CN210401443U

CN210401443U - High-flux full-automatic immune luminescence analysis system

Info

Publication number: CN210401443U
Application number: CN201920779686.7U
Authority: CN
Inventors: 何宗平
Original assignee: Hefei Yuntao Optoelectronics Technology Co ltd
Current assignee: Fosun diagnostic technology (Hefei) Co.,Ltd.
Priority date: 2019-05-27
Filing date: 2019-05-27
Publication date: 2020-04-24
Anticipated expiration: 2029-05-27

Abstract

The utility model discloses a high-flux full-automatic immune luminescence analysis system, which comprises an application of sample bracket, an application of sample arm, an application of sample needle, a reagent bin, a sample bin, a reaction incubation disc, a reaction turntable, a reaction cup groove, a reaction cup automatic cup loading component, a reaction cup clamping arm, a cleaning station component, a heat preservation disc, a heat preservation turntable, a substrate needle, a measurement darkroom component and a photon measurement component; a plurality of sample adding arms are used for simultaneously adding samples or reagents, so that the number of reaction cups which are added with the samples or the reagents in unit time is increased, and the flux of the system is increased; according to the requirement, a substrate needle can be arranged outside the measurement darkroom component or the measurement darkroom component to adapt to the measurement requirement of a flash method or a glow method, the reaction cup added with the substrate can be delayed and insulated according to the requirement under the condition that the substrate is added outside, the photon measurement component can measure the product in the reaction cup, and the computer can calculate the concentration of the object to be measured according to the measurement result.

Description

High-flux full-automatic immune luminescence analysis system

Technical Field

The utility model relates to a biological monitoring and clinical examination equipment technical field specifically are a full-automatic immune luminescence analysis system of high flux.

Background

With the development of scientific technology, chemiluminescence, which uses light excited during a chemical reaction, and bioluminescence, which uses light generated by enzymatic catalysis during a chemical reaction, and measurement of chemiluminescence, which has become common in recent years, have been used to determine the content of an unknown component in a sample to be measured, and have also played an important role in the study of gene expression and regulation in the past decade. Compared with other measurement technologies, the chemical and biochemical luminescence measurement technologies have the following advantages: extremely high sensitivity, wide dynamic range and continuous emergence of luminescence measuring reagent. The luminescence measurement has an extremely high sensitivity, which is 10 higher than that of the spectral absorption measurement technique⁵The power is at least 1000 times higher than that of the fluorescence measurement technology.

However, the conventional automatic immune luminescence analyzer increases the stroke of the sample adding arm by using an XYZ motion mechanism, so that the number of cuvettes to which a sample or a reagent is added is relatively low in a unit time, and the sample processing speed is relatively slow.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a full-automatic immune luminescence analysis system of high flux, the problem that proposes to the background art is further improved, utilizes a plurality of application of sample arms to carry out sample or reagent interpolation simultaneously, improves the reaction cup quantity that has added sample or reagent in the unit interval, realizes that the reaction cup quantity that has added sample and reagent in every hour reaches 720 times at the fastest to improve the flux of system; meanwhile, a substrate needle can be arranged outside the measurement darkroom component or the measurement darkroom component according to the requirement so as to adapt to the measurement requirement of a flash method or a glow method; under the condition of adding the substrate externally, the system after adding the substrate can be delayed and insulated according to the requirement, the photon measuring component can measure the product in the reaction cup, and the computer can calculate the concentration of the object to be measured according to the measurement result.

The purpose of the utility model can be realized by the following technical scheme:

the utility model provides a full-automatic luminous immunoassay system of high flux, includes application of sample support, application of sample arm, application of sample needle, reagent storehouse, sample storehouse, reaction incubation dish, reaction carousel, reaction cup groove, reaction cup, the automatic subassembly of cup of adorning of reaction cup, reaction cup centre gripping arm, cleaning station subassembly, heat preservation dish, heat preservation carousel, substrate needle survey, volume darkroom subassembly and photon measuring component, install axial pivoted reaction carousel on the reaction incubation dish, the automatic subassembly of cup of adorning of reaction cup is located reaction carousel top, photon measuring component fixed mounting is in measuring darkroom subassembly one side, its characterized in that, application of sample support distributes in reaction carousel week side, and application of sample arm is installed to the application of sample support, and fixed mounting has application of sample needle on the application of sample arm.

Reaction cup clamping arms are arranged between the reaction turntable and the cleaning station component and between the cleaning station component and the measurement darkroom component.

The heat preservation plate is positioned on the inner side of the cleaning station component, the heat preservation plate is provided with a heat preservation rotating disc which rotates axially, and the substrate needle is fixedly arranged on the heat preservation plate through a substrate needle support.

Further, the reagent bin and the sample bin are respectively positioned on two sides of the reaction turntable.

Furthermore, reaction cup grooves which are distributed in a double-row axial direction are formed in the reaction rotating disc.

Further, the reaction cup clamping arm moves along the radial direction, the Z-axis direction and the rotation of the reaction turntable.

Further, the sample adding arm moves along the X-axis direction, the Z-axis direction and the rotation.

Furthermore, the sample adding arms are divided into two groups, the sample adding arm positioned on one side of the reagent bin is a reagent arm, and the sample adding arm positioned on one side of the sample bin is a sample arm.

