CN111323411A - Multi-project combined chemiluminescence instant detection system - Google Patents
Multi-project combined chemiluminescence instant detection system Download PDFInfo
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- CN111323411A CN111323411A CN202010204315.3A CN202010204315A CN111323411A CN 111323411 A CN111323411 A CN 111323411A CN 202010204315 A CN202010204315 A CN 202010204315A CN 111323411 A CN111323411 A CN 111323411A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a multi-project combined chemiluminescence instant detection system, which comprises: the kit is provided with a plurality of reagent sites required by the test; an instantaneous detection system comprising: the reagent tray bracket is used for bearing the reagent kit, and the center of the lower part of the reagent tray bracket is connected with the reagent tray rotating shaft; the reagent kit comprises a liquid transfer mechanism for transferring liquid, a detection mechanism for collecting luminous information of a reaction system in the reagent kit, and a plurality of reaction positions which are communicated, wherein detection separation strips are arranged in the reaction positions, the bottoms of the detection separation strips are closed, and the upper parts of the detection separation strips are communicated; the instant detection system also comprises a cleaning mechanism and a blending mechanism which are suitable for the reaction position; the blending mechanism is arranged below the reagent kit platform. The invention takes the cup bottom of the reaction cup as a carrier, a plurality of reaction cups are communicated with each other, the antibody or the antigen is marked on the cup bottom, the reagent flows back and forth in each reaction cup through the uniform mixing mechanism after being added, so that the reaction is fully carried out, the detection accuracy is improved, meanwhile, a plurality of items can be simultaneously marked on the cup bottom of the reaction cup, the simultaneous detection of a plurality of items can be carried out under the condition of a small amount of samples, and the time for obtaining the result is shortened.
Description
Technical Field
The invention relates to the technical field of immunodiagnosis detection systems, in particular to a multi-project combined chemiluminescence instant detection system.
Background
At present, immunoassay is an important means of clinical diagnosis, and the mainstream immunoassay method is a chemiluminescence method, has the characteristics of sensitivity, stability and the like, and is widely applied to clinical detection. Although the chemiluminescence has obvious advantages compared with the prior immunoassay method, a magnetic bead coated system is mostly adopted, a magnetic bead cleaning link is adopted in the experiment, the magnetic bead is easy to lose, and the accuracy of the experiment result can be influenced. In addition, the traditional test method can not simultaneously output results in multiple items, even a high-flux instrument can be completed in hours, and the experiments are generally carried out in a specific laboratory environment and are not easy to be used for rapid detection in the field or at the bedside.
Meanwhile, the prior art discloses a chemiluminescent instant detection system, specifically disclosing comprising: the kit is provided with a plurality of reagent sites required by the test; the instant detection system comprises a reagent disk bracket for bearing the reagent kit, wherein the center of the lower part of the reagent disk bracket is connected with a reagent disk rotating shaft; the reagent kit is provided with a liquid transfer mechanism for transferring liquid and a detection mechanism for collecting luminous information of a reaction system in the reagent kit, but the reagent kit is provided with a plurality of reaction positions which are communicated with each other, and adaptive equipment for detecting separation strips is arranged in the reaction positions, and further improvement is needed on the basis of the detection system, so that the uniformity among reaction cups after the test is further improved, the full reaction is obtained, and the accuracy is improved.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention aims to overcome the detection system needing to be coated by magnetic beads in the prior art, the invention takes the cup bottom of a reaction cup as a carrier, a plurality of reaction cups are communicated with each other, antibodies or antigens are marked on the cup bottom, and after a reagent is added, the reagent flows back and forth in each reaction cup through a uniformly mixing mechanism to be fully reacted, so that the magnetic beads are not needed to be taken as the carrier, the washing link of difference caused by loss or unclean washing of the magnetic beads is avoided, the detection accuracy is improved, meanwhile, a plurality of items can be simultaneously marked on the cup bottom of the reaction cup, a plurality of items can be simultaneously detected under the condition of a small amount of samples, and the time for obtaining the result is shortened.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a multi-project combined chemiluminescence instant detection system, comprising:
the kit is provided with a plurality of reagent sites required by the test;
an instantaneous detection system comprising: the reagent tray bracket is used for bearing the reagent kit, and the center of the lower part of the reagent tray bracket is connected with the reagent tray rotating shaft; the reagent box is provided with a liquid transfer mechanism for transferring liquid and a detection mechanism for collecting luminous information of a reaction system in the reagent box;
the kit also comprises a plurality of reaction sites which are communicated, wherein the reaction sites are internally provided with detection separation strips, the bottoms of the detection separation strips are closed, and the upper parts of the detection separation strips are communicated; the instant detection system also comprises a cleaning mechanism and a blending mechanism which are suitable for the reaction position; the blending mechanism is arranged below the reagent kit platform.
