CN101503469A - Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film - Google Patents
Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film Download PDFInfo
- Publication number
- CN101503469A CN101503469A CNA2009101162924A CN200910116292A CN101503469A CN 101503469 A CN101503469 A CN 101503469A CN A2009101162924 A CNA2009101162924 A CN A2009101162924A CN 200910116292 A CN200910116292 A CN 200910116292A CN 101503469 A CN101503469 A CN 101503469A
- Authority
- CN
- China
- Prior art keywords
- soybean
- soybean protein
- isoflavones
- oligosaccharide
- stage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 72
- 244000068988 Glycine max Species 0.000 title claims abstract description 69
- 108010073771 Soybean Proteins Proteins 0.000 title claims abstract description 51
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 235000008696 isoflavones Nutrition 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 31
- 229920001542 oligosaccharide Polymers 0.000 title claims abstract description 27
- 150000002482 oligosaccharides Chemical class 0.000 title claims abstract description 27
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 title claims abstract description 7
- 229940001941 soy protein Drugs 0.000 title abstract description 5
- 108010046377 Whey Proteins Proteins 0.000 title description 5
- 102000007544 Whey Proteins Human genes 0.000 title description 5
- 239000005862 Whey Substances 0.000 title description 3
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 46
- 239000007788 liquid Substances 0.000 claims abstract description 40
- 239000012528 membrane Substances 0.000 claims abstract description 29
- 239000000047 product Substances 0.000 claims abstract description 24
- 239000011347 resin Substances 0.000 claims abstract description 22
- 229920005989 resin Polymers 0.000 claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000001694 spray drying Methods 0.000 claims abstract description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 13
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 12
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 238000010828 elution Methods 0.000 claims abstract description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 42
- 238000001179 sorption measurement Methods 0.000 claims description 32
- 150000002515 isoflavone derivatives Chemical class 0.000 claims description 28
- 239000010409 thin film Substances 0.000 claims description 17
- 239000007787 solid Substances 0.000 claims description 16
- 239000010408 film Substances 0.000 claims description 13
- 239000000284 extract Substances 0.000 claims description 11
- 239000003463 adsorbent Substances 0.000 claims description 10
- 238000002203 pretreatment Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 238000004382 potting Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 abstract description 7
- 239000012466 permeate Substances 0.000 abstract description 5
- 238000000605 extraction Methods 0.000 abstract description 3
- 235000003687 soy isoflavones Nutrition 0.000 abstract 3
- 230000002745 absorbent Effects 0.000 abstract 2
- 239000002250 absorbent Substances 0.000 abstract 2
- 239000012530 fluid Substances 0.000 abstract 2
- 238000004090 dissolution Methods 0.000 abstract 1
- 238000007781 pre-processing Methods 0.000 abstract 1
- 239000002351 wastewater Substances 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 5
- 239000011552 falling film Substances 0.000 description 4
- 235000013527 bean curd Nutrition 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 230000008020 evaporation Effects 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 235000021119 whey protein Nutrition 0.000 description 2
- 108010011756 Milk Proteins Proteins 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention relates to a method for extracting soy protein, oligosaccharide and isoflavone by a two-stage two-section membrane, which solves the problem of comprehensive extraction and utilization of soybean protein, oligosaccharide and isoflavone in soybean yellow serofluid. The method comprises four operating steps of: preprocessing: centrifuging the soybean yellow serofluid so as to remove bean dregs, suspended matter and the like and obtain supernatant fluid A; hyperfiltration: processing the supernatant fluid A by a two-stage two-section membrane system, realizing separation of soy protein with soy isoflavones and soy oligosaccharide and obtaining soy protein finished product with the content of more than 90 percent; absorption and reverse osmosis: processing permeate liquid by a macroporous absorbent resin column and a reverse osmosis membrane system, then carrying out concentration and spray drying and obtaining the soy oligosaccharide finished product with the content of more than 80 percent; and elution: carrying out dissolution and elution to soy isoflavone absorbed by the macroporous absorbent resin with 80 percent of methanol or alcohol, then carrying out concentration and spray drying and obtaining the soy isoflavone finished product with the content of more than 50 percent. The method has simple technique and convenient operation.