Further, there is a time interval between the movements of the sample arms.

There is a time interval between the movements of the reagent arms.

Furthermore, the automatic reaction cup loading assembly automatically loads reaction cup slots on the inner ring and the outer ring of the reaction turntable.

Further, the test tube rack conveying system conveys the test tube racks to the positions below the corresponding sample arms, and the reagent box conveying system conveys the reagent boxes to the positions below the corresponding reagent arms.

Further, the kit delivery system places the array of kits within the reagent cartridge.

The utility model has the advantages that:

1. the utility model utilizes a plurality of sample adding arms to add samples or reagents simultaneously, thereby increasing the number of reaction cups which are added with samples or reagents in unit time and further increasing the flux of the system;

2. the utility model can set a substrate adding component outside the measurement darkroom component or outside the measurement darkroom component according to the requirement to adapt to the measurement requirement of the flash method or the glow method;

3. the utility model can delay and preserve heat of the system after adding the substrate according to the requirement under the condition of adding the substrate outside;

4. the reaction turntable of the utility model is provided with double rows of reaction cup grooves distributed axially, and the reaction cup automatic cup-loading assembly can automatically load the reaction cup grooves on the inner and outer rings of the reaction turntable, thereby improving the load of the reaction turntable on which the reaction cups are loaded;

5. the utility model discloses utilize test-tube rack conveying system to transport the below that corresponds the sample arm with the test-tube rack, kit conveying system transports the below that corresponds the reagent arm with the kit, removes kit/test-tube rack to application of sample arm below for traditional manual work, reduces artifical intensity of labour, reduces the during operation and consumes, and simultaneously, the kit in the reagent storehouse passes through conveying mechanism and realizes the kit and arrange in the reagent storehouse, further reduces artifical intensity of labour.

Drawings

The present invention will be further described with reference to the accompanying drawings.

FIG. 1 is a schematic diagram of the structure of the high-throughput fully-automatic immunofluorescence assay system of the present invention;

FIG. 2 is a schematic view of a partial structure of the present invention;

FIG. 3 is a schematic view of the structure with different viewing angles of the present invention;

FIG. 4 is a schematic view of the structure of the sample-adding arm of the present invention;

FIG. 5 is a schematic view of the structure of the delivery system of the reagent cartridge of the present invention;

FIG. 6 is a schematic view of the structure of the reagent box rotary handling mechanical arm of the present invention;

FIG. 7 is a schematic view of the reagent box carrying robot of the present invention;

fig. 8 is a schematic structural view of the test tube rack conveying system of the present invention;

fig. 9 is a schematic structural view of the test tube rack conveying system according to the present invention with different viewing angles;

FIG. 10 is a schematic view of the structure of the conveying passage of the present invention;

FIG. 11 is a perspective sectional view of the transportation path of the present invention;

FIG. 12 is a schematic view of the structure of the holding arm of the reaction cup of the present invention;

FIG. 13 is a schematic view of a partial structure of a holding arm of the reaction cup of the present invention;

fig. 14 is a partial structural schematic view of the present invention;

fig. 15 is an enlarged schematic view of the position a of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "open hole", "upper", "lower", "thickness", "top", "middle", "length", "inner", "around", and the like, indicate positional or positional relationships, are merely for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the components or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

As shown in FIG. 1, FIG. 2, FIG. 14 and FIG. 15, a high-throughput full-automatic immune luminescence analysis system comprises a system support 1, a system bottom plate 01, a sample adding support 2, a sample adding arm 3, a sample adding needle 4, a reagent bin 5, a sample bin 6, a reaction incubation tray 7, a reaction rotating tray 8, a reaction cup groove 9, a reaction cup 10, a reaction cup automatic cup loading assembly 11, a reaction cup holding arm 12, a cleaning station assembly 13, a heat preservation tray 14, a heat preservation rotating tray 15, a substrate needle 16, a measurement dark room assembly 17 and a photon measuring assembly 18.

The system bottom plate 01 is fixedly arranged on the system bracket 1.

The sample adding bracket 2, the reaction incubation disc 7, the cleaning station assembly 13 and the measurement darkroom assembly 17 are all fixedly arranged on the system bottom plate 01.

Reagent storehouse 5, sample storehouse 6 are located 8 both sides of reaction carousel respectively to reagent storehouse 5, the equal fixed mounting in sample storehouse 6 are on system support 1, place the kit that arranges the distribution in reagent storehouse 5, place the test-tube rack that arranges the distribution in sample storehouse 6.

The automatic reaction cup loading assembly 11 is positioned above the reaction turntable 8 and is fixedly arranged on the system bracket 1.

Reaction cup clamping arms 12 are fixedly arranged on the system bottom plate 01 between the reaction rotary disc 8 and the cleaning station assembly 13 and between the cleaning station assembly 13 and the measurement darkroom assembly 17.

