CN108277148B - Nucleic acid extraction liquid separation device - Google Patents
Nucleic acid extraction liquid separation device Download PDFInfo
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- CN108277148B CN108277148B CN201810383680.8A CN201810383680A CN108277148B CN 108277148 B CN108277148 B CN 108277148B CN 201810383680 A CN201810383680 A CN 201810383680A CN 108277148 B CN108277148 B CN 108277148B
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- 238000000605 extraction Methods 0.000 title claims abstract description 214
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 75
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 75
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 75
- 239000007788 liquid Substances 0.000 title claims abstract description 67
- 238000000926 separation method Methods 0.000 title claims abstract description 66
- 239000011324 bead Substances 0.000 claims description 31
- 239000003480 eluent Substances 0.000 claims description 11
- 238000002955 isolation Methods 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 24
- 238000002156 mixing Methods 0.000 description 14
- 238000010586 diagram Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000005406 washing Methods 0.000 description 5
- 230000009089 cytolysis Effects 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- 230000006037 cell lysis Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000007886 magnetic bead extraction Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
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- Biotechnology (AREA)
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- Crystallography & Structural Chemistry (AREA)
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- Microbiology (AREA)
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- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a nucleic acid extraction and liquid separation device, and belongs to the technical field of nucleic acid extraction. The extraction plate slides and sets up in the surface of base, is provided with a plurality of extraction holes on the extraction plate, and the extraction part is used for extracting the nucleic acid liquid to the extraction downthehole. The liquid separation part comprises a mounting plate and a liquid separation plate, the mounting plate and the liquid separation plate are both arranged on the surface of the base, and a plurality of liquid separation holes are formed in the liquid separation plate; a plurality of mounting holes are formed in the mounting plate, and transfer pieces are arranged in the mounting holes. The mounting frame is arranged on the base, the transfer frame is arranged on the mounting frame, the first driving part is arranged on the mounting frame and is in driving connection with the transfer frame, the first driving part is used for driving the transfer frame to move above the mounting plate, the extraction plate and the liquid distribution plate, the second driving part is arranged on the transfer frame and is in driving connection with the mounting piece, and the second driving part is used for driving the mounting piece to move up and down and enabling the transfer piece to be selectively mounted on the mounting piece. The nucleic acid extraction and liquid separation device can realize extraction and liquid separation of nucleic acid, and is convenient for subsequent detection of nucleic acid.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction and liquid separation device.
Background
Nucleic acid extraction methods generally comprise several major steps, such as cell lysis, enzyme treatment, separation of nucleic acids from other biological macromolecules, and purification of nucleic acids, each of which may be accomplished by a variety of different methods, alone or in combination.
The nucleic acid extractor is an instrument which automatically completes the extraction work of sample nucleic acid by using matched nucleic acid extraction reagent. In the traditional manual nucleic acid extraction process, a centrifugal clarification method is generally adopted, and the operation is time-consuming and labor-consuming. In recent years, magnetic bead separation technology has appeared, nucleic acid extraction is based on magnetic bead extraction, cell lysate in the magnetic bead method is a protein denaturant, which can cause cell lysis of animals and plants, denature protein bound with DNA, release DNA freely, magnetic beads can specifically adsorb DNA, impurities such as proteins and polysaccharides except DNA are removed by washing, and DNA adsorbed on the magnetic beads is dissociated by eluent to obtain DNA with high purity and concentration, and the method can be used for PCR templates, genetic engineering and the like.
The nucleic acid extractor in the prior art can not realize the process of automatic liquid separation, and has the advantages of more complex operation and low efficiency.
Disclosure of Invention
The invention aims to provide a nucleic acid extraction and liquid separation device which can realize extraction and liquid separation of nucleic acid and improve the working efficiency.
The invention is realized by adopting the following technical scheme:
A nucleic acid extraction and separation device comprising:
A base;
The extraction assembly comprises at least one extraction device, each extraction device comprises an extraction part and an extraction plate, the extraction plates are arranged on the surface of the base in a sliding manner, a plurality of extraction holes are formed in the extraction plates, and the extraction parts are used for extracting nucleic acid liquid into the extraction holes;
the liquid separation part comprises a mounting plate and a liquid separation plate, the mounting plate and the liquid separation plate are arranged on the surface of the base, and a plurality of liquid separation holes are formed in the liquid separation plate; the mounting plate is provided with a plurality of mounting holes, and a transfer piece is arranged in each mounting hole; and
The transfer assembly comprises a transfer frame, a mounting frame, a first driving part, a second driving part and a mounting piece, wherein the mounting frame is arranged on the base, the transfer frame is arranged on the mounting frame, the first driving part is arranged on the mounting frame and in driving connection with the transfer frame and used for driving the transfer frame to move above the mounting plate, the extraction plate and the liquid separation plate, and the second driving part is arranged on the transfer frame and in driving connection with the mounting piece and used for driving the mounting piece to move up and down and enabling the transfer piece to be selectively mounted on the mounting piece.
Further, in a preferred embodiment of the present invention, the transfer assembly further includes a third driving portion, and the mounting frame includes a first vertical rod, a second vertical rod and a cross rod;
The first vertical rod and the second vertical rod are both arranged on the base in a sliding mode, the cross rod is arranged on the first vertical rod and the second vertical rod, the transfer frame is arranged on the cross rod, and the third driving part is connected with the first vertical rod and the second vertical rod in a driving mode and used for driving the installation frame to slide.
Further, in a preferred embodiment of the present invention, the extraction assembly includes a first extraction device and a second extraction device;
The extraction plate of the first extraction device and the extraction plate of the second extraction device are respectively arranged at two ends of the base, and the third driving part is used for driving the mounting frame to slide between the extraction plate of the first extraction device and the extraction plate of the second extraction device.
