TWI756585B - Biochemical reactor, stirring device and extraction method thereof - Google Patents

Abstract

A biochemical reactor, comprising a rack unit, a stirring device arranged in the rack unit, a transfer seat disposed in the rack unit, and a transfer drive mechanism for driving the transfer seat to move, The stirring device is suitable for driving a stirring sleeve, and includes a lifting module for moving up and down along a height direction, a magnetic bar extending along the height direction and including a bottom magnetic attraction portion, and a rotatable A grab head set on the lifting module, the grab head has a driven screw hole screwed with the magnetic rod, the grab head is suitable for connecting with the stirring sleeve, when the lift module drives the grab When the head moves up and down along the height direction relative to the magnetic bar, the grabbing head can drive the stirring sleeve to move up and down, and can drive the stirring sleeve to rotate synchronously.

Description

Biochemical reactor, stirring device and extraction method thereof

The present invention relates to a device and method for extracting nucleic acid, in particular to a biochemical reactor and its stirring device and extraction method.

As shown in FIG. 1, a conventional nucleic acid purification equipment (Patent No. CN206692665 U of the People's Republic of China) comprises a rack 1, a magnetic sleeve 2 which can be moved up and down on the rack 1, and a number of which are arranged in the rack 1. The magnetic sleeve 3 of the magnetic sleeve 2, a first lifting mechanism 4 for driving the magnetic sleeve 2 to move up and down, a magnetic rod holder 5 that can move up and down on the frame 1, a number of The magnetic bar 6 arranged on the magnetic bar frame 5, a second lifting mechanism 7 for driving the magnetic bar frame 5 to move up and down, and a reagent tank placed on the frame 1 and formed in a matrix arrangement 801 deep well plate 8.

Thereby, the first lifting mechanism 4 can drive the magnetic sleeve frame 2 to drive the magnetic sleeves 3 to extend into the corresponding reagent tanks 801, and perform reciprocating up and down vibration movements, so that the magnetic sleeves 3 are located in the corresponding reagent tanks 801. The reagents (not shown), biological samples (not shown) and magnetic beads (not shown) in the reagent tank 801 can be mixed with each other to extract nucleic acids from the biological samples. will be relative to the corresponding test The reagent tank 801 vibrates up and down, and cannot rotate and stir the reagents, biological samples and magnetic beads in the reagent tank 801, and the mixing effect still needs to be improved.

Therefore, one object of the present invention is to provide a stirring device that can overcome the prior art.

Therefore, the stirring device of the present invention is suitable for driving a stirring sleeve, and the stirring device includes a lifting module, a magnetic bar, and a grasping head.

The lifting module is used to move up and down along a height direction.

The magnetic bar extends along the height direction and includes a bottom magnetic attraction portion.

The grasping head is rotatably arranged on the lifting module and has a driven screw hole screwed with the magnetic rod. The grasping head is suitable for connecting with the stirring sleeve. When the lifting module drives the grasping When the head moves up and down along the height direction relative to the magnetic bar, the grabbing head is suitable for driving the stirring sleeve to move up and down, and is suitable for driving the stirring sleeve to rotate synchronously.

Another object of the present invention is to provide a biochemical reactor capable of overcoming at least one of the disadvantages of the prior art.

The biochemical reactor of the present invention includes a rack unit, the above-mentioned stirring device, a transfer base, and a transfer drive mechanism.

The stirring device is arranged in the rack unit.

The transfer base is movably arranged on the rack unit along a conveying direction.

The transfer driving mechanism is used for driving the transfer base to move along the conveying direction.

Another object of the present invention is to provide an extraction method that overcomes at least one of the disadvantages of the prior art.

The extraction method of the present invention comprises:

(A) Make a stirring sleeve move up and down relative to a reagent tank along a height direction, and rotate relative to the reagent tank synchronously.

(B) relatively moving the stirring sleeve and a magnetic bar coaxial to the stirring sleeve along the height direction until the bottom end of the stirring sleeve is adjacent to the bottom end of the magnetic bar.

