CN112683800A - Homogeneous phase chemiluminescence analyzer, chip box and detection method thereof - Google Patents
Homogeneous phase chemiluminescence analyzer, chip box and detection method thereof Download PDFInfo
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- CN112683800A CN112683800A CN202011639214.5A CN202011639214A CN112683800A CN 112683800 A CN112683800 A CN 112683800A CN 202011639214 A CN202011639214 A CN 202011639214A CN 112683800 A CN112683800 A CN 112683800A
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Abstract
The invention relates to the technical field of chemiluminescence immunity, and provides a homogeneous phase chemiluminescence analyzer, a chip box and a detection method thereof, wherein the homogeneous phase chemiluminescence analyzer comprises a chip box code scanning module, a puncture mechanism and a detection mechanism which are arranged on the same straight line; the volume of the whole analyzer can be further reduced by arranging the two analyzers on the same straight line; the chip box code scanning module is used for scanning the chip box to acquire user detection project information; the detection mechanism is a photon counting device and is used for carrying out photon counting on weak light emitted from the reaction cup so as to obtain a concentration value of the object to be detected through counting. The puncturing mechanism is used for puncturing a sealing film on the chip box so as to sample the chip box or take samples. Meanwhile, a chip box is provided, reaction solution is packaged in the chip box, and a reaction cup is arranged, so that reaction can be directly finished in the chip box; meanwhile, the method also comprises a step flow of a detection method of the homogeneous phase chemiluminescence analyzer.
Description
Technical Field
The application relates to the technical field of chemiluminescence immunity, and relates to a homogeneous phase chemiluminescence analyzer.
Background
The chemiluminescence immunoassay technique is a detection technique with high sensitivity and high specificity in the field of in vitro diagnosis, and is often used for detecting tumor markers, hormones, vitamins and other substances with extremely low content in human blood.
The chemiluminescence immunoassay technology is divided into homogeneous chemiluminescence and heterogeneous chemiluminescence according to different reaction forms. Most of the chemiluminescence apparatuses on the market at present are heterogeneous methods, and the heterogeneous methods have the defects that a reaction system needs to be separated, and antigen substances which are not combined are separated. The homogeneous phase chemiluminescence technology does not need to separate a reaction system, all immunoreactions are completed in a solution environment, the operation is simple, washing is not needed, the quantitative accuracy is improved, and the experimental repeatability is good.
Therefore, how to provide a miniaturized single-person homogeneous chemiluminescence analyzer is a problem to be solved.
Disclosure of Invention
In order to solve the problems in the prior art, a miniaturized single-person homogeneous phase chemiluminescence analyzer is provided.
The invention provides a homogeneous phase chemiluminescence analyzer, which comprises:
the chip box code scanning module, the puncture mechanism and the detection mechanism are arranged on the same straight line;
the chip box code scanning module is used for scanning the chip box to acquire user detection project information;
the detection mechanism is a photon counting device and is used for carrying out photon counting on weak light emitted from the reaction cup so as to obtain a concentration value of the object to be detected through counting.
The puncturing mechanism is used for puncturing a sealing film on the chip box so as to sample the chip box or take samples.
Specifically, the sample injection mechanism is arranged in parallel with a straight line where the puncture mechanism is located;
the sample adding mechanism comprises a sample frame driving motor module which is used for driving the sample frame to move to a sampling position so as to sample the sample frame.
Specifically, the sampling mechanism further comprises a sample code scanning module, and the sample code scanning module is used for scanning and identifying the identification code on the sample and feeding back identification information to the control end.
Specifically, the sample adding structure is arranged above the side penetrating mechanism and is perpendicular to a straight line where the penetrating mechanism is located, and the sample adding structure is used for adding samples or sampling samples to the chip box.
Specifically, the sample adding structure comprises a liquid level detection module;
the liquid level detection module controls the sample needle to move to a designated position of the test tube and to enter the depth of the test tube for sucking the sample.
Specifically, the application of sample structure still includes application of sample arm X axle driving motor, application of sample arm Z axle driving motor for drive application of sample arm in the motion of level and vertical direction.
Specifically, the temperature control module is arranged on the same line with the puncture mechanism and is used for carrying out incubation on the reagent on the chip box.
Specifically, the reagent box driving motor module and the puncture mechanism are arranged on the same straight line and used for driving the chip box to move.
The invention provides a chip box applied to a homogeneous phase chemiluminescence analyzer, which comprises
Reagent cups, reaction cups and drive channels;
the reagent cup is used for loading reagent solution;
the reaction cup is used as a reaction vessel for samples and reagents;
the driving groove is used for being driven by the driving mechanism so as to realize the movement of the chip box.
