CN103789198B - Full automatic instrument for extracting nucleic acid - Google Patents
Full automatic instrument for extracting nucleic acid Download PDFInfo
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- CN103789198B CN103789198B CN201410070377.4A CN201410070377A CN103789198B CN 103789198 B CN103789198 B CN 103789198B CN 201410070377 A CN201410070377 A CN 201410070377A CN 103789198 B CN103789198 B CN 103789198B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
Present invention is disclosed a kind of Full automatic instrument for extracting nucleic acid, for realizing from sample message collection, sample automatic filling, consumptive material automatic loading, leaching process is monitored automatically, the full-automation that PCR reaction system is set up, comprise workplatform, and the sample adding system be arranged on described workplatform, deep-well plates running gear, nucleic acid-extracting apparatus, described workplatform also arranges a plurality of work station, described work station comprises sample area, reagent area and extraction product storage area, described sample adding system is given described deep-well plates running gear respectively from sample area and reagent area and extracts product storage area load sample and PCR reaction reagent, sample is transplanted on below described nucleic acid-extracting apparatus by described deep-well plates running gear, carry out the nucleic acid extraction reaction of sample, the extraction product that reaction is formed leaves described extraction product storage area in.The present invention effectively reduces the impact of human factor on experimental result, improves purity and the efficiency of nucleic acid extraction.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, especially relate to a kind of Full automatic instrument for extracting nucleic acid achieved from sample message collection, sample automatic filling, consumptive material automatic loading, leaching process are monitored automatically, PCR reaction system is set up full-automatic operation.
Background technology
Nucleic acid (RNA (ribonucleic acid), thymus nucleic acid DNA) be the elementary cell representing life entity hereditary feature, detection of nucleic acids carries out advanced biological detection on a molecular scale, compare traditional morphologic detection, cytology detects, immunology detection etc. have more susceptibility, specificity, without remarkable advantages such as window phases, detection of nucleic acids is recognized as a kind of gold standard method of standard gradually in recent years, be widely used in hepatitis, the serious infectious diseases such as AIDS and SARS, influenza, emerging infectious disease diagnosis and detections such as hand foot mouth disease and be widely used in biology, medical research.Detection of nucleic acids comprises qualitative PCR (Polymerase Chain Reaction, polymerase chain reaction), molecular hybridization, the technology such as real-time fluorescence quantitative PCR (Real time QPCR), the primary key of these nucleic acid detection techniques will complete to extract the nucleic acid of biological specimen, therefore extracts the nucleic acid-templated prerequisite becoming follow-up detection of nucleic acids efficiently and accurately.
Beads enrichment extractive technique uses the surface of nanotechnology to superparamagnetic nano particle improve and after finishing, be prepared into superparamagnetism monox nanometer magnetic bead.This magnetic bead can identify specifically with nucleic acid molecule and efficiently be combined on micro interface.Utilize the superparamagnetism of monox nanometer microballoon, under the effect of externally-applied magnetic field, can separate from DNA and RNA blood, animal tissues, food, pathogenic micro-organism equal samples, it has the feature such as simple, quick, accurate and obtains the generally accreditation of people, has become the Main Means of current clinical nucleic acid extraction.
Beads enrichment extractive technique also has some limitations, and its uncertainty extracting result comes from the impact of experimental implementation and reagent system.Along with the continuous progress of reagent phase level, stability, the consistence of extraction element also progressively improve, and the principal element affecting nucleic acid extraction result is exactly experimental implementation.Current inspection body takes artificial experimental implementation to coordinate the mode of semi-automatic extraction device mostly, and extraction element only has single magnetic bead locomotive function, and the pre-treatment of sample, reagent all has operator manually to complete with the work such as to be separated of extracting product.There is individual difference in manual operation, not only affects the accuracy of result, also limit the flux of detection when sample size is various.Automation experiment flow operations can be provided, high-throughout nucleic acid extracting instrument becomes an important topic faced by people.
Summary of the invention
The object of the invention is to the defect overcoming prior art, a kind of Full automatic instrument for extracting nucleic acid is provided, by setting up nucleic acid extraction reaction system and PCR reaction system, achieve the full-automatic operation of nucleic acid extraction process, effectively reduce the impact of human factor on experimental result, improve purity and the efficiency of nucleic acid extraction.
