CN201201943Y - Nucleic acid extracting system apparatus - Google Patents
Nucleic acid extracting system apparatus Download PDFInfo
- Publication number
- CN201201943Y CN201201943Y CN 200820057014 CN200820057014U CN201201943Y CN 201201943 Y CN201201943 Y CN 201201943Y CN 200820057014 CN200820057014 CN 200820057014 CN 200820057014 U CN200820057014 U CN 200820057014U CN 201201943 Y CN201201943 Y CN 201201943Y
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- liquid
- nucleic acid
- test tube
- separatory
- acid extraction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a nucleic acid extraction system, wherein a liquid division gun is connected with a test tube, the reagent is divided by the liquid division gun into eight flows of same flux to flow into eight test tubes via eight pipes, to be heated by a water bath and homogenized by a swing bed, and the effective components of the tissue fluid are absorbed on the magnetic balls and the residuals are waste liquid, the tubes are arranged on a test tube frame, the Tip tube is connected with a liquid transfer gun, the test tube is arranged with magnetic balls, two sides of the eight tube frame are both provided with magnets for absorbing the magnetic balls, thereby realizing liquid transfer. After using the liquid transfer gun to transfer the liquid, the effective biological tissue components on the magnetic balls are left in the test tubes, the waste liquid is poured into a liquid bottle via a discharge system. The nucleic acid extraction system can effectively separate liquid and accurately confirm effective nucleic acid extraction, and has high speed and simple structure.
Description
Technical field
The utility model relates to a kind of nucleic acid extraction system and device.
Background technology
Various nucleic acid extraction system all can be divided into DNA extraction system and RNA extraction system.Mainly be DNA and the RNA that isolates in the reagent, be used for heredity test/gene type, individual recognition, identify the species and the place of production, the genome analysis of different plant species, the virus and fundamental research and the expression analysis (RT-PCR) that identify to infect, isolated viral RNA etc. from virus infected cell.
Domestic manufacturing enterprise of nucleic acid extraction system is being investigated the back discovery, most product form is more single, though be suitable for several samples (whole blood, tissue, culturing cell and plasmid), and have high quality and reclaim productive rate, automatic and type hand operating system are arranged, but tolerance range not very high.Our nucleic acid extraction system is separatory, moves liquid, extracts the device that waste liquid combines, pointed nucleic acid extraction function, and simple and easy to do, the precision height.
Summary of the invention
The utility model is to propose a kind of HY-1 type nucleic acid extraction system and device at the existing nucleic acid extraction not high deficiency of system's tolerance range, this device can extract the biological tissue that needs effectively, can guarantee the validity of extraction from accuracy and accuracy two aspects, and have speed characteristics fast, simple for structure.
The technical solution of the utility model is: a kind of nucleic acid extraction system and device comprises liquid distributing device, liquid-transfering device, sump assemblies and test tube group; Described liquid distributing device comprises the T-shaped gun rack and the eight road separatory volley of rifle fires; Described liquid-transfering device comprises liquid-transfering gun, Tip pipe, test-tube stand, water bath, shaking table, magnetic separating apparatus, eight road vacuum drainage systems; Described sump assemblies is made up of two reservoirs, conduit and negative pressure pumps; Described test tube group comprises eight test tubes, connect test tube under the eight road separatory volley of rifle fires, behind the reagent process separatory rifle separatory, become the equal capacity liquid in eight roads and flow to eight test tubes respectively, heat through water bath from eight pipes, after shaking table shakes up, test tube is placed under the liquid-transfering gun that the Tip pipe is housed, and liquid-transfering device links to each other with sump assemblies by conduit, move liquid with liquid-transfering gun after, magnetic separating apparatus will have effect biological tissue composition to be stayed in the test tube, and other liquid thereby are injected reservoir by the power of negative pressure pump through conduit.Described Tip pipe adopts the 3ml pipe, and test tube adopts the 10ml plastics tubing.Described magnetic separating apparatus magneticstrength is 3000～7000 Gausses.Described bottleneck separatory rifle range 0～25ml, each syringe needle separatory 0～3ml.
