CN203825022U - Full-automatic chemiluminescence immunoassay analyzer - Google Patents
Full-automatic chemiluminescence immunoassay analyzer Download PDFInfo
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- CN203825022U CN203825022U CN201320829657.XU CN201320829657U CN203825022U CN 203825022 U CN203825022 U CN 203825022U CN 201320829657 U CN201320829657 U CN 201320829657U CN 203825022 U CN203825022 U CN 203825022U
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Abstract
The utility model provides a full-automatic chemiluminescence immunoassay analyzer. The full-automatic chemiluminescence immunoassay analyzer comprises a rack, and a sampling device, a reaction control device and an optical detection assembly which are arranged on the rack, wherein the sampling device comprises a reaction cup array mechanism, a reaction cup delivery rail, a sampling gun head array mechanism, a reagent storage tray, a reagent filling rail, a sample conveying device, a sample storage tray, a first mechanical arm, a second mechanical arm, a third mechanical arm, a sampling filling needle, a first reagent filling needle and a second reagent filling needle; the reaction control device comprises a reaction cup incubating tray, a primary magnetic separation rail, a secondary magnetic separation rail, a first cleaning and magnetic separation mechanism, a second cleaning and magnetic separation mechanism and a substrate filling mechanism; and the optical detection assembly comprises a photomultiplier and a control circuit. The full-automatic chemiluminescence immunoassay analyzer can automatically perform operations such as sample loading, reaction, data reading and the like in the chemiluminescence immunoassay testing flow.
Description
Technical field
The utility model relates to a kind of Full-automatic chemiluminescence immune detection analyser, is widely used in the detection analysis to hormone, virus marker thing, tumor markers and medicine of clinical labororatory and R&D institution, belongs to biochemistry detection equipment technical field.
Background technology
Immunology detection is mainly a kind of method of utilizing the specific reaction of antigen and antibody to detect, because it can utilize isotope, enzyme, chemiluminescent substance etc., detection signal amplified and shown, being therefore often used to detect the micro substance such as protein, hormone.Since the sixties in last century, immunoassay is just widely used in scientific research and clinical field.Progressively develop into euzymelinked immunosorbent assay (ELISA) until the chemiluminescence immunoassay method of widespread use at present from the radioimmunology starting most.
Chemiluminescence immune assay is that chemiluminescence or bioluminescence are combined with immune response, for detection of a kind of novel markings immunoassay of trace antigen or antibody.Chemical illumination immunity analysis instrument comprises two parts, i.e. immune response system and chemiluminescence analysis system.Chemiluminescence analysis system is to utilize chemiluminescent substance through the catalysis of catalyzer and the oxidation of oxygenant, form the intermediate of an excited state, in the time that this excited state intermediate is got back to stable ground state, launch photon simultaneously, utilize luminous signal surveying instrument to measure quantum yield of luminscence.Immune response system is that luminescent substance (exciting lower generation excited state intermediate at reactant) is directly marked on antigen (chemiluminescence immune assay) or antibody (immunochemiluminometry), or enzyme acts on luminous substrate.In chemical illumination immunity analysis instrument, core sensitive detection parts are photomultiplier, it is the vacuum electron device that faint optical signal can be converted to electric signal, by singl e photon detection and transfer to amplifier, and the electric current that adds high pressure amplifies, analog current is converted into digital current by amplifier, digital current is transferred to luminous signal computer and is calculated by data line, draws clinical effectiveness.Radio-immunity environmental protection, safety that Chemiluminescence Immunoassay is more traditional, highly sensitive compared with enzyme linked immunological, sensing range is wide.Chemiluminescence immunoassay detects environmental protection, the feature such as quick, accurate and has obtained people's generally accreditation, has become the Main Means of current clinical immunodiagnosis.
Nonetheless, chemiluminescence immune assay also has its restricted gender.The uncertainty of chemiluminescence immunoassay testing result comes from the impact of experimental implementation and detection system.In recent years, along with the progress of scientific and technological level, the stability, the consistance that detect reagent and testing instruments also progressively improve, and the principal element that affects chemiluminescence detection result is exactly experimental implementation.Testing agency takes the mode that artificial experimental implementation or the luminous detector of semi-automatic chemistry detect mostly at present, and detecting instrument only has single read functions.There is individual difference in manual operation, not only affects the accuracy of result, also limited the flux detecting in the time that sample size is various.How can provide automation experiment flow operations, high-throughout chemiluminescence detector device to become the important topic that people face.