For achieving the above object, the present invention proposes following technical scheme: a kind of Full automatic instrument for extracting nucleic acid, comprise workplatform, and the sample adding system be arranged on described workplatform, deep-well plates running gear, nucleic acid-extracting apparatus, described workplatform also arranges a plurality of work station, described work station comprises sample area, reagent area and extraction product storage area, described sample adding system is given described deep-well plates running gear respectively from sample area and reagent area and extracts product storage area load sample and PCR reaction reagent, sample is transplanted on below described nucleic acid-extracting apparatus by described deep-well plates running gear, carry out the nucleic acid extraction reaction of sample, the extraction product that reaction is formed leaves described extraction product storage area in.
Preferably, the sample adding device that described sample adding system comprises transverse moving device, the longitudinal motion device vertical with described transverse moving device and is arranged on described longitudinal motion device, described transverse moving device coordinates with described longitudinal motion device and drives described sample adding device to sample to relevant work station.
Described transverse moving device comprises horizontal drive motor, laterally driving slide rail and laterally drives slide block, described longitudinal motion device laterally drives slide block to be connected with described, and described longitudinal motion device carries out traverse motion with described sample adding device along the described slide rail that laterally drives under the driving of described horizontal drive motor.
Described longitudinal motion device comprises vertical drive motor, zigzag tread patterns slide rail and zigzag tread patterns slide block, described sample adding device is connected with described zigzag tread patterns slide block, and described zigzag tread patterns slide block carries out longitudinal to-and-fro movement with described sample adding device along described zigzag tread patterns slide rail under the driving of described vertical drive motor.
Described sample adding device comprises automatic sampling gun, the sample loading gun vertical driving mechanism be connected with described automatic oil gun and sample loading gun switch, and described in described sample loading gun on-off control, sample loading gun vertical driving mechanism drives described automatic sampling gun to move up and down.
The end of described automatic sampling gun is provided with sliding sleeve, for unloading used disposable rifle head.
Described sample loading gun vertical driving mechanism comprises pressurization rifle drive-motor, the transmission rig being arranged on the buffer gear on described automatic sampling gun and being connected with described pressurization rifle drive-motor, described pressurization rifle drive-motor drives described transmission rig to move up and down with described automatic sampling gun.
The bar code scan module that described sample area comprises sample loading module and is connected with described sample loading module, described sample loading module comprises sample loading base, is arranged on a plurality of sample pipe supports on described sample loading base and inserts the sample tube be loaded on described sample pipe support, described bar code scan module scans the sample tube on described sample pipe support one by one, records the information of each sample tube.
Described bar code scan module comprises sample rack haulage gear, be arranged on the barcode scanner of described sample rack haulage gear side, and the translational drive mechanism that be connected vertical with described sample rack haulage gear, described sample rack haulage gear drags whole piece sample pipe support and moves back and forth along the direction parallel with described barcode scanner, described translational drive mechanism promotes the sample pipe support place that described sample rack haulage gear moves to respective scanned, scans one by one to sample tube.
Described work station also comprises rifle head storage area and scrap concrete district, described rifle head storage area is mounted with the disposable rifle head of application of sample, described scrap concrete district comprises waste liquid recovery apparatus and discarded rifle head retrieving arrangement, is respectively used to collect the waste liquid and used rifle head that produce in nucleic acid extraction process.
Described reagent area comprises reagent and preserves module and pre-mixing cup position, described reagent preserves that module comprises low temperature inner core, the temperature control unit be arranged on described low temperature inner core, cover insulation cover on described low temperature inner core and a plurality of reagent bottle, and described reagent bottle leaves in-core in described low temperature in.
Each described reagent bottle is integral structure, comprise Reagent Tube support and be encapsulated in a plurality of Reagent Tubes on described Reagent Tube support, the in-built reagent carrying different components of different described Reagent Tubes, during use, is placed into in-core in low temperature and carries out cryopreservation by reagent bottle entirety.
On described insulation cover, corresponding reagent bottle is provided with plural number row well, and described automatic sampling gun draws reagent through described well from described reagent bottle.
