CN216747755U

CN216747755U - Full-automatic fluorescent quantitative analyzer

Info

Publication number: CN216747755U
Application number: CN202122516152.5U
Authority: CN
Inventors: 孙月鹏; 彭湃; 周伟伟
Original assignee: Zhongxiu Technology Co ltd
Current assignee: Zhongxiu Technology Co ltd
Priority date: 2021-10-19
Filing date: 2021-10-19
Publication date: 2022-06-14
Anticipated expiration: 2031-10-19

Abstract

The utility model discloses a full-automatic fluorescence quantitative analyzer, which comprises a complete machine bottom plate and a control system; a sample feeding device, a sample adding device, a buffer reaction device, a kit fixing device, a reagent card loading and moving device, an incubation reaction disc device and a detection device are arranged on the bottom plate of the whole machine; the kit fixing device is used for providing a reagent card required by the detection item; the reagent card loading and moving device is used for obtaining a reagent card and transferring the reagent card to the incubation reaction disc device; the sample feeding device is used for providing a sample for the sample feeding device; the sample adding device transfers the sample to the buffer reaction device to be mixed uniformly, and the mixed sample is added into the corresponding reagent card to finish sample adding; and the incubation reaction plate device is used for reacting the reagent card for sample adding and sending the reacted reagent card to the detection device. The utility model has the beneficial effects that: the problems that in the prior art, an in-vitro quantitative detection instrument is long in project detection time, long in space stroke, and inconvenient to operate due to the fact that equipment needs to be opened for replacing a kit and the operation mode is complicated are solved.

Description

Full-automatic fluorescent quantitative analyzer

Technical Field

The utility model relates to the technical field of fluorescence detection equipment, in particular to a full-automatic fluorescence quantitative analyzer.

Background

In order to meet the requirement of the current medical industry on Point-of-care testing (Point-of-care testing), achieve the aim of quickly obtaining an accurate test result, remove complicated operation procedures, reduce the influence of errors or errors caused by manual operation on the result, and improve the test speed, a full-automatic fluorescence immunoassay quantitative analyzer is an inevitable trend of the current instrument development.

In the prior art, the in-vitro quantitative detection instrument has the advantages of long item detection time, complex internal structure, long space stroke, increased failure rate of equipment, equipment opening for replacing the kit, complex loading mode of the kit and increased operation inconvenience.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problems that in the prior art, an in-vitro quantitative detection instrument has long item detection time, a complex internal structure and a long space stroke, increases the failure rate of equipment, needs to open the equipment for replacing a kit, and is inconvenient to operate due to a complex loading mode of the kit, and provides a full-automatic fluorescence quantitative analyzer.

The purpose of the utility model is realized by the following technical scheme: a full-automatic fluorescence quantitative analyzer comprises a complete machine bottom plate and a control system; the whole machine bottom plate is provided with a sample introduction device, a sample adding device, a buffer reaction device, a kit fixing device, a reagent card loading and moving device, an incubation reaction disc device and a detection device; the kit fixing device is used for providing a reagent card required by a detection item; the reagent card loading and moving device is used for acquiring a reagent card and moving the reagent card to the incubation reaction disc device; the sample feeding device is used for providing a sample for the sample feeding device; the sample adding device transfers the sample to the buffer reaction device to be mixed uniformly, and the mixed sample is added into the corresponding reagent card to finish sample adding; the incubation reaction disc device is used for reacting the reagent card added with the sample and sending the reacted reagent card to the detection device;

furthermore, the sample feeding device comprises a sample feeding platform; the sample introduction platform comprises a to-be-detected area, a sampling area, a detected area and an emergency treatment area; the sample feeding platform is also provided with a first sample feeding motion mechanism, a second sample feeding motion mechanism, a third sample feeding motion mechanism and a fourth sample feeding motion mechanism; the first sample feeding motion mechanism pushes the test tube rack to move to a to-be-detected area; the second sample introduction movement mechanism pushes the test tube rack to move from the to-be-detected area to the sampling area, and pushes the test tube rack after sampling is finished to the detected area continuously; the third sample feeding movement mechanism pushes the test tube rack out of the detected area; the fourth sample feeding motion mechanism is used for finishing detection when an emergency detection sample enters and exits the emergency treatment area;

furthermore, a shaking device is also arranged on the bottom plate of the whole machine; the shaking device is arranged in front of the sample feeding device and used for shaking the test tube sample on the sampling position before sample feeding; the shaking device comprises a rotating component, an up-and-down moving mechanism, a front-and-back moving mechanism and a mounting plate; the mounting plate is fixed on the whole machine bottom plate; the up-down moving mechanism is fixed on the mounting plate; the front-back moving mechanism is fixed on a sliding block of the up-down moving mechanism; the rotating assembly is fixed on a sliding block of the front-back moving mechanism; the rotating assembly is also provided with a test tube rack clamping hand;