Summary of the invention
The purpose of this utility model is to provide a kind of Full-automatic chemiluminescence immunoassay analysis meter, and it can automatically complete application of sample, reaction, data on chemiluminescence immunoassay inspection process and the sequence of operations such as read.
Specifically, Full-automatic chemiluminescence immunoassay analysis meter described in the utility model, comprises frame and is arranged on sampling device, Reaction Control Assembly and the optical detection assembly in frame,
-sampling device comprises reaction cup array mechanism, reaction cup transmission track, sample loading gun head (TIP) array mechanism, reagent storage dish, reagent filling track, sample conveying device, sample storage dish, the first mechanical arm, the second mechanical arm, the 3rd mechanical arm, sample filling pin, the first reagent filling pin and the second reagent filling pin;
-reaction control section comprises that reaction cup incubation dish, a magnetic separate track, secondary magnetic separation track, the first cleaning and magnetic separator structure, the second cleaning and magnetic separator structure and substrate filling mechanism;
-optical detection assembly comprises photomultiplier and control circuit.
Further, described reaction cup incubation dish comprises inner disc and outer disk, is rotated respectively by driven by motor independently, is respectively equipped with a plurality of reaction cup holes for placing response cup in inner disc and outer disk, and in reaction cup incubation dish, be provided with attemperating unit, can carry out incubation, insulation.
Further, described reagent filling track comprises support and is arranged on the closed orbit by driven by motor on support, moves in orbit that approach is provided with successively the first reagent adding position, the second reagent adding position, mixes position, reaction cup is grabbed into position and reaction cup and caught position along reaction cup.
Further, described sample conveying device comprises that the sample rack injection and the sample rack that are driven by motor and travelling belt respectively reclaim track.
Further, described sample storage dish, reagent storage dish and reaction cup incubation dish are positioned at the middle part of frame, for adding the reaction cup array mechanism of reaction cup, for adding the sample loading gun head array mechanism of sample loading gun head, for adding the sample conveying device of sample, one time magnetic separates track, secondary magnetic separates track, substrate filling mechanism and optical detection assembly are arranged in respectively the edge of frame, one time magnetic separates other the first cleaning and the magnetic separator structure of being provided with of track, secondary magnetic separates other the second cleaning and the magnetic separator structure of being provided with of track, the end that separates track at secondary magnetic is provided with optical detection assembly, reagent filling track is arranged on by the reagent storage dish of central rack, the first mechanical arm, the second mechanical arm and the 3rd mechanical arm lay respectively at sample storage dish, reagent storage dish and reaction cup incubation dish top, sample filling pin is positioned at sample storage dish top, the first reagent filling pin and the second reagent filling pin lay respectively at reagent storage dish top.
Further, described reaction cup array mechanism and sample loading gun head array mechanism can screen reaction cup and sample loading gun head and array, reaction cup and sample loading gun head are piled up neatly as requested, and reaction cup transmission track is for being transported to specific position by reaction cup, for use.
Further, described sample introduction part can be carried out the permutation of sample automatically, is sent to sample position to be taken, and the recovery of residue sample, and this sample introduction part also comprises urgent sample load port in addition; If while having many instruments to carry out parallel running, sample can be sent to each application of sample position for subsequent use; Sample storage dish, for sample is stored and backed up, for the test of multiple disparity items, can also store the sample that need to retest; The first mechanical arm is responsible for reaction cup grabbing into and catching at ad-hoc location in sample warehouse.
Further, described reagent storage dish is for carrying out the Storage and Processing of reagent, and it can provide cold storage environment guarantee reagent standing storage to undergo no deterioration; Reagent filling track is for the filling of reagent and mixing of reactant, the first reagent filling pin and the second reagent filling pin can, by corresponding reagent being added in corresponding reaction cup of accurate quantification respectively, mix at the ad-hoc location of reagent filling track after adding; The second mechanical arm is responsible for reaction cup grabbing into and catching at ad-hoc location on reagent filling track.