Description
Technical field
The present invention relates to utilize membrane separation technique from yellow plasm of soybean, to extract soybean protein, soybean oligosaccharide and soybean isoflavones functional factor product, belong to soybean deep processing and waste water reclaiming technical field.
Background technology
The a large amount of waste water that produce in the production process of bean product such as traditional bean curd, dried bean curd, the skin of soya-bean milk and soybean protein are called the bean curd whey wastewater, are commonly called as yellow seriflux.According to statistics, 1 ton of (t) soybean of every processing will be discharged 2-5 ton (t) yellow seriflux.Be rich in the functional factor extensively be familiar with such as soy-protein, soybean oligosaccharide, soybean isoflavones etc. in the yellow seriflux.Find after testing, the soybean isoflavones that contains in the yellow seriflux account for contained soybean isoflavones in the soy material 50%, stachyose 83%, raffinose 94%, proteinic 17%, have the selective separation recovery value.As far back as the seventies, Goldsmith has just carried out and utilizes membrane technique to reclaim the whey protein in the soya whey wastewater and the research of oligose, but disperse again because the scale of general Bean Products Factory is less, fund and technical force's deficiency, and recycling and problem of environmental pollution to functional factor are also paid little attention to, therefore, yellow plasm of soybean still is discharged as waste liquid so far, is not fully used.Therefore, seek the yellow seriflux treatment process of a kind of technical feasibility, industrial economy, environment protection standard, to promoting resource rational utilization, solving crisis of resource and environmental pollution has direct realistic meaning.
Treatment process to soybean processing waste water has anaerobic-aerobic biological treatment, evaporation concentration method, fermentation method and ultrafiltration-reverse osmosis-electroosmose process at present.Anaerobic-aerobic biological treatment treatment effect instability wherein; The evaporation concentration method energy consumption is big, and facility investment and working cost height were promoted in industrial being difficult to; The fermentation method products obtained therefrom is second-rate, mostly is small molecular protein sugar, and yields poorly; There is the shortcoming of operation of equipment complexity in ultrafiltration-reverse osmosis-electroosmose process.Membrane sepn be utilize a special manufacturing, have and select diactinic film, try hard to recommend outside moving down to the heterogeneity in liquid phase or the gas phase mixture separate, purification, spissated advanced machining technology.Membrane separation technique is easy and simple to handle with it, separation efficiency is high and the advantage such as low that consumes energy becomes the requisite technology of biologic separation and purification process.Adopt membrane separation technique to handle the Chinese patent that the existing publication number of soybean processing waste water is respectively CN4560047A and CN01336333A.Publication number provides a kind of method of utilizing Bean Products Factory's waste water to produce soybean isoflavones for the patent of invention of CN4560047A, but this technology does not relate to how extracting soybean protein and soybean oligosaccharide from yellow plasm of soybean.Publication number provides a kind of method of utilizing soybean processing waste water to produce soybean whey protein and soybean oligosaccharide for the patent of invention of CN01336333A, and how this patent of invention extracts isoflavones from yellow plasm of soybean if not relating to; And added in this extractive technique and contained Ca
2+Flocculation agent, concentration polarization and film very easily take place to be polluted, cause membrane flux to descend.
Summary of the invention
In order to solve the comprehensive extraction and application problem of soybean protein in the yellow plasm of soybean, oligose and isoflavones, the invention provides the method for soybean protein, oligose and isoflavones in the employing two-stage two-segment film extraction yellow plasm of soybean that a kind of technology is simple, easy to operate, added value is high.
