CN106432533A - Purification method of astragalus membranaceus polysaccharide - Google Patents
Purification method of astragalus membranaceus polysaccharide Download PDFInfo
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- CN106432533A CN106432533A CN201611132103.9A CN201611132103A CN106432533A CN 106432533 A CN106432533 A CN 106432533A CN 201611132103 A CN201611132103 A CN 201611132103A CN 106432533 A CN106432533 A CN 106432533A
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- astragalus polysaccharidess
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention relates to a purification method of an astragalus membranaceus polysaccharide, and belongs to the technical field of polysaccharide purification. A macroporous adsorption resin chromatography decoloring technology is combined with an ion exchange resin desalting technology to purify the astragalus membranaceus polysaccharide extracted from astragalus membranaceus, and the high-purity and high-content astragalus membranaceus polysaccharide is thus prepared. Compared with the traditional process, the purification method has the advantages that the purification process is simple and easy to implement, the production efficiency is high, the product yield is high, the product purity is high, the process is environmentally friendly and the production cost is low.
Description
Technical field
A kind of a kind of the present invention relates to purification process of polysaccharide, it is more particularly related to purification of astragalus polysaccharidess
Method, belongs to polysaccharide purification technical field.
Background technology
In Chinese herbal medicine, contained polysaccharide is the natural materials that a class has physiologically active, and major part is distributed in multiple Chinese herbal medicine
In, it is one of principle active component of Chinese herbal medicine.And the Radix Astragali is the dry root of leguminous plant Radix Astagali or Radix Astragali, it is weight
The QI invigorating Chinese medicine wanted.Astragalus polysaccharidess are most important natural constituents in the Radix Astragali, have promotion immunologic function, improve huge biting carefully
Cytoactive, two-ways regulation blood glucose etc. act on.Active anticancer, reductionization are remarkably improved using astragalus polysaccharidess and ginseng polysaccharide's compatibility
Treat side effect.Meanwhile, research finds biological activity and molecular weight, dissolubility, viscosity, primary structure and the senior knot of astragalus polysaccharidess
Structure is relevant.Therefore, during Polyose extraction, should be according to the difference of its effective ingredient come design technology route.
Extraction process by water is mainly taken in the at present extraction of astragalus polysaccharidess, and the shortcoming of this technique is that the yield of polysaccharide crude is relatively low,
Extract obtained polyoses content is not high, contains excessive pigment and inorganic salt in product, in addition extraction cost very high so that the Radix Astragali
The exploitation further of polysaccharide are restricted.
Content of the invention
Invention aims to solve the problem that the problem of existing astragalus polysaccharidess purification, provides a kind of purification process of astragalus polysaccharidess, should
Method production efficiency is high, product yield is high, product purity is high, process environments are friendly, low production cost.
In order to realize foregoing invention purpose, its specific technical scheme is as follows：
A kind of purification process of astragalus polysaccharidess it is characterised in that：Comprise the following steps that：
A, macroporous adsorbent resin is washed till effluent with 95% ethanol does not produce white opacity in water；The resin processing is turned
Move in chromatographic column；It is washed with deionized water the ethanol in macroporous resin in net chromatographic column, standby；
B, made with the flow velocity of 20ml/min astragalus polysaccharidess extracting solution 500ml pass through resin chromatography post；It is washed with deionized water chromatography
The astragalus polysaccharidess extracting solution of residual in post；Collect effluent and be used for next step operation；
C, wash away the various pigment regeneration resin columns of resin absorption with the ethanol of 75-80%；After regeneration, macroporous resin chromatographic column can weigh
Multiple use；
D, accurate measuring strong acid ion exchange resin, strong alkali ion exchange resin, fill in two chromatographic columns respectively；By step B
The astragalus polysaccharidess extracting solution obtaining passes through above-mentioned two chromatographic columns respectively with the flow velocity of 10ml/min；Collect effluent, concentration obtains final product
Purifying high-purity astragalus polysaccharidess.
In step, described macroporous adsorbent resin is 50ml to the present invention.
In step, described 95% alcohol smell is 1000ml to the present invention.
In step C, the ethanol of described 75-80% is 200ml to the present invention.
In step D, described strong acid ion exchange resin and strong alkali ion exchange resin are all 50ml to the present invention.
The Advantageous Effects that the present invention brings：
The present invention adopts macroporous adsorption resin chromatography decolouring technology coupled ion exchanger resin desalting technology, to the Radix Astragali being extracted
Astragalus polysaccharidess carry out purification, are prepared for a kind of higher astragalus polysaccharidess of content purity.There is compared with traditional handicraft purifying process
Simple, production efficiency is high, product yield is high, product purity is high, process environments are friendly, the advantages of low production cost.