Further, described reaction cup incubation dish is responsible for reaction cup to carry out incubation under specific temperature environment, and incubation is caught reaction cup at ad-hoc location the processing of carrying out next step after special time by the 3rd mechanical arm; This incubation dish is divided into inner disc and outer disk, and internal and external disk can carry out incubation according to the specific time respectively; This incubation dish has accurate temperature control equipment, ensures that the temperature of incubation keeps stable; The 3rd mechanical arm is responsible for reaction cup the grabbing into and catching at ad-hoc location in internal and external disk.
Further, a described magnetic separates track for reaction cup is transmitted between sample storage dish, reagent storage dish, reaction cup incubation dish; Carry out grabbing into and catching of reaction cup by three groups of mechanical grippers in specific position respectively; One time magnetic separates the position that also includes the first cleaning and magnetic separator structure above track, separates with magnetic for the primary cleaning of course of reaction.
Further, described secondary magnetic separates track for reaction cup is transmitted between reaction cup incubation dish, the second cleaning and magnetic separator structure, substrate filling mechanism and optical detection assembly; What the 3rd mechanical arm was responsible for carrying out reaction cup on fixed position grabs into and catches action; The second cleaning and magnetic separator structure separate at secondary magnetic on the fixed position of track, and secondary cleaning of being responsible in course of reaction separates with magnetic; Substrate filling mechanism separates on the fixed position of track at secondary magnetic, is responsible for carrying out substrate filling after secondary cleaning has separated with magnetic; Described optical detection assembly is responsible for detecting luminous intensity after substrate has been annotated.
Known in the field, described analyser can comprise supporting with it computing machine and operation system of software, to realize reading and analyzing of computing machine.
The advantage of Full-automatic chemiluminescence immunoassay analysis meter described in the utility model and good effect are:
1, automaticity is high, user-friendly.The present invention adopts full-automatic sample introduction, enters cup, enters sample loading gun head, and full-automatic waste recovery design has not substantially had artificial participation process in test process, makes to control simpler;
2, reagent adding mode more flexibly.The present invention adopts independently reagent adding track structure, can add multiple different reagent simultaneously, also supports that reagent mixes simultaneously, makes reaction more abundant, and result is more accurate;
3, large flux, many test volumes carry out simultaneously.Reaction cup incubation dish adopts inside and outside two coil structures, can hold a hundreds of test simultaneously, and incubative time can be adjusted flexibly between Internal and external cycle;
4, have that function is strong, system is flexible.System is supported a step, and the multiple testing processs such as two steps and three steps can be processed multinomial test flexibly according to the requirement of test, adapt to the test request of different Clinical Projects.
Brief description of the drawings
Fig. 1 is the one-piece construction schematic diagram of the embodiment of the present invention;
Fig. 2 is the reagent adding track structure schematic diagram of the embodiment of the present invention;
Fig. 3 is the reaction cup incubation dish structural representation of the embodiment of the present invention;
Fig. 4 is the workflow schematic diagram of the embodiment of the present invention;
Wherein, 1 is reaction cup array mechanism, 2 is reaction cup transmission track, 3 is sample loading gun head array mechanism, 4 is reagent storage dish, 5 is reagent filling track, 6 is sample conveying device, 7 is sample storage dish, 8 is the first mechanical arm, 9 is the second mechanical arm, 10 is the 3rd mechanical arm, 11 is sample filling pin, 12 is the first reagent filling pin, 13 is the second reagent filling pin, 14 reaction cup incubation dishes, 15 is that a magnetic separates track, 16 is secondary magnetic separation track, 17 is the first cleaning and magnetic separator structure, 18 is the second cleaning and magnetic separator structure, 19 is substrate filling mechanism, 20 is optical detection assembly, 21 is sample tube, 22 is frame, 50 is support, 51 first reagent adding positions, 52 is the second reagent adding position, 53 for mixing position, 54 grab implantation site for reaction cup, 55 catch position for reaction cup, 61 is sample rack injection, 62 is sample rack recovery track, 63 is urgent sample load port, 141 is inner disc, 142 is outer disk.