The technical solution that realizes above-mentioned purpose is as follows:
The method that two-stage two-segment film extracts soybean protein, oligose and isoflavones in the yellow plasm of soybean comprises following operation steps:
(1), pre-treatment:
The yellow plasm of soybean that produces in the bean product production process directly through the centrifugal pre-treatment of whizzer, to remove bean dregs, suspended substance wherein, is obtained supernatant A;
(2), ultrafiltration:
Is 50000-100000 daltonian one-level one section ultra-filtration membrane ultrafiltration by molecular weight cut-off with supernatant A under temperature 20-50 ℃, pressure 0.5-1.5Mpa, obtains soybean protein concentrated solution P1A, and obtains ultrafiltration and see through liquid B
0Again ultrafiltration is seen through liquid B
0By molecular weight cut-off is two sections ultra-filtration membrane ultrafiltration of 3000 daltonian one-levels, obtains soybean protein concentrated solution P1
B, and obtain ultrafiltration through liquid B; Merge soybean protein concentrated solution P1
AWith soybean protein concentrated solution P1
BPromptly obtain soybean protein concentrated solution P1, soybean protein concentrated solution P1 is concentrated into solid content through thin-film evaporator reaches 25%-30%, enter spray-drying tower again and carry out spraying drying, obtain the soybean protein finished product of content more than 90%;
(3), absorption and reverse osmosis:
Ultrafiltration is seen through the adsorption column of liquid B through macroporous adsorbent resin; Soybean isoflavones is by the absorption of the adsorption column of macroporous adsorbent resin, and soybean oligosaccharide flows out from the adsorption column of macroporous adsorbent resin, collects effluent liquid C; Effluent liquid C by reverse osmosis membrane system, is obtained soybean oligosaccharide concentrated solution P2; Soybean oligosaccharide concentrated solution P2 is concentrated into solid content through thin-film evaporator reaches 25%-30%, enter spray-drying tower again and carry out spraying drying, obtain the soybean oligosaccharide finished product of content more than 80%;
(4), wash-out:
Earlier with the water elution liquid of deionized water wash-out adsorption column to the effluent liquid solid substance, the back with the methyl alcohol more than 80% or ethanol under the flow velocity of 1-3BV/h the wash-out adsorption column to the alcohol eluen that flows out isoflavone-containing; Alcohol eluen is concentrated into solid content with thin-film evaporator reaches 25%-30%, enter spray-drying tower again and carry out spraying drying, obtain the soybean isoflavones finished product of content more than 50%.
Whizzer in the step (1) is a disk centrifugal separator, and centrifuging temperature is 10-60 ℃, and working flow is 1-2L/min.
The processing condition of one section ultra-filtration membrane ultrafiltration of one-level or two sections ultra-filtration membrane ultrafiltration of one-level are in the step (2): temperature 20-50 ℃, pressure 0.5-1.5Mpa.
Reverse osmosis pressure described in the step (3) is 0.2-2.0Mpa.
The potting resin of adsorption column described in the step (3) is D101 or AB-8 nonpolar macroporous adsorption resin.
The inlet temperature 125-130 of described spray-drying tower ℃, air outlet temperature 80-85 ℃.
The working conditions of described thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃.Thin film evaporation reclaims the methyl alcohol or the ethanol that obtain and can be used as the eluent recycling in the described step (4).
Useful technique effect of the present invention is embodied in the following aspects:
1, this technology has overcome problems of the prior art and deficiency, has the advantages that technology is simple, flow process is short, cost is low, is fit to large, medium and small different scales bean product processing enterprise.
2, the present invention has realized the recycling of yellow plasm of soybean, has not only obtained the three kind biologically active substances useful to HUMAN HEALTH, and has reduced environmental pollution, has important economic benefit, social benefit and environmental benefit.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described by embodiment.
