CN111323411B - Multi-project combined chemiluminescent instant detection system - Google Patents
Multi-project combined chemiluminescent instant detection system Download PDFInfo
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- CN111323411B CN111323411B CN202010204315.3A CN202010204315A CN111323411B CN 111323411 B CN111323411 B CN 111323411B CN 202010204315 A CN202010204315 A CN 202010204315A CN 111323411 B CN111323411 B CN 111323411B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses a multi-project combined chemiluminescence instant detection system, which comprises: a kit having a plurality of reagent sites required for the assay; an instant detection system comprising: the center of the lower part of the reagent disk bracket is connected with a reagent disk rotating shaft; the kit comprises a liquid transferring mechanism for transferring liquid, a detection mechanism for collecting luminous information of a reaction system in the kit, and a plurality of communicated reaction sites, wherein detection separation strips are arranged in the reaction sites, the bottoms of the detection separation strips are closed, and the upper parts of the detection separation strips are communicated; the instant detection system also comprises a cleaning mechanism and a mixing mechanism which are applicable to the reaction position; the mixing mechanism is arranged below the kit platform. According to the invention, the bottoms of the reaction cups are used as carriers, the reaction cups are communicated with each other, antibodies or antigens are marked on the bottoms of the reaction cups, and after the reagents are added, the reagents flow back and forth in each reaction cup through the mixing mechanism, so that the reagents are fully reacted, the detection accuracy is improved, meanwhile, a plurality of items can be marked on the bottoms of the reaction cups at the same time, the items can be detected at the same time under the condition of a small amount of sample, and the time for outputting results is shortened.
Description
Technical Field
The invention relates to the technical field of immunodiagnosis detection systems, in particular to a multi-item combined chemiluminescence instant detection system.
Background
At present, immunoassay is an important means for clinical diagnosis, and a main immunoassay method is a chemiluminescence method, has the characteristics of sensitivity, stability and the like, and is widely applied to clinical detection. Although the chemiluminescence advantages are obvious compared with the prior immunoassay method, most of the immunoassay methods adopt a magnetic bead coating system, the experiment has a magnetic bead cleaning link, and the magnetic beads are easy to lose, so that the accuracy of the experimental result can be influenced. In addition, the traditional test method generally cannot output results from multiple projects at the same time, namely, the high-flux instrument can be completed in a plurality of hours, and the test is generally carried out in a specific laboratory environment, so that the test method is not easy to be used for rapid detection in the field or beside a bed.
Meanwhile, the prior art discloses a chemiluminescent instant detection system, which specifically discloses a chemiluminescent instant detection system comprising: a kit having a plurality of reagent sites required for the assay; the immediate detection system comprises a reagent disk bracket for bearing a reagent kit, and the center of the lower part of the reagent disk bracket is connected with a reagent disk rotating shaft; the device is provided with a liquid transferring mechanism for transferring liquid and a detection mechanism for collecting luminous information of a reaction system in the kit, but the device is provided with a plurality of communicated reaction positions aiming at the kit, and the adaptive equipment for detecting the separation strips is arranged in the reaction positions, so that the device is further improved on the basis of the detection system, the mixing uniformity among the reaction cups after the test is further improved, the full reaction is obtained, and the accuracy is improved.
Disclosure of Invention
Aiming at the defects existing in the background, the invention aims to overcome the defects existing in the prior art that a detection system coated by magnetic beads is needed, the bottoms of the reaction cups are used as carriers, a plurality of reaction cups are communicated with each other, antibodies or antigens are marked on the bottoms of the reaction cups, after reagents are added, the reagents flow back and forth in each reaction cup through a mixing mechanism, so that the reagents fully react, the magnetic beads are not needed as the carriers, the cleaning links with the difference caused by the loss or the uncleanness of the magnetic beads are avoided, the detection accuracy is improved, a plurality of items can be marked on the bottoms of the reaction cups at the same time, the simultaneous detection of the items can be carried out under the condition of a small amount of sample, and the time for outputting results is shortened.
In order to achieve the above object, the present invention is realized by the following technical scheme:
a multi-project joint chemiluminescent instant detection system comprising:
a kit having a plurality of reagent sites required for the assay;
an instant detection system comprising: the center of the lower part of the reagent disk bracket is connected with a reagent disk rotating shaft; the detection device is provided with a liquid transferring mechanism for transferring liquid and a detection mechanism for collecting luminous information of a reaction system in the kit;
the kit also comprises a plurality of reaction sites which are communicated, wherein detection separation strips are arranged in the reaction sites, the bottoms of the detection separation strips are closed, and the upper parts of the detection separation strips are communicated; the instant detection system also comprises a cleaning mechanism and a mixing mechanism which are applicable to the reaction position; the mixing mechanism is arranged below the kit platform.