Described deep-well plates running gear comprises deep-well plates actuator, the deep-well plates locating device be flexibly connected with described deep-well plates actuator, the deep-well plates be arranged on described deep-well plates locating device, and described deep-well plates actuator drags described deep-well plates locating device entirety and moves to below described nucleic acid-extracting apparatus.
Described deep-well plates is inserted and is loaded with plural number stirring cover, the described upper surface stirring cover is provided with hook, be convenient to stir and overlap running gear to the unloading of stirring cover.
Described extraction product storage area is placed with microwell plate, described microwell plate is provided with a plurality of taper hole, for loading the product after extraction.
Described workplatform also comprises consumptive material drawer, described rifle head storage area, reagent area, deep-well plates running gear, extraction product storage area and scrap concrete district are installed on described consumptive material drawer, make these work stations can with the pull-out of consumptive material drawer entirety, be convenient to the timely replacing that sample, reagent, rifle are first-class.
Described nucleic acid-extracting apparatus comprises bar magnet running gear, be connected with described bar magnet running gear and drive its bar magnet drive-motor carrying out moving up and down, stir and overlap running gear, overlap running gear and be connected with described stirring and drive its stirring carrying out moving up and down to overlap drive-motor.
Described workplatform also comprises aerosol proofing unit, described aerosol proofing unit comprises discharge cell, ion deposition unit, conduction filter membrane and detecting unit, described discharge cell electric discharge ionized gaseous molecules generates gaseous ion, described gaseous ion is combined with aerosol particle, described ion deposition unit removes the too much gaseous ion that ionization produces, charged aerosol particle intercepts by described conduction filter membrane, described detecting unit detects the quantity of electric charge of the charged aerosol particle of described intercepting, obtain concentration and the size information of described aerosol particle, when the concentration of aerosol particle reaches certain value, just report to the police in time, ensure that accuracy and the validity of nucleic acid extraction result.
Compared with prior art, beneficial effect of the present invention is:
1, the sample message collection in nucleic acid extraction process can be completed automatically, sample automatic filling, consumptive material automatic loading, leaching process are monitored automatically, PCR reaction system is set up, to avoid in leaching process manual operation to the interference of test-results, enhance the validity and accuracy of extracting result, improve the efficiency of nucleic acid extraction.
2, the overall auto-translating of deep-well plates running gear that the present invention takes extremely extracts the scheme in district, compared with shifting the technology of deep-well plates with existing employing mechanical arm, more succinct reliable.
3, the present invention arranges reagent and preserves module in reagent area, has the function of low-temperature storage reagent, extends the duration of service of reagent, ensure that the functional quality of reagent.
4, the reagent bottle that the present invention adopts is the encapsulating structure of integral type, and structure is simple, be convenient to processing.
5, nucleic acid extracting reagent of the present invention adopts pre-packaged form to preserve, and the mode that existing full-automatic nucleic acid extraction instrument all adopts complete machine to be annotated by syringe imbibition, the solution of the present invention has effectively simplified the complexity of reagent filling operation, improves the reliability of complete machine and the accuracy of reagent filling result.
6, the present invention adds the spillage of aerosol proofing unit for reaction solution gas molecule in timely Monitoring equipment working process, warning can be sent when reaching finite concentration, effective monitoring nucleic acid extraction process, makes the confidence level of nucleic acid extraction result be further enhanced.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of Full automatic instrument for extracting nucleic acid of the present invention;
Fig. 2 is the schematic layout pattern of workplatform of the present invention;
Fig. 3 is the structural representation of sample adding system of the present invention;
Fig. 4 is the structural representation of sample adding device in Fig. 3;
Fig. 5 is the structural representation of sample area of the present invention;
Fig. 6 is the structural representation of reagent area of the present invention;
Fig. 7 is the structural representation in scrap concrete district of the present invention;
Fig. 8 is the structural representation of deep-well plates running gear of the present invention;
Fig. 9 is the structural representation of nucleic acid-extracting apparatus of the present invention;
Figure 10 is the structural representation that the present invention stirs cover crawl.
Embodiment
Below in conjunction with accompanying drawing of the present invention, clear, complete description is carried out to the technical scheme of the embodiment of the present invention.