(C) lowering the stirring sleeve and the magnetic bar synchronously along the height direction until the bottom end of the stirring sleeve and the bottom end of the magnetic bar are adjacent to the bottom end of the reagent tank.

(D) The stirring sleeve and the magnetic rod are raised synchronously along the height direction until the stirring sleeve and the magnetic rod come out of the reagent tank.

The effect of the present invention is: the present invention utilizes the design that the grabbing head is screwed with the magnetic rod, when the lifting module drives the grabbing head to move up and down relative to the magnetic rod along the height direction, the grabbing head In addition to driving the stirring sleeve to move up and down, it also drives the stirring sleeve to rotate synchronously, so that the stirring sleeve can reciprocate up and down and rotate synchronously to stir a reagent tank filled in a reagent cartridge a try inside The reagent, the number of magnetic beads and the number of biological samples are mixed sufficiently and uniformly to achieve the purpose of homogenization.

100: Biochemical Reactor

10: Rack Unit

11: Base

12: Cross frame

20: Stirring device

30: Transfer seat

40: Transfer drive mechanism

41: rack

42: Pinion

43: Transfer drive motor

50: Lifting module

51: Lift the substrate

52: Bearing

60: Magnet

61: Lead screw body

62: Adapter sleeve

63: Bottom magnetic part

70: Grab Head

71: Driven screw hole

72: Insert flange

80: First drive mechanism

81: The first nut

82: The first screw

83: First drive motor

90: Second drive mechanism

91: Mounting plate

92: Second nut

93: Second screw

94: driven gear

95: drive gear

96: Second drive motor

X: conveying direction

Y: horizontal direction

Z: height direction

200: stirring sleeve

210: Groove

300: Reagent Box

310: Reagent tank

320: Reagents

330: Magnetic beads

340: Biological Samples

410: Steps

420: Steps

430: Steps

440: Steps

1: Rack

2: Magnetic sleeve

3: Magnetic sleeve

4: The first lifting mechanism

5: Magnetic rod holder

6: Magnetic bar

7: The second lifting mechanism

8: Deep well plate

801: Reagent tank

Other features and effects of the present invention will be clearly presented in the embodiments with reference to the drawings, wherein: Fig. 1 is a side view of a conventional nucleic acid purification device; Fig. 2 is an embodiment of a biochemical reactor of the present invention Figure 3 is a side view of the embodiment after removing part of the rack; Figure 4 is a side view of a stirring device of the embodiment. An incomplete exploded perspective view of the lifting module, a magnet bar, a grab head and a stirring sleeve; Fig. 5 is a combined cross-sectional view of this embodiment; Fig. 6 is taken along the line VI-VI in Fig. 5 Fig. 7 is an incomplete combined sectional view similar to Fig. 5, illustrating that a reagent tank where a reagent cartridge is moved to its most front end is located under one of the stirring sleeves; Fig. 8 is a similar to Fig. 7 , illustrating that the grabbing head, one of the stirring sleeves installed on the grabbing head and the magnetic rod are lowered synchronously, so that the one of the stirring sleeves and the magnetic rod enter the reagent tank at the forefront; FIG. 9 is a view similar to FIG. 8 , illustrating that the grabbing head and one of the stirring sleeves reciprocate up and down relative to the magnetic bar in a height direction, and at the same time, the grabbing head also moves relative to the magnetic bar Generate rotation, and drive one of the stirring sleeves to generate synchronous rotation; FIG. 10 is a view similar to FIG. 9, illustrating that the grabbing head and the one of the stirring sleeves move upward relative to the magnetic bar along the height direction to the The bottom end of one of the stirring sleeves is adjacent to a bottom magnetic attraction portion of the magnet bar; FIG. 11 is a view similar to FIG. 10 , illustrating that the grabbing head, one of the stirring sleeves and the magnet bar are lowered to the bottom synchronously. The bottom end of a stirring sleeve and the bottom magnetic attraction part of the magnetic bar are adjacent to the bottom end of the reagent tank at the foremost end; FIG. 12 is a view similar to FIG. 11 , illustrating the grabbing head, one of the stirring sleeves and the The magnetic bar rises synchronously to the bottom end of one of the stirring sleeves and the bottom magnetic attraction part of the magnetic bar is out of the reagent tank at the front end, and is located above the one of the reagent cartridges; and FIG. 13 is a schematic flow diagram, An embodiment of the extraction method using the biochemical reactor of the present invention will be described.