Optionally, the kit is a disposable film sealing kit; the disposable film sealing kit comprises a kit body and a film sealing arranged on the kit body; the box body at least comprises a reagent position and at least one reaction position; the reagent positions comprise a sample position, a mixing position and a pipetting head position; the reaction bits include dense light detection bits.
Optionally, wiper mechanism locates the kit platform top, through slide rail assembly and the backplate fixed connection of kit platform top, and the wiper assembly includes:
the sliding plate is L-shaped, wherein the vertical side wall is connected with the sliding rail assembly to realize up-and-down sliding, and the horizontal side wall is provided with a plurality of through holes;
the motion module comprises a screw motor and drives the sliding plate to slide up and down;
the liquid sucking and discharging module comprises a plurality of cleaning needles vertically penetrating through holes on the sliding plate, an elastic body is arranged at the upper part of each cleaning needle, a needle baffle is fixedly arranged on the sliding plate, a plurality of through holes matched with the cleaning needles are formed in each needle baffle, and the elastic body can be stretched and compressed when the cleaning needles vertically slide through the upper parts of the cleaning needles;
the pushing module comprises a pushing fixing piece which is L-shaped, wherein the vertical side wall is fixedly arranged at the front end of the horizontal side wall of the sliding plate, a pressing block fixing through hole is formed in the horizontal side wall, and the pressing block assembly is elastically connected with the horizontal side wall of the pushing fixing piece.
Optionally, the briquetting subassembly includes briquetting and the support column of being connected with briquetting upper surface an organic whole, and the cover is equipped with the elastomer on the support column, and the through-hole on the mounting is pushed down in the upper portion of support column and sliding connection.
Optionally, the cleaning needle comprises a liquid suction needle and a liquid injection needle which are arranged in parallel at the bottom, a needle connecting block is fixedly arranged at the upper part of the liquid suction needle and the upper part of the liquid injection needle, the liquid injection needle is driven by an eight-way peristaltic pump, and the waste liquid needle is driven by a direct current pump.
Optionally, the lower part of lead screw motor is equipped with the motor fixed block, and the lateral wall of motor fixed block is equipped with a photoelectric sensor, be equipped with photoelectric separation blade on the sliding plate, the sliding plate is gliding in-process from top to bottom, and photoelectric sensor senses photoelectric separation blade, and the status signal that photoelectric sensor produced has the level height to change.
Optionally, the mixing mechanism includes:
the mixing bearing is tightly arranged on the rotating shaft of the reagent disk;
the mixing rail is annular, is sleeved on the rotating shaft of the reagent disk and is arranged above the mixing bearing, and the annular side wall of the mixing rail is a slope with a curve shape;
the driving assembly comprises a stepping motor, the stepping motor moves to drive the synchronous belt pulley to rotate, and the synchronous belt pulley drives the synchronous belt to move, so that the synchronous belt uniformly mixes with the guide rail to rotate;
the kit mixing backup pad, annular, cover are located the reagent dish pivot and are arranged in mixing track upper portion, and its lower surface is equipped with mixing gyro wheel, mixing gyro wheel is at mixing track slope up-and-down motion is realized through upper and lower position change for kit mixing backup pad.
Optionally, the mixing roller moves on a slope of the mixing track to drive the mixing angle of the kit to be 0-15 degrees.
Optionally, the even backup pad of kit mixes with the upper end swing joint at two guiding axles of symmetry setting, and the guiding axle slides from top to bottom in the guiding axle sleeve, and the spring sets up in the lower part of axle sleeve so that the guiding axle has decurrent driving force.
Optionally, the kit is placed on the kit collet, and the kit collet sets up on the reagent dish support, and reagent dish support bottom has set gradually insulating film, hot plate and shock attenuation piece from last to down, is equipped with the kit gyro wheel in the nearly middle part of shock attenuation piece lower part, and the nearly outer fringe department of shock attenuation piece lower part is equipped with torsional spring subassembly.
