CN114200123B

CN114200123B - Chemiluminescent immunoassay analyzer

Info

Publication number: CN114200123B
Application number: CN202111486740.7A
Authority: CN
Inventors: 叶翔; 谭运攀
Original assignee: Shenzhen Shixin Medical Technology Co ltd
Current assignee: Shenzhen Shixin Medical Technology Co ltd
Priority date: 2021-12-07
Filing date: 2021-12-07
Publication date: 2024-08-16
Anticipated expiration: 2041-12-07
Also published as: CN114200123A

Abstract

The application belongs to the technical field of detection equipment, and provides a chemiluminescent immunoassay analyzer which comprises a control system arranged on a rack, a reaction cup feeding and conveying system, a mixing system, a collecting system, an incubation and cleaning system and a light measuring system, wherein the reaction cup feeding and conveying system, the mixing system, the collecting system, the incubation and cleaning system and the light measuring system are arranged on the rack and are respectively electrically connected with the control system, the reaction cup feeding and conveying system is used for storing, feeding and carrying reaction cups, the collecting system is used for transferring samples in sample tubes and reagents in reagent tubes to mixing positions and collecting information of the sample tubes and the reagent tubes, the mixing system is used for mixing substances in the reaction cups, the incubation and cleaning system is used for incubating, cleaning, adding substrates and mixing the substances in the reaction cups, and the light measuring system is used for optically detecting the substances in the reaction cups after the incubation, the cleaning, the substrate adding and the mixing operations. The chemiluminescent immunoassay analyzer provided by the application has high automation degree, and can avoid the influence of human factors on the accuracy and reliability of detection as much as possible.

Description

Chemiluminescent immunoassay analyzer

Technical Field

The application relates to the technical field of detection equipment, in particular to a chemiluminescent immunoassay analyzer.

Background

Chemiluminescent immunoassay is a novel ultramicro analytical technique combining a chemiluminescent assay technique with high sensitivity with a highly specific immune response.

At present, most of chemiluminescent immunoassay analyzers in the industry adopt a manual test operation and semi-automatic detection mode, the detection instrument only usually realizes a detection reading function, uncertainty such as individual difference, manual error and operation non-standardization exists in manual operation, defects are exposed and not left under the condition of a large number of samples and test items, the problem of low automation degree exists, and the detection accuracy and reliability are affected.

Disclosure of Invention

The embodiment of the application aims to provide a chemiluminescent immunoassay analyzer, which aims to solve the technical problems that the chemiluminescent immunoassay analyzer in the prior art is low in automation degree and affects the accuracy and reliability of detection.

In order to achieve the above purpose, the application adopts the following technical scheme: the chemiluminescent immunoassay analyzer comprises a frame, a reaction cup feeding and conveying system, a mixing system, a collecting system, an incubation and cleaning system, a photometry system and a control system, wherein the reaction cup feeding and conveying system, the mixing system, the collecting system, the incubation and cleaning system, the photometry system and the control system are arranged on the frame;

The reaction cup feeding and conveying system comprises a cup feeding mechanism for storing and feeding reaction cups and a carrying mechanism which is movably arranged on the stand and is used for carrying the reaction cups;

the mixing system is provided with a mixing position and is used for mixing substances in the reaction cup at the mixing position uniformly;

The collection system comprises a sample injection mechanism which is used for storing and dispatching a sample tube and is provided with a sample suction position and an information collection position, a first information collection mechanism which is rotatably arranged on the rack and is used for storing and dispatching a reagent tube and is provided with a reagent suction position and a reagent disk mechanism, a transfer mechanism which is movably arranged on the rack and is used for transferring samples in the sample tube of the sample suction position and reagents in the reagent tube of the reagent suction position to the reaction cup on the mixing position, a rotation mechanism which is used for driving the sample tube on the information collection position to rotate, a first information collection mechanism which is used for collecting information of the sample tube rotating on the information collection position, and a second information collection mechanism which is used for collecting information of the reagent tube in the reagent disk mechanism;

the incubation and cleaning system is used for performing incubation operation on substances in the reaction cup which are uniformly mixed by the uniform mixing system, and cleaning operation, substrate filling operation and uniform mixing operation on the substances in the reaction cup which are subjected to incubation operation;

the photometry system comprises a photometry mechanism which is arranged at one side of the incubation cleaning system and is used for carrying out optical detection on substances in the reaction cup passing through the incubation cleaning system;

The control system is respectively and electrically connected with the reaction cup feeding and conveying system, the mixing system, the collecting system, the incubation and cleaning system and the photometry system.

In one embodiment, the transfer mechanism includes a sample needle, a sample arm, a sample adding driver, a reagent needle, a reagent arm, and a reagent adding driver, wherein the sample needle is disposed at one end of the sample arm, the sample adding driver is used for driving the sample arm to move so that the sample needle moves to the sample sucking position or the mixing position, the reagent needle is disposed at one end of the reagent arm, and the reagent adding driver is used for driving the reagent arm to move so that the reagent needle moves to the reagent sucking position or the mixing position.

In one embodiment, the transfer mechanism further comprises a first cleaner for cleaning the sample needle and a second cleaner for cleaning the reagent needle.

In one embodiment, the rotating mechanism comprises a first shaft, a second shaft, a rotating wheel, a pre-pressing arm, two pre-pressing wheels and a position adjusting assembly, wherein the first shaft and the second shaft are arranged on two sides of the information acquisition position; the rotating wheel is rotatably arranged on the first shaft and used for abutting against the outer surface of the sample tube positioned at the information acquisition position so as to rotate the sample tube; the pre-pressing arm is rotatably sleeved on the second shaft, the two pre-pressing wheels are arranged on the pre-pressing arm, the position adjusting assembly is used for applying an elastic force to the pre-pressing arm, so that the pre-pressing arm drives the two pre-pressing wheels to elastically press the outer surface of the sample tube at the information acquisition position.

In one embodiment, the position adjusting assembly comprises a mounting seat, a guide rod and an elastic piece, wherein the mounting seat is provided with the second shaft, and the mounting seat is further provided with a first limiting surface; the first end of the pre-pressing arm is connected with two pre-pressing wheels, and the second end of the pre-pressing arm is provided with a sliding groove; the first end of the guide rod is in sliding fit with the chute, and the second end of the guide rod is fixed on the mounting seat; the elastic piece is sleeved on the guide rod and is abutted between the mounting seat and the pre-pressing arm, and after the sample tube leaves the information acquisition position, the pre-pressing arm is abutted to the first limiting surface under the action of the elastic piece.

In one embodiment, the incubation cleaning system comprises a pot body, a cleaning disc, an incubation driver, a cleaning driver and a cleaning module, wherein the pot body is arranged on the rack, the incubation disc and the cleaning disc are coaxially arranged in the pot body, the incubation disc is positioned above the cleaning disc, the inner diameter of the incubation disc is smaller than that of the cleaning disc, an incubation groove for accommodating the reaction cup is formed in the upper portion of the incubation disc, a cleaning groove for accommodating the reaction cup is formed in the part of the cleaning disc, which exceeds the incubation disc, the incubation driver is used for driving the incubation disc to rotate, and the cleaning driver is used for driving the cleaning disc to rotate so as to drive the reaction cup on the cleaning disc to perform cleaning operation, mixing operation and substrate filling operation on the cleaning module.

