CN109580594B - Chemiluminescent detector, incubation device and reaction disk mechanism - Google Patents
Chemiluminescent detector, incubation device and reaction disk mechanism Download PDFInfo
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- CN109580594B CN109580594B CN201710903155.XA CN201710903155A CN109580594B CN 109580594 B CN109580594 B CN 109580594B CN 201710903155 A CN201710903155 A CN 201710903155A CN 109580594 B CN109580594 B CN 109580594B
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 835
- 230000007246 mechanism Effects 0.000 title claims abstract description 144
- 238000011534 incubation Methods 0.000 title claims abstract description 96
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 154
- 238000002156 mixing Methods 0.000 claims abstract description 72
- 230000000670 limiting effect Effects 0.000 claims abstract description 51
- 230000033001 locomotion Effects 0.000 claims abstract description 29
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000003825 pressing Methods 0.000 claims description 66
- 238000005096 rolling process Methods 0.000 claims description 55
- 230000005540 biological transmission Effects 0.000 claims description 35
- 230000006835 compression Effects 0.000 claims description 33
- 238000007906 compression Methods 0.000 claims description 33
- 230000001360 synchronised effect Effects 0.000 claims description 33
- 238000005461 lubrication Methods 0.000 claims description 29
- 238000012546 transfer Methods 0.000 claims description 28
- 238000004140 cleaning Methods 0.000 claims description 24
- 238000009434 installation Methods 0.000 claims description 17
- 238000005056 compaction Methods 0.000 claims description 16
- 230000002093 peripheral effect Effects 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 5
- 238000010926 purge Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- 230000000875 corresponding effect Effects 0.000 description 45
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- 238000011161 development Methods 0.000 description 5
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- 238000012545 processing Methods 0.000 description 5
- 230000003139 buffering effect Effects 0.000 description 4
- 230000000977 initiatory effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000504 luminescence detection Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
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- 239000012535 impurity Substances 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 239000010687 lubricating oil Substances 0.000 description 1
- 239000000891 luminescent agent Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- 230000002035 prolonged effect Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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Abstract
The invention provides a reaction disk mechanism, comprising: a reaction disk mounting structure; the reaction disc driving structure is arranged on the reaction disc mounting structure; the reaction bearing disc is arranged on the reaction disc driving structure, and the reaction disc driving structure can drive the reaction bearing disc to do rotary motion; the reaction bearing disc can accommodate a reaction cup with a sample added, and can enable the reaction cup to finish the operation of adding the reagent and the operation of uniformly mixing on the reaction bearing disc; and the guiding and limiting structure can conduct guiding and limiting on the rotary motion of the reaction bearing disc. The occupied space of the reaction disc mechanism is reduced, and meanwhile, the cost is reduced; the invention also provides a chemiluminescent detector and an incubation device, when the reaction disc mechanism is applied to the incubation device, the problem of long running time caused by time occupation of each working procedure of the current sample reaction can be solved, the detection speed of the chemiluminescent detector is improved, and the detection efficiency is further ensured.
Description
Technical Field
The invention relates to the technical field of chemiluminescent detection, in particular to a chemiluminescent detector, an incubation device and a reaction disc mechanism.
Background
The chemiluminescent immunoassay is a non-radiolabeled immunoassay established on the basis of the theory of the radioimmunoassay technology and using a labeled luminescent agent as a tracer signal, and is widely carried out in the research and application of the contemporary biological detection technology. The kit has the advantages of high specificity, high sensitivity, simple separation, no toxicity, safety and stability, no environmental pollution and the like, and particularly can obtain experimental results in a short time, thus being deeply favored by test medical workers and clinicians.
At present, chemiluminescent detectors based on biochemical immunoassay have become established medical diagnostic devices. However, the universal chemiluminescent detector has the disadvantages of high price, heavy volume, large power consumption and difficult popularization and promotion. With the high-speed development of biomedical equipment, the full automation of the chemiluminescent detector is realized under certain conditions.
The chemiluminescent detector mainly comprises a reaction cup loading device, a sample adding device, an incubation reaction device, a cleaning device, a luminescent detection device, a control system and a software system. Typically, the incubation reaction apparatus is in an intermediate position and serves as a single station for incubating the reactants in the reaction vessel, and the incubation reaction apparatus is used to perform the incubation operations, and the various steps prior to incubation, such as adding the reaction cup, adding the sample, adding the reagents, and mixing, etc., need to be performed on other structures. At present, each section of working procedures of sample reaction such as adding a reaction cup, adding a sample, adding a reagent, mixing uniformly, incubating and the like are distributed in a production line when being executed, so that each section of working procedures occupy time and larger space when being executed, the cost is higher, mutual interference can occur, the parallel working cannot be performed at the same time, the efficiency is reduced, and the high-speed and miniaturized development of an instrument is seriously restricted.
Disclosure of Invention
In view of the above, it is necessary to provide a reaction tray mechanism capable of reducing the space occupation and the cost, and an incubator having the reaction tray mechanism, and a chemiluminescent detector having the incubator, in order to solve the problems of large space occupation and high cost caused by the block execution of the incubation reaction apparatus.
The above purpose is achieved by the following technical scheme:
a reaction disk mechanism comprising:
a reaction disk mounting structure;
the reaction disc driving structure is arranged on the reaction disc mounting structure;
the reaction bearing disc is arranged on the reaction disc driving structure, and the reaction disc driving structure can drive the reaction bearing disc to do rotary motion; the reaction bearing disc can accommodate reaction cups; and
The guiding and limiting structure can conduct guiding and limiting on the rotary motion of the reaction bearing disc.
In one embodiment, the guiding and limiting structure comprises a guiding and limiting sliding rail and a rolling support piece, wherein the guiding and limiting sliding rail is arranged on the reaction bearing disc, and the guiding and limiting sliding rail protrudes out of the outer peripheral surface of the reaction bearing disc;
the rolling support piece is arranged on the reaction disc mounting structure and positioned on the periphery of the reaction bearing disc, and the guide limiting sliding rail is slidably matched with the rolling support piece.
In one embodiment, the rolling support is a guiding limit bearing, a sliding groove is formed in an outer ring of the guiding limit bearing, and the guiding limit sliding rail is slidably located in the sliding groove.
In one embodiment, the cross section of the part of the guiding limit sliding rail located in the sliding groove is V-shaped, and the cross section of the sliding groove is V-shaped.
In one embodiment, the cross-sectional shape of the portion of the guiding and limiting sliding rail located in the sliding groove is matched with the cross-sectional shape of the sliding groove.
In one embodiment, the guiding and limiting structure further comprises a lubricating component, the lubricating component is arranged on the reaction disc mounting structure and located on the periphery side of the reaction bearing disc, and the guiding and limiting sliding rail can be in contact with the lubricating component.
In one embodiment, the lubrication part is provided with a lubrication groove, the guiding limit sliding rail is positioned in the lubrication groove, and the section shape of the lubrication groove is also V-shaped.
In one embodiment, the number of rolling supports is at least three, the at least three rolling supports being evenly distributed around the circumference of the reaction-carrying disk.
In one embodiment, the reaction plate mechanism further comprises a pressing structure, the pressing structure is arranged on the reaction plate mounting structure, one of the rolling support pieces is mounted on the pressing structure, and the pressing structure is used for adjusting the distance between the rolling support piece and the guiding limiting sliding rail.
In one embodiment, the pressing structure comprises a pressing installation seat, a pressing elastic piece and a pressing guide rod, wherein the pressing guide rod is installed on the reaction plate installation structure, the pressing installation seat is slidably arranged on the pressing guide rod, the pressing elastic piece is sleeved on the pressing guide rod, and two ends of the pressing elastic piece are respectively abutted to the pressing guide rod and the pressing installation seat;
the rolling support piece is installed on the compaction installation seat, and the compaction elastic piece can enable the compaction installation seat to move on the compaction guide rod and be abutted to the guide limiting sliding rail.
In one embodiment, the pressing structure further comprises a pressing fixing seat, the pressing fixing seat is fixed on the reaction plate mounting structure, and the pressing guide rod is mounted on the pressing fixing seat.
In one embodiment, the reaction carrying tray is provided with a plurality of placing holes for placing the reaction cups, the placing holes are uniformly distributed along the circumferential direction of the reaction carrying tray, and the reaction cups with added samples can be respectively transferred into the placing holes.
In one embodiment, the reaction tray mounting structure is provided with a plurality of reaction stations, including a cup adding station, a reagent adding station, a mixing station and an incubation cup taking station which are sequentially arranged;
the cup adding station is arranged corresponding to a cup grabbing mechanism, and the cup grabbing mechanism can place the reaction cup with the added sample into the placing hole of the reaction bearing disc at the cup adding station;
the reagent adding station is arranged corresponding to a reagent adding device, and the reagent adding device can add reagent into the reaction cup of the reaction bearing disc in the reagent adding station;
the mixing station is arranged corresponding to a mixing device, and the mixing device can mix samples in the reaction cup of the reaction bearing disc with reagents uniformly in the mixing station;
the incubation cup taking station is arranged corresponding to the cup grabbing mechanism, and the cup grabbing mechanism can grab the reaction cups in the reaction bearing disc at the incubation cup taking station and transfer the reaction cups to the reaction disc mechanism.
In one embodiment, the reaction station further comprises a cleaning and cup placing station, and the cleaning and cup placing station is located between the cup adding station and the reagent adding station.
In one embodiment, the reagent adding station comprises a first reagent adding station and a second reagent adding station, and the first reagent adding station and the second reagent adding station are positioned between the cleaning cup placing station and the mixing station.
In one embodiment, the reaction disk mounting structure further has a buffer station, the buffer station is located between any two adjacent reaction stations, and the buffer station can make the distances between two adjacent reaction stations and the buffer station equal.
In one embodiment, the reaction disk mounting structure further comprises a buffer station, wherein the buffer station comprises a first buffer station, a second buffer station and a third buffer station, and the cup adding station, the first buffer station, the cleaning and cup placing station, the second buffer station, the first reagent adding station, the second reagent adding station, the mixing station, the incubation cup taking station and the third buffer station are sequentially arranged at equal intervals along the circumferential direction of the reaction bearing disk.
In one embodiment, the number of the placement holes on the reaction carrying tray is an integer multiple of the sum of the number of the reaction stations and the buffer stations.