Embodiment 1:
Referring to Fig. 1, the method that two-stage two-segment film extracts soybean protein, oligose and isoflavones in the yellow plasm of soybean comprises following operation steps:
Step 1: pre-treatment
The yellow plasm of soybean that produces in the bean product production process is collected into wastewater trough, entering rotating speed through the waste water force (forcing) pump is that 10000r/min disk centrifugal separator above, that flow is 2L/min carries out centrifugal pre-treatment, centrifuging temperature is 10-60 ℃, to remove wherein bean dregs, particle, suspended substance etc., obtain supernatant A;
Step 2: ultrafiltration
Is 50000-100000 daltonian one-level one section ultrafiltration membrane system by molecular weight cut-off successively with the supernatant A that obtains after the pre-treatment under temperature 20-50 ℃, pressure 0.5-1.5Mpa condition, obtains soybean protein concentrated solution P1
A, and obtain ultrafiltration membrane permeate liquid B
0Again with ultrafiltration membrane permeate liquid B
0By molecular weight cut-off is two sections ultrafiltration membrane system of 3000 daltonian one-levels, and the realization soybean protein separates with soybean isoflavones, soybean oligosaccharide, obtains soybean protein concentrated solution P1
B, and obtain ultrafiltration through liquid B; Merge soybean protein concentrated solution P1
AWith soybean protein concentrated solution P1
BPromptly obtain soybean protein concentrated solution P1, soybean protein concentrated solution P1 is concentrated into solid content through falling-film evaporator reaches 25%-30%, the working conditions of thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃; Enter spray-drying tower again, inlet temperature 125-130 ℃, air outlet temperature 80-85 ℃, to obtain the soybean protein part finished product of content more than 90%.
Step 3: absorption and reverse osmosis
Resulting ultrafiltration in the step 2 is seen through liquid B from bottom to top through an adsorption column that is assembled with the AB-8 macroporous adsorbent resin; The adsorption column group is made up of three adsorption columns, and ultrafiltration sees through liquid B in succession continuously in an orderly manner by three adsorption columns; Be filled with according to the AB-8 macroporous adsorbent resin that sees through liquid weight 10% in every three adsorption columns; Soybean isoflavones is by resin absorption, and soybean oligosaccharide flows out from resin column, collects effluent liquid C; By reverse osmosis membrane system, reverse osmosis pressure is 0.2-2.0Mpa, obtains soybean oligosaccharide concentrated solution P2 with effluent liquid C; Soybean oligosaccharide concentrated solution P2 is concentrated into solid content through falling-film evaporator reaches 25%-30%, the working conditions of thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃; Enter spray-drying tower again, inlet temperature 125-130 ℃, air outlet temperature 80-85 ℃, to obtain the soybean oligosaccharide finished product of content more than 80%.
Adsorption column potting resin or employing D101 nonpolar macroporous adsorption resin.
Step 4: wash-out
After adsorption operations is finished, use deionized water wash-out adsorption column to effluent liquid solid content＜0.5% (refractometer analysis) earlier, the back obtains water elution liquid and alcohol eluen with the wash-out adsorption column under the flow velocity of 1-3BV/h of the methyl alcohol more than 80% to effluent liquid isoflavone content＜0.001% (spectrophotometric analysis); Alcohol eluen concentrates with thin-film evaporator, and the working conditions of thin-film evaporator: vacuum tightness 0.08-0.09Mpa, temperature 50-60 ℃, and be concentrated into solid content and reach 25%-30%; Enter spray-drying tower again, inlet temperature 125-130 ℃, air outlet temperature 80-85 ℃, to obtain the soybean isoflavones finished product of content more than 50%.
Embodiment 2
Step 1: pre-treatment
Getting the 1000L yellow plasm of soybean, to add rotating speed be that 20000r/min disk centrifugal separator above, that flow is 2L/min carries out centrifugal pre-treatment, and centrifuging temperature is 10-60 ℃, to remove wherein bean dregs, particle, suspended substance etc., obtains the 974L supernatant A;
Step 2: ultrafiltration
Is 50000-100000 daltonian one-level one section ultrafiltration membrane system by molecular weight cut-off successively with the supernatant A that obtains after the pre-treatment under 20-50 ℃, 0.5-1.5Mpa working pressure, obtains soybean protein concentrated solution P1
A, and obtain ultrafiltration membrane permeate liquid B
0Again with ultrafiltration membrane permeate liquid B
0By molecular weight cut-off is two sections ultrafiltration membrane system of 3000 daltonian one-levels, and the realization soybean protein separates with soybean isoflavones, soybean oligosaccharide, obtains soybean protein concentrated solution P1
B, and obtain ultrafiltration through liquid B; Merge soybean protein concentrated solution P1
AWith soybean protein concentrated solution P1
BPromptly obtain soybean protein concentrated solution P1, soybean protein concentrated solution P1 is concentrated into solid content through falling-film evaporator reaches 25%-30%, the working conditions of thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃; Enter spray-drying tower again, inlet temperature 125-130 ℃, air outlet temperature 80-85 ℃, to obtain soybean protein part finished product 995g of content 91.2%.
