CN109142708B - Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance - Google Patents

Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance Download PDF

Info

Publication number
CN109142708B
CN109142708B CN201811016234.XA CN201811016234A CN109142708B CN 109142708 B CN109142708 B CN 109142708B CN 201811016234 A CN201811016234 A CN 201811016234A CN 109142708 B CN109142708 B CN 109142708B
Authority
CN
China
Prior art keywords
assembly
axis
plate
tray
reading
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201811016234.XA
Other languages
Chinese (zh)
Other versions
CN109142708A (en
Inventor
李根平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Biokey Health Technology Co ltd
Original Assignee
Guangzhou Biokey Health Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Biokey Health Technology Co ltd filed Critical Guangzhou Biokey Health Technology Co ltd
Priority to CN201811016234.XA priority Critical patent/CN109142708B/en
Publication of CN109142708A publication Critical patent/CN109142708A/en
Priority to PCT/CN2019/078574 priority patent/WO2020042596A1/en
Application granted granted Critical
Publication of CN109142708B publication Critical patent/CN109142708B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations

Abstract

The invention discloses a full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer, wherein a rack divides the analyzer into an inner chamber and an outer chamber; an electronic component box is fixed on the upper layer of the inner chamber, a darkroom shell is positioned on the lower layer of the inner chamber, and a darkroom door device is arranged on the rack and matched with the darkroom shell to form a darkroom space with one side capable of being opened and closed; the tray module is positioned at the lower layer of the outer chamber, can be used for placing a plurality of reagent strips and sending the reagent strips to the lower layer of the inner chamber or returning the reagent strips to the lower layer of the outer chamber; the puncture liquid-transferring module is positioned on the upper layer of the outer chamber, can automatically puncture the thin film on the top surface of the reagent strip and automatically suck or discharge liquid in the reagent strip; the code scanning reading module is positioned on the lower layer of the inner chamber and inside the darkroom shell and is used for exciting and reading the fluorescence information of the incubated reagent strip; through the reasonable layout of interior outer room, combine the compact scientific design to relevant function module, not only the structure is simple more compact, the cost is cheaper, has improved the holistic degree of automation of instrument moreover, and the medical unit that is fit for china very much uses, promotes and popularizes.