In one embodiment, the cleaning module comprises a first lifting plate, a plurality of liquid suction needles, a second lifting plate and liquid injection needles, wherein the liquid suction needles are arranged on the first lifting plate along the circumferential direction of the first lifting plate, the liquid injection needles are arranged on the second lifting plate along the circumferential direction of the second lifting plate and have the same quantity as the liquid suction needles, the liquid suction needles and the liquid injection needles are arranged alternately, the liquid suction level is arranged below the liquid suction needles, the liquid injection level is arranged below the liquid injection needles, and the cleaning disc drives the reaction cup to suck liquid at the liquid suction positions and drives the reaction cup to inject liquid at the liquid injection level when rotating.

In one embodiment, the incubation and cleaning system further comprises two substrate liquid filling pipes, wherein the two substrate liquid filling pipes are respectively used for filling substrate liquid required by direct chemiluminescence and substrate liquid required by enzymatic reaction chemiluminescence into the reaction cup subjected to the cleaning operation.

In one embodiment, the light measuring mechanism comprises a camera bellows, a shading driver, a shading component and a photomultiplier, wherein the camera bellows is provided with a containing cavity, a first light hole and a second light hole which are oppositely arranged are arranged on the cavity wall of the containing cavity, the shading driver is arranged on the camera bellows, and one end of an output shaft of the shading driver is arranged in the containing cavity; the photomultiplier install in first light trap department and pass through first light trap receives the light signal, shading subassembly set up in the inside of holding chamber, shading subassembly includes shading arm and swing arm, be equipped with the locating pin on the chamber wall of holding chamber, shading arm rotationally overlaps to locate on the locating pin, the one end of swing arm connect in shading driver's output shaft, just the other end of swing arm is used for driving shading arm is rotatory, so that shading arm shelters from the second light trap or follow second light trap department removes.

In one embodiment, the sample feeding mechanism comprises an emergency sample tube support and an emergency driver, the sample feeding mechanism is further provided with an emergency position, the emergency position is located on the movement track of the transfer mechanism, an emergency sample tube groove for accommodating the emergency sample tube is formed in the emergency sample tube support, and the emergency driver is used for driving the emergency sample tube support to move on the frame so that the emergency sample tube on the emergency sample tube enters or leaves the emergency position.

In one embodiment, the sample feeding mechanism further comprises a sample feeding sample tube support and a sample feeding driver, the information collecting position is located on the sample feeding side of the sample feeding position, the sample feeding sample tube support is provided with a plurality of sample tube grooves for accommodating the sample tubes, and the sample feeding driver is used for driving the sample feeding sample tube support to move on the frame so that each sample tube on the sample feeding sample tube support sequentially passes through the sample feeding position and each sample tube sequentially passes through the information collecting position.

In one embodiment, the cup feeding mechanism comprises a loading storage part arranged on the rack and used for loading and storing the reaction cup, a conveying part arranged on one side of the loading storage part and in butt joint with the loading storage part, and a rotating disc in butt joint with the conveying part.

In one embodiment, the chemiluminescent immunoassay analyzer further comprises a cup system comprising a waste recovery mechanism for recovering substances within the used reaction cup and a waste cup recovery mechanism for recovering the used reaction cup.

The chemiluminescent immunoassay analyzer provided by the application has the beneficial effects that: compared with the prior art, the chemiluminescent immunoassay analyzer provided by the embodiment has the advantages that the carrying mechanism in the reaction cup feeding and conveying system carries the reaction cup fed by the cup feeding mechanism to the mixing position of the mixing system, the first information acquisition mechanism of the acquisition system automatically acquires the information of the sample tube at the information acquisition position, the second information acquisition mechanism acquires the information of the reagent tube in the reagent disc mechanism, the sample in the sample tube at the sample suction position of the sample feeding mechanism and the reagent in the reagent suction position of the reagent disc mechanism are automatically transferred to the reaction cup at the mixing position, the mixing system carries the sample and the reagent in the reaction cup at the mixing position to the incubation and cleaning system, the incubation and cleaning system automatically completes the incubation and cleaning operation, the substrate filling operation and the mixing operation (when the reaction cup is required to be carried back to the mixing system, the mixing operation is carried back by the carrying mechanism), and the reaction cup in the incubation and cleaning system rotates to the position aligned with the light measuring system, the light measuring system automatically detects the sample in the reaction cup, and the whole process can be accurately detected by the light system, and the whole process can be prevented from being influenced by human factors.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments or the description of the prior art will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic top view of a chemiluminescent immunoassay analyzer according to an embodiment of the present application;

FIG. 2 is a schematic diagram of a chemiluminescent immunoassay analyzer according to an embodiment of the present application;

FIG. 3 is a schematic structural view of a reagent disk mechanism according to an embodiment of the present application;

fig. 4 is a schematic structural diagram of a sample injection mechanism, a rotation mechanism, and a first information acquisition mechanism according to an embodiment of the present application;

Fig. 5 is a schematic top view of an incubation and cleaning system according to an embodiment of the application;

FIG. 6 is a cross-sectional view taken in the A-A direction of the incubation and cleaning system of FIG. 5 with the photometry system removed;

FIG. 7 is a schematic view of a part of a rotating mechanism according to an embodiment of the present application;

FIG. 8 is a schematic top view of the rotary mechanism of FIG. 7;

FIG. 9 is a schematic diagram of a light measuring system according to an embodiment of the present application;

FIG. 10 is a cross-sectional view of the photometry system of FIG. 9;

FIG. 11 is a schematic diagram of a testing flow of a chemiluminescent immunoassay analyzer according to an embodiment of the present application;

wherein, each reference sign in the figure:

100-frames;

200-a reaction cup feeding and conveying system; 210-a cup feeding mechanism; e-cup feeding position; 211-a load store; 212-a transfer section; 213-rotating disk; 220-a handling mechanism;

300-uniformly mixing the components; a-mixing evenly;

400-acquisition system; 410-a sample injection mechanism; b-sample sucking position; c-information acquisition bits; f-emergency department; 411-sample tube holder; 412-a sample injection driver; 413—sample pre-detection placement area; 414-sample injection track; 415-post-sample detection placement area; 416-emergency sample tube holder; 417-emergency driver; 420-a reagent tray mechanism; d-reagent sucking site; 421-reagent tray; 4211-pinion gear; 422-a reagent pan; 423-a reagent disk drive; 424-turning means; 4241-a large gear; 430-a transfer mechanism; 431-sample needle; 432-sample arm; 433-sample adding driver; 434-a reagent needle; 435-a reagent arm; 436-reagent-adding driver; 437-first washer; 438-a second washer; 440-a rotation mechanism; 441-a first shaft; 442-second axis; 443-rotating a wheel; 444-pre-pressing wheel; 445-pre-pressing arm; 4451-a chute; 4452-groove; 446-a position adjustment assembly; 4461-mount; 4462-guide bar; 4463-elastic member; 4464-a first stop surface; 4465-a substrate; 4466-a platen; 4467-second limit surface; 4468-an adjustment wheel; 450-a first information acquisition mechanism; 460-a second information acquisition mechanism;

500-incubation and cleaning system; g-incubation site; h, cleaning the position; i-the level of suction; j-filling level; k-taking the cup position of the waste cup; 510-a pot body; 511-bottom of the pan; 512-cover; 513-mounting holes; 520-cleaning the tray; 530-incubation plate; 540-incubation driver; 550-cleaning the driver; 560-cleaning the module; 561-first lifter plate; 562-a pipette needle; 563-a second lifter plate; 564-a liquid injection needle; 570-rotation axis; 580-rotating sleeve; 590-substrate liquid filling tube;

600-photometry system; 610-camera bellows; 611-a receiving chamber; 612-first light holes; 613-second light holes; 614-locating pins; 620-a light shielding driver; 630-a shade assembly; 631-a shade arm; 632-swing arm; 640-photomultiplier tubes;

700-control system;

800-a cup throwing system; 810-a waste liquid recovery mechanism; 820-a waste cup recovery mechanism; m-cup throwing position.

