CN104777321B - Chemiluminescence immune assay device - Google Patents

Chemiluminescence immune assay device Download PDF

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Publication number
CN104777321B
CN104777321B CN201510181011.9A CN201510181011A CN104777321B CN 104777321 B CN104777321 B CN 104777321B CN 201510181011 A CN201510181011 A CN 201510181011A CN 104777321 B CN104777321 B CN 104777321B
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China
Prior art keywords
sample
reaction
reagent
disc
needle
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CN201510181011.9A
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Chinese (zh)
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CN104777321A (en
Inventor
杨东超
刘平
叶翀
王峤
袁振清
吴小胜
罗晓琴
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Tsinghua University
Beijing Kwinbon Biotechnology Co Ltd
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Tsinghua University
Beijing Kwinbon Biotechnology Co Ltd
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Priority to CN201510181011.9A priority Critical patent/CN104777321B/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention provides a kind of chemiluminescence immune assay device, it includes hatching mechanism, sample charging mechanism and luminous detection mechanism for model machine structure, chemical reaction.Include for model machine structure: multiple sample cells;Specimen disc, in annular and can rotate;Multiple Reagent Tubes;And reagent disc, in annular and can rotate.Chemical reaction is hatched mechanism and is set to constant temperature, including: rotating disk, rounded;Multiple reaction tray, the circumference along rotating disk is equally spaced on rotating disk, and each reaction tray is circumferentially, equally spaced provided with reaction cup and holds chamber;Multiple reaction cup, the circumference along each reaction tray is equally spaced;And driving mechanism, power connects rotating disk and the plurality of reaction tray, so that turntable rotation is thus driving all reaction tray to revolve round the sun together or making each rotation of all reaction tray.Sample charging mechanism includes rectilinear movement unit, sample pin, reagent pin and cleaning groove.Luminous detection mechanism includes cleaning needle and substrate pin, light shade cover and detection head.

Description

Chemiluminescence immunoassay device
Technical Field
The invention relates to a chemiluminescence immune detection system, in particular to a chemiluminescence immune analysis device.
Background
The chemiluminescence immunoassay (CLIA) is a recent immunoassay developed following radioimmunoassay, enzyme immunoassay, fluorescence immunoassay, and the like. Chemiluminescence immunoassay consists of immunoassay and chemiluminescence assay. The immunoassay system directly marks chemiluminescent substances or enzymes on antigens or antibodies as markers, and forms antigen-antibody immune complexes through the reaction of the antigens and the antibodies. The chemiluminescence system is characterized in that after the immunoreaction is finished, a luminous substrate of an oxidant or an enzyme is added, a chemiluminescent substance is oxidized by the oxidant to form an intermediate in an excited state, photons are emitted to release energy to return to a stable ground state, the luminous intensity can be detected by using a luminous signal measuring instrument, and the content of a detected object can be calculated by using a standard curve according to the relation between a chemiluminescent marker and the luminous intensity. CLIA has the characteristics of high sensitivity, strong specificity, wide linear range, simple and convenient operation, no need of expensive instruments and equipment and the like, has wide application range, and is widely applied to a plurality of fields such as environment, clinic, food, drug detection and the like.
The research in the aspect of European and American countries starts earlier, the existing product models are more, the technology is mature, and most markets are occupied. The CLIA technology is introduced in the last 90 th century in China, and relevant research works are only developed in individual hospitals and research institutes at that time, instruments used mainly depend on import, and relatively mature products are not available in China; however, after the 21 st century, with the widespread use of CLIA technology, the demand for chemiluminescent immunoassay analyzers has increased. Therefore, there is a great trend toward the development of chemiluminescent immunoassay analyzers.
However, the current chemiluminescence immunoassay analyzer in China partially adopts a guide rail type feeding mechanism, which results in larger size of the analyzer. The reagent in the reaction cup is uniformly mixed by the transmission of the gear and the rack, and the noise is high. And low efficiency and low flux.
Disclosure of Invention
In view of the problems of the background art, it is an object of the present invention to provide a chemiluminescent immunoassay device which can greatly reduce the space occupied by the whole chemiluminescent immunoassay device, and it is another object of the present invention to provide a chemiluminescent immunoassay device which can greatly increase the analysis flux and greatly improve the working efficiency.
It is another object of the present invention to provide a chemiluminescent immunoassay device which can be positioned with high precision.
In order to achieve the above object, the present invention provides a chemiluminescent immunoassay device, which comprises a sample feeding mechanism, a chemical reaction incubation mechanism, a sample feeding mechanism and a luminescence detection mechanism.
The sample supply mechanism comprises: the sample tubes are used for containing samples to be detected; the sample disc is annular and can rotate, and an accommodating cavity for accommodating a sample tube is arranged along the circumferential direction of the sample disc; a plurality of reagent tubes, each reagent tube for holding a reagent; and the reagent disk is annular and can rotate, is concentrically arranged on the inner side of the sample disk, and is provided with a containing part for containing the reagent tube along the circumferential direction.
The chemical reaction incubation mechanism is set to be at a constant temperature, and comprises: the rotating disc is circular and is opposite to the sample disc and the reagent disc; the reaction discs are arranged on the rotating disc at equal intervals along the circumferential direction of the rotating disc, and each reaction disc is provided with a reaction cup accommodating cavity at equal intervals along the circumferential direction; a plurality of reaction cups which are arranged along the circumferential direction of each reaction disk at equal intervals, and each reaction cup is inserted into and fixedly held in a reaction cup accommodating cavity on the corresponding reaction disk; and the driving mechanism is in power connection with the rotating disc and the reaction discs so as to enable the rotating disc to rotate to drive all the reaction discs to revolve together or enable all the reaction discs to rotate independently.
The application of sample mechanism includes: the linear moving unit is arranged above the sample disc, the reagent disc and the rotary disc of the sample supplying mechanism along the direction from the common circle center of the sample disc and the reagent disc of the sample supplying mechanism to the circle center of the rotary disc; the sample needle is in power connection with the linear moving unit, can be driven by the linear moving unit to linearly reciprocate and can move up and down along the direction from the common circle center of the sample plate and the reagent plate of the sample supplying mechanism to the circle center of the rotating disc so as to suck a sample from a sample tube contained on the sample plate and add the sample into a reaction cup on a reaction plate at a sample adding position below the linear moving unit; the reagent needle is fixedly and dynamically connected with the sample needle at intervals, can linearly reciprocate and can move up and down along the direction from the common circle center of the sample disc and the reagent disc of the sample supplying mechanism to the circle center of the rotary disc under the driving of the linear moving unit, so as to suck a reagent from a reagent tube contained on the reagent disc and add the reagent into a reaction cup on a reaction disc at a sample adding position below the linear moving unit; and a cleaning tank which is positioned below the linear moving unit and between the sample supply mechanism and the chemical reaction incubation mechanism and is used for cleaning the sample needle for completing the sample addition and the reagent needle for completing the reagent addition.
