CN107356587A - A kind of instant detecting system of light-induced chemiluminescent - Google Patents

A kind of instant detecting system of light-induced chemiluminescent Download PDF

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Publication number
CN107356587A
CN107356587A CN201710738047.1A CN201710738047A CN107356587A CN 107356587 A CN107356587 A CN 107356587A CN 201710738047 A CN201710738047 A CN 201710738047A CN 107356587 A CN107356587 A CN 107356587A
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CN
China
Prior art keywords
kit
detecting system
light
reagent
platform
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710738047.1A
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Chinese (zh)
Other versions
CN107356587B (en
Inventor
杨全琪
张伟
包得泉
王宪华
徐辉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chengdu Aixing Biotechnology Co Ltd
BEIJING BEITAI TECHNOLOGY Co Ltd
Original Assignee
Chengdu Aixing Biotechnology Co Ltd
BEIJING BEITAI TECHNOLOGY Co Ltd
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Priority to CN201710738047.1A priority Critical patent/CN107356587B/en
Publication of CN107356587A publication Critical patent/CN107356587A/en
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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76Chemiluminescence; Bioluminescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326Magnetic particles

Abstract

The invention discloses a kind of instant detecting system of light-induced chemiluminescent, including:Kit, there are some reagent positions needed for experiment;Carry the reagent kit platform of the kit;Instant detecting system, including with the pipetting device for carrying out liquid transfer, and the testing agency to reaction system illuminated message collection in the kit.The present invention is not required to magnetic bead as carrier, avoids because magnetic bead loses or cleans the problem of Clean belt does not come, improves the accuracy of detection.