Detailed Description

Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present application and should not be construed as limiting the application.

In the description of the present application, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

Referring to fig. 1, a chemiluminescent immunoassay analyzer according to an embodiment of the present application will now be described. The chemiluminescent immunoassay analyzer is mainly used for detecting and analyzing substances such as antigens, hapten, antibody, hormone, enzyme, fatty acid, vitamin, medicine and the like, wherein the adopted analysis method is a chemiluminescent immunoassay method, the chemiluminescent immunoassay method is a method combining immune reaction and chemiluminescence and mainly comprises direct chemiluminescent analysis and enzymatic chemiluminescent analysis, wherein the direct chemiluminescent analysis is an analysis method for marking luminescent substances on the substances such as the antigens or the antibodies and the like, adding corresponding substrate liquid into reaction liquid after waiting for the immune reaction is finished, so that the luminescent substances emit light, and then measuring the concentration of the antigens or the antibodies by measuring the luminous intensity; the enzymatic chemiluminescent assay is an assay method in which an enzyme is labeled on a substance such as an antigen or an antibody, and after completion of an immunological reaction, a corresponding substrate solution is added to the reaction solution to cause the substrate solution to emit light, and then the concentration of the antigen or antibody is measured by measuring the intensity of the emitted light.

The chemiluminescent immunoassay analyzer provided in this embodiment includes a rack 100, a cuvette feeding and conveying system 200, a mixing system 300, a collecting system 400, an incubation and cleaning system 500, a photometry system 600 and a control system 700, wherein the cuvette feeding and conveying system 200, the mixing system 300, the collecting system 400, the incubation and cleaning system 500 are arranged on the rack 100. The rack 100 is a supporting structure of the whole chemiluminescent immunoassay analyzer, and the control system 700 is electrically connected with the reaction cup feeding and conveying system 200, the mixing system 300, the collecting system 400, the incubation system and the photometry system 600 respectively, and is used for controlling the chemiluminescent immunoassay analyzer to automatically perform chemiluminescent immunoassay.

The cuvette feeding and conveying system 200 includes a cuvette feeding mechanism 210 and a handling mechanism 220, the cuvette feeding mechanism 210 is used for storing and feeding the cuvette, and the handling mechanism 220 is movably arranged on the frame 100 and is used for handling the cuvette fed by the cuvette feeding mechanism 210.

Mixing system 300 has a mixing location a and is used to mix the substances placed in the reaction cup at mixing location a.

The collection system 400 includes a sample introduction mechanism 410, a reagent tray mechanism 420, a transfer mechanism 430, a rotation mechanism 440, a first information collection mechanism 450, and a second information collection mechanism 460. The sample feeding mechanism 410 is used for storing and dispatching sample tubes and has a sample sucking position b and an information collecting position c, the reagent disk mechanism 420 is rotatably arranged on the rack 100 and is used for storing and dispatching reagent tubes and has a reagent sucking position d, the transfer mechanism 430 is movably arranged on the rack 100 and is used for transferring samples in the sample tubes sucking the sample position b and reagents in the reagent tubes sucking the reagent position d to a reaction cup on the mixing position a, the rotating mechanism 440 is used for driving the sample tubes on the information collecting position c to rotate, the first information collecting mechanism 450 is used for collecting information of the sample tubes rotating on the information collecting position c, and the second information collecting mechanism 460 is used for collecting information of the reagent tubes in the reagent disk mechanism 420.

The incubation and washing system 500 is used for performing an incubation operation on the substances in the reaction cup which are mixed by the mixing system 300, and for performing a washing operation, a substrate filling operation and a mixing operation on the substances in the reaction cup which are subjected to the incubation operation.

The photometry system 600 is disposed at one side of the incubation and washing system 500 for optically detecting substances in the reaction cup passing through the incubation and washing system 500.

The control system 700 is electrically connected to the cuvette feeding and transporting system 200, the mixing system 300, the collection system 400, the incubation and cleaning system 500, and the photometric system 600, respectively.

The information of the sample tube may be information such as a name of a patient to which the sample belongs, a name of an item to be detected, etc., the information of each reagent tube in the reagent tray mechanism 420 may be information of a position of the reagent tube in the reagent tray 421, information such as a name, a concentration, a volume, etc. of a reagent contained in the reagent tube, the second information collecting mechanism 460 may collect information of each reagent tube placed in the reagent tray mechanism 420 in real time, a plurality of positions for placing the reagent tube are divided in the reagent tray mechanism 420, and when each position for placing the reagent tube can only place a specific reagent corresponding to the position, the second information collecting mechanism 460 may replace the information of the reagent tube by collecting or storing the information of the specific reagent placed in each position in the reagent tray mechanism 420, and may be set as required, not be limited herein, and the control system 700 may use the information of the reagent tube and the information of the sample tube to match the corresponding reagent for the sample.

Compared with the prior art, the chemiluminescent immunoassay analyzer provided by the embodiment of the invention has the advantages that the carrying mechanism 220 in the reaction cup feeding and conveying system 200 carries the reaction cup fed by the cup feeding mechanism 210 to the mixing position a of the mixing system 300, the first information acquisition mechanism 450 of the acquisition system 400 acquires the information of the sample tube at the information acquisition position c, the second information acquisition mechanism 460 acquires the information of the reagent tube in the reagent tray mechanism 420, the sample in the sample tube at the sample suction position b of the sample feeding mechanism 410 and the reagent at the reagent suction position d of the reagent tray mechanism 420 are automatically transferred to the reaction cup at the mixing position a, the mixing system 300 carries the sample and the reagent in the reaction cup at the mixing position a to the incubation and cleaning system 500, the incubation and cleaning system 500 automatically completes the incubation operation, the cleaning operation, the substrate back-filling operation and the mixing operation (when the reaction cup is required to be carried back to the mixing system 300, the reaction cup is carried back to the mixing position a by the carrying mechanism 220), the whole-course automatic detection of the reaction cup in the whole course can be carried by the optical system 600, and the influence of the whole-course can be avoided by the automatic detection of the optical system 600 on the accuracy of detecting the quality of the reaction can be detected.

In another embodiment of the present application, referring to fig. 3, the reagent disk mechanism 420 includes a reagent disk 421 for carrying reagent tubes, a reagent pot 422 surrounding the reagent disk 421 and capable of refrigerating the reagent tubes carried in the reagent disk 421, a reagent disk driver 423 drivingly connected to the bottom side of the reagent disk 421 to drive the reagent disk to rotate, and a rotating device 424 disposed between the outer pot and the reagent disk 421 for driving the reagent tubes to rotate for mixing operation.

The reagent is added with magnetic beads which are particles with specific active groups on the surface, uniform size, sphericity, superparamagnetism and protective shell. The magnetic beads show magnetic force in the presence of an external magnetic field to gather, and lose the magnetic force to disperse in the absence of the magnetic field. The method comprises the steps of combining active substances such as antigen/antibody and the like with active groups on the surface of magnetic beads to coat the active substances on the surface of the magnetic beads, incubating the object to be detected and the magnetic beads coated with the antigen/antibody under certain conditions during detection, combining the active substances and the magnetic beads through antigen-antibody reaction, adding an external magnetic field to generate magnetism to gather the active substances and the magnetic beads, performing washing operation, separating an antigen-antibody combined part from an unbound part, adding substances such as substrate liquid and the like, detecting emitted optical signals by using a photometry system 600, and analyzing the content of the antigen/antibody in the object to be detected. The rotating device 424 provided in this embodiment is used to make the magnetic beads in the reagent uniformly distributed, so as to ensure the performance of the magnetic bead reagent.