Disclosed a kind of Full automatic instrument for extracting nucleic acid, for realizing the fully automated of nucleic acid extraction process and PCR reaction, described extraction apparatus comprises operative body and computer for controlling (not shown), described computer for controlling, for carrying out the acquisition process etc. of sample data information, realizes the operation controlling and monitor described operative body.Composition graphs 1, shown in Fig. 2, described operative body comprises casing assembly, and the workplatform 1 be arranged in described casing assembly, sample adding system 2, deep-well plates running gear 16, nucleic acid-extracting apparatus 18, aerosol proofing unit 4, described workplatform 1 is also provided with a plurality of work station, described work station comprises sample area 11, reagent area 12, rifle head storage area (13, 14), scrap concrete district 15 and extraction product storage area 17, these work stations are for loading the sample reagent needed for nucleic acid extraction process, PCR reaction reagent, disposable rifle is first-class.
Described casing assembly is used for playing protective effect to each several part in described operative body, as shown in Figure 1, the shield cap that described casing assembly comprises frame 38 and is encapsulated in outside described frame 38, described shield cap comprises main shield cap 31, side shield cap (32, 33), drawer panel (34, 35), sample area panel 36 and rear defence guard shield 37, described main shield cap 31, side shield cap (32, 33) and rear defence guard shield (34, 35) other faces in frame 38 except bottom surface are encapsulated, described drawer panel (34, 35) be connected with described work station, described sample area panel 36 is arranged on described main shield cap 31, for encapsulating described sample area 11, the structure design of certain described casing assembly is not limited to the structure of above-mentioned introduction, as long as the encapsulation of operative body and the integral drawing of work station can be realized.
Described sample adding system 2 is for realizing the automatic delivery of sample, PCR reaction reagent, extraction product etc.Shown in composition graphs 2 and Fig. 3, described sample adding system 2 comprises transverse moving device 21, longitudinal motion device 22 and sample adding device 23, described longitudinal motion device 22 is connected with described transverse moving device 21 is vertical, described sample adding device 23 is arranged on described longitudinal motion device 22, and described transverse moving device 21 coordinates with described longitudinal motion device 22 and controls described sample adding device 23 and run to each work station place.
Shown in composition graphs 2 and Fig. 3, described transverse moving device 21 comprises laterally driving slide rail 212, and drive slide block 213 with the described horizontal drive motor 211 laterally driving slide rail 212 to be connected with horizontal, the described slide block 213 that laterally drives is connected with described longitudinal motion device 22, and described horizontal drive motor 211 drives the described slide block 213 that laterally drives to drive described sample adding device 23 to carry out horizontal slip along the described slide rail 212 that laterally drives.Described longitudinal motion device 22 comprises vertical drive motor 221, zigzag tread patterns slide rail 222 and zigzag tread patterns slide block 223, described sample adding device 23 is connected with described zigzag tread patterns slide block 223, and described vertical drive motor 221 drives described zigzag tread patterns slide block 223 to drive described sample adding device 23 to carry out wiping action along described zigzag tread patterns slide rail 223.
Described laterally driving on slide rail 212 and described zigzag tread patterns slide rail 222 is provided with screw mandrel (not shown), and vertical or horizontal drive-motor drives it to drive the screw mandrel on slide rail to realize driving the motion of slide block accordingly.
As shown in Figure 4, described sample adding device 23 comprises automatic sampling gun 231, sample loading gun vertical driving mechanism 232 and sample loading gun switch 237, described automatic sampling gun 231 inside is provided with vacuum unit (not shown), described automatic sampling gun 231 realizes the suction of liquid by described vacuum unit, the lower end of described automatic sampling gun 231 is provided with sliding sleeve 233, and described sliding sleeve 233 is for realizing the automatic drawing unloading of rifle head.
Described sample loading gun vertical driving mechanism 232 comprises sample loading gun drive-motor 234, the transmission rig 235 be connected with described pressurization rifle drive-motor 234, the buffer gear 236 be arranged on described automatic sampling gun 231, and described pressurization rifle drive-motor 234 drives described transmission rig 235 to move up and down with described automatic sampling gun 231.
Described sample loading gun switch 237 controls described sample loading gun vertical driving mechanism 232 and drives described automatic sampling gun 231 to move up and down.With the transverse moving device 21 in sample adding system 2 be X-axis, longitudinal motion device 22 for Y-axis and sample adding device 23 for Y-axis sets up system of coordinates, described transverse moving device 21, longitudinal motion device 22 and sample adding device 23 drive described automatic sampling gun 231 to move on X, the Y, Z-direction of system of coordinates respectively.