Description

A kind of instant detecting system of light-induced chemiluminescent
Technical field
The present invention relates to immune diagnostic technique field, and in particular to a kind of instant detecting system of light-induced chemiluminescent.
Background technology
Immunoassay is the important means of clinical diagnosis, and the immunoassay method of main flow is chemoluminescence method at present, is had The features such as sensitive, stable, it is widely used in clinical detection.Although for the immunoassay method before relatively, chemiluminescence is excellent Gesture is obvious, but it uses the coated system of magnetic bead mostly, has magnetic bead to clean link in experiment, can influence the accurate of experimental result Property.In addition, even the high-throughout detecting instrument of traditional method of testing also wants a few hours just to obtain testing result, and this Class experiment is typically all carried out under specific laboratory environment, the quick detection being not readily used for by field or bed.
The content of the invention
Therefore, the technical problem to be solved in the present invention is to overcome and of the prior art needs to utilize the coated detection of magnetic bead System, magnetic bead is not required to as carrier so as to provide one kind, avoids and carrys out otherness because magnetic bead loses or cleans not Clean belt Link is cleaned, improves the accuracy of detection.
A kind of instant detecting system of light-induced chemiluminescent of the present invention, including:
Kit, there are some reagent positions needed for experiment;
Instant detecting system, including with the pipetting device for carrying out liquid transfer, and, to reaction system in the kit The testing agency that illuminated message is collected.
Alternatively, the kit is disposable sealer kit.
Alternatively, the disposable sealer kit includes box body and the sealer on box body;The box body at least wraps Include sample bit, dilution position, reagent position, close smooth detecting position;The reagent position is provided with several.
Alternatively, the detecting system immediately also includes the reagent kit platform for carrying the kit.
Alternatively, the reagent kit platform has the reagent kit platform around central axis, along reagent kit platform week To provided with some kits, the kit bottom is provided with kit bracket, the adjacent kit tray chamber, and close examination The outer fringe position of agent kit platform is provided with pipetting head and places position, and pipetting head is set in the inner;The reagent kit platform is by reagent kit platform Motion module controls the reagent kit platform to rotate.
Alternatively, it is additionally provided with temperature control module on the reagent kit platform.
Alternatively, the pipetting device includes liquid relief component, liquid relief component water under the control of slide rail motion module Put down to linear motion, vertically to linear motion under the control of screw mandrel motion module;The liquid relief component include piston mechanism and With the stopper head of piston mechanism lower end elastic connection, the stopper head can be connected with the pipetting head.
Alternatively, the piston mechanism includes piston, the guide located at piston exterior and the motor for driving piston movement, Suction and transfer of the pipetting head to liquid are realized by piston movement.
Alternatively, the detector control module that the testing agency includes detector and control detector is moved;The inspection Surveying utensil has optical sensor.
Alternatively, the front end of the optical sensor adds camera lens.
Technical solution of the present invention, have the following advantages that:
The instant detecting system of a kind of light-induced chemiluminescent provided in an embodiment of the present invention, including with some needed for experiment The disposable sealer kit of reagent position and instant detecting system, instant detecting system are included with the liquid relief for carrying out liquid transfer Mechanism, and the testing agency to reaction system illuminated message collection in kit.Said structure avoids using magnetic bead and is used as suction The carrier with reference to various molecules is echoed, meanwhile, kit and pipetting head select disposable material, therefore, largely subtract Lack the cross pollution of test error and reagent and strain, improve the accuracy of experiment.
The instant detecting system of a kind of light-induced chemiluminescent provided in an embodiment of the present invention, uses disposable pipetting head and examination The carrier that agent box is shifted and stored as liquid, pipetting head is recyclable after experiment, reduces chemical contamination;Kit is set some Functional areas, the corresponding test box of a sample, do not increase extra consumptive material;Whole apparatus structure is compact, compact portable, is suitable to Site Detection.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, in describing below Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the dimensional structure diagram of the present invention;
Fig. 2 is the dimensional structure diagram of the present invention;
Fig. 3 is the structural representation of disposal reagent kit;
Fig. 4 is the structural representation of reagent kit platform of the present invention;
Fig. 5 is pipetting device structural representation of the present invention;
Fig. 6 is testing agency's structural representation of the present invention.
Embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation Example is part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
In the description of the invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ", The orientation or position relationship of the instruction such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to Be easy to the description present invention and simplify description, rather than instruction or imply signified device or element must have specific orientation, With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected;Can To be mechanical connection or electrical connection;Can be joined directly together, can also be indirectly connected by intermediary, Ke Yishi The connection of two element internals.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this Concrete meaning in invention.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each other It is be combined with each other into conflict can.
Embodiment 1
With reference to the accompanying drawings and examples, the present invention is described in detail.
A kind of instant detecting system of light-induced chemiluminescent of the present invention, including disposable sealer kit and chemiluminescence be When detecting system form.
Wherein, disposable sealer kit is shown in Figure 3, disposable sealer kit of the invention by kit 11 and Detection cup 15 forms;Kit 11 includes multiple functions and separates position, the present embodiment, including sample bit 112, dilution liquid level 113, examination Agent position 114 and close smooth detecting position 115, wherein, reagent position 124 has multiple, can be selected and Function Extension according to application, sample One's own department or unit includes but is not limited to a sample loading position, a sample samples position, sample loading position and sample position UNICOM, in sample bit With filter membrane, most of impurity in whole blood in sample loading position can be filtered, serum sample is taken by filter membrane into sample Sample position;Box body can capping film after the reaction reagent defined filling in advance and reaction cup;Preferably, have on kit 11 There is two-dimentional code bit 116, two-dimentional code bit 126 includes the information of kit 12, is easy to Quick Response Code testing agency 52 to obtain kit 12 Information;It is consumptive material to test the disposable sealer kit used, is changed with the completion of experiment.