The technical scheme of the invention has the following advantages:
the disposable film sealing kit with a plurality of reagent positions required by the test and the multi-project combined chemiluminescence instant detection system provided by the embodiment of the invention comprise a liquid transferring mechanism for transferring liquid, a mixing mechanism for fully reacting reagents in a plurality of reaction cups, a detection mechanism for collecting luminous information of a reaction system in the kit and a cleaning mechanism for cleaning; the reaction cups are communicated, and after the reagent is added, the reagent flows back and forth in each reaction cup through the mixing mechanism, so that the reagent fully reacts. The structure avoids using magnetic beads as carriers for adsorbing and combining various molecules, and simultaneously, the kit and the pipetting head are made of disposable materials, so that test errors and cross contamination of reagents and strains are reduced to a great extent, and the accuracy of a test is improved.
The multi-project combined chemiluminescence instant detection system provided by the embodiment of the invention uses the disposable pipetting head and the kit as carriers for transferring and storing liquid, and the pipetting head can be recycled after the test, so that chemical pollution is reduced; the kit is provided with a plurality of functional areas, one sample corresponds to one test box, and no extra consumable is added; the whole instrument has compact structure, is small and portable, and is suitable for on-site detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of a platform structure of the kit of the present invention;
FIG. 3 is a schematic view of a pipetting mechanism of the invention;
FIG. 4 is a schematic diagram of the structure of the kit of the present invention;
FIG. 5 is a schematic structural diagram of a reaction site;
FIG. 6 is a schematic diagram of the structure of a test separator;
FIG. 7 is a schematic view of a cleaning mechanism according to the present invention;
FIG. 8 is a schematic view of an assembly of a cleaning mechanism according to the present invention;
FIG. 9 is a schematic view of the structure of a combination needle;
FIG. 10 is a schematic diagram of a blending structure according to the present invention;
FIG. 11 is a schematic view of the mixing rail structure of FIG. 10;
FIG. 12 is a schematic diagram of a kit shoe assembly.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Example 1
The multi-item combined chemiluminescence instant detection system provided by the embodiment comprises a disposable film sealing kit and the multi-item combined chemiluminescence instant detection system. Referring to fig. 1, the chemiluminescent instant detection system comprises a kit platform 1 for carrying a kit, a pipetting mechanism 2 for transferring liquid, a cleaning mechanism 5 for cleaning, a detection mechanism 4 for collecting luminescent information of a reaction system in the kit and a mixing mechanism.
Specifically, referring to fig. 2, the kit platform 1 is in a rotatable disc structure, the kit platform 1 comprises a kit bracket 14, the kit bracket 14 can be matched with a kit, the kit is placed on the kit bracket 14, twelve kits 11 can be carried and transferred on the kit platform according to the embodiment, and the kits 11 can be freely taken and placed from the kit platform; the kit platform 1 is also provided with a temperature control module for providing incubation reaction temperature and a kit mixing mechanism, the kit platform movement module can control the kit 11 to rotate on the disc, and the kit platform movement module is matched with the pipetting mechanism 2 to finish pipetting and mixing operations and the detection mechanism 4 to finish detection of a kit reaction system; referring to fig. 3, the pipetting mechanism 2 at least comprises a pipetting assembly, a motion platform, a slide rail control module 21 for controlling the pipetting assembly to move linearly horizontally or vertically, and a screw motion module 22; the motion platform can at least do two-dimensional motion in a plane vertical to the reagent box platform, and can also consist of a three-dimensional motion mechanism. The pipetting component can be fixed on the moving platform and can move in two or three dimensions along with the moving platform, and comprises a piston mechanism 3 combined with the pipetting component and a plunger head elastically connected with the lower end of the piston mechanism 3, and the bottom end of the pipetting component can be connected with the disposable pipetting head. The piston mechanism 3 comprises a piston rod and a cavity, a guide piece is arranged outside the piston, the piston rod can be driven by a motor to move up and down, and the piston rod expands or compresses air, and is matched with a pipetting head connected with a pipetting assembly, so that pipetting operation can be completed. Specifically, the pipetting mechanism 