furthermore, the sample adding device comprises a sample sucking needle part and a needle arm supporting component; the sample sucking needle part is provided with a sample sucking needle; the sample sucking needle part is used for driving a sample sucking needle to suck samples on the test tube rack; the needle arm supporting assembly is connected with the sample sucking needle; the needle arm supporting assembly is used for driving the sample sucking needle to reciprocate between the incubation reaction disc and the washing device;

furthermore, the washing device comprises a washing station, a first diaphragm pump, a second diaphragm pump, an injector, a first electromagnetic valve, a second electromagnetic valve, a cleaning liquid tank, a pure water tank and a waste water tank; the first diaphragm pump is respectively connected to the cleaning liquid tank and the pure water tank through a first electromagnetic valve; the second electromagnetic valve is arranged between the injector and the first diaphragm pump; the second solenoid valve is also connected to a washing station; one end of the second diaphragm pump is connected with the washing station, and the other end of the second diaphragm pump is connected with the waste water tank;

further, the buffer reaction device comprises a buffer solution part and a blending box part; the buffer part comprises a buffer liquid bottle and a first driving mechanism; the first driving mechanism is used for transferring the corresponding buffer liquid bottle to the lower part of the sample sucking needle; the blending box part comprises a blending box and a second driving mechanism; the second driving mechanism is used for transferring the blending box to be used to the lower part of the sample adding needle;

further, the kit fixing device comprises a kit clamping chamber part, a micro-motion sensor and an adsorption part; the bottom of the reagent box clamping bin is embedded in the bottom plate of the whole machine; the reagent box card bin part is provided with a plurality of card bins, and the bottom of each card bin is provided with a card bin outlet; the micro-motion sensor is fixed on one side surface of the upper reagent box card chamber part; each card bin corresponds to a micro-motion sensor; the magnet is arranged on one side surface of the cartridge part of the reagent box; each card bin corresponds to two adsorption pieces;

furthermore, the reagent card loading and moving device comprises an X-axis moving device and a Y-axis moving device; the X-axis moving device is fixed on a sliding block of the Y-axis moving device; a reagent card moving channel is arranged on the X-axis moving device; the card inlet of the reagent card moving channel is aligned with the card outlet of the card bin of the reagent box fixing device under the driving of the Y-axis moving device; a hook-and-clamp moving part is also arranged on the X axis;

further, the incubation reaction disc device comprises a heat preservation shell, a rotary disc and a rotary disc driving mechanism; the turntable driving mechanism drives the turntable to rotate in the heat-insulating shell; the turntable driving mechanism is fixed on the detection device and is vertically matched with the detection device; the heat-insulating shell is provided with a sample adding hole;

the detection device comprises a light path box, a light path box supporting component and a detection driving mechanism; the light path box is fixed on the light path box supporting component; the light path box supporting component is provided with a light path reagent card moving channel; the light path reagent card moving channel is in butt joint with a card outlet of the incubation reaction disc device.

The utility model has the following advantages:

the utility model relates to a full-automatic fluorescence quantitative analyzer, which is characterized in that a sample feeding device, a shaking device, a sample adding device, a buffer reaction device, a washing device, a kit fixing device, a reagent card loading and moving device, an incubation reaction disc device, a detection device and a system control module are organically combined together under the control of the system control module, and all devices are mutually matched to jointly achieve the purpose of automatically carrying out immune quantitative analysis. The sample to be detected is placed on the sample introduction platform by an operator, and the detection result is directly finished without any operation. Further, when the reagent cartridge is replaced, the used reagent cartridge is directly taken out and a new reagent cartridge is inserted without any complicated operation such as opening the apparatus. The internal structure of the fluorescence quantitative analyzer is deeply designed from installation, debugging and test stroke, so that the detection time of a project is shortened, and the stability of equipment is improved.

Drawings

FIG. 1 is a schematic diagram of an isometric structure of a fully automatic fluorescence quantitative analyzer.

FIG. 2 is a schematic diagram of the front view structure of the fully automatic fluorescence quantitative analyzer.

FIG. 3 is a schematic left view of the automatic fluorescence quantitative analyzer.

FIG. 4 is a right side view schematic diagram of the fully automatic fluorescence quantitative analyzer.

FIG. 5 is a top view of a sample injection device of the fully automatic fluorescence quantitative analyzer.

Fig. 6 is a schematic diagram of the emergency position when the sample introduction device of the full-automatic fluorescence quantitative analyzer is used for installing an emergency sample.

FIG. 7 is a schematic diagram of a sample injection device of the full-automatic fluorescence quantitative analyzer.

FIG. 8 is a schematic diagram of the shaking device of the full-automatic fluorescence quantitative analyzer.

FIG. 9 is a schematic view of the sample application device of the full-automatic fluorescence quantitative analyzer.

FIG. 10 is a schematic view of the structure of a buffer reaction device of the fully automatic fluorescence quantitative analyzer.

FIG. 11 is an exploded view of a buffer reaction device of the full-automatic fluorescence quantitative analyzer.

FIG. 12 is a schematic view of the washing apparatus of the fully automatic fluorescence quantitative analyzer.