As shown in FIG. 3, the reaction incubation tray 7 is provided with a reaction turntable 8 which axially rotates, the reaction turntable 8 is provided with two rows of reaction cup slots 9 which are axially distributed, so that the reaction turntable 8 can load more reaction cups 10 than the original single row of reaction cup slots 9 at one time, the reaction cup slots 9 are partially arranged in the reaction incubation tray 7, the reaction cups 10 are arranged in the reaction cup slots 9, the reaction cups 10 arranged in the reaction cup slots 9 are kept at a constant temperature for incubation, and the upper end of the reaction incubation tray 7 is covered with a heat preservation cover.

The photon measuring assembly 18 is fixedly mounted within one side of the measurement dark room assembly 17.

The application of sample support 2 distributes in 8 week sides of reaction carousel, and application of sample arm 3 is installed to application of sample support 2 (or 2 upper ends of application of sample support of homonymy link together, form an application of sample arm guide rail, install two application of sample arms 3 on the application of sample arm guide rail).

As shown in fig. 4, the sample-adding arm 3 includes a sample-adding X-axis support seat 31, a sample-adding Z-axis support seat 32, a sample-adding motor 33 and a sample-adding needle support 34, the sample-adding Z-axis support seat 32 is installed on the sample-adding X-axis support seat 31, the sample-adding motor 33 is fixedly installed on the upper end of the sample-adding Z-axis support seat 32, and the sample-adding needle support 34 is fixedly installed on the rotation shaft of the output end of.

Sample adding support 2 drives sample adding X shaft supporting seat 31 to move along the X shaft direction through sample adding X shaft synchronous belt 35, sample adding X shaft supporting seat 31 drives sample adding Z shaft supporting seat 32 to move along the Z shaft direction through sample adding Z shaft synchronous belt 36, wherein sample adding X shaft synchronous belt is fixedly installed on sample adding support 2, and sample adding Z shaft synchronous belt 36 is fixedly installed on sample adding X shaft supporting seat 31.

The sample adding needle support 34 is fixedly provided with a sample adding needle 4, and the sample adding needle 4 is connected with a sample adding pump through a pipeline.

During the use, the removal of X axle direction, Z axle direction is realized through X axle hold-in range 35, application of sample Z axle hold-in range 36 to application of sample needle 4, and application of sample needle 4 realizes rotating around application of sample motor 33 output pivot through application of sample motor 33.

The sample adding arms 3 are divided into two groups, the sample adding arm 3 positioned at one side of the reagent bin 5 is a reagent arm, the sample adding arm 3 positioned at one side of the sample bin 6 is a sample arm, when measuring, the transparent reaction cup 10 is placed in the reaction turntable 8, the reaction turntable 8 rotates, when the reaction turntable 8 drives the reaction cup 10 to rotate to the vicinity of the reagent arm, the single reagent arm adds the reagent in the reagent bin 5 into the reaction cup 10 through the X-axis direction, the Z-axis direction, the rotation motion of the sample adding needle 4 of the sample adding bracket 2 and the sample adding pump and the sample adding needle 4, after the reagent in the reaction cup 10 is added, the reaction turntable 8 drives the reaction cup 10 to rotate to the position near the sample arm, the single sample arm adds the sample in the sample bin 6 into the same reaction cup 10 through the X-axis direction, the Z-axis direction and the rotary motion of the sample adding arm 3 of the sample adding bracket 2, the sample adding pump and the sample adding needle 4.

The above-mentioned order of adding reagent and sample to the cuvette 10 is not limited to adding reagent first and then adding sample, but may also be adding reagent first by driving the cuvette 10 to rotate to the vicinity of the sample arm through the reaction turntable 8, and then driving the cuvette 10 to rotate to the vicinity of the sample arm through the reaction turntable 8.

The motion relationship between the sample arms and the motion relationship between the reagent arms are performed asynchronously with a time interval therebetween.

The two sample arms and the two reagent arms are used for adding samples and reagents to the reaction cups 10 on the reaction turntable 8, the samples and the reagents are added to the two reaction cups 10 at the same time, and compared with the traditional method that the samples and the reagents are added to a single reaction cup 10 by a single sample arm and a single reagent arm at the same time, the number of the reaction cups 10 added with the samples or the reagents is doubled in unit time, so that the flux of the system is improved.

Sample adding needle 4 carries out the application of sample through X axle direction, Z axle direction and rotary motion, and for traditional sample adding needle 4 carries out the application of sample through X axle direction, Y axle direction and Z direction motion, reduce application of sample in-process sample adding needle 4 from reagent or sample add to the stroke of reaction cup 10, further heighten reagent or sample adding speed in reaction cup 10.

As shown in fig. 1 and 5, the front end of the reagent chamber 5 is provided with a reagent box inlet 51, the rear end of the reagent chamber 5 is provided with a reagent box outlet 52, reagent box chutes 53 are fixedly mounted on the inner sides of the reagent box inlet 51 and the reagent box outlet 52, transfer chutes 54 are mounted between the reagent box chutes 53, the transfer chutes 54 are fixedly mounted on the reagent chamber 5, wherein the reagent box inlet 51, the reagent box chute 53, the transfer chutes 54 and the reagent box outlet 52 are kept on a straight line, and a reagent box conveying system 55 is mounted in the reagent chamber 5.

