CN102319306A - A kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides - Google Patents
A kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides Download PDFInfo
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- CN102319306A CN102319306A CN201110264830A CN201110264830A CN102319306A CN 102319306 A CN102319306 A CN 102319306A CN 201110264830 A CN201110264830 A CN 201110264830A CN 201110264830 A CN201110264830 A CN 201110264830A CN 102319306 A CN102319306 A CN 102319306A
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- fructus sorbi
- total cyanogen
- cyanogen glycosides
- sorbi tianschanicae
- tianschanicae
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Abstract
The invention discloses a kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides.Method is: dry Fructus Sorbi Tianschanicae leaf is pulverized, added doubly 50% alcohol solution dipping of 10-18, squeezing is also filtered, and filtrating adds active carbon insulation decolouring; Filter, reclaim ethanol, concentrated solution is centrifugal, and supernatant adds appropriate amount of deionized water; Through the film separation system ultrafiltration, macroporous resin column absorption on the permeate, 20-35% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides.Technology of the present invention is simple to operation, energy consumption is low, and the product property of acquisition is stable, is easy to realize the industrialization amplification.
Description
Technical field
The present invention relates to a kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides, especially relate to a kind of method that adopts membrance separation and resin column adsorption technology from Fructus Sorbi Tianschanicae, to extract total cyanogen glycosides.
Background technology
Fructus Sorbi Tianschanicae is a rosaceous plant, among the peoplely often is used as medicine with fruit, branch and leaf and peel of stem.Fructus Sorbi Tianschanicae has the effect that clearing away lung-heat to relieve cough, spleen reinforcing promote the production of body fluid.Contain flavone compound, anthocyanidin in the Fructus Sorbi Tianschanicae, give birth to cyanogen glycosides etc.Pharmacological evaluation shows, multiple efficacies such as the Fructus Sorbi Tianschanicae branch and leaf have cough-relieving concurrently, relieving asthma, heart tonifying, and toxicity is very low.(Chinese TCM basis medical journal, 2001,7 (1): 45-47) discover that effective ingredient is mainly flavonoid and cyanogen glycosides compound in the Fructus Sorbi Tianschanicae such as Chen Jian.
Bibliographical information is arranged, and the cyanogen glycosides in the Fructus Sorbi Tianschanicae leaf is higher than content in the peel of stem, and utilizing leaf to extract the cyanogen glycosides is a kind of pattern capable of circulation, and production cost is lower.
In the prior art, the extraction separation of total cyanogen glycosides mainly adopts organic solvent method etc. in the Fructus Sorbi Tianschanicae, but total cyanogen glycosides content that these extraction processes obtain is low, and is seriously polluted in the production process, and is unfavorable for big production operation.
Summary of the invention
The objective of the invention is to overcome the defective and the deficiency of prior art, a kind of method that adopts membrance separation and resin column adsorption technology from Fructus Sorbi Tianschanicae, to extract total cyanogen glycosides is provided, this method energy consumption is low, easy to operate.
The objective of the invention is to realize through following technical scheme:
A kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides is characterized in that described method may further comprise the steps: dry Fructus Sorbi Tianschanicae leaf is pulverized, added doubly 50% alcohol solution dipping of 10-18, squeezing is also filtered; Filtrating adds active carbon insulation decolouring, filters, and reclaims ethanol, and concentrated solution is centrifugal; Supernatant adds appropriate amount of deionized water, through the film separation system ultrafiltration, and macroporous resin column absorption on the permeate, 20-35% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides.
Described ultrafilter membrane is that molecular cut off is the hollow cellulose ultrafilter membrane of 3000-10000.
The model of described macroporous resin is one or more among H-103, AB-8, the DS-401.
The concentration of alcohol of described eluting macroporous resin column is 30%.
Technical advantage of the present invention is that method adopts membrance separation and resin column to remove impurity, carries out under the room temperature, has solved a unsettled difficult problem under the cyanogen glycosides high temperature, and energy consumption is lower, can not pollute product.
To combine the specific embodiment to further specify the present invention below, but the scope that the present invention requires to protect is not limited to the following example.
The specific embodiment
Embodiment 1:
Dry Fructus Sorbi Tianschanicae leaf is pulverized, got 10kg, add 10 times of 50% alcohol solution dipping, squeezing is also filtered; Filtrating adds active carbon insulation decolouring, filters, and reclaims ethanol, and concentrated solution is centrifugal; Supernatant adds appropriate amount of deionized water, is the hollow cellulose ultrafilter membrane ultrafiltration of 3000-10000 through molecular cut off, H-103 type macroporous resin column absorption on the permeate, 30% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides 270g.