Described sample area 11 is for load sample reagent and the collection realizing sample information, and described sample reagent can adopt blood or tissue juice sample.As shown in Figure 5, described sample area 11 comprises sample loading module and bar code scan module, described sample loading module comprises a plurality of sample pipe supports 111, sample tube 112 and sample loading base 113, described sample pipe support 111 is movably connected on described sample loading base 113, described sample pipe support 111 arranges plurality of sample pore, and described sample tube is placed in described sample pore.Sample tube 112 in the embodiment of the present invention adopts Φ 13 × 100mm standard heparin tube, and the equal label in its side, for the scanning of bar code scan module.
Described bar code scan module comprises sample barcode scanner 114, sample rack haulage gear 115, translational drive mechanism 116, described sample barcode scanner 114 is arranged on the side of described sample rack haulage gear 115, described sample rack haulage gear 115 is connected with described sample pipe support 111, be parallel to described sample barcode scanner 114 for driving described sample pipe support 111 to move back and forth, described translational drive mechanism 116 is connected with described sample rack haulage gear 115, for promoting sample pipe support 111 place of described sample rack haulage gear 115 to respective scanned, one by one sample tube 112 is scanned.
Described sample loading module and bar code scan module have cooperatively interacted the autoscan of sample reagent, particularly, during bar code scan, described sample rack haulage gear 115 drags sample pipe support 111 to-and-fro movement, sample barcode scanner 114 records the bar code information of each sample tube 112, after first sample pipe support 111 has scanned, translational drive mechanism 116 promotes sample rack haulage gear 115 integral translation to a second sample pipe support 111 and scans, by that analogy until the last item sample pipe support 111 all completes scanning.
Described reagent area 12 is for loading the reagent needed for PCR reaction, as shown in Figure 6, described reagent area 12 comprises reagent and preserves module 121 and pre-mixing cup position 122, described reagent preserves module 121 for cryopreservation PCR reaction reagent, it comprises temperature control unit 123, low temperature inner core 124 and insulation cover 125, described temperature control unit 123 is arranged on described low temperature inner core 124, for regulating the temperature of described low temperature inner core 124, under making it to be in the suitable low temperature environment of preservation reagent place, described low temperature inner core 124 is provided with a plurality of reagent bottle chamber, the reagent bottle 126 holding PCR reaction reagent is mounted with in described reagent bottle chamber, described insulation cover 125 covers the upper end of described heat insulation inner core 124, certain heat insulation effect is played to described low temperature inner core 124, on described insulation cover 125, corresponding reagent bottle 126 is provided with plural number row well 127, described automatic sampling gun 231 draws reagent through described well 237 from described reagent bottle 126.
Unlike the prior art, reagent bottle 126 in the present invention is designed to integral structure, the a plurality of Reagent Tubes 129 comprising Reagent Tube support 128 and be encapsulated on described Reagent Tube support 128, the in-built PCR reaction reagent carrying different components of different described Reagent Tubes 129.Reagent bottle 126 in the embodiment of the present invention is that Dispoable medical polypropylene material is injection molded, in-built described PCR reaction reagent mainly comprises two kinds: reaction internal reference reagent and PCR react premix reagent, during use, reagent bottle 126 entirety is put into low temperature inner core 124 cryopreservation.
Described rifle head storage area is for depositing the disposable rifle head needed for extraction, according to actual needs, can multiple rifle head storage area be set on described workplatform 1, the present embodiment is to arrange two rifle head storage areas so that the structure of workplatform 1 in the present invention to be described, composition graphs 2, shown in Fig. 7, described rifle head storage area comprises the first rifle head storage area 13 and the second rifle head storage area 14, for the ease of changing rifle head in time, described first rifle head storage area 13 is installed on a consumptive material drawer 141 with reagent area 12, described consumptive material drawer 141 realizes the integral drawing of described rifle head storage area.