The reagent box conveying system 55 comprises a reagent box pushing plate 551, a reagent box rotary conveying mechanical arm 552 and a reagent box conveying mechanical arm 553, wherein a reagent box is manually placed at a reagent box inlet 51, the reagent box pushing plate 551 pushes the reagent box to slide to one side of the reagent box rotary conveying mechanical arm 552 along a reagent box sliding groove 53 by pushing a synchronous belt 554, the reagent box rotary conveying mechanical arm 552 lifts the reagent box, the reagent box rotary conveying mechanical arm 552 conveys the lifted reagent box through a conveying synchronous belt 555, the reagent box rotary conveying mechanical arm is conveyed to a target position of a reagent cabin 5 and is arranged, and the reagent box conveying mechanical arm 553 is positioned at one side of the reagent box rotary conveying mechanical.

When reagents in the reagent kit need to be sucked, the reagent kit rotary conveying mechanical arm 552 conveys a target reagent kit in the reagent bin 5 to the lower part of the sample adding arm 3 through the conveying synchronous belt 555, a sample adding needle 4 on the sample adding arm 3 extracts the reagents in the target reagent kit, and an empty reagent kit is conveyed to the initial position of the reagent bin 5 through the reagent kit rotary conveying mechanical arm 552 again.

After all the reagents in the reagent boxes in the reagent box 5 are extracted, the reagent box carrying mechanical arm 553 lifts the empty reagent box at the side of the reagent box outlet 52 of the reagent box 5, and the reagent box carrying mechanical arm 553 carries the lifted reagent box to the reagent box chute 53 at the side of the reagent box outlet 52 through the carrying synchronous belt 555; the reagent box rotary carrying mechanical arm 552 lifts up the empty reagent box on one side of the reagent box inlet 51 of the reagent box 5, the reagent box rotary carrying mechanical arm 552 carries the lifted reagent box to the reagent box sliding groove 53 on one side of the reagent box inlet 51 through the carrying synchronous belt 555, the reagent box push plate 551 pushes the empty reagent box to slide to the reagent box outlet 52 along the reagent box sliding groove 53 or the transmission sliding groove 54 through the push synchronous belt 554, and the empty reagent box is pushed out from the reagent box outlet 52 to finish the collection.

As shown in fig. 6, the reagent cartridge rotary carrying mechanical arm 552 includes a motor mount 5521, a lifting support 5522, a reagent cartridge clamping piece 5523, a reagent cartridge rotating pulley 5524 and a lifting guide 5525, wherein the motor mount 5521 is fixedly connected with a carrying synchronous belt 555, the lifting support 5522 is installed on the motor mount 5521 and is connected in a sliding manner, the reagent cartridge clamping piece 5523 is fixedly installed on the lifting support 5522, the reagent cartridge rotating pulley 5524 axially rotates is installed at the upper end of the motor mount 5521, the lifting guide 5525 is installed on the reagent cartridge rotating pulley 5524 and is connected in a sliding manner along the Z-axis direction, and the lifting guide 5525 is fixedly connected with the lower end of the lifting support 5522.

The motor mount 5521 drives the lifting support portion 5522 to move along the Z-axis direction through arranging the Z-axis synchronous belt 5526, the motor mount 5521 drives the reagent cartridge rotating belt wheel 5524 to rotate through arranging the rotating synchronous belt 5527, and the reagent cartridge rotating belt wheel 5524 drives the lifting support portion 5522 to rotate through the lifting guide rail 5525.

During the use, the bolt portion of kit is held up through the motion of Z axle direction to kit clip piece 5523, when needs utilize rotatory transport arm 552 of kit to carry the kit to arranging in the reagent storehouse 5 that is located reagent box export 52 one side, and lift supporting part 5522 rotates, drives the kit that holds up and rotates and arrange in the reagent storehouse 5 that is located reagent box export 52 one side.

As shown in fig. 7, the reagent cartridge transporting robot 553 includes a transporting motor mount 5531, a transporting lifting support 5532, and a transporting reagent cartridge gripping piece 5533, wherein the transporting motor mount 5531 is fixedly connected to the transporting synchronous belt 555, the transporting lifting support 5532 is slidably connected to the transporting motor mount 5531, the transporting motor mount 5531 drives the transporting lifting support 5532 to move along the Z-axis direction by moving away from the Z-axis synchronous belt 5534, the transporting reagent cartridge gripping piece 5533 is fixedly mounted on the transporting lifting support 5532, and in use, the transporting reagent cartridge gripping piece 5533 lifts up and carries the latch portion of the reagent cartridge by moving along the Z-axis direction.

As shown in fig. 8 and 9, a test tube rack conveying system 61 is installed in the sample bin 6, the test tube rack conveying system 61 includes a test tube rack Y-axis supporting seat 611, a test tube rack Z-axis supporting seat 612, a test tube rack rotating supporting seat 613, a test tube rack supporting seat 614, a test tube rack motor 615, a test tube rack lifting part 616 and a test tube rack clamping part 617, the test tube rack Y-axis supporting seat 611 is installed on the sample bin 6, the test tube rack Z-axis supporting seat 612 is installed on the test tube rack Y-axis supporting seat 611 in a sliding connection manner, the test tube rack rotating supporting seat 613 is fixedly installed at the upper end of the test tube rack Z-axis supporting seat 612, the test tube rack supporting seat 614 is installed on the test tube rack rotating supporting seat 613 in a sliding connection manner along the Z-axis direction, the test tube rack motor 615 is fixedly installed on the test tube rack supporting seat 614, an outer gear 6151 is fixedly installed, a rack lifting part 616 is fixedly mounted at the top end of the rack 6152 outside the rack, and a rack clamping part 617 is fixedly mounted on the rack lifting part 616.