Further, in a preferred embodiment of the present invention, the extracting portion includes a fourth driving portion, a fifth driving portion, a plurality of magnetic sleeves and a plurality of magnetic bars;
The fourth drive part and the fifth drive part are arranged on the base, each magnetic sleeve and each magnetic rod are located above the extraction plate, one magnetic rod is arranged in one magnetic sleeve in a sliding mode, the fourth drive part is connected with the magnetic rods in a driving mode and used for driving the magnetic rods to move up and down in the magnetic sleeves, and the fifth drive part is connected with the magnetic sleeves in a driving mode and used for driving the magnetic sleeves to move up and down in the extraction holes.
Further, in a preferred embodiment of the present invention, a plurality of rows of extraction holes are provided on the extraction plate, magnetic beads are provided in two rows of extraction holes, the plurality of magnetic sleeves are two rows of magnetic sleeves corresponding to the magnetic beads, and the plurality of magnetic rods are two rows of magnetic rods corresponding to the magnetic sleeves.
Further, in a preferred embodiment of the present invention, each of the extracting devices further includes a sixth driving portion, where the sixth driving portion is in driving connection with the extracting plate to drive the extracting plate to move along a direction from one row of magnetic sleeves to another row of magnetic sleeves.
Further, in a preferred embodiment of the present invention, the extracting device further includes a driving frame, and the fourth driving portion includes a fourth motor, a fourth screw, a fourth nut, and a fourth frame;
The drive frame is arranged on the base, the fourth motor is arranged on the drive frame, an output shaft of the fourth motor is connected with the fourth screw rod in a driving mode, the fourth screw rod is sleeved with a fourth nut, the fourth frame is fixedly connected with the fourth nut, and two rows of magnetic bars are fixed on frame plates, located on two sides of the fourth screw rod, of the fourth frame.
Further, in a preferred embodiment of the present invention, the extracting portion further includes a shielding portion, and the shielding portion is disposed in the plurality of rows of extracting holes and is used for separating the magnetic sleeve from the extracting holes.
Further, in a preferred embodiment of the present invention, the shielding part includes a seventh driving part, an L-shaped shielding plate, and a shielding frame;
The shielding frame is arranged on the driving frame, the seventh driving part is arranged on the shielding frame in a sliding mode, one end of the L-shaped shielding sheet is connected with the seventh driving part, and the other end of the L-shaped shielding sheet is arranged in a plurality of rows of extraction holes and used for separating the magnetic sleeve from the extraction holes.
Further, in a preferred embodiment of the present invention, the nucleic acid isolation and liquid separation apparatus further includes a control system, and the first driving portion, the second driving portion, the fourth driving portion, the fifth driving portion, and the seventh driving portion are all electrically connected to the control system.
The nucleic acid extraction and liquid separation device provided by the preferred embodiment of the invention has the beneficial effects that: firstly, extracting nucleic acid in a sample into an extraction hole through an extraction part, driving a transfer frame to move downwards by a second driving part, enabling a transfer piece in a mounting hole to be mounted into the mounting piece, driving the transfer frame to move upwards by the second driving part, driving the transfer frame to move from the upper part of a mounting plate to the upper part of the extraction plate by a first driving part, driving the transfer frame to move downwards by the second driving part, enabling the transfer piece to enter the extraction hole filled with the nucleic acid, sucking the nucleic acid into the transfer piece, and driving the transfer frame to move upwards by a second motor; the first driving part drives the transfer rack to move from the extraction plate to the liquid separation plate, and the second driving part drives the transfer rack to descend to move the nucleic acid in the transfer piece into the liquid separation hole so as to detect the nucleic acid.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and should not be considered as limiting the scope, and other related drawings can be obtained according to the drawings without inventive effort for a person skilled in the art, which also belong to the protection scope of the present invention.
FIG. 1 is a schematic diagram of a nucleic acid extraction and separation device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of an extraction plate in a nucleic acid extraction and separation apparatus according to an embodiment of the present invention;
FIG. 3 is a schematic view of a base in a nucleic acid extraction and separation apparatus according to an embodiment of the present invention;
FIG. 4 is a first enlarged view at IV of FIG. 1;
fig. 5 is an enlarged view at v in fig. 4;
FIG. 6 is a second enlarged view at IV in FIG. 1;
FIG. 7 is a schematic diagram showing a transfer assembly of a nucleic acid isolation and separation device according to an embodiment of the present invention.
Icon: 100-base; 200-an extraction assembly; 300-transfer assembly; 400-liquid separation part; 500-a first extraction device; 600-a second extraction device; 210-an extraction section; 220-extraction plate; 221-extraction wells; 222-first row; 223-second row; 224-third row; 225-fourth row; 226-fifth row; 227-sixth row; 228-seventh row; 229-eighth row; 231-ninth row; 232-tenth row; 233-eleventh row; 234-twelfth row; 240-sixth driving part; 241-sixth motor; 242-sixth screw rod; 243-sixth nuts; 244-chute; 211-a fourth driving section; 212-a fifth driving part; 213-magnetic bars; 214-magnetic sleeve; 251-fourth motor; 252-fourth screw rod; 253-fourth nut; 254-fourth frame; 261-fifth motor; 262-fifth screw rod; 263-fifth nut; 264-a fifth frame; 270-a shield; 271-seventh driving units; 272-L shaped shielding sheet; 273-a shielding frame; 274-a connector; 275-a shutter; 410-mounting plates; 420-a liquid separation plate; 430-mixing plates; 440-reagent plate; 310-transferring rack; 320-mounting rack; 330-a first driving part; 340-a second drive section.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
Examples
Fig. 1 is a schematic diagram of a nucleic acid extraction and separation apparatus according to the present embodiment. Referring to FIG. 1, in the present embodiment, the nucleic acid extraction and separation apparatus includes a base 100, an extraction module 200, a transfer module 300, and a separation section 400, wherein the base 100 is a mounting base for other components of the nucleic acid extraction apparatus, and the extraction module 200, the transfer module 300, and the separation section 400 are all disposed on the base 100.