The light emission detection mechanism includes: the cleaning needle and the substrate needle are arranged above one corresponding reaction disc, are sequentially arranged along the circumferential direction of the reaction disc in a manner of aligning with one part of a plurality of reaction cups arranged on the circumferential direction of the reaction disc, and can respectively move along the up-down direction; the light shielding cover can pivot in a horizontal plane and can ascend and descend in the vertical direction, is correspondingly arranged above the cleaning needle and the substrate needle, and is used for shielding the cleaning needle, the substrate needle, the corresponding reaction disc after incubation and all reaction cups on the reaction disc; and the detection head is arranged in the light shielding cover and is used for carrying out luminescence detection on reactants in the reaction cup added with the substrate.
Wherein,
the sample tray and the reagent tray of the sample supply mechanism rotate relatively, so that corresponding sample tubes on the sample tray and corresponding reagent tubes on the reagent tray are aligned in the radial direction and are positioned below the linear moving unit, and the linear moving unit drives the sample needles and the reagent needles to move linearly to the positions above the corresponding sample tubes and reagent tubes along the direction from the common circle center of the sample tray and the reagent tray of the sample supply mechanism to the circle center of the rotary table and align to the corresponding sample tubes and reagent tubes respectively; then, the linear moving unit drives the sample needle and the reagent needle to move downwards simultaneously so as to suck the sample in the sample tube and the reagent in the reagent tube respectively; then the linear moving unit drives the sample needle and the reagent needle to move upwards, and drives the sample needle and the reagent needle to move towards the rotary table together along the direction from the common circle center of the sample plate and the reagent plate of the sample feeding mechanism to the circle center of the rotary table;
the driving mechanism of the chemical reaction incubation mechanism drives the rotating disc to rotate, so that the reaction discs revolve and one of the reaction discs reaches the lower part of the linear moving unit, and the corresponding reaction cup on the reaction disc is positioned below the linear moving unit and reaches the sample adding position;
the linear moving unit drives the sample needle for absorbing the sample and the reagent needle for absorbing the reagent to move towards the upper part of the reaction cup at the sample adding position along the direction from the common circle center of the sample disc and the reagent disc of the sample supplying mechanism to the circle center of the rotating disc, when the sample needs to be added, the sample needle reaches the upper part of the reaction cup at the sample adding position, then the linear moving unit drives the sample needle for absorbing the sample and the reagent needle for absorbing the reagent to simultaneously move downwards so as to add the sample in the sample needle into the reaction cup at the sample adding position, when the reagent needs to be added, the reagent needle reaches the upper part of the reaction cup at the sample adding position, and then the linear moving unit drives the sample needle and the reagent needle for absorbing the reagent to simultaneously move downwards so as to add the reagent in the reagent needle into the reaction cup at the sample adding position;
after the reaction cups of one reaction disc positioned at the sample adding position finish adding the sample and the reagent, the reaction disc rotates for an angle, so that the other reaction cup of the reaction disc rotates to the sample adding position;
the sample needle for completing the sample addition and the reagent needle for completing the reagent addition move along the direction opposite to the direction from the common circle center of the sample disc and the reagent disc of the sample supplying mechanism to the circle center of the rotating disc under the driving of the linear moving unit and move to the upper part of the cleaning groove; then, the linear moving unit drives the sample needle completing the sample addition and the reagent needle completing the reagent addition to move down into the cleaning tank for cleaning; after cleaning is finished, the linear moving unit drives the cleaned sample needle and the cleaned reagent needle to move upwards to return, and then the linear moving unit drives the returned sample needle and the returned reagent needle to move together towards the sample tray and the reagent tray of the sample supply mechanism so as to perform next sample suction and reagent suction and add the reagent and the sample to the other reaction cup of the reaction tray at the sample adding position;
after all reaction cups in the reaction disc at the sample adding position complete the adding of the reagent and the adding of the sample, the driving mechanism drives the rotating disc to rotate for an angle so as to make all the reaction discs revolve for an angle, thereby enabling the reaction discs to shift, enabling all the reaction cups on the reaction discs to be in an incubation state, enabling the other reaction disc to be at the sample adding position, and enabling one reaction cup of the other reaction disc to rotate to the sample adding position so as to add the reagent and the sample;
when the turntable enables all the reaction disks to revolve, the light shielding cover pivots upwards to avoid the reaction disks during revolution, after all the reaction disks finish revolution, each reaction disk reaches a specified position, one reaction disk reaches the sample adding position, the reaction disk corresponding to the reaction cup which finishes incubation reaches the position below the position where the light shielding cover is located, the light shielding cover pivots downwards to shield the reaction disks, then the reaction cups are sequentially located below the cleaning needle and the substrate needle along with the rotation of the reaction disks, the cleaning needle moves downwards along with the rotation of the reaction disks to add cleaning liquid into the reaction cups, and the cleaning needle sucks out redundant liquid and moves downwards along with the rotation of the reaction disks to add substrates into the reaction cups; finally, the detection head performs luminescence detection on the reactant in the reaction cup after the substrate is added.
The invention has the following beneficial effects:
compare prior art's guide tracked feeding mechanism, the sample dish and the reagent dish of supply appearance mechanism all adopt ring shape and adopt the carousel that chemical reaction hatched the mechanism, a plurality of reaction dish on the carousel can rotation and revolution, greatly reduce the shared space of whole chemiluminescence immunoassay device, the detection flux has been improved greatly, owing to hatch on the carousel, the setting of the processing station that singly goes on hatching among the prior art has been cancelled, thereby greatly improved work efficiency.
By adopting the linear moving unit, the annular sample disc, the annular reagent disc and the reaction disc capable of rotating and revolving, high-precision positioning can be realized.
Drawings
FIG. 1 is a top view of a chemiluminescent immunoassay device according to the present invention;
FIG. 2 is a perspective view of a sample supply mechanism and a sample information identification mechanism of the chemiluminescent immunoassay device according to the present invention;
FIG. 3 is a perspective view of a handling mechanism of the chemiluminescent immunoassay device according to the present invention;
FIG. 4 is a perspective view of the cleaning needle and the substrate needle of the chemical reaction incubation mechanism and the luminescence detection mechanism of the chemiluminescent immunoassay device according to the present invention with one reaction cup removed to expose the reaction cup receiving chamber for clarity;
FIG. 5 is another perspective view of the chemical reaction incubation mechanism of the chemiluminescent immunoassay device according to the present invention.