Wherein, test-tube rack Y axle supporting seat 611 passes through test-tube rack Y axle hold-in range 618 and drives test-tube rack Z axle supporting seat 612 and remove on along test-tube rack Y axle supporting seat 611, realizes that test-tube rack holds up portion 616 and moves along Y axle direction, and test-tube rack Y axle hold-in range 618 fixed mounting is on test-tube rack Y axle supporting seat 611.

A test tube rack Z-axis synchronous belt 619 is fixedly installed on the test tube rack Z-axis supporting seat 612, a test tube rack lifting supporting seat 6191 is fixedly installed on the test tube rack Z-axis synchronous belt 619, and the test tube rack lifting supporting seat 6191 is rotatably connected with the lower end of the test tube rack supporting seat 614; the test tube rack supporting seat 614 is driven to move along the Z-axis direction through the test tube rack Z-axis synchronous belt 619, and the test tube rack lifting part 616 is further driven to move along the Z-axis direction.

The test-tube rack rotating synchronous belt 620 is installed at the upper end of the test-tube rack rotating supporting seat 613, the test-tube rack rotating synchronous belt 620 is internally meshed with the test-tube rack rotating seat 621, the test-tube rack rotating supporting seat 613 drives the test-tube rack supporting seat 614 to rotate around the test-tube rack rotating synchronous belt 620 and the test-tube rack rotating seat 621, and the rotation of the test-tube rack supporting part 616 is achieved.

When the device is used, the test tube rack lifting part 616 moves to the bottom of a target test tube rack in the sample bin 6 through the movement in the Y-axis direction and the movement in the Z-axis direction, the test tube rack motor 615 is started, the test tube rack outer gear 6151 on the drive shaft of the test tube rack motor 615 rotates to drive the test tube rack outer rack 6152 to move, and the test tube rack outer rack 6152 drives the test tube rack lifting part 616 to be inserted into the bottom of the target test tube rack to lift the bottom of the target test tube rack; in this process, the test-tube rack clamping portion 617 clamps the sample tube on the target test-tube rack, it is not hard up to take place between the target test-tube rack handling process and the test-tube rack conveying system 61, the test-tube rack holding portion 616 carries the target test-tube rack to the sample arm below through the movement of the Y axis direction and the movement of the Z axis direction again, wherein, the test-tube rack holding portion 616 realizes processing the test-tube rack on the other side of the sample bin 6 through self rotation, after the sample in the test-tube rack is completely absorbed by the sample adding needle, the test-tube rack conveying system 61 carries the test-tube rack to restore the initial position in the sample bin 6.

All contain conveying mechanism in reagent storehouse 5 and the sample storehouse 6, constantly carry the reagent box in reagent storehouse 5 or the test-tube rack in sample storehouse 6 to application of sample arm 3 below, move reagent box/test-tube rack to application of sample arm 3 below for traditional manual work, reduce artifical intensity of labour, reduce the during operation consumption simultaneously, the reagent box in reagent storehouse 5 realizes that the reagent box is automatic to be arranged in reagent storehouse 5 through conveying mechanism simultaneously, and further, reduces artifical intensity of labour.

As shown in fig. 1, the automatic cuvette assembly 11 includes a cuvette arranger 111 (a large capacity full automatic cuvette arranger disclosed in patent No. CN 201311274346.6), a slide 112 and a conveying channel 113, the casing of the cuvette arranger 111 is fixedly mounted on the system frame 1, and can continuously load cuvettes 10 into the reaction turntable 8 through the cuvette arranger 111, the slide 112 and the conveying channel 113, and the cuvette arranger 111 makes cuvettes 10 in the hopper fall into the slide 112 in a determined direction and fall into the cup slots of the cuvettes 10 through the conveying channel 113.

As shown in fig. 11 and 12, the conveying channel 113 includes a channel body 1131, a limiting electromagnet 1132, a driving motor 1133, a barrier 1134, an outer ring channel 1135, an inner ring channel 1136 and a reflective photoelectric switch 1137, the upper end of the channel body 1131 is communicated with the chute 112, the reaction cup 10 in the chute 112 falls into the channel body 1131, a pair of limiting electromagnets 1132 is installed outside the channel body 1131, the limiting electromagnet 1132 is activated, the output end of the limiting electromagnet 1132 located at the lower end abuts against the reaction cup 10 in the channel body 1131, the output end of the limiting electromagnet 1132 located at the upper end presses the reaction cup 10 in the channel body 1131 to limit the reaction cup 10, the driving motor 1133 is installed outside the channel body 1131, the barrier 1134 is fixedly installed on the driving shaft of the driving motor 1133, the outer ring channel 1135 and the inner ring channel 1136 are fixedly installed at the lower end of the channel body 1131, wherein the lower end outlets of the outer ring channel 1135 and the inner ring channel 1136 are, the upper end and the lower end of the channel body 1131 are fixedly provided with reflective photoelectric switches 1137.