Step 3: absorption and reverse osmosis
Resulting ultrafiltration in the step 2 is seen through liquid B from bottom to top through an adsorption column that is assembled with the AB-8 macroporous adsorbent resin; The adsorption column group is made up of three adsorption columns, and ultrafiltration sees through liquid B in succession continuously in an orderly manner by three adsorption columns; Be filled with according to the AB-8 macroporous adsorbent resin that sees through liquid weight 10% in every three adsorption columns; Soybean isoflavones is by resin absorption, and soybean oligosaccharide flows out from resin column, collects effluent liquid C; Effluent liquid C by reverse osmosis membrane system, is obtained soybean oligosaccharide concentrated solution P2; Soybean oligosaccharide concentrated solution P2 is concentrated into solid content through falling-film evaporator reaches 25%-30%, the working conditions of thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃; Enter spray-drying tower again, inlet temperature 125-130 ℃, air outlet temperature 80-85 ℃, to obtain the soybean oligosaccharide finished product 12450g of content 80.6%.
Step 4: wash-out
After adsorption operations is finished, use deionized water wash-out adsorption column to effluent liquid solid content＜0.5% (refractometer analysis) earlier, the back obtains water elution liquid and alcohol eluen with the wash-out adsorption column under the flow velocity of 1-3BV/h of the ethanol more than 80% to effluent liquid isoflavone content＜0.001% (spectrophotometric analysis); Alcohol eluen concentrates with thin-film evaporator, the working conditions of thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃; And be concentrated into solid content and reach 25%-30%, enter spray-drying tower again, 125 ℃ of inlet temperature, 80 ℃ of air outlet temperatures are to obtain the soybean isoflavones finished product 64g of content 52.1%.
Claims (7)
1, two-stage two-segment film extracts the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean, it is characterized in that comprising following operation steps:
(1), pre-treatment:
The yellow plasm of soybean that produces in the bean product production process directly through the centrifugal pre-treatment of whizzer, to remove bean dregs, suspended substance wherein, is obtained supernatant A;
(2), ultrafiltration:
Is 50000-100000 daltonian one-level one section ultra-filtration membrane ultrafiltration by molecular weight cut-off with supernatant A under temperature 20-50 ℃, pressure 0.5-1.5Mpa, obtains soybean protein concentrated solution P1
A, and obtain ultrafiltration through liquid B
0Again ultrafiltration is seen through liquid B
0By molecular weight cut-off is two sections ultra-filtration membrane ultrafiltration of 3000 daltonian one-levels, obtains soybean protein concentrated solution P1
B, and obtain ultrafiltration through liquid B; Merge soybean protein concentrated solution P1
AWith soybean protein concentrated solution P1
BPromptly obtain soybean protein concentrated solution P1, soybean protein concentrated solution P1 is concentrated into solid content through thin-film evaporator reaches 25%-30%, enter spray-drying tower again and carry out spraying drying, obtain the soybean protein finished product of content more than 90%;
(3), absorption and reverse osmosis:
Ultrafiltration is seen through the adsorption column of liquid B through macroporous adsorbent resin; Soybean isoflavones is by the absorption of the adsorption column of macroporous adsorbent resin, and soybean oligosaccharide flows out from the adsorption column of macroporous adsorbent resin, collects effluent liquid C; Effluent liquid C by reverse osmosis membrane system, is obtained soybean oligosaccharide concentrated solution P2; Soybean oligosaccharide concentrated solution P2 is concentrated into solid content through thin-film evaporator reaches 25%-30%, enter spray-drying tower again and carry out spraying drying, obtain the soybean oligosaccharide finished product of content more than 80%;
(4), wash-out:
Earlier with the water elution liquid of deionized water wash-out adsorption column to the effluent liquid solid substance, the back with the methyl alcohol more than 80% or ethanol under the flow velocity of 1-3BV/h the wash-out adsorption column to the alcohol eluen that flows out isoflavone-containing; Alcohol eluen is concentrated into solid content with thin-film evaporator reaches 25%-30%, enter spray-drying tower again and carry out spraying drying, obtain the soybean isoflavones finished product of content more than 50%.
