analyzer |
| CN117233412A (en) * | 2023-11-13 | 2023-12-15 | 成都斯马特科技有限公司 | Microfluidic biochemical reagent disk and biochemical inspection and analysis method |
| CN117233412B (en) * | 2023-11-13 | 2024-02-02 | 成都斯马特科技有限公司 | Microfluidic biochemical reagent disk and biochemical inspection and analysis method |
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