Referring to shown in Fig. 1 and Fig. 2, the instant detecting system of chemiluminescence is right by the reagent kit platform 1 including carrying kit The testing agency 4 of reaction system illuminated message forms in the pipetting device 3 of liquid transfer, and collection kit.
Shown in Figure 4, reagent kit platform 1 is rotatable disc structure, and reagent kit platform 1 includes a kit bracket 13, kit bracket 13 can be matched with kit, kit is placed on kit bracket 13, and the present embodiment platform can be held Idling moves 12 kits 11, and kit 11 can be picked and placeed freely from reagent kit platform;Reagent kit platform 1 is also incubated with offer The temperature control module and reagent kit platform motion module 14 of reaction temperature are educated, reagent kit platform motion module 14 can control kit 11 rotate on disk, complete liquid relief and mix to operate with the cooperation of pipetting device 3, coordinate completion kit anti-with testing agency 4 Answer the detection of system;Between adjacent kit bracket 13, and pipetting head placement position is provided with close to the outer fringe position of reagent kit platform, Pipetting head 12 is set in the inner.
It is shown in Figure 5, pipetting device 2 at least from liquid relief component, motion platform and control liquid relief component level to or hang down Directly formed to the slide rail control module 21 and screw mandrel motion module 22 of linear motion, the present embodiment can also include removing pipetting head Module and pipetting head recycling bin;Two dimensional motion is done in the plane that motion platform at least can be vertical with reagent kit platform, can also It is made up of three-dimensional motion mechanism.Liquid relief component can be fixed on the moving platform, and can carry out two dimension or three maintenance and operations with motion platform Dynamic, liquid relief component includes the stopper head with the piston mechanism of its internal combustion and with piston mechanism lower end elastic connection, its bottom It can be connected with disposable pipetting head.Piston mechanism 3 is made up of piston rod and cavity, the guide of piston exterior, piston rod It can be moved up and down by motor driven, expansion or compressed air, coordinate the pipetting head of liquid relief component connection, liquid relief can be completed Operation.Specifically, pipetting device 2 is made up of liquid relief component, screw mandrel motion module 22 and slide rail motion module 21, piston mechanism 3 It is connected with screw mandrel motion module 22, piston mechanism 3 can be dropped on reagent kit platform under the control of screw mandrel motion module 22 Pipetting head 12 in, be fitted close by the top of rigid connection first 33 and pipetting head 12, rigid connection first 33 can ensure to tie The air-tightness of structure.Pipetting head 12 drives pipetting head 12 to carry out the linear motion in vertical direction by screw mandrel motion module 22.Silk Bar motion module 22 is connected with slide rail motion module 21, and screw mandrel motion module 22, can be with the control of slide rail motion module 21 Move in the horizontal direction.
Piston mechanism 3 is made up of piston 32 and piston movement control module 31, and the one end of piston 32 inserts in rigid connection first 33 Inside, it is connected by the sealing ring positioned at first 33 top of rigid connection with rigid connection first 33 and keeps air-tightness.Piston 32 is another One end connection piston movement control module 31, is controlled by it the linear motion of piston 32 in vertical direction.In embodiment, piston The connection control piston 32 of motion-control module 31.
Shown in Figure 6, testing agency 4 is made up of light path, light path motion and close light shield mechanism, light path At least it is made up of excitation source 42 and detector 43, excitation source 42 exposes to reaction system, and reaction system produces light activation Luminescence-producing reaction, detector 43 collect the fluorescence of reaction transmitting, and detector motion control module 41 can control excitation source 42 Moved in vertical plane with detector 43, the motion of reagents kit platform motion module 14 can be pointed to reagent kit platform 12 kits 11 on 1 carry out close light detection successively.In this implementation, excitation source 42 is typically put with detector 43 in 90 degree Put, light source, with that during reagent disc platform routing motion the reaction system in detection cup can be made to receive illumination, produces with detector Light-induced chemiluminescent reacts, and detector reads signal.
Preferably, camera lens can be placed before excitation source 42, for effectively being excited to reaction system in detection cup, detect Camera lens 44 can be placed before device 43, for more effectively being collected to the reaction system in detecting position 115 is luminous.
When obtaining pipetting head 12, reagent kit platform 1 drives pipetting head 12 to move, and rigid connection first 33 is by pipetting device 2 Slide rail motion module 21 be moved to the vertical direction of pipetting head 12, afterwards by under the screw mandrel motion module 22 in pipetting device 2 It is down in the pipetting head 12 on the first 33 insertion reagent kit platform 1 of rigid connection, in the screw mandrel motion module 22 in pipetting device 2 Rise, depart from reagent kit platform 1, obtain pipetting head 12.
When carrying out liquid transfer, reagent kit platform 1 drives kit 11 to move, and is moved to immediately below pipetting head 12, liquid relief The first 12 slide rail motion module 21 in pipetting device 2, which is moved to kit 11, needs the hole position of liquid relief, and pipetting head 12 is in liquid relief Enter under the drive of screw mandrel motion module 22 in mechanism 2 in the corresponding hole site liquid of kit 11 for needing liquid relief, now piston 32 move upwards under the manipulation of piston movement control module 31, liquid is entered in pipetting head 12, afterwards the silk in pipetting device 2 Bar motion module 22 is moved up, and drives pipetting head 11 to leave kit 11, and the slide rail motion module 21 in pipetting device 2, which is moved to, to be needed Carry the corresponding hole site in the kit 11 of liquid, the drive of screw mandrel motion module 22 of the pipetting head 12 in pipetting device 2 Lower to enter in the relevant position of kit 11 of carrying liquid, piston 32 moves downward under the manipulation of piston movement control module 31, The liquid in pipetting head 11 is drained into target location, complete the transfer of liquid.
Kit 11 pre-installs the disposable pipetting head 12 of special requirement, can be by first 33 institute of rigid connection on pipetting device Obtain, trash receptacle can be positioned over after the completion of experiment by pipetting device and carry out waste recovery.
Chemiluminescence detection system may also include LCDs and printer, and LCDs can carry out Setup Experiments And result is shown, printer can carry out result printing.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or Among changing still in the protection domain of the invention.