In one embodiment, the reaction disk driving structure comprises a reaction driving motor and a reaction transmission assembly, wherein the reaction driving motor is arranged on the reaction disk mounting structure, the reaction transmission assembly is in transmission connection with the output end of the reaction driving motor and the reaction bearing disk, and the reaction driving motor drives the reaction transmission assembly to drive the reaction bearing disk to rotate;
the reaction transmission assembly comprises a reaction driving wheel and a reaction synchronous belt, the reaction driving wheel is arranged at the output end of the reaction driving motor, the reaction synchronous belt is sleeved on the reaction driving wheel and the reaction bearing disc, the driving motor drives the reaction driving wheel to rotate, and the reaction bearing disc is driven to rotate through the reaction synchronous belt.
In one embodiment, the reaction plate mechanism further comprises a tensioning structure, the tensioning structure is arranged on the reaction plate mounting structure, and the tensioning structure is abutted to the reaction synchronous belt.
In one embodiment, the tensioning structure comprises a tensioning wheel, a tensioning wheel shaft, a tensioning guide rod and a tensioning elastic piece, wherein the tensioning guide rod is fixed on the reaction disc mounting structure, the tensioning wheel is rotatably arranged on the tensioning wheel shaft, the tensioning wheel is located on the outer side of the reaction synchronous belt, one end of the tensioning elastic piece is fixed on the tensioning guide rod, the other end of the tensioning elastic piece is connected with the tensioning wheel shaft, and the tensioning elastic piece can move along the tensioning guide rod to enable the tensioning wheel to be in butt joint with the reaction synchronous belt.
In one embodiment, the tensioning structure further comprises a tensioning sliding rail and a tensioning sliding block, the tensioning sliding rail is arranged on the reaction disc mounting structure, the tensioning wheel shaft is fixed on the tensioning sliding block, the tensioning sliding block is slidably arranged on the tensioning sliding rail, the tensioning elastic piece is connected with the tensioning sliding block, and the tensioning elastic piece can drive the tensioning sliding block to move along the tensioning sliding rail and drive the tensioning wheel shaft and the tensioning wheel on the tensioning wheel shaft to move.
In one embodiment, the tensioning structure further comprises a tensioning connecting component, the tensioning connecting component is connected with the tensioning sliding block and the tensioning elastic piece, and the tensioning elastic piece drives the tensioning sliding block to move through the tensioning connecting component.
An incubation apparatus comprising a buffer tray mechanism, a reaction inner tray mechanism and a reaction tray mechanism according to any of the technical features described above;
the buffer disc mechanism comprises a rotatable buffer bearing disc, wherein the buffer bearing disc can accommodate a reaction cup and can enable the reaction cup to finish sample adding operation on the buffer bearing disc;
the reaction bearing disc of the reaction disc mechanism can accommodate the reaction cup transferred from the buffer disc mechanism and can enable the reaction cup to finish the reagent adding operation and the uniform mixing operation on the reaction disc mechanism;
the reaction inner disc mechanism comprises a rotatable reaction inner disc bearing disc, wherein the reaction inner disc bearing disc can contain the reaction cup transferred from the reaction bearing disc and can enable the reaction cup to perform incubation operation on the reaction inner disc mechanism;
the reaction bearing disc and the reaction inner disc bearing disc can rotate independently.
In one embodiment, the reaction bearing disc is circular, and the reaction inner disc bearing disc is disc-shaped;
the reaction bearing disc is sleeved on the outer side of the reaction inner disc bearing disc, and the buffer bearing disc is independent of the reaction bearing disc.
The chemiluminescent detector comprises a reaction cup automatic transmission device, a sample adding device, a cleaning device, a luminescent detection device, a control device and an incubation device according to any technical characteristic;
the control device transfers the reaction cups in the automatic reaction cup conveying device to the incubation device, controls the sample adding device to add samples to the reaction cups in the incubation device, and controls the reaction cups to be sequentially transferred to the cleaning device and the light-emitting detection device after incubation is completed.
After the technical scheme is adopted, the beneficial effects of the invention are as follows:
according to the chemiluminescent detector, the incubation device and the reaction disk mechanism, the reaction bearing disk of the reaction disk mechanism can play a role of bearing the reaction cup after sample addition, and the reaction disk driving structure can drive the reaction bearing disk to rotate, so that the reaction bearing disk drives the reaction cup to finish reagent addition and uniform mixing operation, meanwhile, the guide limiting device plays a guide limiting role on the movement of the reaction bearing disk, the reagent addition and uniform mixing operation of the reaction cup is realized through the cooperation of the guide limiting structure and the reaction bearing disk, the problems of large occupied space, high cost and low efficiency of the existing execution structure are effectively solved, the occupied space is reduced, the cost is reduced, the efficiency is improved, and the instrument is beneficial to high-speed and miniaturized development; moreover, when the reaction disk mechanism is applied to the incubation device, the problem of long operation time caused by the occupied time and the occupied large space of each section of working procedure of the current sample reaction can be solved, the detection speed of the chemiluminescent detector is improved, and the detection efficiency is further ensured.
Drawings
FIG. 1 is a schematic view showing the structure of an incubator according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a reaction tray mechanism and a reaction inner tray mechanism in the incubation apparatus shown in FIG. 1;
FIG. 3 is a schematic view of the reaction tray mechanism and the reaction inner tray mechanism shown in FIG. 2 with the heat-insulating cover removed;
FIG. 4 is a perspective view of the reaction disk mechanism shown in FIG. 2 from above and below;
fig. 5 is a perspective view of the reaction disk mechanism shown in fig. 4, as seen from below;
FIG. 6 is a perspective view of the reaction disk mechanism of FIG. 4 at another angle;
FIG. 7 is a perspective view of a reaction carrier plate in the reaction plate mechanism shown in FIG. 6;
FIG. 8 is a schematic cross-sectional structural view of the reaction disk mechanism shown in FIG. 6;
wherein:
1-an incubation device;
11-a buffer tray mechanism;
12-a reaction disk mechanism;
121-reaction carrier plate;
1211-placing holes;
122-reaction disk mounting structure;
1221-a reactive mounting plate;
1222-reaction support column;
1223-reactive mounting holes;
123-reaction disk drive structure;
1231-reaction drive motor;
1232-reaction transmission assembly; 12321-reaction drive wheel; 12322-reaction timing belt;
124-guiding limit structure;
1241-rolling support;
1242-guiding limit slide rail;
1243-lubricating a component;
125-compacting structure;
1251-compressing the mounting base;
1252-hold down guide bar;
1253-compressing the elastic member;
1254-compressing the fixing base;
126-tensioning structure;
1261-tensioning wheel;
1262-tensioning guide bar;
1263-tensioning the elastic member;
1264-tensioning slide rail;
1265-tensioning slide;
1266-tensioning connection means;
127-reaction detection structure;
1271-reaction sensing element;
1272-reaction initializing the test element;
13-a reaction inner disc mechanism;
131-reaction inner tray carrier tray;
14-a cup grabbing mechanism;
2-reaction cup.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the chemiluminescent detector, the incubation device and the reaction plate mechanism of the present invention will be described in further detail below by way of examples with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The terms "coupled" and "connected," as used herein, are intended to encompass both direct and indirect coupling (coupling), unless otherwise indicated. In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element in question must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
Referring to fig. 1 to 3, the present invention provides a reaction disk mechanism 12, where the reaction disk mechanism 12 can perform the operations of adding and mixing the reagent to the reaction cup 2 with the added sample, so that the sample and the reagent are uniformly mixed, and the sample is convenient for the next operation. The reaction disk mechanism 12 is applied to a chemiluminescent detector of the incubation device 1, and the incubation device 1 is used for carrying out incubation operation on samples, reagent adding operation before incubation and the like, so that the chemiluminescent detector can carry out analysis and detection on the incubated samples to obtain corresponding detection results, and the use requirements are met. It should be noted that the specific kind of the sample to be measured is not limited, and in some embodiments, the sample to be measured includes a solid sample or a liquid sample. Further liquid samples include, but are not limited to, blood samples. The reaction disk mechanism 12 can reduce the volume of the executing structure of the reaction cup 2 in the processes of adding reagents and mixing, effectively solves the problems of large occupied space and high cost of the executing structure of the processes of loading the reagents, mixing and the like after the block execution of the incubation reaction device, reduces the occupied space and the cost, and is beneficial to the miniaturization development of instruments.
Referring to fig. 3 to 8, in the present invention, the reaction disk mechanism 12 includes a reaction disk mounting structure 122, a reaction disk driving structure 123, and a reaction carrier disk 121. The reaction disk driving structure 123 is disposed on the reaction disk mounting structure 122, and the reaction carrier disk 121 is mounted on the reaction disk driving structure 123, and the reaction disk driving structure 123 can drive the reaction carrier disk 121 to perform a rotational motion relative to the reaction disk mounting structure 122. Reaction disk mounting structure 122 is used for load-bearing mounting, and other components of reaction disk mechanism 12 are also mounted on reaction disk mounting structure 122. The reaction disk driving structure 123 is mounted on the reaction disk mounting structure 122, the reaction carrier disk 121 is mounted on the reaction disk driving structure 123, and the reaction disk driving structure 123 drives the reaction carrier disk 121 to rotate, so that the reaction carrier disk 121 rotates to a corresponding position, and corresponding operations such as cup adding, reagent adding, mixing, cup taking and the like are performed.
The reaction carrying disk 121 can accommodate the reaction cup 2 with the added sample, and can make the reaction cup 2 complete the reagent adding operation and the mixing operation on the reaction carrying disk 121. The cuvette 2 with the added sample is transferred to the reaction disk mechanism 12, then the reagent adding device of the chemiluminescent detector can transfer the reagent to the cuvette 2 with the added sample on the reaction disk mechanism 12, and then the mixing device of the chemiluminescent detector can mix the cuvette 2 with the added sample and the reagent, so that the sample and the reagent are mixed uniformly. Moreover, the reaction driving mechanism drives the reaction carrying disc 121 to rotate, so that the reaction carrying disc 121 can conveniently move to different positions, and operations such as adding a cup (i.e. loading the reaction cup 2 added with the sample), adding a reagent, mixing and taking a cup (i.e. transferring the reaction cup 2 after mixing away) are respectively executed at corresponding positions, so that the reaction cups 2 at all positions can simultaneously execute corresponding operations, and the operation efficiency of the reaction carrying disc 121 is improved.