A reaction cup limiting cover 1138 is fixedly arranged on the barrier 1134.

During the use, start spacing electro-magnet 1132, spacing electro-magnet 1132 output drive shaft is spacing to the reaction cup 10 in the passageway body 1131, single reaction cup 10 falls down to shelves strip 1134 because self gravity in the passageway body 1131 that is located shelves strip 1134 top, along shelves strip 1134 landing to outer lane passageway 1135 or inner circle passageway 1136 in, at this in-process, reaction cup 10 of landing is spacing on the shelves strip 1134 to reaction cup 1138, prevent reaction cup 10 self upset of reaction cup 10 landing in-process.

The driving motor 1133 is started, the driving shaft of the driving motor 1133 drives the barrier strips 1134 to swing, and the outer ring channel 1135 and the inner ring channel 1136 are selectively dropped through changing the swinging direction and the swinging angle of the barrier strips 1134, so that the reaction cups 10 are automatically filled in the reaction cup grooves 9 on the outer ring or the inner ring of the reaction turntable 8.

In the process, the reflective photoelectric switch 1137 located at the lower end of the channel body 1131 monitors the reaction cup 10 at the top end of the barrier 1134, and when the reaction cup 10 is located at the top end of the barrier 1134, the barrier 1134 swings normally; when no reaction cup 10 is arranged at the top end of the barrier 1134, the reaction cup 10 at the upper end of the barrier 1134 falls into the outer ring channel 1135 or the inner ring channel 1136, at this time, the driving shaft at the output end of the limit electromagnet 1132 contracts to perform the next cup falling on the reaction cup 10 in the channel body 1131, and the reaction cup 10 is inversely sleeved on the barrier 1134, at this time, the driving motor 1133 drives the upper end of the barrier 1134 to rotate to the vicinity of the outer ring channel 1135 or the inner ring channel 1136, and in this process, the reaction cup 10 at the top end sleeve of the barrier 1134 slides into the outer ring channel 1135 or the inner ring channel 1136 due to its own gravity.

The reflective photoelectric switch 1137 located at the upper end of the channel body 1131 monitors the reaction cups 10 in the upper end of the channel body 1131, and when the reflective photoelectric switch 1137 monitors that the reaction cups 10 exist at the upper end of the channel body 1131, this indicates that the reaction cups 10 in the channel body 1131 are fully accumulated, and at this time, the reaction cup arrangement device 111 is controlled to stop operating, so as to avoid a great amount of accumulation of the reaction cups 10 in the channel body 1131 and the slide track 112; when the cuvette 10 is not detected by the reflective photoelectric switch 1137 at the upper end of the channel body 1131, the cuvette alignment apparatus 111 is normally operated to supply the cuvette 10 to the channel body 1131 through the slide channel 112.

As shown in fig. 12, the cuvette holding arm 12 includes a radial support seat 121, a synchronous pulley support seat 122, a holding synchronous pulley 123, a holding guide 124 and a holding portion 125, the radial support seat 121 is installed on the system bottom plate 01, the synchronous pulley support seat 122 is installed on the radial support seat 121, the holding synchronous pulley 123 rotating around the axis is installed on the upper end of the synchronous pulley support seat 122, the holding guide 124 slidably connected along the Z-axis direction is installed on the holding synchronous pulley 123, and the holding portion 125 is fixedly installed on the upper end of the holding guide 124.

Radial hold-in range 126 is fixed mounting on system bottom plate 01, and radial supporting seat 121 fixed mounting is on radial hold-in range 126, and system bottom plate 01 passes through radial hold-in range 126 and drives radial supporting seat 121 along 01 linear motion of system bottom plate.

Radial supporting seat 121 is provided with clamping Z-axis synchronous belt 127, clamping Z-axis supporting seat 120 is fixedly mounted on clamping Z-axis synchronous belt 127, the lower end of clamping guide rail 124 is rotatably connected with clamping Z-axis supporting seat 120, and radial supporting seat 121 drives clamping Z-axis supporting seat 120 to move along the Z-axis direction through clamping Z-axis synchronous belt 127.

The synchronous pulley supporting seat 122 is fixedly provided with a clamping rotary synchronous belt 128, the clamping synchronous pulley 123 is meshed and connected to the inner side of the clamping rotary synchronous belt 128, the synchronous pulley supporting seat 122 drives the clamping synchronous pulley 123 to rotate through the clamping rotary synchronous belt 128, and the clamping synchronous pulley 123 drives the clamping part 125 to rotate through the clamping guide rail 124.

In use, the clamping portion 125 performs linear motion along the radial direction and the Z-axis direction of the reaction turntable 8 and rotational motion around the axis of the clamping timing pulley 123 through the radial timing belt 126, the clamping Z-axis timing belt 127 and the clamping rotary timing belt 128.