Specific embodiment
Embodiment 1
A kind of purification process of astragalus polysaccharidess, comprises the following steps that：
A, macroporous adsorbent resin is washed till effluent with 95% ethanol does not produce white opacity in water；The resin processing is turned
Move in chromatographic column；It is washed with deionized water the ethanol in macroporous resin in net chromatographic column, standby；
B, made with the flow velocity of 20ml/min astragalus polysaccharidess extracting solution 500ml pass through resin chromatography post；It is washed with deionized water chromatography
The astragalus polysaccharidess extracting solution of residual in post；Collect effluent and be used for next step operation；
C, wash away the various pigment regeneration resin columns of resin absorption with the ethanol of 75-80%；After regeneration, macroporous resin chromatographic column can weigh
Multiple use；
D, accurate measuring strong acid ion exchange resin, strong alkali ion exchange resin, fill in two chromatographic columns respectively；By step B
The astragalus polysaccharidess extracting solution obtaining passes through above-mentioned two chromatographic columns respectively with the flow velocity of 10ml/min；Collect effluent, concentration obtains final product
Purifying high-purity astragalus polysaccharidess.
Embodiment 2
On the basis of embodiment 1：
Preferably, in step, described macroporous adsorbent resin is 50ml.
Preferably, in step, described 95% alcohol smell is 1000ml.
Preferably, in step D, described strong acid ion exchange resin and strong alkali ion exchange resin are all 50ml.
Claims (4)
1. a kind of purification process of astragalus polysaccharidess it is characterised in that：Comprise the following steps that：
A, macroporous adsorbent resin is washed till effluent with 95% ethanol does not produce white opacity in water；The resin processing is turned
Move in chromatographic column；It is washed with deionized water the ethanol in macroporous resin in net chromatographic column, standby；
B, made with the flow velocity of 20ml/min astragalus polysaccharidess extracting solution 500ml pass through resin chromatography post；It is washed with deionized water chromatography
The astragalus polysaccharidess extracting solution of residual in post；Collect effluent and be used for next step operation；
C, wash away the various pigment regeneration resin columns of resin absorption with the ethanol of 75-80%；After regeneration, macroporous resin chromatographic column can weigh
Multiple use；
D, accurate measuring strong acid ion exchange resin, strong alkali ion exchange resin, fill in two chromatographic columns respectively；By step B
The astragalus polysaccharidess extracting solution obtaining passes through above-mentioned two chromatographic columns respectively with the flow velocity of 10ml/min；Collect effluent, concentration obtains final product
Purifying high-purity astragalus polysaccharidess.
2. a kind of astragalus polysaccharidess according to claim 1 purification process it is characterised in that：In step, described macropore
Adsorbent resin is 50ml.
3. a kind of astragalus polysaccharidess according to claim 1 purification process it is characterised in that：In step, described 95%
Alcohol smell be 1000ml.
4. a kind of astragalus polysaccharidess according to claim 1 purification process it is characterised in that：In step D, described strong acid from
Sub-exchange resin and strong alkali ion exchange resin are all 50ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611132103.9A CN106432533A (en) | 2016-12-09 | 2016-12-09 | Purification method of astragalus membranaceus polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611132103.9A CN106432533A (en) | 2016-12-09 | 2016-12-09 | Purification method of astragalus membranaceus polysaccharide |
Publications (1)
| Publication Number | Publication Date |
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| CN106432533A true CN106432533A (en) | 2017-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611132103.9A Pending CN106432533A (en) | 2016-12-09 | 2016-12-09 | Purification method of astragalus membranaceus polysaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112263522A (en) * | 2020-11-05 | 2021-01-26 | 广州市中通生化制品有限公司 | Composition for relieving and repairing skin and application thereof |
| CN117700575A (en) * | 2023-12-06 | 2024-03-15 | 佛山科学技术学院 | Carboxymethyl astragalus polysaccharide with high water solubility and oxidation resistance and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197078A (en) * | 1997-04-21 | 1998-10-28 | 南京金陵制药(集团)有限公司 | Novel production tech. of holoside |
| CN101904863A (en) * | 2009-06-04 | 2010-12-08 | 广州泽力医药科技有限公司 | Preparation process of medicinal composition compounded from astragaloside and astragalus polyose |
-
2016
- 2016-12-09 CN CN201611132103.9A patent/CN106432533A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197078A (en) * | 1997-04-21 | 1998-10-28 | 南京金陵制药(集团)有限公司 | Novel production tech. of holoside |
| CN101904863A (en) * | 2009-06-04 | 2010-12-08 | 广州泽力医药科技有限公司 | Preparation process of medicinal composition compounded from astragaloside and astragalus polyose |
Non-Patent Citations (1)
| Title |
|---|
| 罗永明主编: "《中药化学成分提取分离技术与方法（第1版）》", 31 January 2016, 上海科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112263522A (en) * | 2020-11-05 | 2021-01-26 | 广州市中通生化制品有限公司 | Composition for relieving and repairing skin and application thereof |
| CN112263522B (en) * | 2020-11-05 | 2023-05-19 | 广州市中通生化制品有限公司 | Composition for relieving and repairing skin and application thereof |
| CN117700575A (en) * | 2023-12-06 | 2024-03-15 | 佛山科学技术学院 | Carboxymethyl astragalus polysaccharide with high water solubility and oxidation resistance and preparation method thereof |
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Application publication date: 20170222 |