FIG. 13 is a schematic view of the structure of a cartridge fixing device of the full-automatic fluorescence quantitative analyzer.

FIG. 14 is a schematic diagram of the structure of the automatic reagent card loading and moving device of the full-automatic fluorescence quantitative analyzer.

Fig. 15 is a schematic structural view of an X-axis moving device of the reagent card automatic loading moving device.

FIG. 16 is a schematic diagram of the top view of the automatic fluorescence quantitative analyzer with the internal device.

FIG. 17 is a front view of the automatic fluorescence quantitative analyzer with the internal device.

FIG. 18 is a schematic view of the structure of an incubation reaction plate device and a detection device.

FIG. 19 is a schematic top view of the incubation reaction plate device and the detection device.

FIG. 20 is a front view of the reaction plate device and the detection device.

In the figure: 1. a whole machine bottom plate; 2. a sample introduction device; 2-1, a sample introduction platform; 2-1-1, a region to be detected; 2-1-2. a sampling area; 2-1-3, examined area; 2-1-4, emergency treatment area; 2-2, a first sample feeding motion mechanism; 2-3, a second sample feeding motion mechanism; 2-4, a third sample feeding movement mechanism; 2-5, a fourth sample feeding motion mechanism; 3. shaking up the device; 3-1. rotating the assembly; 3-1-1, clamping the test tube rack; 3-2, an up-down moving mechanism; 3-3, a back-and-forth movement mechanism; 3-4, mounting a plate; 4. a sample adding device; 4-1, a sample sucking needle part; 4-1-1, a sample sucking needle; 4-2, a needle arm support assembly; 5. a buffer reaction device; 5-1, buffer part; 5-1-1. buffer solution bottle; 5-1-2. a first driving mechanism; 5-2, mixing box part 5-2; 5-2-1, mixing box; 5-2-2. a second driving mechanism; 6. a washing device; 6-1. a washing station; 6-2. a first diaphragm pump; 6-3. a second diaphragm pump; 6-4, injector 6; 6-5, a first electromagnetic valve; 6-6. a second electromagnetic valve; 6-7, a cleaning liquid tank; 6-8, a pure water tank; 6-9. a waste water tank; 7. a kit fixing device; 7-1, a reagent box card chamber part; 7-1-1, discharging the card from the card bin; 7-2. a micro-motion sensor; 7-3, an adsorption piece; 8. a reagent card loading mobile device; 8-1. an X-axis moving device; 8-1-1, hooking and clamping the moving part; 8-1-2, reagent card moving channel; 8-2. Y-axis moving device; 9. incubating the reaction plate device; 9-1, a heat preservation shell; 9-1-1. a sample adding hole; 9-2. a turntable; 9-3. a turntable driving mechanism; 10. a detection device; 10-1. light path box; 10-2. an optical path box supporting component; 10-3, detecting a driving mechanism; 11. and (5) controlling the system.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when the products of the present invention are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are used only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element that is referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used solely to distinguish one from another, and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1: as shown in fig. 1 to 20, a full-automatic fluorescence quantitative analyzer comprises a complete machine base plate 1 and a control system 11; the whole machine bottom plate 1 is provided with a sample feeding device 2, a sample feeding device 4, a buffer reaction device 5, a kit fixing device 7, a reagent card loading and moving device 8, an incubation reaction plate device 9 and a detection device 10; the kit fixing device 7 is used for providing a reagent card required by a detection item; the reagent card loading and moving device 8 is used for obtaining reagent cards and transferring the reagent cards to the incubation reaction plate device 9; the sample feeding device 2 is used for providing a sample to the sample feeding device 4; the sample adding device 4 transfers the sample to the buffer reaction device 5 to be uniformly mixed, and the uniformly mixed sample is added into the corresponding reagent card to finish sample adding; the incubation reaction disc device 9 is used for reacting the reagent card added with the sample, and sending the reacted reagent card to the detection device 10; in this embodiment, the control system 11 adopts a conventional numerical control system, and any reasonable numerical control system and suitable connection mode can be selected as required; under the control of the system control module, the sample feeding device 2, the shaking device 3, the sample feeding device 4, the buffer reaction device 5, the kit fixing device 7, the reagent card loading moving device 8, the incubation reaction disc device 9, the detection device 10 and the system control module are organically combined together, and the devices are mutually matched to jointly realize the purpose of automatically carrying out immune quantitative analysis. The sample to be detected is placed on the sample introduction platform 2-1 by an operator, and the detection result is directly finished without any operation. In addition, when the reagent cartridge is replaced, the used reagent cartridge is directly taken out and a new reagent cartridge is inserted without any complicated operation such as opening the apparatus. The internal structure of the fluorescence quantitative analyzer is deeply designed from installation, debugging and test stroke, so that the detection time of a project is shortened, and the stability of equipment is improved.