The beneficial effects of the utility model are: this device is a kind of extraction element fast and accurately, can solve the separatory problem, improve separatory rate and accuracy, avoid the error of manual operation, improve extraction efficiency, guarantee the quality of extract, have accuracy height, speed is fast, design is succinct advantage, be fit to promote the use of.
Description of drawings
Fig. 1 is the structural representation of the utility model nucleic acid extraction system and device.
Embodiment
As shown in Figure 1, the nucleic acid extraction system and device comprises liquid distributing device 1, liquid-transfering device 2, sump assemblies 3 and test tube group 4.Liquid distributing device 1 comprises the T-shaped gun rack and the eight road separatory volley of rifle fires; Liquid-transfering device 2 comprises liquid-transfering gun, Tip pipe and test-tube stand, water-bath, shaking table, magnetic separating apparatus, eight road vacuum drainage systems; Sump assemblies 3 comprises two reservoirs, conduit and negative pressure pump; Test tube group 4 comprises eight test tubes.
Mode of operation: connect test tube under the eight road separatory volley of rifle fires in the liquid distributing device, behind the reagent process separatory rifle separatory, become the equal liquid of eight road 1ml and flow to eight test tubes respectively from eight pipes, heat through water bath, shaking table shakes up, effective constituent has been adsorbed on the magnetic bead in the tissue juice at this moment, remaining all is water basically, be placed on the test-tube stand then, the Tip pipe is connected under the liquid-transfering gun, and test-tube stand and test tube together are put into the Tip pipe down, magnetic bead is arranged in the test tube, all there is magnet both sides before and after eight road test-tube stands, can adsorb magnetic bead, liquid-transfering device links to each other with sump assemblies by conduit, move liquid with liquid-transfering gun after, magnetic bead has effect biological tissue composition to be stayed in the test tube, and liquid thereby such as water are injected reservoir by the power of negative pressure pump through conduit.
The condition that the utility model is used is: 8 row, 6 row's schemes are adopted in 50-60 ℃ of test tube water-bath, Tip pipe and test-tube stand and base thereof, and wherein the Tip pipe adopts the 3ml pipe, and test tube adopts the 10ml plastic test tube.The magnetic separating apparatus magneticstrength is 3000-7000 Gauss under the test-tube stand.
The skimmer eight road volley of rifle fires can be divided equally the 8-24ml reagent that come out by skimmer, per pass 1-3ml, and error is smaller or equal to 5%.
This device is to overlap fast and accurately checkout gear, can solve the separatory problem, improves the separatory rate And accuracy, avoid the error of manual operation, improve extraction efficiency, guarantee the quality of extract. Therefore, Have the advantage that degree of accuracy height, speed are fast, design is succinct, be fit to promote the use of.
Claims (4)
1, a kind of nucleic acid extraction system and device is characterized in that comprising liquid distributing device, liquid-transfering device, sump assemblies and test tube group; Described liquid distributing device comprises the T-shaped gun rack and the eight road separatory volley of rifle fires; Described liquid-transfering device comprises liquid-transfering gun, Tip pipe, test-tube stand, water bath, shaking table, magnetic separating apparatus, eight road vacuum drainage systems; Described sump assemblies is made up of two reservoirs, conduit and negative pressure pumps; Described test tube group comprises eight test tubes, connect test tube under the eight road separatory volley of rifle fires, behind the reagent process separatory rifle separatory, become the equal capacity liquid in eight roads and flow to eight test tubes respectively, heat through water bath from eight pipes, after shaking table shakes up, test tube is placed under the liquid-transfering gun that the Tip pipe is housed, and liquid-transfering device links to each other with sump assemblies by conduit, move liquid with liquid-transfering gun after, magnetic separating apparatus will have effect biological tissue composition to be stayed in the test tube, and other liquid thereby are injected reservoir by the power of negative pressure pump through conduit.
2. nucleic acid extraction system and device according to claim 1 is characterized in that, described Tip pipe adopts the 3ml pipe, and test tube adopts the 10ml plastics tubing.
3. nucleic acid extraction system and device according to claim 1 is characterized in that, described magnetic separating apparatus magneticstrength is 3000～7000 Gausses.