The base 100 is a bottom plate having a groove formed therein for mounting the extracting plate 220, the transfer unit 300 and the liquid separating part 400. To allow more samples to be taken, in this embodiment, the extraction assembly 200 includes at least one extraction device, each extraction device including an extraction section 210 and an extraction plate 220. Optionally, the extraction assembly 200 includes two extraction devices, and the two extraction devices are disposed opposite to the base 100. The extraction assembly 200 includes a first extraction device 500 and a second extraction device 600; the extraction plate 220 of the first extraction device 500 and the extraction plate 220 of the second extraction device 600 are disposed at both ends of the base 100, respectively.
Each extracting device is provided with one extracting plate 220, two extracting plates 220 are arranged on the base 100, the structures of the two extracting plates 220 are identical, each extracting plate 220 is slidably arranged on the base 100, each extracting plate 220 is provided with a plurality of extracting holes 221, magnetic beads are selectively arranged in the extracting holes 221, and the extracting part 210 is used for extracting nucleic acid liquid into the extracting holes 221.
Fig. 2 is a schematic structural diagram of an extraction plate 220 in the nucleic acid extraction and separation apparatus according to the present embodiment. Referring to fig. 1 and fig. 2 together, in the present embodiment, a plurality of rows of extraction holes 221 are disposed on the extraction plate 220, and magnetic beads are disposed in at least one row of extraction holes 221. In order to extract more samples at one time, the extraction plate 220 is provided with a plurality of rows of extraction holes 221, and magnetic beads are disposed in two rows of extraction holes 221.
In this embodiment, 12 rows of extraction holes 221 are disposed on the extraction plate 220, wherein the first row 222 and the seventh row 228 of extraction holes 221 are filled with a lysis solution for lysing the sample, the second row 223 and the eighth row 229 of extraction holes 221 are filled with magnetic beads, the third row 224, the fourth row 225, the fifth row 226, the ninth row 231, the tenth row 232 and the eleventh row 233 of extraction holes 221 are filled with different washing solutions for removing impurities such as proteins and polysaccharides in the sample, and the sixth row 227 and the twelfth row 234 of extraction holes 221 are filled with an eluent for eluting nucleic acid samples on the magnetic beads into the eluent.
Fig. 3 is a schematic structural diagram of a base 100 in the nucleic acid isolation and liquid separation apparatus according to the present embodiment. Fig. 3 is a schematic view of the back surface structure of the base 100, referring to fig. 1 and 3, each extracting device further includes a sixth driving portion 240, where the sixth driving portion 240 is disposed on the base 100, and drives the extracting plate 220 to slide on the surface of the base 100 through the sixth driving device.
The sixth driving part 240 includes a sixth motor 241, a sixth screw rod 242, and a sixth nut 243, the sixth motor 241 is disposed in the base 100, a chute 244 is disposed on the surface of the base 100, an output shaft of the sixth motor 241 is in driving connection with the sixth screw rod 242, the sixth nut 243 is sleeved outside the sixth screw rod 242, and a sliding member passing through the chute 244 is disposed on the lower plate surface of the extraction plate 220 and fixedly connected with the sixth nut 243.
When the sixth motor 241 rotates, the sixth screw rod 242 rotates together, and the sixth nut 243 sleeved outside the sixth screw rod 242 cannot rotate along with the sixth screw rod 242 due to the restriction of the sliding groove 244 and the sliding member, the sixth nut 243 moves linearly, and thus the extraction plate 220 connected to the sixth nut 243 through the sliding member slides on the surface of the base 100. By controlling the forward rotation and the reverse rotation of the sixth motor 241, the extraction plate 220 is slid in the opposite direction, that is, the extraction plate 220 is reciprocated in the X-axis direction by the rotation of the sixth motor 241.
The sixth driving parts 240 of the two first extracting devices 500 and the sixth driving parts 240 of the second extracting device 600 are disposed opposite to each other, and drive the extracting plates 220 of the first extracting devices 500 and the extracting plates 220 of the second extracting devices 600 to move relatively or reversely.
FIG. 4 is a first enlarged view at IV of FIG. 1; fig. 5 is an enlarged view of v in fig. 4. Referring to fig. 1-5 together, in the present embodiment, the extracting portion 210 includes a fourth driving portion 211, a fifth driving portion 212, a plurality of magnetic sleeves 214 and a plurality of magnetic rods 213, the fourth driving portion 211 and the fifth driving portion 212 are disposed on the base 100, each of the magnetic sleeves 214 and the magnetic rods 213 is disposed above the extracting plate 220, one magnetic rod 213 is slidably disposed in one magnetic sleeve 214, the fourth driving portion 211 is in driving connection with the magnetic rod 213 for driving the magnetic rod 213 to move up and down in the magnetic sleeve 214, and the fifth driving portion 212 is in driving connection with the magnetic sleeve 214 for driving the magnetic sleeve 214 to move up and down in the extracting hole 221.
The magnetic rod 213 is moved up and down by the fourth driving part 211, the magnetic rod 213 is moved down into the magnetic sleeve 214, and the magnetic sleeve 214 is extended upward; the fifth driving part 212 moves the magnet sleeve 214 up and down, the magnet sleeve 214 moves down into the extraction hole 221, and the magnet sleeve 214 moves up to protrude out of the extraction hole 221.