Referring to FIGS. 2 , 3 and 4 , it is an embodiment of the biochemical reactor 100 of the present invention. In this embodiment, the biochemical reactor 100 can be used in conjunction with four stirring sleeves 200 and four reagent cartridges 300 . There is a ring-shaped insert groove 210, and each reagent cartridge 300 has several reagent grooves 310. It can be understood that in other variations of this embodiment, In another example, the number of the stirring sleeves 200 and the reagent boxes 300 can also be one, two, three or more than four.

As shown in FIGS. 2 , 3 and 4 , the biochemical reactor 100 includes a rack unit 10 , a stirring device 20 , a transfer base 30 , and a transfer drive mechanism 40 .

The rack unit 10 includes a base 11 and a straddle 12 disposed on the base 11 .

The stirring device 20 is disposed on the cross frame 12 of the rack unit 10 .

The transfer base 30 is movably disposed on the base 11 of the rack unit 10 along a conveying direction X. In this embodiment, the reagent cartridges 300 are detachably installed on the transfer base 30 .

The transfer drive mechanism 40 is used to drive the transfer base 30 to move along the conveying direction X. In this embodiment, the transfer drive mechanism 40 includes a bottom side of the transfer base 30 and along the conveying direction An X-extended rack 41 , a pinion 42 meshing with the rack 41 , and a transfer drive motor 43 disposed on the bottom side of the base 11 of the rack unit 10 and used to drive the pinion 42 .

As shown in FIGS. 4 , 5 and 6 , the stirring device 20 is suitable for driving the stirring sleeves 200 , and the stirring device 20 includes a lifting module 50 , four magnetic bars 60 , four grasping heads 70 , and a first driving mechanism 80, and a second drive mechanism 90. In this embodiment, the magnetic bars 60 and the grabbing heads 70 are arranged at intervals in a horizontal direction Y, and the number of the magnetic bars 60 and the grabbing heads 70 corresponds to the number of the stirring sleeves 200 . number, understandable However, in other variations of this embodiment, the number of the magnetic rods 60 and the grabbing heads 70 can also be one, two, three or more than four.

The elevating module 50 is used to move up and down along a height direction Z. In this embodiment, the elevating module 50 includes a elevating base plate 51 and four sets between the elevating base plate 51 and the grabbing heads 70 . There are bearings 52 between, and the bearings 52 are arranged at intervals in the lateral direction Y. In addition, the number of the bearings 52 corresponds to the number of the grabbing heads 70. It can be understood that other changes in this embodiment are For example, the number of the bearings 52 can also be one, two, three or more than four.

Each magnet bar 60 extends along the height direction Z, and includes a lead screw body 61 , an adapter sleeve 62 disposed at the bottom end of the lead screw body 61 , and a bottom magnetic attraction portion 63 disposed on the adapter sleeve 62 . , in this embodiment, the bottom magnetic attraction part 63 is a magnet.

The grabbing heads 70 are rotatably disposed on the lifting base plate 51 of the lifting module 50 , each grabbing head 70 is sleeved with a respective bearing 52 and has a lead screw body with a respective magnet bar 60 61 is screwed to the driven screw hole 71, and an embedded flange 72. In this embodiment, each grabbing head 70 is suitable for connecting with a respective stirring sleeve 200. When the stirring sleeves 200 are respectively installed on the grabbing heads 70, the embedment protrusions of the grabbing heads 70 The edges 72 are respectively detachably engaged with the embedding grooves 210 of the stirring sleeves 200 , so that the stirring sleeves 200 are respectively positioned on the grabbing heads 70 .