The invention provides a detection method of a homogeneous phase chemiluminescence analyzer, which comprises the following steps:
confirming that the chip box and the sample are put in place, scanning and confirming the chip box and the sample, and feeding code scanning information back to the control end;
conveying the chip box to an incubation puncture position for incubation and puncturing the chip box at the same time;
the sample rack driving motor moves the sample rack to a sampling position for sampling by the sample needle;
and conveying the chip box to a mixing structure, and sequentially carrying out sample adding, incubation and mixing actions on the chip box and the first reagent and the second reagent.
And conveying the chip box to an optical detection position for detection according to the set reaction time.
The invention provides a homogeneous phase chemiluminescence analyzer, a chip box and a detection method thereof, wherein the homogeneous phase chemiluminescence analyzer comprises a chip box code scanning module, a puncture mechanism and a detection mechanism which are arranged on the same straight line; the volume of the whole analyzer can be further reduced by arranging the two analyzers on the same straight line; the chip box code scanning module is used for scanning the chip box to acquire user detection project information; the detection mechanism is a photon counting device and is used for carrying out photon counting on weak light emitted from the reaction cup so as to obtain a concentration value of the object to be detected through counting. The puncturing mechanism is used for puncturing a sealing film on the chip box so as to sample the chip box or take samples. Meanwhile, a chip box is provided, reaction solution is packaged in the chip box, and a reaction cup is arranged, so that reaction can be directly finished in the chip box; meanwhile, the method also comprises a step flow of a detection method of the homogeneous phase chemiluminescence analyzer.
Drawings
Fig. 1 is a schematic diagram illustrating an overall structure of a homogeneous chemiluminescence analyzer according to an embodiment;
FIG. 2 is a schematic diagram of a sample injection mechanism of a homogeneous chemiluminescence analyzer according to an embodiment;
FIG. 3 is a schematic diagram of a chip cassette feed channel of a homogeneous chemiluminescence analyzer according to an embodiment;
fig. 4 is a schematic structural diagram of a sample application mechanism of a homogeneous chemiluminescence analyzer according to an embodiment;
FIG. 5 is a schematic structural diagram of a homogeneous phase chemiluminescence analyzer chip cartridge provided by an embodiment;
fig. 6 is a wire frame diagram of a detection method of a homogeneous chemiluminescence analyzer according to an embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present disclosure more clearly understood, the present disclosure is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the scope of the invention.
In the description of the present disclosure, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present disclosure and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be configured and operated in a specific orientation, and thus, should not be construed as limiting the present disclosure.
Furthermore, in the description of the present disclosure, unless explicitly stated or limited otherwise, the terms "mounted," "connected," and the like are to be construed broadly and can include, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, or may be communicated with each other inside the two elements, or may be wirelessly connected or wired connected. The specific meaning of the above terms in the context of the present invention may be understood in a specific context to those of ordinary skill in the art.
Furthermore, the technical features mentioned in the different embodiments of the present disclosure described later can be combined with each other as long as they do not conflict with each other.
In order to achieve the purpose, the invention adopts the following scheme:
as shown in fig. 1 and 3, a chip cartridge feeding channel 01, a sample holder unit 02, a sample loading arm unit 03, a control circuit board 04, a photon counter 11, a screw motor 12, a code scanner 13, a reagent kit detection sensor 14, a metal groove 15, an upper cover plate 16, a driving motor 17, a puncture module 18, a side plate 19, and a puncture motor 10.
The invention provides a homogeneous phase chemiluminescence analyzer, which comprises:
the chip box code scanning module 13, the puncture mechanism 18 and the detection mechanism are arranged on the same straight line;
the chip box code scanning module is used for scanning the chip box to acquire user detection project information;
the detection mechanism is a photon counting device and is used for carrying out photon counting on weak light emitted from the reaction cup so as to obtain a concentration value of the object to be detected through counting.
The puncturing mechanism is used for puncturing a sealing film on the chip box so as to sample the chip box or take samples.
In one embodiment, a homogeneous chemiluminescent analyzer is provided comprising: a code scanner 13, a puncture module 18 and a photon counter 11 which are arranged on the same straight line.
In another embodiment, the structure of the chip box feeding channel comprises a photon counter 11, a screw motor 12, a code scanner 13, a reagent box detection sensor 14, a metal groove 15, an upper cover plate 16, a driving motor 17, a puncture module 18, a side plate 19 and a puncture motor 10.