2, extract the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean according to the described two-stage two-segment film of claim 1, it is characterized in that: the whizzer in the step (1) is a disk centrifugal separator, and centrifuging temperature is 10-60 ℃, and working flow is 1-2L/min.
3, extract the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean according to the described two-stage two-segment film of claim 1, it is characterized in that: the processing condition of one section ultra-filtration membrane ultrafiltration of one-level or two sections ultra-filtration membrane ultrafiltration of one-level are in the step (2): temperature 20-50 ℃, pressure 0.5-1.5Mpa.
4, extract the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean according to the described two-stage two-segment film of claim 1, it is characterized in that: the reverse osmosis pressure described in the step (3) is 0.2-2.0Mpa.
5, extract the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean according to the described two-stage two-segment film of claim 1, it is characterized in that: the potting resin of adsorption column described in the step (3) is D101 or AB-8 nonpolar macroporous adsorption resin.
6, extract the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean according to the described two-stage two-segment film of claim 1, it is characterized in that: the inlet temperature 125-130 of described spray-drying tower ℃, air outlet temperature 80-85 ℃.
7, extract the method for soybean protein, oligose and isoflavones in the yellow plasm of soybean according to the described two-stage two-segment film of claim 1, it is characterized in that: the working conditions of described thin-film evaporator: vacuum tightness 0.08-0.09MPa, temperature 50-60 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101162924A CN101503469B (en) | 2009-03-06 | 2009-03-06 | Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101162924A CN101503469B (en) | 2009-03-06 | 2009-03-06 | Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101503469A true CN101503469A (en) | 2009-08-12 |
| CN101503469B CN101503469B (en) | 2011-10-26 |
Family
ID=40975861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101162924A Active CN101503469B (en) | 2009-03-06 | 2009-03-06 | Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101503469B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2301367A1 (en) * | 2009-09-28 | 2011-03-30 | WhiteWave Services, Inc. | Soy beverage substantially free of isoflavones and method of production |
| CN102827687A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Extraction method of soybean oligosaccharides |
| CN103265614A (en) * | 2013-05-28 | 2013-08-28 | 宜宾学院 | Method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by one-step process |
| CN106282258A (en) * | 2016-09-24 | 2017-01-04 | 合肥信达膜科技有限公司 | A kind of soybean isoflavone isolation technics |
| CN106923197A (en) * | 2015-12-30 | 2017-07-07 | 丰益(上海)生物技术研发中心有限公司 | A kind of soybean water extract of low anti-nutrition component and preparation method thereof |
| CN108308368A (en) * | 2018-03-21 | 2018-07-24 | 山东禹王生态食业有限公司 | A kind of soybean protein bean clear water processing system and processing method |
| CN108587798A (en) * | 2018-04-09 | 2018-09-28 | 吕慧琴 | A kind of natural handguard dish washing detergent and preparation method thereof |
| CN110003289A (en) * | 2019-05-08 | 2019-07-12 | 江苏博晟康生物科技有限公司 | A method of the yellow serofluid improving biological transformation ratio prepares soyabean oligosaccharides |
| CN111944010A (en) * | 2020-08-20 | 2020-11-17 | 运城学院 | Equipment and method for extracting soybean protein, isoflavone and oligosaccharide from bean product wastewater |
| CN112456713A (en) * | 2020-11-24 | 2021-03-09 | 广东闻扬环境科技有限公司 | Process and equipment for recovering and treating protein in bean product wastewater |