Specifically, the rotation device 424 may be a large gear 4241, the large gear 4241 is fixed in the reagent pot 422, the reagent disk driver 423 is disposed at the bottom of the reagent pot 422, the reagent disk 421 is fixedly sleeved on the output shaft of the reagent disk driver 423 and coaxially disposed with the large gear 4241, a plurality of small gears 4211 are disposed on the reagent disk 421 along the circumferential direction of the reagent disk 421, the small gears 4211 can be engaged with the large gear 4241, and the reagent tube is fixedly disposed on the upper portion of the small gear 4211, so that when the reagent disk driver 423 drives the reagent disk 421 to rotate, the small gears 4211 also rotate, and the reagents in the reagent tube can be uniformly mixed.

In another embodiment of the present application, referring to fig. 2, the cup feeding mechanism 210 includes a loading storage portion 211 disposed on the frame 100 for loading and storing reaction cups, a conveying portion 212 disposed on one side of the loading storage portion 211 and interfacing with the loading storage portion 211, and a rotating disk 213 interfacing with the conveying portion 212.

The sample feeding mechanism 410 has a cup feeding position e (see fig. 1), the cup conveying portion 212 of the cup feeding mechanism 210 feeds the reaction cups in the loading and storing portion 211 to the rotating disk 213, the rotating disk 213 transfers the reaction cups to the cup feeding position e, and the conveying mechanism 220 conveys the newly fed reaction cups from the cup feeding position e to the mixing position a of the mixing system 300.

In another embodiment of the present application, referring to fig. 4, the sample feeding mechanism 410 includes a sample feeding tube support 411 and a sample feeding driver 412 (referring to fig. 2), the information collecting position c is located on the sample feeding side of the sample feeding position b (the direction indicated by the arrow in fig. 4 is the sample feeding direction), the sample feeding tube support 411 has a plurality of sample tube slots for accommodating sample tubes, and the sample feeding driver 412 is used for driving the sample feeding tube support 411 to move on the frame 100, so that each sample tube on the sample feeding tube support 411 sequentially passes through the sample feeding position b and each sample tube sequentially passes through the information collecting position c.

In the chemiluminescent immunoassay analyzer provided in this embodiment, during detection, the first information collecting mechanism 450 collects information of a sample tube and sends the collected information of the sample tube to the control system 700, the control system 700 controls the sample feeding mechanism 410 to dispatch the sample tube to the sample sucking position b, the transferring mechanism 430 of the collecting system 400 to transfer the sample in the sample tube on the sample sucking position b to the reaction cup of the mixing position a, then the control system 700 controls the reagent tray mechanism 420 to dispatch the reagent tube matched with the information of the sample tube to the reagent sucking position d, and the transferring mechanism 430 of the collecting system 400 to transfer the reagent in the reagent tube on the reagent sucking position d to the mixing position a of the mixing system 300, so that a proper reagent can be automatically matched for the sample.

Optionally, the sample feeding mechanism 410 has a sample pre-detection placement area 413, a sample feeding track 414, and a sample post-detection placement area 415, where the sample pre-detection placement area 413 and the sample post-detection placement area 415 are opposite and spaced apart, and the sample feeding track 414 connects the two areas and is located on the same side of the two areas. The sample pre-detection placing area 413 and the sample post-detection placing area 415 can both place a plurality of rows of sample tube supports 411 parallel to the sample injection direction, the sample injection driver 412 integrally moves the sample tube supports 411 in the sample pre-detection placing area 413 to the sample injection track 414 along the direction perpendicular to the sample injection direction, the sample tube supports 411 are driven to move by a distance of one sample tube each time along the sample injection direction at the sample injection track 414, and after the sample tube on the sample tube supports 411 completes detection, the sample tube supports 411 are integrally moved to the sample post-detection placing area 415 along the direction perpendicular to the sample injection direction, so that the orderly detection of samples is facilitated.

In another embodiment of the present application, referring to fig. 1 and 4, the sample feeding mechanism 410 further includes an emergency sample tube support 416 and an emergency driver 417, the sample feeding mechanism 410 further has an emergency position f, the emergency position f is located on a movement track of the transfer mechanism 430, an emergency sample tube slot for accommodating an emergency sample tube is provided on the emergency sample tube support 416, and the emergency driver 417 is used for driving the emergency sample tube support to move on the frame 100 so as to make the emergency sample tube enter or leave the emergency position f.

When the chemiluminescent immunoassay analyzer provided by the embodiment is used, the emergency driver 417 drives the emergency sample tube support 416 to a position far away from the emergency position f, an operator places the emergency sample tube in the emergency sample tube slot, the emergency driver 417 drives the emergency sample tube support 416 to a position where the emergency sample tube is located at the emergency position f, and the transfer mechanism 430 transfers the emergency sample in the emergency sample tube located at the emergency position f to the mixing position a, so that the emergency sample can be timely detected, and the function of the chemiluminescent immunoassay analyzer is perfected.

In another embodiment of the present application, referring to fig. 2, the transfer mechanism 430 includes a sample needle 431, a sample arm 432, a sample adding driver 433, a reagent needle 434, a reagent arm 435, and a reagent adding driver 436, wherein the sample needle 431 is disposed at one end of the sample arm 432, the sample adding driver 433 is used for driving the sample arm 432 to move (rotate and lift) so as to move the sample needle 431 to a sample sucking position b or a mixing position a, the reagent needle 434 is disposed at one end of the reagent arm 435, and the reagent adding driver 436 is used for driving the reagent arm 435 to move (rotate and lift) so as to move the reagent needle 434 to a reagent sucking position d or a mixing position a (referring to fig. 1).

The chemiluminescent immunoassay analyzer provided in this embodiment can collect a sample in a sample tube at a sample sucking position b through the sample needle 431, and collect a reagent in a reagent tube at a reagent sucking position d through the reagent needle 434, thereby improving the detection efficiency of the chemiluminescent immunoassay analyzer.

Optionally, when the sample injection mechanism 410 is provided with the emergency position f, the movement track of the sample needle 431 intersects with the emergency position f, and can be used to transfer the emergency sample on the emergency position f to the blending position a.

In another embodiment of the present application, referring to fig. 2, transfer mechanism 430 further includes a first cleaner 437 for cleaning sample pins 431 and a second cleaner 438 for cleaning reagent pins 434.

The chemiluminescent immunoassay analyzer provided in this embodiment can clean the reagent needle 434 after transferring the sample by the first cleaner 437, and clean the reagent needle 434 after transferring the reagent by the second cleaner 438, so that the cleaning operations of the reagent needle 434 and the sample needle 431 are not affected, cross infection is completely avoided, and the detection efficiency is improved.

Referring to fig. 4, because the sample tube is often attached with an information storage carrier such as a barcode for recording information of the sample tube, and the information storage carrier on the sample tube cannot be oriented to the same direction after the sample tube is placed on the sample tube holder 411, the sample tube needs to be rotated by the rotation mechanism 440, so that the first information collecting mechanism 450 can collect information of all the sample tubes on the sample tube holder 411 in the same direction.

In another embodiment of the present application, referring to fig. 4, 7 and 8, the rotating mechanism 440 includes a first shaft 441, a second shaft 442, a rotating wheel 443, two pre-pressing wheels 444, a pre-pressing arm 445 and a position adjusting assembly 446, wherein the first shaft 441 and the second shaft 442 are disposed at two sides of the information collecting position c; a rotation wheel 443 rotatably provided on the first shaft 441 and adapted to abut against an outer surface of the sample tube at the information collection position c to rotate the sample tube; the pre-pressing arm 445 is rotatably sleeved on the second shaft 442, the two pre-pressing wheels 444 are disposed on the pre-pressing arm 445, and the position adjusting component 446 is used for applying an elastic force to the pre-pressing arm 445, so that the pre-pressing arm 445 drives the two pre-pressing wheels 444 to elastically press the outer surface of the sample tube at the information collecting position c.