The first driving mechanism 80 includes a first nut 81 disposed on the lifting base plate 51 of the lifting module 50 , a first nut 81 parallel to the magnetic bars 60 and screwed with the first nut 81 The connected first screw 82 and a first driving motor 83 disposed on the cross frame 12 and used for driving the first screw 82 . In this embodiment, when the first driving motor 83 drives the first screw 82 to rotate, the first nut 81 is linked with the lifting base plate 51 to drive the grasping heads 70 relative to the guides of the magnet bars 60 . When the screw body 61 moves up and down along the height direction Z, each grab head 70 is suitable for driving the respective stirring sleeves 200 to move up and down, and for driving the respective stirring sleeves 200 to rotate synchronously.

The second driving mechanism 90 includes an installation plate 91 , a second nut 92 disposed on the installation plate 91 , a second screw 93 parallel to the magnet bars 60 and screwed with the second nut 92 , A driven gear 94 coaxially fixed on the top end of the second screw 93 , a driving gear 95 meshing with the driven gear 94 , and a second driving motor 96 for driving the driving gear 95 . In this embodiment, the top ends of the lead screw bodies 61 of the magnet bars 60 are fixedly disposed on the mounting plate 91, and the mounting plate 91 is higher than the lifting base plate 51 of the lifting module 50 in the height direction Z, The second screw 93 is rotatably disposed on the straddle frame 12 . When the second driving motor 96 drives the second screw 93 to rotate, the second nut 92 can be linked with the mounting plate 91 to drive the magnet bars 60 along the The height direction Z moves up and down.

It should be noted that, when using the biochemical reactor 100 to perform nucleic acid extraction operations, the following pre-operations can be performed first, and the following description is based on the same set of mutually matched magnetic rods 60, grab heads 70, The stirring sleeve 200 and the reagent box 300 are used as representative descriptions, but not limited to this: Pre-step 1: As shown in FIGS. 3 and 7 , the transfer drive motor 43 drives the pinion 42 to rotate, and the rack 41 drives the transfer base 30 to move to the reagent cartridge 300 along the conveying direction X The frontmost reagent tank 310 of the reagent box 300 is located below the stirring sleeve 200, but it is not limited to this. Below the set 200. In this embodiment, all the reagent tanks 310 of the reagent cartridge 300 are respectively filled with reagents 320 with different functions, but only the frontmost reagent tank 310 is further filled with several magnetic beads 330 and several biological samples 340 .

In the second step, as shown in FIG. 8 , the first and second driving motors 83 and 96 are made to rotate the first and second screws 82 and 93 synchronously, so that the first and second nuts 81 and 92 respectively drive the The lifting base plate 51 and the mounting plate 91 descend synchronously along the height direction Z. In this way, the grabbing head 70 , the stirring sleeve 200 installed on the grabbing head 70 and the magnetic bar 60 also descend synchronously, so that the The stirring sleeve 200 enters the reagent tank 310 at the front end.

After completing the pre-steps 1 and 2 of the above-mentioned pre-operation, referring to FIG. 13 , it is an embodiment of the extraction method of the present invention. The extraction method is carried out using the biochemical reactor 100, and includes the following steps:

Step 410 (see FIG. 13 ): As shown in FIG. 9 , the stirring sleeve 200 is moved up and down relative to the frontmost reagent tank 310 along the height direction Z, and synchronously rotates relative to the frontmost reagent tank 310 .