Embodiment
Below in conjunction with accompanying drawing, Full-automatic chemiluminescence immunoassay analysis meter of the present invention is described further, object is for the public understands described technology contents better, and the not restriction to described technology contents.
A specific embodiment of complete machine structure of the present invention as shown in Figure 1, comprise frame 22 and be arranged on sample introduction part, reaction control section and the optical detection assembly in frame 22, sample introduction part comprises reaction cup array mechanism 1, reaction cup transmission track 2, sample loading gun head array mechanism 3, reagent storage dish 4, reagent filling track 5, sample conveying device 6, sample storage dish 7, the first mechanical arm 8, the second mechanical arm 9, the 3rd mechanical arm 10, sample filling pin 11, the first reagent filling pin 12 and the second reagent filling pin 13; Reaction control section comprises that reaction cup incubation dish 14, magnetic separate that track 15, secondary magnetic separate that track 16, first cleans and magnetic separator structure 17, second cleans and magnetic separator structure 18 and substrate filling mechanism 19 and optical detection assembly 20 form, and optical detection assembly 20 comprises photomultiplier and control circuit.In the present embodiment, described sample storage dish 7, reagent storage dish 4 and reaction cup incubation dish 14 are positioned at the middle part of frame 22, for adding the reaction cup array mechanism 1 of reaction cup, for adding the sample loading gun head array mechanism 3 of sample loading gun head, for adding the sample conveying device 6 of sample, one time magnetic separates track 15, secondary magnetic separates track 17, substrate filling mechanism 19 and optical detection assembly 20 are arranged in respectively the edge of frame 22, one time magnetic separates other the first cleaning and the magnetic separator structure 16 of being provided with of track 15, secondary magnetic separates other the second cleaning and the magnetic separator structure 18 of being provided with of track 17, the end that separates track 17 at secondary magnetic is provided with optical detection assembly 20, reagent filling track 5 is arranged on frame 22 middle parts, the first mechanical arm 8, the second mechanical arm 9 and the 3rd mechanical arm 10 lay respectively at sample storage dish 7, reagent storage dish 4 and reaction cup incubation dish 14 tops, sample filling pin 11 is positioned at sample storage dish 7 tops, the first reagent filling pin 12 and the second reagent filling pin 13 lay respectively at reagent storage dish 4 tops, sample conveying device 6 comprises that the sample rack injection 61 and the sample rack that are driven by motor and travelling belt respectively reclaim track 62, and be also provided with urgent sample load port 63 in one end of sample conveying device, be convenient to carry out the detection of emergency treatment sample.
In Fig. 1, described reaction cup array mechanism 1 and sample loading gun head array mechanism 3 can screen reaction cup and sample loading gun head and array, and reaction cup and sample loading gun head are piled up neatly as requested; Reaction cup transmission track 2 is for reaction cup being transported to specific position, for use.
In Fig. 1, described sample introduction part can be carried out the permutation of sample automatically, is sent to sample position to be taken, and the recovery of residue sample, and this sample introduction part also comprises the " loaded " position of urgent test sample book in addition; If while having many instruments to carry out parallel running, sample can be sent to each application of sample position for subsequent use; Sample storage dish 7, for sample is stored and backed up, for the test of multiple disparity items, can also store the sample that need to retest; The first mechanical arm 8 is responsible for reaction cup grabbing into and catching at ad-hoc location in sample warehouse.
In Fig. 1 and Fig. 2, described reagent storage dish 4 is for carrying out the Storage and Processing of reagent, and it can provide cold storage environment guarantee reagent standing storage to undergo no deterioration; Reagent filling track 5 is for the filling of reagent and mixing of reactant, comprise support 50, the first reagent filling pin 12 can be by corresponding reagent respectively in the reaction cup that is added to the first reagent adding position 51 of accurate quantification, the second reagent filling pin 13 can be by corresponding reagent respectively in the reaction cup that is added to the second reagent adding position 52 of accurate quantification, adding after, mixes mixing on 53 of reagent filling track 5; The second mechanical arm 9 is responsible for grabbing implantation site 54 in reaction cup and is grabbed into reaction cup, catches position 55 catch reaction cup in reaction cup.