4. nucleic acid extraction system and device according to claim 1 is characterized in that, described bottleneck separatory rifle range 0～25ml, each syringe needle separatory 0～3ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820057014 CN201201943Y (en) | 2008-04-08 | 2008-04-08 | Nucleic acid extracting system apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820057014 CN201201943Y (en) | 2008-04-08 | 2008-04-08 | Nucleic acid extracting system apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201201943Y true CN201201943Y (en) | 2009-03-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200820057014 Expired - Fee Related CN201201943Y (en) | 2008-04-08 | 2008-04-08 | Nucleic acid extracting system apparatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN201201943Y (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011150833A1 (en) * | 2010-05-31 | 2011-12-08 | 上海仁度生物科技有限公司 | Test tube, device comprising test tube and liquid treatment method by using same |
| CN103484363A (en) * | 2013-10-10 | 2014-01-01 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
| WO2015192329A1 (en) * | 2014-06-17 | 2015-12-23 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extractor waste liquid container, nucleic acid extractor, and control method |
| CN105316212A (en) * | 2014-07-28 | 2016-02-10 | 苏州康容生物医疗科技有限公司 | Magnetic bead method nucleic acid extraction treatment equipment |
| CN107841450A (en) * | 2017-12-21 | 2018-03-27 | 山西省农业科学院棉花研究所 | A kind of ethanol remove device and its application method for nucleic acid extraction experiment |
| CN108277148A (en) * | 2018-04-25 | 2018-07-13 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid distributing device |
| WO2019174524A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Nucleic acid extraction system |
| CN110869130A (en) * | 2017-07-19 | 2020-03-06 | 美国安进公司 | Magnetically assisted separation device and related methods |
| CN113308363A (en) * | 2021-06-02 | 2021-08-27 | 嘉兴科瑞迪医疗器械有限公司 | Sampling structure based on nucleic acid extraction appearance is gone on with separating liquid and imbibition in step |
-
2008
- 2008-04-08 CN CN 200820057014 patent/CN201201943Y/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011150833A1 (en) * | 2010-05-31 | 2011-12-08 | 上海仁度生物科技有限公司 | Test tube, device comprising test tube and liquid treatment method by using same |
| CN103484363A (en) * | 2013-10-10 | 2014-01-01 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
| CN103484363B (en) * | 2013-10-10 | 2015-01-07 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
| WO2015192329A1 (en) * | 2014-06-17 | 2015-12-23 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extractor waste liquid container, nucleic acid extractor, and control method |
| CN105940095A (en) * | 2014-06-17 | 2016-09-14 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extractor waste liquid container, nucleic acid extractor, and control method |
| CN105316212A (en) * | 2014-07-28 | 2016-02-10 | 苏州康容生物医疗科技有限公司 | Magnetic bead method nucleic acid extraction treatment equipment |
| US11433402B2 (en) | 2017-07-19 | 2022-09-06 | Amgen Inc. | Magnetic assisted separation apparatuses and related methods |
| CN110869130A (en) * | 2017-07-19 | 2020-03-06 | 美国安进公司 | Magnetically assisted separation device and related methods |
| CN107841450A (en) * | 2017-12-21 | 2018-03-27 | 山西省农业科学院棉花研究所 | A kind of ethanol remove device and its application method for nucleic acid extraction experiment |
| CN107841450B (en) * | 2017-12-21 | 2023-09-29 | 山西省农业科学院棉花研究所 | Ethanol removal device for nucleic acid extraction experiment and application method thereof |
| WO2019174524A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Nucleic acid extraction system |
| US11679382B2 (en) | 2018-03-13 | 2023-06-20 | Wuhan Edebio Technology Llc. | Nucleic acid extraction system |
| CN108277148A (en) * | 2018-04-25 | 2018-07-13 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid distributing device |
| CN108277148B (en) * | 2018-04-25 | 2024-05-28 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid separation device |
| CN113308363A (en) * | 2021-06-02 | 2021-08-27 | 嘉兴科瑞迪医疗器械有限公司 | Sampling structure based on nucleic acid extraction appearance is gone on with separating liquid and imbibition in step |
| CN113308363B (en) * | 2021-06-02 | 2023-11-14 | 嘉兴科瑞迪医疗器械有限公司 | Sampling structure based on synchronous liquid separation and liquid suction for nucleic acid extractor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090304 Termination date: 20100408 |