It should be noted that the pre-pressing wheel 444 may be fixedly provided on the pre-pressing arm 445 or rotatably provided on the pre-pressing arm 445. Compared with the pre-pressing arm 445 fixedly arranged thereon, when the pre-pressing wheel 444 is rotatably arranged on the pre-pressing arm 445, the sliding friction between the pre-pressing wheel 444 and the sample tube can be converted into rolling friction, and the sample tube can more smoothly enter and exit the information collection position c.

It should be further noted that, the first shaft 441 of the rotating wheel 443 may be fixedly disposed on the frame 100, or may be disposed on the frame 100 so as to be close to or far away from the information collecting position c, and when the first shaft 441 of the rotating wheel 443 is movably disposed on the frame 100, compared with the case of being fixedly disposed on the frame 100, the rotating wheel 443 can move in a direction far away from the information collecting position c before the sample tube enters the information collecting position c, so as to make more space for the sample tube, make the sample tube enter the information collecting position c more easily, and move in a direction close to the information collecting position c until abutting against the outer surface of the sample tube after the sample tube enters the information collecting position c, so as to normally drive the sample tube to rotate.

In the chemiluminescent immunoassay analyzer provided in this embodiment, before the sample tube does not enter the information collection position c, the pre-pressing arm 445 and the pre-pressing wheel 444 are at the initial position (the position where the pre-pressing arm 445 is located shown by the solid line in fig. 8) under the action of the position adjusting component 446, during the process that the sample tube enters the information collection position c along the sample direction, the pre-pressing wheel 444 is pushed by the sample tube, the pre-pressing arm 445 is driven by the pre-pressing wheel 444 to rotate around the second shaft 442, the pre-pressing arm 445 and the pre-pressing wheel 444 allow more space for the sample tube until the sample tube completely enters the information collection position c, after the pre-pressing arm 445 and the pre-pressing wheel 444 are kept at the position where the pre-pressing wheel 444 can be abutted against the outer surface of the sample tube under the action of the position adjusting component 446 (the position where the pre-pressing arm 445 is located shown by the broken line in fig. 8), then, the rotating wheel 443 is abutted against the outer surface of the sample tube and drives the sample tube to rotate, after the sample tube completes rotation and leaves the information collection position c, the pre-pressing arm 445 and the pre-pressing wheel 444 returns to the initial position under the action of the position adjusting component 446, so that the rotation operation can be continued on the next sample tube. According to the chemiluminescent immunoassay analyzer provided by the application, the two pre-pressing wheels 444 are arranged on the rotatable pre-pressing arm 445, the position of the pre-pressing arm 445 is regulated by the position regulating component 446, when a sample tube is positioned at the information acquisition position c, the pre-pressing wheels 444 can be abutted against the outer surface of the sample tube and matched with the rotating wheel 443 to complete rotation operation, when the sample tube enters and exits the information acquisition position c, the pre-pressing wheels 444 and the pre-pressing arm 445 can change the positions on the frame 100 under the pushing of the sample tube, so that a moving space is reserved for the sample tube, the sample tube can be enabled to enter and exit the information acquisition position c in a flexible mode, and the clamping is not easy to occur.

In addition, in the chemiluminescent immunoassay analyzer provided in this embodiment, since the positions of the pre-pressing wheel 444 and the pre-pressing arm 445 on the frame 100 can be changed under the action of the sample tube and the position adjusting component 446, the chemiluminescent immunoassay analyzer can be suitable for sample tubes with different diameters, and has a wider application range.

In another embodiment of the present application, referring to fig. 7 and 8, the position adjusting assembly 446 includes a mounting seat 4461, a guide rod 4462 and an elastic element 4463, wherein the mounting seat 4461 is provided with a second shaft 442, and the mounting seat 4461 further has a first limiting surface 4464; the first end of the pre-pressing arm 445 is connected with two pre-pressing wheels 444, and the second end of the pre-pressing arm 445 is provided with a sliding groove 4451; the first end of the guide rod 4462 is in sliding fit with the sliding groove 4451, and the second end of the guide rod 4462 is fixed on the mounting seat 4461; the elastic piece 4463 is sleeved on the guide rod 4462 and is abutted between the mounting seat 4461 and the pre-pressing arm 445, and after the sample tube leaves the information collection position c, the pre-pressing arm 445 is abutted against the first limiting surface 4464 under the action of the elastic piece 4463.

In the chemiluminescent immunoassay analyzer provided in this embodiment, when the sample tube is not in contact with the pre-pressing wheel 444, the pre-pressing arm 445 abuts against the first limiting surface 4464 under the action of the elastic member 4463, when the sample tube enters the information collection position c along the sample feeding direction, the pre-pressing wheel 444 is pushed by the sample tube, the pre-pressing wheel 444 pushes the pre-pressing arm 445 to rotate around the shaft body, the pre-pressing arm 445 applies pressure or pulling force to the elastic member 4463 (when the second end of the guide rod 4462 is arranged on one side of the pre-pressing arm 445 close to the information collection position c, the pre-pressing arm 445 applies pressure to the elastic member 4463, and the second end of the guide rod 4462 is arranged on one side of the pre-pressing arm 445 away from the information collection position c), until the sample tube completely enters the information collection position c, at this time, under the action of the elastic force of the elastic member 4463, the pre-pressing wheel 444 on the pre-pressing arm 445 abuts against the outer surface of the sample tube, the sample tube is driven to rotate by the rotating wheel 443 on the information collection position c, the first information collection mechanism 450 collects information, and then the sample tube leaves the information collection position c along the direction, and the elastic member 445 abuts against the first limiting surface 4464 again under the action of the elastic member 4463. The chemiluminescent immunoassay analyzer provided in this embodiment has a simple structure of the rotating mechanism 440, and a small volume, and greatly saves the cost and design space of the rotating mechanism 440.

Optionally, the position adjusting assembly 446 includes a torsion spring (not shown), which is sleeved on the second shaft 442, one end of the torsion spring is abutted with the frame 100, the other end of the torsion spring is abutted with the pre-pressing arm 445, when the sample tube is not located at the information collecting position c, the pre-pressing arm 445 and the pre-pressing wheel 444 are located at the initial position under the action of the torsion spring, when the sample tube enters the information collecting position c along the sample feeding direction, the sample tube pushes the pre-pressing wheel 444 located at the initial position, the pre-pressing wheel 444 pushes the pre-pressing arm 445 to rotate around the second shaft 442 and presses the torsion spring until the sample tube completely enters the information collecting position c, at this time, the pre-pressing wheel 444 on the pre-pressing arm 445 abuts against the outer surface of the sample tube under the action of the elasticity of the torsion spring, then the sample tube is driven to rotate passively by the rotating wheel 443, and the first information collecting mechanism 450 collects information, then the sample tube leaves the information collecting position c along the sample feeding direction, and the pre-pressing arm 445 and the pre-pressing wheel 444 are reset to the initial position under the action of the elasticity of the torsion spring.

In another embodiment of the present application, referring to fig. 7 and 8, the mounting base 4461 includes a base 4465 and a pressing plate 4466, the pressing plate 4466 is disposed on the base 4465 and can move relative to the base 4465, the pressing plate 4466 or the base 4465 has a first limiting surface 4464, the pressing plate 4466 is provided with a second shaft 442, and the second end of the guide rod 4462 is fixedly connected to the pressing plate 4466.