Referring to fig. 1, 5, 6 and 7, the sample injection device 2 comprises a sample injection platform 2-1; the sample introduction platform 2-1 comprises a to-be-detected area 2-1-1, a sampling area 2-1-2, a detected area 2-1-3 and an emergency treatment area 2-1-4; the sample feeding platform 2-1 is also provided with a first sample feeding motion mechanism 2-2, a second sample feeding motion mechanism 2-3, a third sample feeding motion mechanism 2-4 and a fourth sample feeding motion mechanism 2-5; the first sample introduction movement mechanism 2-2 pushes the test tube rack to move to the area to be detected 2-1-1; the second sample introduction movement mechanism 2-3 pushes the test tube rack to move the sampling area 2-1-2 from the area to be detected 2-1-1, and pushes the test tube rack after sampling to the area to be detected 2-1-3 continuously; the third sample feeding movement mechanism 2-4 pushes the test tube rack to push the tested area 2-1-3 out of the tested area 2-1-3; the fourth sample feeding and discharging motion mechanism 2-5 is used for sample feeding and discharging in the emergency treatment area 2-1-4; in the embodiment, the first sample injection motion mechanism 2-2, the second sample injection motion mechanism 2-3, the third sample injection motion mechanism 2-4 and the fourth sample injection motion mechanism 2-5 all adopt conventional linear motion mechanisms, and any reasonable structure and suitable connection mode can be selected according to requirements; for example, in this embodiment, the first sample feeding motion includes a motor, a synchronous belt, a U-shaped frame, a sliding frame, and a guide rail; the synchronous belt is arranged on one side of the U-shaped frame; the guide rail is arranged on the other side of the U-shaped frame; the motor drives the synchronous belt to move; one side of the sliding frame is connected with the guide rail; the other side of the sliding frame is connected with a synchronous belt; the synchronous belt is driven to move by the forward rotation of the motor, so that the sliding frame can be driven to move on the guide rail, and the sliding frame pushes the test tube rack to move forwards along the direction of the guide rail; when the motor rotates reversely, the sliding frame moves backwards along the direction of the guide rail to reset. The second sample feeding motion mechanism 2-3, the third sample feeding motion mechanism 2-4 and the fourth sample feeding motion mechanism 2-5 are the same. The emergency position in the emergency treatment area 2-1-4 is a sampling position when a detection result is required to be obtained urgently under special conditions, the emergency position is moved out of the equipment through a fourth sample introduction movement mechanism 2-5, a sample is placed on the emergency position, and then the sample is sent into the equipment to complete detection.

Referring to fig. 1 and 8, a shaking-up device 3 is further arranged on the whole machine bottom plate 1; the shaking device 3 is arranged in front of the sample feeding device 2 and used for shaking the test tube sample on the sampling position before sample feeding; the shaking device 3 comprises a rotating component 3-1, an up-down moving mechanism 3-2, a front-back moving mechanism 3-3 and a mounting plate 3-4; the mounting plates 3-4 are fixed on the whole machine bottom plate 1; the up-down moving mechanism 3-2 is fixed on the mounting plate 3-4; the front-back moving mechanism 3-3 is fixed on a sliding block of the up-down moving mechanism 3-2; the rotating component 3-1 is fixed on a sliding block of the front-back moving mechanism 3-3; the rotating assembly 3-1 is also provided with a test tube rack clamping hand 3-1-1; in this embodiment, the rotating assembly 3-1 may adopt a conventional motor-driven selection structure; the up-down moving mechanism 3-2 and the front-back moving mechanism 3-3 can adopt a conventional linear moving structure, and any reasonable structure and a proper connecting and installing mode can be selected according to requirements; for example, in the embodiment, the up-and-down movement comprises a motor, a screw rod and a connecting plate; the motor drives the screw rod to rotate; the connecting block is movably sleeved on the screw rod, and when the motor drives the screw rod to rotate, the screw rod drives the rotating assembly 3-1 fixed on the connecting plate to reciprocate along the screw rod; the front-back moving mechanism 3-3 is similar, thereby being beneficial to realizing the vertical movement of the test tube rack clamping hand 3-1-1 of the rotating component 3-1 in the Z-axis direction, the front-back movement in the Y-axis direction and the rotation around the Y-axis.

Referring to fig. 1 and 9, the sample adding device 4 comprises a sample sucking needle part 4-1 and a needle arm supporting component 4-2; the sample sucking needle part 4-1 is provided with a sample sucking needle 4-1-1; the sample sucking needle part 4-1 is used for driving the sample sucking needle 4-1-1 to suck samples on the test tube rack; the needle arm supporting component 4-2 is connected with the sample sucking needle 4-1-1; the needle arm supporting component 4-2 is used for driving the sample sucking needle 4-1-1 to move back and forth between the incubation reaction disc and the washing device 6;