In Fig. 1 and Fig. 3, described reaction cup incubation dish 14 is responsible for reaction cup to carry out incubation under specific temperature environment, and incubation is caught reaction cup at ad-hoc location the processing of carrying out next step after special time by the 3rd mechanical arm 10; This incubation dish is divided into inner disc 141 and outer disk 142, and internal and external disk can carry out incubation according to the specific time respectively; This incubation dish has accurate temperature control equipment, ensures that the temperature of incubation keeps being stabilized in 37 DEG C; Reaction cup the grabbing into and catching at ad-hoc location that the 3rd mechanical arm 10 is responsible in internal and external disk.
In Fig. 1, a described magnetic separates track 15 for reaction cup is transmitted between sample storage dish 7, reagent storage dish 4, reaction cup incubation dish 14; Carry out grabbing into and catching of reaction cup by three groups of mechanical grippers in specific position respectively; One time magnetic separates the position that also includes the first cleaning and magnetic separator structure 17 above track 15, separates with magnetic for the primary cleaning of course of reaction.
In Fig. 1, described secondary magnetic separates track 16 for reaction cup is transmitted between reaction cup incubation dish 14, the second cleaning and magnetic separator structure 18, substrate filling mechanism 19 and optical detection assembly 20; What the 3rd mechanical arm 10 was responsible for carrying out reaction cup on fixed position grabs into and catches action; The second cleaning and magnetic separator structure 18 separate at secondary magnetic on the fixed position of track 16, and secondary cleaning of being responsible in course of reaction separates with magnetic; Substrate filling mechanism 19 separates on the fixed position of track 16 at secondary magnetic, is responsible for carrying out substrate filling after secondary cleaning has separated with magnetic; In Fig. 1, described optical detection assembly 20, is responsible for detecting luminous intensity after substrate has been annotated.
Analyser also needs one group of hardware control circuit and controls software, for controlling and commanding each part mentioned above mechanism to need coordinated work according to chemiluminescence immune assay.
The remaining part of the present utility model of not mentioning in the above description and device are this area routine techniquess.
In the instrument course of work, the action of each parts is undertaken by instrument workflow, and Fig. 4 is the workflow diagram of full-automatic chemical immunoluminescence analytical instrument of the present invention, and package unit both can have been supported single stage method test in conjunction with this flow process, also can support two-step approach test, even three-step approach test.Whole instrument concrete operation step is as follows:
A. instrument start, system initialization: reaction cup and sample loading gun head automatically enter respectively reaction cup display mechanism 1 and sample loading gun head display mechanism 3 carries out array arrangement, the sample rack that is placed with sample tube through sample transporting rail 6 through being delivered to preposition, in sample tube, be loaded with sample, all mechanical motion structures reset; The optical detection background value of testing oneself, background value is up to standard; After reaction cup incubation dish 14 reaches 37 DEG C of constant temperature, system just allows to start test;
B. add sample, automatic dilution: the first mechanical arm 8 grabs reaction cup in sample storage dish 7 by reaction cup transmission track 2, sample filling pin 11 is drawn sample and adds in the reaction cup of sample storage dish 7 from sample tube, if sample needs dilution, with the first reagent filling pin 12, the dilution in reagent storage dish 4 is added in reaction cup simultaneously and is mixed; The first mechanical arm 8 grabs a magnetic by the reaction cup that adds sample from sample storage dish 7 again and separates track 15;
C. reagent adding, reaction cup mixes: a magnetic separates track 15 and reaction cup can be sent to predetermined reagent orbital position, the second mechanical arm 9 separates track 15 by the reaction cup that adds sample from a magnetic again and grabs reagent filling track 5, reaction cup is sent to reagent adding position by reagent filling track 5, the first reagent filling pin 12 and the second reagent filling pin 13 join required reagent in reaction cup, reaction cup is sent to and mixes position and mix, reaction cup is sent to and catches position, the second mechanical arm 9 grabs a magnetic by reaction cup from reagent filling track 5 again and separates track 15, be sent to the first cleaning and separating mechanism 17 cleans separation,
D. reaction cup incubation a: magnetic separates track 15 and reaction cup can be sent to reaction cup incubation dish 14 positions, the 3rd mechanical arm 10 grabs reaction cup in reaction cup incubation dish 14 and hatches, and the 3rd mechanical arm 10 can be according to the needed incubative time of system mobile response cup between the internal and external disk of reaction cup incubation dish 14;
E. cleaning magnetic separates: after having hatched, the 3rd mechanical arm 10 catches reaction cup again, if two-step approach or three-step approach, reaction cup will be caught a magnetic and be separated track 15, then re-start the operation of above-mentioned c, d step, if single-step process, reaction cup will be caught secondary magnetic and be separated track 16; Reaction cup is taken to the second cleaning by secondary magnetic separation track 16 and magnetic separator structure 18 cleans separation;
F. add substrate, Photoelectric Detection: then reaction cup is separated track 16 by secondary magnetic and delivers to the position that adds substrate, substrate filling mechanism 19 adds substrate in reaction cup, mixes reaction cup; Reaction cup is separated track 16 by secondary magnetic and takes Photoelectric Detection position to, and optical detection assembly 20 detects luminous value, after having detected, abandons reaction cup;