In this embodiment, the first driving motor 83 drives the first screw 82 to rotate, and the first nut 81 is linked with the lifting base plate 51 to drive the grabbing head 70 and the stirring sleeve 200 relative to the magnetic bar The lead screw body 61 of the magnetic bar 60 reciprocates up and down along the height direction Z. During this process, since the driven screw hole 71 of the grabbing head 70 is screwed with the lead screw body 61 of the magnet bar 60, the In addition to driving the stirring sleeve 200 to reciprocate up and down, the grabbing head 70 also rotates relative to the lead screw body 61 to drive the stirring sleeve 200 to rotate synchronously. In this way, the stirring The sleeve 200 moves up and down reciprocatingly and rotates synchronously to stir the reagent 320, the magnetic beads 330 and the biological samples 340 in the reagent tank 310 at the front end with the effect of lysing cells and tissues, so that the The reagents 320, the magnetic beads 330 and the biological samples 340 are sufficiently and uniformly mixed to produce a homogenization effect. During this process, the cell walls and cell membranes of the biological samples 340 will be separated and destroyed, and the contained therein will be released. Nucleic acid, the homogenized biological sample 340 will be attached to the magnetic beads 330 along with the isolated nucleic acid.

Step 420 (see FIG. 13 ): As shown in FIG. 10 , move the stirring sleeve 200 and the magnetic bar 60 coaxial to the stirring sleeve 200 relatively along the height direction Z until the bottom end of the stirring sleeve 200 is adjacent to the magnetic field. The bottom end of the rod 60 preferably moves the stirring sleeve 200 upward along the height direction X until the bottom end of the stirring sleeve 200 is adjacent to the bottom end of the magnetic bar 60 coaxial with the stirring sleeve 200 .

In this embodiment, the first driving motor 83 drives the first screw 82 to rotate, and the first nut 81 is linked with the lifting base plate 51 to drive the grabbing head 70 and the stirring sleeve 200 relative to the magnetic bar The lead screw body 61 of 60 moves upward along the height direction Z until the bottom end of the stirring sleeve 200 is adjacent to the bottom magnetic attraction part 63 of the magnetic bar 60 , at this time, the stirring sleeve 200 will leave the liquid level of the reagent 320 .

Step 430 (see FIG. 13 ): As shown in FIG. 11 , the stirring sleeve 200 and the magnetic bar 60 are lowered synchronously along the height direction Z until the bottom end of the stirring sleeve 200 and the bottom end of the magnetic bar 60 are adjacent to the most The bottom end of the reagent tank 310 at the front end.

In this embodiment, the first and second driving motors 83 and 96 are made to drive the first and second screws 82 and 93 to rotate synchronously, so that the first and second nuts 81 and 92 respectively drive the lifting base plate 51 and the The mounting plate 91 is lowered synchronously along the height direction Z, so that the grabbing head 70 , the stirring sleeve 200 and the magnetic bar 60 are lowered synchronously until the bottom end of the stirring sleeve 200 is adjacent to the bottom magnetic attraction portion 63 of the magnetic bar 60 At the bottom end of the reagent tank 310 at the front end, in this way, the bottom magnetic attraction part 63 can attract the magnetic beads 330 to the outside of the stirring sleeve 200. The pick-up head 70 and the magnetic bar 60 descend synchronously, so the grabbing head 70 will not rotate relative to the lead screw body 61 of the magnetic bar 60 with the stirring sleeve 200. In this way, the stirring sleeve 200 is moving forward In the process of entering the reagent 320 down, that is, the reagent 320 will not be rotated and stirred again.

Step 440 (see FIG. 13 ): As shown in FIG. 12 , make the stirring sleeve 200 and the magnetic bar 60 rise synchronously along the height direction Z to the reagent tank 310 where the stirring sleeve 200 and the magnetic bar 60 come out of the front end .