the sample sucking needle part 4-1 enables the sample sucking needle 4-1-1 to move on the Z axis, and punctures a test tube provided with a sample on a test tube rack to suck the sample; the needle arm supporting component 4-2 drives the sample sucking needle part 4-1 to move on the Y axis and move to the buffer reaction device 5 to dilute and mix the sample; after dilution and uniform mixing, the needle arm supporting component 4-2 drives the sample sucking needle part 4-1 to move continuously on the Y axis, and the sample sucking needle part moves to a sample adding hole 9-1-1 of the incubation reaction disc device 9 for sample adding; after the sample adding is finished, the needle arm supporting component 4-2 drives the sample sucking needle part 4-1 to move back on the Y axis, the sample sucking needle part is moved into a washing station 6-1 of a washing device 6 to be washed, the washing is finished, and then the next sample adding is carried out. The process is as follows: absorbing a sample, diluting and uniformly mixing the sample in a buffer reaction device 5, adding the sample in an incubation reaction disc device 9, and finally cleaning the sample in a washing station 6-1 of a washing device 6 to finish one-time sample addition; and repeating the steps to finish the sample adding of other samples.

Referring to FIG. 12, the washing device 6 comprises a washing station 6-1, a first diaphragm pump 6-2, a second diaphragm pump 6-3, an injector 6-4, a first solenoid valve 6-5, a second solenoid valve 6-6, a cleaning solution tank 6-7, a pure water tank 6-8 and a waste water tank 6-9; the first diaphragm pump 6-2 is respectively connected to a cleaning liquid tank 6-7 and a pure water tank 6-8 through a first electromagnetic valve 6-5; the second electromagnetic valve 6-6 is arranged between the injector 6-4 and the first diaphragm pump 6-2; the second solenoid valve 6-6 is also connected to the washing station 6-1; one end of the second diaphragm pump 6-3 is connected with the washing station 6-1, and the other end is connected with the waste water tank 6-9; in this embodiment, the first diaphragm pump 6-2 functions to pump the cleaning water in the cleaning liquid tank 6-7 or the pure water in the pure water tank 6-8 to clean the pipette 4-1-1, and the second diaphragm pump 6-3 functions to pump the waste liquid from the washing station 6-1. And (5) uniformly mixing the buffer solution and the sample under the action of the injector 6-4, sucking, discharging for 3-5 times, and uniformly mixing the buffer solution and the sample. The action of the first electromagnetic valves 6-51 controls the on-off of the two liquid pipelines of the cleaning liquid and the pure water. The second electromagnetic valve 6-6 controls the on-off of the two liquid pipelines of the cleaning sample suction needle 4-1-1; in this embodiment, the washing station 6-1 is connected to the first diaphragm pump 6-2, the second diaphragm pump 6-3, the injector 6-4, the first solenoid valve 6-5, the second solenoid valve 6-6, the cleaning solution tank 6-7, the pure water tank 6-8, the waste solution tank, and the sample adding device 4 through a conventional liquid pipeline.

Referring to fig. 1, 10 and 11, the buffer reaction device 5 comprises a buffer solution part 5-1 and a mixing box part 5-2; the buffer liquid part 5-1 comprises a buffer liquid bottle 5-1-1 and a first driving mechanism 5-1-2; the first driving mechanism 5-1-2 is used for transferring the corresponding buffer liquid bottle 5-1-1 to the lower part of the sample sucking needle 4-1-1; the blending box part 5-2 comprises a blending box 5-2-1 and a second driving mechanism 5-2-2; the second driving mechanism 5-2-2 is used for transferring the blending box 5-2-1 to be used to the lower part of the sample adding needle; the buffer solutions with different pH values are filled in different buffer solution bottles 5-1-1, when one of the buffer solutions with different pH values is needed in the sample adding process, the first driving mechanism 5-1-2 moves the corresponding buffer solution to the position right below the Y-axis moving area of the sample adding needle; in the sample adding process, the second driving mechanism 5-2-2 enables the unused blending box 5-2-1 to be positioned right below the moving area of the sample adding needle in the Y axis; in this embodiment, the first driving mechanism 5-1-2 and the second driving mechanism 5-2-2 both adopt linear motion driving devices, for example, in this embodiment, the first driving mechanism 5-1-2 includes a motor driving part and a sliding part; the motor driving part comprises a motor, a belt and a belt fixing part; the motor is arranged at one end; the belt fixing part is arranged at the other end; the belt is connected with the output shaft of the stepping motor and the belt fixing part; the sliding part comprises a shaft seat, an optical axis and a sliding block; the shaft seat is fixed at one end; the optical axis is connected with the shaft seat; the sliding block is slidably sleeved in the optical axis and is fixed on the belt; corotation and the reversal of step motor are favorable to driving advancing and returning back of belt respectively to be favorable to driving advancing and returning back of the buffer solution bottle of being connected with the belt, be favorable to realizing the automatic transportation operation of buffer solution bottle, slider and belt fixed connection, when the belt transportation, can drive the slider and remove on the optical axis, be favorable to improving the stability that the slider slided. The second driving mechanism 5-2-2 has the same principle.