Description

Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance
Technical Field
The invention relates to the field of time-resolved fluorescence immunoassay instruments used in the industries of in-vitro diagnosis and biomedicine, animal epidemic diseases, food safety, drug screening and the like, in particular to a full-automatic magnetic bead time-resolved fluorescence immunoassay instrument with simple and compact structure and high automation degree.
Background
POCT Point-of-Care Testing (Point of Care Testing) is an important development direction of the in vitro diagnosis industry marker immunization technology in recent years and is also the fastest growing branch. POCT involves a variety of technologies, and in general, can be classified into simple color development, enzyme labeling, immunoassay, optical and electrical biosensors, electrochemical detection, spectrophotometry, biochips, and the like.
The labeled immunity technology has been developed for hundreds of years, and detection methods of the labeled immunity technology can be divided into the following methods according to different markers: 1. radioimmunoassay, immunoradiometric assay (RIA, IRMA); 2. fluorescence Immunoassay (FIA); 3. enzyme Immunoassay (EIA); 4. gold-labeled immunoassay; 5. bioluminescence Immunoassay (BIA); 6. enzyme-fluorescent immunoassay (E-CIA); 7. chemiluminescence immunoassay (CLIA); 8. time-resolved fluoroimmunoassay (TRFIA); 9. electrochemiluminescence immunoassay (ECLIA).
Time-resolved fluoroimmunoassay (TRFIA) adopts lanthanide chelates of europium, terbium, samarium and dysprosium as markers, has a wider excitation spectrum and a narrower emission spectrum, is beneficial to reducing the background and improving the sensitivity; the ultraviolet light excitation has the advantages of higher quantum yield, larger Stokes shift, avoidance of the superposition of an excitation spectrum, a fluorescence emission spectrum and a spectrum emitted by a biological matrix, long fluorescence decay time and the like, and has wider detection range and better specificity compared with the traditional fluorescent material.
The magnetic bead time-resolved fluorescence immunoassay is a time-resolved fluorescence immunoassay technology which is based on immunomagnetic beads and takes rare earth ion chelates as markers, is an immunoassay method which is high in sensitivity, specificity, stability, free of radioactive pollution, good in detection repeatability, wide in standard curve range and wide in application range, overcomes the defects of isotope pollution, short half-life period, poor stability of Enzyme Immunoassay (EIA) and the like of Radioimmunoassay (RIA), can be used for combined detection of multiple substances, is widely applied to biomedical research and clinical medical examination, and is considered to be one of trace detection means with the greatest development prospect.
The magnetic beads utilized in the magnetic bead time-resolved fluorescence immunoassay refer to immunomagnetic microspheres (IMMS) or immunomagnetic beads (IMB), and are a novel material developed by combining immunology and superparamagnetic magnetic beads. Immunomagnetic beads are superparamagnetic microspheres coated with or having a binding function for an antibody, which, when incubated in admixture with a sample containing a target substance, specifically bind to the target substance to form a magnetically responsive complex, which is retained by a magnetic field and thereby separated from other impurities in the sample. The immunomagnetic bead technology has the advantages of high separation purity, reserved target substance activity, high sensitivity, high specificity, high detection speed, low toxicity, good repeatability, simple operation, no need of expensive instruments and equipment and the like.
The superparamagnetic microspheres modified by chemical groups as a coating medium, antigen or antibody can be coupled to magnetic beads through covalent bonding, and the physical adsorption effect of the superparamagnetic microspheres is firmer than that of a microporous plate taking polystyrene as a material; the surface area of the magnetic beads is larger, more protein molecules can be combined, and the antibody loss is greatly reduced relative to plate adsorption; the immune separation and enrichment are integrated; as a small mobile phase carrier, the reaction can reach dynamic equilibrium more quickly, thereby accelerating the reaction speed; integrating a plurality of magnetic beads coupled with antibodies aiming at different antigens, so that the detection of different substances to be detected in the same sample becomes possible; the coating of a polystyrene plate (solid phase carrier) is not needed, the reagent cost is low, and the production quality is controllable.
The immune complex formed by the magnetic separation technology is directly precipitated in an external magnetic field, and the immune complex can be separated from unbound substances without centrifugation. The magnetic beads have larger binding area and can be dispersed in a liquid phase to fully react, so that the detection range is enlarged, the reaction time is shortened, and the sensitivity is greatly improved. Because the magnetic beads are coupled with the antigen or the antibody in a covalent mode, instability of physical adsorption is overcome, and therefore the immune magnetic beads are stored for a longer time and are more stable.
However, the development of the full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer in recent years in China is still in the beginning stage, and how to independently develop the full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer with simple and compact structure and high automation degree becomes one of the important research directions in the in vitro diagnosis and biomedical industries.
Disclosure of Invention
In order to solve the technical problems, the invention provides a full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer which is simpler and more compact in structure, lower in cost, high in automation degree and simple, convenient and quick to operate.
The technical scheme of the invention is as follows: a full-automatic magnetic bead time-resolved fluorescence immunoassay appearance includes: the device comprises a rack, a tray module, a puncture liquid-transferring module, a darkroom shell, a darkroom door device and a code scanning and reading module; wherein: the frame divides the whole instrument into an inner chamber and an outer chamber; an electronic component box is fixed on the upper region of the inner chamber, a darkroom shell is positioned on the lower region of the inner chamber, and a darkroom door device is arranged on the rack and is used for forming a darkroom space with one side capable of being opened and closed by matching with the darkroom shell; the tray module is positioned in the lower layer area of the outer chamber and used for placing and fixing a plurality of reagent strips to be detected and feeding the reagent strips into the lower layer area of the inner chamber or withdrawing the reagent strips to the lower layer area of the outer chamber; the puncture liquid-transferring module is positioned in the upper layer area of the outer chamber and is used for automatically puncturing a thin film which is arranged on the reagent strip and covers the top surface of the reagent tube and automatically sucking or discharging liquid in the reagent strip; the code scanning reading module is positioned in the lower layer area of the inner chamber and is positioned in the inner part of the dark room shell and used for exciting and reading the fluorescence information of the reagent strip after incubation.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the tray module consists of a tray module power mechanism and a tray module body, the tray module body is arranged on the tray module power mechanism, and the tray module power mechanism is used for feeding the tray module body which is provided with a plurality of reagent strips side by side into the lower layer area of the inner chamber or withdrawing the tray module body to the lower layer area of the outer chamber; the tray module power mechanism comprises a tray stepping motor, two tray slide rails, an encoder and a tray synchronous belt assembly; the tray synchronous belt assembly is positioned above one of the tray slide rails, the tray module body is erected on the two tray slide rails, and one side of the tray module body is connected with a synchronous belt in the tray synchronous belt assembly through a corresponding connecting piece; the tray stepping motor and the encoder are respectively positioned at two ends of the tray synchronous belt assembly, are fixed at two ends of a tray sliding rail below the tray synchronous belt assembly through respective fixing parts, and are in transmission connection with a synchronous belt in the tray synchronous belt assembly through a synchronous belt pulley in the tray synchronous belt assembly and a corresponding bearing; the encoder is used for cooperating the tray stepping motor to stop the tray module body at the position required to reach.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the tray module body comprises a tray bottom plate, a needle tube supporting block, a reagent strip tray, a reagent strip pressing sheet, a reagent strip clamping buckle, a heating sheet fixing plate, a magnet assembly power mechanism and a magnet upper and lower optocoupler assembly; the tray bottom plate is erected on the two tray slide rails, and a connecting piece for connecting a synchronous belt in the tray synchronous belt component is fixed on one side of the tray bottom plate; the needle tube supporting block and the reagent strip tray are fixed above the tray bottom plate through corresponding left and right supporting pieces; the needle tube supporting block is correspondingly provided with a plurality of rows of needle head holes for placing three needle heads, each row of needle head holes are positioned on the extension line of the rear end of the open slot corresponding to the needle head hole, the needle tube supporting block is also provided with a plurality of sample holes for placing test tubes containing samples to be detected, each sample hole is also positioned on the extension line of the rear end of the open slot corresponding to the sample hole, and the needle head holes arranged in rows are positioned between the open slot corresponding to the sample hole and the sample hole; a plurality of open slots which are matched with the reagent strips to be placed are arranged on the reagent strip tray side by side; the reagent strip pressing sheets are distributed on the top surfaces of the reagent strip trays on the two sides of the open slot; the reagent strip clip is positioned on the bottom surface of the reagent strip tray at the front end of the open slot; the heating plate is attached to the bottom surface of the heating plate fixing plate, the heating plate fixing plate is positioned at the tail part of the open slot and fixed below the reagent strip tray, and a plurality of heating lugs are arranged on the top surface of the heating plate fixing plate at intervals upwards; the magnet assembly, the magnet assembly power mechanism and the magnet upper and lower optocoupler assemblies are positioned between the tray bottom plate and the reagent strip tray, and the magnet assembly is positioned below the heating sheet fixing plate and the heating sheet; the magnet assembly power mechanism is used for lifting or lowering the magnet assembly to complete the step of separating magnetic beads from the reagent strip; and the upper and lower magnet optocoupler component is arranged between the magnet component and the magnet component power mechanism and is used for detecting the position of the magnet component in the tray module body.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the magnet assembly comprises a magnet fixing seat, a plurality of large magnets, two guide pillars and two guide pillar springs; the bottoms of the two guide posts are fixed on the tray bottom plate, the magnet fixing seat is sleeved on the two guide posts through corresponding linear bearings and can move up and down, and the two guide post springs are respectively sleeved on the two guide posts and abut against the space between the magnet fixing seat and the heating plate fixing plate and are used for assisting the magnet fixing seat to move downwards; all the large magnets are vertically arranged on the magnet fixing seat at intervals and are positioned on the extension line of the rear end of the open slot corresponding to the large magnets, and the top of each large magnet is intersected by two inclined top surfaces along the length direction of the open slot to form a conical surface; correspondingly, a magnet through hole matched with the corresponding large magnet to penetrate is formed in the heating sheet fixing plate between the two heating lugs.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the magnet assembly power mechanism comprises a motor mounting plate, a direct current brushless motor, an eccentric disc, a bearing and a connecting shaft thereof; the motor mounting plate is vertically arranged on the tray bottom plate, and the large surface of the motor mounting plate faces the magnet fixing seat; the direct current brushless motor is transversely fixed on the motor mounting plate, and an output shaft of the direct current brushless motor penetrates through the motor mounting plate and faces the magnet fixing seat; the eccentric disc is connected to an output shaft of the DC brushless motor, the bearing and a connecting shaft thereof are fixed on the disc surface of the eccentric disc, and the fixed position deviates from the output shaft of the DC brushless motor; one side of the magnet fixing seat facing the magnet assembly power mechanism is transversely provided with an adaptive bearing and a long circular hole in which a connecting shaft of the adaptive bearing rolls, and the adaptive bearing and the long circular hole are used for stirring the magnet fixing seat to drive the large magnet to ascend or descend under the driving of the direct current brushless motor.