The magnetic rods 213 and the magnetic sleeves 214 are coaxially arranged, so that one magnetic rod 213 is slidably arranged in one magnetic sleeve 214, the plurality of magnetic sleeves 214 are at least one row of magnetic sleeves 214 corresponding to the magnetic beads, the plurality of magnetic rods 213 are at least one row of magnetic rods 213 corresponding to the magnetic sleeves 214, namely, the plurality of magnetic sleeves 214 form at least one row, and the plurality of magnetic rods 213 form at least one row.
The plurality of magnetic sleeves 214 are two rows of magnetic sleeves 214 corresponding to the magnetic beads, the plurality of magnetic rods 213 are two rows of magnetic rods 213 corresponding to the magnetic sleeves 214, namely, the plurality of magnetic sleeves 214 form two rows of magnetic sleeves 214, the plurality of magnetic rods 213 form two rows of magnetic rods 213, and when one row of magnetic sleeves 214 corresponds to the extraction holes 221 of the twelfth row 234, correspondingly, the other row of magnetic sleeves 214 corresponds to the extraction holes 221 of the sixth row 227. When one row of the magnetic sleeves 214 corresponds to the second row 223 of the extraction holes 221, correspondingly, another row 214 corresponds to the eighth row 229 of the extraction holes 221, and so on.
The sixth driving part 240 is in driving connection with the extracting plate 220, and is used for driving the extracting plate 220 to move along the direction of one row of magnetic sleeves 214 to the other row of magnetic sleeves 214. That is, the extraction plate 220 is reciprocally slid on the chute 244 in the direction of one row of the magnet housings 214 to the other row of the magnet housings 214, that is, reciprocally moved in the X-axis direction by the action of the sixth motor 241, the sixth screw 242, and the sixth nut 243.
The extracting device further includes a driving rack for mounting the fourth driving part 211 and the fifth driving part 212. The fourth driving part 211 includes a fourth motor 251, a fourth screw rod 252, a fourth nut 253, and a fourth frame 254; the drive frame is arranged on the base 100, the fourth motor 251 is arranged on the drive frame, an output shaft of the fourth motor 251 is in drive connection with the fourth screw rod 252, a fourth nut 253 is sleeved outside the fourth screw rod 252, a fourth frame 254 is fixedly connected with the fourth nut 253, and two rows of magnetic bars 213 are fixed on frame plates of the fourth frame 254, which are positioned on two sides of the fourth screw rod 252.
The fourth screw 252 is vertically disposed, and correspondingly, the magnetic rod 213 is also vertically disposed. When the fourth motor 251 rotates, the fourth screw rod 252 rotates together, and the fourth nut 253 fitted around the fourth screw rod 252 cannot rotate along with the fourth screw rod 252 due to the restriction of the fourth frame 254 and the magnetic rod 213, so that the fourth nut 253 moves up and down, and the magnetic rod 213 fixed to the fourth frame 254 moves up and down as the fourth frame 254 moves up and down. By controlling the forward rotation and the reverse rotation of the fourth motor 251, the magnetic rod 213 is thereby moved up and down, i.e., reciprocated in the Z-axis direction.
In this embodiment, the fourth frame 254 includes a first frame and a second frame that are disposed opposite to each other, and a third frame and a fourth frame that are disposed opposite to each other, where the third frame is connected to the fourth nut 253, and a row of magnetic rods 213 are fixed on the first frame and the second frame of the fourth frame 254, respectively, and the magnetic rods 213 move up and down synchronously with the up and down movement of the fourth frame 254, that is, reciprocate along the Z-axis direction.
The fifth driving part 212 includes a fifth motor 261, a fifth screw rod 262, a fifth nut 263, and a fifth frame 264; the fifth motor 261 is arranged on the driving frame, an output shaft of the fifth motor 261 is in driving connection with the fifth screw rod 262, a fifth nut 263 is sleeved outside the fifth screw rod 262, a fifth frame 264 is fixedly connected with the fifth nut 263, and two rows of magnetic sleeves 214 are fixed on frame plates of the fifth frame 264, which are positioned on two sides of the fifth screw rod 262.
Fifth screw 262 is vertically disposed and correspondingly, magnetic sleeve 214 is also vertically disposed. When the fifth motor 261 rotates, the fifth screw rod 262 rotates together, and the fifth nut 263 fitted outside the fifth screw rod 262 cannot rotate along with the fifth screw rod 262 due to the restriction of the fifth frame 264 and the magnetic sheath 214, so that the fifth nut 263 moves up and down, and the fifth frame 264 connected to the fifth nut 263 moves up and down, and the magnetic sheath 214 fixed to the fifth frame 264 also moves up and down. By controlling the forward rotation and the reverse rotation of the fifth motor 261, the magnet sleeve 214 is moved up and down, i.e., reciprocated in the Z-axis direction.
In this embodiment, the fifth frame 264 includes a first frame and a second frame that are disposed opposite to each other, and a third frame and a fourth frame that are disposed opposite to each other, the third frame is connected to the fifth nut 263, and a row of magnetic sleeves 214 are respectively fixed on the first frame and the second frame of the fifth frame 264, and the magnetic sleeves 214 move up and down synchronously with the up and down movement of the fifth frame 264, i.e. reciprocate along the Z-axis direction.
Fig. 6 is a second enlarged view at iv in fig. 1. Referring to fig. 4 and 6 together, the extracting portion 210 further includes a shielding portion 270, where the shielding portion 270 is disposed in the plurality of rows of extracting holes 221 for separating the magnetic sleeve 214 from the extracting holes 221. After the extraction is completed, the extraction plate 220 is moved, so that the magnetic beads on the magnetic sleeve 214 are separated from the extraction holes 221 filled with the eluent by the shielding part 270 in order to prevent the samples on the surfaces of the magnetic beads on the magnetic sleeve 214 from dripping into the extraction holes 221, and the samples in the eluent are prevented from being polluted.