Wherein the reference numerals are as follows:
1 sample supply mechanism 31 straight line moving unit
11 sample tube 32 sample needle
12 sample tray 33 reagent needle
121 accommodating cavity 34 cleaning groove
13 luminous detection mechanism of reagent pipe 4
14 reagent disk 41 cleaning needle
141 holding section 411 cleaning needle
15 reaction disc recovery area 412 washs suction pin
2 chemical reaction incubation mechanism 42 substrate needle
21 turntable 43 light-shielding cover
211 through hole 44 detection head
22 reaction disc 5 storage mechanism
221 reaction cup accommodating cavity 51 reaction disc storage bin
222 clamping hole 23 reaction cup 52 sample tube storage bin
24 drive mechanism 521 receptacle
241 motor 6 conveying mechanism
242 master gear 60 guide
243 pinion 61 slide
244 big arm of belt pulley 62
245 small arm of belt 63
246 electromagnetic clutch 64 tweezers
25 connecting rod 65 electromagnet
26 round base 7 sample information identification mechanism
3 sample adding mechanism 71 camera
72 support frame
Detailed Description
The chemiluminescent immunoassay device according to the present invention is described in detail below with reference to the accompanying drawings.
Referring to fig. 1 to 5, the chemiluminescent immunoassay device according to the present invention comprises a sample feeding mechanism 1, a chemical reaction incubation mechanism 2, a sample feeding mechanism 3 and a luminescence detection mechanism 4. The sample supply mechanism 1 includes: a plurality of sample tubes 11, each sample tube 11 being for containing a sample to be tested; the sample disc 12 is annular and can rotate, and an accommodating cavity 121 for accommodating the sample tube 11 is arranged along the circumferential direction of the sample disc 12; a plurality of reagent tubes 13, each reagent tube 13 for holding a reagent; the reagent disk 14 is provided concentrically inside the sample disk 12, and has a ring shape that can rotate, and a storage portion 141 that stores the reagent tube 13 is provided along the circumferential direction. The chemical reaction incubation mechanism 2 is set at a constant temperature and includes: a rotary plate 21 having a circular shape and opposing the sample plate 12 and the reagent plate 14; a plurality of reaction disks 22 provided on the turntable 21 at equal intervals in the circumferential direction of the turntable 21, each reaction disk 22 being provided with a reaction cup accommodating chamber 221 at equal intervals in the circumferential direction; a plurality of reaction cups 23 arranged at equal intervals along the circumferential direction of each reaction tray 22, each reaction cup 23 being inserted into and held in one reaction cup accommodating chamber 221 on a corresponding reaction tray 22; and a driving mechanism 24 for power-connecting the rotary plate 21 and the plurality of reaction discs 22 so as to rotate the rotary plate 21 to drive all the reaction discs 22 to revolve together or to make all the reaction discs 22 rotate independently. The sample adding mechanism 3 includes: the linear moving unit 31 is arranged above the sample disc 12, the reagent disc 14 and the rotary disc 21 of the sample feeding mechanism 1 along the direction from the common circle center of the sample disc 12 and the reagent disc 14 of the sample feeding mechanism 1 to the circle center of the rotary disc 21; a sample needle 32, which is dynamically connected to the linear moving unit 31, and can be driven by the linear moving unit 31 to linearly reciprocate and move up and down along the direction from the common center of the sample tray 12 and the reagent tray 14 of the sample feeding mechanism 1 to the center of the rotary disk 21, so as to suck a sample from the sample tube 11 accommodated on the sample tray 12 and add the sample into one reaction cup 23 on one reaction tray 22 at the sample adding position under the linear moving unit 31; a reagent needle 33, which is connected to the linear moving unit 31 with a fixed distance from the sample needle 32, can be driven by the linear moving unit 31 to reciprocate linearly in a direction from a common center of the sample tray 12 and the reagent tray 14 of the sample feeding mechanism 1 to a center of the rotary disk 21, and can move up and down, so as to suck a reagent from the reagent tube 13 accommodated on the reagent tray 14 and add the reagent into one cuvette 23 on one reaction disk 22 at a sample adding position under the linear moving unit 31; and a cleaning bath 34 located below the linear moving unit 31 and between the sample supply mechanism 1 and the chemical reaction incubation mechanism 2, for cleaning the sample needle 32 where the sample addition is completed and the reagent needle 33 where the reagent addition is completed. The light emission detection mechanism 4 includes: a cleaning needle 41 and a substrate needle 42, which are disposed above a corresponding one of the reaction disks 22, are sequentially disposed along the circumferential direction of the reaction disk 22 in such a manner as to be aligned with a part of the plurality of reaction cups 23 disposed in the circumferential direction on the reaction disk 22, and are respectively movable in the up-down direction, the cleaning needle 41 is configured to clean the reaction cup 23 on the corresponding reaction disk 22 after completion of incubation, and the substrate needle 42 is configured to add a substrate to the reaction cup 23 on the corresponding reaction disk 22 after completion of incubation, which has been cleaned; a light shielding cover 43 which is capable of pivoting in a horizontal plane and ascending and descending in a vertical direction, is correspondingly arranged above the cleaning needle 41 and the substrate needle 42, and is used for shielding the cleaning needle 41, the substrate needle 42, the corresponding reaction tray 22 after incubation and all the reaction cups 23 on the reaction tray 22; and a detection head 44 disposed in the light shielding cover 43 and detecting the luminescence of the reactant in the reaction cuvette 23 to which the substrate is added. Wherein: the sample tray 12 and the reagent tray 14 of the sample supplying mechanism 1 rotate relatively, so that the corresponding sample tube 11 on the sample tray 12 and the corresponding reagent tube 13 on the reagent tray 14 are aligned in a radial direction and are located below the linear moving unit 31, the linear moving unit 31 drives the sample needle 32 and the reagent needle 33 to move linearly together to the upper side of the corresponding sample tube 11 and the corresponding reagent tube 13 respectively along the direction from the common circle center of the sample tray 12 and the reagent tray 14 of the sample supplying mechanism 1 to the circle center of the rotating disc 21 and align with the corresponding sample tube 11 and the corresponding reagent tube 13 respectively; thereafter, the linear moving unit 31 drives the sample needle 32 and the reagent needle 33 to move downward at the same time to suck the sample in the sample tube 11 and the reagent in the reagent tube 13, respectively; then the linear moving unit 31 drives the sample needle 32 and the reagent needle 33 to move upwards, and drives the sample needle 32 and the reagent needle 33 to move towards the rotary table 21 together along the direction from the common circle center of the sample disc 12 and the reagent disc 14 of the sample feeding mechanism 1 to the circle center of the rotary table 21; the driving mechanism 24 of the chemical reaction incubation mechanism 2 rotates the rotary plate 21, so that the plurality of reaction plates 22 revolve and one of the reaction plates 22 reaches the lower part of the linear movement unit 31 and the corresponding one of the reaction cups 23 on the reaction plate 22 reaches the sample application position under the linear movement unit 31; the linear moving unit 31 drives the sample needle 32 sucking the sample and the reagent needle 33 sucking the reagent to move above the cuvette 23 at the sample adding position along the direction from the common center of the sample plate 12 and the reagent plate 14 of the sample supply mechanism 1 to the center of the turntable 21, when the sample needs to be added, the sample needle 32 reaches above the cuvette 23 at the sample adding position, then the linear moving unit 31 drives the sample needle 32 sucking the sample and the reagent needle 33 sucking the reagent to move downwards simultaneously so as to add the sample in the sample needle 32 into the cuvette 23 at the sample adding position, when the reagent needs to be added, the reagent needle 33 reaches above the cuvette 23 at the sample adding position, and then the linear moving unit 31 drives the sample needle 32 and the reagent needle 33 sucking the reagent to move downwards simultaneously so as to add the reagent in the reagent needle 33 into the cuvette 23 at the sample adding position; after the reaction cups 23 of one reaction tray 22 at the sample application position complete the sample application and the reagent application, the reaction tray 22 rotates by an angle to rotate the other reaction cup 23 of the reaction tray 22 to the sample application position; the sample needle 32 completing the sample addition and the reagent needle 33 completing the reagent addition move in a direction opposite to a direction from the common center of the sample tray 12 and the reagent tray 14 of the sample mechanism 1 to the center of the rotary table 21 by the driving of the linear moving unit 31 and move above the cleaning groove 34; thereafter, the straight-line moving unit 31 drives the sample needle 32 where the sample addition is completed and the reagent needle 33 where the reagent addition is completed to move down into the cleaning bath 34 for cleaning; after the cleaning is completed, the linear moving unit 31 drives the sample needle 32 after the cleaning and the reagent needle 33 after the cleaning to move back upwards, and then the linear moving unit 31 drives the sample needle 32 after the back and the reagent needle 33 after the back to move towards the sample tray 12 and the reagent tray 14 of the sample supply mechanism 1 together, so as to perform the next sample suction and reagent suction and perform the reagent addition and the sample addition to the other reaction cup 23 of the reaction tray 22 at the sample application position; after all the cuvettes 23 in the reaction tray 22 in the sample application position complete the reagent application and the sample application, the driving mechanism 24 drives the rotating disc 21 to rotate by an angle, so as to make all the cuvettes 22 revolve by an angle, thereby making the reaction tray 22 shift and all the cuvettes 23 on the reaction tray 22 in the incubation state and making the other reaction tray 22 in the sample application position and making one cuvette 23 of the other reaction tray 22 in the sample application position, so as to perform the reagent application and the sample application; when the turntable 21 makes all the reaction disks 22 revolve, the light shielding cover 43 moves up pivotally to avoid the reaction disks 22 during revolution, when all the reaction disks 22 finish the revolution, each reaction disk 22 reaches a prescribed position, one of the reaction disks 22 reaches the sample addition position, and the corresponding reaction disk 22 in which the reaction cup 23 finishes incubation reaches below the position of the light shielding cover 43, the light shielding cover 43 moves down pivotally to shield the reaction disk 22, and then the reaction cup 23 is positioned below the cleaning needle 41 and the substrate needle 42 in sequence along with the rotation of the reaction disk 22, and the cleaning needle 41 moves down to add the cleaning solution into the reaction cup 23 and suck out the excess liquid, and the substrate needle 42 moves down to add the substrate into the reaction cup 23 along with the rotation of the reaction disk 22; finally, the reagent in the reaction cup 23 after the substrate is added is detected by the detection head 44 through luminescence.
It should be noted here that the chemical reaction incubation mechanism 2 is set to be at a constant temperature, which means that the whole incubation mechanism is set to be at a constant temperature region, that is, the reaction tray 22 and the reaction cup 23 are at a constant temperature, and the constant temperature requirement is satisfied that the incubation of the chemical reaction is started after the sample and the reagent are added into the reaction cup 23. The thermostating can be carried out by known addition means.
It is also noted herein that the order of adding the reagent and adding the sample is not limited, and the reagent may be added first and then the sample, or the sample may be added first and then the reagent may be added, and the linear moving unit 31 may be controlled to move differently regardless of the order.
It is also explained here that the linear moving unit 31 may employ any known mechanism.
In one embodiment, the sample tray 12 and the reagent tray 14 of the sample supplying mechanism 1 can rotate jointly, and the reagent can be uniformly mixed through forward and reverse rotation, so that the problem of noise caused by uniformly mixing by using other mechanisms such as a gear and rack transmission mechanism is solved.
Compare in the feed mechanism of prior art's guide tracked, the sample dish 12 and the reagent dish 14 of supply appearance mechanism 1 all adopt the ring shape and adopt chemical reaction to incubate carousel 21 of mechanism 2, a plurality of reaction discs 22 on the carousel 21 can rotation and revolution, greatly reduce the shared space of whole chemiluminescence immunoassay device, the detection flux has been improved greatly, owing to incubate on carousel 21, the setting of the processing station of incubate alone among the prior art has been cancelled, thereby greatly improved work efficiency.
By using the linear movement unit 31, the annular sample disk 12 and reagent disk 14, and the reaction disk 22 capable of rotating and revolving, high-precision positioning can be achieved.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1, the chemiluminescent immunoassay device may further comprise a storage mechanism 5. The storage mechanism 5 includes: a reaction tray magazine 51 for providing a reaction tray 22 to be used and reaction cups 23 on the reaction tray 22; and a sample tube storage 52 for accommodating a plurality of sample tubes 11 to be inspected and for receiving the sample tubes 11 subjected to sample suction. In an embodiment, referring to fig. 1, the reaction tray bin 51 and the sample tube bin 52 of the storage mechanism 5 may be integrated, the reaction tray bin 51 is circular corresponding to the size of the reaction tray 22, the sample tube bin 52 is annular and is disposed around the reaction tray bin 51, and the sample tube bin 52 is axially provided with a plurality of receiving parts 521 for receiving the sample tubes 11 to be detected and the sample tubes 11 for completing sample suction.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1 and 2, the sample supply mechanism 1 may further include: and a reaction tray recovery area 15, which is arranged at the central part of the sample tray 12 and the reagent tray 14 and is used for recovering the reaction tray 22 after the luminescence detection and all reaction cups 23 on the reaction tray 22.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1 and 3, a chucking hole 222 may be provided at the center of each reaction disk 22; the chemiluminescent immunoassay device may also include a handling mechanism 6. The carrying mechanism 6 may include: a guide rail 60; a slider 61 forming a slide pair with the guide rail 60 and movable in the vertical direction; a large arm 62 pivotally connected to the top of the slider 61, rotatable in a horizontal plane around the slider 61, and extending in the horizontal direction; a small arm 63 having one end pivotally connected to the large arm 62 and rotating in a horizontal plane about a pivot point with the large arm 62 and extending in a horizontal direction; a forceps 64 fixedly provided at the other end of the small arm 63 and extending downward; the electromagnet 65 is fixedly arranged on the tweezers 64, when the electromagnet 65 is powered on, the electromagnet 65 is attracted, the tail ends of the tweezers 64 are opened, and when the electromagnet 65 is powered off, the electromagnet 65 is opened, and the tail ends of the tweezers 64 are closed. Wherein: the slider 61 moves upward along the guide rail 60 and the large arm 62 and the small arm 63 pivotally move so that the tweezers 64 reach above the reaction disk 22 for which the luminescence detection has been completed; then the electromagnet 65 is powered off, the electromagnet 65 is opened, the tail ends of the tweezers 64 are closed, and the slide block 61 moves downwards along the guide rail 60 so that the tail ends of the tweezers 64 extend into the clamping holes 222 of the reaction disk 22 which has finished luminescence detection; then, when the electromagnet 65 is energized, the electromagnet 65 is attracted, and the end of the tweezers 64 is opened, so that the end of the tweezers 64 clamps the inner wall of the clamping hole 222 of the reaction disk 22 which has finished luminescence detection; thereafter, the slider 61 is moved upward along the guide rail 60 to take out the reaction disk 22, which has completed the luminescence detection, from the turntable 21, and the large arm 62 and the small arm 63 are pivotally moved to bring the reaction disk 22, which has completed the luminescence detection, held thereby above the reaction disk recovery area 15; then, the slide block 61 moves downwards along the guide rail 60 to the recovery area of the reaction cup 23, the electromagnet 65 is powered off, the electromagnet 65 is opened, the tail end of the tweezers 64 is closed, and the tail end of the tweezers 64 is separated from the inner wall of the clamping hole 222 of the reaction disc 22 which is finished with the luminescence detection; thereafter, the slider 61 moves upward along the guide rail 60 and the pivotal movement of the large arm 62 and the small arm 63 returns to the home position; the slider 61 moves downward along the guide rail 60 and the large arm 62 and the small arm 63 pivotally move so that the tweezers 64 reach above the reaction disk 22 to be used of the reaction disk magazine 51; then, the electromagnet 65 is powered off, the electromagnet 65 is opened, the tail ends of the tweezers 64 are closed, and the slide block 61 moves downwards along the guide rail 60, so that the tail ends of the tweezers 64 extend into the clamping holes 222 of the reaction disk 22 to be used; then, when the electromagnet 65 is energized, the electromagnet 65 is attracted, and the end of the tweezers 64 is opened, so that the end of the tweezers 64 clamps the inner wall of the clamping hole 222 of the reaction disk 22 to be used; thereafter, the slider 61 is moved upward along the guide rail 60 to take out the reaction disk 22 to be used from the reaction disk magazine 51, and the large arm 62 and the small arm 63 are pivotally moved to position the reaction disk 22 to be used held above the position where the transferred reaction disk 22 is vacant; the slide block 61 moves downwards along the guide rail 60 to place the reaction disk 22 to be used, which is clamped, in a vacant position of the reaction disk 22 transferred on the chemical reaction incubation mechanism 2; the electromagnet 65 is powered off, the electromagnet 65 is opened, the tail end of the tweezers 64 is closed, and the tail end of the tweezers 64 is separated from the inner wall of the clamping hole 222 of the reaction disc 22 to be used; thereafter, the slider 61 moves upward along the guide rail 60 and the pivotal movement of the large arm 62 and the small arm 63 returns to the home position.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1 and 3, the handling mechanism 6 is further capable of holding sample tubes 11 in the sample tube magazine 52 and transferring to the sample tray 12 of the sample presentation mechanism 1 and/or is further capable of holding sample tubes 11 of a completed sample presentation of the sample presentation mechanism 1 and transferring into the sample tube magazine 52.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1 and 2, the chemiluminescent immunoassay device may further include a sample information identification mechanism 7. The sample information identification mechanism 7 may include: and a camera 71 which is provided at a side of the sample supply mechanism 1, and when the sample tray 12 and the reagent tray 14 of the sample supply mechanism 1 are rotated to rotate the corresponding sample tube 11 and are exposed to the front of the camera 71, the camera 71 captures and collects label information on the sample tube 11.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 2, the sample tray 12 may be at the periphery of the reagent tray 14, with the camera 71 at the outside of the sample tray 12 and disposed facing the sample tubes 11 of the sample tray 12.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 2, the sample information identifying means 7 may further include: a support frame 72 for supporting the camera 71.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 5, the rotary disk 21 of the chemical reaction incubation mechanism 2 may be provided with a plurality of through holes 211 corresponding to the number of reaction disks 22, each through hole 211 being concentric with a corresponding one of the reaction disks 22; the chemical reaction incubation means 2 may further comprise: a plurality of connecting rods 25, the number of which corresponds to the number of the reaction trays 22, each connecting rod 25 passing through a corresponding through hole 211 and having one end detachably and fixedly connected to the bottom of a corresponding reaction tray 22; and a plurality of circular bases 26, the number of which corresponds to the number of the reaction trays 22, each circular base 26 being fixedly connected to the other end of a corresponding one of the connecting rods 25; the drive mechanism 24 of the chemical reaction incubation mechanism 2 may comprise: a motor 241; a main gear 242 power-connected to the motor 241; a plurality of pinions 243 corresponding in number to the number of reaction disks 22; a plurality of pulleys 244 corresponding in number to the pinions 243, each pulley 244 being coaxially connected to a corresponding one of the pinions 243; a plurality of belts 245, the number of which corresponds to the number of pulleys 244, each belt 245 being wound around a corresponding one of the pulleys 244 and a corresponding one of the circular bases 26; and an electromagnetic clutch 246 fixedly connected to a bottom of the turntable 21 and adapted to be engaged with or disengaged from the main gear 242 so as to disengage the plurality of pinions 243 from the main gear 242 or from the main gear 242; wherein: when the electromagnetic clutch 246 is engaged with the main gear 242, the main gear 242 is disengaged from the plurality of pinions 243, and the motor 241 drives the turntable 21 to rotate through the main gear 242, the electromagnetic clutch 246 and the bottom of the turntable 21, so as to realize the revolution of all the reaction disks 22 on the turntable 21; when the electromagnetic clutch 246 is disengaged from the main gear 242, the main gear 242 is engaged with the plurality of pinions 243, and the motor 241 drives the plurality of reaction disks 22 to rotate simultaneously through the main gear 242, the plurality of pinions 243, the plurality of pulleys 244, the plurality of belts 245, the circular bases 26 of the plurality of reaction disks 22, and the plurality of connecting rods 25. In one embodiment, the removably secured connection may be a socket.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1 and 4, the washing needle 41 may include: cleaning the needle 411, adding a cleaning solution into the reaction cup 23; and cleaning the suction needle 412 to suck out the excess liquid in the reaction cup 23. In one embodiment, referring to fig. 1 and 4, the number of the cleaning needles 41 is 5, the number of the substrate needles 42 is 2, and the cleaning needles 411, the cleaning suction needles 412, the cleaning suction needles 411, the cleaning suction needles 412, the cleaning suction needles 42, and the substrate needles 42 are arranged in this order.
In one embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1, 4 and 5, the reaction disk 22 is 4 and is arranged at equal intervals along the circumference of the rotating disk 21.
In an embodiment of the chemiluminescent immunoassay device according to the present invention, referring to fig. 1 and 2, the holding part 141 on the reagent disk 14 is provided in two circles and two holding parts 141 are present in the same radial direction on the holding part 141 on the two circles to store two reagents for detecting one sample along the same radial line, and for this case, the linear motion unit 31 may simultaneously drive the sample needle 32 to suck the sample and the reagent needle 33 to suck the reagent, and the sample needle 32 and the reagent needle 33 may simultaneously move up and down. After the sample needle 32 sucks the sample and the reagent needle 33 sucks the reagent, the sample needle 32 and the reagent needle 33 are simultaneously moved in the direction of the reaction disk 22 by the driving of the linear motion unit 31, and when they reach the upper side of one cuvette 23 of the reaction disk 22 at the sample adding position, one reagent may be added first, the sample may be added, and the second reagent may be added last by the reciprocating driving of the linear motion unit 31.

Claims (10)

1. A chemiluminescent immunoassay device comprising:
a sample supply mechanism (1);
the chemical reaction incubation mechanism (2) is set to be constant in temperature;
a sample adding mechanism (3);
a light emission detection means (4);
it is characterized in that the preparation method is characterized in that,
the sample supply mechanism (1) comprises:
a plurality of sample tubes (11), each sample tube (11) being for containing a sample to be tested;
the sample disc (12) is annular and can rotate, and an accommodating cavity (121) for accommodating the sample tube (11) is arranged along the circumferential direction of the sample disc (12);
a plurality of reagent tubes (13), each reagent tube (13) for holding a reagent; and
the reagent disk (14) is annular and can rotate, is concentrically arranged at the inner side of the sample disk (12), and is provided with a containing part (141) for containing the reagent tube (13) along the circumferential direction;
the chemical reaction incubation mechanism (2) comprises:
a rotary plate (21) which is circular and is opposite to the sample plate (12) and the reagent plate (14);
a plurality of reaction discs (22) which are arranged on the rotating disc (21) at equal intervals along the circumferential direction of the rotating disc (21), and reaction cup accommodating cavities (221) are arranged on each reaction disc (22) at equal intervals along the circumferential direction;
a plurality of reaction cups (23) which are arranged at equal intervals along the circumferential direction of each reaction disk (22), and each reaction cup (23) is inserted into and held in one reaction cup accommodating cavity (221) on the corresponding reaction disk (22); and
the driving mechanism (24) is in power connection with the rotating disc (21) and the plurality of reaction discs (22) so as to enable the rotating disc (21) to rotate to drive all the reaction discs (22) to revolve together or enable all the reaction discs (22) to rotate independently;
the sample adding mechanism (3) comprises:
the linear moving unit (31) is arranged above the sample disc (12) and the reagent disc (14) of the sample feeding mechanism (1) and the rotary disc (21) along the direction from the common circle center of the sample disc (12) and the reagent disc (14) of the sample feeding mechanism (1) to the circle center of the rotary disc (21);
the sample needle (32) is in power connection with the linear moving unit (31), can be driven by the linear moving unit (31) to linearly reciprocate and can move up and down along the direction from the common circle center of the sample disc (12) and the reagent disc (14) of the sample supplying mechanism (1) to the circle center of the rotating disc (21) so as to suck a sample from a sample tube (11) accommodated on the sample disc (12) and add the sample to a reaction cup (23) on a reaction disc (22) at a sample adding position below the linear moving unit (31);
the reagent needle (33) is fixedly connected with the sample needle (32) at an interval and is in power connection with the linear moving unit (31), can be driven by the linear moving unit (31) to linearly reciprocate and can move up and down along the direction from the common circle center of the sample disk (12) and the reagent disk (14) of the sample feeding mechanism (1) to the circle center of the rotating disk (21), so as to suck a reagent from a reagent tube (13) accommodated on the reagent disk (14) and add the reagent into a reaction cup (23) on a reaction disk (22) at a sample feeding position below the linear moving unit (31); and
a cleaning tank (34) which is located below the linear moving unit (31) and between the sample feeding mechanism (1) and the chemical reaction incubation mechanism (2) and is used for cleaning a sample needle (32) for completing sample addition and a reagent needle (33) for completing reagent addition;
the light emission detection mechanism (4) includes:
the cleaning needle (41) and the substrate needle (42) are arranged above one corresponding reaction disc (22), are sequentially arranged along the circumferential direction of the reaction disc (22) in a manner of aligning with one part of a plurality of reaction cups (23) which are circumferentially arranged on the reaction disc (22), and can move along the up-down direction respectively, the cleaning needle (41) is used for cleaning the reaction cups (23) on the corresponding reaction disc (22) which is incubated to be finished, and the substrate needle (42) is used for adding substrates to the reaction cups (23) which are incubated to be finished and cleaned and are arranged on the corresponding reaction disc (22);
a light shielding cover (43) which can pivot in the horizontal plane and can lift in the vertical direction, is correspondingly arranged above the cleaning needle (41) and the substrate needle (42), and is used for shielding the cleaning needle (41), the substrate needle (42), the corresponding reaction disc (22) after incubation and all reaction cups (23) on the reaction disc (22); and
a detection head (44) which is arranged in the light shielding cover (43) and is used for carrying out luminescence detection on the reactant in the reaction cup (23) added with the substrate;
wherein:
the sample tray (12) and the reagent tray (14) of the sample supply mechanism (1) relatively rotate, so that corresponding sample tubes (11) on the sample tray (12) and corresponding reagent tubes (13) on the reagent tray (14) are aligned in the radial direction and are located below the linear moving unit (31), the linear moving unit (31) drives the sample needles (32) and the reagent needles (33) to linearly move to the positions above the corresponding sample tubes (11) and reagent tubes (13) along the direction from the common circle center of the sample tray (12) and the reagent tray (14) of the sample supply mechanism (1) to the circle center of the rotary table (21) and respectively align with the corresponding sample tubes (11) and reagent tubes (13); then, the linear moving unit (31) drives the sample needle (32) and the reagent needle (33) to move downwards simultaneously so as to suck the sample in the sample tube (11) and the reagent in the reagent tube (13) respectively; then the linear moving unit (31) drives the sample needle (32) and the reagent needle (33) to move upwards, and drives the sample needle (32) and the reagent needle (33) to move towards the rotary table (21) together along the direction from the common circle center of the sample disc (12) and the reagent disc (14) of the sample feeding mechanism (1) to the circle center of the rotary table (21);
the driving mechanism (24) of the chemical reaction incubation mechanism (2) drives the rotating disc (21) to rotate, so that the reaction discs (22) revolve and one reaction disc (22) reaches the lower part of the linear moving unit (31) and the corresponding reaction cup (23) on the reaction disc (22) is positioned below the linear moving unit (31) to reach the sample adding position;
the linear moving unit (31) drives the sample needle (32) for sucking the sample and the reagent needle (33) for sucking the reagent to move towards the upper part of the reaction cup (23) at the sample adding position along the direction from the common circle center of the sample disc (12) and the reagent disc (14) of the sample supplying mechanism (1) to the circle center of the turntable (21), when the sample needs to be added, the sample needle (32) reaches the upper part of the reaction cup (23) at the sample adding position, then the linear moving unit (31) drives the sample needle (32) for sucking the sample and the reagent needle (33) for sucking the reagent to simultaneously move downwards so as to add the sample in the sample needle (32) into the reaction cup (23) at the sample adding position, when the reagent needs to be added, the reagent needle (33) reaches the upper part of the reaction cup (23) at the sample adding position, then the linear moving unit (31) drives the sample needle (32) and the reagent needle (33) for sucking the reagent to simultaneously move downwards, so as to add the reagent in the reagent needle (33) into the reaction cup (23) at the sample adding position;
when the reaction cups (23) of one reaction plate (22) at the sample adding position complete the adding of the sample and the adding of the reagent, the reaction plate (22) rotates at an angle, and the other reaction cup (23) of the reaction plate (22) rotates to the sample adding position;
the sample needle (32) completing the sample addition and the reagent needle (33) completing the reagent addition move along the direction opposite to the direction from the common circle center of the sample disc (12) and the reagent disc (14) of the sample supplying mechanism (1) to the circle center of the rotating disc (21) under the driving of the linear moving unit (31) and move to the upper part of the cleaning groove (34); then, the straight moving unit (31) drives the sample needle (32) where the sample addition is completed and the reagent needle (33) where the reagent addition is completed to move down into the cleaning tank (34) for cleaning; after the cleaning is finished, the linear moving unit (31) drives the sample needle (32) which is finished to be cleaned and the reagent needle (33) which is finished to be cleaned to move upwards to return, and then the linear moving unit (31) drives the returned sample needle (32) and the returned reagent needle (33) to move towards the sample tray (12) and the reagent tray (14) of the sample feeding mechanism (1) together so as to perform next sample suction and reagent suction and perform reagent addition and sample addition on the other reaction cup (23) of the reaction tray (22) at the sample feeding position;
after all reaction cups (23) in the reaction tray (22) at the sample adding position complete the adding of the reagent and the adding of the sample, the driving mechanism (24) drives the rotating disc (21) to rotate for an angle so as to make all reaction trays (22) revolve for an angle, thereby making the reaction tray (22) shift and all reaction cups (23) on the reaction tray (22) in an incubation state and making the other reaction tray (22) at the sample adding position and making one reaction cup (23) of the other reaction tray (22) rotate to the sample adding position so as to add the reagent and the sample;
when the turntable (21) makes all the reaction disks (22) revolve, the light shielding cover (43) pivots upwards to avoid the reaction disks (22) during revolution, when the revolution of all the reaction disks (22) is finished, each reaction disk (22) reaches a specified position, one reaction disc (22) reaches the sample adding position, the reaction cup (23) finishes incubation, the corresponding reaction disc (22) reaches the position below the position of the light shielding cover (43), the light shielding cover (43) pivots and moves downwards to shield the reaction disc (22), and then the reaction cup (23) is sequentially positioned below the cleaning needle (41) and the substrate needle (42) along with the rotation of the reaction disc (22), and with the rotation of the reaction disk (22), the cleaning needle (41) moves downwards to add the cleaning liquid into the reaction cup (23) and suck the redundant liquid out, and the substrate needle (42) moves downwards to add the substrate into the reaction cup (23); finally, the reaction substance in the reaction cup (23) added with the substrate is detected by the detection head (44) through luminescence.
2. The chemiluminescent immunoassay device of claim 1 further comprising:
a storage mechanism (5) comprising:
a reaction tray magazine (51) for providing a reaction tray (22) to be used and reaction cups (23) on the reaction tray (22); and
and a sample tube storage bin (52) for providing a plurality of sample tubes (11) to be detected and accommodating the sample tubes (11) with sample suction completed.
3. The chemiluminescent immunoassay device according to claim 2, wherein the reaction tray magazine (51) and the sample tube magazine (52) of the storage mechanism (5) are integrated, the reaction tray magazine (51) is circular corresponding to the size of the reaction tray (22), the sample tube magazine (52) is annular and is arranged around the reaction tray magazine (51), and the sample tube magazine (52) is axially provided with a plurality of receiving parts (521) for receiving sample tubes (11) to be tested and sample tubes (11) for completing sample suction.
4. The chemiluminescent immunoassay device of claim 2 wherein the sample presentation mechanism (1) further comprises:
and the reaction disc recovery area (15) is arranged at the central parts of the sample disc (12) and the reagent disc (14) and is used for recovering the reaction disc (22) after the luminescence detection and all reaction cups (23) on the reaction disc (22).
5. The chemiluminescent immunoassay device of claim 4,
a clamping hole (222) is arranged at the center of each reaction disk (22);
the chemiluminescent immunoassay device further comprises:
the carrying mechanism (6) comprises:
a guide rail (60);
a slider (61) which forms a sliding pair with the guide rail (60) and can move in the vertical direction on the guide rail (60);
a large arm (62) which is pivoted to the top of the sliding block (61), can rotate around the sliding block (61) in a horizontal plane and extends along the horizontal direction;
a small arm (63) having one end pivotally connected to the large arm (62) and rotating in a horizontal plane about a pivot point with the large arm (62) and extending in a horizontal direction;
a forceps (64) fixedly arranged at the other end of the small arm (63) and extending downwards;
the electromagnet (65) is fixedly arranged on the tweezers (64), when the electromagnet (65) is electrified, the electromagnet (65) is attracted, the tail end of the tweezers (64) is opened, and when the electromagnet (65) is powered off, the electromagnet (65) is opened, and the tail end of the tweezers (64) is closed;
wherein:
the slide block (61) moves upwards along the guide rail (60) and the large arm (62) and the small arm (63) pivot to enable the tweezers (64) to reach the upper part of the reaction disc (22) which completes the luminescence detection; then the electromagnet (65) is powered off, the electromagnet (65) is opened, the tail ends of the tweezers (64) are closed, and the sliding block (61) moves downwards along the guide rail (60) so that the tail ends of the tweezers (64) extend into the clamping holes (222) of the reaction disc (22) which finishes the luminescence detection; then, when the electromagnet (65) is electrified, the electromagnet (65) is attracted, the tail ends of the tweezers (64) are opened, so that the tail ends of the tweezers (64) clamp the inner wall of the clamping hole (222) of the reaction disc (22) which finishes the luminescence detection; then, the slide block (61) moves upwards along the guide rail (60) to take the reaction disk (22) which has finished luminescence detection out of the turntable (21), and the large arm (62) and the small arm (63) pivotally move to enable the clamped reaction disk (22) which has finished luminescence detection to reach the upper part of the reaction disk recovery area (15); then, the slide block (61) moves downwards along the guide rail (60) to the recovery area of the reaction cup (23), the electromagnet (65) is powered off, the electromagnet (65) is opened, the tail end of the forceps (64) is closed, and the tail end of the forceps (64) is separated from the inner wall of the clamping hole (222) of the reaction disc (22) which has finished luminescence detection; then, the slide block (61) moves upwards along the guide rail (60) and the pivoting motion of the large arm (62) and the small arm (63) returns to the original position;
the slide block (61) moves downwards along the guide rail (60) and the large arm (62) and the small arm (63) pivot to enable the tweezers (64) to reach the upper part of the reaction disk (22) to be used of the reaction disk storage bin (51); then, the electromagnet (65) is powered off, the electromagnet (65) is opened, the tail ends of the tweezers (64) are closed, and the sliding block (61) moves downwards along the guide rail (60) so that the tail ends of the tweezers (64) extend into the clamping holes (222) of the reaction disc (22) to be used; then, when the electromagnet (65) is electrified, the electromagnet (65) is attracted, the tail ends of the tweezers (64) are opened, so that the tail ends of the tweezers (64) clamp the inner wall of the clamping hole (222) of the reaction disc (22) to be used; then, the slide block (61) moves upwards along the guide rail (60) to take the reaction disk (22) to be used out of the reaction disk storage bin (51), and the large arm (62) and the small arm (63) pivotally move to enable the clamped reaction disk (22) to be used to be positioned above the position where the transferred reaction disk (22) is vacant; the slide block (61) moves downwards along the guide rail (60) to place the clamped reaction disc (22) to be used on the vacant position of the transferred reaction disc (22) on the chemical reaction incubation mechanism (2); the electromagnet (65) is powered off, the electromagnet (65) is opened, the tail end of the tweezers (64) is closed, and the tail end of the tweezers (64) is separated from the inner wall of the clamping hole (222) of the reaction disc (22) to be used; thereafter, the slider (61) moves upward along the guide rail (60) and the pivotal movement of the large arm (62) and the small arm (63) returns to the home position.
6. The chemiluminescent immunoassay device of claim 5,
the moving mechanism (6) can also hold sample tubes (11) in the sample tube storage (52) and transfer the sample tubes to the sample plate (12) of the sample supply mechanism (1) and/or can also hold sample tubes (11) of the sample supply mechanism (1) which have finished providing samples and transfer the sample tubes to the sample tube storage (52).
7. The chemiluminescent immunoassay device of claim 1 further comprising:
sample information identification means (7) comprising:
and the camera (71) is arranged on the side of the sample feeding mechanism (1), and when the sample tray (12) and the reagent tray (14) of the sample feeding mechanism (1) rotate to enable the corresponding sample tube (11) to rotate and be exposed in front of the camera (71), the camera (71) shoots and collects label information on the sample tube (11).
8. The chemiluminescent immunoassay device of claim 7, wherein the sample tray (12) is at the periphery of the reagent tray (14) and the camera (71) is at the outside of the sample tray (12) and is disposed facing the sample tubes (11) of the sample tray (12).
9. The chemiluminescent immunoassay device of claim 5,
the rotary disc (21) of the chemical reaction incubation mechanism (2) is provided with a plurality of through holes (211), the number of the through holes corresponds to that of the reaction discs (22), and each through hole (211) is concentric with one corresponding reaction disc (22);
the chemical reaction incubation mechanism (2) further comprises:
the number of the connecting rods (25) corresponds to that of the reaction discs (22), and each connecting rod (25) penetrates through one corresponding through hole (211) and one end of each connecting rod is detachably and fixedly connected to the bottom of one corresponding reaction disc (22); and
the number of the circular bases (26) corresponds to that of the reaction discs (22), and each circular base (26) is fixedly connected to the other end of one corresponding connecting rod (25);
the drive mechanism (24) of the chemical reaction incubation mechanism (2) comprises:
a motor (241);
a main gear (242) which is power connected with the motor (241);
a plurality of pinions (243) corresponding in number to the number of reaction disks (22);
a plurality of pulleys (244) corresponding in number to the number of the pinions (243), each pulley (244) being coaxially connected to a corresponding one of the pinions (243);
a plurality of belts (245) corresponding in number to the pulleys (244), each belt (245) being wound around a respective one of the pulleys (244) and a respective one of the circular bases (26); and
an electromagnetic clutch (246) fixedly connected to the bottom of the turntable (21) and adapted to engage with or disengage from the main gear (242) to disengage the plurality of pinions (243) from the main gear (242) or from the main gear (242);
wherein:
when the electromagnetic clutch (246) is engaged with the main gear (242), the main gear (242) is disengaged from the plurality of pinions (243), and the motor (241) drives the rotating disc (21) to rotate through the main gear (242), the electromagnetic clutch (246) and the bottom of the rotating disc (21) so as to realize the revolution of all reaction discs (22) on the rotating disc (21);
when the electromagnetic clutch (246) is disengaged from the main gear (242), the main gear (242) is engaged with the plurality of pinions (243), and the motor (241) drives the plurality of reaction disks (22) to rotate simultaneously through the main gear (242), the plurality of pinions (243), the plurality of pulleys (244), the plurality of belts (245), the circular bases (26) of the plurality of reaction disks (22), and the plurality of connecting rods (25).
10. The chemiluminescent immunoassay device of claim 1 wherein the cleaning needle (41) comprises:
a cleaning needle (411) for adding a cleaning liquid into the reaction cup (23); and
the suction needle (412) is cleaned to suck out the excessive liquid in the reaction cup (23).
CN201510181011.9A 2015-04-16 2015-04-16 Chemiluminescence immune assay device Expired - Fee Related CN104777321B (en)

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