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the puncture liquid-transfering module comprises a puncture liquid-transfering stepping motor, a puncture liquid-transfering synchronous belt assembly, a puncture liquid-transfering screw transmission assembly, a liquid-transfering tube-returning assembly, a needle-returning head selection assembly, a puncture liquid-transfering guide rail slide block assembly and a large sliding plate; the liquid transferring and withdrawing assembly and the needle withdrawing head selection assembly are both arranged at the middle lower part of the front surface of the large sliding plate; the back surface of the large sliding plate is connected with a sliding block in the puncture pipetting guide rail sliding block assembly through screws, and two guide rails in the puncture pipetting guide rail sliding block assembly are vertically fixed on the front surface of the rack at intervals through screws; the puncture pipetting stepping motor is vertically arranged on the back of the rack through a corresponding fixing piece, an output shaft of the puncture pipetting stepping motor is arranged upwards and is connected with one synchronous pulley in the puncture pipetting synchronous belt assembly through a corresponding bearing; the screw rod in the puncture pipetting screw rod transmission assembly is also vertically arranged on the back of the rack through a corresponding bearing and a fixing piece, and the upper end of the screw rod is connected with another synchronous belt wheel in the puncture pipetting synchronous belt assembly through a corresponding bearing; the nut in the puncture pipetting screw rod transmission assembly is arranged on the back surface of the large sliding plate through a corresponding fixing piece and is positioned on the longitudinal central axis of the large sliding plate; the synchronous belts in the puncture liquid-transferring synchronous belt component are sleeved on the two synchronous belt wheels and are used for driving the screw rod to rotate under the driving of the puncture liquid-transferring stepping motor, and the screw rod is driven by the nut matched with the screw rod to drive the large sliding plate and the sliding block on the back of the large sliding plate to slide up and down along the guide rail, so that the liquid-transferring pipe withdrawing component and the needle withdrawing head selecting component which are installed on the front of the large sliding plate integrally move up and down.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the liquid transferring and tube withdrawing assembly comprises a liquid transferring and tube withdrawing motor fixing plate, a liquid transferring and tube withdrawing screw motor, a liquid transferring and tube withdrawing guide rail sliding block assembly, a small sliding plate, an injector push plate, a liquid transferring and tube withdrawing motor screw cap, a plurality of puncture needles, an injector fixing plate, a plurality of liquid transferring injectors and an optical coupling sensor; the pipette withdrawing motor fixing plate is transversely and vertically fixed on the front surface of the large sliding plate, the pipette withdrawing screw motor is installed on the pipette withdrawing motor fixing plate, and a screw of the pipette withdrawing screw motor penetrates through the pipette withdrawing motor fixing plate and is vertically arranged downwards; two guide rails in the liquid-transferring and tube-withdrawing guide rail sliding block assembly are vertically fixed on the front surface of a large sliding plate below a liquid-transferring and tube-withdrawing motor fixing plate at intervals through screws, the back surface of the small sliding plate is connected with a sliding block in the liquid-transferring and tube-withdrawing guide rail sliding block assembly, an injector push plate is fixed on the top of the small sliding plate, and a liquid-transferring and tube-withdrawing motor nut is installed on the injector push plate and matched with a screw rod of a liquid-transferring and tube-withdrawing screw rod motor; a plurality of puncture needles are vertically fixed on the bottom surface of the small sliding plate at intervals; the syringe fixing plate is positioned below the liquid transferring and tube withdrawing motor fixing plate and is connected with the liquid transferring and tube withdrawing motor fixing plate through two upright posts; the multiple pipetting injectors are vertically arranged on the top surface of the injector fixing plate at intervals, and the front edge of the injector push plate is provided with multiple clamping grooves which are adaptive to and clamped at the tail parts of the multiple pipetting injectors; the optical coupling sensor is connected to the front surface of a large sliding plate below the pipette withdrawing motor fixing plate through a corresponding fixing piece.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the needle withdrawing head component comprises a plurality of needle withdrawing columns, a plurality of needle withdrawing plates, a needle withdrawing head stepping motor, a needle withdrawing head synchronous belt component and a needle withdrawing head guide rail sliding block component; the lower ends of the two needle withdrawing columns are connected with a needle withdrawing plate, and the front end of the needle withdrawing plate is provided with a notch which is matched with the head needle of the pipetting injector; the needle withdrawing head stepping motor is transversely inwards connected to one side of the small sliding plate through a corresponding fixing piece, an output shaft of the needle withdrawing head stepping motor is connected with one synchronous pulley in the needle withdrawing head synchronous belt assembly through a corresponding bearing, and the other synchronous pulley in the needle withdrawing head synchronous belt assembly is connected to the other side of the small sliding plate through a corresponding bearing and a connecting shaft; the guide rail in the needle withdrawing guide rail sliding block assembly is transversely fixed at the lower end of the front surface of the small sliding plate through a screw, a sliding block in the needle withdrawing guide rail sliding block assembly is connected with a synchronous belt in the needle withdrawing synchronous belt assembly through a corresponding connecting piece, and the synchronous belt is sleeved on two synchronous belt wheels in the needle withdrawing synchronous belt assembly.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the darkroom door device comprises a darkroom door, two darkroom door guide rail sliding block assemblies and a darkroom door power mechanism; the back of the darkroom door is respectively provided with a groove and a sliding block connecting hole which are matched and embedded into the guide rail sliding block assemblies of the two darkroom doors, the darkroom door is connected to a sliding block in the guide rail sliding block assembly of the darkroom door by passing through the sliding block connecting holes through screws, and the guide rail in the guide rail sliding block assembly of the darkroom door is vertically fixed on the front of the frame through the screws; one side of the darkroom door is connected with a darkroom door power mechanism through a corresponding darkroom door connecting piece; the darkroom door power mechanism consists of a darkroom door stepping motor, the darkroom door connecting piece, a darkroom door synchronous belt component and a darkroom door power mechanism support component; the timing belt in the camera chamber door timing belt assembly is vertically positioned at one side of the camera chamber door, and the camera chamber door connecting piece is connected between the timing belt and the camera chamber door through a screw; at the upper end of the synchronous belt, the darkroom door stepping motor is fixed on the front surface of the frame through a darkroom door stepping motor support in a darkroom door power mechanism support assembly, and a synchronous pulley in the darkroom door synchronous belt assembly is connected to an output shaft of the darkroom door stepping motor through a corresponding bearing; at the lower end of the synchronous belt, a synchronous belt support in the darkroom door power mechanism support assembly is fixed on the front surface of the frame, and the other synchronous belt wheel in the darkroom door synchronous belt assembly is connected to the synchronous belt support through a corresponding bearing and a connecting shaft; the synchronous belt is sleeved on the two synchronous belt wheels.
The full-automatic magnetic bead time-resolved fluorescence immunoassay appearance, wherein: the value reading module consists of a value reading X-axis power mechanism, a value reading Y-axis power mechanism, a lens assembly and a bar code device; the reading X-axis power mechanism and the reading Y-axis power mechanism are respectively used for moving the lens component in the X-axis direction and the Y-axis direction in the horizontal plane, and the reading X-axis power mechanism is also used for moving the lens component in the X-axis direction in the horizontal plane and simultaneously moving the bar code device; the lens assembly is used for carrying out effective light-emitting data acquisition on reagent tubes which are placed on a plurality of reagent strips in the tray module and need to be read, and the bar code device is used for reading bar code information on the plurality of reagent strips; the value reading X-axis power mechanism comprises a value reading X-axis stepping motor, a value reading X-axis synchronous wheel component, a value reading X-axis guide rail sliding block component and a value reading X-axis fixing plate; the reading X-axis fixing plate is erected on a bottom plate of the rack through two upright posts; the X-axis stepping motor is installed on the front face of a vertical plate of the rack along the Y-axis direction through a corresponding fixing piece, an output shaft of the X-axis stepping motor penetrates through the vertical plate and is connected with one synchronous belt wheel in the value reading X-axis synchronous wheel assembly through a corresponding bearing, and the other synchronous belt wheel in the value reading X-axis synchronous wheel assembly is connected to the back face of the vertical plate of the rack through a corresponding bearing, a connecting shaft and a fixing piece; the guide rail in the reading X-axis guide rail sliding block assembly is fixed on a reading X-axis fixing plate between the two synchronous belt wheels along the X-axis direction through a screw, the bottom surface of one end of a reading Y-axis fixing plate in the reading Y-axis power mechanism is connected with a sliding block in the reading X-axis guide rail sliding block assembly, and the top surface of one end of the reading Y-axis fixing plate is connected with a synchronous belt in the reading X-axis synchronous wheel assembly through a corresponding connecting piece; the reading Y-axis power mechanism comprises a reading Y-axis stepping motor, a reading Y-axis synchronizing wheel assembly, a reading Y-axis guide rail sliding block assembly, the reading Y-axis fixing plate, a reading Y-axis roller and a reading Y-axis supporting plate; the reading Y-axis support plate is also erected on a bottom plate of the rack through two upright posts, and the reading Y-axis roller is installed at the other end of the reading Y-axis fixing plate; the Y-axis stepping motor is arranged at the roller end of the value reading Y-axis fixing plate along the X-axis direction through a corresponding fixing piece, the output shaft of the Y-axis stepping motor is connected with one synchronous belt wheel in the value reading Y-axis synchronous wheel component through a corresponding bearing, and the other synchronous belt wheel in the value reading Y-axis synchronous wheel component is fixed at the other end of the value reading Y-axis fixing plate through a corresponding bearing, a connecting shaft and a fixing piece; a guide rail in the reading Y-axis guide rail sliding block component is fixed on a reading Y-axis fixing plate between the two synchronous belt wheels along the Y-axis direction through a screw, the lens component is connected with a sliding block in the reading Y-axis guide rail sliding block component, and the lens component is connected with a synchronous belt in the reading Y-axis synchronous wheel component; the lens assembly comprises a photomultiplier tube, a direct-connection light guide convex seat and a direct-connection light guide concave seat; the photomultiplier is arranged on a direct-connection light guide convex seat, and the direct-connection light guide convex seat is arranged on a direct-connection light guide concave seat; the direct-connection light guide convex seat is connected with a synchronous belt in the reading Y-axis synchronous wheel component, and the direct-connection light guide concave seat is connected with a sliding block in the reading Y-axis guide rail sliding block component; the bar code device is fixed on the reading Y-axis fixing plate through a corresponding fixing piece.
The full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer provided by the invention adopts reasonable layout of the inner chamber and the outer chamber, combines with compact and scientific design of related functional modules, has simpler and more compact structure, lower cost and high automation degree, is particularly suitable for using a ready-to-use reagent, can detect one or more samples at the same time, has less sample consumption, is simple, convenient and quick to operate, can obtain a result within 30 minutes, greatly reduces the waiting time of a patient, and has more stable detection result; is very suitable for use, popularization and popularization of domestic medical units in China.
Drawings
FIG. 1 is a perspective view of an embodiment of the fully automatic magnetic bead time-resolved fluoroimmunoassay analyzer of the present invention (with the dark chamber housing removed);
FIG. 2 is a perspective view of the pallet module of FIG. 1 of the present invention;
FIG. 3 is an enlarged perspective view of the pallet module body of FIG. 2 of the present invention (cut away from slot 1);
FIG. 4 is an enlarged perspective view of the magnet assembly and magnet motor assembly of FIG. 3 in accordance with the present invention;
FIG. 5 is an enlarged perspective view of the test strip used in FIG. 2 according to the present invention;
FIG. 6 is an enlarged perspective view of the piercing pipetting module of FIG. 1 according to the invention;
FIG. 7 is an enlarged perspective view of the pipette tip assembly of FIG. 6 in accordance with the present invention;
FIG. 8 is an enlarged perspective view of the needle retraction selection assembly of FIG. 6 in accordance with the present invention;
FIG. 9 is an enlarged perspective view of the dark room door assembly of FIG. 1 of the present invention;
FIG. 10 is an enlarged perspective view of the code-scanning reading module of FIG. 1 according to the present invention.
Detailed Description
The embodiments and examples of the present invention will be described in detail below with reference to the accompanying drawings, and the described embodiments are only for the purpose of illustrating the present invention and are not intended to limit the embodiments of the present invention.
FIG. 1 is a perspective view of an embodiment of the fully automatic magnetic bead time-resolved fluoroimmunoassay analyzer (with the darkroom housing removed) of the present invention, as shown in FIG. 1, which comprises a rack 1, a tray module 2, a piercing pipetting module 4, a darkroom housing (not shown), a darkroom door 5 and a code-scanning reading module 6; wherein:
the rack 1 is formed by connecting a horizontal bottom plate 11 and a vertical support plate assembly 12 into an inverted T shape, and the whole instrument is divided into an inner chamber and an outer chamber; the support plate assembly 12 is of a frame structure consisting of a left side plate, a right side plate, a vertical plate and an upper plate of the vertical plate; the bottom of left side board and right side board is fixed respectively in the both sides of bottom plate 11, the riser setting is between left side board and right side board, and the below of riser leaves the breach of dodging of being convenient for tray module 2 to go into outer indoor lower floor space, the riser top piece is located the top of riser to be connected with the top of left side board and right side board.
An electronic component box 7 is fixed on the support plate component 12 and used for mounting related electronic components such as a power supply, a circuit board, a cooling fan, a switch button and the like, and the electronic component box 7 is positioned on the upper layer of the inner chamber and belongs to the inactive area of an instrument; this electronic components box 7 comprises power folded plate, fan folded plate and jam-proof board, the both sides of power folded plate are triangle-shaped, and its front end sets up on the left side board and the right side board of mounting panel subassembly 12, the fan folded plate is located the rear end of power folded plate, the jam-proof board is located the top of power folded plate.
The tray module 2 is positioned in the lower layer area of the outer chamber and used for placing and fixing a plurality of reagent strips 3 to be detected and feeding the reagent strips 3 into the lower layer area of the inner chamber or withdrawing the reagent strips 3 to the lower layer area of the outer chamber;
the puncture pipetting module 4 is positioned in the upper layer area of the outer chamber, can realize the whole automatic lifting, automatic loading or separation of a pipette tip through program control, and is used for automatically puncturing a thin film covering the top surface of a reagent tube on the reagent strip 3 and automatically sucking or discharging liquid in the reagent tube;
the darkroom shell is positioned in the lower layer area of the inner chamber and is used for forming a darkroom space for shielding or blocking external light, and the code scanning reading module 6 has strict requirements on the shielding of the light; to facilitate the display of the internal structure of the lower region of the instrument chamber, fig. 1 shows the dark chamber housing parts removed;
the darkroom door device 5 is arranged on a vertical plate of the bracket plate component 12 and is used for forming a darkroom space with one openable side by matching with a darkroom shell;
the code scanning reading module 6 is positioned in the lower layer area of the inner chamber and is positioned in the inner part of the darkroom shell, and can realize automatic movement of an X axis and a Y axis in the horizontal direction through program control, so as to excite and read the fluorescence information of the reagent strip 3 after incubation.
The full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer adopts reasonable layout of the inner chamber and the outer chamber, combines with compact and scientific design of related functional modules, has simpler and more compact structure and lower cost, improves the integral automation degree of the analyzer, is particularly suitable for using a ready-to-use reagent, can detect one or more samples simultaneously, and is very suitable for being used, popularized and popularized by medical units in China.
In a preferred embodiment of the full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer of the present invention, as shown in fig. 2, fig. 2 is a perspective view of the tray module of fig. 1 of the present invention, specifically, the tray module 2 is composed of a tray module power mechanism 21 and a tray module body 22, the tray module body 22 is installed on the tray module power mechanism 21, and the tray module power mechanism 21 is configured to feed the tray module body 22, which is installed with a plurality of reagent strips 3 side by side, into a lower layer region of the inner chamber or to exit into a lower layer region of the outer chamber.
Specifically, the tray module power mechanism 21 includes a tray stepping motor 211, two tray slide rails 212, an encoder 214, and a tray synchronous belt assembly 215; the tray synchronous belt assembly 215 is positioned above one of the tray slide rails 212, the tray module body 22 is erected above the two tray slide rails 212, and one side of the tray module body 22 is connected with the synchronous belt in the tray synchronous belt assembly 215 through a corresponding connecting piece, and is used for moving back and forth along the length direction of the tray slide rails 212 under the driving of the tray synchronous belt assembly 215; the tray stepping motor 211 and the encoder 214 are respectively located at two ends of the tray synchronous belt assembly 215, are fixed at two ends of the tray sliding rail 212 below the tray synchronous belt assembly 215 through respective fixing pieces, and are in transmission connection with the synchronous belt in the tray synchronous belt assembly 215 through a synchronous belt pulley and a corresponding bearing in the tray synchronous belt assembly 215; the encoder 214 is used for cooperating with the tray stepping motor 211 to stop the tray module body 22 at a position which needs to be reached, when the encoder 214 detects that the tray stepping motor 211 runs to the position which needs to be reached by the tray module body 22, a feedback signal is sent to the circuit board in the electronic component box 7 to stop sending a pulse signal to the tray stepping motor 211, so that when the darkroom door device 5 is opened, the tray module body 22 with the reagent strips 3 passes through an avoiding notch at the bottom of a vertical plate in the support plate assembly 12 and is sent to an inner chamber lower layer area or exits to an accurate position of an outer chamber lower layer area.
Referring to fig. 3 and 4, fig. 3 is an enlarged perspective view of the tray module body of fig. 2 (cut away from the 1 st slot) according to the present invention, and fig. 4 is an enlarged perspective view of the magnet assembly and the magnet power mechanism of fig. 3 according to the present invention; specifically, the tray module body 22 includes a tray bottom plate 221, a needle tube holder 222, a reagent strip tray 223, a reagent strip pressing plate 224, a reagent strip clip 228, a heating plate 225, a heating plate fixing plate 2251, a magnet assembly 226, a magnet assembly power mechanism 227, and a magnet up-down optical coupler assembly 229; specifically, the method comprises the following steps:
the tray bottom plate 221 is erected on two tray slide rails 212 of the tray module power mechanism 21 shown in fig. 2, and a connecting member (shown in fig. 2) for connecting with a synchronous belt in the tray synchronous belt assembly 215 is fixed on one side of the tray bottom plate 221;
the needle tube support block 222 and the reagent strip tray 223 are fixed above the tray bottom plate 221 through corresponding left and right support pieces in FIG. 2; a plurality of rows of needle holes 2221 for placing three needles 2222 are correspondingly arranged on the needle tube holder 222, each row of needle holes 2221 is located on an extension line of the rear end of the opening slot 2231 corresponding to the needle tube holder, a plurality of sample holes 2223 for placing test tubes (not shown) containing samples to be detected (nucleic acid) are also arranged on the needle tube holder 222, each sample hole 2223 is also located on an extension line of the rear end of the opening slot 2231 corresponding to the sample hole 2223, and the needle holes 2221 arranged in rows are located between the opening slot 2231 corresponding to the sample hole 2223;
a plurality of open grooves 2231 adapted to be placed in the reagent strips 3 shown in fig. 2 are arranged in parallel on the reagent strip tray 223 to limit the movement of the reagent strips 3 in the left-right direction; the reagent strip pressing pieces 224 are distributed on the top surfaces of the reagent strip trays 223 at both sides of the open slot 2231, and are used for pressing the reagent strips 3 pushed into the open slot 2231 to limit the movement of the reagent strips 3 in the up-and-down direction; the reagent strip clip 228 is located on the bottom surface of the reagent strip tray 223 at the front end of the open slot 2231, and is used for clamping the outer wall of the test tube at the foremost end of the reagent strip 3 to limit the movement of the reagent strip 3 in the front-back direction.
The heating plate 225 is attached to the bottom surface of the heating plate fixing plate 2251, the heating plate fixing plate 2251 is located at the rear of the opening slot 2231 and is fixed below the reagent strip tray 223, a plurality of heating protrusions 2252 are spaced upward from the top surface of the heating plate fixing plate 2251, and heat generated by the heating plate 225 is conducted to the heating protrusions 2252 through the heating plate fixing plate 2251 to heat and keep the temperature of the reagent tubes located between the two heating protrusions 2252 on the reagent strip 3 constant; the heater chip 225 is powered on to heat when the device is powered on, and the plurality of heating protrusions 2252 on the heater chip fixing plate 2251 are maintained at 37 ℃ until the device is powered off.
The magnet assembly 226, the magnet assembly power mechanism 227 and the magnet up-down optical coupler assembly 229 are all positioned between the tray bottom plate 221 and the reagent strip tray 223, and the magnet assembly 226 is positioned below the heating plate fixing plate 2251 and the heating plate 225; the magnet assembly power mechanism 227 is used for raising or lowering the magnet assembly 226 to complete the magnetic bead separation step of the reagent strip 3; the magnet up-down optical coupler 229 is installed between the magnet assembly 226 and the magnet assembly power mechanism 227, and is used for detecting whether the position of the magnet assembly 226 in the tray module body 22 is in the up position or the down position.
Specifically, the magnet assembly 226 includes a magnet holder 2261, a plurality of large magnets 2262, two guide posts 2263 and two guide post springs 2264; the bottoms of the two guide posts 2263 are fixed on the tray bottom plate 221, the magnet fixing seat 2261 is sleeved on the two guide posts 2263 through corresponding linear bearings and can move up and down, and the two guide post springs 2264 are respectively sleeved on the two guide posts 2263 and abut against between the magnet fixing seat 2261 and the heating plate fixing plate 2251 to assist the magnet fixing seat 2261 to move down; the large magnets 2262 are cuboid and magnetic, all the large magnets 2262 are vertically arranged on the magnet fixing seat 2261 at intervals and are positioned on an extension line at the rear end of the opening groove 2231 corresponding to the large magnets, and the top of each large magnet 2262 is intersected by two inclined top surfaces along the length direction of the opening groove 2231 to form a conical surface; correspondingly, a magnet through hole 2253 is formed on the heating plate fixing plate 2251 between the two heating protrusions 2252 and is adapted to the corresponding large magnet 2262 to pass through, so that the magnet fixing seat 2261 drives the large magnet 2262 to ascend or descend.
Specifically, the magnet assembly power mechanism 227 comprises a motor mounting plate 2271, a dc brushless motor 2272, an eccentric disc 2273, a bearing and a connecting shaft 2274 thereof; the motor mounting plate 2271 is vertically mounted on the tray bottom plate 221, and the large surface of the motor mounting plate 2271 faces the magnet fixing seat 2261; the dc brushless motor 2272 is transversely fixed on the motor mounting plate 2271, and the output shaft of the dc brushless motor 2272 passes through the motor mounting plate 2271 and faces the magnet fixing seat 2261; the eccentric disc 2273 is connected to the output shaft of the dc brushless motor 2272, the bearing and the connecting shaft 2274 thereof are fixed on the disc surface of the eccentric disc 2273, and the fixed position deviates from the output shaft of the dc brushless motor 2272; one side of the magnet fixing seat 2261, which faces the magnet assembly power mechanism 227, is transversely provided with an adaptive bearing and a long round hole 2275 in which a connecting shaft 2274 of the adaptive bearing rolls, so that the magnet fixing seat 2261 is stirred to drive the large magnet 2262 to ascend or descend under the driving of the direct-current brushless motor 2272; in order to overcome the defect that the eccentric wheel transmission mechanism has a dead lock point, the two guide post springs 2264 can also play a role in preventing the eccentric disc 2273 from being clamped in the rotating process of the direct current brushless motor 2272.
Referring to fig. 5, fig. 5 is an enlarged perspective view of the reagent strip used in fig. 2 of the present invention, and specifically, the reagent strip 3 is formed by sequentially arranging, from left to right, a first inclined reagent tube 311, a second inclined reagent tube 312, a first cylindrical detachable reagent tube sleeve 321, a second cylindrical detachable reagent tube sleeve 322, a first cylindrical reagent tube 331, a first elliptical reagent tube 341, a second elliptical reagent tube 342, a second cylindrical reagent tube 332, a third cylindrical reagent tube 333, a fourth cylindrical reagent tube 334, a fifth cylindrical reagent tube 335, a sixth cylindrical reagent tube 336, and a seventh cylindrical reagent tube 337; wherein, the first bevel reagent tube 311 and the second bevel reagent tube 312 are located at the tail of the reagent strip 3 for magnetic bead separation, and the first cylindrical detachable reagent sleeve 321 and the second cylindrical detachable reagent sleeve 322 are respectively used for loading the first cylindrical detachable reagent tube and the second cylindrical detachable reagent tube (both not shown in fig. 5); when the reagent strip 3 is pushed in, the reagent strip clip 228 of the tray module body 22 can clip the outer wall of the seventh cylindrical reagent tube 337 at the head of the reagent strip 3.
Referring to fig. 6, fig. 6 is an enlarged perspective view of the piercing pipetting module of fig. 1, specifically, the piercing pipetting module 4 includes a piercing pipetting stepping motor 41, a piercing pipetting synchronizing belt assembly 42, a piercing pipetting screw transmission assembly 43, a pipetting withdrawing assembly 44, a withdrawing needle head selection assembly 45, a piercing pipetting guide rail slide assembly (not shown) and a large slide plate 46; specifically, the method comprises the following steps:
the pipetting and withdrawing assembly 44 and the needle withdrawing head selection assembly 45 are both arranged at the middle lower part of the front surface of the large sliding plate 46; the back of the large sliding plate 46 is connected with a sliding block in the puncturing pipetting guide rail sliding block assembly through screws, and two guide rails in the puncturing pipetting guide rail sliding block assembly are vertically fixed on the front of a vertical plate in the support plate assembly 12 in fig. 1 at intervals through screws; the puncture pipetting stepping motor 41 is vertically installed on the back of the vertical plate in the bracket plate assembly 12 in fig. 1 through a corresponding fixing part, and the output shaft of the puncture pipetting stepping motor 41 is arranged upwards and is connected with one synchronous pulley in the puncture pipetting synchronous belt assembly 42 through a corresponding bearing;
the screw in the piercing pipetting screw driving assembly 43 is also vertically mounted on the back of the vertical plate in the rack plate assembly 12 in fig. 1 through a corresponding bearing and a fixing piece, and the upper end of the screw is connected with another synchronous pulley in the piercing pipetting synchronous belt assembly 42 through a corresponding bearing; the nut in the piercing pipetting screw driving assembly 43 is mounted on the back of the large sliding plate 46 via a corresponding fixing member, and the nut is located on the longitudinal central axis of the large sliding plate 46; the synchronous belt in the puncture pipetting synchronous belt assembly 42 is sleeved on the two synchronous belt wheels and is used for driving the screw rod to rotate under the driving of the puncture pipetting stepping motor 41, and the large sliding plate 46 and the sliding block on the back side thereof are driven to slide up and down along the guide rail through the nut matched with the screw rod, so that the pipetting and withdrawing assembly 44 and the needle withdrawing head selection assembly 45 which are arranged on the front side of the large sliding plate 46 are integrally moved up and down, and the actions of puncture, pipetting and/or needle withdrawing head selection and the like are finished according to the detection requirement.
Referring to fig. 7 and 8, fig. 7 is an enlarged perspective view of the pipette tip ejection assembly of fig. 6 of the present invention, and fig. 8 is an enlarged perspective view of the needle tip ejection selection assembly of fig. 6 of the present invention; specifically, the pipette and pipette out assembly 44 comprises a pipette and pipette out motor fixing plate 442, a pipette and pipette out screw motor 441, a pipette and pipette out guide rail slide block assembly 444, a small slide plate 451, an injector push plate 452, a pipette and pipette out motor nut 458, a plurality of puncture needles 456, an injector fixing plate 443, a plurality of pipette injectors 445 and an optical coupling sensor 457; specifically, the method comprises the following steps:
the pipette and withdrawal motor fixing plate 442 is transversely and vertically fixed on the front surface of the large sliding plate 46, the pipette and withdrawal screw motor 441 is mounted on the pipette and withdrawal motor fixing plate 442, and the screw of the pipette and withdrawal screw motor 44 penetrates through the pipette and withdrawal motor fixing plate 442 and is vertically and downwardly arranged; two guide rails in the pipette-withdrawal guide rail sliding block assembly 444 are vertically fixed on the front surface of the large sliding plate 46 below the pipette-withdrawal motor fixing plate 442 at intervals through screws, the back surface of the small sliding plate 451 is connected with the sliding blocks in the pipette-withdrawal guide rail sliding block assembly 444, the injector push plate 452 is fixed on the top of the small sliding plate 451, and the pipette-withdrawal motor nut 458 is mounted on the injector push plate 452 and is matched with the screw of the pipette-withdrawal screw motor 441 for driving the injector push plate 452, the small sliding plate 451 and the sliding blocks on the back surface thereof to slide up and down along the guide rails through the pipette-withdrawal motor nut 458 matched with the screw under the driving of the pipette-withdrawal screw motor 44; a plurality of puncture needles 456 are vertically fixed on the bottom surface of the small sliding plate 451 at intervals for completing the puncture action according to the detection requirement;
the syringe fixing plate 443 is positioned below the pipette-withdrawing motor fixing plate 442 and is connected with the pipette-withdrawing motor fixing plate 44 through two upright posts; a plurality of pipetting syringes 445 are vertically arranged on the top surface of the syringe fixing plate 443 at intervals, and a plurality of clamping grooves which are adaptive to be clamped at the tail parts of the pipetting syringes 445 are arranged on the front edge of the syringe push plate 452 and are used for completing pipetting according to detection requirements;
the opto-coupler sensor 457 is connected to the front surface of the large slide plate 46 below the pipette-retracting motor fixing plate 442 via corresponding fixing members, and is used for sensing whether the syringe push plate 452 is located at the upper position or the lower position.
Specifically, the needle withdrawing head assembly 45 comprises a plurality of needle withdrawing columns 446, a plurality of needle withdrawing plates 447, a needle withdrawing stepping motor 453, a needle withdrawing synchronization belt assembly 454 and a needle withdrawing guide rail sliding block assembly 455; two needle withdrawing columns 446 are respectively arranged on the syringe fixing plate 443 behind each pipetting syringe 445 through corresponding linear bearings, a spring is sleeved on the upper half part of each needle withdrawing column 446 for enabling the needle withdrawing column 446 inside the spring to restore to an initial position under the condition of no external force, the lower ends of the two needle withdrawing columns 446 are respectively connected with a needle withdrawing plate 447, and the front end of the needle withdrawing plate 447 is provided with a notch which is matched with the needle withdrawing of the pipetting syringe 445;
the needle withdrawing step motor 453 is connected to one side of the small sliding plate 451 laterally inward via a corresponding fixing member, and an output shaft of the needle withdrawing step motor 453 is connected to one timing pulley of the needle withdrawing synchronization belt assembly 454 via a corresponding bearing, and the other timing pulley of the needle withdrawing synchronization belt assembly 454 is connected to the other side of the small sliding plate 451 via a corresponding bearing and a connecting shaft; the guide rail of the needle withdrawing guide rail sliding block assembly 455 is transversely fixed at the lower end of the front surface of the small sliding plate 451 through a screw, the sliding block of the needle withdrawing guide rail sliding block assembly 455 is connected with a synchronous belt in the needle withdrawing synchronous belt assembly 454 through a corresponding connecting piece, the synchronous belt is sleeved on two synchronous pulleys in the needle withdrawing synchronous belt assembly 454, and the synchronous belt is used for driving the sliding block to move along the guide rail to the positions above two needle withdrawing columns 446 behind the pipetting injector 445 requiring needle withdrawing through the synchronous belt under the driving of the needle withdrawing stepping motor 453, and the small sliding plate 451 and the sliding block are driven to move downwards through a nut under the driving of the pipetting screw motor 441 so as to press the corresponding two needle withdrawing columns 446 and overcome the elastic forces of two springs to link the downward movement of the needle withdrawing columns 446 at the lower ends of the two needle withdrawing columns 446, so as to finish the action of withdrawing the needle head of the pipetting injector, and when the small sliding block 451 and the sliding block move upwards, the elastic forces of the two springs sleeved on the two needle withdrawing columns 446 make the two needle withdrawing columns 446 move upwards and the withdrawing columns return the needle withdrawing plates 447 connecting the lower ends of the small sliding block.
Referring to fig. 9, fig. 9 is an enlarged perspective view of the darkroom door apparatus of fig. 1 of the present invention, in particular, the darkroom door apparatus 5 comprises a darkroom door 51, a two-darkroom door guide rail slider assembly (not shown) and a darkroom door power mechanism 52; the back of the said darkroom door 51 is equipped with the recess 511 and slide block attachment hole 512 which are fit to embed into the slide block assembly of the two darkroom door guiderails respectively, the darkroom door 51 is connected to the slide block in the sliding block assembly of the darkroom door guiderail by passing the screw through these slide block attachment holes 512, the guiderail in the sliding block assembly of the darkroom door guiderail is fixed on the front of the vertical plate of the bracket plate assembly 12 of fig. 1 vertically by the screw; one side of the darkroom door 51 is connected with the darkroom door power mechanism 52 through the corresponding darkroom door connector 522, and is used for being driven by the darkroom door power mechanism 52 to ascend or descend along the guide rail through the slider so as to be matched with the darkroom shell to open or close the space of the lower layer area of the inner chamber of the instrument, and comprises an avoidance notch which is used for opening or shielding the lower part of the vertical plate in the support plate assembly 12 to avoid the tray module 2 from entering or exiting the lower layer space of the inner chamber and the outer chamber, so that the darkroom space can be opened or closed; the closed state can play a role in sealing a darkroom space, and the open state can meet the requirement that the tray module body 22 provided with the reagent strips 3 can enter and exit between the lower layer area of the inner chamber and the lower layer area of the outer chamber of the instrument.
Specifically, the darkroom door power mechanism 52 is composed of a darkroom door stepping motor 521, the aforementioned darkroom door connector 522, a darkroom door synchronous belt assembly 523 and a darkroom door power mechanism support assembly 524; the timing belt in the chamber door timing belt assembly 523 is vertically positioned at one side of the chamber door 51, and the chamber door connecting piece 522 is connected between the timing belt and the chamber door 51 through a screw; at the upper end of the timing belt, the darkroom door stepping motor 521 is fixed on the front face of the vertical plate of the bracket plate assembly 12 in fig. 1 through a darkroom door stepping motor support in a darkroom door power mechanism support assembly 524, and a timing pulley in the darkroom door timing belt assembly 523 is connected to the output shaft of the darkroom door stepping motor 521 through a corresponding bearing; at the lower end of the synchronous belt, a synchronous belt support in the darkroom door power mechanism support assembly 524 is fixed on the front side of the vertical plate of the bracket plate assembly 12 in fig. 1, and another synchronous pulley in the darkroom door synchronous belt assembly 523 is connected to the synchronous belt support through a corresponding bearing and a connecting shaft; the synchronous belt is sleeved on the two synchronous belt wheels and used for driving the darkroom door 51 to move up and down through the darkroom door connecting piece 522 under the driving of the darkroom door stepping motor 521.
Referring to fig. 10, fig. 10 is an enlarged perspective view of the code scanning value reading module of fig. 1, in particular, the value reading module 6 is composed of a value reading X-axis power mechanism 61, a value reading Y-axis power mechanism 62, a lens assembly 63 and a bar code device 64; the read value X-axis power mechanism 61 and the read value Y-axis power mechanism 62 are respectively used for moving the lens assembly 63 in the X-axis direction and the Y-axis direction in the horizontal plane, and the read value X-axis power mechanism 61 is also used for moving the barcode reader 64 while moving the lens assembly 63 in the X-axis direction in the horizontal plane; the lens assembly 63 can achieve effective light-emitting data collection of reagent tubes needing to be read on the plurality of reagent strips 3 placed in the tray module 2 in fig. 1 through program control, and the barcode reader 64 is used for reading barcode information on the plurality of reagent strips 3.
Specifically, the value reading X-axis power mechanism 61 includes a value reading X-axis stepping motor 611, a value reading X-axis synchronizing wheel assembly 612, a value reading X-axis guide rail slider assembly 613 and a value reading X-axis fixing plate 614; wherein, the reading X-axis fixing plate 614 is erected on the bottom plate 11 of the frame 1 in FIG. 1 through two upright posts; the X-axis stepping motor 611 is installed on the front surface of the vertical plate in the supporting plate assembly 12 in fig. 1 along the Y-axis direction through a corresponding fixing member, an output shaft of the X-axis stepping motor 611 passes through the vertical plate and is connected with one synchronous pulley in the value reading X-axis synchronous wheel assembly 612 through a corresponding bearing, and another synchronous pulley in the value reading X-axis synchronous wheel assembly 612 is connected on the back surface of the vertical plate in the supporting plate assembly 12 in fig. 1 through a corresponding bearing, a connecting shaft and a fixing member; the guide rail in the reading X-axis guide rail slider assembly 613 is fixed on the reading X-axis fixing plate 614 between the two synchronous pulleys by screws along the X-axis direction, the bottom surface of one end of the reading Y-axis fixing plate 624 in the reading Y-axis power mechanism 62 is connected with the slider in the reading X-axis guide rail slider assembly 613, and the top surface of one end of the reading Y-axis fixing plate 624 is connected with the synchronous belt in the reading X-axis synchronous pulley assembly 612 by corresponding connecting pieces, so that the reading Y-axis power mechanism 62, the lens assembly 63 thereon and the bar code device 64 are driven by the X-axis stepping motor 611 to move back and forth along the X-axis direction by the synchronous belt of the reading X-axis synchronous pulley assembly 612.