In this embodiment, the first and second driving motors 83 and 96 are made to drive the first and second screws 82 and 93 to rotate synchronously, so that the first and second nuts 81 and 92 respectively drive the lifting base plate 51 and the The mounting plate 91 rises synchronously along the height direction Z, so that the grabbing head 70, the stirring sleeve 200 and the magnetic bar 60 rise synchronously until the bottom end of the stirring sleeve 200 is separated from the bottom magnetic attraction portion 63 of the magnetic bar 60. The frontmost reagent tank 310 exits and is located above the reagent box 300. During this process, since the grabbing head 70 and the magnet bar 60 are raised synchronously, the grabbing head 70 will not carry The stirring sleeve 200 should rotate relative to the lead screw body 61 of the magnet bar 60, so that the bottom magnetic attraction part 63 can stably attract the magnetic beads 330 to the outside of the stirring sleeve 200. In this way, the magnetic beads 330 can be moved out of the reagent tank 310 at the front end along with the stirring sleeve 200 .

After that, the pre-step 1 of the above-mentioned pre-operation can be performed again, and the other reagent tanks 310 of the reagent cartridge 300 are sequentially moved to be located under the stirring sleeve 200, and the magnetic beads 330 are to be released to other reagents Once inside the tank 310 , the pre-step 2 and steps 410 to 440 of the above-mentioned pre-operation can be repeated, so that the magnetic beads 330 are filled with different reagents 320 having the effect of removing non-nucleic acid substances in other reagent tanks 310 . Repeated cleaning to achieve the purpose of purification and extraction.

Through the above description, the advantages of the present invention can be summarized as follows: Compared with the prior art, the present invention utilizes the design in which the grabbing head 70 is screwed with the magnetic rod 60 , and the first driving mechanism 80 drives the lifting module 50 to drive the grabbing head 70 to face the stirring sleeve 200 . When the lead screw body 61 of the magnet bar 60 reciprocates up and down along the height direction Z, the grabbing head 70 not only drives the stirring sleeve 200 to reciprocate up and down, but also drives the stirring sleeve. 200 generates synchronous rotation, so that the stirring sleeve 200 will reciprocate up and down and rotate synchronously to stir the reagent 320, the magnetic beads 330 and the biological samples 340 in the reagent tank 310, so that the The reagents 320 , the magnetic beads 330 and the biological samples 340 are fully and uniformly mixed, so as to be more homogenized.

It is worth mentioning that, as shown in FIGS. 2 , 3 and 5 , in this embodiment, the transfer base 30 has several detachable temporary storage cylinders 31 , and the stirring sleeves 200 are respectively placed in the In the temporary storage cylinders 31, when the stirring sleeves 200 are to be used, the transfer driving mechanism 40 can first move the transfer base 30 until the stirring sleeves 200 are located between the grabbing heads 70 and the Below the magnetic bar 60, then, the agitating sleeves 200 can be respectively embedded in the grabbing heads 70 by the way that the grabbing heads 70 and the magnetic bars 60 are lowered synchronously, and then, by the Wait for the grabbing head 70 and the magnetic rods 60 to rise synchronously, so that the stirring sleeves 200 are separated from the temporary storage cylinders 31 respectively, so as to facilitate the above-mentioned nucleic acid extraction operation. In this way, after the above-mentioned nucleic acid extraction operation is completed , the transfer drive mechanism 40 can move the transfer base 30 until the temporary storage cylinders 31 are located below the stirring sleeves 200 , and the magnetic rods 60 are lowered relative to the grabbing heads 70 respectively. the square Press the stirring sleeves 200 to push the stirring sleeves 200 away from the grabbing heads 70 and fall into the temporary storage cylinders 31 respectively, so as to facilitate the recovery of the stirring sleeves 200 .

To sum up, the biochemical reactor, the stirring device and the extraction method of the present invention can make the stirring sleeve move up and down reciprocatingly and rotate synchronously to stir the reagent, the magnetic beads and the magnetic beads in the reagent tank. and other biological samples, so that the reagents, the magnetic beads and the biological samples are fully and uniformly mixed, so the object of the present invention can be achieved indeed.

However, the above are only examples of the present invention, and should not limit the scope of the present invention. Any simple equivalent changes and modifications made according to the scope of the application for patent of the present invention and the content of the patent specification are still within the scope of the present invention. within the scope of the invention patent.