Optionally, the kit is a disposable membrane sealing kit; the disposable film sealing kit comprises a box body and a sealing film arranged on the box body; the box body at least comprises a reagent site and at least one reaction site; the reagent position comprises a sample position, a mixing position and a liquid-transferring head position; the reaction site comprises a dense light detection site.
Optionally, wiper mechanism locates kit platform top, through the backplate fixed connection of slide rail set spare and kit platform top, the cleaning assembly includes:
the sliding plate is L-shaped, wherein the vertical side wall is connected with the sliding rail assembly to realize up-and-down sliding, and the horizontal side wall is provided with a plurality of through holes;
the motion module comprises a screw motor and drives the sliding plate to slide up and down;
the liquid suction and discharge module comprises a plurality of cleaning needles vertically penetrating through holes in the sliding plate, elastic bodies are arranged at the upper parts of the cleaning needles, needle blocking pieces are fixedly arranged on the sliding plate, the needle blocking pieces are provided with a plurality of through holes matched with the cleaning needles, the through holes penetrate through the upper parts of the cleaning needles, and when the cleaning needles slide up and down, the elastic bodies can be stretched and compressed;
the downward pressing module comprises a downward pressing fixing piece and is L-shaped, wherein the vertical side wall is fixedly arranged at the front end of the horizontal side wall of the sliding plate, a pressing block fixing through hole is formed in the horizontal side wall, and the pressing block assembly is elastically connected with the horizontal side wall of the downward pressing fixing piece.
Optionally, the briquetting subassembly includes the briquetting and with briquetting upper surface body coupling's support column, the cover is equipped with the elastomer on the support column, the upper portion of support column passes through the through-hole and sliding connection on the lower pressure mounting.
Optionally, the cleaning needle comprises a liquid suction needle and a liquid injection needle which are arranged in parallel at the bottom, a needle connecting block is fixedly arranged on the upper portion of the liquid suction needle and the upper portion of the liquid injection needle, the liquid injection needle is driven by an eight-way peristaltic pump, and the waste liquid needle is driven by a straight-flow pump.
Optionally, a motor fixing block is arranged at the lower part of the screw motor, a photoelectric sensor is arranged on the side wall of the motor fixing block, a photoelectric blocking piece is arranged on the sliding plate, the photoelectric sensor senses the photoelectric blocking piece in the process that the sliding plate slides up and down, and a state signal generated by the photoelectric sensor changes in level.
Optionally, the blending mechanism includes:
the blending bearing is arranged on the rotating shaft of the reagent disk in a close fit manner;
the mixing track is annular and is sleeved on the rotating shaft of the reagent disk and is arranged above the mixing bearing, and the annular side wall of the mixing track is a slope with a curve shape;
the driving assembly comprises a stepping motor, the stepping motor drives a synchronous belt pulley to rotate, the synchronous belt pulley drives a synchronous belt to move, and the synchronous belt mixing guide rail rotates;
the reagent box mixing supporting plate is annular and is sleeved on the reagent plate rotating shaft and arranged on the upper portion of the mixing track, the mixing roller is arranged on the lower surface of the reagent box mixing supporting plate, and the mixing roller moves on the slope of the mixing track and moves up and down through position change.