The waste that described scrap concrete district produces for reclaiming nucleic acid extraction process, shown in composition graphs 2 and Fig. 7, described scrap concrete district 15 comprises waste liquid recovery apparatus 151 and discarded rifle head retrieving arrangement 152, be respectively used to collect the waste liquid and used disposable rifle head that produce in nucleic acid extraction process, two devices are all by the container (not shown) below scrap concrete to workplatform.
Described extraction product storage area 17 is for depositing the product after extraction, shown in composition graphs 1 and Fig. 7, described extraction product storage area 17 is placed with microwell plate 171, microwell plate 171 in the embodiment of the present invention adopts Dispoable medical polypropylene material injection molded, be made up of (8 row × 12 arrange) 96 taper holes, volume has 0.1ml, 0.2ml two kinds of specifications available.
Described deep-well plates running gear 16 is for load sample and drive sample to arrive the below of described nucleic acid-extracting apparatus 18, carries out the automatic extraction of nucleic acid.As shown in Figure 8, described deep-well plates running gear 16 comprises deep-well plates actuator 161, deep-well plates actuator 162 and deep-well plates 164, described deep-well plates 164 is positioned on described deep-well plates actuator 161, cover 163 is stirred for placing, described deep-well plates actuator 162 is connected with described deep-well plates actuator 161, move back and forth between starting position and described nucleic acid-extracting apparatus 18 lower position for dragging described deep-well plates actuator 161, during use, stirring cover 163 inserts after in deep-well plates 164 by operator, deep-well plates 164 is arranged on deep-well plates locating structure 161, described deep-well plates actuator 162 can drag the abstraction reaction carrying out nucleic acid below deep-well plates locating structure 161 to nucleic acid-extracting apparatus 18.
Described deep-well plates locating structure 161 is also equipped with heating unit (not shown), can heats described deep-well plates 164.
Deep-well plates 164 in the embodiment of the present invention adopts Dispoable medical polypropylene material injection molded, is made up of (8 row × 12 arrange), and is circular arc camber at the bottom of hole 96 square holes.
Described nucleic acid-extracting apparatus 18 is for carrying out nucleic acid extraction reaction process, described process comprises the cracking of sample, washing and wash-out, as shown in Figure 9, described nucleic acid-extracting apparatus 18 comprises bar magnet running gear 181, stir cover running gear 182, bar magnet drive-motor 183 and stirring cover drive-motor 184, described bar magnet drive-motor 183 is connected with described bar magnet running gear 181 and drives described bar magnet fitness machine 181 to pump, described stirring cover drive-motor 184 overlaps running gear 182 be connected with described stirring, and drive described stirring cover running gear 182 to pump.
Unlike the prior art, the present invention has added aerosol proofing unit 4 on instrument, ionized gaseous molecules is utilized to make electric charge on aerosol particle band, there is between the average band electricity of aerosol particle and particle diameter the exponential relationship determined, the concentration of particle and the principle of size information just can be obtained by the average band electricity detecting particle, the spillage of reaction solution gas molecule in timely detecting instrument working process, can warning be sent when reaching finite concentration, improve the safety in utilization of instrument and the accuracy of experimental result.Described aerosol proofing unit 4 comprises discharge cell, ion deposition unit, conduction filter membrane and detecting unit, its specific works principle is: described discharge cell electric discharge ionized gaseous molecules, make to ionize the gaseous ion generated to be combined with aerosol particle, thus make aerosol particle charged; After aerosol particle is charged, through ion deposition unit ion deposition, remove the too much gaseous ion because ionization produces, avoid them to have an impact to ensuing measurement; Finally charged aerosol particle is dammed on described conduction filter membrane, detected the quantity of electric charge on aerosol particle by detecting unit, draw concentration and the size information of aerosol particle through data inversion.
The specific works flow process of Full automatic instrument for extracting nucleic acid of the present invention is as follows:
Before instrument work, first will complete following preparation work: operator loads the specific bit of deep-well plates 164 by stirring cover 163, pull-out consumptive material drawer 141, is arranged on deep-well plates 164 on deep-well plates locating structure 161; Reagent bottle 126 is arranged in the low temperature inner core 124 of reagent area 12, loads standard 20ml cryopreservation tube for reagent premix in pre-mixing cup position 122 simultaneously; Sample pipe support 111 is loaded sample area 11 one by one; The disposable rifle head of the first rifle head storage area 13 and the second rifle head storage area 14 is supplemented complete; Product storage area 17 is extracted by being placed on without the microwell plate 171 used.