100: Biochemical Reactor

10: Rack Unit

11: Base

12: Cross frame

20: Stirring device

30: Transfer seat

31: Temporary storage box

40: Transfer drive mechanism

43: Transfer drive motor

50: Lifting module

51: Lift the substrate

52: Bearing

60: Magnet

61: Lead screw body

62: Adapter sleeve

63: Bottom magnetic part

70: Grab Head

71: Driven screw hole

72: Insert flange

80: First drive mechanism

81: The first nut

82: The first screw

83: First drive motor

90: Second drive mechanism

91: Mounting plate

92: Second nut

93: Second screw

94: driven gear

95: drive gear

96: Second drive motor

X: conveying direction

Y: horizontal direction

Z: height direction

200: stirring sleeve

210: Groove

300: Reagent Box

310: Reagent tank

320: Reagents

330: Magnetic beads

340: Biological Samples

Claims (10)

A stirring device suitable for driving a stirring sleeve, the stirring device comprising: a lift module for moving up and down along a height direction; a magnetic bar extending along the height direction and including a bottom magnetic attraction portion; and A grasping head is rotatably disposed on the lifting module and has a driven screw hole screwed with the magnetic rod. The grasping head is suitable for connecting with the stirring sleeve. When the lifting module drives the grasping When the pick head moves up and down along the height direction relative to the magnetic bar, the grab head is suitable for driving the stirring sleeve to move up and down, and is suitable for driving the stirring sleeve to rotate synchronously. The stirring device according to claim 1, wherein the lifting module comprises a lifting base plate and a bearing disposed between the lifting base plate and the grabbing head. The stirring device according to claim 1, wherein the magnetic bar further comprises a lead screw body screwed into the driven screw hole of the grabbing head, and an adapter sleeve disposed at the bottom end of the lead screw body, the The bottom magnetic attraction part is arranged on the adapter sleeve, and the bottom magnetic attraction part is a kind of magnet. The stirring device according to claim 1, further comprising a first driving mechanism, the first driving mechanism comprising a first nut disposed on the lifting module, and a first nut parallel to the magnetic bar and connected to the first nut The first screw on which the cap is screwed. The stirring device according to claim 4, wherein the first driving mechanism further comprises a first driving motor for driving the first screw. The stirring device according to claim 4, further comprising a second driving mechanism, the second driving mechanism comprising a mounting plate, a second nut disposed on the mounting plate, and a second nut parallel to the magnetic bar and connected to the first The second screw is screwed with two nuts, the magnetic rod is fixedly arranged on the mounting plate, and the mounting plate is higher than the lifting module in the height direction. The stirring device of claim 6, wherein the second driving mechanism further comprises a driven gear coaxially fixed on the second screw, a driving gear meshed with the driven gear, and a drive gear for driving the second screw. A second drive motor for the pinion gear. A biochemical reactor comprising: a rack unit; The stirring device according to any one of claims 1-7 is arranged in the rack unit; a transfer base movably disposed on the rack unit along a conveying direction; and A transfer driving mechanism for driving the transfer base to move along the conveying direction. The biochemical reactor according to claim 8, wherein the transfer driving mechanism comprises a rack disposed on the transfer base and extending along the conveying direction, a pinion engaged with the rack, and a rack disposed on the The rack unit also serves as a transfer drive motor for driving the pinion. An extraction method comprising: (A) make a stirring sleeve move up and down relative to a reagent tank along a height direction, and rotate relative to the reagent tank synchronously; (B) relatively moving the stirring sleeve and a magnetic rod coaxial to the stirring sleeve along the height direction until the bottom end of the stirring sleeve is adjacent to the bottom end of the magnetic rod; (C) lowering the stirring sleeve and the magnetic rod synchronously along the height direction until the bottom end of the stirring sleeve and the bottom end of the magnetic rod are adjacent to the bottom end of the reagent tank; and (D) The stirring sleeve and the magnetic rod are raised synchronously along the height direction until the stirring sleeve and the magnetic rod come out of the reagent tank.

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