CN111999512A

CN111999512A - Chemiluminescence immunity analyzer

Info

Publication number: CN111999512A
Application number: CN201910391246.9A
Authority: CN
Inventors: 许行尚; 杰弗瑞·陈; 应家树; 赵大强; 张前军
Original assignee: Nanjing Lanyu Biological Technology Co Ltd
Current assignee: Nanjing Lanyu Biological Technology Co Ltd
Priority date: 2019-05-11
Filing date: 2019-05-11
Publication date: 2020-11-27

Abstract

The invention discloses a chemiluminescence immunoassay analyzer, which comprises a base, wherein the base is provided with: the sample injection module comprises a quintuplet cup for loading a reagent; the incubation magnetic separation cleaning module comprises an incubation reaction disc device, a magnetic separation device and a cleaning device; the clamping jaw and sampling needle module comprises a motion component part, a clamping jaw, a sampling needle and a sampling needle cleaning device; the clamping jaw is used for assembling a sampling needle in a combined mode, and the sampling needle is used for transferring liquid in the quintuplet cup; the sampling needle cleaning device is used for cleaning the sampling needle; and the measuring module is used for carrying out chemiluminescence detection on the reaction cup. All the components are integrated on the base to form a whole set of equipment, the structure is integrated, the streamlined operation is realized, and the test time is short; all reagents are integrated in a quintuplet cup, so that the purpose of sampling and testing can be realized, the use is convenient, the clinical testing time is saved, the quintuplet cup avoids the pollution possibly caused by the repeated cleaning and use of the reagents and the sample tube or the test card box, and the detection accuracy is improved.

Description

Chemiluminescence immunity analyzer

Technical Field

The invention belongs to the technical field of medical equipment, and particularly relates to a chemiluminescence immunoassay analyzer for a chemiluminescence diagnosis technology.

Background

The mainstream chemiluminescence product in the market needs to adopt serum or plasma as a detection sample, can not support direct detection of a whole blood sample, has long first sample report time and long total diagnosis time, hardly meets the requirement of detection turnover time, has long total waiting time particularly, can aggravate the pain of patients, easily causes the conflict emotion of family members or patients, and is not beneficial to the treatment of subsequent patients.

Chinese patent document CN106918715A discloses a chemiluminescent diagnostic device comprising: a base having a mounting surface; the sampling needle module comprises a sampling mechanism which is rotatably arranged relative to the base and a driving mechanism which is arranged on the base and is used for driving the sampling mechanism to rotate and lift; a magnetic bead reagent pre-incubation module comprising a pre-incubation tray and pre-incubation heating means for heating the pre-incubation tray, the pre-incubation heating means being disposed at the bottom of the pre-incubation tray; the pre-incubation disc is arranged around the rotating axis of the sampling mechanism and is positioned in a sampling area of the sampling mechanism; the incubation cleaning module comprises an incubation tray, an incubation heating device for heating the incubation tray, a magnetic separation component and a cleaning station component, wherein the incubation heating device is arranged at the bottom of the incubation tray, the magnetic separation component is sleeved at the outer edge of the incubation tray, the cleaning station component comprises a driving component and a cleaning needle fixed on the driving component, the cleaning needle is arranged above the incubation tray and can be matched with an inner cavity of an incubation reaction cup on the incubation tray in a working state; and the data analysis module comprises an industrial personal computer and a touch display screen for data analysis.

However, the nuclear chemiluminescence diagnostic apparatus in the above technical solution is not ideal in the specific use process.

Disclosure of Invention

The invention aims to provide a chemiluminescence immunoassay analyzer which can be used for rapidly analyzing samples and has short chemiluminescence diagnosis time.

In order to solve the technical problems, the technical scheme adopted by the invention is that the chemiluminescence immunoassay analyzer comprises a base, wherein the base is provided with:

the sample introduction module comprises a quintuplet cup for loading a reagent;

the incubation magnetic separation cleaning module comprises an incubation reaction disc device, a magnetic separation device and a cleaning device; the incubation reaction plate device is used for incubating the reaction cups in the quintuplet cup and keeping constant-temperature reaction; the magnetic separation device is used for adsorbing magnetic beads in the reaction cup; the cleaning device is used for cleaning the magnetic beads in the reaction cup;

the clamping jaw and sampling needle module comprises a motion component part, a clamping jaw, a sampling needle and a sampling needle cleaning device; the sampling device comprises a sampling needle, a clamping jaw, a moving component part, a clamping jaw and a power supply part, wherein the moving component part is composed of an up-and-down moving component and a horizontal moving component, the horizontal moving component is used for driving the sampling needle and the clamping jaw to move horizontally, and the up-and-down moving component is used for enabling the sampling needle and the clamping jaw to move up and down; the clamping jaw is used for transferring a sample reaction cup and a labeled antibody cup in a quintuplet cup, and the sampling needle is used for transferring liquid in the quintuplet cup; the sampling needle cleaning device is used for cleaning the sampling needle;

and the measuring module is used for carrying out chemiluminescence detection on the reaction cup.

By adopting the technical scheme, all the components are integrated on the base to form the whole set of equipment, the structure is integrated, the streamlined operation is realized, the test time is short, and the using effect is good; the quintuplet cup is adopted in the sample introduction module, all reagents required by detection are integrated into the quintuplet cup, the purpose of sampling and detecting is achieved, the use is convenient, the clinical detection time is saved, meanwhile, the quintuplet cup is a disposable consumable in the detection process, the pollution possibly caused by repeated cleaning and use of the reagents and the sample tube or the test card box can be avoided, and the detection accuracy is improved; the incubation magnetic separation cleaning module has the effects of incubation, magnetic separation and cleaning, incubates the reaction cup, keeps constant temperature reaction, shortens incubation time, can shorten chemiluminescence diagnosis time, cleans magnetic beads in the reaction cup, and simultaneously the magnetic separation device realizes that the magnetic beads in the reaction cup are adsorbed by a magnet; the clamping jaw and sampling needle module realizes the combined assembly of the clamping jaw and the sampling needle, and has compact structure; the measuring module is used for carrying out chemiluminescence detection on the reaction cup, and the whole equipment is reasonable in overall layout and compact in structure.

Preferably, the sample introduction module further comprises a sample introduction device body, a ball plunger and a driving device, the ball plunger is connected to the sample introduction device body, and the driving device drives and controls the quintuplet cup to move; the ball plunger is used for limiting the quintuplet cup.

By adopting the technical scheme, the quintuplet cup is driven to move by the driving device, meanwhile, the quintuplet cup is limited by the ball plunger in the moving process, and the ball plunger is clamped with the clamping groove of the quintuplet cup, namely, the quintuplet cup is in place.

Preferably, the sample introduction module further comprises a hand dialing device, the hand dialing device comprises a first hand dialing part, a second hand dialing part and a connecting rod, the first hand dialing part and the second hand dialing part are respectively arranged at two ends of the connecting rod, and a hand dialing rotating shaft is arranged at one side of the first hand dialing part; the connecting rod is connected with a sample injection spring below the first shifting handle through a spring fixing block, one end of the sample injection spring is in press contact with the first shifting handle, and an optocoupler limiting sheet is connected below the other end of the sample injection spring; the driving device drives the quintuplet cup to move through the hand poking device.

The driving device drives the quintuplet cup to move by utilizing the first shifting hand and the second shifting hand through the arrangement of the hand pulling device, and meanwhile, the first shifting hand and the second shifting hand have a limiting effect on the quintuplet cup, so that the movement of the quintuplet cup and the position of the quintuplet cup after the movement are better controlled; the position of the hand dialing device can be prompted by arranging the optical coupling limiting piece.

Preferably, the device for the incubation reaction disc comprises a bottom plate, wherein an incubation bracket is arranged on the bottom plate, the incubation disc is positioned on the incubation bracket, and a heating sheet and a temperature sensor are arranged on the back surface of the incubation disc; an incubation disc two-stage transmission component part is arranged below the incubation disc and consists of a two-stage transmission motor, a first-stage transmission component and a second-stage transmission component; the two-stage transmission motor drives a first-stage driving wheel in the first-stage transmission assembly, the first-stage driving wheel drives a first-stage driven wheel, the first-stage driven wheel and a second-stage driving wheel rotate coaxially, the second-stage driving wheel drives a second-stage driven wheel, the second-stage driven wheel and an incubation disc rotating shaft rotate coaxially, and the incubation disc rotating shaft drives the incubation disc to rotate; the radiuses of the first-stage driven wheel and the second-stage driven wheel are larger than those of the first-stage driving wheel and the second-stage driving wheel.

The incubation reaction plate device adopts the structure, so that the incubation of the reaction cup can be better realized, and the constant-temperature reaction is kept; the two-stage transmission is adopted to reduce the running pressure of the motor and control the rotation of the incubation disc, and specifically, the two-stage transmission motor controls the rotation of a first-stage driving wheel, the first-stage driving wheel drives a first-stage driven wheel, the first-stage driven wheel drives a second-stage driving wheel, the second-stage driving wheel drives a second-stage driven wheel to rotate, a rotating shaft of the second-stage driven wheel is fixedly connected with the center of the incubation disc, then the incubation disc rotates, the damage of the motor is reduced by adopting the second-stage; the bottom surface of the incubation disc is provided with a circular heating plate and a temperature sensor which are integrally arranged and adhered to the bottom surface of the incubation disc for controlling the temperature and keeping the reaction in a constant temperature environment.

Preferably, the incubation disc is provided with an outer ring reaction cup position and an inner ring reaction cup position which are both provided with a plurality of cup holes for placing reaction cups; the two-step chemiluminescence reaction time is reduced, and no water pan is used.

Preferably, the incubation support has a receiving cavity; the two-stage transmission motor is positioned in the accommodating cavity, and a circuit board, a relay and a temperature controller are also arranged in the accommodating cavity; compact structure, reasonable layout and small occupied space.

Preferably, the magnetic separation device comprises an arc-shaped push block and a magnet frame; the magnet frame consists of a plurality of magnet seats, each magnet seat consists of a magnet fixing sleeve and an internal magnet assembly, and the magnet fixing sleeves are fixed on the magnet frame through bolts; the internal magnet assembly comprises a spring and a magnet at the end; the arc-shaped pushing block is fixedly connected with a linear bearing support.

The magnetic separation device adopting the structure utilizes the motor to control the up-and-down movement and horizontal pushing of the magnet seat, and has simple structure and low cost.

Preferably, a vertical plate is arranged below the arc-shaped push block, the vertical plate is connected with a transverse plate fixedly provided with a screw nut, the vertical plate drives a screw rod by a screw rod motor, and the screw nut is sleeved on the screw rod to enable the vertical plate to move up and down; the outer edge of the arc-shaped push block is provided with a small vertical push block, the small vertical push block is provided with an inclined plane structure, and the inclined plane structure generates thrust on the spring in the magnet seat to enable the magnet at the tail end to contact the reaction cup.

The lead screw motor drives the structure motion of diaphragm and riser rigid coupling, upward movement drives the arc ejector pad, arc ejector pad and linear bearing pillar rigid coupling, the linear bearing pillar motion, the arc ejector pad is also upward movement, the arc ejector pad is outer along having vertical little ejector pad, vertical little ejector pad has the inclined plane structure, the inclined plane produces the thrust to the reaction cup direction to the spring in the magnet seat, thrust makes magnet contact reaction cup side bottom, the magnetic bead is adsorbed by magnetic force, reverse realization breaks away from promptly.

Preferably, the cleaning device comprises a cleaning bracket, a cleaning needle connecting bracket is arranged on the cleaning bracket, and a cleaning needle assembly is arranged on the cleaning needle connecting bracket; the cleaning device further comprises a cleaning device motor, and the cleaning device motor is fixedly connected with a fixing block; the cleaning needle is characterized in that the cleaning support is also provided with a limiting nut, the fixed block is connected with a connecting rod, the connecting rod is fixedly connected with a connecting slide block, and the connecting slide block is connected with the cleaning needle connecting support; the connecting slide block moves up and down along the guide rail on the cleaning bracket.

Preferably, the side part of the cleaning needle connecting bracket is provided with an optical coupler and a baffle plate; the cleaning needle assembly is provided with a plurality of cleaning needles which are connected with spring pieces; the cleaning needle consists of a liquid inlet needle and a liquid discharge needle, wherein the liquid inlet needle is shorter than the liquid discharge needle, and the needle head is bent towards the liquid discharge needle.

And cleaning the magnetic beads in the reaction cup, discharging waste liquid in the reaction cup by using the cleaning needle, and simultaneously injecting cleaning liquid, so that the purpose of cleaning the magnetic beads is repeatedly achieved.

The cleaning device is characterized in that a motor of the cleaning device is started, a fixed block is fixedly connected with a rotating shaft of the motor, a limiting nut ensures that the fixed block can only rotate anticlockwise, the lower part of a connecting rod is fixedly connected with the fixed block and rotates anticlockwise, the upper part of the connecting rod is fixedly connected with a connecting slide block, the connecting slide block is simultaneously connected with a cleaning needle connecting support, and the slide block moves on a guide rail, so that the upper part of the connecting rod drives the cleaning needle connecting support to move downwards along the guide rail, and a cleaning needle assembly is fixedly connected to the cleaning needle; similarly, when wasing needle subassembly upward movement, the belt cleaning device motor drives the fixed block clockwise rotation, and stall when moving to limit nut, simultaneously, washing needle linking bridge lateral part is provided with opto-coupler and separation blade, when wasing needle upward movement, when the separation blade reachs the opto-coupler position, opto-coupler feedback signal makes the motor stall.

The cleaning needle is connected with the spring leaf, and the damage is caused to the contact reaction bottom of cup when preventing that the cleaning needle from unexpected whereabouts, and the spring leaf can provide the buffering, protects the cleaning needle. The cleaning needle consists of a liquid inlet needle and a liquid discharge needle, the liquid inlet needle is shorter than the liquid discharge needle, and the needle head is bent to the liquid discharge needle, so that the outer wall of the liquid discharge needle can be cleaned simultaneously when liquid is fed.

Preferably, the horizontal movement assembly comprises a sampling needle horizontal movement assembly and a clamping jaw horizontal movement assembly, wherein the sampling needle horizontal movement assembly is composed of a sampling needle horizontal movement motor and a sampling needle spline shaft, and the clamping jaw horizontal movement assembly is composed of a clamping jaw horizontal movement motor and a clamping jaw spline shaft; the sampling needle spline shaft penetrates through the clamping jaw spline shaft, so that the sampling needle and the clamping jaw are assembled in a combined manner; the sampling needle horizontal movement motor drives a sampling needle spline shaft, and the sampling needle spline shaft is connected with a needle arm and then drives the sampling needle to move horizontally; the clamping jaw horizontal movement motor drives the clamping jaw to move horizontally by driving the clamping jaw spline shaft; the up-and-down movement assembly comprises a sampling needle up-and-down movement assembly and a clamping jaw up-and-down movement assembly, wherein the sampling needle up-and-down movement assembly comprises a sampling needle up-and-down movement motor, a first fixing block and a first guide shaft sliding block; a damping encoder is arranged on the sampling needle up-and-down movement motor; the clamping jaw up-and-down movement assembly comprises a clamping jaw up-and-down movement motor, a second fixing block and a second guide shaft sliding block, the clamping jaw up-and-down movement motor enables the second guide shaft sliding block to move up and down along the guide shaft through the second fixing block, and the second guide shaft sliding block is fixedly connected with the clamping jaw spline shaft, so that the clamping jaw can move up and down.

Preferably, the clamping jaw comprises the clamping jaw spline shaft, a clamping jaw arm connecting block, a chuck mounting connecting piece, a clamping jaw mounting plate, a chuck, a torque measuring device and a mirror chuck; the chuck is provided with a groove, and the mirror chuck is matched with the chuck to grab the reaction cup; the mirror chuck and the chuck are both connected to the chuck mounting connecting piece; the chuck mounting connecting piece is connected with the clamping jaw mounting plate, and a chuck motor and the torque measuring device are arranged on the clamping jaw mounting plate; the clamping jaw arm connecting block with the clamping jaw mounting panel is connected, just the clamping jaw integral key shaft is located on the clamping jaw arm connecting block.

The horizontal movement and the up-and-down movement of the sampling needle and the clamping jaw are respectively controlled by different motors, the horizontal movement of the sampling needle is controlled by a sampling needle horizontal movement motor, a rotating shaft is connected with the motor, a driving wheel is connected with the rotating shaft, a driven wheel is driven to rotate through a belt, a sampling needle spline shaft is connected with the driven wheel, the sampling needle spline shaft is connected with a needle arm, and then the sampling needle is driven to move horizontally; when the sampling needle moves up and down, the sampling needle up-and-down movement motor is started to drive the driving wheel to rotate, the driving wheel drives the driven wheel through the belt, the belt is fixedly connected with the first fixing block, the first fixing block is fixedly connected with the first guide shaft sliding block, so that the first guide shaft sliding block moves up and down along the guide shaft, and the first guide shaft sliding block is fixedly connected with the spline shaft of the sampling needle, so that the sampling needle can move up and down; the damping encoder is arranged on the sampling needle up-and-down movement motor, so that the restriction force is exerted, the sampling needle is prevented from being damaged by accidental falling, and the control is accurate.

In a similar way, the horizontal movement and the up-and-down movement of the clamping jaw are respectively composed of a corresponding motor and a matched movement component.

Preferably, the sampling needle is provided with a needle arm, the needle arm is provided with a needle arm upper cover, and the needle arm upper cover and the needle arm are assembled to form a sampling arm cavity; an optical coupler, a liquid level sensor, an induction baffle and a spring baffle are arranged in the sampling arm cavity; a spring, an induction sheet and a sliding sleeve are sequentially arranged below the spring retaining sheet; and a sleeve, an insulating fixing ring and an inner needle tube are sequentially arranged below the sliding sleeve.

When the sampling needle touches the bottom of the reaction cup, the induction sheet and the induction separation blade are integrated, the linkage spring is compressed to provide buffering, the change of the induction separation blade is sensed by the optical coupler, a signal is transmitted to the CPU, and an instrument reports errors and corrects; and a liquid level sensor is arranged in the sampling arm cavity and used for sensing the accuracy of liquid suction and liquid adding quantity of the sampling needle.

Preferably, the sampling needle cleaning device comprises a sampling needle cleaning bracket and a cleaning pool; the cleaning pool is provided with a side liquid inlet and a lower liquid outlet.

When wasing the sampling needle, the sampling needle is located washs pond top in the pond, and the inside washing of sampling needle is passed through the inside washing of needle by feed liquor system, and the waste liquid gets into and washs pond in the pond, and the needle outside gets into upwards washing needle outer wall by side feed inlet, and the inside and outside washing is gone on simultaneously, washs the back waste liquid and is discharged through flowing back system by wasing pond lower part leakage fluid dram.

Preferably, the measuring module comprises a detection inner chamber, the reaction cup is placed into the detection inner chamber by the clamping jaw, and the detection inner chamber is controlled by a motor of the measuring module to enter the detection outer cover to form a closed measurement darkroom; the measuring module also comprises a photomultiplier, and the excitant needle group injects the excitant into the reaction cup through the excitant injection port to carry out chemiluminescence detection.

The measuring module is of a drawer type structure, only one motor (measuring module motor) is used for controlling the separation and the entrance of the inner box, and a magnet seat for attracting magnetic beads to stay at the bottom of the reaction cup is not arranged, so that the magnetic beads are kept at the lower part of the reaction cup during cleaning and transferring, the structure is simple, and the cost is low; when in work: the reaction cup is placed in the detection inner chamber by the clamping jaw, the detection inner chamber is controlled by the motor of the measurement module to enter the detection outer cover, a closed measurement dark chamber is formed, the reaction cup is right opposite to the photomultiplier, the excitant needle group injects the excitant into the reaction cup, and the injection process has a uniform mixing effect on internal magnetic beads, so that the follow-up chemiluminescence detection is facilitated.

Preferably, the quintuplet cup comprises a connecting sleeve and a plurality of cup grooves, the connecting sleeve is provided with a plurality of connecting holes corresponding to the cup grooves, and the cup grooves are respectively and vertically connected with the connecting holes in a one-to-one correspondence manner, so that the cup grooves are mutually independent.

The cup slots are connected together by the connecting sleeves and are mutually independent for placing the reaction cups and/or presetting reaction reagents, all reagents required by detection are integrated into the quintuplet cup, the use is convenient, the clinical detection time can be saved, meanwhile, the quintuplet cup is a disposable consumable in the detection process, the pollution caused by repeated cleaning and use of the reagents and the sample tubes or the test card boxes can be avoided, and the detection accuracy is improved.

Preferably, the quintuplet cup further comprises a cup sleeve, and the cup sleeve surrounds the outer walls of the plurality of cup grooves and is vertically connected with the connecting sleeve; the connecting sleeve further comprises a plurality of connecting parts, and the connecting parts are respectively arranged among the cup grooves and between the cup grooves and the cup sleeves.

The cup grooves are fixed with each other, so that the cup grooves cannot be inclined or misplaced in the detection process.

Drawings

The following further detailed description of embodiments of the invention is made with reference to the accompanying drawings:

FIG. 1 is a schematic view of a chemiluminescent immunoassay analyzer of the present invention;

FIG. 2 is a schematic diagram of a sample injection module in the chemiluminescence immunoassay analyzer of the present invention;

FIG. 3 is a schematic perspective view of the sample injection module of FIG. 2 with a second side baffle removed;

FIG. 4 is a top view of the sample injection module of FIG. 2;

FIG. 5 is a block diagram of the deadbolt assembly of FIG. 2;

FIG. 6 is a flow chart of a method of testing a sample using a quintuplet cup in accordance with the present invention;

FIG. 7 is a schematic perspective view of the quintuplet cup of FIG. 2;

FIG. 8 is a bottom view of the quintuplet cup of FIG. 7;

FIG. 9 is a view showing the structure of a reagent cuvette in a second cuvette among the quintuplet cuvettes of FIG. 7;

FIG. 10 is a block diagram of a sample cuvette in a five-cup well in the quintuplet cup of FIG. 7;

FIG. 11 is a schematic perspective view of an incubation magnetic separation cleaning module in a chemiluminescent immunoassay analyzer of the present invention;

FIG. 12 is a perspective view of the magnetic separation device of FIG. 11;

FIG. 13 is a schematic cross-sectional view of the magnetic separation apparatus of FIG. 12;

fig. 14 is a schematic view of the three-dimensional structure of the magnet holder of fig. 11;

FIG. 15 is a schematic cross-sectional perspective view of the magnet base of FIG. 11;

FIG. 16 is a schematic perspective view of the reaction plate assembly of FIG. 11;

FIG. 17 is a schematic sectional view of a two-stage transmission assembly of the apparatus for an incubation reaction disk of FIG. 16;

FIG. 18 is a perspective view of a two-stage actuator assembly of the apparatus of FIG. 16;

FIG. 19 is a schematic perspective view of a heating plate on the back surface of the incubation tray in the incubation reaction tray device of FIG. 16;

FIG. 20 is a perspective view of the cleaning device of FIG. 11;

FIG. 21 is a side perspective view of the cleaning needle attachment bracket of FIG. 20;

FIG. 22 is a perspective view of the cleaning needle assembly of FIG. 20;

FIG. 23 is a schematic perspective view of a horizontal motion assembly of a clamping jaw and a sampling needle module in the chemiluminescent immunoassay analyzer of the present invention;

FIG. 24 is a schematic perspective view of the vertical movement assembly of the clamping jaw and the sampling needle module in the chemiluminescent immunoassay analyzer of the present invention;

FIG. 25 is a perspective view of a clamping jaw and a sampling needle in the sampling needle module;

FIG. 26 is an internal configuration view of the sampling needle of FIG. 25;

FIG. 27 is a perspective view of a jaw and a jaw of a sampling needle module;

FIG. 28 is a perspective view of the cartridge of FIG. 27;

FIG. 29 is a perspective view of a sampling needle cleaning device in the jaw and sampling needle module;

FIG. 30 is a top view of the sampling needle cleaning device of FIG. 29;

FIG. 31 is a schematic perspective view of a measurement module of the chemiluminescent immunoassay analyzer of the present invention;

FIG. 32 is a structural diagram of a detection state of the measurement module of FIG. 31;

FIG. 33 is a side cross-sectional view of a finger device of the sample introduction module;

FIG. 34 is a partially enlarged top view of a sample introduction module;

wherein: 1-a base; 2-sample introduction module; 201-sample introduction device body; 2011-first support column; 2012 a second support column; 2013-a first side baffle; 2014-second side baffle; 2015-contact induction probe; 2016-connecting block; 202-ball plunger; 203-hand-poking rotating shaft; 204-a drive device; 2041-motor; 2042-lead screw; 205-a thumbwheel mechanism; 2051-second finger; 2052-first thumbpiece; 2053-connecting rod; 2054-spring fixing block; 2055-sample injection spring; 2056-optical coupling limiting piece; 2057-sliding block; 2058-guide rails; 2059-slide block fixing block; 206-fixing and pressing connecting blocks; 207-guide groove; 2071-vacant position of sample feeding groove; 208-optical couplers; 2081-an optocoupler support; 3-quintuplet cup; 301-connecting sleeve; 302-cup slot; 303-connecting hole; 304-a cup sleeve; 305-a connecting portion; 306-a card slot; 4-incubation magnetic separation cleaning module; 5-incubation reaction plate device; 501-a bottom plate; 502-incubation of the scaffold; 5021-a containing cavity; 503-incubation dish; 5031-outer lane reaction cup position; 5032-inner circle reaction cup position; 504-heating plates; 505-a temperature sensor; 506-two-stage transmission component part of the incubation disc; 507-a two-stage drive motor; 508-a primary transmission assembly; 509-a secondary drive assembly; 5081-primary driving wheel; 5082-primary driven wheel; 5091-secondary driving wheel; 5092-secondary driven wheel; 5010-incubation plate spindle; 5011-circuit board; 5012-temperature controller; 5013-relay; 6-a magnetic separation device; 601-arc push block; 602-a magnet holder; 6021-magnet holder; 6022-magnet fixing sleeve; 6023-internal magnet assembly; 6024-a spring; 6025-magnet; 6026-linear bearing support post; 603-a vertical plate; 604-a nut; 605-a horizontal plate; 606-a lead screw motor; 607-lead screw; 608-vertical small push block; 7-a cleaning device; 701-cleaning the bracket; 702-a cleaning needle connecting bracket; 703-washing the needle assembly; 704-a cleaning device motor; 705-fixed block; 706-a limit nut; 707-a connecting rod; 708-connecting the slider; 709-guide rail; 7010-an optocoupler; 7011-baffle plate; 7012-cleaning needles; 7013-leaf spring; 7014-liquid injection needle; 7015-liquid discharge needle; 7016-motor shaft screw; 8-clamping jaw and sampling needle module; 9-a kinematic assembly portion; 10-a clamping jaw; 1001-gripper arm connection block; 1002-chuck mounting a connecting piece; 1003-jaw mounting plate; 1004-chuck; 1005-torque measuring device; 1006-mirror chuck; 1007-a groove; 1008-chuck motor; 11-a sampling needle; 1101-a needle arm; 1102-needle arm upper cover; 1103-sampling arm cavity; 1104-an optical coupler; 1105-a liquid level sensor; 1106-sensing a baffle; 1107-spring retainer; 1108-a sampling needle spring; 1109-sensor chip; 11010-sliding sleeves; 11011-sleeve; 11012-insulating fixed ring; 11013-inner needle tube; 12-a sampling needle cleaning device; 1201-cleaning the stent with the sampling needle; 1202-washing pool; 1203-side liquid inlet; 1204-lower drain; 13-an up-and-down motion assembly; 1301-a sampling needle up-and-down movement motor; 1302-fixed block one; 1303-a guide shaft; 1304-guide shaft slide block I; 1305-a damping encoder; 1306-a sampling needle up-and-down movement assembly; 1307-jaw up-down motion assembly; 13071-motor for moving clamping jaw up and down; 13072-fixed block two; 13073-guide shaft slide block two; 14-a horizontal motion assembly; 1401-jaw horizontal motion motor; 1402-sampling needle spline shaft; 1403-jaw spline shaft; 1404-a sampling needle horizontal movement assembly; 14041-sampling needle horizontal movement motor; 1405-horizontal jaw movement assembly; 15-a measurement module; 1501-detecting the inner chamber; 1502-measuring the module motor; 1503-detecting the outer cover; 1504-sealing a measurement darkroom; 1505-reaction cup placing hole; 1506-a photomultiplier; 1507-excimer injection port; 1508-excimer needle set; 16-a switch; 17-a code scanner; 18-a power supply filter component; 19-a waste chute; 20-a scanner; 21-a multi-pump module; 22-valveless plunger pump assembly; 23-a plunger pump assembly; 24-printer.

Detailed Description

As shown in fig. 1, the chemiluminescence immunoassay analyzer of the present invention comprises a base 1, wherein the base 1 is provided with:

a sample introduction module 2, wherein the sample introduction module 2 comprises a quintuplet cup 3 (shown in fig. 2) loaded with a reagent;

an incubation magnetic separation cleaning module 4, as shown in FIG. 11, comprising an incubation reaction disk device 5, a magnetic separation device 6 and a cleaning device 7; the incubation reaction plate device 5 is used for incubating the reaction cups in the quintuplet cup 3 and keeping constant temperature reaction; the magnetic separation device 6 is used for adsorbing magnetic beads in the reaction cup; the cleaning device 7 is used for cleaning the magnetic beads in the reaction cup;

the clamping jaw and sampling needle module 8 comprises a motion component part 9, a clamping jaw 10, a sampling needle 11 and a sampling needle cleaning device 12; the motion component part 9 is composed of an up-and-down motion component 13 and a horizontal motion component 14, the horizontal motion component 14 is used for driving the sampling needle 11 and the clamping jaw 10 to move horizontally, and the up-and-down motion component 13 is used for enabling the sampling needle 11 and the clamping jaw 10 to move up and down; the clamping jaw 10 is used for transferring a sample reaction cup and a labeled antibody cup in the quintuplet cup 3, and the sampling needle 11 is used for transferring liquid in the quintuplet cup 3; the sampling needle cleaning device 12 is used for cleaning the sampling needle 11;

and a measuring module 15, wherein the measuring module 15 is used for carrying out chemiluminescence detection on the reaction cup.

In this embodiment, as shown in FIG. 1, the chemiluminescent immunoassay analyzer of the present invention also has a switch 16 for turning on or off the overall control of the analyzer; the code scanner 17 scans the five-linked cup by the code scanner 17, records information, and then places the five-linked cup into the instrument sample injection module 2; a power filter assembly 18, a waste tank 19 for loading waste, a scanner 20, an activator needle set 1508, a multi-pump module 21, a valveless plunger pump assembly 22, a plunger pump assembly 23, and a printer 24 for printing diagnostic results.

Fig. 2-4 show a sample injection module 2 of this embodiment, which includes a sample injection device body 201, a ball plunger 202 and a driving device 204, wherein the ball plunger 202 is connected to the sample injection device body 201, and the driving device 204 drives and controls the quintuplet cup 3 to move; the ball plunger 202 limits the movement of the quintuplet cup 3; the sample injection module 2 further comprises a hand dialing device 205, as shown in fig. 5, the hand dialing device 205 comprises a second hand dialing member 2051, a first hand dialing member 2052 and a connecting rod 2053, the second hand dialing member 2051 and the first hand dialing member 2052 are respectively arranged at two ends of the connecting rod 2053, a hand dialing rotating shaft 203 is arranged at one side of the first hand dialing member, the connecting rod 2053 is connected with a sample injection spring 2055 below the first hand dialing member 2052 through a spring fixing block 2054, one end of the sample injection spring 2055 is in press contact with the first hand dialing member 2052, and an optical coupling limiting sheet 2056 is connected below the other end of the sample injection spring 2055; the driving device 204 drives the quintuplet cup 3 to move through the hand-pulling device 205.

The hand pulling device 205 is arranged, so that the driving device 204 drives the quintuplet cup 3 to move by using the second hand pulling 2051 and the first hand pulling 2052, and meanwhile, the second hand pulling 2051 and the first hand pulling 2052 also have a limiting effect on the quintuplet cup 3, so that the movement of the quintuplet cup 3 and the position after the movement are better controlled; the arrangement of the optocoupler limiting piece 2056 can prompt the position of the hand dialing device 205; the driving device 204 comprises a slider fixing block 2059 and a guide rail 2058, and one side of the slider fixing block 2059 is sleeved and clamped with the guide rail 2058 to enable the slider fixing block 2059 to move on the guide rail 2058; the hand dialing device 205 is fixed on the slider fixing block 2059, and the slider fixing block 2059 drives the hand dialing device 205 to move along the guide rail 2058; the quintuplet cup 3 is placed between the second hand driver 2051 and the first hand driver 2052, and the quintuplet cup 3 is driven by the second hand driver 2051 and the first hand driver 2051 to move; the sliding block fixing block 2059 is matched with the guide rail 2058 to drive the hand poking device 205 to move along the guide rail 2058, so that the five-linked cup 3 moves back and forth along the guide rail 2058, and the movement of the five-linked cup 3 is favorably controlled; the sample feeding device body 201 comprises the first supporting column 2011, the second supporting column 2012, the first side baffle 2013 and the second side baffle 2014; the first side baffle 2013 and the second side baffle 2014 are arranged in parallel and connected between the first supporting column 2011 and the second supporting column 2012; the ball plunger 202 is mounted on the first side baffle 2013; the hand-pulling device 205 and the quintuplet cup 3 are arranged between the first side baffle 2013 and the second side baffle 2014 by arranging the first side baffle 2013 and the second side baffle 2014, and the lower end of the hand-pulling device 205 is exposed below the first side baffle 2013 and the second side baffle 2014 and is fixed on a slider fixing block 2059 at the outer side of the second side baffle 2014; a contact induction probe 2015 is connected beside the ball plunger 202 through a fixed compression connecting block 206, the fixed compression connecting block 206 is installed on the outer side of the first side baffle 2013, and the ball plunger 202 is enclosed in the space of the fixed compression connecting block 206; the driving device 204 is installed at the outer side of the second side baffle 2014, the driving device 204 further includes a motor 2041, a lead screw 2042 and a slider 2057, the motor 2041 is connected with the lead screw 2042, the slider 2057 is sleeved on the lead screw 2042, and the slider 2057 is connected with the slider fixing block 2059; the guide rails 2058 are mounted on the outer side of the second side barrier 2014; the code scanner 17 is arranged on the outer side of the first side baffle 2013 and on one side of the fixed compression connecting block 206; a code scanner 17 is arranged for recording the information of the sample to be tested in the quintuplet cup 3; the sample feeding module 2 further comprises an optical coupler 208, and the optical coupler 208 is arranged on the first support column 2011 through the optical coupler support 2081, is matched with the optical coupler limiting piece 2056 and is used for prompting the position of the hand dialing device 205; and a notch is formed in the second side baffle 2014 and is connected through a connecting block 2016. This advance kind module 2 still includes guide way 207 and advance kind groove vacancy position 2071, advance kind groove vacancy position 2071 is used for placing the pre-abandonment quintuplet cup 3, quintuplet cup 3 drives through the hand setting device slide in the guide way 207 extremely advance kind groove vacancy position 2071. After the mixing of the sample is completed, the hand-pulling device continues to drive the quintuplet cup 1 to move, the head of the first hand-pulling 2052 is raised to clamp the lower end angle of the handheld part of the quintuplet cup 3 to drive the quintuplet cup 3 to move under the action of the contraction of the sample feeding spring 2055 and the hand-pulling rotating shaft 203, the quintuplet cup 3 slides in the guide groove 207, and when the vacant position 2071 of the sample feeding groove is reached, the sample is discharged out of the instrument through the waste material groove downwards.

As shown in fig. 7 and 8, the quintuplet cup 3 includes a connecting sleeve 301 and a plurality of cup slots 302, the connecting sleeve 301 is provided with a plurality of connecting holes corresponding to the cup slots 302, and the cup slots 302 are respectively vertically connected with the connecting holes 303 in a one-to-one correspondence manner, so that the cup slots 302 are independent from each other; the quintuplet cup 3 further comprises a cup sleeve 304, and the cup sleeve 304 surrounds the outer walls of the plurality of cup grooves 302 and is vertically connected with the connecting sleeve 301; the connecting sleeve 301 further comprises a plurality of connecting parts 305, and the connecting parts 305 are respectively arranged between the plurality of cup slots 302 and between the cup slots 302 and the cup sleeve 304.

In this embodiment, specifically, the cup groove 302 of the quintuplet cup 3 is divided into a first cup groove, a second cup groove, a third cup groove, a fourth cup groove and a fifth cup groove, and the clamping groove 306 is located between the first cup groove and the second cup groove; the diameters of the second cup groove and the fifth cup groove are larger than those of the first cup groove, the third cup groove and the fourth cup groove, and the second cup groove and the fifth cup groove are used for placing the reaction cups; the five-link cup 3 is provided with a clamping groove 306 between the first cup groove and the second cup groove, is clamped with the first ball plunger 202 and is used for limiting the five-link cup 3; a reagent reaction cup (as shown in figure 9) is arranged in the second cup groove in the quintuplet cup 3, and the reagent reaction cup is designed to be light-proof and is internally provided with a labeled antibody; the reaction cup is placed in the fifth cup groove and is a sample reaction cup to be tested (as shown in figure 10), and the other cup grooves directly load the reagent therein.

Wherein, the sample injection process by using the sample injection module 2 is as follows: firstly, placing a reagent reaction cup in a second cup groove in a quintuplet cup 3, and filling a labeled antibody in the reagent reaction cup; a reaction cup is placed in the fifth cup groove and is a sample reaction cup to be tested, and reagents are directly loaded in other cup grooves; the quintuplet cup 3 is scanned by the code scanner 17 to input information of a sample to be tested, then the quintuplet cup 3 is placed into a sample inlet of the sample injection module 2, the motor 2041 is started, the motor 2041 drives the slide block 2057 to move on the guide rail 2058 through the lead screw 2042, and synchronously, the slide block 2057 drives the hand dialing device 205 connected with the slide block to move along the guide rail 2058, so that the quintuplet cup 3 is driven to move together; when the front end of the quintuplet cup 3 reaches the ball plunger 202, the clamping groove 306 at the front end of the quintuplet cup 3 is clamped with the ball plunger 202, and the arrival of a manual putting position is prompted; the contact induction probe 2015 senses that the quintuplet cup 1 is in place, and the display screen of the detection instrument indicates that the quintuplet cup 3 is in place at the moment, so that the next operation can be performed; after the reaction reagent, the sample, the magnetic beads and the like in the quintuplet cup 3 are injected with the sample, the detection instrument controls the quintuplet cup 3 to continuously move forwards, and the reaction reagent, the sample, the magnetic beads and the like reach the vacant position of the injection groove and are discharged out of the detection instrument through the waste hopper; after the quintuplet cup 3 is discarded, the motor 2041 moves to drive the hand-shifting device 205 to move forward, the optocoupler 208 on the first support column 2011 is matched with the optocoupler limiting piece 2056 fixedly connected with the second hand-shifting piece 2052 to prompt the hand-shifting device 205 to reach the initial position, and the quintuplet cup 3 can be fed again.

As shown in fig. 6, the method for testing a sample by using a quintuplet cup specifically comprises the following steps:

(1) a sample inlet of a detection instrument (sample introduction module) pops up a quintuplet cup receiving device;

(2) the quintuplet cup 3 is arranged in the receiving device, a sample to be detected is added into the sample reaction cup and placed into a fifth cup groove, a labeled antibody and reagent reaction cup is added into a second cup groove, a labeled antibody buffer solution is preset in the first cup groove, a reaction buffer solution is preset in the third cup groove, and magnetic beads are preset in the fourth cup groove;

(3) the quintuplet cup 3 enters from a sample inlet of the detection instrument;

(4) firstly, absorbing a labeled antibody buffer solution in a first cup groove by using a combined cleaning needle, adding the labeled antibody buffer solution into a labeled antibody in the reagent reaction cup, and uniformly mixing;

(5) after the combined cleaning needle is cleaned, absorbing the reaction buffer solution in the third cup groove, adding the reaction buffer solution into the fourth cup groove, uniformly mixing the reaction buffer solution with the magnetic beads in the fourth cup groove, and absorbing the uniformly mixed liquid;

(6) adding the uniformly mixed liquid in the step (5) into a sample reaction cup, and uniformly mixing;

(7) then grabbing the reagent reaction cup and the sample reaction cup to be placed in a turntable, and incubating the sample reaction cup;

(8) after the combined cleaning needle is cleaned, sucking liquid in a reagent reaction cup on the turntable, flushing the liquid into a sample reaction cup, uniformly mixing, and then incubating;

(9) the combined washing needle was washed and the sample cuvette was transferred to a dark room, developed and the results read.

As shown in fig. 16-19, the device 5 for incubation reaction disc comprises a bottom plate 501, an incubation frame 502 is disposed on the bottom plate 501, an incubation disc 503 is disposed on the incubation frame 502, and a heating plate 504 and a temperature sensor 505 are disposed on the back (or reverse) of the incubation disc 503 (as shown in fig. 19); an incubation disk two-stage transmission component part 506 is arranged below the incubation disk 503, and the incubation disk two-stage transmission component part 506 is composed of a two-stage transmission motor 507, a first-stage transmission component 508 and a second-stage transmission component 509; wherein, the two-stage transmission motor 507 drives a primary driving wheel 5081 in the primary transmission assembly 508, the primary driving wheel 5081 drives a primary driven wheel 5082, the primary driven wheel 5082 and a secondary driving wheel 5091 coaxially rotate, the secondary driving wheel 5091 drives a secondary driven wheel 5092, the secondary driven wheel 5092 and an incubation disc rotating shaft 5010 coaxially rotate, and the incubation disc rotating shaft 5010 drives the incubation disc 503 to rotate; the primary driven wheel 5082 and the secondary driven wheel 5092 have larger radii than the primary driving wheel 5081 and the secondary driving wheel 5091; the incubation tray 503 is provided with an outer ring reaction cup position 5031 and an inner ring reaction cup position 5032 which are both provided with a plurality of cup holes for placing reaction cups; the incubation holder 502 has a housing cavity 5021; the two-stage transmission motor 507 is located in the accommodating cavity 5021, and a circuit board 5011, a temperature controller 5012 and a relay 5013 are further arranged in the accommodating cavity 5021.

The magnetic separation device 6 comprises an arc-shaped push block 601 and a magnet frame 602, as shown in fig. 12-15; the magnet frame 602 is composed of a plurality of magnet seats 6021, the magnet seats 6021 are composed of magnet fixing sleeves 6022 and internal magnet assemblies 6023, and the magnet fixing sleeves 6022 are fixed on the magnet frame 602 through bolts; the inner magnet assembly 6023 comprises a spring 6024 and a terminal magnet 6025; the arc-shaped push block 601 is fixedly connected with a linear bearing support 6026; a vertical plate 603 is arranged below the arc-shaped push block 601, the vertical plate 603 is connected with a transverse plate 605 fixedly provided with a screw 604, the vertical plate 603 drives a screw 607 by a screw motor 606, and the screw 604 is sleeved on the screw 607 to enable the vertical plate 603 to move up and down; the outer edge of the arc-shaped push block 601 is provided with a small vertical push block 608, and the small vertical push block 608 is provided with a slope structure, so that the magnet 6025 at the tail end is contacted with the reaction cup by generating pushing force on the spring 6024 in the magnet seat 6021 through the slope structure.