Description of technical effects: the invention provides a homogeneous phase chemiluminescence analyzer, a chip box and a detection method thereof, wherein the homogeneous phase chemiluminescence analyzer comprises a chip box code scanning module, a puncture mechanism and a detection mechanism which are arranged on the same straight line; the volume of the whole analyzer can be further reduced by arranging the two analyzers on the same straight line; the chip box code scanning module is used for scanning the chip box to acquire user detection project information; the detection mechanism is a photon counting device and is used for carrying out photon counting on weak light emitted from the reaction cup so as to obtain a concentration value of the object to be detected through counting. The puncturing mechanism is used for puncturing a sealing film on the chip box so as to sample the chip box or take samples. Meanwhile, a chip box is provided, reaction solution is packaged in the chip box, and a reaction cup is arranged, so that reaction can be directly finished in the chip box; meanwhile, the method also comprises a step flow of a detection method of the homogeneous phase chemiluminescence analyzer.
Specifically, the sample injection mechanism is arranged in parallel with a straight line where the puncture mechanism is located;
the sample adding mechanism comprises a sample frame driving motor module which is used for driving the sample frame to move to a sampling position so as to sample the sample frame.
In one embodiment, the sample feeding mechanism 2 includes a connection plate 21, a sample rack 22, a test tube 23, a test tube detection sensor 24, a base 25, and a driving motor 26.
Specifically, the sampling mechanism further comprises a sample code scanning module, and the sample code scanning module is used for scanning and identifying the identification code on the sample and feeding back identification information to the control end.
According to one embodiment, the sample adding mechanism 3 includes a sample adding needle 31, a liquid level detection module 32, a cleaning liquid preheating module 33, a sample adding needle Z-axis motor 34, a flexible flat cable 35, a beam 36, a sample adding needle X-axis motor 37, and a support base 38.
Specifically, the sample adding structure is arranged above the side penetrating mechanism and is perpendicular to a straight line where the penetrating mechanism is located, and the sample adding structure is used for adding samples or sampling samples to the chip box.
Specifically, the sample adding structure comprises a liquid level detection module;
the liquid level detection module controls the sample needle to move to a designated position of the test tube and to enter the depth of the test tube for sucking the sample.
Specifically, the application of sample structure still includes application of sample arm X axle driving motor, application of sample arm Z axle driving motor for drive application of sample arm in the motion of level and vertical direction.
Specifically, the temperature control module is arranged on the same line with the puncture mechanism and is used for carrying out incubation on the reagent on the chip box.
Specifically, the reagent box driving motor module and the puncture mechanism are arranged on the same straight line and used for driving the chip box to move.
The invention provides a chip box applied to a homogeneous phase chemiluminescence analyzer, which comprises
Reagent cups, reaction cups and drive channels;
the reagent cup is used for loading reagent solution;
the reaction cup is used as a reaction vessel for samples and reagents;
the driving groove is used for being driven by the driving mechanism so as to realize the movement of the chip box.
In one embodiment, the chip case comprises a reagent cup 51, a reaction cup 52, and a driving groove 53, wherein the driving groove 53 comprises a horizontal plate 533, a vertical plate 532, and a step 531; a light shield inner ring 55, a light shield outer ring 56, a notch 54, a flat plate 57, an integral support plate 59, and a reinforcing device 58.
The invention provides a detection method of a homogeneous phase chemiluminescence analyzer, which comprises the following steps:
s1, confirming that the chip box and the sample rack are in place, scanning and confirming the chip box and the sample rack, and feeding code scanning information back to the control end;
s2, conveying the chip box to an incubation puncture position for incubation, and puncturing the chip box at the same time;
s3, the sample rack is moved to the sampling position by the sample rack driving motor and used for sampling by the sample needle;
and S4, conveying the chip box to a mixing structure, and sequentially carrying out sample adding, incubation and mixing actions on the chip box and the first reagent and the second reagent.
S5, the chip box is transported to the optical detection position for detection according to the set reaction time.
The present invention is not intended to be limited to the particular embodiments shown, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A homogeneous chemiluminescent analyzer comprising:
the chip box code scanning module, the puncture mechanism and the detection mechanism are arranged on the same straight line;
the chip box code scanning module is used for scanning the chip box to acquire user detection project information;
the detection mechanism is a photon counting device and is used for carrying out photon counting on weak light emitted from the reaction cup so as to obtain a concentration value of the object to be detected through counting.
The puncturing mechanism is used for puncturing a sealing film on the chip box so as to sample the chip box or take samples.
2. The analyzer of claim 1, comprising:
the sample injection mechanism is arranged in parallel with the straight line where the puncture mechanism is located;
the sample adding mechanism comprises a sample frame driving motor module which is used for driving the sample frame to move to a sampling position so as to sample the sample frame.
3. The analyzer of claim 2, comprising:
the sample feeding mechanism further comprises a sample scanning module for scanning and identifying the identification code on the sample and feeding back the identification information to the control end.
4. The analyzer of claim 1, comprising:
and the sample adding structure is arranged above the side penetrating mechanism and is vertical to the straight line where the puncturing mechanism is positioned, and the sample adding structure is used for adding samples or sampling the chip box.
5. The analyzer of claim 4, comprising:
the sample adding structure comprises a liquid level detection module;
the liquid level detection module controls the sample needle to move to a designated position of the test tube and to enter the depth of the test tube for sucking the sample.
6. The analyzer of claim 4, comprising:
the sample adding structure further comprises a sample adding arm X-axis driving motor and a sample adding arm Z-axis driving motor, and the sample adding arm X-axis driving motor and the sample adding arm Z-axis driving motor are used for driving the sample adding arm to move in the horizontal direction and the vertical direction.
7. The analyzer of claim 1, comprising:
and the temperature control module is arranged on the same line with the puncture mechanism and is used for carrying out incubation on the reagent by the chip box.
8. The analyzer of claim 1, comprising:
and the kit driving motor module and the puncture mechanism are arranged on the same straight line and are used for driving the chip box to move.
9. A chip cartridge for a homogeneous chemiluminescent analyzer, comprising
Reagent cups, reaction cups and drive channels;
the reagent cup is used for loading reagent solution;
the reaction cup is used as a reaction vessel for samples and reagents;
the driving groove is used for being driven by the driving mechanism so as to realize the movement of the chip box.
10. A detection method of a homogeneous phase chemiluminescence analyzer is characterized by comprising the following steps:
confirming that the chip box and the sample rack are in place, scanning and confirming the chip box and the sample rack, and feeding code scanning information back to the control end;
conveying the chip box to an incubation puncture position for incubation and puncturing the chip box at the same time;
the sample rack driving motor moves the sample rack to a sampling position for sampling by the sample needle;
and conveying the chip box to a mixing structure, and sequentially carrying out sample adding, incubation and mixing actions on the chip box and the first reagent and the second reagent.
And conveying the chip box to an optical detection position for detection according to the set reaction time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011639214.5A CN112683800A (en) | 2020-12-31 | 2020-12-31 | Homogeneous phase chemiluminescence analyzer, chip box and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011639214.5A CN112683800A (en) | 2020-12-31 | 2020-12-31 | Homogeneous phase chemiluminescence analyzer, chip box and detection method thereof |
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| CN112683800A true CN112683800A (en) | 2021-04-20 |
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| CN202011639214.5A Pending CN112683800A (en) | 2020-12-31 | 2020-12-31 | Homogeneous phase chemiluminescence analyzer, chip box and detection method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820513A (en) * | 2021-09-18 | 2021-12-21 | 浙江盛域医疗技术有限公司 | Arm group module for sample adding |
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|---|---|---|---|---|
| US5736320A (en) * | 1993-05-12 | 1998-04-07 | Schlederer; Thomas | Method of detecting substances by chemiluminescence |
| CN101059508A (en) * | 2007-05-10 | 2007-10-24 | 上海交通大学 | Homogeneous immune analysis nano device construction method |
| CN108107227A (en) * | 2018-02-09 | 2018-06-01 | 深圳市活水床旁诊断仪器有限公司 | A kind of chemiluminescence immune assay chip cartridges and operating method |
| CN111721955A (en) * | 2019-03-21 | 2020-09-29 | 深圳市活水床旁诊断仪器有限公司 | Chemiluminescence immunity analyzer and detection method |
-
2020
- 2020-12-31 CN CN202011639214.5A patent/CN112683800A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5736320A (en) * | 1993-05-12 | 1998-04-07 | Schlederer; Thomas | Method of detecting substances by chemiluminescence |
| CN101059508A (en) * | 2007-05-10 | 2007-10-24 | 上海交通大学 | Homogeneous immune analysis nano device construction method |
| CN108107227A (en) * | 2018-02-09 | 2018-06-01 | 深圳市活水床旁诊断仪器有限公司 | A kind of chemiluminescence immune assay chip cartridges and operating method |
| CN111721955A (en) * | 2019-03-21 | 2020-09-29 | 深圳市活水床旁诊断仪器有限公司 | Chemiluminescence immunity analyzer and detection method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820513A (en) * | 2021-09-18 | 2021-12-21 | 浙江盛域医疗技术有限公司 | Arm group module for sample adding |
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Application publication date: 20210420 |
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