Referring to fig. 2 and 13, the reagent kit fixing device 7 comprises a reagent kit cartridge part 7-1, a micro-motion sensor 7-2 and an adsorption part 7-3; the reagent box card bin part 7-1 is embedded in the bottom plate 1 of the whole machine; a plurality of card bins are arranged on the reagent box card bin part 7-1, and a card bin outlet opening 7-1-1 is arranged at the bottom of each card bin; the micro-motion sensor 7-2 is fixed on one side surface of the upper reagent box card chamber part 7-1; each card bin corresponds to a micro-motion sensor 7-2; the magnet is arranged on one side surface of the reagent box card chamber part 7-1; each card bin corresponds to two adsorption pieces 7-3; in the embodiment, the card bin ports for inserting the reagent boxes are arranged outside the equipment, and the card bin outlet ports 7-1-1 are arranged at the bottom of each card bin, so that the reagent boxes can be directly replaced when needed. The micro sensor 7-2 is fixed on the back of the card bin opening of the card bin of the reagent kit, and the function of the micro sensor 7-2 is to identify whether the reagent kit is in the card bin; each card bin corresponds to two adsorption pieces 7-3, and the adsorption pieces 7-3 adopt magnets purchased in the conventional market and can attract corresponding magnets in the kit, so that the kit can be fixed in the card bins.

Referring to fig. 2, 3, 14 and 15, the reagent card loading moving device 8 includes an X-axis moving device 8-1 and a Y-axis moving device 8-2; the X-axis moving device 8-1 is fixed on a sliding block of the Y-axis moving device 8-2; a reagent card moving channel 8-1-2 is arranged on the X-axis moving device 8-1; the card inlet of the reagent card moving channel 8-1-2 is aligned with the card bin outlet 7-1-1 of the reagent box fixing device 7 under the driving of the Y-axis moving device 8-2; a hook and clamp moving part 8-1-1 is also arranged on the X axis; after the X-axis moving device 8-1 is aligned with the card bin outlet bayonet 7-1-1 of the kit card bin, a hook card moving part 8-1-1 on the X axis hooks out a reagent card from a card bin outlet bayonet 7-1-1 of a kit card bin, and the reagent card is hooked into the reagent card moving channel 8-1-2 of the X-axis moving device 8-1, after the hooking is finished, the Y-axis moving device 8-2 drives the X-axis moving device 8-1 to move, so that a bayonet outlet of a reagent card moving channel 8-1-2 on the X-axis moving device 8-1 is aligned with a reagent card inlet of the incubation reaction disc device 9, and a hooking moving part in the X-axis moving device 8-1 moves to the back of a reagent card in the reagent card moving channel 8-1-2 to push the reagent card to enter the incubation reaction disc device 9.

Referring to fig. 18, 19 and 20, the incubation reaction disc device 9 comprises a heat preservation shell 9-1, a rotary disc 9-2 and a rotary disc driving mechanism 9-3; the turntable driving mechanism 9-3 drives the turntable 9-2 to rotate in the heat-insulating shell 9-1; the turntable driving mechanism 9-3 is fixed on the detection device 10 and is vertically matched with the detection device 10; a sample adding hole 9-1-1 is formed in the heat preservation shell 9-1; in the embodiment, the heat-insulating shell 9-1 is made of conventional commercial products, and any reasonable material and structure of the heat-insulating shell 9-1 can be selected according to needs; the rotation driving mechanism rotates with the center of the rotary table 9-2, any reasonable rotation driving device and a proper connection installation mode can be selected according to needs, and the rotary table 9-2 can rotate in the heat preservation shell 9-1 conveniently.

Referring to fig. 18, 19 and 20, the detecting device 10 includes an optical path box 10-1, an optical path box supporting component 10-2 and a detecting driving mechanism 10-3; the light path box 10-1 is fixed on the light path box supporting component 10-2; the light path box supporting component 10-2 is provided with a light path reagent card moving channel 8-1-2; the light path reagent card moving channel 8-1-2 is butted with a card outlet of the incubation reaction disc device 9; the detection driving mechanism 10-3 moves the reagent card after the reaction from the incubation reaction disc device 9 to the optical path reagent card moving channel 8-1-2 of the optical path box supporting component 10-2, the optical path box 10-11 obtains the fluorescence value of the reaction in the moving process and transmits the fluorescence value to the control system 11, and the detection driving mechanism 10-3 moves the reagent card out of the device all the time to finish the detection.

The device modules complete the flow steps under the control of the full-automatic fluorescence quantitative analyzer control system 11, and the working flow is as follows:

putting a sample to be detected into a test tube, putting the test tube with the sample into a test tube rack matched with equipment, putting the test tube rack on a sample introduction platform 2-1, and starting the equipment. The control system 11 controls the first sample introduction movement mechanism 2-2 to push the test tube rack for fixing samples to move to the tail end of the to-be-detected area 2-1-1 on the sample introduction platform 2-1, a microswitch is triggered and feeds back signals to the control system 11, the control system 11 stops the movement of the first sample introduction movement mechanism 2-2, controls the second sample introduction movement mechanism 2-3 to continuously move the test tube rack for fixing samples to the sampling area 2-1-2 from the tail end of the to-be-detected area 2-1-1, the test tube rack moves to the scanning position of the bar code scanner of the sampling area 2-1-2 first, the bar code scanner arranged on the whole machine bottom plate 1 acquires bar code information on test tubes on the test tube rack and feeds back the bar code information to the control system 11, and the control system 11 sends instructions to the acquired bar code information.