G. Output rusults: calculate and export measurement result.
Should be clear and definite be; the above embodiments comprise word and accompanying drawing; it is only a specific embodiment of described technical scheme; in fact; all improvement and conversion of described technical scheme being carried out with identical or approximate principle; comprise the variation of the position relationship of shape, the size of complete machine and each several part structure, material used, arrangement, and the close element of function be equal to replacement, all within the utility model technical scheme required for protection.
Claims (5)
1. a Full-automatic chemiluminescence immunoassay analysis meter, is characterized in that: comprises frame and is arranged on sampling device, Reaction Control Assembly and the optical detection assembly in frame,
-sampling device comprises reaction cup array mechanism, reaction cup transmission track, sample loading gun head array mechanism, reagent storage dish, reagent filling track, sample conveying device, sample storage dish, the first mechanical arm, the second mechanical arm, the 3rd mechanical arm, sample filling pin, the first reagent filling pin and the second reagent filling pin;
-reaction control section comprises that reaction cup incubation dish, a magnetic separate track, secondary magnetic separation track, the first cleaning and magnetic separator structure, the second cleaning and magnetic separator structure and substrate filling mechanism;
-optical detection assembly comprises photomultiplier and control circuit.
2. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: described reaction cup incubation dish comprises inner disc and outer disk, rotated by driven by motor independently respectively, in inner disc and outer disk, be respectively equipped with a plurality of reaction cup holes for placing response cup, and be provided with attemperating unit in reaction cup incubation dish.
3. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1 and 2, it is characterized in that: described reagent filling track comprises support and be arranged on the closed orbit by driven by motor on support, move along reaction cup in orbit that approach is provided with successively the first reagent adding position, the second reagent adding position, mixes position, reaction cup is grabbed into position and reaction cup and caught position.
4. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1 and 2, is characterized in that: described sample conveying device comprises that the sample rack injection and the sample rack that are driven by motor and travelling belt respectively reclaim track.
5. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1 and 2, it is characterized in that: described sample storage dish, reagent storage dish and reaction cup incubation dish are positioned at the middle part of frame, for adding the reaction cup array mechanism of reaction cup, for adding the sample loading gun head array mechanism of sample loading gun head, for adding the sample conveying device of sample, one time magnetic separates track, secondary magnetic separates track, substrate filling mechanism and optical detection assembly are arranged in respectively the edge of frame, one time magnetic separates other the first cleaning and the magnetic separator structure of being provided with of track, secondary magnetic separates other the second cleaning and the magnetic separator structure of being provided with of track, the end that separates track at secondary magnetic is provided with optical detection assembly, reagent filling track is arranged on by the reagent storage dish of central rack, the first mechanical arm, the second mechanical arm and the 3rd mechanical arm lay respectively at sample storage dish, reagent storage dish and reaction cup incubation dish top, sample filling pin is positioned at sample storage dish top, the first reagent filling pin and the second reagent filling pin lay respectively at reagent storage dish top.
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