Optionally, the mixing angle of the reagent kit driven by the movement of the mixing roller on the slope of the mixing track is 0-15 degrees.
Optionally, the reagent kit mixing support plate is movably connected with the upper end parts of two guide shafts symmetrically arranged, the guide shafts slide up and down in the guide shaft sleeves, and the springs are arranged at the lower parts of the shaft sleeves to enable the guide shafts to have downward driving force.
Optionally, the reagent box is placed on a reagent box base, the reagent box base is arranged on a reagent plate support, an insulating film, a heating plate and a damping sheet are sequentially arranged at the bottom of the reagent plate support from top to bottom, a reagent box roller is arranged in the middle of the lower portion of the damping sheet, and a torsion spring assembly is arranged at the outer edge of the lower portion of the damping sheet.
The technical scheme of the invention has the following advantages:
the disposable membrane sealing kit with a plurality of reagent positions required by the test and the multi-project combined chemiluminescence instant detection system provided by the embodiment of the invention comprise a liquid transfer mechanism for transferring liquid, a uniform mixing mechanism for fully reacting reagents in a plurality of reaction cups, a detection mechanism for collecting luminescence information of a reaction system in the reagent cup and a cleaning mechanism for cleaning; the reaction cups are communicated, and after the reagent is added, the reagent flows back and forth in each reaction cup through the uniformly mixing mechanism, so that the reagent can be fully reacted. The structure avoids using magnetic beads as carriers for adsorbing and combining various molecules, and simultaneously, the kit and the pipetting head are made of disposable materials, so that test errors and cross contamination of reagents and strains are reduced to a great extent, and the accuracy of the test is improved.
According to the multi-project combined chemiluminescence instant detection system provided by the embodiment of the invention, the disposable liquid transfer head and the kit are used as carriers for liquid transfer and storage, and the liquid transfer head can be recycled after a test, so that chemical pollution is reduced; a plurality of functional areas are arranged in the kit, one sample corresponds to one test box, and no additional consumable is added; the whole instrument has compact structure, is small and portable, and is suitable for field detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the platform structure of the kit of the present invention;
FIG. 3 is a schematic view of the structure of the pipetting mechanism of the invention;
FIG. 4 is a schematic structural diagram of the kit of the present invention;
FIG. 5 is a schematic diagram of a reaction site;
FIG. 6 is a schematic view of the structure of the test strip;
FIG. 7 is a schematic view of the cleaning mechanism of the present invention;
FIG. 8 is a schematic view of the cleaning mechanism assembly of the present invention;
FIG. 9 is a schematic structural view of the combination needle;
FIG. 10 is a schematic diagram of a blending configuration of the present invention;
FIG. 11 is a schematic view of the mixing rail of FIG. 10;
fig. 12 is a schematic diagram of the cartridge tray assembly.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
The instant detection system for multi-project combined chemiluminescence provided by the embodiment comprises a disposable membrane sealing kit and a multi-project combined instant detection system for chemiluminescence. Referring to fig. 1, the chemiluminescence instant detection system comprises a kit platform 1 for carrying a kit, a liquid transfer mechanism 2 for transferring liquid, a cleaning mechanism 5 for cleaning, a detection mechanism 4 for collecting luminescence information of a reaction system in the kit, and a blending mechanism.