After preparation work completes, then start and extract flow process as follows: first sample pipe support 111 is scanned one by one, record the bar code information of each sample tube 112; Simultaneously, below described deep-well plates running gear 16 integral translation to described nucleic acid-extracting apparatus 18, as shown in Figure 10, after described stirring cover running gear 182 moves downward and taken out from deep-well plates 164 by stirring cover, described deep-well plates running gear 16 moves to starting position.
Secondly, automatic sampling gun 231 is under the control of transverse moving device 21, longitudinal motion device 22, sample adding device 23, move to after the first rifle head storage area 13 loads disposable rifle head, move to sample area 11 again and draw sample, afterwards sample is delivered to deep-well plates 164 stock layout basis, finally move to discarded rifle head retrieving arrangement 152, by disposable rifle head unloading, automatic sampling gun 231 repeats said process, until sample is all added into deep-well plates 164.
Then, described deep-well plates running gear 16 moves to below nucleic acid-extracting apparatus 18, bar magnet running gear 181 pumps, carry out the automatic extraction of nucleic acid, while nucleic acid extraction, described automatic sampling gun 231 moves to the first rifle head storage area 13 and loads disposable rifle head, move to reagent area 12 again and inhale reagent, then move to extraction product storage area 17 and arrange reagent, after completing reagent filling, automatic sampling gun 231 moves unloading rifle head to discarding rifle head retrieving arrangement 152.
Subsequently, the bar magnet running gear 181 on described nucleic acid-extracting apparatus 18 overlaps running gear 182 relative movement with stirring, is offloaded in deep-well plates by stirring cover 163, and after stirring cover 163 has unloaded, described deep-well plates running gear 16 returns to starting position.
Afterwards, described automatic sampling gun 231 moves to the second rifle head storage area 14 and loads disposable rifle head, then moves to deep-well plates 164 suction extraction product, then moves to and extracts product storage area 17 row extraction product, finally move to discarded rifle head retrieving arrangement 152, by disposable rifle head unloading.
Finally, described automatic sampling gun 231 moves to the first rifle head storage area 13 and loads disposable rifle head, move to deep-well plates 164 again and inhale waste reaction solution, then waste liquid recovery apparatus 151 removal waste fluid is moved to, after the waste liquid repeated in aforesaid operations to all deep-well plates 164 sucks, move to discarded rifle head retrieving arrangement 152 again, disposable rifle head unloads by the sliding sleeve 233 of automatic sampling gun 231 end, and whole nucleic acid extraction flow process terminates.
Technology contents of the present invention and technical characteristic have disclosed as above; but those of ordinary skill in the art still may do all replacement and the modification that do not deviate from spirit of the present invention based on teaching of the present invention and announcement; therefore; scope should be not limited to the content that embodiment discloses; and various do not deviate from replacement of the present invention and modification should be comprised, and contained by present patent application claim.