Specifically, the reading Y-axis power mechanism 62 includes a reading Y-axis stepping motor 621, a reading Y-axis synchronizing wheel assembly 622, a reading Y-axis guide rail slider assembly 623, the reading Y-axis fixing plate 624, a reading Y-axis roller 625, and a reading Y-axis supporting plate 615; the reading Y-axis support plate 615 is also erected on the bottom plate 11 of the rack 1 in fig. 1 via two vertical columns, and the reading Y-axis roller 625 is mounted at the other end of the reading Y-axis fixing plate 624 and used for rolling back and forth on the reading Y-axis support plate 615; the Y-axis stepping motor 621 is mounted at the roller end of the value-reading Y-axis fixing plate 624 along the X-axis direction via a corresponding fixing member, and the output shaft of the Y-axis stepping motor 621 is connected with one synchronous pulley in the value-reading Y-axis synchronous pulley assembly 622 via a corresponding bearing, and the other synchronous pulley in the value-reading Y-axis synchronous pulley assembly 622 is fixed at the other end of the value-reading Y-axis fixing plate 624 via a corresponding bearing, a connecting shaft and a fixing member; the guide rail in the value reading Y-axis guide rail sliding block component 623 is fixed on the value reading Y-axis fixing plate 624 between the two synchronous belt wheels along the Y-axis direction through a screw, the lens component 63 is connected with the sliding block in the value reading Y-axis guide rail sliding block component 623, and the lens component 63 is connected with the synchronous belt in the value reading Y-axis synchronous wheel component 622, and is used for driving the lens component 63 to move back and forth along the Y-axis direction through the synchronous belt of the value reading Y-axis synchronous wheel component 622 under the driving of the value reading Y-axis stepping motor 621.
Specifically, the lens assembly 63 includes a photomultiplier tube (PMT) 631, a directly coupled light guide boss, and a directly coupled light guide recess 632; the photomultiplier tube (PMT) 631 is mounted on a directly connected light guide boss mounted on a directly connected light guide dimple 632; the direct-connection light guide convex seat is connected with the synchronous belt in the reading Y-axis synchronous wheel component 622, and the direct-connection light guide concave seat 632 is connected with the slide block in the reading Y-axis guide rail slide block component 623.
The barcode reader 64 is fixed to a reading Y-axis fixing plate 624 of the reading Y-axis power mechanism 62 via a corresponding fixing member.
In the following, taking the conventional blood nucleic acid sample detection as an example, the working principle and the detection process of the embodiment of the full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer of the present invention are as follows:
step S800, respectively putting 1 to 5 test tubes filled with blood (nucleic acid) samples to be detected into a plurality of sample holes 2223 on the needle tube support block 222 in the figure 3, respectively putting 1 to 5 reagent strips 3 into corresponding open slots 2231 and pushing the reagent strips forward tightly, so that the outer walls of the test tubes (namely, seventh cylindrical reagent tubes 337 in the figure 5) at the foremost ends of the reagent strips 3 are respectively clamped by 1 to 5 reagent strip clips 228.
Step S810, starting a corresponding detection switch on the instrument, and driving the darkroom door 51 to move upwards by the darkroom door stepping motor 521 in fig. 9; the tray stepping motor 211 in fig. 2 drives the tray module body 22 to move the reagent strips 3 to the lower space of the inner chamber of the instrument along the tray slide rail 212, the barcode reader 64 is driven to move along the X-axis direction by the X-axis stepping motor 611 in fig. 10, and barcodes on 1 to 5 reagent strips 3 are sequentially read for confirmation; then, the tray stepping motor 211 shown in fig. 2 drives the tray module body 22 to move the reagent strip 3 to the lower part of the piercing and pipetting module 4, and the piercing and pipetting stepping motor 41 shown in fig. 6 drives the large sliding plate 46 to move downwards, so that 1 to 5 piercing needles 456 at the lower part of the needle withdrawing selection assembly 45 sequentially pierce the films sealed on the top surfaces of all the reagent tubes on the reagent strip 3.
Step S820, the instrument loads the blood (nucleic acid) samples in the sample wells 2223 corresponding to the sample wells into the first inclined reagent tube 311 and the second inclined reagent tube 312 on the reagent strip 3 in the open slot 2231 corresponding to the reagent strip tray 223 of fig. 3 respectively through 1 to 5 pipette syringes 445 installed on the top surface of the syringe fixing plate 443 of fig. 7, and mixes (or stirs) the blood (nucleic acid) samples with the magnetic beads in the first inclined reagent tube 311 and the second inclined reagent tube 312; specifically, the pipette and withdrawal pipe screw motor 441 in fig. 7 drives the injector push plate 452 in fig. 8 to move up and down, and drives 1 to 5 pipette injectors 445 to complete suction and discharge actions, so as to realize pipetting and mixing (or stirring), wherein the mixing (or stirring) is realized by multiple suction and discharge of the pipette injectors 445.
It should be noted that, since only three needles 2222 are available in the needle holes 2221 in the same row in fig. 3, for this reason, the same needle 2222 is used in connection with the first inclined reagent tube 311 of each reagent strip 3, and the same needle 2222 is used in connection with the pipetting and aspirating operations of the first inclined reagent tube 311; similarly, another needle 2222 is used in association with each reagent strip 3 second angled reagent tube 312; the remaining third needle 2222 is dedicated to enhanced fluid pipetting and aspirating.
In step S820, each pipetting syringe 445 is used to load the blood (nucleic acid) sample to be detected into the first inclined reagent tube 311 and the second inclined reagent tube 312 of the corresponding reagent strip 3 by replacing the respective needle 2222, and mix the sample with the magnetic beads therein by multiple pipetting.
When the needle 2222 is installed, the tray module body 22 is driven by the tray stepping motor 211 shown in fig. 2 to move to the position below the puncture pipetting module 4, the needle 2222 shown in fig. 3 is positioned right below the pipetting syringe 445 shown in fig. 7, the puncture pipetting stepping motor 41 shown in fig. 6 drives the large sliding plate 46 to move downwards, the heads of 1 to 5 pipetting syringes 445 move downwards, and then the corresponding needles 2222 are installed.
When withdrawing the needle 2222, the needle withdrawing motor 453 of fig. 8 drives the slide block in the needle withdrawing guide slide block assembly 455 to move along its guide rail, and as shown in fig. 7, the slide block moves over two needle withdrawing columns 446 at the rear of the corresponding pipetting syringe 445, and the pipetting needle withdrawing screw motor 441 drives the needle withdrawing guide slide block assembly 455 to move downward relative to the pipetting needle withdrawing assembly 44, so that the slide block in the needle withdrawing guide slide block assembly 455 presses the two needle withdrawing columns 446 at the lower part thereof, thereby driving the needle withdrawing plate 447 to move downward, thereby withdrawing the needle 2222 mounted at the head of the corresponding pipetting syringe 445, and the withdrawn needle 2222 is still placed in the original needle hole 2221 to mount another needle 2222, thereby achieving the purpose of replacing the needle 2222.
In step S830, each pipetting syringe 445 is replaced by its own needle 2222, and the neutralizers in the fourth cylindrical reagent tube 334 and the fifth cylindrical reagent tube 335 of the corresponding reagent strip 3 are loaded into the first bevel reagent tube 311 and the second bevel reagent tube 312, respectively, and are mixed by multiple pipetting and placing.
Step S840, after a specified period of incubation time, the dc brushless motor 2272 shown in fig. 4 drives the magnet fixing base 2261 to move upward, and the 1 to 5 large magnets 2262 rise to a position between the first inclined reagent tube 311 and the second inclined reagent tube 312 of the corresponding reagent strip 3, so as to magnetically attract the magnetic beads in the first inclined reagent tube 311 and the second inclined reagent tube 312 and move to the inclined surfaces thereof.
Step S850, each pipetting syringe 445 sucks the reaction liquid in the first bevel reagent tube 311 and the second bevel reagent tube 312 of the corresponding reagent strip 3 respectively by replacing the respective needle 2222, and discharges the reaction liquid onto the magnetic beads on the respective bevels; this step is repeated several times.
Step S860, each pipetting syringe 445 sucks the cleaning solutions in the first elliptic reagent tube 341 and the second elliptic reagent tube 342 of the corresponding reagent strip 3 respectively by replacing the respective needle 2222, and cleans the magnetic beads on the respective slopes; the step also needs to be repeated for a plurality of times, and the washing is repeatedly carried out; and during the last cleaning, the direct current brushless motor 2272 shown in fig. 4 drives the magnet fixing base 2261 to move downwards, the large magnets 2262 with 1 to 5 blocks descend, and the liquid-transferring syringe 445 washes the magnetic beads on the inclined surfaces of the large magnets with cleaning liquid to the bottom of the reagent tube and sucks away the cleaned waste liquid.
It should be noted that the waste liquid after washing can be placed in a useless reagent tube on the corresponding reagent strip, such as the useless seventh cylindrical reagent tube 337, the spare second cylindrical reagent tube 332 and third cylindrical reagent tube 333, and the used fourth cylindrical reagent tube 334 and fifth cylindrical reagent tube 335 for containing the neutralizing agent.
Step S870, each pipetting injector 445 adds the marker diluent in the first cylindrical reagent tube 331 of the corresponding reagent strip 3 to the markers in the first cylindrical detachable reagent tube 321 and the second cylindrical detachable reagent tube 322 of the corresponding reagent strip 3 by replacing the respective needle 2222, and mixes the marker diluent by sucking and releasing for a plurality of times; wherein the needle 2222 associated with the first inclined reagent tube 311 is used for sucking and discharging the diluted marker in the first cylindrical detachable reagent tube 321, and the needle 2222 associated with the second inclined reagent tube 312 is used for sucking and discharging the diluted marker in the second cylindrical detachable reagent tube 322.
In step S880, each pipette syringe 445 sucks the diluted markers in the first cylindrical detachable reagent tube 321 and the second cylindrical detachable reagent tube 322 to add to the corresponding first bevel reagent tube 311 and the second bevel reagent tube 312 by replacing the respective needle 2222, and mixes the diluted markers by multiple sucking and discharging.
Step S890, the dc brushless motor 2272 shown in fig. 4 drives the magnet fixing base 2261 to move upward, and 1 to 5 large magnets 2262 are lifted to a position between the first bevel reagent tube 311 and the second bevel reagent tube 312 of the corresponding reagent strip 3, so as to magnetically attract the magnetic beads in the first bevel reagent tube 311 and the second bevel reagent tube 312 and move to the bevel thereof. (same as step S840)
Step S900, each pipetting syringe 445 sucks the reaction liquid in the first bevel reagent tube 311 and the second bevel reagent tube 312 of the corresponding reagent strip 3 respectively by replacing the respective needle 2222 and discharges the reaction liquid onto the magnetic beads on the respective bevels; this step is repeated several times. (same as step S850)
Step S910 is to wash the magnetic beads in the first and second sloped reagent tubes 311 and 312 again, and repeat step S860.
Step S920, each pipetting syringe 445 is replaced with a third needle 2222 dedicated for pipetting enhancement solutions, pipetting enhancement solutions in the sixth cylindrical reagent tube 336, loading the enhancement solutions into the first bevel reagent tube 311 and the second bevel reagent tube 312, and mixing the enhancement solutions by pipetting and pipetting for multiple times.
Step S930, the tray stepping motor 211 in fig. 2 drives the tray module body 22 to bring the incubated reagent strip 3 into the lower space of the inner chamber of the instrument along the tray slide rail 212; the dark room door 51 is driven by the dark room door stepping motor 521 of fig. 9 to move down, closing the space inside the dark room housing.
Step S940, the X-axis stepping motor 611 and the Y-axis stepping motor 621 read the value in fig. 10 drive the lens assembly 63 to stay above the reagent strip 3 to be read, and excite and read the fluorescence information of the first inclined-plane reagent tube 311 and the second inclined-plane reagent tube 312 of the reagent strip 3, so as to complete the collection of the luminescence data.
Step S940, the darkroom door 51 is driven by the darkroom door stepping motor 521 of fig. 9 to move up; the tray stepping motor 211 in fig. 2 drives the tray module body 22 to move the reagent strip 3 along the tray slide rail 212 to the lower space of the outer chamber of the apparatus; then the darkroom door 51 is driven by the darkroom door stepping motor 521 in fig. 9 to move downwards; the reagent strips 3 on the tray module body 22 are to be removed.
Therefore, when the full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer is used for detection, the whole analyzer can realize a series of automatic steps of puncturing, reagent adding, liquid sucking, stirring, cleaning, incubating, magnetic bead separation and darkroom luminescence detection of the reagent strip to be detected only by placing the reagent strip to be detected in the reagent strip tray and pushing the reagent strip to be detected into the reagent strip tray for clamping, so that the automation degree of the whole magnetic bead time-resolved fluorescence immunoassay analyzer is higher; one or more samples can be detected simultaneously, the sample dosage is less, the operation is simple, convenient and quick, the result can be obtained within 30 minutes, the waiting time of a patient is greatly reduced, and the detection result is more stable.
It should be understood that the above-mentioned embodiments are only preferred embodiments of the present invention, and are not intended to limit the technical solutions of the present invention, and those skilled in the art should understand that they can add, subtract, change or modify the above-mentioned descriptions within the spirit and principle of the present invention, and all the technical solutions of the add, subtract, change, modification or modify should belong to the protection scope of the appended claims.