In addition, the reaction plate mechanism 12 of the present invention further includes a guiding and limiting structure 124, and the guiding and limiting structure 124 can guide, limit and support the rotational movement of the reaction carrier plate 121. The guide limiting structure 124 can support the reaction bearing plate 121, and meanwhile, when the reaction bearing plate 121 rotates, the guide limiting structure 124 can guide and limit the reaction bearing plate 121, so that the reaction bearing plate 121 rotates stably, the reaction cups 2 on the reaction bearing plate 121 can execute operations at corresponding positions stably, and the reliability of sample processing is guaranteed.
Further, the guiding and limiting structure 124 includes a guiding and limiting sliding rail 1242 and a rolling support 1241, the guiding and limiting sliding rail 1242 is disposed at the bottom of the reaction bearing disc 121, and the guiding and limiting sliding rail 1242 protrudes from the outer peripheral surface of the reaction bearing disc 121. The rolling support 1241 is disposed on the reaction disk mounting structure 122 and located on the peripheral side of the reaction carrier disk 121, and the guiding and limiting rail 1242 is slidably mounted in the rolling support 1241. The rolling support piece 1241 can support the guiding limit sliding rail 1242 arranged in the rolling support piece, so that the problems of falling and tilting of the reaction bearing disc 121 are avoided; when the reaction driving motor 1231 drives the reaction bearing disc 121 to rotate, the reaction bearing disc 121 can drive the guiding limit sliding rail 1242 to synchronously rotate, the guiding limit sliding rail 1242 can roll in the rolling support piece 1241, the rolling support piece 1241 can conduct guiding limit on the guiding limit sliding rail 1242, so that the rotation movement of the reaction bearing disc 121 is stably carried out, the operation reliability of the reaction disc mechanism 12 is improved, and the operation execution of the next procedure is facilitated.
Still further, the rolling support 1241 is a guide limit bearing. Preferably, the periphery of the guiding limit bearing is provided with a sliding groove, and the guiding limit sliding rail 1242 is rollably positioned in the sliding groove. When the reaction bearing disc 121 drives the guiding limit sliding rail 1242 to rotate, the guiding limit sliding rail 1242 can move along the sliding groove, at this time, the inner wall of the sliding groove can support the guiding limit sliding rail 1242 and limit the position of the guiding limit sliding rail 1242, so that the movement track of the guiding limit sliding rail 1242 is fixed, the movement is avoided, the movement track of the reaction bearing disc 121 is fixed, the reaction bearing disc 121 moves stably, and the running stability of the reaction disc mechanism 12 is ensured. Of course, in other embodiments of the present invention, the rolling support 1341 may be other types of support, and the support may be provided with a sliding slot matching with the guiding and limiting rail 1242 to meet the usage requirement.
Optionally, the guiding and limiting structure 124 further includes a lubrication component 1243, where the lubrication component 1243 is disposed on the reaction disc mounting structure 122 and is located on a peripheral side of the reaction bearing disc 121, and the guiding and limiting sliding rail 1242 can contact the lubrication component 1243. The lubrication part 1243 can ensure the lubrication performance between the guiding and limiting sliding rail 1242 and the rolling support part 1241, so as to reduce the friction force generated by sliding of the guiding and limiting sliding rail 1242 in the sliding groove of the rolling support part 1241, ensure the stable movement of the reaction bearing disc 121, ensure the service performance of the guiding and limiting structure 124, and prolong the service life. In the present embodiment, the number of the lubrication members 1243 is one, however, in other embodiments of the present invention, the number of the lubrication members 1243 may be two, three or more, and the plurality of lubrication members 1243 may be uniformly distributed on the outer circumference of the reaction carrier plate 121.
Further, the lubrication part 1243 has a lubrication groove in which the guide limit rail 1242 is located. The lubrication groove is provided with lubricating grease, lubricating oil or other lubricating materials so as to lubricate the guiding limit sliding rail 1242 in the lubrication groove, so that the lubrication performance between the guiding limit sliding rail 1242 and the rolling support part 1241 is improved, and the guiding limit sliding rail 1242 runs stably.
Further, the cross-sectional shape of the portion of the guide limit slide rail 1242 located in the slide groove is adapted to the cross-sectional shape of the slide groove. In this way, the guiding limit sliding rail 1242 is reliably located in the sliding groove of the rolling support member 1241, the rolling support member 1241 and the guiding limit sliding rail 1242 are matched accurately and reliably, and further the supporting, guiding and limiting actions of the rolling support member 1241 on the guiding limit sliding rail 1242 are guaranteed, so that the reaction bearing disc 121 can run stably. Moreover, the cross-sectional shape of the portion of the guide limit rail 1242 located in the lubrication groove is adapted to the cross-sectional shape of the lubrication groove. This ensures the lubrication effect of the lubrication part 1243 on the guide limit slide rail 1242.
Preferably, the cross section of the part of the guiding limit sliding rail 1242 in the sliding groove is V-shaped, the cross section of the sliding groove is V-shaped, and the cross section of the lubrication groove is V-shaped. The mode of adopting the V-shaped guiding limit bearing to be matched with the V-shaped guiding limit sliding rail 1242 is adopted for supporting, guiding and limiting, so that space is saved on space layout, the volume of the reaction disc mechanism 12 is reduced, the volume of the whole machine is reduced, the miniaturized development of an instrument is facilitated, and meanwhile, the cost can be reduced. Of course, in other embodiments of the present invention, the cross-sectional shape of the portion of the guide and limit slide rail 1242 located in the slide groove may be arc-shaped, i-shaped, or otherwise, and the cross-sectional shape of the slide groove is adapted to the cross-sectional shape of the lubrication groove and the cross-sectional shape of the portion of the guide and limit slide rail 1242 located in the slide groove.
The reaction carrier plate 121 is annular, and the guide and limit rail 1242 is annular. Of course, in other embodiments of the present invention, the reaction carrier plate 121 may also have a disc shape, the guiding and limiting rail 1242 has a circular ring shape, and is disposed protruding from the outer peripheral surface of the reaction carrier plate 121. Of course, in other embodiments of the present invention, the guiding and limiting sliding rail 1242 may also be disposed protruding from the inner peripheral surface of the reaction carrier plate 121, and accordingly, the rolling support 1241 is engaged with the guiding and limiting sliding rail 1242 on the inner side of the reaction carrier plate 121.
Still further, the number of rolling supports 1241 is at least three, and at least three of the rolling supports are uniformly distributed around the circumference of the reaction-carrying disk 121. To ensure smooth and reliable rotation of the reaction-carrying disk 121. Of course, in other embodiments of the present invention, other structures than the rolling support 1241 may be used to guide and position the guide and position-limiting slide 1242. In the present embodiment, the number of the rolling supports 1241 is three, and the three rolling supports 1241 are uniformly distributed around the circumference of the reaction-carrying disk 121.
Optionally, the reaction disk mechanism 12 further includes a pressing structure 125, where the pressing structure 125 is disposed on the reaction disk mounting structure 122, and one of the rolling support members 1241 is mounted on the pressing structure 125, and the pressing structure 125 is used for adjusting a distance between the rolling support member 1241 and the guiding limiting sliding rail 1242. Specifically, the pressing structure 125 can drive the rolling support 1241 to move relative to the reaction plate mounting structure 122, so as to adjust the distance between the guiding and limiting slide 1242 and the rolling support 1241. The matching tightness of the guide limit sliding rail 1242 and the rolling support piece 1241 can be adjusted, the rolling support piece 1241 is prevented from being separated from the guide limit sliding rail 1242, so that the gap between the rolling support piece 1241 and the guide limit sliding rail 1242 is reduced, the impact on the rolling support piece 1241 and the guide limit sliding rail 1242 caused by high-speed rotation of the reaction bearing disc 121 is eliminated, the service life is prolonged, and the operation precision of the reaction bearing disc 121 is improved; meanwhile, the reliable support of the rolling support piece 1241 on the guiding limit sliding rail 1242 can be ensured, so that the guiding limit sliding rail 1242 runs stably and reliably, and the reaction bearing disc 121 is ensured to run stably. Of course, in other embodiments of the present invention, each rolling support 1241 may correspond to one compression structure 125.
Further, the pressing structure 125 includes a pressing mounting seat 1251, a pressing elastic member 1253 and a pressing guide rod 1252, the pressing guide rod 1252 is mounted on the reaction disk mounting structure 122, the pressing mounting seat 1251 is slidably disposed on the pressing guide rod 1252, the pressing elastic member 1253 is sleeved on the pressing guide rod 1252, and two ends of the pressing elastic member 1253 are respectively abutted to the pressing guide rod 1252 and the pressing mounting seat 1251. The rolling support member 1241 is mounted on the compression mounting seat 1251, and the compression elastic member 1253 can enable the compression mounting seat 1251 to move on the compression guide rod 1252 and abut against the guide limiting slide rail 1242. It can be appreciated that the compression elastic member 1253 is in a compression state between the compression guide rod 1252 and the compression mounting seat 1251, under the action of the elastic force of the compression elastic member 1253, the compression elastic member 1253 can push the compression mounting seat 1251 to move along the compression guide rod 1252 towards a direction away from the compression elastic member 1253, and then the compression mounting seat 1251 can drive the rolling support member 1241 thereon to move towards the guiding limit sliding rail 1242, so that the rolling support member 1241 is compressed with the guiding limit sliding rail 1242, and the two propping connection is realized. The abutting state of the rolling support piece 1241 and the guiding limit sliding rail 1242 can be always guaranteed, so that the rolling support piece 1241 can always support, guide and limit the guiding limit sliding rail 1242, and stable operation of the reaction bearing disc 121 is guaranteed.
Preferably, one end of the pressing guide rod 1252 has a boss protruding from a surface of the pressing guide rod 1252, the boss being used to limit a position of the pressing elastic member 1253 on the pressing guide rod 1252. The compaction elastic piece 1253 is sleeved on the compaction guide rod 1252, one end of the compaction elastic piece 1253 is abutted against a boss at one end of the compaction guide rod 1252, the other end of the compaction elastic piece 1253 is abutted against the compaction mounting seat 1251, the position movement of the compaction elastic piece 1253 is avoided, and the compaction elastic piece 1253 can be reliably adjusted to compact the mounting seat 1251. Moreover, the pressing elastic member 1253 is a spring, and of course, in other embodiments of the present invention, the pressing elastic member 1253 may be a spring plate or other structures having elastic properties.
Still further, the pressing structure 125 further includes a pressing fixing base 1254, the pressing fixing base 1254 is fixed on the reaction plate mounting structure 122, and the pressing guide rod 1252 is mounted on the pressing fixing base 1254. The pressing fixing seat 1254 plays a role in supporting and fixing, and the pressing guide rod 1252 is fixed on the reaction plate mounting structure 122 through the pressing fixing seat 1254 so as to ensure reliable fixing of the pressing guide rod 1252.
Of course, in other embodiments of the present invention, the following connection modes may be adopted for each part in the compression structure 125, which is specifically described below: the compression fixing seat 1254 is fixedly installed on the reaction plate installation structure 122, the guide limit bearing 1241 is installed on the compression installation seat 1251, and the compression installation seat 1251 is connected with the compression fixing seat 1254 through the compression guide rod 1252. The packing guide rod 1252 passes through the shaft hole of the packing mount 1251 and is inserted into the packing mount 1254. One end of the compression elastic piece 1253 is connected with the compression mounting seat 1251, and the other end of the compression elastic piece 1253 is connected with the compression fixing block 1254. By the elastic action of the compression elastic piece 1253, the guide limit bearing 1241 and the guide limit guide rail 1242 are compressed, and the two are abutted. By adjusting the compression spring 1253, the size of the gap of the abutment between the guide limit bearing 1241 and the guide limit rail 1242 can be adjusted.
As an embodiment, a plurality of placing holes 1211 are opened on the reaction carrier plate 121, the plurality of placing holes 1211 are uniformly distributed along the circumferential direction of the reaction carrier plate 121, and the reaction cups 2 with added samples can be transferred into the plurality of placing holes 1211, respectively. It is understood that the placement hole 1211 may be a through hole penetrating the reaction carrier plate 121 in the axial direction, or may be a blind hole provided in the reaction carrier plate 121 in the axial direction. Further, the plurality of placement holes 1211 are uniformly distributed along the circumferential direction of the reaction-carrying disk 121. This can facilitate the monitoring of the rotational position of the reaction cup 2 on the reaction-carrying disk 121, so that the placement hole 1211 on the reaction-carrying disk 121 is rotated to a corresponding position, facilitating the execution of a corresponding operation. Also, in the present embodiment, a plurality of placement holes 1211 are provided in the circumferential direction of the reaction-carrying disk 121 around one turn.
Further, the reaction tray mounting structure 122 has a plurality of reaction stations including a cup adding station, a reagent adding station, a mixing station, and an incubation cup taking station, which are sequentially arranged. It should be noted that the cup adding station, the reagent adding station, the mixing station, and the incubation cup taking station are disposed around the reaction carrier plate 121 and are fixed on the reaction plate mounting structure 122, and the cup adding station, the reagent adding station, the mixing station, and the incubation cup taking station do not change with rotation of the reaction carrier plate 121. The reaction disk driving structure 123 can drive the reaction carrier disk 121 to rotate the placement holes 1211 thereon to the corresponding reaction stations, and perform the corresponding operations at the reaction stations.
The cup adding station is provided corresponding to the cup grabbing mechanism 14, and the cup grabbing mechanism 14 can place the reaction cup 2 with the added sample into the placing hole 1211 of the reaction carrying disk 121 at the cup adding station. The reagent adding station is arranged corresponding to a reagent adding device, and the reagent adding device can add reagent into the reaction cup 2 of the reaction bearing disc 121 in the reagent adding station. The mixing station is provided corresponding to a mixing device, and the mixing device can mix the sample in the reaction cup 2 of the reaction carrying disc 121 with the reagent at the mixing station. The incubation cup-taking station is arranged corresponding to the cup-grabbing mechanism 14, and the cup-grabbing mechanism 14 can grab the reaction cup 2 in the reaction carrying tray 121 at the incubation cup-taking station and transfer the reaction cup 2 into a structure for incubation.
Specifically, the reaction disk driving structure 123 drives the reaction carrier disk 121 to drive the placement hole 1211 to move to the cup adding station, and the cup grabbing mechanism 14 can transfer the reaction cup 2 with the added sample into the placement hole 1211 corresponding to the cup adding station. The reaction disk driving structure 123 drives the reaction bearing disk 121 to drive the reaction cup 2 in the placement hole 1211 to rotate to a reagent adding station, and the reagent adding device adds reagent into the reaction cup 2 in the corresponding placement hole 1211 at the reagent adding station; the reaction disk driving structure 123 drives the reaction carrying disk 121 to drive the reaction cup 2 in the placement hole 1211 to rotate to the mixing station, and the mixing device uniformly mixes the sample in the reaction cup 2 corresponding to the placement hole 1211 with the reagent at the mixing station. The reaction disk driving structure 123 drives the reaction bearing disk 121 to drive the reaction cups 2 in the placement holes 1211 to move to the incubation cup taking stations, and the cup grabbing mechanism 14 transfers the reaction cups 2 in the placement holes 1211 corresponding to the incubation cup taking stations to the reaction inner disk mechanism 13.
It will be appreciated that the reaction disk driving structure 123 drives the reaction carrier disk 121 to drive the reaction cup 2 thereon to rotate at the corresponding station, so that the reaction cup 2 is subjected to the corresponding operations, such as cup adding, reagent adding, mixing and cup taking operations, at the corresponding station. Also, the movement trace of one placement hole 1211 in the reaction-carrying disk 121 is: the cup grasping mechanism 14 places the cuvette 2 with the sample added into the placement hole 1211 of the reaction carrying tray 121 at the cup adding station; subsequently, the reaction disk drive structure 123 drives the reaction carrier disk 121 to rotate so that the reaction cups 2 of the placement holes 1211 move from the cup adding station to the reagent adding station where the reagent adding device adds reagent into the reaction cups 2 of the placement holes 1211; after reagent addition is completed, the reaction disk driving structure 123 drives the reaction bearing disk 121 to rotate, so that the reaction cup 2 of the placement hole 1211 moves from the reagent adding station to the mixing station, and the mixing device uniformly mixes the sample in the reaction cup 2 of the placement hole 1211 with the reagent at the mixing station; after the mixing is completed, the reaction disk driving structure 123 drives the reaction carrying disk 121 to rotate, so that the reaction cup 2 of the placing hole 1211 moves from the mixing station to the incubation cup taking station, and the cup grabbing mechanism 14 takes out the reaction cup 2 in the placing hole 1211 at the incubation cup taking station and transfers the reaction cup 2 to the reaction inner disk mechanism 13; subsequently, the reaction disk driving structure 123 drives the reaction carrier disk 121 to rotate, so that the reaction carrier disk 121 drives the placement holes 1211 to return to the cup adding station, and the reaction carrier disk 121 is operated continuously in a circulating manner.
It will be appreciated that the movement of the placement hole 1211 in the reaction carrier plate 121 corresponds to the operations of adding a cup, adding a reagent, mixing, and taking a cup, and corresponds to the cup adding station, the reagent adding station, the mixing station, and the incubation cup taking station, respectively. Also, the movement paths of the plurality of placement holes 1211 are all the same, i.e., the plurality of placement holes 1211 can perform the above-described operations, respectively. In addition, the plurality of placing holes 1211 are respectively corresponding to the plurality of reaction stations, so that the operation of each reaction station of the reaction disc mechanism 12 can be linked, all the placing holes 1211 or part of the placing holes 1211 can respectively correspond to each reaction station, namely, the reaction disc driving structure 123 drives the reaction bearing disc 121 to drive the placing holes 1211 at the incubation cup taking station to rotate to the cup adding station, meanwhile, the corresponding reaction cup 2 at the cup adding station is carried to the reagent adding station by the reaction bearing disc 121, the reaction cup 2 at the reagent adding station is driven to the mixing station by the reaction bearing disc 121, and the reaction cup 2 at the mixing station is driven to the incubation cup taking station by the reaction bearing disc 121; subsequently, the cuvette grabbing mechanism 14 transfers the cuvette 2 with the added sample to the placing hole 1211 of the corresponding cuvette adding station of the reaction carrying disc 121, meanwhile, the reagent adding device adds reagent to the corresponding cuvette 2 at the reagent adding station, the mixing device mixes the sample and the reagent in the cuvette 2 at the corresponding position, and the cuvette grabbing mechanism 14 grabs the cuvette 2 at the corresponding position and transfers the cuvette 2 to the reaction inner disc mechanism 13; after each action is completed, the reaction disk driving structure 123 drives the reaction carrier disk 121 to rotate again, so that each placement hole 1211 rotates to the next process step respectively, the reaction cup 2 corresponds to each reaction station, and the next round of action is performed. Through the sequential setting and the linkage setting of each reaction station for each reaction station can operate simultaneously, can improve the treatment effeciency of sample, and then shorten the time that sample incubation process consumed, improve the detection efficiency of whole chemiluminescence detector.
It should be noted that, the cup grabbing mechanism 14 may include one or more cup grabbing structures, where the plurality of cup grabbing structures are respectively configured to perform different operations, such as placing the reaction cup 2 on the reaction carrier plate 121 or taking the reaction cup 2 out of the reaction carrier plate 121, so that each operation can be performed simultaneously, and the transfer efficiency of the reaction cup 2 is improved, and further the operation efficiency of the whole machine is improved. Moreover, the cup grabbing structure can adopt a cup grabbing driving assembly, a cup grabbing control assembly, a cup grabbing arm assembly and the like to grab and transfer the reaction cup 2. It will be appreciated that the gripper control assembly may employ a general control system such as a controller or the like, and the gripper drive assembly may employ a drive motor in combination with a gear drive assembly, belt drive assembly, chain drive assembly or the like to effect movement control of the gripper arm assembly to move the gripper arm assembly into position and grip the transfer cuvette 2.
Optionally, the reaction station further comprises a cleaning and cup placing station, and the cleaning and cup placing station is positioned between the cup adding station and the reagent adding station. The sample after the incubation of the reaction inner tray mechanism 13 is transferred to the washing device by the cuvette grabbing mechanism 14 for washing operation, so that impurities which cannot be fully mixed with the sample in the cuvette 2 are washed away, and since part of the sample also needs to be added with a reagent for reaction, at this time, the cuvette grabbing mechanism 14 can transfer the cuvette 2 after the washing in the washing device to the placing hole 1211 of the reaction carrying tray 121. It will be appreciated that the cleaning and cup placing station is located before the reagent adding station, so that when the reaction disc driving structure 123 drives the reaction carrying disc 121 to rotate sequentially, the reaction cups 2 on the cleaning and cup placing station can be transferred to the reagent adding station for secondary reagent adding operation, after reagent is added, the reaction cups move to the incubation cup taking station, and are transferred to the reaction inner disc mechanism 13 by the cup grabbing mechanism 14 for incubation operation. Also, when the cleaning and putting station needs to put the cleaned cuvette 2, one putting hole 1211 of the reaction carrying plate 121 needs to be reserved. It will be appreciated that the wash and place station may be located at any point prior to the reagent addition station so that the reagent addition device can add reagent at the reagent addition station to the washed reaction cup 2 moved to that station. In this embodiment, the cleaning and cup placing station is located behind the cup adding station, because the cleaning and cup placing station is located closer to the cleaning device, so that the reaction cup 2 can be conveniently transferred. .
Optionally, the reagent adding station comprises a first reagent adding station and a second reagent adding station, and the first reagent adding station and the second reagent adding station are positioned between the cleaning cup placing station and the mixing station. Because different samples need to be added with different reagents during the reaction, two reagent adding stations are arranged to meet the use requirements of different samples. And each sample can be selected to carry out reagent adding operation at the first reagent adding station, the second reagent adding station or the first reagent adding station and the second reagent adding station according to actual use requirements. The reagent adding device of the chemiluminescent detector transfers the reagent through the reagent arm, and the reagent arm can suck the reagent at the reagent sucking position and rotate to the reagent adding station to add the reagent into the reaction cup 2. However, because the reagent arm needs a certain time to rotate between the reagent sucking position and the reagent adding station, namely, the time required for the reagent arm to rotate to the reagent sucking position to suck the reagent and then to rotate back to the reagent adding station to discharge the reagent is respectively longer than the cup adding operation time, the mixing operation time and the cup taking operation time, the two reagent adding stations are arranged to ensure that the reagent arm has enough time to finish the reagent sucking and reagent adding operations, so that the reaction bearing disc 121 does not need to wait until the reagent cup 2 is completely added and then rotates. This can shorten the operation time of the reaction carrying disk 121 to improve the operation efficiency of the reaction carrying disk 121 and further improve the operation speed of the whole machine.
Optionally, the reaction disk mounting structure 122 further has a buffer station, where the buffer station is located between any two adjacent reaction stations, and the buffer station can make the distances between the two adjacent reaction stations and the buffer station equal. It will be appreciated that the buffer station is a waiting station, and the reaction carrier tray 121 moves the placement hole 1211 thereon to the buffer station without performing any operation, so that an operation time can be provided for the placement hole 1211 to perform the corresponding operation.
Preferably, the buffer station comprises a first buffer station, a second buffer station and a third buffer station, and the cup adding station, the first buffer station, the cleaning and cup placing station, the second buffer station, the first reagent adding station, the second reagent adding station, the mixing station, the incubation cup taking station and the third buffer station are arranged at equal intervals along the circumferential direction of the reaction bearing disc 121 in sequence. The reaction disk driving structure 123 drives the reaction carrier disk 121 to drive the placement holes 1211 thereon to move to the reaction station and the buffer station, respectively, and performs the corresponding operations.
Further, the number of the placement holes 1211 on the reaction carrier plate 121 is an integer multiple of the sum of the number of the reaction stations and the buffer stations. That is, when the reaction disk driving structure 123 drives the reaction carrier disk 121 to rotate, all the reaction stations and the buffer stations on the reaction disk mounting structure 122 can respectively correspond to at least part of the placement holes 1211 on the reaction carrier disk 121. Preferably, the number of the placement holes 1211 on the reaction carrier plate 121 is equal to the sum of the number of the reaction stations and the buffer stations, i.e., nine.
In this embodiment, nine stations on the reaction disk mounting structure 122 are a cup adding station, a first buffer station, a cleaning and cup placing station, a second buffer station, a first reagent adding station, a second reagent adding station, a mixing station, an incubation cup taking station, and a third buffer station, which are respectively and uniformly distributed around the circumferential direction of the reaction carrier disk 121 in the clockwise direction, and when the reaction carrier disk 121 is driven to rotate clockwise by the reaction disk driving structure 123, each placement hole 1211 on the reaction carrier disk 121 can respectively correspond to the cup adding station, the first buffer station, the cleaning and cup placing station, the second buffer station, the first reagent adding station, the second reagent adding station, the mixing station, the incubation cup taking station, and the third buffer station, and execute corresponding operations at corresponding positions. Of course, in other embodiments of the present invention, the cup adding station, the first buffer station, the cleaning and cup placing station, the second buffer station, the first reagent adding station, the second reagent adding station, the mixing station, the incubation and cup taking station, and the third buffer station may be arranged in a counter-clockwise order, where the reaction disk driving structure 123 drives the reaction carrier device to rotate counter-clockwise to correspond to each station.
It should be noted that, each time the reaction disk driving structure 123 drives the reaction carrier disk 121 to rotate, the reaction carrier disk 121 rotates about its center by 1/9 of a circle, so that nine placement holes 1211 on the reaction carrier disk 121 can be respectively configured corresponding to nine stations, and corresponding operations are performed by devices corresponding to the stations. After the above arrangement and the corresponding operation structure are matched with each station, the working efficiency of the reaction bearing disc 121 can be effectively improved, so that the processing flow between the reaction bearing disc 121 and other functional modules such as the buffer disc mechanism 11, the reagent adding device and the reaction inner disc mechanism 13 is smoother, and the operation efficiency of the chemiluminescent detector is improved.
As an embodiment, the reaction disk driving structure 123 includes a reaction driving motor 1231 and a reaction transmission assembly 1232, the reaction driving motor 1231 is installed on the reaction disk mounting structure 122, the reaction transmission assembly 1232 is in transmission connection with the output end of the reaction driving motor 1231 and the reaction carrying disk 121, and the reaction driving motor 1231 drives the reaction transmission assembly 1232 to drive the reaction carrying disk 121 to rotate relative to the reaction disk mounting structure 122. Also, the reaction disk mounting structure 122 includes a reaction mounting base plate 1221 and reaction support columns 1222 for supporting the reaction mounting base plate 1221. The reaction driving motor 1231 is installed on the reaction installation base 1221, and the pressing structure 125 is installed on the reaction installation base 1221. The reaction support columns 1222 can support the reaction mounting base 1221 on the top of a chemiluminescent detector. Preferably, in the present embodiment, the reaction tray mounting structure 122 is mounted on the reaction tray mechanism 13 of the incubation apparatus 1, and the reaction mounting base 1221 is supported on the reaction tray mechanism 13 by the reaction support columns 1222. The reaction mounting plate 1221 functions as a load bearing support, and the reaction disk drive structure 123 is mounted on the reaction mounting plate 1221. Optionally, a reaction mounting hole 1223 is formed on the reaction mounting base plate 1221, and a reaction support column 1222 is mounted in the reaction mounting hole 1223, so that the reaction support column 1222 supports the reaction mounting base plate 1221 on the reaction inner disk mechanism 13.
The reaction disk drive structure 123 includes a reaction drive motor 1231 and a reaction transmission assembly 1232. The reaction transmission assembly 1232 is in transmission connection with the output end of the reaction driving motor 1231 and the reaction carrying disk 121. The reaction driving motor 1231 drives the reaction driving assembly 1232 to rotate the reaction carrying tray 121, so that each of the placing holes 1211 on the reaction carrying tray 121 moves to each of the stations, and performs a corresponding operation. Preferably, the reaction transmission assembly 1232 includes a reaction driving wheel 12321 and a reaction synchronous belt 12322, the reaction driving wheel 12321 is mounted on the output end of the reaction driving motor 1231, and the reaction synchronous belt 12322 is sleeved on the reaction driving wheel 12321 and the reaction bearing disc 121. The reaction driving motor 1231 drives the reaction driving wheel 12321 to rotate, and the reaction driving wheel 12321 drives the reaction bearing disc 121 to rotate through the reaction synchronous belt 12322, so that the reaction bearing disc 121 performs circular motion, and the reaction bearing disc is used for realizing that the placing holes 1211 move to a cup adding station, a reagent adding station, a mixing station, an incubation cup taking station and the like to perform corresponding operations. Moreover, the outer wall of the reaction bearing disc 121 is provided with teeth, and the teeth are matched with the reaction synchronous belt 12322, so that the reaction synchronous belt 12322 is prevented from slipping, and the transmission reliability is ensured. Of course, in other embodiments of the present invention, the reaction transmission assembly 1232 may also be a gear transmission structure, a sprocket transmission structure, or other transmission structure capable of achieving rotation of the reaction carrier plate 121.
Optionally, the reaction plate mechanism 12 further includes a tensioning structure 126, the tensioning structure 126 is disposed on the reaction plate mounting structure 122, and the tensioning structure 126 abuts against the reaction plate timing belt 12322. The tensioning structure 126 can be always abutted against the reaction synchronous belt 12322, so that the reaction synchronous belt 12322 is always in a tensioning state, and reliable transmission of the reaction synchronous belt 12322 is ensured.
Referring to fig. 4, in an embodiment of the present invention, the tensioning structure 126 includes a tensioning wheel 1261, a tensioning wheel shaft, a tensioning guide rod 1262 and a tensioning elastic member 1263, the tensioning guide rod 1262 is fixed on the reaction plate mounting structure 122, the tensioning wheel 1261 is rotatably disposed on the tensioning wheel shaft, the tensioning wheel 1261 is located at the outer side of the reaction timing belt 12322, one end of the tensioning elastic member 1263 is fixed on the tensioning guide rod 1262, the other end of the tensioning elastic member 1263 is connected with the tensioning wheel shaft, and the tensioning elastic member 1263 can move along the tensioning guide rod 1262, so that the tensioning wheel 1261 abuts against the reaction timing belt 12322. The tensioning wheel 1261 can be abutted with the reaction synchronous belt 12322, when the reaction synchronous belt 12322 rotates, the reaction synchronous belt 12322 can drive the tensioning wheel 1261 to rotate relative to the tensioning wheel shaft, so that interference between the tensioning wheel 1261 and the reaction synchronous belt 12322 can be avoided, and reliable transmission of the reaction synchronous belt 12322 is ensured. It can be appreciated that the tensioning elastic member 1263 is in a compressed state, when the reaction timing belt 12322 is loosened, the tensioning elastic member 1263 can extend along the tensioning guide rod 1262, at this time, the tensioning elastic member 1263 can drive the tensioning wheel 1261 to move, so that the tensioning wheel 1261 is always abutted against the reaction timing belt 12322, and further the reaction timing belt 12322 is ensured to be always kept in a tensioned state. Preferably, the tensioning elastic member 1263 is a spring, and the spring is sleeved on the tensioning guide rod 1262. Of course, in other embodiments of the present invention, the spring or other structure having elastic properties.
Further, the tensioning structure 126 further includes a tensioning slide rail 1264 and a tensioning slider 1265, the tensioning slide rail 1264 is disposed on the reaction plate mounting structure 122, the tensioning wheel shaft is fixed on the tensioning slider 1265, the tensioning slider 1265 is slidably disposed on the tensioning slide rail 1264, the tensioning elastic member 1263 is connected with the tensioning slider 1265, and the tensioning elastic member 1263 can drive the tensioning slider 1265 to move along the tensioning slide rail 1264 and drive the tensioning wheel shaft and the tensioning wheel 1261 thereon to move. When the tensioning elastic piece 1263 stretches along the tensioning guide rod 1262, the tensioning elastic piece 1263 drives the tensioning sliding block 1265 to move, and the tensioning sliding block 1265 can drive the tensioning wheel shaft and the tensioning wheel 1261 on the tensioning elastic piece to synchronously move, so that the tensioning wheel 1261 can tension the reaction synchronous belt 12322; at the same time, the movement of tensioning slide 1265 along tensioning slide 1264 can ensure a movement path of tensioning slide 1265 such that tensioning slide 1265 can move in the direction of tensioning reaction timing belt 12322. Preferably, the tension slide 1264 extends toward the inside of the reaction timing belt 12322.
Still further, the tensioning structure 126 further includes a tensioning connection member 1266, the tensioning connection member 1266 is connected to the tensioning slider 1265 and the tensioning elastic member 1263, and the tensioning elastic member 1263 drives the tensioning slider 1265 to move through the tensioning connection member 1266. Preferably, the tension connecting member 1266 is L-shaped, one end of the L-shaped tension connecting member 1266 is connected to the tension slider 1265, and the other end of the L-shaped tension connecting member 1266 is connected to the tension elastic member 1263.
It will be appreciated that when the reaction drive assembly 1232 is not driven by the reaction timing belt 12322, tensioning of the reaction drive assembly 1232 is not required. In this embodiment, the tensioning wheel 1261 is always in contact with the reaction timing belt 12322 when the reaction timing belt 12322 is in operation; in the transmission process, if the reaction synchronous belt 12322 has a loosening phenomenon, the tensioning elastic piece 1263 can stretch along the tensioning guide rod 1262, the tensioning elastic piece 1263 drives the tensioning connecting part 1266 to move, then the tensioning connecting part 1266 drives the tensioning sliding block 1265 to slide along the tensioning sliding rail 1264, at this time, the tensioning sliding block 1265 can tension the reaction synchronous belt 12322 through the tensioning wheel 1261 thereon, so that the reaction synchronous belt 12322 is always in a tensioning state, and the reliable operation of the reaction synchronous belt 12322 is ensured.
In another embodiment of the present invention, the reaction plate mounting structure 122 further includes a motor mounting plate, and the tensioning structure 126 is connected to the reaction plate mounting base plate 1221 and the motor mounting plate, and the reaction plate timing belt 12322 is tensioned by the tensioning structure 126 to ensure reliable transmission. Specifically, the reaction driving motor 1231 is fixed on the motor mounting plate through a screw, the motor mounting plate is adjustably disposed on the reaction plate mounting base plate 1221, the tensioning structure 126 includes an adjusting component, the adjusting component penetrates through the motor mounting plate and abuts against the reaction plate mounting base plate 1221, and the adjusting component can adjust the distance between the reaction plate mounting base plate 1221 and the motor mounting plate to adjust the tensioning degree of the reaction plate synchronous belt 12322. Specifically, the adjusting component is an adjusting screw, and the adjusting screw penetrates through the motor mounting plate and is abutted to the reaction plate mounting base plate 1221, and the adjusting screw is screwed down, so that the motor mounting plate moves towards a direction away from the reaction plate mounting base plate 1221, the distance between the reaction plate bearing plate 121 and the reaction plate driving wheel 12321 can be increased, and the tensioning degree of the reaction plate synchronous belt 12322 is adjusted.
Further, the tensioning device 126 further includes a fixing component, the motor mounting plate is provided with an adjusting hole, the adjusting hole extends along a direction that the motor mounting plate is far away from the reaction plate mounting base 1221, and the fixing component penetrates through the adjusting hole to be mounted on the reaction plate mounting base 1221. When the adjusting part adjusts the distance between the reaction plate mounting base 1221 and the motor mounting plate, the motor mounting plate can move through the cooperation of the fixing part and the adjusting hole, and after the adjusting part adjusts, the fixing part can fix the motor mounting plate on the reaction plate mounting base 1221, so that the motor mounting plate is reliably fixed on the reaction plate mounting base 1221. Preferably, the fixing member is a fixing screw.
The tensioning process of the reaction disc synchronous belt 12322 by adopting the adjusting part and the fixing part is as follows: when the motor is installed, the distance between the reaction plate installation base 1221 and the motor installation plate is adjusted by the adjusting component, and after the distance is adjusted to a proper distance, the motor installation plate is fixed on the reaction plate installation base 1221 by the fixing component. The adjusting principle of the adjusting part is as follows: the screw end of the adjusting screw is in threaded connection with the motor mounting plate and passes through the motor mounting plate to be abutted with the reaction plate mounting base plate 1221, and in the process of screwing the adjusting screw, as the end of the adjusting screw is abutted on the reaction plate mounting base plate 1221, the motor mounting plate can move towards the direction away from the reaction plate mounting base plate 1221, so that the aim of tensioning the reaction plate synchronous belt 12322 is fulfilled.
It will be appreciated that when the reaction plate transmission assembly 1232 does not employ the reaction plate timing belt 12322 for transmission, the reaction plate transmission assembly 1232 may not need to be tensioned, and at this time, the reaction plate mounting base 1221 may be integrally structured with the motor mounting plate, that is, the reaction plate driving motor 1231 may be directly mounted on the reaction plate mounting base 1221. When the tensioning structure 126 adopts the structure in one embodiment to tension the reaction disc timing belt 12322, the reaction disc mounting base 1221 may be integrally constructed with the motor mounting plate, and the reaction disc driving motor 1231 may be directly mounted on the reaction disc mounting base 1221; the reaction plate mounting base 1221 and the motor mounting plate may be separately provided, and the motor mounting plate is fixed to the reaction plate mounting base 1221 by screw members.
Optionally, the reaction plate mechanism 12 further includes a reaction detecting structure 127, the reaction detecting structure 127 is disposed on the reaction mounting base plate 1221, and the reaction detecting structure 127 is used for initializing the reaction carrying plate 121. The reaction detecting structure 127 includes a reaction sensing element 1271 and a reaction initializing detecting element 1272, the reaction sensing element 1271 is disposed on the reaction carrying tray 121, and the reaction initializing detecting element 1272 is mounted on the reaction mounting base 1221. The reaction initiation detecting member 1272 can cooperate with the reaction sensing member 1271 to detect the initial position of the reaction carrier plate 121 and initialize the reaction carrier plate 121. It can be understood that the initial positions of the reaction initiation detecting member 1272 and the reaction sensing member 1271 for detecting the reaction carrying tray 121 are realized by controlling the reaction driving motor 1231, so that the reaction driving motor 1231 can be at the initial position, and the position of the reaction carrying tray 121 can be accurately monitored by the number of steps of the movement of the reaction driving motor 1231, so that the movement of the reaction carrying tray 121 is accurate and reliable. In this embodiment, the reaction sensing element 1271 is an optocoupler sensing piece, and the reaction initialization detecting element 1272 may be a detection optocoupler; of course, in other embodiments of the present invention, the reaction sensing element 1271 and the reaction initiation detecting element 1272 may be hall switches or other components capable of initiating detection.
Referring to fig. 1 to 3, the present invention further provides an incubation device 1, where the incubation device 1 can carry an empty reaction cup 2, samples and reagents are added into the reaction cup 2 at the incubation device 1, and the adding, mixing and incubation actions of the samples and the reagents are performed at different positions, so that the operations of the samples and the reagents do not interfere with each other, and each operation can be performed simultaneously, so that the problems of long operation time caused by time occupation and large space occupation when each process of the current sample reaction is performed are effectively solved, the operation efficiency of the incubation device 1 is ensured, the detection speed of a chemiluminescent detector is increased, and the detection efficiency is further ensured.
In the present invention, the incubation apparatus 1 includes a buffer tray mechanism 11, a reaction inner tray mechanism 13, and a reaction tray mechanism 12 in any of the above embodiments. The buffer tray mechanism 11 can carry empty cuvettes 2, and the empty cuvettes 2 can perform a sample addition operation on the buffer tray mechanism 11. Specifically, an empty cuvette 2 is transferred to the buffer tray mechanism 11, and then the sample loading device of the chemiluminescent detector is able to transfer the sample into the cuvette 2 above the buffer tray mechanism 11. The reaction disk mechanism 12 can carry the reaction cup 2 with the added sample, and the reaction cup 2 with the added sample performs the reagent adding operation and the mixing operation on the reaction disk mechanism 12. Specifically, the cuvette 2 with the sample added on the buffer tray mechanism 11 is transferred to the reaction tray mechanism 12, then, the reagent adding device of the chemiluminescent detector can transfer the reagent to the cuvette 2 with the sample added on the reaction tray mechanism 12, and then, the mixing device of the chemiluminescent detector can mix the cuvette 2 with the sample and the reagent, so that the sample and the reagent are mixed uniformly. The reaction inner tray mechanism 13 is capable of carrying the cuvette 2 after mixing and incubating the sample in the cuvette 2 with the reagent. Specifically, the reaction cup 2 in the reaction disk mechanism 12 can be transferred to the reaction inner disk mechanism 13, and then, the reaction inner disk mechanism 13 can incubate the uniformly mixed sample and the reagent in the reaction cup 2, so that the sample reaches the optimal reaction condition, and the sample parameters can be conveniently detected by the luminescence detection device of the chemiluminescent detector.
The buffer tray mechanism 11 comprises a rotatable buffer carrier tray, and the buffer carrier tray can accommodate the cuvette 2 and enable the cuvette 2 to finish the sample adding operation on the buffer carrier tray. The reaction carrying tray 121 of the reaction tray mechanism 12 can accommodate the cuvette 2 transferred from the buffer tray mechanism 11, and can make the cuvette 2 perform reagent adding operation and mixing operation on the reaction tray mechanism 12. The reaction disk mechanism 13 includes a rotatable reaction disk carrier 131, and the reaction disk carrier 131 is capable of accommodating the cuvette 2 transferred from the reaction carrier disk 121 and of performing an incubation operation on the reaction disk mechanism 13. The reaction carrier plate 121 and the reaction inner plate carrier plate 131 can be rotated independently.