2 is composed of a pipetting assembly, a screw rod motion module 22 and a slide rail motion module 21, the piston mechanism 3 is connected with the screw rod motion module 22, the piston mechanism 3 can descend into a pipetting head 116 on the kit under the control of the screw rod motion module 22, and the rigid connector 32 is tightly matched with the top end of the pipetting head, so that the rigid connector 32 can ensure the air tightness of the structure. The pipetting head is driven by the screw motion module 22 to perform linear motion in the vertical direction. The screw rod movement module 22 is connected with the slide rail movement module 21, and the screw rod movement module 22 can move in the horizontal direction under the control of the slide rail movement module 21. The piston mechanism 3 is composed of a piston and a piston motion control module 31, one end of the piston is inserted into the hard connector 32, and is connected with the hard connector 32 through a sealing ring positioned at the top of the hard connector 32 and keeps air tightness. The other end of the piston is connected with a piston motion control module 31, which controls the linear motion of the piston in the vertical direction. In an embodiment, the piston motion control module 31 is coupled to the control piston. When the pipetting head is obtained, the reagent box platform 1 drives the reagent box 11 to move, the hard connector 32 moves to the vertical upper part of the pipetting head by the sliding rail movement module 21 in the pipetting mechanism 2, then the hard connector 32 is lowered into the pipetting head inserted into the reagent box 11 by the screw movement module 22 in the pipetting mechanism 2, the screw movement module 22 in the pipetting mechanism 2 is raised to be separated from the reagent box 11, and the pipetting head is obtained. When liquid transfer is carried out, the reagent box platform 1 drives the reagent box 11 to move to the position right below the liquid transferring head, the liquid transferring head moves to a hole position of the reagent box 11 needing liquid transferring under the drive of the screw rod movement module 22 in the liquid transferring mechanism 2, the liquid transferring head enters the liquid of the corresponding hole position of the reagent box 11 needing liquid transferring under the drive of the screw rod movement module 22 in the liquid transferring mechanism 2, at the moment, the piston 3 moves upwards under the control of the piston movement control module 31 to enable the liquid to enter the liquid transferring head, then the screw rod movement module 22 in the liquid transferring mechanism 2 moves upwards to drive the liquid transferring head 11 to leave the reagent box 11, the slide rod movement module 21 in the liquid transferring mechanism 2 moves to the corresponding hole position of the reagent box 11 needing liquid transferring, the liquid transferring head 12 enters the corresponding position of the reagent box 11 needing liquid transferring under the drive of the screw rod movement module 22 in the liquid transferring mechanism 2, and the piston mechanism 3 moves downwards under the control of the piston movement control module 31 to enable the liquid in the liquid transferring head to be discharged to the target position, and the transfer of the liquid is completed.
The disposable film sealing kit is shown in fig. 4, and consists of a kit 11 and a detection cup; the kit 11 comprises a plurality of functional separation sites, including a sample site 112, mixing sites 110, 111, reagent sites 113, 114, a dense light detection site (reaction site) 115 and a TIP head site 116, wherein the dense light detection site (reaction site) 115 contains detection separation strips, the reagent sites 113 and 114 have a plurality of functional separation strips, and can be selected and functionally expanded according to the application, and the sample sites include, but are not limited to, a sample loading site and a sample sampling site, the sample loading site is communicated with the sample sampling site, and a filter membrane is arranged in the sample site, so that most of impurities in whole blood in the sample loading site can be filtered, and serum samples can enter the sample sampling site through the filter membrane; the box body can be pre-filled with defined reaction reagent and the top sealing film after the reaction cup; the disposable film sealing kit used for the experiment is a consumable material, and is replaced along with the completion of the experiment. The dense light detection position, see fig. 5 and 6, is formed by a plurality of reaction sites that are communicated, an 8-hole detection separation strip is arranged in the reaction sites, 8 detection holes are formed in the detection strip, the bottoms of the detection holes are closed, the lower parts of the detection holes are mutually independent, and the upper parts of the detection holes are communicated through a connecting channel.