Claims (10)

  1. A kind of 1. instant detecting system of light-induced chemiluminescent, it is characterised in that including:
    Kit, there are some reagent positions needed for experiment;
    Instant detecting system, including with the pipetting device for carrying out liquid transfer, and, reaction system in the kit is lighted The testing agency of information.
  2. 2. the instant detecting system of light-induced chemiluminescent according to claim 1, it is characterised in that the kit is once Property sealer kit.
  3. 3. the instant detecting system of light-induced chemiluminescent according to claim 2, it is characterised in that the disposable sealer examination Agent box includes box body and the sealer on box body;The box body comprises at least sample bit, dilution position, reagent position, the detection of close light Position;The reagent position is provided with several.
  4. 4. the instant detecting system of light-induced chemiluminescent according to claim any one of 1-3, it is characterised in that described to be When detecting system also include the reagent kit platform that carries the kit.
  5. 5. the instant detecting system of light-induced chemiluminescent according to claim 4, it is characterised in that the reagent kit platform tool There is the reagent kit platform around central axis, some kits, the kit bottom are circumferentially with along the reagent kit platform Provided with kit bracket, the adjacent kit tray chamber, and put close to the outer fringe position of reagent kit platform provided with pipetting head Set, pipetting head are set in the inner;The reagent kit platform controls the reagent kit platform to rotate by reagent kit platform motion module.
  6. 6. the instant detecting system of light-induced chemiluminescent according to claim 5, it is characterised in that on the reagent kit platform It is additionally provided with temperature control module.
  7. 7. the instant detecting system of light-induced chemiluminescent according to claim any one of 1-6, it is characterised in that the shifting Liquid mechanism includes liquid relief component, and liquid relief component level under the control of slide rail motion module to linear motion, is transported in screw mandrel Vertically to linear motion under the control of dynamic model block;The liquid relief component is elastic including piston mechanism and with the piston mechanism lower end The stopper head of connection, the stopper head can be connected with the pipetting head.
  8. 8. the instant detecting system of light-induced chemiluminescent according to claim 7, it is characterised in that the piston mechanism includes Piston, the guide located at piston exterior and the motor for driving piston movement, realize the pipetting head to liquid by piston movement The suction and transfer of body.
  9. 9. the instant detecting system of light-induced chemiluminescent according to claim 1, it is characterised in that the testing agency includes Detector and the detector control module of control detector motion;The detector has optical sensor.
  10. 10. the instant detecting system of light-induced chemiluminescent according to claim 9, it is characterised in that the optical sensor Front end adds camera lens.
CN201710738047.1A 2017-08-24 2017-08-24 Light-activated chemiluminescence instant detection system Active CN107356587B (en)

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Cited By (8)

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CN108303556A (en) * 2018-01-10 2018-07-20 中南林业科技大学 A kind of medicinal intelligent biochemical detection system
WO2019154348A1 (en) * 2018-02-11 2019-08-15 北京科美生物技术有限公司 Fully automatic photoexcited chemiluminescence detector
CN110412019A (en) * 2018-04-28 2019-11-05 上海索昕生物科技有限公司 A kind of detection device and full-automatic light-induced chemiluminescent detector
WO2020042596A1 (en) * 2018-09-02 2020-03-05 广州源起健康科技有限公司 Fully-automatic magnetic bead time-resolved fluoroimmunoassay instrument
CN111323411A (en) * 2020-03-21 2020-06-23 博源诺信(北京)生物科技有限责任公司 Multi-project combined chemiluminescence instant detection system
CN111413328A (en) * 2020-04-28 2020-07-14 上海泰辉生物科技有限公司 Dual-mode instant detection system
CN111537757A (en) * 2019-06-19 2020-08-14 丹娜(天津)生物科技股份有限公司 Biochemical analyzer
CN113841054A (en) * 2020-04-20 2021-12-24 利奥碧欧株式会社 Glycated hemoglobin measurement apparatus and method

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CN108303556A (en) * 2018-01-10 2018-07-20 中南林业科技大学 A kind of medicinal intelligent biochemical detection system
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WO2020042596A1 (en) * 2018-09-02 2020-03-05 广州源起健康科技有限公司 Fully-automatic magnetic bead time-resolved fluoroimmunoassay instrument
CN111537757A (en) * 2019-06-19 2020-08-14 丹娜(天津)生物科技股份有限公司 Biochemical analyzer
CN111323411A (en) * 2020-03-21 2020-06-23 博源诺信(北京)生物科技有限责任公司 Multi-project combined chemiluminescence instant detection system
CN113841054A (en) * 2020-04-20 2021-12-24 利奥碧欧株式会社 Glycated hemoglobin measurement apparatus and method
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CN111413328A (en) * 2020-04-28 2020-07-14 上海泰辉生物科技有限公司 Dual-mode instant detection system
CN111413328B (en) * 2020-04-28 2023-03-14 上海泰辉生物科技有限公司 Dual-mode instant detection system

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