There are two kinds of circumstances in the introduction sample area, and the first condition need shake even operation to the sample in the test tube, and the second condition need not shake even operation to the sample in the test tube.

If the sample to be detected needs to be shaken up, the control system 11 controls the second sample feeding motion mechanism 2-3 to move the sample to be detected to a sample feeding area shaking up position, the control system 11 sends an instruction to the shaking up device 3 to drive the shaking up device 3 to shake up the sample to be detected, after shaking up, placing a sample to be detected at an original position, driving the second sample introduction movement mechanism 2-3 again to move the sample to be detected to a sample position of a sample introduction area, controlling the sample introduction device 4 to drive by the control system 11, sucking quantitative gas firstly, sucking quantitative buffer solution secondly, sucking quantitative gas secondly, moving to the sample position to suck quantitative sample, moving to the blending box 5-2-1 to suck the buffer solution and the sample uniformly, sucking quantitative blending sample after blending, moving to the sample introduction hole 9-1-1, and introducing the blending sample into a corresponding reagent card in the incubation reaction disc device 9. The reagent card is subjected to constant temperature reaction in the incubation reaction plate device 9, after the reaction is finished, the detection driving device of the detection device 10 moves the reagent card into the detection reagent card moving channel 8-1-2 of the detection device 10, the light path box 10-1 of the detection device 10 obtains reaction information and feeds the information back to the control system 11, the control system 11 displays a result on equipment, and meanwhile, the detection driving device of the detection device 10 plays the reagent card which obtains the reaction information out of the equipment to finish the detection.

After the detection is finished, the sample adding device 4 is moved to the washing station 6-1 of the washing device 6 for cleaning, so that the sample is prevented from being polluted when the sample is sampled and added next time, and the accuracy of the detection result is reduced.

In the process of completing sampling and sample adding by the sampling device, the control system 11 simultaneously controls the buffer reaction device 5, the reagent card loading moving device 8 and the incubation reaction plate device 9, and completes the sample adding operation of the reagent card by matching with the sampling device.

And (3) the sampling device sucks a sample in the test tube to leave, the subsequent operation is completed until the cleaning is completed, in the period, the control system 11 controls the second sample introduction movement mechanism 2-3 to drive the test tube rack to move, the next sample to be detected is moved to a scanning position of a bar code scanner of the sampling area 2-1-2, the bar code scanner feeds information obtained by the information back to the control system 11, the control system 11 sends an instruction to complete whether the sample to be detected needs to be uniformly shaken or not, the sample to be detected is moved to the sampling area 2-1-2 after the completion, and the sampling device is waited to suck the sample. And shaking up the sample to be detected on the test tube rack according to the steps to obtain the sample.

When the samples in the test tubes on the test tube rack are detected, the control system 11 controls the second sample feeding movement mechanism 2-3 to move the test tube rack from the sampling area 2-1-2 to the tail end of the detected area 2-1-3, and the third sample feeding movement mechanism 2-4 moves the test tube rack with the fixed samples from the tail end of the detected area 2-1-3 to the top end of the detected area 2-1-3. And finishing the automatic sampling operation of the sample to be detected.

When the sample to be detected needs to obtain the detection result urgently, the sample to be detected is placed in the emergency treatment position in the emergency treatment area 2-1-4, the operation equipment stops all commands of the next detection sample, and the urgent detection sample is preferably executed. And manually operating the sample injection fourth movement mechanism to move the emergency position out of the equipment, placing the sample to be detected in emergency into the equipment, manually operating the fourth sample injection movement mechanism 2-5 to place the emergency position into the equipment, and waiting for the sample injection device 4 to absorb the sample to finish the detection.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the utility model can be made, and equivalents and modifications of some features of the utility model can be made without departing from the spirit and scope of the utility model.

Claims

1. A full-automatic fluorescence quantitative analyzer comprises a complete machine bottom plate and a control system; the method is characterized in that: the whole machine bottom plate is provided with a sample feeding device, a sample adding device, a buffer reaction device, a kit fixing device, a reagent card loading and moving device, an incubation reaction disc device and a detection device; the kit fixing device is used for providing a reagent card required by a detection item; the reagent card loading and moving device is used for obtaining a reagent card and transferring the reagent card to the incubation reaction disc device; the sample feeding device is used for providing a sample for the sample feeding device; the sample adding device transfers the sample to the buffer reaction device to be mixed uniformly, and the mixed sample is added into the corresponding reagent card to finish sample adding; the incubation reaction plate device is used for reacting the reagent card added with the sample and sending the reacted reagent card to the detection device.