As shown in fig. 13, the clamping portion 125 includes a clamping support portion 1251, a clamping housing 1252, an extensible electromagnet 1253, an upper rack 1254, a lower rack 1255, an outer gear 1256 and a clamping strip 1257, the clamping support portion 1251 is fixedly connected to the top end of the guide rail 124, the clamping housing 1252 is fixedly mounted on the clamping support portion 1251, the extensible electromagnet 1253 is fixedly mounted on the clamping support portion 1251, the upper rack 1254 is fixedly mounted on the top end of an extensible rod of the extensible electromagnet 1253, the upper rack 1254 is located at the upper end inside the clamping housing 1252, the lower rack 1255 is slidably connected to the lower end inside the clamping housing 1252, the upper rack 1254 and the lower rack 1255 are mounted between the outer gear 1256, the upper rack 1254 and the lower rack 1255 are both engaged with the outer gear 1256, and the clamping strip 1257 is fixedly mounted on the lower ends of the upper rack 1254.

The lower end of the clamping bar 1257 extends outside the clamping housing 1252.

During the use, start flexible electro-magnet 1253, flexible end drive upper rack 1254 of flexible electro-magnet 1253 removes, and upper rack 1254 removes through the motion transmission, drives lower rack 1255 and removes, realizes that centre gripping strip 1257 keeps away from each other or presss from both sides tightly, carries out centre gripping or release to placing reaction cup 10 between centre gripping strip 1257.

As shown in fig. 14, the cleaning station assembly 13 includes a magnetic separating base 131, a cleaning turntable 132, a suction needle 133 and a cleaning needle 134 (where the magnetic separating base 131 and the cleaning turntable 132 are respectively a reaction cup for a luminometer of patent application No. cn201410856244.x and a magnetic separating base and a cleaning turntable disclosed in a corresponding cleaning and separating mechanism thereof), the cleaning turntable 132 rotating around an axis is installed on the magnetic separating base 131, the cleaning turntable 132 is provided with axially distributed reaction cup slots 9, the magnetic separating base 131 is fixedly installed with the suction needle holder 135, the suction needle holder 135 is installed with a suction needle supporting plate 136 moving along a Z-axis direction, the suction needle 133 is fixedly installed on the suction needle supporting plate 136, the cleaning needle 134 is installed on the magnetic separating base 131 directly below the suction needle 133, and the cleaning needle 134 is fixedly installed on the magnetic separating base 131 through the cleaning needle holder 137.

The reaction cup holding arm 12 is located between the reaction turntable 8 and the magnetic separation seat 131 and between the magnetic separation seat 131 and the measurement dark room assembly 17.

When the incubation time of a certain cuvette 10 reaches a predetermined time, the reaction rotor 8 is rotated to a predetermined position and moved to the cuvette well 9 of the washing rotor 132 by the cuvette holding arm 12.

After the reaction cup 10 with reactants is placed in the reaction cup slot 9 of the cleaning turntable 132, the cleaning turntable 132 rotates according to a certain time period, and reaches the lower part of the suction needle 133 and the washing needle 134 through the separation magnet in the magnetic separation seat 131 in sequence, the suction needle 133 firstly pumps away the liquid in the reaction cup 10 through the suction needle pump, the washing needle 134 injects the washing liquid into the reaction cup 10 through the washing needle pump, then the cleaning turntable 132 continuously rotates according to a certain time period, when the reaction cup 10 reaches the last suction needle 133, the magnetic beads move from one side of the cup to the other side for 4 times, and the reaction cup 10 realizes 4 times of cleaning, so that the reliability and the repeatability of the measurement result are ensured, the cleaning times are reduced, and the testing speed is accelerated.

The heat preservation plate 14 is fixedly installed at the upper end of the system bottom plate 01, the heat preservation plate 14 is located on the inner side of the cleaning station assembly 13, as shown in fig. 15, an axially-rotating heat preservation turntable 15 is installed on the heat preservation plate 14, axially-distributed reaction cup grooves 9 are formed in the heat preservation turntable 15, and the heat preservation plate 14 preserves heat of reaction cups 10 in the reaction cup grooves 9 on the heat preservation turntable 15.

The substrate pins 16 are fixedly mounted to the thermal plate 14 by a substrate pin holder 161.

After the reaction cup 10 is cleaned, if the reaction cup is cleaned by the one-step method, after the cleaning is finished, the reaction cup 10 is moved to the reaction cup groove 9 of the heat-preservation rotating disc 15 by the reaction cup clamping arm 12, the substrate is selectively added to the reaction cup 10 by the substrate needle 16 by the substrate pump, and the heat-preservation rotating disc 15 is rotated until the reaction cup 10 is positioned below the reaction cup clamping arm 12 beside the measurement darkroom assembly 17.

For some reagents, the two-step or three-step method, after the washing, the cuvette holding arm 12 does not move the cuvette 10 to the cuvette slot 9 of the thermal insulation rotary disk 15, but returns the cuvette 10 to the reaction rotary disk 8, and then the sample loading and incubation processes of the reagent or the sample are performed, and the procedure is the same as that of the one-step method.

When the reaction cup 10 on the heat-insulating rotary disk 15 rotates to the position below the reaction cup holding arm 12 beside the measurement darkroom assembly 17, the reaction cup 10 is moved to the drawer cup groove of the measurement darkroom assembly 17 (such as the measurement darkroom assembly disclosed in a full-automatic biochemical and luminescence immunoassay system of patent number CN 201310312544.7) through the reaction cup holding arm 12, the drawer is closed, the measurement darkroom assembly 17 is sealed, the light shielding plate in the measurement darkroom assembly 17 is opened, the reaction cup 10 is positioned in front of the photoelectric detector of the photon measurement assembly 18 (such as the photon measurement assembly disclosed in the full-automatic biochemical and luminescence immunoassay system of patent number CN 201310312544.7), the photon measurement assembly 18 measures the luminescence product in the reaction cup 10, and the computer calculates the concentration of the object to be measured according to the measurement result.