| CN113355372A (en) * | 2021-06-10 | 2021-09-07 | 苏州金记食品有限公司 | Process for purifying soybean whey and extracting soybean isoflavone aglycone |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116657A (en) * | 1995-04-24 | 1996-02-14 | 国内贸易部西安油脂科学研究设计院 | Process for producing soya oligose by membrane separation process |
| CN1133591C (en) * | 2001-09-25 | 2004-01-07 | 清华同方股份有限公司 | Membrane separation process of treating soybean processing waste water |
| CN1537865A (en) * | 2003-10-23 | 2004-10-20 | 姜浩奎 | Method of extracting lactoalbumin, nucleic acid oligosaccharide, isoflavone, saponin from yellow waste water generated by producing soybean |
| CN100348518C (en) * | 2005-08-05 | 2007-11-14 | 武汉湖工大膜技术开发有限公司 | Treatment method of soybean lactalbumin waste water |
-
2009
- 2009-03-06 CN CN2009101162924A patent/CN101503469B/en active Active
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2301367A1 (en) * | 2009-09-28 | 2011-03-30 | WhiteWave Services, Inc. | Soy beverage substantially free of isoflavones and method of production |
| US8597712B2 (en) | 2009-09-28 | 2013-12-03 | Whitewave Services, Inc. | Soy beverage substantially free of isoflavones and method of production |
| CN102827687A (en) * | 2012-09-17 | 2012-12-19 | 东北农业大学 | Extraction method of soybean oligosaccharides |
| CN103265614A (en) * | 2013-05-28 | 2013-08-28 | 宜宾学院 | Method for extracting soy protein, oligosaccharide and isoflavone from soybean wastewater by one-step process |
| CN106923197A (en) * | 2015-12-30 | 2017-07-07 | 丰益(上海)生物技术研发中心有限公司 | A kind of soybean water extract of low anti-nutrition component and preparation method thereof |
| CN106282258A (en) * | 2016-09-24 | 2017-01-04 | 合肥信达膜科技有限公司 | A kind of soybean isoflavone isolation technics |
| CN108308368A (en) * | 2018-03-21 | 2018-07-24 | 山东禹王生态食业有限公司 | A kind of soybean protein bean clear water processing system and processing method |
| CN108308368B (en) * | 2018-03-21 | 2021-05-28 | 山东禹王生态食业有限公司 | Soybean protein clear water treatment system and method |
| CN108587798A (en) * | 2018-04-09 | 2018-09-28 | 吕慧琴 | A kind of natural handguard dish washing detergent and preparation method thereof |
| CN110003289A (en) * | 2019-05-08 | 2019-07-12 | 江苏博晟康生物科技有限公司 | A method of the yellow serofluid improving biological transformation ratio prepares soyabean oligosaccharides |
| CN111944010A (en) * | 2020-08-20 | 2020-11-17 | 运城学院 | Equipment and method for extracting soybean protein, isoflavone and oligosaccharide from bean product wastewater |
| CN111944010B (en) * | 2020-08-20 | 2023-05-09 | 运城学院 | Extraction equipment and method for soybean protein, isoflavone and oligosaccharide in bean product wastewater |
| CN112456713A (en) * | 2020-11-24 | 2021-03-09 | 广东闻扬环境科技有限公司 | Process and equipment for recovering and treating protein in bean product wastewater |
| CN113355372A (en) * | 2021-06-10 | 2021-09-07 | 苏州金记食品有限公司 | Process for purifying soybean whey and extracting soybean isoflavone aglycone |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101503469B (en) | 2011-10-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101503469B (en) | Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film | |
| CN101870722B (en) | Process for concentrating protein in soy protein wastewater by two-stage foam separation method | |
| CN103570664B (en) | Method of extracting procyanidine from huckleberries | |
| CN102178003A (en) | Process for extracting tea polyphenol | |
| CN102070688A (en) | Method for enriching and purifying icariin in epimedium herb | |
| CN102093748A (en) | Method for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes | |
| CN102633900A (en) | Method for extracting and purifying polysaccharide from Cordyceps militaris | |