Claims (6)
1. a Full automatic instrument for extracting nucleic acid, it is characterized in that, comprise casing assembly, the workplatform be arranged in described casing assembly, a plurality of work stations be arranged on described workplatform, sample adding system, deep-well plates running gear, nucleic acid-extracting apparatus and aerosol proofing unit
Described work station comprises sample area, reagent area and extracts product storage area;
Described sample area comprises sample loading module and bar code scan module, described sample loading module comprises a plurality of sample pipe supports, sample tube and sample loading base, described sample pipe support is movably connected on described sample loading base, described sample pipe support arranges plurality of sample pore, and described sample tube is placed in described sample pore, the equal label in described sample tube side, for the scanning of bar code scan module, described bar code scan module comprises sample barcode scanner, sample rack haulage gear, translational drive mechanism, described sample barcode scanner is arranged on the side of described sample rack haulage gear, described sample rack haulage gear is connected with described sample pipe support, be parallel to described sample barcode scanner for driving described sample pipe support to move back and forth, described translational drive mechanism is connected with described sample rack haulage gear, for promoting the sample pipe support place of described sample rack haulage gear to respective scanned, one by one sample tube is scanned,
Described casing assembly comprises frame and is encapsulated in the shield cap outside described frame, described shield cap comprises main shield cap, side shield cap, drawer panel, sample area panel and rear defence guard shield, other faces in frame except bottom surface encapsulate by described main shield cap, side shield cap and rear defence guard shield, described drawer panel is connected with described work station, described sample area panel is arranged on described main shield cap, for encapsulating described sample area;
Described sample adding system comprises transverse moving device, longitudinal motion device and sample adding device, described longitudinal motion device is connected with described transverse moving device is vertical, described sample adding device is arranged on described longitudinal motion device, and described transverse moving device coordinates with described longitudinal motion device and controls described sample adding device and run to each work station place;
Described sample adding device comprises automatic sampling gun, sample loading gun vertical driving mechanism and sample loading gun switch, described automatic sampling gun inside is provided with vacuum unit, described automatic sampling gun realizes the suction of liquid by described vacuum unit, the lower end of described automatic sampling gun is provided with sliding sleeve, and described sliding sleeve is for realizing the automatic drawing unloading of rifle head; Described sample loading gun vertical driving mechanism comprises sample loading gun drive-motor, the transmission rig be connected with described sample loading gun drive-motor, the buffer gear be arranged on described automatic sampling gun, and described sample loading gun drive-motor drives described transmission rig to move up and down with described automatic sampling gun; Described in described sample loading gun on-off control, sample loading gun vertical driving mechanism drives described automatic sampling gun to move up and down, and described transverse moving device, longitudinal motion device and sample adding device drive described automatic sampling gun to move on X, the Y, Z-direction of system of coordinates respectively;
Described deep-well plates running gear comprises deep-well plates actuator, deep-well plates locating structure and deep-well plates, described deep-well plates is positioned on described deep-well plates locating structure, cover is stirred for placing, described deep-well plates actuator is connected with described deep-well plates locating structure, move back and forth between starting position and described nucleic acid-extracting apparatus lower position for dragging described deep-well plates locating structure, described deep-well plates locating structure is also equipped with heating unit, described deep-well plates is heated;
Described nucleic acid-extracting apparatus comprises bar magnet running gear, stirs cover running gear, bar magnet drive-motor and stir cover drive-motor, described bar magnet drive-motor is connected with described bar magnet running gear and drives described bar magnet fitness machine to pump, described stirring cover drive-motor overlaps running gear be connected with described stirring, and drives described stirring cover running gear to pump;
Described aerosol proofing unit comprises discharge cell, ion deposition unit, conduction filter membrane and detecting unit, described discharge cell electric discharge ionized gaseous molecules generates gaseous ion, described gaseous ion is combined with aerosol particle, described ion deposition unit removes the too much gaseous ion that ionization produces, charged aerosol particle intercepts by described conduction filter membrane, described detecting unit detects the quantity of electric charge of the charged aerosol particle of described intercepting, obtains concentration and the size information of described aerosol particle.
2. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, described reagent area comprises reagent and preserves module and pre-mixing cup position, described reagent preserves that module comprises low temperature inner core, the temperature control unit be arranged on described low temperature inner core, cover insulation cover on described low temperature inner core and a plurality of reagent bottle, and described reagent bottle leaves in-core in described low temperature in.
3. Full automatic instrument for extracting nucleic acid according to claim 2, it is characterized in that, each described reagent bottle is integral structure, comprises Reagent Tube support and is encapsulated in a plurality of Reagent Tubes on described Reagent Tube support, the in-built reagent carrying different components of different described Reagent Tube.
4. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, described work station also comprises rifle head storage area and scrap concrete district, described rifle head storage area is mounted with the disposable rifle head of application of sample, described scrap concrete district comprises waste liquid recovery apparatus and discarded rifle head retrieving arrangement, is respectively used to collect the waste liquid and used rifle head that produce in nucleic acid extraction process.
5. Full automatic instrument for extracting nucleic acid according to claim 4, it is characterized in that, described workplatform also comprises consumptive material drawer, and described rifle head storage area, reagent area, deep-well plates running gear, extraction product storage area and scrap concrete district are installed on described consumptive material drawer.
6. Full automatic instrument for extracting nucleic acid according to claim 1, is characterized in that, described extraction product storage area is placed with microwell plate, described microwell plate is provided with multiple taper hole.
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