2. The fully automatic fluorescence quantitative analyzer according to claim 1, characterized in that: the sample introduction device comprises a sample introduction platform; the sample introduction platform comprises a to-be-detected area, a sampling area, a detected area and an emergency treatment area; the sample feeding platform is also provided with a first sample feeding motion mechanism, a second sample feeding motion mechanism, a third sample feeding motion mechanism and a fourth sample feeding motion mechanism; the first sample feeding motion mechanism pushes the test tube rack to move to a to-be-detected area; the second sample introduction movement mechanism pushes the test tube rack to move from the area to be detected to the sampling area, and pushes the test tube rack after sampling to the detected area continuously; the third sample feeding movement mechanism pushes the test tube rack out of the detected area; and the fourth sample feeding motion mechanism is used for finishing detection when the emergency detection sample enters and exits the emergency treatment area.

3. The fully automatic fluorescence quantitative analyzer according to claim 1, characterized in that: the whole machine bottom plate is also provided with a shaking device; the shaking device is arranged in front of the sample feeding device and used for shaking the test tube sample on the sampling position before sample feeding; the shaking device comprises a rotating component, an up-and-down moving mechanism, a front-and-back moving mechanism and a mounting plate; the mounting plate is fixed on the whole machine bottom plate; the up-down moving mechanism is fixed on the mounting plate; the front-back moving mechanism is fixed on a sliding block of the up-down moving mechanism; the rotating assembly is fixed on a sliding block of the front-back moving mechanism; and the rotating assembly is also provided with a test tube rack clamping hand.

4. The fully automatic fluorescence quantitative analyzer according to claim 1, characterized in that: the sample adding device comprises a sample sucking needle part and a needle arm supporting component; the sample sucking needle part is provided with a sample sucking needle; the sample sucking needle part is used for driving a sample sucking needle to suck samples on the test tube rack; the needle arm supporting assembly is connected with the sample sucking needle; the needle arm supporting component is used for driving the sample suction needle to move back and forth between the incubation reaction disc and the washing device.

5. The fully automatic fluorescence quantitative analyzer according to claim 4, characterized in that: the washing device comprises a washing station, a first diaphragm pump, a second diaphragm pump, an injector, a first electromagnetic valve, a second electromagnetic valve, a cleaning liquid tank, a pure water tank and a waste water tank; the first diaphragm pump is respectively connected to the cleaning liquid tank and the pure water tank through a first electromagnetic valve; the second electromagnetic valve is arranged between the injector and the first diaphragm pump; the second solenoid valve is also connected to a washing station; one end of the second diaphragm pump is connected with the washing station, and the other end of the second diaphragm pump is connected with the waste water tank.

6. The fully automatic fluorescence quantitative analyzer according to claim 4, characterized in that: the buffer reaction device comprises a buffer solution part and a blending box part; the buffer part comprises a buffer liquid bottle and a first driving mechanism; the first driving mechanism is used for transferring the corresponding buffer liquid bottle to the lower part of the sample sucking needle; the blending box part comprises a blending box and a second driving mechanism; and the second driving mechanism is used for transferring the blending box to be used to the lower part of the sample adding needle.

7. The fully automatic fluorescence quantitative analyzer according to claim 1, characterized in that: the kit fixing device comprises a kit clamping cabin part, a micro-motion sensor and an adsorption part; the bottom of the reagent box clamping bin is embedded in the bottom plate of the whole machine; the reagent box card bin part is provided with a plurality of card bins, and the bottom of each card bin is provided with a card bin outlet; the micro-motion sensor is fixed on one side surface of the upper reagent box card chamber part; each card bin corresponds to a micro-motion sensor; the adsorption piece is arranged on one side surface of the cassette part of the reagent box; each card storehouse all corresponds two absorption pieces.

8. The fully automatic fluorescence quantitative analyzer according to claim 7, characterized in that: the reagent card loading and moving device comprises an X-axis moving device and a Y-axis moving device; the X-axis moving device is fixed on a sliding block of the Y-axis moving device; a reagent card moving channel is arranged on the X-axis moving device; the card inlet of the reagent card moving channel is driven by the Y-axis moving device to be aligned with the card outlet of the cartridge fixing device; and the X-axis moving device is also provided with a hook-and-clamp moving part.

9. The fully automatic fluorescence quantitative analyzer according to claim 1, characterized in that: the incubation reaction disc device comprises a heat preservation shell, a rotary disc and a rotary disc driving mechanism; the turntable driving mechanism drives the turntable to rotate in the heat-insulating shell; the turntable driving mechanism is fixed on the detection device and is vertically matched with the detection device; and the heat-insulating shell is provided with a sample adding hole.

10. The fully automatic fluorescence quantitative analyzer according to claim 1, characterized in that: the detection device comprises a light path box, a light path box supporting component and a detection driving mechanism; the light path box is fixed on the light path box supporting component; the light path box supporting component is provided with a light path reagent card moving channel; the light path reagent card moving channel is in butt joint with a card outlet of the incubation reaction disc device.