Claims (9)

1. The utility model provides a full-automatic magnetic bead time-resolved fluorescence immunoassay appearance which characterized in that includes: the device comprises a rack, a tray module, a puncture liquid-transferring module, a darkroom shell, a darkroom door device and a code scanning and reading module; wherein: the frame divides the whole instrument into an inner chamber and an outer chamber; an electronic component box is fixed on the upper region of the inner chamber, a darkroom shell is positioned on the lower region of the inner chamber, and a darkroom door device is arranged on the rack and is used for forming a darkroom space with one side capable of being opened and closed together with the darkroom shell; the tray module is positioned in the lower layer area of the outer chamber and used for placing and fixing a plurality of reagent strips to be detected and feeding the reagent strips into the lower layer area of the inner chamber or withdrawing the reagent strips to the lower layer area of the outer chamber; the puncture liquid-moving module is positioned in the upper layer area of the outer chamber and is used for automatically puncturing a thin film on the reagent strip and covering the top surface of the reagent tube and automatically sucking or discharging liquid in the reagent strip; the code scanning reading module is positioned in the lower layer area of the inner chamber and is positioned in the dark room shell and used for exciting and reading the fluorescence information of the incubated reagent strip;
the puncture liquid-transfering module comprises a puncture liquid-transfering stepping motor, a puncture liquid-transfering synchronous belt assembly, a puncture liquid-transfering screw transmission assembly, a liquid-transfering tube-returning assembly, a needle-returning head selection assembly, a puncture liquid-transfering guide rail slide block assembly and a large sliding plate; the liquid transferring and withdrawing assembly and the needle withdrawing head selection assembly are both arranged at the middle lower part of the front surface of the large sliding plate; the back surface of the large sliding plate is connected with a sliding block in the puncture pipetting guide rail sliding block assembly through screws, and two guide rails in the puncture pipetting guide rail sliding block assembly are vertically fixed on the front surface of the rack at intervals through screws; the puncture pipetting stepping motor is vertically installed on the back of the rack through a corresponding fixing piece, and an output shaft of the puncture pipetting stepping motor is arranged upwards and is connected with one synchronous pulley in the puncture pipetting synchronous belt component through a corresponding bearing; the screw rod in the puncture pipetting screw rod transmission assembly is also vertically arranged on the back of the rack through a corresponding bearing and a fixing piece, and the upper end of the screw rod is connected with another synchronous belt wheel in the puncture pipetting synchronous belt assembly through a corresponding bearing; the nut in the puncture pipetting screw rod transmission assembly is arranged on the back surface of the large sliding plate through a corresponding fixing piece and is positioned on the longitudinal central axis of the large sliding plate; the synchronous belt in the puncture pipetting synchronous belt component is sleeved on the two synchronous belt wheels and is used for driving the screw rod to rotate under the driving of the puncture pipetting stepping motor and driving the large sliding plate and the sliding block on the back of the large sliding plate to slide up and down along the guide rail through the nut matched with the screw rod, so that the pipetting and withdrawing assembly and the withdrawing needle head selecting assembly which are arranged on the front surface of the large sliding plate integrally move up and down.
2. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 1, characterized in that: the tray module consists of a tray module power mechanism and a tray module body, the tray module body is arranged on the tray module power mechanism, and the tray module power mechanism is used for feeding the tray module body which is provided with a plurality of reagent strips side by side into the lower layer area of the inner chamber or withdrawing the tray module body to the lower layer area of the outer chamber; the tray module power mechanism comprises a tray stepping motor, two tray slide rails, an encoder and a tray synchronous belt assembly; the tray synchronous belt assembly is positioned above one of the tray slide rails, the tray module body is erected above the two tray slide rails, and one side of the tray module body is connected with a synchronous belt in the tray synchronous belt assembly through a corresponding connecting piece; the tray stepping motor and the encoder are respectively positioned at two ends of the tray synchronous belt assembly, are fixed at two ends of a tray sliding rail below the tray synchronous belt assembly through respective fixing parts, and are in transmission connection with a synchronous belt in the tray synchronous belt assembly through a synchronous belt pulley in the tray synchronous belt assembly and a corresponding bearing; the encoder is used for cooperating the tray stepping motor to stop the tray module body at the position required to reach.
3. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 2, characterized in that: the tray module body comprises a tray bottom plate, a needle tube supporting block, a reagent strip tray, a reagent strip pressing plate, a reagent strip clamping buckle, a heating plate fixing plate, a magnet assembly power mechanism and a magnet upper and lower optical coupling assembly; the tray bottom plate is erected on the two tray slide rails, and a connecting piece for connecting a synchronous belt in the tray synchronous belt component is fixed on one side of the tray bottom plate; the needle tube supporting block and the reagent strip tray are fixed above the tray bottom plate through corresponding left and right supporting pieces; the needle tube supporting block is correspondingly provided with a plurality of rows of needle head holes for placing three needle heads, each row of needle head holes are positioned on the extension line of the rear end of the open slot corresponding to the needle head hole, the needle tube supporting block is also provided with a plurality of sample holes for placing test tubes containing samples to be detected, each sample hole is also positioned on the extension line of the rear end of the open slot corresponding to the sample hole, and the needle head holes arranged in rows are positioned between the open slot corresponding to the sample hole and the sample hole; a plurality of open slots which are matched with the reagent strips to be placed in are arranged on the reagent strip tray side by side; the reagent strip pressing sheets are distributed on the top surfaces of the reagent strip trays on the two sides of the open slot; the reagent strip clip is positioned on the bottom surface of the reagent strip tray at the front end of the open slot; the heating plate is attached to the bottom surface of the heating plate fixing plate, the heating plate fixing plate is positioned at the tail part of the open slot and fixed below the reagent strip tray, and a plurality of heating lugs are arranged on the top surface of the heating plate fixing plate at intervals upwards; the magnet assembly, the magnet assembly power mechanism and the magnet upper and lower optocoupler assemblies are all positioned between the tray bottom plate and the reagent strip tray, and the magnet assembly is positioned below the heating sheet fixing plate and the heating sheet; the magnet assembly power mechanism is used for lifting or lowering the magnet assembly to complete the step of separating magnetic beads from the reagent strip; and the magnet upper and lower optical coupling component is arranged between the magnet component and the magnet component power mechanism and is used for detecting the position of the magnet component in the tray module body.
4. The full-automatic magnetic bead time-resolved fluorescence immunoassay analyzer of claim 3, wherein: the magnet assembly comprises a magnet fixing seat, a plurality of large magnets, two guide posts and two guide post springs; the bottoms of the two guide posts are fixed on the tray bottom plate, the magnet fixing seat is sleeved on the two guide posts through corresponding linear bearings and can move up and down, and the two guide post springs are respectively sleeved on the two guide posts and abut against the space between the magnet fixing seat and the heating plate fixing plate and are used for assisting the magnet fixing seat to move downwards; all the large magnets are vertically arranged on the magnet fixing seat at intervals and are positioned on the extension line of the rear end of the open slot corresponding to the large magnets, and the top of each large magnet is intersected by two inclined top surfaces along the length direction of the open slot to form a conical surface; correspondingly, a magnet through hole matched with the corresponding large magnet to penetrate is formed in the heating plate fixing plate between the two heating lugs.
5. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 4, wherein: the magnet assembly power mechanism comprises a motor mounting plate, a direct current brushless motor, an eccentric disc, a bearing and a connecting shaft thereof; the motor mounting plate is vertically arranged on the tray bottom plate, and the large surface of the motor mounting plate faces the magnet fixing seat; the direct current brushless motor is transversely fixed on the motor mounting plate, and an output shaft of the direct current brushless motor penetrates through the motor mounting plate and faces the magnet fixing seat; the eccentric disc is connected to an output shaft of the DC brushless motor, the bearing and a connecting shaft thereof are fixed on the disc surface of the eccentric disc, and the fixed position deviates from the output shaft of the DC brushless motor; one side of the magnet fixing seat facing the magnet assembly power mechanism is transversely provided with an adaptive bearing and a long circular hole in which a connecting shaft of the adaptive bearing rolls, and the adaptive bearing and the long circular hole are used for stirring the magnet fixing seat to drive the large magnet to ascend or descend under the driving of the direct current brushless motor.
6. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 1, characterized in that: the liquid transferring and tube withdrawing assembly comprises a liquid transferring and tube withdrawing motor fixing plate, a liquid transferring and tube withdrawing screw motor, a liquid transferring and tube withdrawing guide rail sliding block assembly, a small sliding plate, an injector push plate, a liquid transferring and tube withdrawing motor screw cap, a plurality of puncture needles, an injector fixing plate, a plurality of liquid transferring injectors and an optical coupling sensor; the pipette withdrawing motor fixing plate is transversely and vertically fixed on the front surface of the large sliding plate, the pipette withdrawing screw motor is installed on the pipette withdrawing motor fixing plate, and a screw of the pipette withdrawing screw motor penetrates through the pipette withdrawing motor fixing plate and is vertically arranged downwards; two guide rails in the liquid transferring and tube withdrawing guide rail sliding block assembly are vertically fixed on the front surface of a large sliding plate below a liquid transferring and tube withdrawing motor fixing plate at intervals through screws, the back surface of the small sliding plate is connected with a sliding block in the liquid transferring and tube withdrawing guide rail sliding block assembly, an injector push plate is fixed on the top of the small sliding plate, and a liquid transferring and tube withdrawing motor nut is installed on the injector push plate and matched with a screw rod of a liquid transferring and tube withdrawing screw rod motor; a plurality of puncture needles are vertically fixed on the bottom surface of the small sliding plate at intervals; the syringe fixing plate is positioned below the liquid transferring and tube withdrawing motor fixing plate and is connected with the liquid transferring and tube withdrawing motor fixing plate through two upright posts; a plurality of pipetting injectors are vertically arranged on the top surface of the injector fixing plate at intervals, and a plurality of clamping grooves which are adaptive to the tail parts of the pipetting injectors are formed in the front edge of the injector push plate; the optical coupling sensor is connected to the front surface of a large sliding plate below the pipette withdrawing motor fixing plate through a corresponding fixing piece.
7. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 1, characterized in that: the needle withdrawing head selection assembly comprises a plurality of needle withdrawing head columns, a plurality of needle withdrawing plates, a needle withdrawing head stepping motor, a needle withdrawing head synchronous belt assembly and a needle withdrawing head guide rail sliding block assembly; the lower ends of the two needle withdrawing columns are connected with a needle withdrawing plate, and the front end of the needle withdrawing plate is provided with a notch which is matched with the head needle of the pipetting injector; the needle withdrawing head stepping motor is transversely inwards connected to one side of the small sliding plate through a corresponding fixing piece, an output shaft of the needle withdrawing head stepping motor is connected with one synchronous pulley in the needle withdrawing head synchronous belt assembly through a corresponding bearing, and the other synchronous pulley in the needle withdrawing head synchronous belt assembly is connected to the other side of the small sliding plate through a corresponding bearing and a connecting shaft; guide rails in the needle withdrawing guide rail sliding block assembly are transversely fixed at the lower end of the front face of the small sliding plate through screws, sliding blocks in the needle withdrawing guide rail sliding block assembly are connected with a synchronous belt in the needle withdrawing synchronous belt assembly through corresponding connecting pieces, and the synchronous belt is sleeved on two synchronous belt wheels in the needle withdrawing synchronous belt assembly.
8. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 1, characterized in that: the darkroom door device comprises a darkroom door, two darkroom door guide rail sliding block assemblies and a darkroom door power mechanism; the back of the darkroom door is respectively provided with a groove and a sliding block connecting hole which are matched and embedded into the guide rail sliding block assemblies of the two darkroom doors, the darkroom door is connected to a sliding block in the guide rail sliding block assembly of the darkroom door by passing through the sliding block connecting holes through screws, and the guide rail in the guide rail sliding block assembly of the darkroom door is vertically fixed on the front of the frame through the screws; one side of the darkroom door is connected with a darkroom door power mechanism through a corresponding darkroom door connecting piece; the darkroom door power mechanism consists of a darkroom door stepping motor, the darkroom door connecting piece, a darkroom door synchronous belt component and a darkroom door power mechanism support component; the timing belt in the camera chamber door timing belt assembly is vertically positioned at one side of the camera chamber door, and the camera chamber door connecting piece is connected between the timing belt and the camera chamber door through a screw; at the upper end of the synchronous belt, the darkroom door stepping motor is fixed on the front surface of the frame through a darkroom door stepping motor support in a darkroom door power mechanism support assembly, and a synchronous pulley in the darkroom door synchronous belt assembly is connected to an output shaft of the darkroom door stepping motor through a corresponding bearing; at the lower end of the synchronous belt, a synchronous belt support in the darkroom door power mechanism support assembly is fixed on the front surface of the frame, and the other synchronous belt wheel in the darkroom door synchronous belt assembly is connected to the synchronous belt support through a corresponding bearing and a connecting shaft; the synchronous belt is sleeved on the two synchronous belt wheels.
9. The full-automatic magnetic bead time-resolved fluorescence immunoassay instrument of claim 1, characterized in that: the value reading module consists of a value reading X-axis power mechanism, a value reading Y-axis power mechanism, a lens assembly and a bar code device; the reading X-axis power mechanism and the reading Y-axis power mechanism are respectively used for moving the lens component in the X-axis direction and the Y-axis direction in the horizontal plane, and the reading X-axis power mechanism is also used for moving the lens component in the X-axis direction in the horizontal plane and simultaneously moving the bar code device; the lens assembly is used for carrying out effective light-emitting data acquisition on reagent tubes which are placed on a plurality of reagent strips in the tray module and need to be read, and the bar code device is used for reading bar code information on the plurality of reagent strips;
the value reading X-axis power mechanism comprises a value reading X-axis stepping motor, a value reading X-axis synchronous wheel component, a value reading X-axis guide rail sliding block component and a value reading X-axis fixing plate; the reading X-axis fixing plate is erected on a bottom plate of the rack through two upright posts; the X-axis stepping motor is installed on the front face of a vertical plate of the rack along the Y-axis direction through a corresponding fixing piece, an output shaft of the X-axis stepping motor penetrates through the vertical plate and is connected with one synchronous belt wheel in the value reading X-axis synchronous wheel assembly through a corresponding bearing, and the other synchronous belt wheel in the value reading X-axis synchronous wheel assembly is connected to the back face of the vertical plate of the rack through a corresponding bearing, a connecting shaft and a fixing piece; the guide rail in the reading X-axis guide rail sliding block assembly is fixed on a reading X-axis fixing plate between the two synchronous belt wheels along the X-axis direction through a screw, the bottom surface of one end of a reading Y-axis fixing plate in the reading Y-axis power mechanism is connected with a sliding block in the reading X-axis guide rail sliding block assembly, and the top surface of one end of the reading Y-axis fixing plate is connected with a synchronous belt in the reading X-axis synchronous wheel assembly through a corresponding connecting piece;
the reading Y-axis power mechanism comprises a reading Y-axis stepping motor, a reading Y-axis synchronizing wheel assembly, a reading Y-axis guide rail sliding block assembly, the reading Y-axis fixing plate, a reading Y-axis roller and a reading Y-axis supporting plate; the reading Y-axis supporting plate is also erected on a bottom plate of the rack through two upright posts, and the reading Y-axis roller is arranged at the other end of the reading Y-axis fixing plate; the Y-axis stepping motor is arranged at the roller end of the value reading Y-axis fixing plate along the X-axis direction through a corresponding fixing piece, the output shaft of the Y-axis stepping motor is connected with one synchronous belt wheel in the value reading Y-axis synchronous wheel component through a corresponding bearing, and the other synchronous belt wheel in the value reading Y-axis synchronous wheel component is fixed at the other end of the value reading Y-axis fixing plate through a corresponding bearing, a connecting shaft and a fixing piece; a guide rail in the reading Y-axis guide rail sliding block component is fixed on a reading Y-axis fixing plate between the two synchronous belt wheels along the Y-axis direction through a screw, the lens component is connected with a sliding block in the reading Y-axis guide rail sliding block component, and the lens component is connected with a synchronous belt in the reading Y-axis synchronous wheel component;
the lens assembly comprises a photomultiplier tube, a direct-connection light guide convex seat and a direct-connection light guide concave seat; the photomultiplier is arranged on a directly-connected light guide convex seat, and the directly-connected light guide convex seat is arranged on a directly-connected light guide concave seat; the direct-connection light guide convex seat is connected with a synchronous belt in the reading Y-axis synchronous wheel component, and the direct-connection light guide concave seat is connected with a sliding block in the reading Y-axis guide rail sliding block component;
the bar code device is fixed on the reading Y-axis fixing plate through corresponding fixing parts.
CN201811016234.XA 2018-09-02 2018-09-02 Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance Active CN109142708B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201811016234.XA CN109142708B (en) 2018-09-02 2018-09-02 Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance
PCT/CN2019/078574 WO2020042596A1 (en) 2018-09-02 2019-03-19 Fully-automatic magnetic bead time-resolved fluoroimmunoassay instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811016234.XA CN109142708B (en) 2018-09-02 2018-09-02 Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance

Publications (2)

Publication Number Publication Date
CN109142708A CN109142708A (en) 2019-01-04
CN109142708B true CN109142708B (en) 2023-01-24

Family

ID=64826129

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811016234.XA Active CN109142708B (en) 2018-09-02 2018-09-02 Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance

Country Status (2)

Country Link
CN (1) CN109142708B (en)
WO (1) WO2020042596A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109752537A (en) * 2019-01-25 2019-05-14 武汉璟泓万方堂医药科技股份有限公司 New chemical shines-immune chromatography test paper and its detection method
JP2022520532A (en) * 2019-03-12 2022-03-31 陳輝 Nucleic acid extractor
CN110045134B (en) * 2019-05-10 2023-08-29 苏州仁端生物医药科技有限公司 Full-automatic chemiluminescence immunoassay instrument and use method thereof
TWI756585B (en) * 2019-11-18 2022-03-01 瑞基海洋生物科技股份有限公司 Biochemical reactor, stirring device and extraction method thereof
CN113495087B (en) * 2020-04-03 2023-04-07 深圳市帝迈生物技术有限公司 POCT blood cell analyzer and detection method thereof
CN112391275A (en) * 2020-11-08 2021-02-23 新羿制造科技（北京）有限公司 Micro-droplet chip crimping device
CN113567696A (en) * 2021-07-26 2021-10-29 四川新健康成生物股份有限公司 Full-automatic chemiluminescence immunoassay device
CN113670900A (en) * 2021-09-07 2021-11-19 苏州翊讯生物科技有限公司 Multichannel chemiluminescence immunoassay analyzer
CN114113048B (en) * 2021-11-23 2023-10-27 南京晶捷生物科技有限公司 Optical detection equipment
CN116773841A (en) * 2022-03-08 2023-09-19 上海萨迦生物科技有限公司 Full-automatic immunity analyzer based on laser confocal technology and detection method thereof
CN115304003B (en) * 2022-08-23 2023-10-27 楚天科技股份有限公司 Magnetic microbead coating machine
CN115505491B (en) * 2022-09-23 2023-09-05 广州湾区生物科技有限公司 Reagent strip subassembly, reagent processing structure and nucleic acid extraction appearance

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0185330A2 (en) * 1984-12-18 1986-06-25 Cetus Corporation Multi-sample liquid handling system
CN103551216A (en) * 2013-11-06 2014-02-05 北京自由度科学机器有限公司 Full automatic liquid relief instrument and application thereof
CN103551217A (en) * 2013-11-06 2014-02-05 北京自由度科学机器有限公司 Liquid transfer mechanism for full-automatic liquid transfer instrument and application thereof
CN203490229U (en) * 2013-02-04 2014-03-19 石家庄康普生科技有限公司 Quasi-automatic plate type immune analyzer
CN105628687A (en) * 2016-02-24 2016-06-01 南京诺尔曼生物技术有限公司 Full-automatic chemiluminiscence tester
CN205991944U (en) * 2016-08-26 2017-03-01 深圳市库贝尔生物科技股份有限公司 A kind of chemical luminescence detector
WO2017128806A1 (en) * 2016-01-27 2017-08-03 广州万孚生物技术股份有限公司 Automatic fluorescence quantitative immunoassay analyzer and detection method
CN206450686U (en) * 2017-02-25 2017-08-29 广州源起健康科技有限公司 A kind of composite reagent bar
CN107219352A (en) * 2017-06-27 2017-09-29 广州源起健康科技有限公司 A kind of reagent strip pusher of chemical illumination immunity analysis instrument
CN107328925A (en) * 2017-08-15 2017-11-07 广州源起健康科技有限公司 A kind of darkroom detection means of chemical illumination immunity analysis instrument
CN107389915A (en) * 2017-08-15 2017-11-24 广州源起健康科技有限公司 A kind of Full-automatic chemiluminescence immunoassay analysis meter
CN107843737A (en) * 2017-12-08 2018-03-27 均强机械(苏州)有限公司 Full-automatic micro-fluidic chemiluminescence immunoassay detector
CN107918027A (en) * 2017-11-17 2018-04-17 南通伊仕生物技术股份有限公司 A kind of small size automatic chemiluminescent analyzer
CN108333357A (en) * 2018-02-09 2018-07-27 广州源起健康科技有限公司 A kind of magnetic bead time-resolved fluoroimmunoassay quantitatively detects CK-MB kits

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7842504B2 (en) * 2004-04-02 2010-11-30 Siemens Healthcare Diagnostics Inc. Method for increasing throughput in an automatic clinical analyzer by duplicating reagent resources
EP3096149A4 (en) * 2014-01-16 2017-10-18 Universal Bio Research Co., Ltd. Soft stopper-penetrating pipetting device, and soft stopper-penetrating pipetting method
US10620226B2 (en) * 2014-07-28 2020-04-14 Douglas Scientific, LLC Instrument for analyzing biological samples and reagents
CN204269661U (en) * 2014-10-28 2015-04-15 利多(香港)有限公司 The sampling apparatus of full-automatic Hematometer
CN107356587B (en) * 2017-08-24 2024-03-01 北京贝泰科技有限公司 Light-activated chemiluminescence instant detection system
CN108414737A (en) * 2018-06-13 2018-08-17 安徽为臻生物工程技术有限公司 A kind of Full-automatic chemiluminescence immunoassay analysis meter

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0185330A2 (en) * 1984-12-18 1986-06-25 Cetus Corporation Multi-sample liquid handling system
CN203490229U (en) * 2013-02-04 2014-03-19 石家庄康普生科技有限公司 Quasi-automatic plate type immune analyzer
CN103551216A (en) * 2013-11-06 2014-02-05 北京自由度科学机器有限公司 Full automatic liquid relief instrument and application thereof
CN103551217A (en) * 2013-11-06 2014-02-05 北京自由度科学机器有限公司 Liquid transfer mechanism for full-automatic liquid transfer instrument and application thereof
WO2017128806A1 (en) * 2016-01-27 2017-08-03 广州万孚生物技术股份有限公司 Automatic fluorescence quantitative immunoassay analyzer and detection method
CN105628687A (en) * 2016-02-24 2016-06-01 南京诺尔曼生物技术有限公司 Full-automatic chemiluminiscence tester
CN205991944U (en) * 2016-08-26 2017-03-01 深圳市库贝尔生物科技股份有限公司 A kind of chemical luminescence detector
CN206450686U (en) * 2017-02-25 2017-08-29 广州源起健康科技有限公司 A kind of composite reagent bar
CN107219352A (en) * 2017-06-27 2017-09-29 广州源起健康科技有限公司 A kind of reagent strip pusher of chemical illumination immunity analysis instrument
CN107328925A (en) * 2017-08-15 2017-11-07 广州源起健康科技有限公司 A kind of darkroom detection means of chemical illumination immunity analysis instrument
CN107389915A (en) * 2017-08-15 2017-11-24 广州源起健康科技有限公司 A kind of Full-automatic chemiluminescence immunoassay analysis meter
CN107918027A (en) * 2017-11-17 2018-04-17 南通伊仕生物技术股份有限公司 A kind of small size automatic chemiluminescent analyzer
CN107843737A (en) * 2017-12-08 2018-03-27 均强机械(苏州)有限公司 Full-automatic micro-fluidic chemiluminescence immunoassay detector
CN108333357A (en) * 2018-02-09 2018-07-27 广州源起健康科技有限公司 A kind of magnetic bead time-resolved fluoroimmunoassay quantitatively detects CK-MB kits

Also Published As

Publication number Publication date
CN109142708A (en) 2019-01-04
WO2020042596A1 (en) 2020-03-05

Similar Documents

Publication Publication Date Title
CN109142708B (en) Full-automatic magnetic bead time-resolved fluorescence immunoassay appearance
CN107389915B (en) Full-automatic chemiluminescence immunoassay instrument
US11693019B2 (en) Automated liquid-phase immunoassay apparatus
JP6385944B2 (en) Chemiluminescence measuring apparatus and method
KR101833297B1 (en) Assay apparatuses, methods and reagents
CN110045134B (en) Full-automatic chemiluminescence immunoassay instrument and use method thereof
US8382968B2 (en) Conductor/insulator/porous film-device and its use with the electrochemiluminescence-based analytical methods
CN113125787A (en) Full-automatic chemiluminescence immunoassay analyzer
CN111007239B (en) Homogeneous immunoassay method based on ortho-position touch effect and acridine ester chemiluminescence quenched by graphene oxide and using equipment
CN112534260A (en) Automatic liquid phase immune reaction analysis device and method thereof
CN210293858U (en) Magnetic separation cleaning device
CN111948415A (en) Parallel detection heterogeneous chemiluminescence immunoassay analyzer, multi-tube parallel reagent strip and control method
CN108107227A (en) A kind of chemiluminescence immune assay chip cartridges and operating method
CN214374309U (en) Chemiluminescence immunoassay appearance
CN113567695A (en) Full-automatic chemiluminescence immunoassay equipment
CN113308366A (en) Full-automatic nucleic acid extraction device and method
CN109142709B (en) Puncture pipetting device of time-resolved fluoroimmunoassay analyzer
CN111323411B (en) Multi-project combined chemiluminescent instant detection system
CN108362901B (en) Chemiluminescence immunoassay device and method
CN112683800A (en) Homogeneous phase chemiluminescence analyzer, chip box and detection method thereof
CN204086274U (en) A kind of time-resolved fluorescence immunoassay instrument
CN114636833A (en) Full-automatic chemiluminescence immunoassay analyzer
CN210015117U (en) Automatic sample adding machine
CN113567696A (en) Full-automatic chemiluminescence immunoassay device
CN214794844U (en) Full-automatic chemiluminescence immunoassay analyzer

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant