CN109142708A

CN109142708A - A kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument

Info

Publication number: CN109142708A
Application number: CN201811016234.XA
Authority: CN
Inventors: 李根平
Original assignee: Guangzhou Origin Health Technology Co Ltd
Current assignee: Guangzhou Origin Health Technology Co Ltd
Priority date: 2018-09-02
Filing date: 2018-09-02
Publication date: 2019-01-04
Anticipated expiration: 2038-09-02
Also published as: CN109142708B; WO2020042596A1

Abstract

The invention discloses a kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, instrument is divided into interior room and mistress by rack；Electronic component box is fixed on interior room upper layer, and darkroom shell is located at interior room lower layer, and darkroom door gear is arranged on the rack, and cooperation darkroom shell forms the openable and closable darkroom space in side；Tray mold group is located at mistress lower layer, can place multiple reagent strips, and is passed to interior room lower layer or retreats to mistress lower layer；It punctures liquid relief mould group and is located at mistress upper layer, the film and automatic sucking or the liquid being discharged inside its of reagent strip top surface can be pierced through automatically；Barcode scanning readings mould group is located at interior room lower layer and in darkroom interior of shell, for excite and read be incubated for after reagent strip fluorescence information；Pass through the rational deployment of interior mistress, in conjunction with the design of the compact science to correlation function mould group, not only structure it is simpler it is compact, cost is less expensive, but also improves the degree of automation of instrument entirety, the medical institutions for being very suitable to China use, popularize.

Description

A kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument

Technical field

The present invention relates to industries institute's used times such as in-vitro diagnosis and biomedicine, animal epidemic, food safety and drug screening Between resolved fluorometric immunoassay instrument field, more particularly to it is a kind of simple and compact for structure, high degree of automation full-automatic Magnetic bead time-resolved fluorescence immunoassay instrument.

Background technique

POCT real-time test (Point of Care Testing) is in-vitro diagnosis industry labelled immune skill in recent years The important development direction of art and fastest-rising branch.Technology involved in POCT is varied, in brief, Ke Yifen Simply to develop the color, enzyme label, immunoassay, optically and electrically biosensor, Electrochemical Detection, spectrophotometric and biochip Deng.

Immuno-labelling technique undergoes over one hundred year developing history, can be by immuno-labelling technique according to the difference of marker Detection method is divided into: 1, radiommunoassay, immunoradiometric assay (RIA, IRMA)；2, fluoroimmunoassay (FIA)；3, enzyme is exempted from Epidemic disease analyzes (EIA)；4, golden labelling immunoassay；5, bioluminescence immunoassay (BIA)；6, enzyme-fluoroimmunoassay (E- CIA)；7, chemiluminescence immune assay (CLIA)；8, time-resolved fluoroimmunoassay (TRFIA)；9, electrochemical luminescence is immune It analyzes (ECLIA).

Time-resolved fluoroimmunoassay (TRFIA), using lanthanide chelate europium, terbium, samarium, dysprosium as marker, tool There are wider excitation spectrum, relatively narrow emission spectrum, advantageously reduce background, improves sensitivity；Ultraviolet excitation has higher amount Sub- yield, larger Stokes displacement, the spectrum for avoiding excitation spectrum and fluorescence emission spectrum and bio-matrix from emitting is overlapped, glimmering The advantages that light decay time is long, it is more wider than conventional fluorescent substance detection range, specific more preferable.

And magnetic bead time-resolved fluoroimmunoassay is based on immunomagnetic beads using rare earth ion chelate as marker Time-resolved fluoroimmunoassay, be that a kind of highly sensitive, special, stabilizations, "dead" pollution, detection are repeated Well, the immunologic detection method that standard curve range is wide, has a wide range of application, the isotope for overcoming radiommunoassay (RIA) are dirty Dye, the disadvantages of half-life short and EIA enzyme immunoassay (EIA) stability are poor, while it being able to carry out the joint-detection of many kinds of substance, extensively It is general be applied to biomedical research and clinical medical inspection, it is considered to be a most promising trace detection means it One.

The magnetic bead used in magnetic bead time-resolved fluoroimmunoassay refers to immune magnetic microsphere (IMMS) or is immunized Magnetic bead (IMB) is immunology and Superparamagnetic beads in conjunction with and a kind of new material for growing up.Immunomagnetic beads are to be coated with Antibody or superparamagnetism microballoon with antibody binding function can be with targets when it mixes incubation with the sample containing target substance Substance specifically in conjunction with and form the compound with magnetic response, this compound can be detained by magnetic field, thus with its in sample He separates impurity.Immunomagnetic bead technique has high separation purity, reservation target substance activity, high sensitivity, specificity height, detection speed The advantages that degree is fast, less toxic, reproducible, easy to operate and does not need expensive instrument and equipment.

Superparamagnetism microballoon by chemical group modification can pass through covalent coupling as coating medium, antigen or antibody Onto magnetic bead, the physisorption than the microwell plate that polystyrene is material is stronger；The surface area of magnetic bead is bigger, can combine More protein moleculars, opposite plate absorption greatly reduce antibody loss；Realize that immune separation and enrichment combine together；As one kind Small sized flow phase carrier can make reaction quickly reach dynamic equilibrium, to accelerate reaction speed；A variety of couplings are integrated to be directed to The not magnetic bead of the antibody of synantigen makes it possible to detect determinand different in same sample；Without being coated with polystyrene board (solid phase carrier), reagent cost is low, and the quality of production is controllable.

The immune complex formed using magnetic separation technique Direct precipitation in externally-applied magnetic field, being not required to centrifugation can will exempt from Epidemic disease compound is mutually separated with unbonded material.Due to magnetic bead has bigger bonded area and can disperse in the liquid phase sufficiently instead It answers, expands detection range, shorten the reaction time, substantially increase sensitivity.Since magnetic bead and antigen or antibody are covalent Coupling overcomes the unstability of physical absorption, therefore the time that immunomagnetic beads save is also more long and more stable.

But Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in the research and development side of China in recent years Still in its infancy, how independent research one kind is simple and compact for structure, Full-automatic magnetic beads of high degree of automation for the development in face Time-resolved fluorescence immunoassay instrument, it has also become the in-vitro diagnosis research direction one of important with biomedical industry.

Summary of the invention

In order to solve the above technical problems, the present invention provides a kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, knot Structure it is simpler it is compact, cost is less expensive, and high degree of automation, it is easy to operate quickly.

Technical scheme is as follows: a kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, comprising: rack, Tray mold group punctures liquid relief mould group, darkroom shell, darkroom door gear and barcode scanning readings mould group；Wherein: rack divides entire instrument At two parts of interior room and mistress；The top area of interior room is fixed with electronic component box, and darkroom shell is located at the lower layer of interior room Region, darkroom door gear are arranged on the rack, for cooperating darkroom shell to form the openable and closable darkroom space in side；Tray mold group Positioned at the lower region of mistress, for placing and fixing multiple reagent strips to be detected, and reagent strip is sent into interior room Layer region is exited to the lower region of mistress；The top area that liquid relief mould group is located at mistress is punctured, for piercing through reagent automatically It is covered on the film of Reagent Tube top surface on item and automatic sucking or its internal liquid is discharged；Barcode scanning readings mould group is located at interior The lower region of room, and be in darkroom shell inside, for excite and read be incubated for after reagent strip fluorescence information.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the tray mold group is moved by tray mold group Force mechanisms and tray mold group ontology composition, the tray mold group ontology are mounted on tray mold group power mechanism, the tray mold Group power mechanism be used for by the tray mold group ontology for being installed with multiple reagent strips side by side be sent into the lower region of interior room or exit to The lower region of mistress；The tray mold group power mechanism includes pallet stepper motor, two pallet sliding rails, encoder and pallet Synchronous belt component；The pallet synchronous belt component is located therein the top of a pallet sliding rail, and the tray mold group ontology is set up On two pallet sliding rails, and the side of the tray mold group ontology is via in corresponding connector and pallet synchronous belt component Synchronous belt is connected；The pallet stepper motor and encoder are located at the both ends of pallet synchronous belt component, and via respective Fixing piece be fixed on the pallet sliding rail both ends below pallet synchronous belt component, and via the synchronous belt in pallet synchronous belt component Wheel and corresponding bearing are connect with the toothed belt transmission in pallet synchronous belt component；The encoder is for cooperating pallet stepping electric Tray mold group ontology is rested on the position for needing to reach by machine.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the tray mold group ontology includes pallet Bottom plate, needle tubing tray, reagent strip pallet, reagent strip tabletting, reagent strip clip, heating sheet, heating sheet fixed plate, magnet assemblies and Optocoupler assembly above and below magnet assemblies power mechanism and magnet；The tray substrate is erected on two pallet sliding rails, in the pallet The side of bottom plate is fixed with the connector for being connected with the synchronous belt in pallet synchronous belt component；The needle tubing tray and examination Agent bar pallet is fixed on the top of tray substrate via corresponding left and right supporting element；It is correspondingly arranged on the needle tubing tray more Row for placing the syringe needle holes of three syringe needles, every row's syringe needle hole be respectively positioned on its corresponding to open slot rear end extended line on, Multiple sample wells are additionally provided on the needle tubing tray, for placing the test tube that sample to be tested is housed, each sample well is also equal Positioned at its corresponding to open slot rear end extended line on, and the syringe needle hole set in a row be located at corresponding open slot and Between sample well；Being arranged side by side on the reagent strip pallet has multiple open slots for being adapted to and being put into reagent strip；The reagent strip pressure Piece is distributed on the top surface of reagent strip pallet of open slot two sides；The reagent strip clip is located at the reagent strip holder of opening front of the slot On the bottom surface of disk；The heating sheet is mounted on the bottom surface of heating sheet fixed plate, and the heating sheet fixed plate is located at the tail of open slot Portion, and it is fixed on the lower section of reagent strip pallet, the top surface of the heating sheet fixed plate is arranged at intervals with multiple heating convex blocks upwards； The magnet assemblies, magnet assemblies power mechanism and magnet up and down optocoupler assembly be respectively positioned on tray substrate and reagent strip pallet it Between, and magnet assemblies are located at the lower section of heating sheet fixed plate and heating sheet；The magnet assemblies power mechanism is for rising or dropping Lower magnet assemblies, to complete the magnetic bead separation step to reagent strip；The magnet up and down optocoupler assembly be mounted on magnet assemblies with Between magnet assemblies power mechanism, for detecting magnet assemblies the location of in tray mold group ontology.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the magnet assemblies include that magnet is fixed Seat, multiple big magnet, two guide posts and two guide pillar springs；The bottom of two guide posts is fixed on tray substrate, the magnet fixing seat Being sleeved on two guide posts via corresponding linear bearing can move up and down, and two guide pillar springs are set on two guide posts, and are pushed up Between magnet fixing seat and heating sheet fixed plate, moved down for auxiliary magnet fixing seat；Between all big magnet are vertical Every being arranged in magnet fixing seat, and be in its corresponding to open slot rear end extended line on, the top of each big magnet Portion is crossed to form the conical surface by two inclined top faces along the length direction of open slot；Corresponding, between two heating convex blocks heating The magnet via hole that the corresponding big magnet of adaptation passes through is provided in piece fixed plate.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the magnet assemblies power mechanism includes Motor mounting plate, DC brushless motor, eccentric disc and bearing and its connecting shaft；The motor mounting plate is vertically mounted on tray bottom On plate, and the motor mounting plate is big facing towards magnet fixing seat；The DC brushless motor is secured transverse to motor mounting plate On, and the output shaft of the DC brushless motor passes through motor mounting plate towards magnet fixing seat；The eccentric disc is connected to direct current On the output shaft of brushless motor, the bearing and its connecting shaft are fixed in the disk of eccentric disc, and fixed position is deviateed directly Flow the output shaft of brushless motor；The side of magnet fixing seat towards the magnet assemblies power mechanism is horizontally arranged with adaptation bearing And its slotted hole that connecting shaft rolls in it, it is driven greatly under the driving of DC brushless motor, stirring magnet fixing seat Magnet rises or falls.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the puncture liquid relief mould group includes puncturing Liquid relief stepper motor, puncture liquid relief synchronous belt component, puncture liquid relief screw drive component, liquid relief move back tube assembly, the selection of withdraw of the needle head Component punctures liquid relief guide rail slide block component and big sliding panel；The liquid relief moves back tube assembly and withdraw of the needle head selection component is installed in The big positive middle and lower part of sliding panel；The back side of the big sliding panel is by screw and punctures the sliding block in liquid relief guide rail slide block component It is connected, two guide rails punctured in liquid relief guide rail slide block component are fixed on the front of rack by screw interval vertical；Institute It states and punctures the back side that liquid relief stepper motor is vertically mounted on rack via corresponding fixing piece, and the puncture liquid relief stepper motor Output shaft is arranged upward, and is connected via corresponding bearing with the synchronous pulley punctured in liquid relief synchronous belt component；Institute It states and punctures the back side that the screw rod in liquid relief screw drive component is also vertically mounted on rack via corresponding bearing and fixing piece, and The upper end of the screw rod is connected via corresponding bearing with another synchronous pulley punctured in liquid relief synchronous belt component；It is described to wear Nut in thorn liquid relief screw drive component is mounted on the back side of big sliding panel via corresponding fixing piece, and the nut is located at institute In the longitudinal central axis line for stating big sliding panel；The synchronous belt punctured in liquid relief synchronous belt component is sleeved on the two synchronous pulleys On, for driving screw rod rotation under the driving for puncturing liquid relief stepper motor, and the nut by matching with the screw rod drives Big sliding panel and its sliding block at the back side are slided up and down along guide rail, and thus the related positive liquid relief of big sliding panel that is mounted on moves back tube assembly It is integrally moved up and down with withdraw of the needle head selection component.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: it includes liquid relief that the liquid relief, which moves back tube assembly, Move back pipe motor fixing plate, liquid relief moves back pipe screw motor, liquid relief moves back pipe guide rail slide block component, small sliding panel, syringe push plate, liquid relief Move back pipe motor nut, multiple puncture needles, syringe fixed plate, more liquid relief syringes and optocoupler sensor；The liquid relief moves back pipe Motor fixing plate is laterally vertically fixed on the front of big sliding panel, and the liquid relief, which moves back pipe screw motor and is mounted on liquid relief, moves back pipe motor In fixed plate, and the liquid relief moves back the screw rod of pipe screw motor and passes through liquid relief and move back pipe motor fixing plate and be arranged vertically downward；The shifting Liquid move back two guide rails in pipe guide rail slide block component by screw perpendicular separation be fixed on liquid relief move back it is big below pipe motor fixing plate Sliding panel front, the back side of the small sliding panel move back the sliding block in pipe guide rail slide block component with liquid relief and are connected, the syringe Push plate is fixed on the top of small sliding panel, and the liquid relief is moved back pipe motor nut and is mounted in the syringe push plate, and moves back with liquid relief The screw rod of pipe screw motor matches；Multiple puncture needle perpendicular separations are fixed on the bottom surface of small sliding panel；The syringe is solid Fixed board is located at the lower section that liquid relief moves back pipe motor fixing plate, and moves back pipe motor fixing plate with liquid relief via two root posts and be connected；It is more Branch liquid relief syringe perpendicular separation is mounted on the top surface of syringe fixed plate, and the front of the syringe push plate is more along being provided with Card slot of a adapter more liquid relief syringe tail portions；The optocoupler sensor is connected to liquid relief via corresponding fixing piece and moves back Big sliding panel front below pipe motor fixing plate.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the withdraw of the needle head assembly includes multiple moves back Syringe needle column, multiple withdraw of the needle plates, withdraw of the needle head stepper motor, withdraw of the needle head synchronous belt component and withdraw of the needle head guide rail slide block component；Wherein, often Two withdraw of the needle headpins are installed via corresponding linear bearing in the syringe fixed plate at a liquid relief syringe rear, are moved back each The upper half of syringe needle column is set with a spring, and the lower end of the two withdraw of the needle headpins is all connected with one block of withdraw of the needle plate, the withdraw of the needle plate Front end is provided with the notch that an adaptation returns liquid relief injector head syringe needle；The withdraw of the needle head stepper motor is via corresponding fixed Part lateral inward is connected to the side of small sliding panel, and the output shaft of the withdraw of the needle head stepper motor is via corresponding bearing and the withdraw of the needle Head synchronous belt component in a synchronous pulley be connected, another synchronous pulley in the withdraw of the needle head synchronous belt component via Corresponding bearing and connecting shaft are connected to the other side of small sliding panel；Guide rail in the withdraw of the needle head guide rail slide block component passes through spiral shell Nail is secured transverse to the positive lower end of small sliding panel, and the sliding block in the withdraw of the needle head guide rail slide block component is via corresponding connector Be connected with the synchronous belt in withdraw of the needle head synchronous belt component, and the synchronous belt be sleeved on two in withdraw of the needle head synchronous belt component it is same It walks on belt wheel.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the darkroom door gear include dark-room door, Two dark-room door guide rail slide block components and dark-room door power mechanism；The back side of the dark-room door is respectively arranged with two darkrooms of adaptation insertion The groove and sliding block connecting hole of door guide rail slide block component, the dark-room door pass through these sliding block connecting holes by screw and are connected to darkroom On sliding block in door guide rail slide block component, the guide rail in the dark-room door guide rail slide block component is vertically fixed on rack by screw Front；The side of the dark-room door is connected via corresponding dark-room door connector with dark-room door power mechanism；The darkroom Door power mechanism is by dark-room door stepper motor, aforementioned dark-room door connector, dark-room door synchronous belt component, dark-room door power mechanism branch Holder assembly composition；Synchronous belt in the dark-room door synchronous belt component is vertically positioned at the side of dark-room door, aforementioned dark-room door connection Part is connected by screw between synchronous belt and dark-room door；In the upper end of the synchronous belt, the dark-room door stepper motor is via dark Dark-room door stepper motor support in the door power mechanism mounting assembly of room is fixed on the front of rack, the dark-room door synchronous belt group A synchronous pulley in part is connected on the output shaft of dark-room door stepper motor via corresponding bearing；Under the synchronous belt It holds, the synchronous belt support in the dark-room door power mechanism mounting assembly is fixed on the front of rack, the dark-room door synchronous belt Another synchronous pulley in component is connected on the synchronous belt support via corresponding bearing and connecting shaft；The synchronous belt set On the two synchronous pulleys.

The Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument, in which: the readings mould group is by readings X-axis power Mechanism, readings Y-axis power mechanism, lens assembly and bar code device composition；The readings X-axis power mechanism and readings Y-axis engine Structure is respectively used to be also used to Y direction moving lens component and readings X-axis power mechanism along the x axis in the horizontal plane In the horizontal plane along the x axis moving lens component while related Mobile barcode device；The lens assembly is used for being placed on The Reagent Tube of readings is needed to carry out effective light-emitting data acquisition in tray mold group on multiple reagent strips, the bar code device is for reading Bar code information on multiple reagent strips；The readings X-axis power mechanism includes readings X-axis stepper motor, readings X-axis synchronizing wheel Component, readings X-axis guide rail slide block assembly and readings X-axis fixed plate；The readings X-axis fixed plate is erected at machine via two root posts On the bottom plate of frame；The X-axis stepper motor is mounted on the vertical plate front of rack, and the X via corresponding fixing piece along the y axis The output shaft of shaft step motor passes through the vertical plate and via a synchronous belt in corresponding bearing wheel assembly synchronous with readings X-axis Wheel is connected, another synchronous pulley in the synchronous wheel assembly of the readings X-axis is via corresponding bearing, connecting shaft and fixing piece It is connected to the vertical plate back side of rack；Guide rail in the readings X-axis guide rail slide block assembly is fixed on this by screw along the x axis In readings X-axis fixed plate between two synchronous pulleys, the bottom of readings Y-axis fixed plate one end in the readings Y-axis power mechanism Face is connected with the sliding block in readings X-axis guide rail slide block assembly, and the top surface of the readings Y-axis fixed plate one end via connecting accordingly Synchronous belt in fitting wheel assembly synchronous with readings X-axis is connected；The readings Y-axis power mechanism includes readings Y-axis stepping electricity The synchronous wheel assembly of machine, readings Y-axis, readings Y-axis guide rail slide block assembly, aforementioned readings Y-axis fixed plate, readings Y-axis idler wheel and readings Y Axis support plate；Wherein, the readings Y-axis support plate is also erected on the bottom plate of rack via two root posts, the readings Y-axis rolling Wheel is mounted on the other end of readings Y-axis fixed plate；The y-axis stepper motor is mounted on along the x axis via corresponding fixing piece The idler wheel end of readings Y-axis fixed plate, and the output shaft of the y-axis stepper motor is via corresponding bearing and readings Y-axis synchronizing wheel group A synchronous pulley in part is connected, another synchronous pulley in the synchronous wheel assembly of the readings Y-axis is via corresponding axis It holds, connecting shaft and fixing piece are fixed on the other end of readings Y-axis fixed plate；Guide rail in the readings Y-axis guide rail slide block assembly It is fixed on along the y axis by screw in the readings Y-axis fixed plate between this two synchronous pulley, the lens assembly and readings Y Sliding block in axis rail slide block assembly is connected, and the synchronous belt in lens assembly wheel assembly synchronous with readings Y-axis is connected； The lens assembly includes photomultiplier tube, direct-connected guide-lighting projection seat and direct-connected guide-lighting dimple；The photomultiplier tube is mounted on directly Even in guide-lighting projection seat, the direct-connected guide-lighting projection seat is mounted on direct-connected guide-lighting dimple；The direct-connected guide-lighting projection seat and aforementioned readings Y Synchronous belt in the synchronous wheel assembly of axis is connected, the direct-connected guide-lighting dimple and the sliding block phase in readings Y-axis guide rail slide block assembly Connection；The bar code device is fixed in readings Y-axis fixed plate via corresponding fixing piece.

A kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument provided by the present invention, due to using interior mistress's Rational deployment, in conjunction with the design of the compact science to correlation function mould group, not only structure it is simpler it is compact, cost is less expensive, And high degree of automation, be particularly suitable for using instant reagent, can detect one or more parts sample simultaneously, sample dosage compared with Few, operation is also easy to be quick, and you can get it within 30 minutes as a result, greatly reducing patient's waiting time, and testing result is also more steady It is fixed；The medical institutions for being very suitable to China use, popularize.

Detailed description of the invention

Fig. 1 is that the perspective view of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument embodiment of the present invention (has taken away darkroom Shell)；

Fig. 2 is the perspective view of the tray mold group in Fig. 1 of the present invention；

Fig. 3 is the amplification stereogram (splitting from the 1st slot) of the tray mold group ontology in Fig. 2 of the present invention；

Fig. 4 is the amplification stereogram of the magnet assemblies and magnet engine structure in Fig. 3 of the present invention；

Fig. 5 is the amplification stereogram of agents useful for same item in Fig. 2 of the present invention；

Fig. 6 is the amplification stereogram of the puncture liquid relief mould group in Fig. 1 of the present invention；

Fig. 7 is the amplification stereogram that liquid relief in Fig. 6 of the present invention moves back tube assembly；

Fig. 8 is the amplification stereogram of the withdraw of the needle head selection component in Fig. 6 of the present invention；

Fig. 9 is the amplification stereogram of the darkroom door gear in Fig. 1 of the present invention；

Figure 10 is the amplification stereogram of the barcode scanning readings mould group in Fig. 1 of the present invention.

Specific embodiment

Below with reference to attached drawing, a specific embodiment of the invention and embodiment are described in detail, described tool Body embodiment only to explain the present invention, is not intended to limit a specific embodiment of the invention.

As shown in Figure 1, Fig. 1 is the perspective view of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument embodiment of the present invention (having taken away darkroom shell), the Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument include rack 1, tray mold group 2, puncture and move Liquid mould group 4, darkroom shell (not shown go out), darkroom door gear 5 and barcode scanning readings mould group 6；Wherein:

The rack 1 connects into inverted T-shape by horizontal bottom plate 11 and vertical supporting plate component 12, entire instrument is divided into interior Two parts in room and mistress；The supporting plate component 12 forms frame shape knot by instrument left plate, right side plate, vertical plate and vertical plate upper piece Structure；The bottom of the left plate and right side plate is separately fixed at the two sides of bottom plate 11, and the vertical plate is arranged in left plate and right side Between plate, and there are the evacuation notch that interior mistress's lower layer space is passed in and out convenient for tray mold group 2, the vertical plates upper piece for the lower section of vertical plate Positioned at the top of vertical plate, and be connected at the top of left plate and right side plate.

An electronic component box 7 is fixed on the supporting plate component 12, for installing power supply, circuit board, radiator fan It is located at the top area of interior room with relative electronic components, the electronic component boxes 7 such as switch keys, belongs to the inactive of instrument Region；The electronic component box 7 is by power supply folded plate, fan folded plate and anti-tampering board group at the two sides of the power supply folded plate are in Triangle, front end are arranged on the left plate and right side plate of supporting plate component 12, and the fan folded plate is located at power supply folded plate Rear end, the anti-tampering plate are located at the top of power supply folded plate.

The tray mold group 2 is located at the lower region of mistress, for placing and fixing multiple reagent strips to be detected 3, and Reagent strip 3 is sent into the lower region of interior room or is exited to the lower region of mistress；

The liquid relief mould group 4 that punctures is located at the top area of mistress, can realize its whole automatic lifting, automatically by process control It loads or is detached from suction pipette head, for piercing through the film and automatic sucking that are covered on Reagent Tube top surface on reagent strip 3 automatically Or the liquid inside its Reagent Tube is discharged；

The darkroom shell is located at the lower region of interior room, is used to form the darkroom space for blocking or obstructing ambient, because The barcode scanning readings mould group 6 has blocked strict requirements to light；For the inside convenient for showing instrument interior room lower region Structure, Fig. 1 have taken away the darkroom casing part；

The darkroom door gear 5 is arranged on the vertical plate of supporting plate component 12, retractable for cooperating darkroom shell to form side Darkroom space；

The barcode scanning readings mould group 6 is located at the lower region of interior room, and is in the inside of darkroom shell, can be real by process control Existing horizontal direction X-axis and Y-axis automatically move, for excite and read be incubated for after reagent strip 3 fluorescence information.

Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument of the invention, by using the rational deployment of interior mistress, knot Close the design to the compact science of correlation function mould group, not only structure it is simpler it is compact, cost is less expensive, but also improve instrument The degree of automation of device entirety is particularly suitable for using instant reagent, can detect one or more parts sample simultaneously, be very suitable to The medical institutions of the country of state use, popularize.

In the preferred embodiment of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument of the present invention, as shown in connection with fig. 2, Fig. 2 is the perspective view of the tray mold group in Fig. 1 of the present invention, specifically, the tray mold group 2 is by 21 He of tray mold group power mechanism Tray mold group ontology 22 forms, and the tray mold group ontology 22 is mounted on tray mold group power mechanism 21, the tray mold group Power mechanism 21 is used to that the tray mold group ontology 22 for being installed with multiple reagent strips 3 side by side to be sent into the lower region of interior room or be exited To the lower region of mistress, what Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument embodiment of the present invention and its attached drawing provided is It can at most be put into the tray mold group 2 of 5 reagent strips 3 simultaneously.

Specifically, the tray mold group power mechanism 21 includes 211, two pallet sliding rails 212 of pallet stepper motor, coding Device 214 and pallet synchronous belt component 215；The pallet synchronous belt component 215 is located therein the top of a pallet sliding rail 212, The tray mold group ontology 22 is erected on two pallet sliding rails 212, and the side of the tray mold group ontology 22 is via corresponding Connector be connected with the synchronous belt in pallet synchronous belt component 215, for the drive lower edge in pallet synchronous belt component 215 The length direction of pallet sliding rail 212 moves back and forth；It is synchronous that the pallet stepper motor 211 with encoder 214 is located at pallet Both ends with component 215, and 212 liang of pallet sliding rail of 215 lower section of pallet synchronous belt component are fixed on via respective fixing piece End, and via in pallet synchronous belt component 215 synchronous pulley and corresponding bearing it is synchronous in pallet synchronous belt component 215 V belt translation connection；The encoder 214 needs to reach for cooperating pallet stepper motor 211 to rest on tray mold group ontology 22 Position, when the encoder 214 detection pallet stepper motor 211 operation to tray mold group ontology 22 need reach position when, Circuit board in feedback signal electron component box 7 stops sending pulse signal to pallet stepper motor 211, thus in darkroom When door gear 5 is opened, the tray mold group ontology 22 equipped with reagent strip 3 is passed through to the evacuation of the vertical plate bottom in supporting plate component 12 Notch is sent into interior room lower region or exits to the accurate location of mistress's lower region.

In conjunction with shown in Fig. 3 and Fig. 4, Fig. 3 is the amplification stereogram of the tray mold group ontology in Fig. 2 of the present invention (from the 1st slot Split at place), Fig. 4 is the amplification stereogram of the magnet assemblies and magnet engine structure in Fig. 3 of the present invention；Specifically, the pallet Mould group ontology 22 includes tray substrate 221, needle tubing tray 222, reagent strip pallet 223, reagent strip tabletting 224, reagent strip clip 228, optocoupler above and below heating sheet 225, heating sheet fixed plate 2251, magnet assemblies 226, magnet assemblies power mechanism 227 and magnet Component 229；It is specific:

The tray substrate 221 is erected on two pallet sliding rails 212 of tray mold group power mechanism 21 described in Fig. 2, in the support The try to get to the heart of a matter side of plate 221 is fixed with connector for being connected with the synchronous belt in pallet synchronous belt component 215 (such as Fig. 2 institute Show)；

The needle tubing tray 222 and reagent strip pallet 223 are fixed on tray substrate via corresponding left and right supporting element in Fig. 2 221 top；Wherein, it is correspondingly arranged on the needle tubing tray 222 multiple rows of for placing the syringe needle hole of three syringe needles 2222 2221, every row's syringe needle hole 2221 be respectively positioned on its corresponding to 2231 rear end of open slot extended line on, the needle tubing tray 222 On be additionally provided with multiple sample wells 2223, for placing the test tube (out not shown) equipped with (nucleic acid) sample to be detected, each sample Sample wells 2223 be also respectively positioned on its corresponding to 2231 rear end of open slot extended line on, and the syringe needle hole set in a row 2221 Between corresponding open slot 2231 and sample well 2223；

And being arranged side by side on the reagent strip pallet 223 has multiple open slots 2231 for being adapted to and being put into reagent strip 3 described in Fig. 2, uses In the movement of limitation 3 left and right directions of reagent strip；The reagent strip tabletting 224 is distributed in the reagent strip pallet of 2231 two sides of open slot On 223 top surface, for pushing down the reagent strip 3 of push-in open slot 2231, to limit the movement of 3 up and down direction of reagent strip；It is described Reagent strip clip 228 is located on the bottom surface of the reagent strip pallet 223 of 2231 front end of open slot, for blocking 3 front end of reagent strip Test tube outer wall, moved in the front-back direction with limiting reagent strip 3.

The heating sheet 225 is mounted on the bottom surface of heating sheet fixed plate 2251, and the heating sheet fixed plate 2251, which is located at, to be opened The tail portion of mouth slot 2231, and it is fixed on the lower section of reagent strip pallet 223, the top surface of the heating sheet fixed plate 2251 is spaced upwards Multiple heating convex blocks 2252 are provided with, the heat that the heating sheet 225 issues is transmitted to heating by heating sheet fixed plate 2251 Reagent Tube on convex block 2252, for heating and being located between two heating convex blocks 2252 on constant temperature reagent strip 3；The heating sheet 225 are initially powered up heating when instrument is switched on, and multiple 2252 constant temperature of heating convex block in heating sheet fixed plate 2251 are existed 37 DEG C, until just being powered off when instrument shutdown.

Optocoupler assembly 229 is respectively positioned on tray bottom up and down for the magnet assemblies 226, magnet assemblies power mechanism 227 and magnet Between plate 221 and reagent strip pallet 223, and magnet assemblies 226 are located at the lower section of heating sheet fixed plate 2251 and heating sheet 225； The magnet assemblies power mechanism 227 is for rising or falling magnet assemblies 226, to complete the Beads enrichment step to reagent strip 3 Suddenly；Optocoupler assembly 229 is mounted between magnet assemblies 226 and magnet assemblies power mechanism 227 magnet up and down, for detecting Magnet assemblies 226 are in upper or the next the location of in tray mold group ontology 22.

Specifically, the magnet assemblies 226 include magnet fixing seat 2261, multiple big magnet 2262, two guide posts, 2263 and Two guide pillar springs 2264；The bottom of two guide posts 2263 is fixed on tray substrate 221, and the magnet fixing seat 2261 is via corresponding Linear bearing be sleeved on two guide posts 2263 and can move up and down, two guide pillar springs 2264 are set on two guide posts 2263, And withstand between magnet fixing seat 2261 and heating sheet fixed plate 2251, it is moved down for auxiliary magnet fixing seat 2261；Institute Big magnet 2262 is stated in cuboid and there is magnetism, the equal perpendicular separation of all big magnet 2262 is arranged in magnet fixing seat 2261 On, and be in its corresponding to 2231 rear end of open slot extended line on, the top of each big magnet 2262 is along open slot 2231 length direction is crossed to form the conical surface by two inclined top faces；Corresponding, between two heating convex blocks 2252 heating sheet The magnet via hole 2253 that the corresponding big magnet 2262 of adaptation passes through is provided in fixed plate 2251, in order to 2261 band of magnet fixing seat Big magnet 2262 is moved to rise or fall.

Specifically, the magnet assemblies power mechanism 227 include motor mounting plate 2271, DC brushless motor 2272, partially Cartridge 2273 and bearing and its connecting shaft 2274；The motor mounting plate 2271 is vertically mounted on tray substrate 221, and the electricity Machine mounting plate 2271 it is big facing towards magnet fixing seat 2261；The DC brushless motor 2272 is secured transverse to motor mounting plate On 2271, and the output shaft of the DC brushless motor 2272 passes through motor mounting plate 2271 towards magnet fixing seat 2261；It is described Eccentric disc 2273 is connected on the output shaft of DC brushless motor 2272, and the bearing and its connecting shaft 2274 are fixed on eccentric disc In 2273 disk, and the output shaft of DC brushless motor 2272 is deviateed in fixed position；2261 direction of magnet fixing seat The slotted hole that the side of magnet assemblies power mechanism 227 is horizontally arranged with adaptation bearing and its connecting shaft 2274 rolls in it 2275, under the driving of DC brushless motor 2272, stir magnet fixing seat 2261 drive big magnet 2262 rise or under Drop；To overcome eccentric wheel drive mechanism itself, there are the defects of locked-up point, and aforementioned two guide pillar spring 2264 is in brush DC electricity In the rotation process of machine 2272, moreover it is possible to play the role of that eccentric disc 2273 is prevented to be stuck.

As shown in connection with fig. 5, Fig. 5 is the amplification stereogram of agents useful for same item in Fig. 2 of the present invention, specifically, the reagent strip 3 from It is left-to-right successively by the first inclined-plane Reagent Tube 311, the second inclined-plane Reagent Tube 312, the detachable reagent pipe sleeve 321 of the first cylinder, The detachable reagent pipe sleeve 322 of two cylinders, the first oval Reagent Tube of the 331, first oval Reagent Tube 341, second of cylinder Reagent Tube 342, the second cylinder Reagent Tube 332, third cylinder Reagent Tube 333, the 4th cylinder Reagent Tube 334, the 5th cylinder Reagent Tube 335, 6th cylinder Reagent Tube 336 and the 7th cylinder Reagent Tube 337 rearrange；Wherein, first inclined-plane Reagent Tube 311 and second Inclined-plane Reagent Tube 312 is located at the tail portion of reagent strip 3, is used for Beads enrichment, the detachable reagent pipe sleeve 321 of first cylinder and the The detachable reagent pipe sleeve 322 of two cylinders is respectively used to be packed into the detachable Reagent Tube of the first cylinder and the detachable Reagent Tube of the second cylinder (Fig. 5 is not shown)；When the reagent strip 3 is pushed into, the reagent strip clip 228 of aforementioned tray mold group ontology 22, which can block, to be located at The outer wall of the 7th cylinder Reagent Tube 337 on 3 head of reagent strip.

As shown in connection with fig. 6, Fig. 6 is the amplification stereogram of the puncture liquid relief mould group in Fig. 1 of the present invention, specifically, described wear Piercing liquid relief mould group 4 includes puncturing liquid relief stepper motor 41, puncturing liquid relief synchronous belt component 42, puncture liquid relief screw drive component 43, liquid relief moves back tube assembly 44, withdraw of the needle head selection component 45, punctures liquid relief guide rail slide block component (not shown go out) and big sliding panel 46；It is specific:

The liquid relief moves back tube assembly 44 and withdraw of the needle head selection component 45 is installed in the positive middle and lower part of big sliding panel 46；It is described big The back side of sliding panel 46 is connected by screw with the sliding block punctured in liquid relief guide rail slide block component, and the puncture liquid relief guide rail is sliding Two guide rails in block assembly are fixed on the front of the vertical plate in Fig. 1 supporting plate component 12 by screw interval vertical；The puncture moves Liquid stepper motor 41 is vertically mounted on the vertical plate back side in Fig. 1 supporting plate component 12 via corresponding fixing piece, and the puncture moves The output shaft of liquid stepper motor 41 is arranged upward, and same via one in corresponding bearing and puncture liquid relief synchronous belt component 42 Step belt wheel is connected；

The screw rod punctured in liquid relief screw drive component 43 is also vertically mounted on Fig. 1 branch via corresponding bearing and fixing piece The vertical plate back side in frame plate component 12, and the upper end of the screw rod via corresponding bearing and punctures in liquid relief synchronous belt component 42 Another synchronous pulley is connected；The nut punctured in liquid relief screw drive component 43 is mounted on via corresponding fixing piece The back side of big sliding panel 46, and the nut is located in the longitudinal central axis line of the big sliding panel 46；The puncture liquid relief synchronous belt Synchronous belt in component 42 is sleeved on the two synchronous pulleys, for driving spiral shell under the driving for puncturing liquid relief stepper motor 41 Bar rotation, and the nut by matching with the screw rod drives the sliding block at big sliding panel 46 and its back side to slide up and down along guide rail, Thus related to be mounted on that the positive liquid relief of big sliding panel 46 moves back tube assembly 44 and withdraw of the needle head selection component 45 is whole moves up and down, from And it needs to complete to puncture according to detection, the movement that liquid relief and/or the selective withdraw of the needle are first-class.

Referring to figs. 7 and 8, Fig. 7 is the amplification stereogram that liquid relief in Fig. 6 of the present invention moves back tube assembly, and Fig. 8 is this hair The amplification stereogram of withdraw of the needle head selection component in bright Fig. 6；Specifically, it includes that liquid relief moves back pipe motor that the liquid relief, which moves back tube assembly 44, Fixed plate 442, liquid relief move back pipe screw motor 441, liquid relief moves back pipe guide rail slide block component 444, small sliding panel 451, syringe push plate 452, liquid relief moves back pipe motor nut 458, multiple puncture needles 456, syringe fixed plate 443, more liquid relief syringes 445 and optocoupler Sensor 457；It is specific:

The liquid relief moves back the front that pipe motor fixing plate 442 is laterally vertically fixed on big sliding panel 46, and the liquid relief moves back pipe screw rod Motor 441 is mounted on liquid relief and moves back on pipe motor fixing plate 442, and the liquid relief move back pipe screw motor 44 screw rod pass through liquid relief move back pipe Motor fixing plate 442 is arranged vertically downward；The liquid relief move back two guide rails in pipe guide rail slide block component 444 by screw it is vertical between Every being fixed on, the big sliding panel 46 that liquid relief is moved back below pipe motor fixing plate 442 is positive, the back side of the small sliding panel 451 and liquid relief The sliding block moved back in pipe guide rail slide block component 444 is connected, and the syringe push plate 452 is fixed on the top of small sliding panel 451, institute It states liquid relief and moves back pipe motor nut 458 and be mounted in the syringe push plate 452, and move back the screw rod phase of pipe screw motor 441 with liquid relief Cooperation, for being driven by moving back pipe motor nut 458 with the liquid relief that screw rod cooperates under the driving that liquid relief moves back pipe screw motor 44 The sliding block of syringe push plate 452, small sliding panel 451 and its back side is slided up and down along guide rail；Multiple 456 perpendicular separations of puncture needle are solid It is scheduled on the bottom surface of small sliding panel 451, the movement for needing to complete to puncture according to detection；

The syringe fixed plate 443 is located at liquid relief and moves back the lower section of pipe motor fixing plate 442, and moves back via two root posts and liquid relief Pipe motor fixing plate 44 is connected；More 445 perpendicular separations of liquid relief syringe are mounted on the top surface of syringe fixed plate 443, The front of the syringe push plate 452 is used for root along multiple adapters are provided in the card slot of more 445 tail portions of liquid relief syringe Need to complete the movement of liquid relief according to detection；

The optocoupler sensor 457 is connected to the big sliding that liquid relief moves back 442 lower section of pipe motor fixing plate via corresponding fixing piece 46 front of plate is in upper or the next for incuding the location of syringe push plate 452.

Specifically, the withdraw of the needle head assembly 45 includes multiple withdraw of the needle headpins 446, multiple withdraw of the needle plates 447, withdraw of the needle head stepping electricity Machine 453, withdraw of the needle head synchronous belt component 454 and withdraw of the needle head guide rail slide block component 455；Wherein, each 445 rear of liquid relief syringe Two withdraw of the needle headpins 446 are installed via corresponding linear bearing in syringe fixed plate 443, in each withdraw of the needle headpin 446 The upper half is set with a spring, and the withdraw of the needle headpin 446 for keeping it internal restores in the absence of external forces to initial bit It sets, and the lower end of the two withdraw of the needle headpins 446 is all connected with one block of withdraw of the needle plate 447, the front end of the withdraw of the needle plate 447 is provided with an adaptation Return the notch of 445 head syringe needle of liquid relief syringe；

The withdraw of the needle head stepper motor 453 is connected to the side of small sliding panel 451 via corresponding fixing piece lateral inward, and should The output shaft of withdraw of the needle head stepper motor 453 is via a synchronous pulley in corresponding bearing and withdraw of the needle head synchronous belt component 454 It is connected, another synchronous pulley in the withdraw of the needle head synchronous belt component 454 is connected to via corresponding bearing and connecting shaft The other side of small sliding panel 451；Guide rail in the withdraw of the needle head guide rail slide block component 455 is secured transverse to small sliding by screw The positive lower end of plate 451, the sliding block in the withdraw of the needle head guide rail slide block component 455 are synchronous with withdraw of the needle head via corresponding connector It is connected with the synchronous belt in component 454, and the synchronous belt is sleeved on two synchronous pulleys in withdraw of the needle head synchronous belt component 454 On, for being moved to needs along guide rail by synchronous belt band movable slider and returning under the driving of the withdraw of the needle head stepper motor 453 Subsequent two withdraws of the needle headpin, 446 top of liquid relief syringe 445 of syringe needle, and the driving of pipe screw motor 441 is moved back in the liquid relief Under, drive small sliding panel 451 and sliding block to move down by nut, to press corresponding two withdraws of the needle headpin 446 and overcome two springs Elastic force, linkage two withdraw of the needle headpins, 446 lower end withdraw of the needle plate 447 move down, completion return the 445 head syringe needle of liquid relief syringe Movement, and when small sliding panel 451 and sliding block move up, using the elastic force for two springs being sleeved on two withdraws of the needle headpin 446, Make two withdraws of the needle headpin 446 and connect the withdraw of the needle plate 447 of its lower end to move up return.

As shown in connection with fig. 9, Fig. 9 is the amplification stereogram of the darkroom door gear in Fig. 1 of the present invention, specifically, the darkroom Door gear 5 includes dark-room door 51, two dark-room door guide rail slide block components (not shown go out) and dark-room door power mechanism 52；The darkroom The back side of door 51 is respectively arranged with the groove 511 and sliding block connecting hole 512 of adaptation two dark-room door guide rail slide block components of insertion, this is dark Room door 51 passes through these sliding block connecting holes 512 by screw and is connected on the sliding block in dark-room door guide rail slide block component, described dark Guide rail in the door guide rail slide block component of room is vertically fixed on the vertical plate front of Fig. 1 supporting plate component 12 by screw；The darkroom The side of door 51 is connected via corresponding dark-room door connector 522 with dark-room door power mechanism 52, in dark-room door power It under the driving of mechanism 52, is risen or fallen by sliding block along guide rail, to cooperate darkroom shell to open or close under instrument interior room The space of layer region is used to avoid the disengaging of tray mold group 2 including opening or blocking the vertical plate lower part in the supporting plate component 12 The evacuation notch of interior mistress's lower layer space, and then play the role of opening or closing darkroom space；Closed state can play closing The effect in darkroom space, opening state can meet the tray mold group ontology 22 equipped with reagent strip 3 in the interior room lower region of instrument It is passed in and out between mistress's lower region.

Specifically, the dark-room door power mechanism 52 is by dark-room door stepper motor 521, aforementioned dark-room door connector 522, dark Room door synchronous belt component 523, dark-room door power mechanism mounting assembly 524 form；It is same in the dark-room door synchronous belt component 523 Step band is vertically positioned at the side of dark-room door 51, and aforementioned dark-room door connector 522 is connected by screw in synchronous belt and dark-room door 51 Between；In the upper end of the synchronous belt, the dark-room door stepper motor 521 is via in dark-room door power mechanism mounting assembly 524 The vertical plate that dark-room door stepper motor support is fixed on Fig. 1 supporting plate component 12 is positive, in the dark-room door synchronous belt component 523 One synchronous pulley is connected on the output shaft of dark-room door stepper motor 521 via corresponding bearing；In the lower end of the synchronous belt, Synchronous belt support in the dark-room door power mechanism mounting assembly 524 is fixed on the vertical plate front of Fig. 1 supporting plate component 12, institute Another synchronous pulley stated in dark-room door synchronous belt component 523 is connected to the synchronous belt branch via corresponding bearing and connecting shaft On seat；The synchronous belt is sleeved on the two synchronous pulleys, under the driving of dark-room door stepper motor 521, by dark Room door connector 522 drives dark-room door 51 to move up and down.

As shown in connection with fig. 10, Figure 10 is the amplification stereogram of the barcode scanning readings mould group in Fig. 1 of the present invention, specifically, described Readings mould group 6 is made of readings X-axis power mechanism 61, readings Y-axis power mechanism 62, lens assembly 63 and bar code device 64；It is described Readings X-axis power mechanism 61 and readings Y-axis power mechanism 62 are respectively used to mobile with Y direction along the x axis in the horizontal plane While lens assembly 63 and readings X-axis power mechanism 61 are also used in the horizontal plane moving lens component 63 along the x axis Related Mobile barcode device 64；The lens assembly 63 can be realized multiple in tray mold group 2 to being placed in Fig. 1 by process control The Reagent Tube of readings is needed to carry out effective light-emitting data acquisition on reagent strip 3, the bar code device 64 is for reading multiple reagent strips 3 On bar code information.

Specifically, the readings X-axis power mechanism 61 includes readings X-axis stepper motor 611, the synchronous wheel assembly of readings X-axis 612, readings X-axis guide rail slide block assembly 613 and readings X-axis fixed plate 614；Wherein, the readings X-axis fixed plate 614 is via two Root post is erected on the bottom plate 11 of rack 1 in Fig. 1；The X-axis stepper motor 611 via corresponding fixing piece along the y axis Be mounted in Fig. 1 supporting plate component 12 vertical plate front, and the output shaft of the X-axis stepper motor 611 pass through the vertical plate and via A synchronous pulley in corresponding bearing wheel assembly 612 synchronous with readings X-axis is connected, the synchronous wheel assembly of the readings X-axis Another synchronous pulley in 612 is connected to vertical in Fig. 1 supporting plate component 12 via corresponding bearing, connecting shaft and fixing piece Back；Guide rail in the readings X-axis guide rail slide block assembly 613 is fixed on this two synchronous pulley by screw along the x axis Between readings X-axis fixed plate 614 on, the bottom surface of 624 one end of readings Y-axis fixed plate in the readings Y-axis power mechanism 62 It is connected with the sliding block in readings X-axis guide rail slide block assembly 613, and the top surface of 624 one end of readings Y-axis fixed plate is via corresponding Connector wheel assembly 612 synchronous with readings X-axis in synchronous belt be connected, under the driving of X-axis stepper motor 611, Readings Y-axis power mechanism 62 and lens assembly 63 and bar code thereon are driven by the synchronous belt of the synchronous wheel assembly 612 of readings X-axis Device 64 moves back and forth along the x axis.

Specifically, the readings Y-axis power mechanism 62 includes readings y-axis stepper motor 621, the synchronous wheel assembly of readings Y-axis 622, readings Y-axis guide rail slide block assembly 623, aforementioned readings Y-axis fixed plate 624, readings Y-axis idler wheel 625 and readings Y-axis support plate 615；Wherein, the readings Y-axis support plate 615 is also erected on the bottom plate 11 of rack 1 in Fig. 1 via two root posts, the reading Value Y-axis idler wheel 625 is mounted on the other end of readings Y-axis fixed plate 624, for rolling around in readings Y-axis support plate 615； The y-axis stepper motor 621 is mounted on the idler wheel end of readings Y-axis fixed plate 624 via corresponding fixing piece along the x axis, and The output shaft of the y-axis stepper motor 621 is via a synchronous pulley in corresponding bearing wheel assembly 622 synchronous with readings Y-axis It is connected, another synchronous pulley in the synchronous wheel assembly 622 of the readings Y-axis is via corresponding bearing, connecting shaft and fixation Part is fixed on the other end of readings Y-axis fixed plate 624；Guide rail in the readings Y-axis guide rail slide block assembly 623 passes through screw edge Y direction is fixed in the readings Y-axis fixed plate 624 between this two synchronous pulley, the lens assembly 63 and readings Y-axis guide rail Sliding block in slide block assembly 623 is connected, and the synchronous belt in the lens assembly 63 wheel assembly 622 synchronous with readings Y-axis is connected It connects, for driving camera lens by the synchronous belt of the synchronous wheel assembly 622 of readings Y-axis under the driving of readings y-axis stepper motor 621 Component 63 moves back and forth along the y axis.

Specifically, the lens assembly 63 includes that photomultiplier tube (PMT) 631, direct-connected guide-lighting projection seat and direct-connected leaded light are recessed Seat 632；The photomultiplier tube (PMT) 631 is mounted in direct-connected guide-lighting projection seat, and the direct-connected guide-lighting projection seat is mounted on direct-connected lead On light dimple 632；Synchronous belt in direct-connected guide-lighting projection seat wheel assembly 622 synchronous with aforementioned readings Y-axis is connected, it is described directly Even guide-lighting dimple 632 is connected with the sliding block in readings Y-axis guide rail slide block assembly 623.

The bar code device 64 is fixed on the readings Y-axis fixed plate 624 of readings Y-axis power mechanism 62 via corresponding fixing piece On.

In the following, by taking conventional blood nucleic acid sample detection as an example, Full-automatic magnetic beads time-resolved fluoroimmunoassay of the present invention point The working principle of analyzer embodiment and detection process are as follows:

Step S800,1 ~ 5 is respectively put into multiple sample wells 2223 on Fig. 3 needle tubing tray 222 equipped with blood to be detected 1 ~ 5 reagent strip 3 is respectively put into corresponding open slot 2231 and push-tight forward, so that 1 ~ 5 by the test tube of (nucleic acid) sample Reagent strip clip 228 blocks test tube (i.e. the 7th cylinder Reagent Tube 337 of Fig. 5) outer wall of respective 3 front end of reagent strip respectively.

Step S810, start corresponding detection switch on instrument, dark-room door 51 is driven by Fig. 9 dark-room door stepper motor 521 It moves up；Tray mold group ontology 22 is driven to be moved to instrument along pallet sliding rail 212 with reagent strip 3 by Fig. 2 pallet stepper motor 211 Interior room lower layer space, by Figure 10 readings X-axis stepper motor 611 drive bar code device 64 move along the x axis, be successively read 1 ~ 5 Bar code on a reagent strip 3 is confirmed；Drive tray mold group ontology 22 with reagent by Fig. 2 pallet stepper motor 211 again Item 3 is moved to the lower section for puncturing liquid relief mould group 4, and cooperation Fig. 6 punctures liquid relief stepper motor 41 and big sliding panel 46 is driven to move down, related 1 ~ 5 puncture needle 456 of withdraw of the needle head selection 45 lower part of component, which successively punctures, is sealed in the thin of all Reagent Tube top surfaces on reagent strip 3 Film.

Step S820, instrument, will by being mounted on 1 ~ 5 liquid relief syringe 445 of 443 top surface of Fig. 7 syringe fixed plate It is loaded into blood (nucleic acid) sample in corresponding sample well 2223 and is opened positioned at Fig. 3 reagent strip pallet 223 is corresponding respectively In the first inclined-plane Reagent Tube 311 and the second inclined-plane Reagent Tube 312 on reagent strip 3 in mouthful slot 2231 and with magnetic bead therein into Row mixing (or stirring)；Specifically moving back pipe screw motor 441 by Fig. 7 liquid relief drives Fig. 8 syringe push plate 452 to move up and down, and drives 1 ~ 5 liquid relief syringes 445, which are completed to inhale, puts movement, and to realize liquid relief and mixing (or stirring), mixing (or stirring) therein passes through Realization is put in the multiple suction of liquid relief syringe 445.

It should be noted that due to can only be used there are three syringe needle 2222 in the syringe needle hole 2221 of row same in Fig. 3, for this purpose, It is relevant to each 3 first inclined-plane Reagent Tube 311 of reagent strip to use the same syringe needle 2222, all and the first inclined-plane Reagent Tube 311 relevant liquid reliefs and suction are put, and the same syringe needle 2222 is all made of；Equally, with each 3 second inclined-plane Reagent Tube 312 of reagent strip It is relevant to use another syringe needle 2222；Remaining third syringe needle 2222 is put dedicated for the liquid relief of enhancement solution and suction.

In step S820, every liquid relief syringe 445 is by replacing respective syringe needle 2222, by blood (core to be detected Acid) sample is loaded onto respectively in 3 first inclined-plane Reagent Tube 311 of corresponding reagent item and the second inclined-plane Reagent Tube 312, and is passed through multiple Suction is put to be mixed with magnetic bead therein.

When syringe needle 2222 is installed, specifically drives tray mold group ontology 22 to be moved to puncture by Fig. 2 pallet stepper motor 211 and move The syringe needle 2222 specified in Fig. 3 is located in immediately below the corresponding liquid relief syringe 445 of Fig. 7, is punctured by Fig. 6 by 4 lower section of liquid mould group Liquid relief stepper motor 41 drives big sliding panel 46 to move down, and the head of related 1 ~ 5 liquid relief syringe 445 moves down, and then loads onto phase The syringe needle 2222 answered.

Back off syringe needle 2222 when, by Fig. 8 withdraw of the needle head stepper motor 453 drive withdraw of the needle head guide rail slide block component 455 in cunning Block prolongs the movement of its guide rail, as shown in connection with fig. 7, is moved to two withdraw of the needle headpins, 446 top at corresponding 445 rear of liquid relief syringe, And pipe screw motor 441 is moved back by liquid relief, withdraw of the needle head guide rail slide block component 455 is driven to move back tube assembly 44 with respect to liquid relief and move down, so that moving back Sliding block in syringe needle guide rail slide block component 455 presses two withdraw of the needle headpins 446 being disposed below, and thus drives withdraw of the needle plate 447 It moves down, and then returns the syringe needle 2222 mounted in corresponding 445 head of liquid relief syringe, the syringe needle 2222 backed off also is placed on original needle In head bore 2221, to install other syringe needle 2222, thus achieve the purpose that replace syringe needle 2222.

Step S830, every liquid relief syringe 445 is by replacing respective syringe needle 2222, respectively by corresponding reagent article 3 the It is oblique that neutralizer correspondence in four cylinder Reagent Tubes 334 and the 5th cylinder Reagent Tube 335 is loaded onto the first inclined-plane Reagent Tube 311 and two In face Reagent Tube 312, and capable mixing is put by repeatedly inhaling.

Step S840, after one section of specified incubation time, consolidated by 2272 drive magnet of Fig. 4 DC brushless motor Reservation 2261 moves up, and the first inclined-plane Reagent Tube 311 and second that related 1 ~ 5 piece big magnet 2262 rises to corresponding reagent strip 3 is oblique Between face Reagent Tube 312, with the magnetic bead in the first inclined-plane of magnetic Reagent Tube 311 and the second inclined-plane Reagent Tube 312 and it is moved to it On inclined-plane.

Step S850, every liquid relief syringe 445 draws corresponding reagent item 3 by replacing respective syringe needle 2222 respectively Reaction liquid in first inclined-plane Reagent Tube 311 and the second inclined-plane Reagent Tube 312, and it is discharged to the magnetic bead on respective inclined-plane On；This step repeated several times.

Step S860, every liquid relief syringe 445 draws corresponding reagent item 3 by replacing respective syringe needle 2222 respectively Cleaning solution in first oval Reagent Tube 341 and the second oval Reagent Tube 342, cleans the magnetic bead on respective inclined-plane；This Step also needs repeated several times, is repeated and washes away；And when last time is cleaned, driven by Fig. 4 DC brushless motor 2272 Magnet fixing seat 2261 moves down, and related 1 ~ 5 piece big magnet 2262 declines, to liquid relief syringe 445 with cleaning solution by respective inclined-plane On magnetic bead wash away to the bottom of Reagent Tube, and siphon away cleaning after waste liquid.

It should be noted that the waste liquid after cleaning can be placed on corresponding reagent item in otiose Reagent Tube, such as do not have 7th cylinder Reagent Tube 337, for another example spare the second cylinder Reagent Tube 332 and third cylinder Reagent Tube 333, have been used for another example That crosses is used to fill the 4th cylinder Reagent Tube 334 and the 5th cylinder Reagent Tube 335 of neutralizer, useless after can be adopted to dress cleaning Liquid.

Step S870, every liquid relief syringe 445 is by replacing respective syringe needle 2222, by the circle of corresponding reagent item 3 first Marker dilution in column Reagent Tube 331 is added separately to the detachable Reagent Tube 321 of the first cylinder and of respective reagent strip 3 In the marker of the detachable Reagent Tube 322 of two cylinders, and capable mixing is put by repeatedly inhaling；Wherein, it is removable to put the first cylinder for suction When unloading the marker being diluted in Reagent Tube 321, using syringe needle 2222 relevant to the first inclined-plane Reagent Tube 311, suction puts second When the marker being diluted in the detachable Reagent Tube 322 of cylinder, using syringe needle 2222 relevant to the second inclined-plane Reagent Tube 312.

Step S880, it is removable to draw the first cylinder by replacing respective syringe needle 2222 respectively for every liquid relief syringe 445 It unloads the marker after being diluted in Reagent Tube 321 and the detachable Reagent Tube 322 of the second cylinder and is added to corresponding first inclined-plane reagent In pipe 311 and two inclined-plane Reagent Tubes 312, and capable mixing is put by repeatedly inhaling.

Step S890, it is moved up by 2272 drive magnet fixing seat 2261 of Fig. 4 DC brushless motor, related 1 ~ 5 block of big magnet 2262 rise between the first inclined-plane Reagent Tube 311 of corresponding reagent strip 3 and the second inclined-plane Reagent Tube 312, oblique with magnetic first Face Reagent Tube 311 and magnetic bead in the second inclined-plane Reagent Tube 312 are simultaneously moved on its inclined-plane.(identical as step S840)

Step S900, every liquid relief syringe 445 draws corresponding reagent item 3 first by replacing respective syringe needle 2222 respectively Reaction liquid in inclined-plane Reagent Tube 311 and the second inclined-plane Reagent Tube 312, and be discharged on the magnetic bead on respective inclined-plane；This Step repeated several times.(identical as step S850)

Step S910, the magnetic bead in the first inclined-plane Reagent Tube 311 and the second inclined-plane Reagent Tube 312 is once cleaned again, weight Multiple step S860.

Step S920, every replacement of liquid relief syringe 445 is drawn dedicated for the third branch syringe needle 2222 of absorption enhancement solution Enhancement solution in 6th cylinder Reagent Tube 336 is loaded onto respectively in the first inclined-plane Reagent Tube 311 and the second inclined-plane Reagent Tube 312, And capable mixing is put by repeatedly inhaling.

Step S930, drive tray mold group ontology 22 with the reagent strip 3 for being incubated for completion by Fig. 2 pallet stepper motor 211 Enter the interior room lower layer space of instrument along pallet sliding rail 212；It drives dark-room door 51 to move down by Fig. 9 dark-room door stepper motor 521, seals Close the space in the shell of darkroom.

Step S940, lens assembly 63 is driven to stop by Figure 10 readings X-axis stepper motor 611 and readings y-axis stepper motor 621 3 top of reagent strip for needing readings is stayed in, and excites and read the first inclined-plane Reagent Tube 311 and the second inclined-plane of the reagent strip 3 The fluorescence information of Reagent Tube 312, to complete the acquisition of light-emitting data.

Step S940, dark-room door 51 is driven to move up by Fig. 9 dark-room door stepper motor 521；By Fig. 2 pallet stepper motor 211 Driving tray mold group ontology 22 is moved to mistress's lower layer space of instrument with reagent strip 3 along pallet sliding rail 212；It is dark by Fig. 9 again Room door stepper motor 521 drives dark-room door 51 to move down；Reagent strip 3 on tray mold group ontology 22 to be taken away.

As it can be seen that Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument of the invention is when being detected, it is only necessary to will be to The reagent strip of detection is placed in reagent strip pallet and is pushed into clamping, entire instrument can realize to reagent strip to be detected into Row puncture, reagent adding, liquid inhale a series of automation steps for putting stirring, cleaning, incubation, Beads enrichment, the luminous detection in darkroom, Therefore the degree of automation of entire magnetic bead time-resolved fluorescence immunoassay instrument is higher；One or more parts sample can be detected simultaneously, Sample dosage is less, easy to operate quick, and you can get it within 30 minutes as a result, greatly reducing the waiting time of patient, and detection is tied Fruit is also more stable.

It should be understood that the foregoing is merely illustrative of the preferred embodiments of the present invention, it is not sufficient to limit of the invention Technical solution within the spirit and principles in the present invention, can add according to the above description for those of ordinary skills With increase and decrease, replacement, transformation or improvement, and all these increases and decreases, replacement, transformation or improved technical solution, it all should belong to this The protection scope of invention appended claims.

Claims

1. a kind of Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument characterized by comprising rack, punctures tray mold group Liquid relief mould group, darkroom shell, darkroom door gear and barcode scanning readings mould group；Wherein: entire instrument is divided into interior room and mistress by rack Two parts；The top area of interior room is fixed with electronic component box, and darkroom shell is located at the lower region of interior room, dark-room door dress It sets and is arranged on the rack, for cooperating darkroom shell to form the openable and closable darkroom space in side；Tray mold group is located under mistress Layer region is sent into the lower region of interior room or exits for placing and fixing multiple reagent strips to be detected, and by reagent strip To the lower region of mistress；The top area that liquid relief mould group is located at mistress is punctured, is covered on examination on reagent strip for piercing through automatically Its internal liquid is discharged in the film and automatic sucking in agent tube top face；Barcode scanning readings mould group is located at the lower region of interior room, And be in darkroom shell inside, for excite and read be incubated for after reagent strip fluorescence information.

2. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 1, it is characterised in that: the pallet Mould group is made of tray mold group power mechanism and tray mold group ontology, and the tray mold group ontology is mounted on tray mold group engine On structure, the tray mold group power mechanism is for being sent into the tray mold group ontology for being installed with multiple reagent strips side by side to interior room Layer region is exited to the lower region of mistress；The tray mold group power mechanism includes pallet stepper motor, two pallets cunnings Rail, encoder and pallet synchronous belt component；The pallet synchronous belt component is located therein the top of a pallet sliding rail, the support Disk mould group ontology is erected on two pallet sliding rails, and the side of the tray mold group ontology is via corresponding connector and pallet Synchronous belt in synchronous belt component is connected；The pallet stepper motor and encoder are located at the two of pallet synchronous belt component End, and the pallet sliding rail both ends below pallet synchronous belt component are fixed on via respective fixing piece, and via pallet synchronous belt Synchronous pulley and corresponding bearing in component are connect with the toothed belt transmission in pallet synchronous belt component；The encoder is used for Tray mold group ontology is rested on the position for needing to reach by cooperation pallet stepper motor.

3. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 2, it is characterised in that: the pallet Mould group ontology includes tray substrate, needle tubing tray, reagent strip pallet, reagent strip tabletting, reagent strip clip, heating sheet, heating sheet Optocoupler assembly above and below fixed plate, magnet assemblies and magnet assemblies power mechanism and magnet；The tray substrate is erected at two supports On disk sliding rail, the connection for being connected with the synchronous belt in pallet synchronous belt component is fixed in the side of the tray substrate Part；The needle tubing tray and reagent strip pallet are fixed on the top of tray substrate via corresponding left and right supporting element；The needle Be correspondingly arranged on conduit saddle block it is multiple rows of for placing the syringe needle holes of three syringe needles, every row's syringe needle hole be respectively positioned on its corresponding to open On the extended line of mouth slot rear end, multiple sample wells are additionally provided on the needle tubing tray, sample to be tested is housed for placing Test tube, each sample well be also respectively positioned on its corresponding to open slot rear end extended line on, and the syringe needle hole location set in a row Between corresponding open slot and sample well；Being arranged side by side on the reagent strip pallet has multiple adaptations to be put into reagent strip Open slot；The reagent strip tabletting is distributed on the top surface of reagent strip pallet of open slot two sides；The reagent strip clip is located at On the bottom surface of the reagent strip pallet of opening front of the slot；The heating sheet is mounted on the bottom surface of heating sheet fixed plate, the heating sheet Fixed plate is located at the tail portion of open slot, and is fixed on the lower section of reagent strip pallet, between the top surface of the heating sheet fixed plate is upward Every being provided with multiple heating convex blocks；Optocoupler assembly is respectively positioned on support up and down for the magnet assemblies, magnet assemblies power mechanism and magnet It tries to get to the heart of a matter between plate and reagent strip pallet, and magnet assemblies are located at the lower section of heating sheet fixed plate and heating sheet；The magnet assemblies Power mechanism is for rising or falling magnet assemblies, to complete the magnetic bead separation step to reagent strip；Optocoupler above and below the magnet Component is mounted between magnet assemblies and magnet assemblies power mechanism, locating in tray mold group ontology for detecting magnet assemblies Position.

4. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 3, it is characterised in that: the magnet Component includes magnet fixing seat, multiple big magnet, two guide posts and two guide pillar springs；The bottom of two guide posts is fixed on tray substrate On, the magnet fixing seat is sleeved on two guide posts via corresponding linear bearing and can move up and down, and two guide pillar springs cover respectively It on two guide posts, and withstands between magnet fixing seat and heating sheet fixed plate, is moved down for auxiliary magnet fixing seat；Institute There is the equal perpendicular separation of big magnet to be arranged in magnet fixing seat, and be in its corresponding to open slot rear end extended line On, the top of each big magnet is crossed to form the conical surface by two inclined top faces along the length direction of open slot；Corresponding, two add The magnet via hole that the corresponding big magnet of adaptation passes through is provided in heating sheet fixed plate between hot convex block.

5. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 4, it is characterised in that: the magnet Component power mechanism includes motor mounting plate, DC brushless motor, eccentric disc and bearing and its connecting shaft；The motor mounting plate It is vertically mounted on tray substrate, and the motor mounting plate is big facing towards magnet fixing seat；The DC brushless motor is lateral It is fixed on motor mounting plate, and the output shaft of the DC brushless motor passes through motor mounting plate towards magnet fixing seat；It is described Eccentric disc is connected on the output shaft of DC brushless motor, and the bearing and its connecting shaft are fixed in the disk of eccentric disc, and Deviate the output shaft of DC brushless motor in fixed position；The magnet fixing seat is horizontal towards the side of magnet assemblies power mechanism To adaptation bearing and its slotted hole that rolls in it of connecting shaft is provided with, for stirring under the driving of DC brushless motor Magnet fixing seat drives big magnet to rise or fall.

6. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 1, it is characterised in that: the puncture Liquid relief mould group includes puncturing liquid relief stepper motor, puncture liquid relief synchronous belt component, puncture liquid relief screw drive component, liquid relief to move back pipe Component, punctures liquid relief guide rail slide block component and big sliding panel at withdraw of the needle head selection component；The liquid relief moves back tube assembly and the choosing of withdraw of the needle head It selects component and is installed in the positive middle and lower part of big sliding panel；The back side of the big sliding panel is sliding by screw and puncture liquid relief guide rail Sliding block in block assembly is connected, and two guide rails punctured in liquid relief guide rail slide block component are fixed on by screw interval vertical The front of rack；The back side for puncturing liquid relief stepper motor and being vertically mounted on rack via corresponding fixing piece, and the puncture The output shaft of liquid relief stepper motor is arranged upward, and synchronous with one in puncture liquid relief synchronous belt component via corresponding bearing Belt wheel is connected；The screw rod punctured in liquid relief screw drive component is also vertically mounted on via corresponding bearing and fixing piece The back side of rack, and the upper end of the screw rod via corresponding bearing and punctures another synchronous pulley in liquid relief synchronous belt component It is connected；The nut punctured in liquid relief screw drive component is mounted on the back side of big sliding panel via corresponding fixing piece, And the nut is located in the longitudinal central axis line of the big sliding panel；The synchronous belt punctured in liquid relief synchronous belt component is sleeved on On the two synchronous pulleys, for puncture liquid relief stepper motor driving under drive screw rod rotation, and by with the screw rod phase The nut of cooperation drives the sliding block at big sliding panel and its back side to slide up and down along guide rail, thus related to be mounted on big sliding panel front Liquid relief move back tube assembly and the withdraw of the needle head selection component integrally move up and down.

7. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 6, it is characterised in that: the liquid relief Moving back tube assembly includes that liquid relief moves back pipe motor fixing plate, liquid relief moves back pipe screw motor, liquid relief moves back pipe guide rail slide block component, small sliding Plate, syringe push plate, liquid relief are moved back pipe motor nut, multiple puncture needles, syringe fixed plate, more liquid relief syringes and optocoupler and are passed Sensor；The liquid relief moves back the front that pipe motor fixing plate is laterally vertically fixed on big sliding panel, and the liquid relief moves back pipe screw motor Liquid relief is mounted on to move back on pipe motor fixing plate, and the liquid relief move back pipe screw motor screw rod pass through liquid relief move back pipe motor fixing plate hang down Directly it is arranged downward；The liquid relief move back two guide rails in pipe guide rail slide block component by screw perpendicular separation be fixed on liquid relief move back pipe electricity Big sliding panel front below machine fixed plate, the back side and the liquid relief of the small sliding panel move back the sliding block phase in pipe guide rail slide block component Connection, the syringe push plate are fixed on the top of small sliding panel, and the liquid relief, which moves back pipe motor nut and is mounted on the syringe, to be pushed away On plate, and the screw rod for moving back pipe screw motor with liquid relief matches；Multiple puncture needle perpendicular separations are fixed on the bottom surface of small sliding panel On；The syringe fixed plate is located at the lower section that liquid relief moves back pipe motor fixing plate, and moves back pipe motor via two root posts and liquid relief Fixed plate is connected；More liquid relief syringe perpendicular separations are mounted on the top surface of syringe fixed plate, the syringe push plate Front along being provided with multiple adapters in the card slot of more liquid relief syringe tail portions；The optocoupler sensor is via corresponding solid Determine part be connected to liquid relief move back below pipe motor fixing plate big sliding panel front.

8. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 6, it is characterised in that: the withdraw of the needle Head assembly includes multiple withdraw of the needle headpins, multiple withdraw of the needle plates, withdraw of the needle head stepper motor, withdraw of the needle head synchronous belt component and withdraw of the needle head guide rail Slide block assembly；Wherein, two are installed via corresponding linear bearing in the syringe fixed plate at each liquid relief syringe rear Withdraw of the needle headpin is set with a spring in the upper half of each withdraw of the needle headpin, and the lower end of the two withdraw of the needle headpins is all connected with one piece Withdraw of the needle plate, the front end of the withdraw of the needle plate are provided with the notch that an adaptation returns liquid relief injector head syringe needle；The withdraw of the needle head stepping Motor is connected to the side of small sliding panel, and the output shaft warp of the withdraw of the needle head stepper motor via corresponding fixing piece lateral inward It is connected by corresponding bearing with a synchronous pulley in withdraw of the needle head synchronous belt component, in the withdraw of the needle head synchronous belt component Another synchronous pulley is connected to the other side of small sliding panel via corresponding bearing and connecting shaft；The withdraw of the needle head guide rail slide block Guide rail in component is secured transverse to the positive lower end of small sliding panel by screw, the cunning in the withdraw of the needle head guide rail slide block component Block is connected via corresponding connector with the synchronous belt in withdraw of the needle head synchronous belt component, and to be sleeved on withdraw of the needle head same for the synchronous belt Step is on two synchronous pulleys in component.

9. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 1, it is characterised in that: the darkroom Door gear includes dark-room door, two dark-room door guide rail slide block components and dark-room door power mechanism；The back side of the dark-room door is set respectively It is equipped with the groove and sliding block connecting hole of adaptation two dark-room door guide rail slide block components of insertion, the dark-room door is by screw across these cunnings The guide rail that block connecting hole is connected on the sliding block in dark-room door guide rail slide block component, in the dark-room door guide rail slide block component passes through Screw is vertically fixed on the front of rack；The side of the dark-room door is via corresponding dark-room door connector and dark-room door engine Structure is connected；The dark-room door power mechanism is by dark-room door stepper motor, aforementioned dark-room door connector, dark-room door synchronous belt group Part, dark-room door power mechanism mounting assembly composition；Synchronous belt in the dark-room door synchronous belt component is vertically positioned at dark-room door Side, aforementioned dark-room door connector are connected by screw between synchronous belt and dark-room door；It is described dark in the upper end of the synchronous belt Room door stepper motor is fixed on the front of rack via the dark-room door stepper motor support in dark-room door power mechanism mounting assembly, A synchronous pulley in the dark-room door synchronous belt component is connected to the output of dark-room door stepper motor via corresponding bearing On axis；Synchronous belt support in the lower end of the synchronous belt, the dark-room door power mechanism mounting assembly is being fixed on rack just Face, another synchronous pulley in the dark-room door synchronous belt component are connected to the synchronous belt via corresponding bearing and connecting shaft On support；The synchronous belt is sleeved on the two synchronous pulleys.

10. Full-automatic magnetic beads time-resolved fluorescence immunoassay instrument according to claim 1, it is characterised in that: the reading Value mould group is made of readings X-axis power mechanism, readings Y-axis power mechanism, lens assembly and bar code device；The readings X-axis power Mechanism and readings Y-axis power mechanism be respectively used in the horizontal plane along the x axis with Y direction moving lens component, and read Value X-axis power mechanism is also used in the horizontal plane related Mobile barcode device while moving lens component along the x axis；The mirror Head assembly is used to carry out effective light-emitting data acquisition to being placed on the Reagent Tube for needing readings in tray mold group on multiple reagent strips, The bar code device is used to read the bar code information on multiple reagent strips；

The readings X-axis power mechanism includes readings X-axis stepper motor, readings X-axis synchronous wheel assembly, readings X-axis guide rail sliding block Component and readings X-axis fixed plate；The readings X-axis fixed plate is erected on the bottom plate of rack via two root posts；The X-axis step It is mounted on the vertical plate front of rack along the y axis via corresponding fixing piece into motor, and the output shaft of the X-axis stepper motor is worn It crosses the vertical plate and is connected via a synchronous pulley in corresponding bearing wheel assembly synchronous with readings X-axis, the readings X-axis Another synchronous pulley in synchronous wheel assembly is carried on the back via the vertical plate that corresponding bearing, connecting shaft and fixing piece are connected to rack Face；Guide rail in the readings X-axis guide rail slide block assembly is fixed on the reading between this two synchronous pulley by screw along the x axis It is worth in X-axis fixed plate, the bottom surface of readings Y-axis fixed plate one end in the readings Y-axis power mechanism and readings X-axis guide rail sliding block Sliding block in component is connected, and the top surface of the readings Y-axis fixed plate one end is via corresponding connector and readings X-axis synchronizing wheel Synchronous belt in component is connected；

The readings Y-axis power mechanism includes readings y-axis stepper motor, readings Y-axis synchronous wheel assembly, readings Y-axis guide rail sliding block Component, aforementioned readings Y-axis fixed plate, readings Y-axis idler wheel and readings Y-axis support plate；Wherein, the readings Y-axis support plate also passes through It is erected on the bottom plate of rack by two root posts, the readings Y-axis idler wheel is mounted on the other end of readings Y-axis fixed plate；The Y Shaft step motor is mounted on the idler wheel end of readings Y-axis fixed plate, and the Y-axis stepping electricity via corresponding fixing piece along the x axis The output shaft of machine is connected via a synchronous pulley in corresponding bearing wheel assembly synchronous with readings Y-axis, the readings Y-axis Another synchronous pulley in synchronous wheel assembly is fixed on readings Y-axis fixed plate via corresponding bearing, connecting shaft and fixing piece The other end；Guide rail in the readings Y-axis guide rail slide block assembly by screw be fixed on along the y axis this two synchronous pulley it Between readings Y-axis fixed plate on, the lens assembly is connected with the sliding block in readings Y-axis guide rail slide block assembly, and the camera lens Synchronous belt in component wheel assembly synchronous with readings Y-axis is connected；

The lens assembly includes photomultiplier tube, direct-connected guide-lighting projection seat and direct-connected guide-lighting dimple；The photomultiplier tube installation In direct-connected guide-lighting projection seat, the direct-connected guide-lighting projection seat is mounted on direct-connected guide-lighting dimple；The direct-connected guide-lighting projection seat with it is aforementioned Synchronous belt in the synchronous wheel assembly of readings Y-axis is connected, the cunning in the direct-connected guide-lighting dimple and readings Y-axis guide rail slide block assembly Block is connected；

The bar code device is fixed in readings Y-axis fixed plate via corresponding fixing piece.