The empty reaction cup 2 is transferred to the buffer bearing disc, and then the sample adding device adds samples to the reaction cup 2 above the buffer bearing disc; after the addition of the sample is completed, the reaction cup 2 with the sample is transferred to the reaction carrying disc 121, the reagent adding device adds a reagent into the reaction cup 2 with the sample in the reaction carrying disc 121, and after the reagent addition is completed, the mixing device carries out mixing operation on the reaction cup 2 in the reaction carrying disc 121, so that the sample and the reagent are mixed uniformly; after the completion of the mixing, the cuvette 2 after the sample and the reagent are mixed uniformly is transferred to the reaction inner tray 131, and an incubation operation is performed in the reaction inner tray 131, and after the incubation operation is completed, the cuvette 2 in the reaction inner tray 131 is transferred away for the next operation. It will be appreciated that the empty cuvette 2 is transferred from the cuvette 2 automatic transfer device of the chemiluminescent detector to the buffer carrier plate, however, in other embodiments of the present invention, the empty cuvette 2 may be loaded from outside the chemiluminescent detector, so long as it is ensured that the empty cuvette 2 is transported to the buffer carrier plate to meet the requirements. Preferably, the buffer carrier plate is disc-shaped, however, in other embodiments of the present invention, the buffer carrier plate may be oval, quadrilateral, or other shapes capable of carrying the reaction cup 2.
It should be noted that, the rotatable setting of buffering loading tray can make things convenient for empty reaction cup 2 to shift to reaction loading tray 121 for the buffering loading tray needs to place the cup position that adds of reaction cup 2 and is close to the automatic transmission device department of reaction cup 2 of chemiluminescent detector, can reduce the reaction cup 2 like this from the automatic transmission device of reaction cup 2 to the motion distance of buffering loading tray, improves the transfer rate of reaction cup 2. In addition, the buffer bearing disc can also drive the reaction cup 2 to be added with the sample to move towards the sample adding position of the sample adding device, so that the efficiency of sample adding of the sample adding device into the reaction cup 2 of the buffer bearing disc can be improved; in addition, the reaction cup 2 with the added sample in the buffer bearing plate can also move towards the direction approaching to the reaction bearing plate 121, so that the moving distance of the reaction cup 2 with the added sample from the buffer bearing plate to the reaction bearing plate 121 can be reduced, and the transfer speed of the reaction cup 2 can be improved. The reaction bearing disc 121 can be rotatably arranged to facilitate the reaction bearing disc 121 to move to different positions, and operations such as adding a cup (namely, loading the reaction cup 2 with the added sample), adding a reagent, mixing, taking a cup (namely, transferring the reaction cup 2 after mixing away) and the like are respectively executed at corresponding positions, so that the reaction cups 2 at all positions can simultaneously execute corresponding operations, and the operation efficiency of the reaction bearing disc 121 is improved. The rotatable arrangement of the reaction inner tray 131 can enable the position of the reaction inner tray 131 where the reaction cup 2 needs to be placed to be close to the cup taking position of the reaction tray 121, so that the transfer path of the reaction cup 2 is reduced. Through the cooperation of rotatable buffering loading tray, rotatable reaction loading tray 121 and rotatable reaction inner tray loading tray 131, can improve the motion efficiency of instrument, and then guarantee detection efficiency.
The buffer carrier, the reaction carrier 121, and the reaction inner tray 131 are rotatable independently of each other. It will be appreciated that the movements of the buffer carrier disc, the reaction carrier disc 121 and the reaction inner disc carrier disc 131 do not interfere with each other, and the buffer carrier disc does not affect the cup filling and reagent filling operation of the reaction carrier disc 121 and the incubation cup filling and taking operation of the reaction inner disc carrier disc 131 when the buffer carrier disc performs the cup filling and sample filling operation. Namely, the buffer bearing disc needs to be in a static state when the cup is empty and the sample is added, at this time, the reaction bearing disc 121 can be in a rotating state, and the reaction inner disc bearing disc 131 can also be in a rotating state; the reaction carrying disc 121 needs to be in a static state when performing the operations of adding a cup, adding a reagent, mixing uniformly and taking a cup, at this time, the buffer carrying disc can be in a rotating state, and the reaction inner disc carrying disc 131 can also be in a rotating state; the reaction inner tray 131 needs to be in a static state when adding and taking cups, and at this time, the buffer tray may be in a rotating state, and the reaction tray 121 may also be in a rotating state. In this way, the respective functions of the incubation apparatus 1 are allocated to the buffer carrier tray, the reaction carrier tray 121, and the reaction inner tray carrier tray 131, and the buffer carrier tray, the reaction carrier tray 121, and the reaction inner tray carrier tray 131 can perform the corresponding actions, respectively, without waiting for the previous operation, so that the operations on the buffer carrier tray, the reaction carrier tray 121, and the reaction inner tray carrier tray 131 are performed simultaneously, the speed of incubating the sample by the incubation apparatus 1 is increased, and the running time of the incubation apparatus 1 at the time of incubating the sample is shortened.
In operation of the incubation apparatus 1 of the present invention, empty cuvette 2 is transferred to the buffer carrier tray of the buffer tray mechanism 11, and a sample is added to the empty cuvette 2, and then the cuvette 2 to which the sample is added is transferred from the buffer carrier tray to the reaction carrier tray 121 of the reaction tray mechanism 12, and a reagent is added to the cuvette 2 of the reaction carrier tray 121, and a mixing operation is performed; the reaction cup 2 after mixing is transferred from the reaction bearing disc 121 to the reaction inner disc bearing disc 131 of the reaction inner disc mechanism 13, the operation of incubation of the reaction cup 2 after mixing is completed on the reaction inner disc bearing disc 131, and as the buffer bearing disc, the reaction bearing disc 121 and the reaction inner disc bearing disc 131 rotate independently, the buffer bearing disc 121 can perform operations such as cup adding, reagent adding, mixing and cup taking when performing operations of cup adding and sample adding, the reaction inner disc bearing disc 131 can perform operations of cup adding and cup taking, operations of the reaction inner disc bearing disc 131 can be performed simultaneously without interference, the problem of long operation time caused by occupation time and occupation of large space when each section of procedure of the current sample reaction is performed is effectively solved, the detection speed of the chemiluminescent detector is improved, and the detection efficiency is further ensured.
As an embodiment, the reaction carrier plate 121 has a circular ring shape, and the reaction inner plate carrier plate 131 has a circular disk shape; the reaction carrying disc 121 is sleeved outside the reaction inner disc carrying disc 131, and the buffer carrying disc is independent of the reaction carrying disc 121. The reaction inner tray 131 is positioned at the inner side of the reaction tray 121, so that the distance of transferring the reaction cups 2 in the reaction tray 121 to the reaction inner tray 131 can be reduced; at the same time, the space occupied by the reaction carrying disk 121 and the reaction inner disk carrying disk 131 can be reduced, so that the volume of the chemiluminescent detector can be reduced. Moreover, the buffer carrier plate is independent of the reaction carrier plate 121 and the reaction inner plate carrier plate 131, so that the sample adding operation and the reagent adding and incubation operation of the sample are separated, the buffer plate mechanism 11, the reaction plate mechanism 12 and the reaction inner plate mechanism 13 are conveniently arranged, and meanwhile, the movement of the buffer carrier plate, the reaction carrier plate 121 and the reaction inner plate carrier plate 131131 and the movement of devices, such as a sample adding device, a reagent adding device and the like, matched with the buffer carrier plate, the reaction carrier plate 121 and the reaction inner plate carrier plate 131 for executing corresponding operations can not be interfered.
In still another embodiment of the present invention, the reaction carrier plate 121 is sleeved outside the reaction inner plate carrier plate 131, and the buffer carrier plate is sleeved outside the reaction carrier plate 121. It can be understood that the buffer bearing disc, the reaction bearing disc 121 and the reaction inner disc bearing disc 131 are arranged in a layer-by-layer sleeved mode, the buffer bearing disc is located at the outermost side, the reaction inner disc bearing disc 131 is located at the innermost side, and the reaction cup 2 is transferred onto the buffer bearing disc, then onto the reaction bearing disc 121 and then onto the reaction inner disc bearing disc 131. Therefore, the moving path of the transfer of the reaction cup 2 can be reduced, the detection efficiency is improved, the space occupied by the incubation device 1 can be greatly reduced, the internal space of the chemiluminescent detector is saved, and the whole volume is further reduced. Of course, in other embodiments of the present invention, the buffer carrier plate, the reaction carrier plate 121, and the reaction inner plate carrier plate 131 are separately provided. It is understood that the buffer carrier plate, the reaction carrier plate 121 and the reaction inner plate carrier plate 131 may be arranged in a straight line, a delta shape or other forms, so long as the operations on the buffer carrier plate, the reaction carrier plate 121 and the reaction inner plate carrier plate 131 can be performed independently, the operation time of the incubation device 1 of the present invention can be shortened, and the purpose of improving the detection efficiency can be achieved.
Further, the axis of the reaction carrier disk 121 coincides with the axis of the reaction inner disk carrier disk 131. It will be appreciated that when the reaction carrier plate 121 is sleeved on the outer side of the reaction inner plate carrier plate 131, the axis of the reaction carrier plate 121 coincides with the axis of the reaction inner plate carrier plate 131, so that the space occupied by the reaction carrier plate 121 and the reaction inner plate carrier plate 131 can be reduced, and the volume of the incubation device 1 can be reduced.
Still further, the top surface of the reaction carrier plate 121 is in the same plane as the top surface of the reaction inner plate carrier plate 131. The transfer of reaction cup 2 can be facilitated, interference between reaction cup 2 and the top surface of reaction bearing disc 121 or the top surface of reaction inner disc bearing disc 131 during transfer is avoided, the reliability of transfer of reaction cup 2 is ensured, and the usability of the chemiluminescent detector is further ensured. Of course, in other embodiments of the invention, the top surface of the reaction plate 121 is in a plane lower or higher than the top surface of the reaction plate 131.
The invention also provides a chemiluminescent detector which comprises an automatic transmission device of the reaction cup 2, a sample adding device, a cleaning device, a luminescent detection device, a control device and the incubation device 1 in the embodiment. The control device transfers the reaction cup 2 in the automatic reaction cup 2 box conveying device into the incubation device 1, the control device controls the sample adding device to add samples to the reaction cup 2 in the incubation device 1, and after incubation is completed, the control device controls the reaction cup 2 to be sequentially transferred into the cleaning device and the luminescence detection device. According to the chemiluminescent detector disclosed by the invention, the incubation operation steps of the sample can be carried out in a zoned manner through the incubation device 1, so that the functions of the incubation device 1 are organically distributed, each step can be carried out simultaneously, the problem that the operation time is long due to the fact that each step of the traditional sample reaction occupies time and occupies a large space when the steps are carried out is solved, the processing time of the sample on the incubation device 1 is shortened, the processing efficiency of the sample is improved, the incubation processing efficiency of the sample is further improved, and the detection efficiency of the whole chemiluminescent detector is improved. Meanwhile, the arrangement of the incubation device 1 can save space, so that the incubation device 1 can realize high-speed and high-precision operation on the premise of not occupying excessive space, and simultaneously, the huge production of the incubation device 1 and the whole machine can be avoided, so that the whole machine size of the chemiluminescent detector is ensured.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be regarded as the description scope of the present specification.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (24)
1. A reaction disk mechanism, comprising:
a reaction disk mounting structure;
the reaction disc driving structure is arranged on the reaction disc mounting structure;
the reaction bearing disc is arranged on the reaction disc driving structure, and the reaction disc driving structure can drive the reaction bearing disc to do rotary motion; the reaction bearing plate can accommodate reaction cups, a plurality of placing holes for placing the reaction cups are formed in the reaction bearing plate, the placing holes are uniformly distributed along the circumferential direction of the reaction bearing plate, and the reaction cups added with samples can be respectively transferred into the placing holes; and
The guiding and limiting structure can conduct guiding and limiting on the rotary motion of the reaction bearing disc, and comprises a guiding and limiting sliding rail and a rolling support piece, wherein the guiding and limiting sliding rail is arranged on the reaction bearing disc, and the guiding and limiting sliding rail protrudes out of the outer peripheral surface of the reaction bearing disc; the rolling support piece is arranged on the reaction disc mounting structure and positioned on the periphery of the reaction bearing disc, and the guide limiting sliding rail is slidably matched with the rolling support piece.
2. The reaction plate mechanism of claim 1 wherein the rolling support is a guide limit bearing, a chute is formed in an outer ring of the guide limit bearing, and the guide limit slide rail is slidably located in the chute.
3. The reaction plate mechanism of claim 2, wherein the cross-sectional shape of the portion of the guide limit slide rail located in the slide groove is V-shaped, and the cross-sectional shape of the slide groove is V-shaped.
4. The reaction plate mechanism of claim 2, wherein the cross-sectional shape of the portion of the guide limit slide rail located in the slide slot is adapted to the cross-sectional shape of the slide slot.
5. The reaction disk mechanism of claim 3 wherein the guide limiting structure further comprises a lubrication member disposed on the reaction disk mounting structure and located on a peripheral side of the reaction carrier disk, the guide limiting slide rail being capable of contacting the lubrication member.
6. The reaction disk mechanism of claim 5 wherein the lubrication component has a lubrication groove, the guide limit slide rail is located in the lubrication groove, and the lubrication groove is also V-shaped in cross-section.
7. The reaction disk mechanism of claim 2 wherein the number of rolling supports is at least three, the at least three rolling supports being evenly distributed around the perimeter of the reaction carrier disk.
8. The reaction plate mechanism of any one of claims 1 to 7 further comprising a compression structure disposed on the reaction plate mounting structure, wherein one of the rolling supports is mounted on the compression structure for adjusting a spacing between the rolling support and the guide limit rail.
9. The reaction disk mechanism according to claim 8, wherein the pressing structure comprises a pressing mounting seat, a pressing elastic member and a pressing guide rod, the pressing guide rod is mounted on the reaction disk mounting structure, the pressing mounting seat is slidably arranged on the pressing guide rod, the pressing elastic member is sleeved on the pressing guide rod, and two ends of the pressing elastic member are respectively abutted against the pressing guide rod and the pressing mounting seat;
the rolling support piece is installed on the compaction installation seat, and the compaction elastic piece can enable the compaction installation seat to move on the compaction guide rod and be abutted to the guide limiting sliding rail.
10. The reaction disk mechanism of claim 9 wherein the compression structure further comprises a compression mount fixed to the reaction disk mounting structure, the compression guide bar being mounted to the compression mount.
11. The reaction tray mechanism of claim 10, wherein the reaction tray mounting structure has a plurality of reaction stations including a cup adding station, a reagent adding station, a mixing station, and an incubation cup taking station arranged in sequence;
The cup adding station is arranged corresponding to a cup grabbing mechanism, and the cup grabbing mechanism can place the reaction cup with the added sample into the placing hole of the reaction bearing disc at the cup adding station;
the reagent adding station is arranged corresponding to a reagent adding device, and the reagent adding device can add reagent into the reaction cup of the reaction bearing disc in the reagent adding station;
the mixing station is arranged corresponding to a mixing device, and the mixing device can mix samples in the reaction cup of the reaction bearing disc with reagents uniformly in the mixing station;
the incubation cup taking station is arranged corresponding to the cup grabbing mechanism, and the cup grabbing mechanism can grab the reaction cups in the reaction bearing disc at the incubation cup taking station and transfer the reaction cups to the reaction disc mechanism.
12. The reaction disk mechanism of claim 11 wherein the reaction station further comprises a wash and place cup station, the wash and place cup station being located between the cup adding station and the reagent adding station.
13. The reaction disk mechanism of claim 12 wherein the reagent adding station comprises a first reagent adding station and a second reagent adding station, the first reagent adding station and the second reagent adding station being located between the purge cup placing station and the mixing station.
14. The reaction disk mechanism of any one of claims 11 to 13 wherein the reaction disk mounting structure further has a buffer station located between any adjacent two reaction stations, the buffer station being capable of equalizing the spacing between adjacent two reaction stations and between adjacent reaction stations and buffer stations.
15. The reaction disk mechanism of claim 13, wherein the reaction disk mounting structure further comprises a buffer station, the buffer station comprises a first buffer station, a second buffer station and a third buffer station, and the cup adding station, the first buffer station, the cleaning and cup placing station, the second buffer station, the first reagent adding station, the second reagent adding station, the mixing station, the incubation cup taking station and the third buffer station are sequentially arranged at equal intervals along the circumferential direction of the reaction carrier disk.
16. The reaction disk mechanism of claim 15 wherein the number of placement holes on the reaction carrier disk is an integer multiple of the sum of the number of reaction stations and the buffer station.
17. The reaction disk mechanism according to claim 1, wherein the reaction disk driving structure comprises a reaction driving motor and a reaction transmission assembly, the reaction driving motor is mounted on the reaction disk mounting structure, the reaction transmission assembly is in transmission connection with the output end of the reaction driving motor and the reaction bearing disk, and the reaction driving motor drives the reaction transmission assembly to drive the reaction bearing disk to rotate;
the reaction transmission assembly comprises a reaction driving wheel and a reaction synchronous belt, the reaction driving wheel is arranged at the output end of the reaction driving motor, the reaction synchronous belt is sleeved on the reaction driving wheel and the reaction bearing disc, the driving motor drives the reaction driving wheel to rotate, and the reaction bearing disc is driven to rotate through the reaction synchronous belt.
18. The reaction disk mechanism of claim 17 further comprising a tensioning structure disposed on the reaction disk mounting structure, the tensioning structure abutting the reaction timing belt.
19. The reaction plate mechanism of claim 18, wherein the tensioning structure comprises a tensioning wheel, a tensioning wheel shaft, a tensioning guide rod and a tensioning elastic piece, the tensioning guide rod is fixed on the reaction plate mounting structure, the tensioning wheel is rotatably arranged on the tensioning wheel shaft, the tensioning wheel is located on the outer side of the reaction synchronous belt, one end of the tensioning elastic piece is fixed on the tensioning guide rod, the other end of the tensioning elastic piece is connected with the tensioning wheel shaft, and the tensioning elastic piece can move along the tensioning guide rod to enable the tensioning wheel to be abutted against the reaction synchronous belt.
20. The reaction plate mechanism of claim 19, wherein the tensioning structure further comprises a tensioning slide rail and a tensioning slide block, the tensioning slide rail is arranged on the reaction plate mounting structure, the tensioning wheel shaft is fixed on the tensioning slide block, the tensioning slide block is slidably arranged on the tensioning slide rail, the tensioning elastic piece is connected with the tensioning slide block, and the tensioning elastic piece can drive the tensioning slide block to move along the tensioning slide rail and drive the tensioning wheel shaft and the tensioning wheel thereon to move.
21. The reaction plate mechanism of claim 20 wherein the tensioning mechanism further comprises a tensioning connection member that connects the tensioning slide and the tensioning spring, the tensioning spring moving the tensioning slide through the tensioning connection member.
22. An incubation apparatus comprising a buffer tray means, a reaction inner tray means and a reaction tray means as claimed in any one of claims 1 to 21;
the buffer disc mechanism comprises a rotatable buffer bearing disc, wherein the buffer bearing disc can accommodate a reaction cup and can enable the reaction cup to finish sample adding operation on the buffer bearing disc;
The reaction bearing disc of the reaction disc mechanism can accommodate the reaction cup transferred from the buffer disc mechanism and can enable the reaction cup to finish the reagent adding operation and the uniform mixing operation on the reaction disc mechanism;
the reaction inner disc mechanism comprises a rotatable reaction inner disc bearing disc, wherein the reaction inner disc bearing disc can contain the reaction cup transferred from the reaction bearing disc and can enable the reaction cup to perform incubation operation on the reaction inner disc mechanism;
the reaction bearing disc and the reaction inner disc bearing disc can rotate independently.
23. The incubation device of claim 22 wherein the reaction tray is circular and the reaction inner tray is disk-shaped;
the reaction bearing disc is sleeved on the outer side of the reaction inner disc bearing disc, and the buffer bearing disc is independent of the reaction bearing disc.
24. A chemiluminescent detector comprising a cuvette automatic transfer device, a sample addition device, a washing device, a luminescent detection device, a control device, and an incubation device according to any one of claims 22-23;
the control device transfers the reaction cups in the automatic reaction cup conveying device to the incubation device, controls the sample adding device to add samples to the reaction cups in the incubation device, and controls the reaction cups to be sequentially transferred to the cleaning device and the light-emitting detection device after incubation is completed.
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| CN114965886B (en) * | 2022-05-18 | 2023-11-03 | 深圳无疆生命科学有限公司 | Sample loading tray device and sample analyzer |
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