When the reaction cup clamping arm 12 moves the reaction cup 10 to the reaction cup groove 9 of the heat preservation turntable 15, if the measurement requirement of the flash method is met, the heat preservation turntable 15 is rapidly rotated until the reaction cup 10 is positioned below the reaction cup clamping arm 12 beside the measurement darkroom assembly 17, the reaction cup clamping arm 12 moves the reaction cup 10 to the measurement darkroom assembly 17, the substrate in the darkroom assembly 17 is added into the reaction cup 10, the photon measurement assembly 18 measures the luminous product in the reaction cup 10, and the computer calculates the concentration of the object to be measured according to the measurement result.

If the measurement requirement of the glow method is required, the rotating speed of the heat preservation rotating disc 15 is controlled according to the requirement to delay and preserve the temperature of the reaction cup 10, and the adding of the substrate to the reaction cup 10 is not limited to the adding of the substrate to the reaction cup 10 through the substrate needle 16 positioned on the heat preservation disc 14, and the substrate can also be added to the reaction cup 10 through the substrate needle in the measurement darkroom assembly 17.

After the measurement, the drawer is opened, and by reaction cup centre gripping arm 12 with reaction cup 10 remove to the useless cup groove in, the waste liquid pump is taken away the waste liquid through waste liquid needle group, and waste reaction cup 10 falls into waste reaction cup passageway to draw forth the outside of instrument.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention.

Claims

1. A high-flux full-automatic immune luminescence analysis system comprises a sample adding bracket (2), a sample adding arm (3), a sample adding needle (4), a reagent bin (5), a sample bin (6), a reaction incubation disc (7), a reaction turntable (8), a reaction cup groove (9), a reaction cup (10), a reaction cup automatic cup loading assembly (11), a reaction cup clamping arm (12), a cleaning station assembly (13), an insulation disc (14), an insulation turntable (15), a substrate needle (16), a measurement darkroom assembly (17) and a photon measurement assembly (18), wherein the reaction incubation disc (7) is provided with the reaction turntable (8) which axially rotates, the reaction cup automatic cup loading assembly (11) is positioned above the reaction turntable (8), the photon measurement assembly (18) is fixedly arranged in one side of the measurement darkroom assembly (17), and is characterized in that the sample adding bracket (2) is distributed on the reaction turntable (8), the sample adding support (2) is provided with a sample adding arm (3), and a sample adding needle (4) is fixedly arranged on the sample adding arm (3);

reaction cup clamping arms (12) are arranged between the reaction turntable (8) and the cleaning station assembly (13) and between the cleaning station assembly (13) and the measurement darkroom assembly (17);

the heat preservation plate (14) is positioned on the inner side of the cleaning station component (13), the heat preservation plate (14) is provided with a heat preservation turntable (15) which rotates axially, and the substrate needle (16) is fixedly arranged on the heat preservation plate (14) through a substrate needle bracket (161).

2. The high-throughput full-automatic immune luminescence analysis system according to claim 1, characterized in that the reagent chamber (5) and the sample chamber (6) are respectively located at two sides of the reaction turntable (8).

3. The high-throughput full-automatic immune luminescence analysis system according to claim 1, wherein said reaction turntable (8) is provided with two rows of axially distributed reaction cup slots (9).

4. The high throughput fully automated immunofluorescence analysis system according to claim 1, wherein, the reaction cup holder arm (12) moves along the reaction carousel (8) in radial, Z-axis direction and rotation.

5. The high throughput fully automatic immunofluorescence assay system according to claim 1, wherein, the sample application arm (3) moves along X-axis direction, Z-axis direction and rotation.

6. The high-throughput full-automatic immunofluorescence analysis system according to claim 2, wherein, the sample adding arms (3) are divided into two groups, the sample adding arm (3) located at one side of the reagent bin (5) is a reagent arm, and the sample adding arm (3) located at one side of the sample bin (6) is a sample arm.

7. A high throughput fully automated immunoradiometric assay system according to claim 6, wherein there is a time interval between the movements of the sample arms;

there is a time interval between the movements of the reagent arms.

8. The high-throughput full-automatic immune luminescence analysis system according to claim 1, wherein said reaction cup automatic cup-loading assembly (11) automatically cups the reaction cup slots (9) on the inner and outer circles of the reaction turntable (8).

9. The high-throughput full-automatic immune luminescence analysis system according to claim 6, characterized in that a test tube rack conveying system (61) is installed in the sample bin (6), the test tube rack conveying system (61) conveys the test tube rack below the corresponding sample arm, and the reagent box conveying system (55) conveys the reagent box below the corresponding reagent arm.

10. A high throughput fully automated immunofluorescence analysis system according to claim 9, wherein, the kit delivery system (55) places the kit array in the reagent cartridge bay (5).