Embodiment 2:
Dry Fructus Sorbi Tianschanicae leaf is pulverized, got 10kg, add 15 times of 50% alcohol solution dipping, squeezing is also filtered; Filtrating adds active carbon insulation decolouring, filters, and reclaims ethanol, and concentrated solution is centrifugal; Supernatant adds appropriate amount of deionized water, is the hollow cellulose ultrafilter membrane ultrafiltration of 3000-10000 through molecular cut off, AB-8 type macroporous resin column absorption on the permeate, 30% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides 324g.
Embodiment 3:
Dry Fructus Sorbi Tianschanicae leaf is pulverized, got 10kg, add 15 times of 50% alcohol solution dipping, squeezing is also filtered; Filtrating adds active carbon insulation decolouring, filters, and reclaims ethanol, and concentrated solution is centrifugal; Supernatant adds appropriate amount of deionized water, is the hollow cellulose ultrafilter membrane ultrafiltration of 3000-10000 through molecular cut off, AB-8 type macroporous resin column absorption on the permeate, 30% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides 409g.
Embodiment 4:
Dry Fructus Sorbi Tianschanicae leaf is pulverized, got 20kg, add 18 times of 50% alcohol solution dipping, squeezing is also filtered; Filtrating adds active carbon insulation decolouring, filters, and reclaims ethanol, and concentrated solution is centrifugal; Supernatant adds appropriate amount of deionized water, is the hollow cellulose ultrafilter membrane ultrafiltration of 3000-10000 through molecular cut off, DS-401 type macroporous resin column absorption on the permeate, 30% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides 947g.
Claims (4)
1. a method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides is characterized in that described method may further comprise the steps: dry Fructus Sorbi Tianschanicae leaf is pulverized, added doubly 50% alcohol solution dipping of 10-18, squeeze also and filter; Filtrating adds active carbon insulation decolouring, filters, and reclaims ethanol, and concentrated solution is centrifugal; Supernatant adds appropriate amount of deionized water, through the film separation system ultrafiltration, and macroporous resin column absorption on the permeate, 20-35% alcoholic solution eluting; Collect eluent, concentrating under reduced pressure, spray drying gets total cyanogen glycosides.
2. from Fructus Sorbi Tianschanicae, extract the method for total cyanogen glycosides according to claim 1, it is characterized in that described ultrafilter membrane is that molecular cut off is the hollow cellulose ultrafilter membrane of 3000-10000.
3. from Fructus Sorbi Tianschanicae, extract the method for total cyanogen glycosides according to claim 1, the model that it is characterized in that described macroporous resin is one or more among H-103, AB-8, the DS-401.
4. from Fructus Sorbi Tianschanicae, extract the method for total cyanogen glycosides according to claim 1, the concentration of alcohol that it is characterized in that described eluting macroporous resin column is 30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201110264830A CN102319306A (en) | 2011-09-08 | 2011-09-08 | A kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides |
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| CN201110264830A CN102319306A (en) | 2011-09-08 | 2011-09-08 | A kind of method of from Fructus Sorbi Tianschanicae, extracting total cyanogen glycosides |
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| CN102319306A true CN102319306A (en) | 2012-01-18 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104706755A (en) * | 2015-02-12 | 2015-06-17 | 新疆医科大学 | Antiasthma particles and preparation method thereof |
| CN104739970A (en) * | 2015-02-12 | 2015-07-01 | 新疆医科大学 | Sorbus tianschanica extract powder and preparation method thereof |
| CN110643468A (en) * | 2019-11-22 | 2020-01-03 | 河南省商业科学研究所有限责任公司 | Cornus officinalis-cherry wine and brewing method thereof |
| CN111819947A (en) * | 2020-07-17 | 2020-10-27 | 新疆林业科学院 | Germination accelerating method beneficial to dormancy breaking of sorbus tianshanensis seeds |
-
2011
- 2011-09-08 CN CN201110264830A patent/CN102319306A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104706755A (en) * | 2015-02-12 | 2015-06-17 | 新疆医科大学 | Antiasthma particles and preparation method thereof |
| CN104739970A (en) * | 2015-02-12 | 2015-07-01 | 新疆医科大学 | Sorbus tianschanica extract powder and preparation method thereof |
| CN110643468A (en) * | 2019-11-22 | 2020-01-03 | 河南省商业科学研究所有限责任公司 | Cornus officinalis-cherry wine and brewing method thereof |
| CN111819947A (en) * | 2020-07-17 | 2020-10-27 | 新疆林业科学院 | Germination accelerating method beneficial to dormancy breaking of sorbus tianshanensis seeds |
| CN111819947B (en) * | 2020-07-17 | 2022-03-04 | 新疆农业大学 | Germination accelerating method beneficial to dormancy breaking of sorbus tianshanensis seeds |
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Application publication date: 20120118 |