According to the chemiluminescent immunoassay analyzer provided by the embodiment, the positions of the pressing plate 4466 on the base plate 4465 are changed, so that the positions of the second shaft 442, the pre-pressing arm 445 and the elastic piece 4463 can be synchronously changed, and the position of the pre-pressing wheel 444 for pressing the sample tube is adjusted, so that the position of the pre-pressing wheel 444 is matched with the position of the sample tube, and the adaptability is better; after the position of the pressing plate 4466 on the base plate 4465 is adjusted, the pressure provided by the pre-pressing wheel 444 to the sample tube is kept unchanged, and the pressure provided by the pre-pressing wheel 444 to the sample tube does not need to be readjusted after the position of the pressing plate 4466 is adjusted, so that the rotating mechanism 440 has the advantage of easy adjustment; in addition, the rotating mechanism 440 provided in this embodiment reduces the corresponding motor or cylinder driving mechanism, and has the advantages of simple structure, low cost, small volume and being beneficial to compact design of products.

Optionally, the direction of movement of the platen 4466 is parallel to the sample introduction direction of the sample tube. Thus, adjusting the position of platen 4466 on base plate 4465 in a direction parallel to the sample tube's sample introduction direction can change the position of pre-pressure wheel 444 to compress the sample tube, matching the position of rotation mechanism 440 to the sample tube, compressing the outer surface of the sample tube in the appropriate position.

In another embodiment of the present application, referring to fig. 8, the mounting base 4461 further has a second limiting surface 4467, and the second limiting surface 4467 is used for limiting the movable distance of the first end of the pre-pressing arm 445 in a direction away from the information collecting position c.

The second limiting surface 4467 may be a single surface, or may be two or more surfaces parallel to each other, and may be disposed as required, which is not limited only herein.

According to the chemiluminescent immunoassay analyzer provided by the embodiment, the second limiting surface 4467 cooperates with the first limiting surface 4464 to limit the pre-pressing arm 445 to rotate within a certain range, so that the volume of the rotating mechanism 440 can be reduced as much as possible, and the second limiting surface 4467 can avoid excessive compression or multi-degree stretching of the elastic piece 4463 caused by excessive rotation of the end, provided with the pre-pressing wheel 444, of the pre-pressing arm 445 in a direction away from the information acquisition position c.

In another embodiment of the present application, referring to fig. 7 and 8, the position adjusting assembly 446 further includes an adjusting wheel 4468, wherein the adjusting wheel 4468 is sleeved on the guide rod 4462 and can move along the axial direction of the guide rod 4462, and the elastic member 4463 is abutted between the adjusting wheel 4468 and the pre-pressing arm 445.

It should be noted that, the adjusting wheel 4468 may be disposed on the guide rod 4462 in various manners, for example, in the case that the second end of the guide rod 4462 described in the above embodiment is disposed at the side of the pre-pressing arm 445 near the information collecting position c, and the elastic member 4463 is in a compressed state, the adjusting wheel 4468 is provided with an internal thread, the guide rod 4462 is provided with an external thread adapted to the internal thread, the adjusting wheel 4468 is sleeved on the portion of the guide rod 4462 provided with the external thread, and the adjusting wheel 4468 is rotated to move the adjusting wheel 4468 on the guide rod 4462 in a direction approaching the pre-pressing arm 445, so that the elastic member 4463 is further contracted, and the pressure of the pre-pressing wheel 444 on the pre-pressing arm 445 on the outer surface of the sample tube is increased; rotating the adjustment wheel 4468 causes the adjustment wheel 4468 to move on the guide rod 4462 in a direction away from the preload arm 445, which causes the elastic member 4463 to extend, thereby reducing the pressure of the preload wheel 444 on the preload arm 445 against the outer surface of the sample tube. For another example, the adjusting wheel 4468 is slidably sleeved on the guide rod 4462, a row of positioning holes (not shown) are axially arranged on the guide rod 4462, after the adjusting wheel 4468 is moved to a required position, positioning pieces (not shown) can be inserted into the corresponding positioning holes, and the adjusting wheel 4468 is fixed at the required position by the positioning pieces.

According to the chemiluminescent immunoassay analyzer provided by the embodiment, the pressing force of the pre-pressing wheel 444 on the sample tube can be adjusted by changing the position of the adjusting wheel 4468 on the guide rod 4462, so that the difficulty in adjusting the pre-tightening force is greatly reduced, and the chemiluminescent immunoassay analyzer is simple in structure and convenient to operate.

In another embodiment of the present application, referring to fig. 7 and 8, when the sample tube leaves the information collection station c (the pre-pressing arm 445 is located at the position where the pre-pressing arm 445 is located as shown by the solid line in fig. 8), the vertical distance between the pre-pressing wheel 444 located at the sample introduction side of the information collection station c and the straight line located in the sample introduction direction is greater than the vertical distance between the pre-pressing wheel 444 located at the discharge side of the information collection station c and the straight line located in the sample introduction direction.

According to the chemiluminescent immunoassay analyzer provided by the embodiment, the connecting line of the centers of the two pre-pressing wheels 444 and the sample injection direction are inclined when the sample tube is not positioned at the information acquisition position c, so that the sample tube can enter the information acquisition position c more smoothly when the sample tube gradually moves to the direction close to the information acquisition position c along the sample injection direction.

Optionally, in a state that the sample tube completely leaves the information collection position c, an included angle between a connecting line of centers of the two pre-pressing wheels 444 and a sample injection direction of the sample tube is 2 ° -15 °.

In another embodiment of the present application, referring to fig. 7 and 8, a groove 4452 is provided on the pre-compression arm 445 between the pre-compression wheels 444.

It should be noted that the size of the groove 4452 may be set according to the external dimension of the sample tube, which is not limited herein.

According to the chemiluminescent immunoassay analyzer provided by the embodiment, the groove 4452 is arranged, so that the sample tube can enter the information collection position c more smoothly when gradually moving towards the direction close to the information collection position c along the sample injection direction.

In another embodiment of the present application, referring to fig. 1 and 2, an incubation and cleaning system 500 includes a pot body 510, a cleaning tray 520, an incubation tray 530, an incubation driver 540, a cleaning driver 550 and a cleaning module 560, wherein the pot body 510 is disposed on the rack 100, the incubation tray 530 and the cleaning tray 520 are coaxially disposed in the pot body 510, the incubation tray 530 is disposed above the cleaning tray 520, the inner diameter of the incubation tray 530 is smaller than that of the cleaning tray 520, an incubation groove for accommodating a reaction cup is disposed at the upper portion of the incubation tray 530, a cleaning groove for accommodating the reaction cup is disposed at the portion of the cleaning tray 520 beyond the incubation tray 530, the incubation driver 540 is used for driving the incubation tray 530 to rotate, and the cleaning driver 550 is used for driving the reaction cup on the cleaning tray 520 to the cleaning module 560 to perform cleaning operation, substrate filling operation and mixing operation.

Specifically, the incubation cleaning system 500 further includes a rotation shaft 570 and a rotation sleeve 580, the rotation sleeve 580 is rotatably sleeved on the rotation shaft 570 through a bearing, the incubation plate 530 is fixedly sleeved on the periphery of the rotation shaft 570, and the incubation driver 540 is disposed below the pot 510 and is used for sequentially driving the rotation shaft 570 and the incubation plate 530; the washing tray 520 is fixedly sleeved on the outer circumference of the rotating sleeve 580, and the washing driver 550 is disposed below the pot body 510 and is used for sequentially driving the rotating sleeve 580 and the washing tray 520.

The chemiluminescent immunoassay analyzer provided in this embodiment, through reasonable structural design, makes incubation operation and cleaning operation performed in the same pot body 510 and not affected each other, and is beneficial to compact design of products.