Specifically, referring to fig. 2, the reagent kit platform 1 is a rotatable disk structure, the reagent kit platform 1 includes a reagent kit bracket 14, the reagent kit bracket 14 can be matched with a reagent kit, so that the reagent kit is placed on the reagent kit bracket 14, the platform of this embodiment can carry and transfer twelve reagent kits 11, and the reagent kits 11 can be freely taken from and placed on the reagent kit platform; the reagent kit platform 1 is also provided with a temperature control module for providing incubation reaction temperature and a reagent kit mixing mechanism, the reagent kit platform motion module can control the reagent kit 11 to rotate on the disc, and the reagent kit platform motion module is matched with the liquid transferring mechanism 2 to finish liquid transferring and mixing operations and matched with the detection mechanism 4 to finish detection of a reagent kit reaction system; referring to fig. 3, the pipetting mechanism 2 at least comprises a pipetting assembly, a moving platform, a slide rail control module 21 for controlling the pipetting assembly to move linearly horizontally or vertically, and a screw rod moving module 22; the motion platform can do two-dimensional motion at least in a plane vertical to the reagent box platform and also can be composed of a three-dimensional motion mechanism. The liquid-transfering component can be fixed on the moving platform, and can make two-dimensional or three-dimensional movement along with the moving platform, and said liquid-transfering component includes piston mechanism 3 combined with its interior and plunger head elastically connected with lower end of piston mechanism 3, and its bottom end can be connected with disposable liquid-transfering head. Piston mechanism 3 comprises piston rod and cavity, and the outside guide of piston, piston rod can be driven the up-and-down motion by the motor, and inflation or compressed air move the liquid head that the liquid subassembly is connected in the cooperation, can accomplish the operation of moving the liquid. Specifically, move liquid mechanism 2 and constitute by moving liquid subassembly, lead screw motion module 22 and slide rail motion module 21, and piston mechanism 3 is connected with lead screw motion module 22, and piston mechanism 3 can descend to the liquid removal head 116 on the kit under the control of lead screw motion module 22, closely cooperates through the top of rigid connector 32 and liquid removal head, and rigid connector 32 can guarantee the gas tightness of structure. The pipetting head is driven by the screw rod motion module 22 to perform linear motion in the vertical direction. The screw rod motion module 22 is connected with the slide rail motion module 21, and the screw rod motion module 22 can move in the horizontal direction under the control of the slide rail motion module 21. The piston mechanism 3 is composed of a piston and a piston motion control module 31, one end of the piston is inserted into the hard connector 32, and is connected with the hard connector 32 through a sealing ring positioned on the top of the hard connector 32 to keep air tightness. The other end of the piston is connected with a piston motion control module 31, and the piston motion control module controls the linear motion of the piston in the vertical direction. In an embodiment, the piston motion control module 31 is coupled to the control piston. When acquiring the liquid transfer head, the reagent kit platform 1 drives the reagent kit 11 to move, the rigid connector 32 is moved to the vertical upper side of the liquid transfer head by the slide rail motion module 21 in the liquid transfer mechanism 2, then the rigid connector 32 is descended to the liquid transfer head inserted into the reagent kit 11 through the screw rod motion module 22 in the liquid transfer mechanism 2, the screw rod motion module 22 in the liquid transfer mechanism 2 is ascended to be separated from the reagent kit 11, and the liquid transfer head is acquired. When liquid is transferred, the reagent kit platform 1 drives the reagent kit 11 to move to a position right below the liquid transferring head, the liquid transferring head moves to a hole position of the reagent kit 11 needing to transfer liquid through the slide rail motion module 21 in the liquid transferring mechanism 2, the liquid transferring head enters liquid in a corresponding hole position of the reagent kit 11 needing to transfer liquid under the driving of the screw rod motion module 22 in the liquid transferring mechanism 2, at the moment, the piston 3 moves upwards under the control of the piston motion control module 31 to enable the liquid to enter the liquid transferring head, then the screw rod motion module 22 in the liquid transferring mechanism 2 moves upwards to drive the liquid transferring head 11 to leave the reagent kit 11, the slide rail motion module 21 in the liquid transferring mechanism 2 moves to a corresponding hole position of the reagent kit 11 needing to bear liquid, the liquid transferring head 12 enters a corresponding position of the reagent kit 11 bearing liquid under the driving of the screw rod motion module 22 in the liquid transferring mechanism 2, and the piston mechanism 3 moves downwards under the control of the, and discharging the liquid in the liquid transferring head to a target position to finish the liquid transferring.
The disposable membrane sealing kit is shown in figure 4, and consists of a kit 11 and a detection cup; the kit 11 comprises a plurality of functional separation sites, which includes a sample site 112, a mixing site 110, 111, a reagent site 113, 114, a dense light detection site (reaction site) 115 and a TIP site 116 in this embodiment, wherein the dense light detection site (reaction site) 115 contains a detection separation strip, the reagent sites 113 and 114 have a plurality of sites, which can be selected and functionally expanded according to the application, the sample site includes but is not limited to a sample loading site and a sample sampling site, the sample loading site is communicated with the sample sampling site, the sample site has a filter membrane therein, which can filter most of impurities in the whole blood in the sample loading site, so that the serum sample enters the sample sampling site through the filter membrane; the box body can be filled with a defined reaction reagent and a reaction cup in advance and then is sealed with a top film; the disposable membrane sealing kit used in the test is a consumable and is replaced along with the completion of the test. The dense light detection position is composed of a plurality of reaction positions which are communicated with each other, an 8-hole detection separation strip is arranged in each reaction position, 8 detection holes are formed in each detection strip, the bottoms of the detection holes are closed, the lower portions of the detection holes are mutually independent, and the upper portions of the detection holes are communicated through a connecting channel.
Specifically, referring to fig. 8, the lead screw motor is fixed on the motor fixing part 51 511, the guide rail sliding block 526 can slide on the guide rail 527, the needle fixing part 524 is fixed on the guide rail sliding block 526, so that the needle fixing part 524 can move up and down, the lead screw nut 525 is fixed on the needle fixing part 524, the lead screw of the lead screw motor 51 penetrates through the lead screw nut 525, so that the lead screw motor 51 drives the needle fixing part 524 to move when moving, the photoelectric blocking piece 529 is fixed on the needle fixing part 524, the photoelectric sensor 528 is fixed on the motor fixing part 511, and when the needle fixing part 524 moves, the photoelectric blocking piece 529 triggers the photoelectric sensor 528 to accurately position signals. The cleaning needle 523 is shown in fig. 9 and comprises a liquid suction needle, a liquid injection needle and a needle connecting block, the total number of the cleaning needles is 8, 8 liquid injection needles and 8 waste liquid suction needles are provided, the liquid injection needles are driven by an 8-way peristaltic pump, cleaning liquid with any volume can be added at the same time, the waste liquid needles are driven by a direct-current pump, the cleaning needles are rapid and efficient, 8 waste liquids adopt an independent self-adaptive structure, the problem that the waste liquid cannot be sucked completely due to uneven reagent kit can be avoided, the cleaning needle 523 is installed in a through hole of the needle fixing member 524, the cleaning needle 523 can slide up and down in the through hole of the needle fixing member 524, a spring 522 is installed on the cleaning needle 523, a needle blocking piece 521 is fixed on the needle fixing member 524, the spring 522 is pressed, and self-adaptation. The downward pressing module 53 comprises a fixing part for fixing the division bar pressing block 531 on the needle fixing part 524, the pressing block 532, the pressing block 533 and the pressing block 534 are fixed on the fixing part for fixing the division bar pressing block 531, the pressing block 534 and the pressing block 533 are composed of springs and 2 support columns, and through holes of the support columns on the fixing part for fixing the division bar pressing block 531 can move up and down, so that self-adaptation of an instrument during division bar pressing can be realized. 8 notes liquid needles and 8 waste liquid needles of inhaling, annotate the liquid needle and drive through an 8 peristaltic pump, can add arbitrary volume washing liquid simultaneously, the waste liquid needle is through a straight-flow pump drive, and is high-efficient fast, and 8 waste liquids adopt independent self-adaptation structure, can have to avoid because the kit is uneven leads to the waste liquid to inhale unclean problem.
As shown in fig. 10, the blending mechanism is characterized in that a reagent disk platform 1 is fixed on a reagent disk rotating shaft 69, a reagent disk rotating bearing 68 is tightly fitted below the reagent disk rotating shaft, a rotating shaft sleeve 67 is installed on a reagent disk mounting plate 64, and a locking nut 65 is fixed on the reagent disk mounting plate 64; a blending bearing 66 is tightly installed above a reagent disk rotating shaft 69 in a matching mode, a blending guide rail 624 is installed on the outer side of the blending bearing 66, a synchronous pulley 626 is installed on a stepping motor 627, the stepping motor 627 moves to drive the synchronous pulley 626 to move, the synchronous pulley 626 drives a synchronous belt 625 to move, the synchronous belt 625 drives the blending guide rail 624 to move, a slope is arranged on a blending track 624, as shown in fig. 11 specifically, the movement position of a blending roller 623 on the slope of the track is changed, so that the blending support of a reagent kit is moved up and down, the blending support of the reagent kit is fixed on two guide shafts 651, the guide shafts 651 can slide up and down in a guide shaft sleeve 652, and the guide shafts 651; the kit is placed on the kit collet, and kit gyro wheel 621 is fixed in the inboard of kit collet bottom, and kit gyro wheel 621 can move on kit mixing backup pad 622, and when the up-and-down motion was supported in the kit mixing, kit gyro wheel 621 also up-and-down motion, and the up-and-down motion is realized in the kit follow-up, reaches the mixing purpose.
Referring to fig. 12, the reagent box is placed on a reagent box bottom support 71, the reagent box bottom support 71 is arranged on a reagent plate support, an insulating film 72, a heating plate 74 and a damping sheet 73 are sequentially arranged at the bottom of the reagent plate support from top to bottom, a reagent box roller is arranged in the middle of the lower portion of the damping sheet 73, a torsion spring assembly 75 is arranged at the outer edge of the lower portion of the damping sheet, and the torsion spring is connected with a torsion spring fixing block through a torsion spring shaft.
The chemiluminescence detection system also comprises a liquid crystal display screen and a printer, wherein the liquid crystal display screen can be used for carrying out experiment setting and result display, and the printer can be used for carrying out result printing.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A multi-project combined chemiluminescence instant detection system, comprising:
the kit is provided with a plurality of reagent sites required by the test;
an instantaneous detection system comprising: the reagent tray bracket is used for bearing the reagent kit, and the center of the lower part of the reagent tray bracket is connected with the reagent tray rotating shaft; has a liquid transfer mechanism for transferring liquid and a detection mechanism for collecting luminous information of a reaction system in the reagent box,
the kit also comprises a plurality of reaction sites which are communicated, wherein the reaction sites are internally provided with detection separation strips, the bottoms of the detection separation strips are closed, and the upper parts of the detection separation strips are communicated; the instant detection system also comprises a cleaning mechanism and a blending mechanism which are suitable for the reaction position; the blending mechanism is arranged below the reagent kit platform.
2. The multi-item combined chemiluminescent real-time detection system of claim 1 wherein the kit is a disposable membrane-sealed kit; the disposable film sealing kit comprises a box body and a sealing film arranged on the box body; the box body at least comprises a reagent site and at least one reaction site; the reagent position comprises a sample position, a mixing position and a liquid-transferring head position; the reaction site comprises a dense light detection site.
3. The multi-project combined chemiluminescence instant detection system according to claim 1 or 2, wherein the cleaning mechanism is arranged above the reagent kit platform and is fixedly connected with a back plate above the reagent kit platform through a slide rail assembly, and the cleaning assembly comprises:
the sliding plate is L-shaped, wherein the vertical side wall is connected with the sliding rail assembly to realize up-and-down sliding, and the horizontal side wall is provided with a plurality of through holes;
the motion module comprises a screw motor and drives the sliding plate to slide up and down;
the liquid suction and discharge module comprises a plurality of cleaning needles vertically penetrating through holes in the sliding plate, elastic bodies are arranged at the upper parts of the cleaning needles, needle blocking pieces are fixedly arranged on the sliding plate, the needle blocking pieces are provided with a plurality of through holes matched with the cleaning needles, the through holes penetrate through the upper parts of the cleaning needles, and when the cleaning needles slide up and down, the elastic bodies can be stretched and compressed;
the downward pressing module comprises a downward pressing fixing piece and is L-shaped, wherein the vertical side wall is fixedly arranged at the front end of the horizontal side wall of the sliding plate, a pressing block fixing through hole is formed in the horizontal side wall, and the pressing block assembly is elastically connected with the horizontal side wall of the downward pressing fixing piece.