| CN104130310A (en) | Separation and purification method for glutathione | |
| CN102838576B (en) | Process for concentrating soy isoflavone in soy protein waste water by foam separation method | |
| CN201250196Y (en) | Integral device for microwave extracting, hyperfiltration separating and nanofiltration condensing of astragalus polyose | |
| CN203916141U (en) | A kind of gynostemma pentaphylla flavones and polysaccharide extract the device of purifying continuously | |
| CN102274386A (en) | Method for extracting total isoflavones from Allium mongolicum Regel | |
| CN102204950A (en) | Method for extracting flavonoid from liquorice waste residue | |
| CN102319306A (en) | A kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides | |
| CN105193880A (en) | Extraction method for actinidia arguta flavones | |
| CN101759735A (en) | Method for preparing arctin from burdock | |
| CN102558251A (en) | Production method of ultrafiltration membrane for extracting tannin from banana stems stoste | |
| CN102488218A (en) | Method for extracting and preparing high-content peanut coat polyphenol from peanut coat | |
| CN106432533A (en) | Purification method of astragalus membranaceus polysaccharide | |
| CN103965276B (en) | The method of fast separating and purifying monomeric compound from Lindley Eupatorium Herb | |
| CN202011849U (en) | Equipment for extracting and concentrating polysaccharide from medicinal materials | |
| CN102993143B (en) | Method for rapidly separating alkannic acid monomer from salviae miltiorrhizae | |
| CN201710997U (en) | Energy-saving plant active ingredient extracting and concentrating device | |
| CN102002029B (en) | Method for separating and extracting isoflavone by simulated moving bed adsorption | |
| CN105477029A (en) | Preparation method of evening primrose seed flavone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 245400 Longwan Industrial Zone, Xiuning Town, Anhui, Huangshan City, China, five Patentee after: HUANGSHAN JIALONG GREEN FOOD CO.,LTD. Address before: 245400 Longwan Industrial Zone, Xiuning Town, Anhui, Huangshan City, China, five Patentee before: HUANGSHAN TOURISM GROUP JIALONG GREEN FOOD CO.,LTD. |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for extracting soy protein, oligosaccharide and isoflavone from soybean whey by two-stage two-segment film Effective date of registration: 20180209 Granted publication date: 20111026 Pledgee: Xiuning Anhui rural commercial bank Limited by Share Ltd. Pledgor: HUANGSHAN JIALONG GREEN FOOD CO.,LTD. Registration number: 2018340000049 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210204 Granted publication date: 20111026 Pledgee: Xiuning Anhui rural commercial bank Limited by Share Ltd. Pledgor: HUANGSHAN JIALONG GREEN FOOD Co.,Ltd. Registration number: 2018340000049 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Extraction of soybean protein, oligosaccharides and isoflavones from soybean yellow pulp by two stage two stage membrane Effective date of registration: 20210207 Granted publication date: 20111026 Pledgee: Xiuning Anhui rural commercial bank Limited by Share Ltd. Pledgor: HUANGSHAN JIALONG GREEN FOOD Co.,Ltd. Registration number: Y2021980001094 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20111026 Pledgee: Xiuning Anhui rural commercial bank Limited by Share Ltd. Pledgor: HUANGSHAN JIALONG GREEN FOOD CO.,LTD. Registration number: Y2021980001094 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for Extraction of Soybean Protein, Oligosaccharides, and Isoflavones from Soybean Yellow Milk Water by Secondary and Secondary Membrane Extraction Granted publication date: 20111026 Pledgee: Xiuning Anhui rural commercial bank Limited by Share Ltd. Pledgor: HUANGSHAN JIALONG GREEN FOOD CO.,LTD. Registration number: Y2024980003998 |