Optionally, the pot 510 of the incubation and washing system 500 includes a pot bottom 511 and a cover 512, and an incubation position g for placing or taking out the reaction cup into or from the incubation slot and a washing position h for placing the reaction cup into or from the washing slot are provided on the cover 512.

In another embodiment of the present application, referring to fig. 5 and 6, the cleaning module 560 includes a first lifting plate 561, a plurality of liquid sucking needles 562 disposed on the first lifting plate 561 along a circumferential direction of the first lifting plate 561, a second lifting plate 563, and liquid injecting needles 564 disposed on the second lifting plate 563 along a circumferential direction of the second lifting plate 563 and having the same number as the liquid sucking needles 562, wherein the liquid sucking needles 562 and the liquid injecting needles 564 are alternately disposed, a liquid sucking position i is below the liquid sucking needles 562, a liquid injecting position j is below the liquid injecting needles 564, and the cleaning disc 520 drives the reaction cups to suck the liquid at the liquid sucking positions i and drives the reaction cups to inject the liquid at the liquid injecting positions j when rotating.

It should be noted that, the cleaning module 560 further includes a magnetic separation member (not shown) disposed on a side wall of the pot body 510, the reaction cup is filled with the cleaning solution by the filling needle 564 at the filling level j, the reaction cup rotates to the liquid sucking level i, and the magnetic beads in the reaction cup located at the liquid sucking level i are adsorbed on the wall of the reaction cup under the magnetic field of the magnetic separation member, so that the loss of the magnetic beads can be reduced during the liquid sucking operation, and the accuracy of the subsequent detection is ensured.

In the chemiluminescent immunoassay analyzer provided in this embodiment, the liquid suction needle 562 is driven by the first lifting plate 561, and the liquid injection needle 564 is driven by the second lifting plate 563, so that the liquid injection operation and the liquid suction operation are not affected by each other.

Alternatively, the first elevating plate 561 and the second elevating plate 563 are driven to move up and down by a linear motor.

Optionally, be provided with four imbibition needles 562 on the first lifter plate 561, be provided with four notes liquid needles 564 on the second lifter plate 563, can carry out the quadruple washing to the reaction liquid in the reaction cup, wash abundant effectual, be favorable to promoting the detection precision.

In another embodiment of the present application, referring to FIG. 5, the incubation and washing system 500 further comprises two substrate liquid filling tubes 590, wherein the two substrate liquid filling tubes 590 are used to fill the substrate liquid required for direct chemiluminescence and the substrate liquid required for enzymatic chemiluminescence, respectively, into the reaction cup after the washing operation.

The substrate solution required for direct chemiluminescence may be hydrogen peroxide or sodium hydroxide solution, and the substrate solution required for enzymatic chemiluminescence may be luminol, etc., and may be selected according to need, and is not limited only herein.

The chemiluminescent immunoassay analyzer provided by the embodiment can be suitable for direct chemiluminescent analysis and enzymatic chemiluminescent analysis, and has good adaptability.

In another embodiment of the present application, referring to fig. 1, 9 and 10, the light measurement system 600 includes a camera lens 610, a light shielding driver 620, a light shielding component 630 and a photomultiplier 640, wherein the camera lens 610 has a receiving cavity 611, a first light hole 612 and a second light hole 613 are disposed on a wall of the receiving cavity 611, the light shielding driver 620 is disposed on the camera lens 610, and one end of an output shaft of the light shielding driver 620 is disposed in the receiving cavity 611; the photomultiplier 640 is installed in the first light hole 612 and receives the light signal through the first light hole 612, the shading component 630 is arranged in the accommodating cavity 611, the shading component 630 comprises a shading arm 631 and a swinging arm 632, a locating pin 614 is arranged on the cavity wall of the accommodating cavity 611, the shading arm 631 is rotatably sleeved on the locating pin 614, one end of the swinging arm 632 is connected to the output shaft of the driver, and the other end of the swinging arm 632 is used for driving the shading arm 631 to rotate, so that the shading arm 631 shields the second light hole 613 or moves away from the second light hole 613.

Optionally, the light measurement system 600 is integrally installed at a side portion of the pan body 510, and a mounting hole 513 (see fig. 6) is provided on a side wall of the pan body 510, and the second light-transmitting hole 613 of the camera bellows 610 can receive light emitted from substances in the reaction cup, which have completed incubation operation, cleaning operation, substrate filling operation and mixing operation in the pan body 510, through the mounting hole 513.

When the chemiluminescent immunoassay analyzer provided by the application needs to be tested by adopting the photomultiplier 640, the light shielding driver 620 drives the swing arm 632, and the swing arm 632 drives the light shielding arm 631 to move away from the second light transmission hole 613, so that a light signal to be tested enters a detection port of the photomultiplier 640 through the second light transmission hole 613 and the first light transmission hole 612 in sequence, the photomultiplier 640 can complete the test under the state without environment light, and the test precision is high; when the photomultiplier 640 needs to be installed or maintained, the light shielding driver 620 drives the swing arm 632, the swing arm 632 drives the light shielding arm 631 to move to shield the second light transmission hole 613, so that no light enters the accommodating cavity 611, and at the moment, the photomultiplier 640, the camera bellows 610, the light shielding driver 620 and the light shielding component 630 are installed and maintained together from the pan body 510, and the photocathode of the detection port of the photomultiplier 640 is always in a dark environment and cannot be damaged.

In another embodiment of the present application, referring to fig. 1 and 2, the chemiluminescent immunoassay analyzer further comprises a cup system 800, the cup system 800 comprising a waste recovery mechanism 810 for recovering substances within the used reaction cup and a waste cup recovery mechanism 820 for recovering the used reaction cup.

The cover 512 of the pot 510 of the incubation and cleaning system 500 is provided with a cup taking position k (see fig. 5) for taking out the reaction cup with the light measurement from the pot 510, a cup taking opening of the reaction cup is taken out by a cup recycling mechanism 820, the waste liquid in the reaction cup is recycled by a waste liquid recycling mechanism 810, and the reaction cup is discarded to a reaction cup collecting box by the cup recycling mechanism 820 through a cup throwing position m (see fig. 1).

In another embodiment of the present application, referring to fig. 1, the handling mechanism 220 may move the reaction cup in a horizontal direction and a vertical direction, and the movement track of the handling mechanism 220 intersects at least the mixing position a, the cup feeding position e, the incubation position g, the cleaning position h and the cup taking position k of the waste cup, and the reaction cup is handled between the above positions through the cup clamping and pushing functions.

One of the test procedures of the chemiluminescent immunoassay analyzer provided in this embodiment is as follows:

Referring to fig. 11 in combination, the analyzer is started, the analyzer is initialized, the whole analysis process starts, the transfer part 212 of the cuvette feeding mechanism 210 feeds the cuvette in the loading and storing part 211 to the rotating disc 213, the rotating disc 213 rotates to bring the cuvette to the cuvette feeding position e, the carrying mechanism 220 carries the newly fed cuvette from the cuvette feeding position e to the mixing position a of the mixing system 300, the sample feeding tube holder 411 located in the sample pre-detection placement area 413 is driven to the sample feeding track 414 by the sample feeding driver 412 of the sample feeding mechanism 410, the sample is moved to the sample sucking position b on the sample feeding track 414, the sample on the sample sucking position b is transferred to the mixing position a by the sample feeding driver 433 of the transfer mechanism 430, the matched reagent tube is driven to the reagent sucking position d by the reagent feeding driver 423 of the reagent disc mechanism 420, the reagent tube in the reagent sucking position d is transferred to the mixing position a by the reagent feeding driver 436 of the transfer mechanism 430, mixing system 300 mixes the liquid in the reaction cup on mixing position a, after mixing, carrying mechanism 220 carries the reaction cup on mixing position a to incubation cleaning system 500, and puts the reaction cup into incubation groove from incubation position g of incubation cleaning system 500 for incubation operation, carrying mechanism 220 takes the reaction cup out of pan 510 from incubation position g after incubation operation is completed, puts into washing tank through washing position h, washes the reaction liquid in the reaction cup by washing module 560, after washing operation is completed, fills substrate liquid needed by substrate liquid filling tube 590 into the reaction cup, and mixes by incubation cleaning system 500 self-contained mixing structure, light measuring system 600 directly carries out optical detection to the substance in the reaction cup after mixing is completed, the used cuvettes and the contents of the cuvettes are recovered by the system 800. The whole detection process is automatically completed, and the influence of human factors on the accuracy and reliability of detection can be avoided.