The shielding portion 270 includes a seventh driving portion 271, an L-shaped shielding plate 272 and a shielding frame 273. The shielding frame 273 is disposed on the driving frame, the seventh driving portion 271 is slidably disposed on the shielding frame 273, one end of the l-shaped shielding plate 272 is connected to the seventh driving portion 271, and the other end is disposed on the extraction hole 221 for separating the magnetic sleeve 214 from the extraction hole 221.
By the seventh driving part 271, the L-shaped shielding plate 272 is controlled to be disposed between the two rows of the magnetic sleeves 214 or above the one row of the extraction holes 221, and if the extraction plate 220 moves from the first row 222 toward the direction of the extraction holes 221 of the twelfth row 234, the shielding plate is disposed above the extraction holes 221 of the sixth row 227. If the extraction plate 220 is moved from the twelfth row 234 towards the extraction holes 221 of the first row 222, a shielding plate is provided above the extraction holes 221 of the twelfth row 234, avoiding contamination of the sample in the eluate.
The seventh driving part 271 includes a seventh motor, a seventh screw rod, and a seventh nut; the seventh motor is arranged on the shielding frame 273, an output shaft of the seventh motor is in driving connection with a seventh screw rod, a seventh nut is sleeved outside the seventh screw rod and is in sliding connection with the shielding frame 273, and one end of the L-shaped shielding plate 272 is fixedly connected with the seventh nut.
The seventh screw rod is transversely arranged, when the seventh motor rotates, the seventh screw rod rotates together, and due to the limiting effect of the shielding frame 273, a seventh nut sleeved outside the seventh screw rod cannot rotate along with the seventh screw rod, so that the seventh nut horizontally slides on the shielding frame 273, and an L-shaped shielding plate 272 connected with the seventh nut horizontally slides on the shielding frame 273. The L-shaped shielding plate 272 is reciprocated in the horizontal direction, i.e., in the X-axis direction, by controlling the forward rotation and the reverse rotation of the seventh motor.
The L-shaped shielding plate 272 includes a connecting member 274 and a shielding plate 275, the connecting member 274 and the shielding plate 275 form an L-shaped structure, one end of the connecting member 274 is connected with the seventh nut and the extending direction of the connecting member 274 is identical to the extending direction of the seventh screw rod, the extending direction of the shielding plate 275 is perpendicular to the extending direction of the seventh screw rod, and the extending direction of the shielding plate 275 is identical to the arrangement direction of a row of the magnetic sleeves 214 so as to separate the magnetic sleeves 214 from the extraction holes 221.
After the nucleic acid extraction is completed, in order to facilitate the subsequent separation of the nucleic acid solution, the extraction plate 220 of the first extraction device 500 and the extraction plate 220 of the second extraction device 600 need to be moved to the middle of the base 100, even if the extraction plate 220 of the first extraction device 500 is staggered from the extraction portion 210 of the first extraction device 500, the extraction plate 220 of the second extraction device 600 is staggered from the extraction portion 210 of the second extraction device 600, when the extraction plate 220 is moved, in order to prevent the liquid on the magnetic sleeve 214 from falling into the extraction hole 221 to pollute the nucleic acid solution, the seventh motor needs to be controlled to move the shielding plate 275 below the magnetic sleeve 214 to separate the magnetic sleeve 214 from the extraction hole 221, so as to prevent the nucleic acid solution on the magnetic sleeve 214 from falling into the extraction hole 221 to pollute the nucleic acid solution in the extraction hole 221.
Alternatively, the shielding plate 275 is disposed under the magnetic sleeves 214 outside the first and second extracting devices 500 and 600 when the extracting plate 220 moves toward the middle of the base 100, thereby preventing contamination of the nucleic acid solution in the extracting hole 221.
The liquid separation part 400 comprises a mounting plate 410 and a liquid separation plate 420, wherein the mounting plate 410 and the liquid separation plate 420 are arranged on the surface of the base 100, and a plurality of liquid separation holes are formed in the liquid separation plate 420; the mounting plate 410 is provided with a plurality of mounting holes in which the transfer members are disposed.
The mounting plate 410 is mainly used for arranging mounting members, one mounting plate 410 is provided, a plurality of mounting holes are formed in the mounting plate 410, and transfer members are arranged in the mounting holes. In this embodiment, the transfer member is a tip, and the tip is used for sucking the nucleic acid solution, so that the amount of the nucleic acid solution sucked can be controlled.
The liquid separation part 400 further comprises a mixing plate 430 and a reagent plate 440, wherein a plurality of mixing holes are formed in the mixing plate 430, a plurality of reagent holes are formed in the reagent plate 440, reagent bottles can be inserted into the reagent holes, reagents in the reagent bottles are sucked through tip heads, the reagents are transferred into the mixing holes, and then nucleic acid liquid is placed into the mixing holes, so that the nucleic acid liquid and the reagents are mixed, and a sample to be detected is obtained.
The liquid separation plate 420 is a pcr plate, after the nucleic acid liquid and the reagent are mixed, the mixture is dripped onto the pcr plate through a tip, and then the pcr plate is taken down and placed on a pcr instrument for detecting nucleic acid. In this embodiment, the liquid separation plate 420 includes three pcr plates, and the number of pcr plates can be determined according to the number of samples.
The transfer module transfers the nucleic acid solution extracted by the extraction module 200 into a mixing hole provided on the mixing plate 430, and transfers the reagent in the reagent hole into the mixing hole, so that the reagent is mixed with the nucleic acid solution, and then transferred into a liquid separation hole on the liquid separation plate 420, thereby obtaining the sample to be detected.