4. The multi-item combined chemiluminescence instant detection system according to claim 3, wherein the briquetting component comprises a briquetting and a support column integrally connected with the upper surface of the briquetting, an elastomer is sleeved on the support column, and the upper part of the support column passes through the through hole on the downward pressing fixing piece and is connected in a sliding manner.
5. The multi-project combined chemiluminescence instant detection system according to any one of claims 1 to 4, wherein the cleaning needle comprises a liquid suction needle and a liquid injection needle which are arranged in parallel at the bottom, a needle connecting block is fixedly arranged at the upper parts of the liquid suction needle and the liquid injection needle, the liquid injection needle is driven by an eight-way peristaltic pump, and the waste liquid needle is driven by a straight flow pump.
6. The system of claim 3, wherein a motor fixing block is disposed at a lower portion of the screw motor, a photo sensor is disposed on a sidewall of the motor fixing block, a photo stop is disposed on the sliding plate, the photo sensor senses the photo stop when the sliding plate slides up and down, and a status signal generated by the photo sensor changes in level.
7. The multi-project combined chemiluminescence instant detection system of claim 1, wherein the blending mechanism comprises:
the blending bearing is arranged on the rotating shaft of the reagent disk in a close fit manner;
the mixing track is annular and is sleeved on the rotating shaft of the reagent disk and is arranged above the mixing bearing, and the annular side wall of the mixing track is a slope with a curve shape;
the driving assembly comprises a stepping motor, the stepping motor drives a synchronous belt pulley to rotate, the synchronous belt pulley drives a synchronous belt to move, and the synchronous belt mixing guide rail rotates;
the reagent box mixing supporting plate is annular and is sleeved on the reagent plate rotating shaft and arranged on the upper portion of the mixing track, the mixing roller is arranged on the lower surface of the reagent box mixing supporting plate, and the mixing roller moves on the slope of the mixing track and moves up and down through position change.
8. The multi-item combined chemiluminescence instant detection system according to claim 7, wherein the blending roller moves on the blending track slope to drive the blending angle of the kit to be 0-15 degrees.
9. The multi-item combined chemiluminescence instant detection system according to claim 7, wherein the reagent kit blending support plate is movably connected with the upper end parts of two symmetrically arranged guide shafts, the guide shafts slide up and down in the guide shaft sleeves, and the springs are arranged at the lower parts of the shaft sleeves to enable the guide shafts to have downward driving force.
10. The system of claim 7, wherein the reagent kit is placed on a reagent kit holder, the reagent kit holder is disposed on a reagent tray support, the bottom of the reagent tray support is sequentially provided with an insulating film, a heating plate and a damping sheet from top to bottom, a reagent kit roller is disposed at a position near the middle of the lower portion of the damping sheet, and a torsion spring assembly is disposed at a position near the outer edge of the lower portion of the damping sheet.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010204315.3A CN111323411B (en) | 2020-03-21 | 2020-03-21 | Multi-project combined chemiluminescent instant detection system |
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|---|---|---|---|
| CN202010204315.3A CN111323411B (en) | 2020-03-21 | 2020-03-21 | Multi-project combined chemiluminescent instant detection system |
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| CN111323411A true CN111323411A (en) | 2020-06-23 |
| CN111323411B CN111323411B (en) | 2023-07-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112985968A (en) * | 2021-04-19 | 2021-06-18 | 双胞胎（集团）股份有限公司 | Detection equipment for water-soluble compound feed |
| WO2022226746A1 (en) * | 2021-04-26 | 2022-11-03 | 深圳市帝迈生物技术有限公司 | Poct blood cell analyzer and using method therefor |
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