The foregoing description of the preferred embodiments of the application is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the application.

Claims

1. The chemiluminescent immunoassay analyzer is characterized by comprising a rack, a reaction cup feeding and conveying system, a mixing system, a collecting system, an incubation and cleaning system, a photometry system and a control system, wherein the reaction cup feeding and conveying system, the mixing system, the collecting system, the incubation and cleaning system, the photometry system and the control system are arranged on the rack;

The control system is respectively and electrically connected with the reaction cup feeding and conveying system, the mixing system, the acquisition system, the incubation and cleaning system and the photometry system;

The transfer mechanism comprises a sample needle, a sample arm, a sample adding driver, a reagent needle, a reagent arm and a reagent adding driver, wherein the sample needle is arranged at one end of the sample arm, the sample adding driver is used for driving the sample arm to move so that the sample needle moves to the sample sucking position or the mixing position, the reagent needle is arranged at one end of the reagent arm, and the reagent adding driver is used for driving the reagent arm to move so that the reagent needle moves to the reagent sucking position or the mixing position;

The rotating mechanism comprises a first shaft, a second shaft, a rotating wheel, a pre-pressing arm, two pre-pressing wheels and a position adjusting assembly, wherein the first shaft and the second shaft are arranged on two sides of the information acquisition position; the rotating wheel is rotatably arranged on the first shaft and used for abutting against the outer surface of the sample tube positioned at the information acquisition position so as to rotate the sample tube; the pre-pressing arm is rotatably sleeved on the second shaft, the two pre-pressing wheels are arranged on the pre-pressing arm, the position adjusting assembly is used for applying an elastic force to the pre-pressing arm so that the pre-pressing arm drives the two pre-pressing wheels to elastically press the outer surface of the sample tube positioned at the information acquisition position;

The position adjusting assembly comprises a mounting seat, a guide rod and an elastic piece, wherein the mounting seat is provided with the second shaft and is also provided with a first limiting surface; the first end of the pre-pressing arm is connected with two pre-pressing wheels, and the second end of the pre-pressing arm is provided with a sliding groove; the first end of the guide rod is in sliding fit with the chute, and the second end of the guide rod is fixed on the mounting seat; the elastic piece is sleeved on the guide rod and is abutted between the mounting seat and the pre-pressing arm, and after the sample tube leaves the information acquisition position, the pre-pressing arm is abutted to the first limiting surface under the action of the elastic piece;

The light measuring mechanism comprises a camera bellows, a shading driver, a shading component and a photomultiplier, wherein the camera bellows is provided with a containing cavity, a first light hole and a second light hole which are oppositely arranged are formed in the cavity wall of the containing cavity, the shading driver is arranged on the camera bellows, and one end of an output shaft of the shading driver is arranged in the containing cavity; the photomultiplier install in first light trap department and pass through first light trap receives the light signal, shading subassembly set up in the inside of holding chamber, shading subassembly includes shading arm and swing arm, be equipped with the locating pin on the chamber wall of holding chamber, shading arm rotationally overlaps to locate on the locating pin, the one end of swing arm connect in shading driver's output shaft, just the other end of swing arm is used for driving shading arm is rotatory, so that shading arm shelters from the second light trap or follow second light trap department removes.

2. The chemiluminescent immunoassay analyzer of claim 1, wherein the transfer mechanism further comprises a first cleaner for cleaning the sample needle and a second cleaner for cleaning the reagent needle.

3. The chemiluminescent immunoassay analyzer of claim 1 wherein the incubation and cleaning system comprises a pan body, a cleaning plate, an incubation driver, a cleaning driver and a cleaning module, wherein the pan body is arranged on the frame, the incubation plate and the cleaning plate are coaxially arranged in the pan body, the incubation plate is positioned above the cleaning plate and the inner diameter of the incubation plate is smaller than that of the cleaning plate, an incubation groove for accommodating the reaction cup is arranged on the upper portion of the incubation plate, a cleaning groove for accommodating the reaction cup is arranged at the position, exceeding the incubation plate, of the cleaning plate, the incubation driver is used for driving the incubation plate to rotate, and the cleaning driver is used for driving the cleaning plate to rotate so as to drive the reaction cup on the cleaning plate to the cleaning module for cleaning operation, substrate filling operation and uniform mixing operation.

4. The chemiluminescent immunoassay analyzer of claim 3 wherein the cleaning module comprises a first lifting plate, a plurality of liquid suction needles arranged on the first lifting plate along the circumferential direction of the first lifting plate, a second lifting plate and liquid injection needles which are arranged on the second lifting plate along the circumferential direction of the second lifting plate and have the same number as the liquid suction needles, wherein the liquid suction needles and the liquid injection needles are alternately arranged, the liquid suction level is arranged below the liquid suction needles, the liquid injection level is arranged below the liquid injection needles, and the cleaning disc drives the reaction cup to suck liquid at each liquid suction position and drives the reaction cup to inject liquid at each liquid injection level when rotating.

5. The chemiluminescent immunoassay analyzer of claim 1 wherein the incubation and wash system further comprises two substrate liquid filling tubes for filling the reaction cup with a substrate liquid required for direct chemiluminescence and a substrate liquid required for enzymatic reaction chemiluminescence, respectively, after a wash operation.

6. The chemiluminescent immunoassay analyzer of any one of claims 1-5, further comprising at least one of:

The sample injection mechanism comprises an emergency sample tube support and an emergency driver, the sample injection mechanism is also provided with an emergency position, the emergency position is positioned on the motion track of the transfer mechanism, the emergency sample tube support is provided with an emergency sample tube slot for accommodating an emergency sample tube, and the emergency driver is used for driving the emergency sample tube support to move on the frame so as to enable the emergency sample tube on the emergency sample tube support to enter or leave the emergency position;

The sample feeding mechanism further comprises a sample feeding sample tube support and a sample feeding driver, the information acquisition position is located at the sample feeding side of the sample feeding position, the sample feeding sample tube support is provided with a plurality of sample tube grooves for accommodating the sample tubes, and the sample feeding driver is used for driving the sample tube support to move on the frame so that each sample tube on the sample feeding sample tube support sequentially passes through the sample feeding position and each sample tube sequentially passes through the information acquisition position;

The cup feeding mechanism comprises a loading storage part, a conveying part and a rotating disc, wherein the loading storage part is arranged on the rack and used for loading and storing the reaction cup, the conveying part is arranged on one side of the loading storage part and is in butt joint with the loading storage part, and the rotating disc is in butt joint with the conveying part; and

The chemiluminescent immunoassay analyzer further comprises a cup throwing system, wherein the cup throwing system comprises a waste liquid recovery mechanism for recovering substances in the used reaction cup and a waste cup recovery mechanism for recovering the used reaction cup.