Specifically, referring to fig. 8, a screw motor is fixed on a 51 motor fixing member 511, a guide rail slider 526 can slide on a 527 guide rail, a needle fixing member 524 is fixed on the guide rail slider 526, the needle fixing member 524 can move up and down, a screw nut 525 is fixed on the needle fixing member 524, a screw of the screw motor 51 passes through the screw nut 525, the screw motor 51 drives the needle fixing member 524 to move in an instrument manner when the screw motor 51 moves, a photoelectric blocking piece 529 is fixed on the needle fixing member 524, a photoelectric sensor 528 is fixed on the motor fixing member 511, and a signal triggered by the photoelectric blocking piece 529 to the photoelectric sensor 528 can be accurately positioned when the needle fixing member 524 moves. The cleaning needle 523 is shown in fig. 9, and is composed of a liquid suction needle, a liquid injection needle and a needle connecting block, 8 groups of 8 liquid injection needles and 8 waste liquid suction needles are provided, the liquid injection needles are driven by an 8-way peristaltic pump, any volume of cleaning liquid can be added at the same time, the waste liquid needles are driven by a direct-current pump, the cleaning needle 523 is fast and efficient, 8 waste liquid adopts an independent self-adaptive structure, the problem that the waste liquid is not sucked cleanly due to uneven reagent box can be avoided, the cleaning needle 523 can slide up and down in a through hole of the needle fixing piece 524, the spring 522 is arranged on the cleaning needle 523, the needle baffle 521 is fixed on the needle fixing piece 524, the spring 522 is pressed, and the self-adaptation of the waste liquid suction needle and the reaction bar can be realized. The pressing down module 53 comprises a division bar pressing block 531 fixing part fixed on the needle fixing part 524, a pressing block 532, a pressing block 533 and a pressing block 534 are fixed on the division bar pressing block fixing part 531, the pressing block 534 and the pressing block 533 are composed of a spring and 2 supporting columns, the supporting columns can move up and down through holes on the division bar pressing block fixing part 531, and the self-adaption of the instrument in the process of pressing the division bars can be realized. 8 annotate liquid needle and 8 inhale the waste liquid needle, annotate the liquid needle and pass through 8 peristaltic pump drives, can add arbitrary volume washing liquid simultaneously, the waste liquid needle passes through a direct current pump drive, and is quick high-efficient, 8 waste liquids adopt independent self-adaptation structure, can have avoided leading to the waste liquid to inhale unclean problem because the kit is uneven.
Referring to fig. 10, the mixing mechanism is shown, the reagent disk platform 1 is fixed on a reagent disk rotating shaft 69, a reagent disk rotating bearing 68 is tightly mounted below the reagent disk rotating shaft, a rotating shaft sleeve 67 is mounted on a reagent disk mounting plate 64, and a locking nut 65 is fixed on the reagent disk mounting plate 64; the mixing bearing 66 is tightly arranged above the reagent disk rotating shaft 69, the mixing guide rail 624 is arranged on the outer side of the mixing bearing 66, the synchronous pulley 626 is arranged on the stepping motor 627, the stepping motor 627 moves to drive the synchronous pulley 626 to move, the synchronous pulley 626 drives the synchronous belt 625 to move, the synchronous belt 625 drives the mixing guide rail 624 to move, a slope is arranged on the mixing track 624, and particularly, as shown in fig. 11, the mixing roller 623 moves on the track slope to change the position, so that the reagent box mixing support moves up and down, the reagent box mixing support is fixed on two guide shafts 651, the guide shafts 651 can slide up and down in the guide shaft sleeves 652, and the spring 653 can enable the guide shafts 651 to have downward driving force; the kit is placed on the kit collet, and kit gyro wheel 621 is fixed in the inboard of kit collet bottom, and kit gyro wheel 621 can be in the motion of kit mixing backup pad 622, and when the up-and-down motion was supported in the kit mixing, kit gyro wheel 621 also up-and-down motion, the up-and-down motion is realized to the kit follow-up, reaches the mixing mesh.
Referring to fig. 12, the kit is placed on a kit holder 71, the kit holder 71 is disposed on a kit tray support, an insulating film 72, a heating plate 74 and a shock absorbing sheet 73 are sequentially disposed at the bottom of the kit tray support from top to bottom, a kit roller is disposed in the middle of the lower portion of the shock absorbing sheet 73, a torsion spring assembly 75 is disposed at the outer edge of the lower portion of the shock absorbing sheet, and the torsion spring is connected with a torsion spring fixing block through a torsion spring shaft.
The chemiluminescent detection system can also comprise a liquid crystal display screen and a printer, wherein the liquid crystal display screen can perform experimental setting and result display, and the printer can perform result printing.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (7)
1. A multi-project joint chemiluminescent instant detection system comprising:
a kit having a plurality of reagent sites required for the assay;
an instant detection system comprising: the center of the lower part of the reagent disk bracket is connected with a reagent disk rotating shaft; the kit is characterized by comprising a liquid transferring mechanism for transferring liquid and a detection mechanism for collecting luminous information of a reaction system in the kit,
the kit also comprises a plurality of reaction sites which are communicated, wherein detection separation strips are arranged in the reaction sites, the bottoms of the detection separation strips are closed, and the upper parts of the detection separation strips are communicated; the instant detection system also comprises a cleaning mechanism and a mixing mechanism which are applicable to the reaction position; the mixing mechanism is arranged below the kit platform; the mixing mechanism includes:
the mixing bearing is tightly arranged on the rotating shaft of the reagent disk;
the mixing rail is annular, is sleeved on the rotating shaft of the reagent disk and is arranged above the mixing bearing, and the annular side wall of the mixing rail is a slope with a curve shape;
the driving assembly comprises a stepping motor, the stepping motor moves to drive the synchronous belt pulley to rotate, and the synchronous belt pulley drives the synchronous belt to move, so that the synchronous belt uniformly mixes with the guide rail to rotate;
the reagent box mixing support plate is annular, sleeved on the reagent disk rotating shaft and arranged at the upper part of the mixing track, the lower surface of the reagent disk rotating shaft is provided with mixing rollers, the mixing rollers move on the slope of the mixing track, and the reagent box mixing support plate moves up and down through the change of the upper and lower positions;
the kit mixing backup pad and the upper end swing joint at two guiding axles that the symmetry set up, the guiding axle slides from top to bottom in the guiding axle sleeve, and the spring sets up in the lower part of axle sleeve so that the guiding axle has decurrent drive power.
2. The multi-project combined chemiluminescent instant detection system of claim 1 wherein the kit is a disposable film-sealing kit; the disposable film sealing kit comprises a kit body and a film sealing arranged on the kit body; the box body at least comprises a reagent position and at least one reaction position; the reagent positions comprise a sample position, a mixing position and a pipetting head position; the reaction bits include dense light detection bits.
3. The multi-item joint chemiluminescent instant detection system of claim 1 or 2 wherein the cleaning mechanism is disposed above the kit platform and is fixedly connected to a back plate above the kit platform by a slide rail assembly, the cleaning assembly comprising:
the sliding plate is L-shaped, wherein the vertical side wall is connected with the sliding rail assembly to realize up-and-down sliding, and the horizontal side wall is provided with a plurality of through holes;
the motion module comprises a screw motor and drives the sliding plate to slide up and down;
the liquid sucking and discharging module comprises a plurality of cleaning needles vertically penetrating through holes on the sliding plate, an elastic body is arranged at the upper part of each cleaning needle, a needle baffle is fixedly arranged on the sliding plate, a plurality of through holes matched with the cleaning needles are formed in each needle baffle, the cleaning needles penetrate through the upper parts of the cleaning needles, and the elastic body is stretched and compressed when the cleaning needles slide up and down;
the pushing module comprises a pushing fixing piece which is L-shaped, wherein the vertical side wall is fixedly arranged at the front end of the horizontal side wall of the sliding plate, a pressing block fixing through hole is formed in the horizontal side wall, and the pressing block assembly is elastically connected with the horizontal side wall of the pushing fixing piece.
4. The multi-item joint chemiluminescent instant detection system of claim 3 wherein the briquette assembly comprises a briquette and a support column integrally connected to the upper surface of the briquette, the support column being covered with an elastomer, the upper portion of the support column passing through a through hole in the hold down fixture and being slidably connected.
5. The multi-project integrated chemiluminescent instant detection system of claim 4 wherein the wash needle comprises a liquid suction needle and a liquid injection needle disposed in parallel at the bottom, wherein the liquid suction needle and the upper part of the liquid injection needle are fixedly provided with needle connecting blocks, the liquid injection needle is driven by an eight-way peristaltic pump, and the waste liquid needle is driven by a direct current pump.
6. The multi-item combined chemiluminescence instant detection system of claim 3, wherein a motor fixing block is arranged at the lower part of the screw motor, a photoelectric sensor is arranged on the side wall of the motor fixing block, a photoelectric baffle is arranged on the sliding plate, the photoelectric sensor senses the photoelectric baffle in the process of sliding the sliding plate up and down, and a state signal generated by the photoelectric sensor has level change.
7. The multi-item joint chemiluminescent instant detection system of claim 1 wherein the mixing roller moves on a mixing track ramp to drive the kit to mix at an angle of 0-15 degrees.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010204315.3A CN111323411B (en) | 2020-03-21 | 2020-03-21 | Multi-project combined chemiluminescent instant detection system |
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|---|---|---|---|
| CN202010204315.3A CN111323411B (en) | 2020-03-21 | 2020-03-21 | Multi-project combined chemiluminescent instant detection system |
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| CN111323411B true CN111323411B (en) | 2023-07-04 |
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|---|---|---|---|---|
| CN113970645B (en) * | 2020-06-30 | 2024-10-18 | 苏州液芯生物科技有限公司 | Multi-logic running biological detection equipment, reagent card and detection method thereof |
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