Fig. 7 is a schematic diagram of a transfer assembly in the nucleic acid isolation and separation device according to the present embodiment. Referring to fig. 1 and 7 together, in the present embodiment, the transfer assembly includes a transfer frame 310, a mounting frame 320, a first driving portion 330, a second driving portion 340 and a mounting member, the mounting frame 320 is disposed on the base 100, the transfer frame 310 is disposed on the mounting frame 320, the first driving portion 330 is disposed on the mounting frame 320 and is in driving connection with the transfer frame 310, for driving the transfer frame 310 to move above the mounting plate 410, the extraction plate 220 and the liquid separation plate 420, and the second driving portion 340 is disposed on the transfer frame 310 and is in driving connection with the mounting member, for driving the mounting member to move up and down and for selectively mounting the transfer member on the mounting member.
The mounting frame 320 includes a first vertical rod, a second vertical rod, and a cross rod; the first vertical rod and the second vertical rod are both slidably arranged on the base 100, the cross rod is arranged on the first vertical rod and the second vertical rod, and the transferring frame 310 is arranged on the cross rod. The transfer assembly further includes a third driving part for driving the mounting frame 320 to slide between the withdrawing plate 220 of the first withdrawing device 500 and the withdrawing plate 220 of the second withdrawing device 600. That is, the third driving part is drivingly connected to the first vertical rod and the second vertical rod, for driving the mounting frame 320 to slide, that is, the third driving part is used for driving the first vertical rod and the second vertical rod to slide on the base 100, so that the transfer frame 310 fixed on the cross rod slides between the extracting plate 220 of the first extracting device 500 and the extracting plate 220 of the second extracting device 600. That is, the third driving part can slide the first and second vertical bars of the mounting frame 320 on the base 100, and the first and second vertical bars reciprocate from the extracting plate 220 of the first extracting device 500 toward the extracting plate 220 of the second extracting device 600, that is, reciprocate along the X-axis direction.
The third drive portion sets up in base 100, third drive portion includes two, every third drive portion includes third motor, third lead screw and third nut, the output shaft drive of third motor connects the third lead screw, and third motor and third lead screw are located base 100, the third nut cover is located outside the third lead screw, first montant is connected the third nut at a third drive portion, the third nut at another third drive portion is connected to the second montant, the third motor synchronous motion of two third drive portions, thereby make first montant and second montant synchronous motion, reach the purpose at the position of X axle direction removal carriage.
The first drive portion 330 sets up in the horizontal pole of mounting bracket 320, first drive portion 330 is connected with shifting the frame 310 drive, be used for driving shifting the frame 310 reciprocating sliding on the horizontal pole, promptly along the direction motion of Y axle on the horizontal pole, first drive portion 330 includes first motor, first lead screw, first nut, the first lead screw of output shaft drive connection of first motor, the extending direction of first lead screw is unanimous with the extending direction of horizontal pole, first nut cover is located outside the first lead screw, first nut slip sets up in the horizontal pole, shifting the frame 310 and being fixed in first nut, shifting the frame 310 along with first nut synchronous motion.
The second driving part 340 includes a second motor, a second screw rod and a second nut, the second motor is disposed on the transfer frame 310, an output shaft of the second motor is connected with the second screw rod in a driving manner, the second nut is sleeved outside the second screw rod, the mounting member is fixed on the second nut, the second screw rod is vertically disposed, when the second motor rotates, the second nut moves up and down, namely moves along the Z-axis direction, the mounting member connected with the second nut also moves up and down, when the mounting member moves to the lower part and is located on the tip, the mounting member clamps the tip, the tip is disposed on the mounting member, the first motor and the third motor are used for transferring until reaching the purpose, the tip is used for sucking liquid, the liquid in the tip is extruded at the transfer position, the liquid is sucked again at the transfer position, and the like.
The nucleic acid isolation and separation device further includes a control system, and the first driving part 330, the second driving part 340, the fourth driving part 211, the fifth driving part 212, and the seventh driving part 271 are electrically connected to the control system.
Further, the control system is electrically connected with the first motor, the second motor, the third motor, the fourth motor 251, the fifth motor 261, the sixth motor 241 and the seventh motor, and the control system sends out signals and controls the forward rotation and the reverse rotation of the motors through the signals, so that the extraction and the liquid separation of nucleic acid are realized.
The working principle of the nucleic acid extraction and liquid separation device provided by the embodiment is as follows: the first row 222 and the seventh row 228 of extraction holes 221 are filled with lysate, the second row 223 and the eighth row 229 of extraction holes 221 are filled with magnetic beads, the third row 224, the fourth row 225, the fifth row 226, the ninth row 231, the tenth row 232 and the eleventh row 233 of extraction holes 221 are filled with different washing solutions, and the sixth row 227 and the twelfth row 234 of extraction holes 221 are filled with eluent; reagent tubes are inserted into reagent holes in the reagent plate 440, and tip heads are inserted into mounting holes in the mounting plate 410.
The sample is placed in the extraction hole 221 filled with the lysis solution, the fifth motor 261 rotates to enable the magnetic sleeve 214 to move up and down along the Z axis, the mixture of the lysis solution and the sample is vibrated and homogenized, the sample is smashed, and the nucleic acid enters the lysis solution.
The sixth motor 241 is controlled to slide the slide plate along the X-axis, the two rows of magnetic sleeves 214 are opposite to the second row 223 and the eighth row 229 of the extraction holes 221 filled with magnetic beads, the magnetic rods 213 and the magnetic sleeves 214 are controlled to move downwards along the Z-axis by the fourth motor 251 and the fifth motor 261, and the magnetic rods 213 attract the magnetic beads in the second row 223 of the extraction holes 221 and the eighth row 229 of the extraction holes 221 through the magnetic sleeves 214.