As shown in fig. 20 to 22, the cleaning device 7 of this embodiment includes a cleaning holder 701, a cleaning needle connecting holder 702 is disposed on the cleaning holder 701, and a cleaning needle assembly 703 is mounted on the cleaning needle connecting holder 702; the cleaning device further comprises a cleaning device motor 704, and the cleaning device motor 704 is fixedly connected with a fixing block 705; a limit nut 706 is further arranged on the cleaning support 701, the fixed block 705 is connected with a connecting rod 707, the connecting rod 707 is fixedly connected with a connecting sliding block 708, and the connecting sliding block 708 is connected with the cleaning needle connecting support 702; the connecting slide block 708 moves up and down along the guide rail 709 on the cleaning bracket 701; the side part of the cleaning needle connecting bracket 701 is provided with an optical coupler 7010 and a baffle 7011; the cleaning needle assembly 703 is provided with a plurality of cleaning needles 7012, and the cleaning needles 7012 are connected with spring plates 7013; the cleaning needle 7012 is composed of a liquid inlet needle 7014 and a liquid discharge needle 7015, the liquid inlet needle 7014 is shorter than the liquid discharge needle 7015, a needle head is bent to the liquid discharge needle 7015, and a motor rotating shaft nut 7016 is further connected to the fixing block 705.

As shown in fig. 23-24, the horizontal motion assembly 14 in this embodiment includes a sampling needle horizontal motion assembly 1404 and a jaw horizontal motion assembly 1405, wherein the sampling needle horizontal motion assembly 1404 is composed of a sampling needle horizontal motion motor 14041 and a sampling needle spline shaft 1402, and the jaw horizontal motion assembly 1405 is composed of a jaw horizontal motion motor 1401 and a jaw spline shaft 1403; the sampling needle spline shaft 1402 passes through the jaw spline shaft 1403, so that the sampling needle 11 and the jaw 10 are assembled in combination; the sampling needle horizontal movement motor 14041 drives the sampling needle spline shaft 1402, and the sampling needle spline shaft 1402 is connected with the needle arm 1101 so as to drive the sampling needle 11 to move horizontally; the clamping jaw horizontal movement motor 1401 drives the clamping jaw 10 to move horizontally by driving the clamping jaw spline shaft 1403; the up-down movement assembly 13 comprises a sampling needle up-down movement assembly 1301 and a clamping jaw up-down movement assembly 1307, wherein the sampling needle up-down movement assembly 1301 comprises a sampling needle up-down movement motor 1301, a first fixed block 1302 and a first guide shaft slider 1304, the sampling needle up-down movement motor 1301 enables the first guide shaft slider 1304 to move up and down along a guide shaft 1303 through the first fixed block 1302, and the first guide shaft slider 1304 is fixedly connected with a sampling needle spline shaft 1402, so that the sampling needle 11 can move up and down; a damping encoder 1305 is arranged on the sampling needle up-and-down movement motor 1301; the clamping jaw up-and-down movement assembly 1307 comprises a clamping jaw up-and-down movement motor 13071, a second fixed block 13072 and a second guide shaft slider 13073, wherein the clamping jaw up-and-down movement motor 13071 enables the second guide shaft slider 13073 to move up and down along the guide shaft 1303 through the second fixed block 13072, and the second guide shaft slider 13073 is fixedly connected with the clamping jaw spline shaft 1403, so that the clamping jaw 10 can move up and down.

As shown in fig. 27 and 28, the clamping jaw 10 comprises the clamping jaw spline shaft 1403, a clamping jaw arm connecting block 1001, a clamping jaw mounting connecting piece 1002, a clamping jaw mounting plate 1003, a clamping jaw 1004, a torque measuring device 1005 and a mirror-image clamping jaw 1006; the clamping head 1004 is provided with a groove 1007, and the mirror-mounted clamping head 1006 is matched with the clamping head 1004 for grabbing a reaction cup; the mirror-mount chuck 1006 and the chuck 1004 are both connected to the chuck mount connector 1002; the chuck mounting connecting piece 1002 is connected with the clamping jaw mounting plate 1003, and a chuck motor 1008 and the torque measuring device 1005 are arranged on the clamping jaw mounting plate 1003; the jaw arm connecting block 1001 is connected to the jaw mounting plate 1003, and the jaw spline shaft 1403 is located on the jaw arm connecting block 1001.

As shown in fig. 25 and 26, the sampling needle 11 has a needle arm 1101, the needle arm 1101 has a needle arm upper cover 1102 thereon, and the needle arm 1102 and the needle arm 1101 are fitted to form a sampling arm cavity 1103; an optical coupler 1104, a liquid level sensor 1105, an induction baffle 1106 and a spring baffle 1107 are arranged in the sampling arm cavity 1103; a sampling needle spring 1108, an induction sheet 1109 and a sliding sleeve 11010 are sequentially arranged below the spring blocking piece 1107; a sleeve 11011, an insulating fixed ring 11012 and an inner needle tube 11013 are sequentially arranged below the sliding sleeve 11010.

The sampling needle cleaning device 12 comprises a sampling needle cleaning bracket 1201 and a cleaning pool 1202; the cleaning tank 1202 is provided with a side inlet 1203 and a lower outlet 1204, as shown in FIGS. 29 and 30.

The measuring module 15 in this embodiment is shown in fig. 31 and 32, and includes an inspection inner chamber 1501, the clamping jaws 10 place the reaction cup into the inspection inner chamber 1501, the inspection inner chamber 1501 has a reaction cup placing hole 1505, and the measuring module motor 1502 controls the inspection inner chamber 1501 to enter an inspection housing 1503 to form a closed measuring darkroom 1504; the measurement module 15 further includes a photomultiplier 1506, and an activator needle set 1508 injects an activator into the reaction cup through an activator injection port 1507 for chemiluminescence detection, and the detection result is printed by the printer 24.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications, such as changes in the position of various elements mounted on a base, may be made without departing from the spirit and scope of the invention, and these changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a chemiluminescence immunoassay appearance, including the base, its characterized in that be provided with on the base:

2. The chemiluminescent immunoassay analyzer of claim 1, wherein the sample module further comprises a sample device body, a ball plunger and a driving device, wherein the ball plunger is connected to the sample device body, and the driving device drives and controls the quintuplet cup to move; the ball plunger is used for limiting the quintuplet cup.

3. The sample introduction device according to claim 2, wherein the sample introduction module further comprises a hand dialing device, the hand dialing device comprises a first hand dialing part, a second hand dialing part and a connecting rod, the first hand dialing part and the second hand dialing part are respectively arranged at two ends of the connecting rod, and a hand dialing rotating shaft is arranged at one side of the first hand dialing part; the connecting rod is connected with a sample injection spring below the first shifting handle through a spring fixing block, one end of the sample injection spring is in press contact with the first shifting handle, and an optocoupler limiting sheet is connected below the other end of the sample injection spring; the driving device drives the quintuplet cup to move through the hand poking device.

4. The chemiluminescent immunoassay analyzer of claim 1 wherein the incubation reaction tray device comprises a bottom plate, wherein an incubation bracket is arranged on the bottom plate, the incubation tray is positioned on the incubation bracket, and a heating plate and a temperature sensor are arranged on the back surface of the incubation tray; an incubation disc two-stage transmission component part is arranged below the incubation disc and consists of a two-stage transmission motor, a first-stage transmission component and a second-stage transmission component; the two-stage transmission motor drives a first-stage driving wheel in the first-stage transmission assembly, the first-stage driving wheel drives a first-stage driven wheel, the first-stage driven wheel and a second-stage driving wheel rotate coaxially, the second-stage driving wheel drives a second-stage driven wheel, and the second-stage driven wheel and the rotary shaft of the incubation plate rotate coaxially. The incubation disc rotating shaft drives the incubation disc to rotate; the radiuses of the first-stage driven wheel and the second-stage driven wheel are larger than those of the first-stage driving wheel and the second-stage driving wheel.

5. The chemiluminescent immunoassay analyzer of claim 4 wherein the incubation tray has an outer reaction cup position and an inner reaction cup position, each of which is provided with a plurality of cup holes for placing reaction cups.

6. The chemiluminescent immunoassay analyzer of claim 5 wherein the incubation rack has a receiving cavity; the two-stage transmission motor is positioned in the containing cavity, and a circuit board, a relay and a temperature controller are further arranged in the containing cavity.

7. The chemiluminescent immunoassay analyzer of claim 1, wherein the magnetic separation device comprises an arc-shaped push block and a magnet frame; the magnet frame consists of a plurality of magnet seats, each magnet seat consists of a magnet fixing sleeve and an internal magnet assembly, and the magnet fixing sleeves are fixed on the magnet frame through bolts; the internal magnet assembly comprises a spring and a magnet at the end; the arc-shaped pushing block is fixedly connected with a linear bearing support.

8. The chemiluminescence immunoassay analyzer of claim 7, wherein a vertical plate is arranged below the arc-shaped push block, the vertical plate is connected with a transverse plate fixedly provided with a screw nut, the vertical plate drives the screw nut by a screw rod motor, and the screw nut is sleeved on the screw rod to enable the vertical plate to move up and down; the outer edge of the arc-shaped push block is provided with a small vertical push block, the small vertical push block is provided with an inclined plane structure, and the inclined plane structure generates thrust on the spring in the magnet seat to enable the magnet at the tail end to contact the reaction cup.

9. The chemiluminescent immunoassay analyzer of claim 1, wherein the cleaning device comprises a cleaning bracket, a cleaning needle connecting bracket is arranged on the cleaning bracket, and a cleaning needle assembly is arranged on the cleaning needle connecting bracket; the cleaning device further comprises a cleaning device motor, and the cleaning device motor is fixedly connected with a fixing block; the cleaning needle is characterized in that the cleaning support is also provided with a limiting nut, the fixed block is connected with a connecting rod, the connecting rod is fixedly connected with a connecting slide block, and the connecting slide block is connected with the cleaning needle connecting support; the connecting slide block moves up and down along the guide rail on the cleaning bracket.

10. The chemiluminescent immunoassay analyzer of claim 9, wherein the side of the cleaning needle connecting bracket is provided with an optical coupling baffle plate; the cleaning needle assembly is provided with a plurality of cleaning needles which are connected with spring pieces; the cleaning needle consists of a liquid inlet needle and a liquid discharge needle, wherein the liquid inlet needle is shorter than the liquid discharge needle, and the needle head is bent towards the liquid discharge needle.

11. The chemiluminescent immunoassay analyzer of claim 1, wherein the horizontal motion assembly comprises a sampling needle horizontal motion assembly and a jaw horizontal motion assembly, wherein the sampling needle horizontal motion assembly is composed of a sampling needle horizontal motion motor and a sampling needle spline shaft, and the jaw horizontal motion assembly is composed of a jaw horizontal motion motor and a jaw spline shaft; the sampling needle spline shaft penetrates through the clamping jaw spline shaft, so that the sampling needle and the clamping jaw are assembled in a combined manner; the sampling needle horizontal movement motor drives a sampling needle spline shaft, and the sampling needle spline shaft is connected with a needle arm and then drives the sampling needle to move horizontally; the clamping jaw horizontal movement motor drives the clamping jaw to move horizontally by driving the clamping jaw spline shaft; the up-and-down movement assembly comprises a sampling needle up-and-down movement assembly and a clamping jaw up-and-down movement assembly, wherein the sampling needle up-and-down movement assembly comprises a sampling needle up-and-down movement motor, a first fixing block and a first guide shaft sliding block; a damping encoder is arranged on the sampling needle up-and-down movement motor; the clamping jaw up-and-down movement assembly comprises a clamping jaw up-and-down movement motor, a second fixing block and a second guide shaft sliding block, the clamping jaw up-and-down movement motor enables the second guide shaft sliding block to move up and down along the guide shaft through the second fixing block, and the second guide shaft sliding block is fixedly connected with the clamping jaw spline shaft, so that the clamping jaw can move up and down.

12. The chemiluminescent immunoassay analyzer of claim 11, wherein the jaw comprises the jaw spline shaft, and further comprises a jaw arm connecting block, a chuck mounting connector, a jaw mounting plate, a chuck, a torque meter and a mirror chuck; the chuck is provided with a groove, and the mirror chuck is matched with the chuck to grab the reaction cup; the mirror chuck and the chuck are both connected to the chuck mounting connecting piece; the chuck mounting connecting piece is connected with the clamping jaw mounting plate, and a chuck motor and the torque measuring device are arranged on the clamping jaw mounting plate; the clamping jaw arm connecting block with the clamping jaw mounting panel is connected, just the clamping jaw integral key shaft is located on the clamping jaw arm connecting block.

13. The chemiluminescent immunoassay analyzer of claim 12 wherein the sampling needle has a needle arm with a needle arm upper cover thereon, the needle arm upper cover and the needle arm being fitted to form a sampling arm cavity; an optical coupler, a liquid level sensor, an induction baffle and a spring baffle are arranged in the sampling arm cavity; a sampling needle spring, an induction sheet and a sliding sleeve are sequentially arranged below the spring retaining sheet; and a sleeve, an insulating fixing ring and an inner needle tube are sequentially arranged below the sliding sleeve.

14. The chemiluminescent immunoassay analyzer of claim 1, wherein the sampling needle cleaning device comprises a sampling needle cleaning bracket and a cleaning pool; the cleaning pool is provided with a side liquid inlet and a lower liquid outlet.

15. The chemiluminescent immunoassay analyzer of claim 1, wherein the measuring module comprises an inner detection chamber, the clamping jaws place the reaction cup into the inner detection chamber, and the measuring module motor controls the inner detection chamber to enter the detection housing to form a closed measurement darkroom; the measuring module also comprises a photomultiplier, and the excitant needle group injects the excitant into the reaction cup through the excitant injection port to carry out chemiluminescence detection.

16. The chemiluminescence immunoassay analyzer of claim 3, wherein the quintuplet cup comprises a connecting sleeve and a plurality of cup grooves, the connecting sleeve is provided with a plurality of connecting holes corresponding to the cup grooves, and the cup grooves are respectively vertically connected with the connecting holes in a one-to-one correspondence manner, so that the cup grooves are mutually independent.

17. The chemiluminescent immunoassay analyzer of claim 16, wherein the quintuplet cup further comprises a cup sleeve, wherein the cup sleeve is vertically connected with the connecting sleeve around the outer wall of the plurality of cup grooves; the connecting sleeve further comprises a plurality of connecting parts, and the connecting parts are respectively arranged among the cup grooves and between the cup grooves and the cup sleeves.