The sixth motor 241 is controlled to slide the sliding plate along the X axis, the two rows of magnetic sleeves 214 are opposite to the first row 222 and the seventh row 228 of extraction holes 221 filled with samples, the fourth motor 251 rotates to enable the magnetic rod 213 to move upwards along the Z axis, the fifth motor 261 rotates to enable the magnetic sleeves 214 to move downwards along the Z axis, the attracted magnetic beads enter the first row 222 and the seventh row 228 of extraction holes 221 filled with samples, the fifth motor 261 continues to control the magnetic sleeves 214 to move upwards and downwards along the Z axis to oscillate, and therefore the samples are adhered to the surfaces of the magnetic beads, the fourth motor 251 and the fifth motor 261 control the magnetic rod 213 and the magnetic sleeves 214 to move downwards along the Z axis together, and the magnetic rod 213 attracts the magnetic beads adhered with the samples through the magnetic sleeves 214.
The sixth motor 241 is controlled to slide the extraction plate 220 along the X-axis, the magnetic sleeve 214 attracting the magnetic beads is made to face over the extraction holes 221 of the third row 224 and the ninth row 231, the magnetic rod 213 is made to move upward along the Z-axis by the rotation of the fourth motor 251, the magnetic sleeve 214 is made to move downward along the Z-axis by the rotation of the fifth motor 261, the attracted magnetic beads enter the extraction holes 221 of the third row 224 and the ninth row 231 containing washing liquid, the fifth motor 261 controls the magnetic sleeve 214 to repeatedly move up and down along the Z-axis, proteins, polysaccharides and other impurities in the sample are removed, nucleic acids are left to be adsorbed on the surfaces of the magnetic beads, the magnetic rod 213 and the magnetic sleeve 214 are controlled to move downward along the Z-axis together by the fourth driving part 211 and the fifth driving part 212, the magnetic beads adhered with the sample are made to be attracted by the magnetic rod 213 through the magnetic sleeve 214, and finally the proteins, polysaccharides and other impurities in the sample are removed by washing the samples in the fourth row 225 and the tenth row 232, the fifth row 226 and the eleventh row 233 in sequence.
The sixth motor 241 is controlled to slide the extraction plate 220 along the X axis, so that the magnetic sleeve 214 attracting the magnetic beads is over the extraction holes 221 of the sixth row 227 and the twelfth row 234 containing the eluent, the magnetic rod 213 is moved upwards along the Z axis by the rotation of the fourth motor 251, the magnetic sleeve 214 is moved downwards along the Z axis by the rotation of the fifth motor 261, the attracted magnetic beads enter the extraction holes 221 containing the eluent, the nucleic acid on the magnetic beads enters the eluent to obtain the nucleic acid liquid, and then the magnetic beads are taken out.
The seventh motor is controlled to rotate so that the L-shaped shielding plate 272 moves along the X-axis direction, the shielding plate 275 of the first extraction device 500 shields the outer magnetic sleeve 214 of the first extraction device 500, the shielding plate 275 of the second extraction device 600 shields the outer magnetic sleeve 214 of the second extraction device 600, and the sixth motor 241 is controlled to rotate so that the extraction plate 220 of the first extraction device 500 and the extraction plate 220 of the second extraction device 600 slide in opposite directions along the X-axis, and the two extraction plates 220 are located in the middle of the base 100. In addition, during the sliding process of the two extraction plates 220, the nucleic acid liquid on the magnetic sleeve 214 is prevented from entering the extraction hole 221 filled with the eluent due to the shielding effect of the shielding plate 275, so that the sample is prevented from being polluted.
The second motor is controlled to rotate, the mounting piece moves downwards along the Z axis, the tip head in the mounting hole is mounted in the mounting piece, the second motor reverses to enable the mounting piece to move upwards along the Z axis, the third motor drives the first vertical rod and the second vertical rod to move along the X axis, the transfer frame 310 and the reagent plate 440 are located in the same X axis displacement direction, the first motor controls the transfer frame 310 to slide along the Y axis direction, the transfer frame 310 is enabled to move to the upper side of the reagent plate 440, the second motor is controlled to rotate, the mounting piece moves downwards along the Z axis, the tip head in the mounting piece absorbs reagents in the reagent bottle, and the second motor reverses to enable the mounting piece to move upwards along the Z axis. The rotation of the third motor and the first motor is continued to move the transfer frame 310 to above the mixing plate 430, the second motor is controlled to squeeze the reagents from the tips into the mixing holes, and the tips on the mounting member are removed.
Continuing to use the above mode to take the new tip, the third motor rotates to enable the transfer frame 310 and the extraction plate 220 to be located in the same X-axis displacement direction, the first motor controls the transfer frame 310 to slide along the Y-axis direction to enable the transfer frame 310 to move to the upper portion of the extraction plate 220, the second motor is controlled to rotate, the mounting piece moves downwards along the Z-axis, the tip in the mounting hole absorbs the nucleic acid liquid in the extraction hole 221, and the second motor reverses to enable the mounting piece to move upwards along the Z-axis. The rotation of the third motor and the first motor is continuously controlled to move the transfer frame 310 to the upper side of the mixing plate 430, and the second motor is controlled to squeeze the nucleic acid solution in the tip into the mixing hole, and mix the nucleic acid solution with the reagent in the mixing hole.
And continuously taking the new tip by using the mode, controlling the rotation of the third motor, the first motor and the second motor, and enabling the tip to absorb the mixed liquid in the mixed hole and transfer the mixed liquid into the liquid distribution hole of the pcr plate to obtain a sample to be detected, so that the pcr instrument is used for detecting the sample subsequently.
The nucleic acid extraction and liquid separation device realizes the operations of automatic extraction and purification of nucleic acid and construction of a PCR polymerase chain reaction system, replaces manpower and liberates manpower; meanwhile, the biological hazard faced by operators is reduced, the pretreatment of PCR detection can be safely, rapidly, accurately and stably realized, and the cost is low, and the PCR detection can be directly carried out after the pretreatment is finished. The PCR detection method has extremely important significance in the aspects of rapidly diagnosing bacterial infectious diseases clinically and the like.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A nucleic acid extraction and separation device, comprising:
A base;
the extraction assembly comprises at least one extraction device, each extraction device comprises an extraction part and an extraction plate, the extraction plates are arranged on the surface of the base in a sliding manner, and a plurality of extraction holes are formed in the extraction plates, and the extraction parts are used for extracting nucleic acid liquid into the extraction holes; the fourth driving part and the fifth driving part are arranged on the base, each magnetic sleeve and each magnetic rod are positioned above the extraction plate, one magnetic rod is arranged in one magnetic sleeve in a sliding mode, the fourth driving part is connected with the magnetic rods in a driving mode and used for driving the magnetic rods to move up and down in the magnetic sleeves, and the fifth driving part is connected with the magnetic sleeves in a driving mode and used for driving the magnetic sleeves to move up and down in the extraction holes; the extraction device also comprises a driving frame; a plurality of rows of extraction holes are formed in the extraction plate, magnetic beads are arranged in two rows of extraction holes, and eluent is arranged in two rows of extraction holes; the magnetic sleeves are two rows of magnetic sleeves corresponding to the magnetic beads, and the magnetic rods are two rows of magnetic rods corresponding to the magnetic sleeves;
the liquid separation part comprises a mounting plate and a liquid separation plate, the mounting plate and the liquid separation plate are both arranged on the surface of the base, and a plurality of liquid separation holes are formed in the liquid separation plate; the mounting plate is provided with a plurality of mounting holes, and a transfer piece is arranged in each mounting hole;
The transfer assembly comprises a transfer frame, a mounting frame, a first driving part, a second driving part and a mounting piece, wherein the mounting frame is arranged on the base, the transfer frame is arranged on the mounting frame, the first driving part is arranged on the mounting frame and is in driving connection with the transfer frame and is used for driving the transfer frame to move above the mounting plate, the extraction plate and the liquid separation plate, and the second driving part is arranged on the transfer frame and is in driving connection with the mounting piece and is used for driving the mounting piece to move up and down and enabling the transfer piece to be selectively mounted on the mounting piece; each extracting device further comprises a sixth driving part which is in driving connection with the extracting plate and used for driving the extracting plate to move along the direction of one row of the magnetic sleeves to the other row of the magnetic sleeves; the extracting part further comprises shielding parts, wherein the shielding parts are arranged in a plurality of rows of extracting holes and are used for separating the magnetic sleeve from the extracting holes; the shielding part comprises a seventh driving part, an L-shaped shielding sheet and a shielding frame; the shielding frame is arranged on the driving frame, the seventh driving part is arranged on the shielding frame in a sliding way, one end of the L-shaped shielding piece is connected with the seventh driving part, and the other end of the L-shaped shielding piece is arranged in a plurality of rows of extraction holes and used for separating the magnetic sleeve from the extraction holes; the seventh driving part comprises a seventh motor, a seventh screw rod and a seventh nut, the seventh motor is arranged on the shielding frame, an output shaft of the seventh motor is in driving connection with the seventh screw rod, the seventh nut is sleeved outside the seventh screw rod and is in sliding connection with the shielding frame, and one end of the L-shaped shielding piece is fixedly connected with the seventh nut; the seventh driving part is used for controlling the L-shaped shielding sheet to be arranged between two rows of magnetic sleeves or above one row of extraction holes, and the L-shaped shielding sheet is used for controlling the forward rotation and the reverse rotation of the seventh motor to enable the L-shaped shielding sheet to reciprocate in the horizontal direction.
2. The nucleic acid isolation dispensing apparatus of claim 1, wherein the transfer assembly further comprises a third drive portion, the mounting bracket comprising a first vertical bar, a second vertical bar, and a cross bar;
The first vertical rod and the second vertical rod are both arranged on the base in a sliding mode, the cross rod is arranged on the first vertical rod and the second vertical rod, the transfer rack is arranged on the cross rod, and the third driving part is connected with the first vertical rod and the second vertical rod in a driving mode and is used for driving the installation frame to slide.
3. The nucleic acid extraction and separation apparatus of claim 2, wherein the extraction assembly comprises a first extraction device and a second extraction device;
The first extraction device the extraction plate with the second extraction device the extraction plate set up respectively in the both ends of base, third drive portion is used for the drive the mounting bracket slide between the first extraction device the extraction plate with the second extraction device the extraction plate.
4. The nucleic acid isolation and separation apparatus according to claim 3, wherein the fourth driving section includes a fourth motor, a fourth screw, a fourth nut, and a fourth frame;
The driving frame is arranged on the base, the fourth motor is arranged on the driving frame, an output shaft of the fourth motor is in driving connection with the fourth screw rod, a fourth nut is sleeved outside the fourth screw rod, a fourth frame is fixedly connected with the fourth nut, and two rows of magnetic bars are fixed on frame plates of the fourth frame, wherein the frame plates are positioned on two sides of the fourth screw rod.
5. The nucleic acid isolation and separation apparatus according to claim 4, further comprising a control system, wherein the first driving unit, the second driving unit, the fourth driving unit, the fifth driving unit, and the seventh driving unit are electrically connected to the control system.
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